Rh2AG654800

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007547
Physical Location & Seq
Reverse (-)
87185997 .. 87187179
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG654800.1

Sequence Viewer

Length: 972 bp
ATGGTGAAGTCGTACCTCAGATACGAGCAGGGGGCCGCCTTCGGCGTCATAGTTTCCGGCGGTTCAAACATCACGTACGACGGCTCTGGCAAGCACCTTGTTGCTCCGGCCCTGGAAACGCTGCGCGTGTGGCACGCTCGTCAAGGCATTTGCTCCAAAACCCTAACTCCTTCTCTGGAACATCCTACTTTGGTCACCTGCATTGCGGCCTCACCTTCCACTAACTCCAAAAGTTCTGTAGTTATAGCCTGTGGGTATGGAGATGGTAGTCTAAGAATTTGGGATTCTGACACTGAAACCTGCGACAACATTTTCAATGGTCATAATGGGGCTGTTACTGCCTTACGGTACAATGAGCGTTGGGATATGGTTGCATCTGGAGGCCAAGATAAGGACATTGTATTATGGGATGTGGAGATGGGCGAGGGCAGATATCGTCTCCGCAGGCATGCCGATCAGGTCACTGATCTTGTTTTCTTGGATTCTAGTAACAAACTCGTTAGTTCCTCAAAAGATAAATTGTTGAAGGTGTGGGATCTTGGAAACTTGAATCAAACCCCGTATTGCATGCAGACGATTAGTGGCCATCATAGTGAAATTTGGTCCATAGATAGGGATCCAGATGAAAAGTATTTAGTCTCAGGGTCTGCAGACCGAGAACTAAGATTTTACACAATTAAGCATAACTTGGAAGGTGGCCAATCCATTTCTAATGGGAATGGAACTGAGATTGTGAGCTTTGAAGATAACAAATGGGAAGTTTTGAAGCTGTTTGGTGAATATAATAAGCGGCAAAGCAAGTATAGAGTTGCAACTGTAAGGTTCAACTGGTCTGGAAACTTGCTGGCTTGTCAAGAGGCAGGAAAGACAGTAGAAATATTCAATGTGTTGAATGAGGCTGAATCAAAGCGTAAAGCAAAGCGCAAGCTTCACAGGAAGAAAAGAGAAGAAAAATGCAAAAGGGGAGCCTGA

Protein Analysis

323

Amino Acids

36.26

Weight (kDa)

8.7

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 19 - 182 1.4e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 25 - 181 2.6e-26 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 28 - 225 1.1e-25 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 30 - 224 1.7e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 30 - 227 3e-21 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 31 - 226 1.2e-59 WDR3 first beta-propeller domain
EIF3I PF24805 34 - 185 1.2e-07 EIF3I
Beta-prop_WDR3_2nd PF25172 36 - 137 3e-06 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 40 - 187 1.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 40 - 219 6.7e-11 TEP-1 second beta-propeller
Beta-prop_EML PF23409 43 - 176 5.9e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SCAP PF24017 64 - 180 8.5e-13 SCAP Beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 74 - 228 7e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 75 - 181 5.2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 76 - 225 1.8e-20 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 76 - 180 4e-07 MABP1/WDR62 second WD40 domain
WDR55 PF24796 80 - 227 1e-09 WDR55
WD40_Gbeta PF25391 106 - 228 3e-11 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 113 - 296 7e-20 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 149 - 229 4.1e-08 WDHD1 first WD40 domain
EIF3I PF24805 150 - 274 4.3e-08 EIF3I
Beta-prop_WDR5 PF25175 161 - 228 1.5e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 188 - 231 2.7e-06 WDR3 second beta-propeller domain
WD40 PF00400 189 - 224 5.4e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 2 cut(s) 206, 308
AccII CGCG 1 cut(s) 126
AciI CCGC 5 cut(s) 36, 60, 206, 442, 790
AclWI GGATC 3 cut(s) 543, 611, 624
AcoI YGGCCR 2 cut(s) 583, 697
AcsI RAATTY 2 cut(s) 276, 597
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 14, 77, 350
AfiI CCNNNNNNNGG 2 cut(s) 391, 612
AgsI TTSAA 9 cut(s) 66, 316, 526, 550, 743, 766, 826, 883, 892
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 3 cut(s) 738, 769, 928
AluI AGCT 3 cut(s) 738, 769, 928
Alw26I GTCTC 2 cut(s) 443, 643
AlwI GGATC 3 cut(s) 543, 611, 624
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 6 cut(s) 33, 108, 207, 382, 583, 697
ApeKI GCWGC 1 cut(s) 121
ApoI RAATTY 2 cut(s) 276, 597
AspLEI GCGC 2 cut(s) 126, 924
AspS9I GGNCC 3 cut(s) 33, 109, 603
AsuHPI GGTGA 4 cut(s) 16, 187, 204, 788
AvaII GGWCC 1 cut(s) 603
BalI TGGCCA 2 cut(s) 585, 699
BamHI GGATCC 1 cut(s) 616
BbvI GCAGC 1 cut(s) 108
BccI CCATC 3 cut(s) 257, 412, 594
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 443, 643
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 2 cut(s) 237, 648
BfuAI ACCTGC 2 cut(s) 206, 308
BisI GCNGC 4 cut(s) 36, 122, 207, 791
BlsI GCNGC 4 cut(s) 37, 123, 208, 792
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 603
BmgT120I GGNCC 3 cut(s) 33, 109, 603
BmiI GGNNCC 3 cut(s) 34, 618, 967
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 399
BsaAI YACGTR 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 391, 612
Bse1I ACTGG 1 cut(s) 833
Bse3DI GCAATG 1 cut(s) 201
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 181, 415
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 2 cut(s) 391, 612
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 31, 654, 717
BseNI ACTGG 1 cut(s) 833
BseXI GCAGC 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 6 cut(s) 35, 110, 209, 384, 585, 699
BsiSI CCGG 2 cut(s) 57, 107
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 391, 612
BsmAI GTCTC 2 cut(s) 443, 643
BsmBI CGTCTC 1 cut(s) 443
BsnI GGCC 6 cut(s) 35, 110, 209, 384, 585, 699
Bsp143I GATC 4 cut(s) 454, 466, 535, 616
BspACI CCGC 5 cut(s) 36, 60, 206, 442, 790
BspANI GGCC 6 cut(s) 35, 110, 209, 384, 585, 699
BspCNI CTCAG 3 cut(s) 30, 653, 718
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 34, 618, 967
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 206, 308
BspPI GGATC 3 cut(s) 543, 611, 624
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 1 cut(s) 833
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 454, 466, 535, 616
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 3 cut(s) 348, 817, 871
BstACI GRCGYC 1 cut(s) 45
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 7 cut(s) 92, 135, 446, 450, 569, 846, 926
BstDEI CTNAG 5 cut(s) 17, 272, 640, 662, 726
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 2 cut(s) 181, 415
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 2 cut(s) 126, 924
BstKTI GATC 4 cut(s) 457, 469, 538, 619
BstMAI GTCTC 2 cut(s) 443, 643
BstMBI GATC 4 cut(s) 454, 466, 535, 616
BstMWI GCNNNNNNNGC 2 cut(s) 130, 338
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 452, 571
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 237, 648
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 108
BstX2I RGATCY 2 cut(s) 535, 616
BstYI RGATCY 2 cut(s) 535, 616
BsuRI GGCC 6 cut(s) 35, 110, 209, 384, 585, 699
BtsCI GGATG 2 cut(s) 181, 415
BtsIMutI CAGTG 2 cut(s) 291, 462
BveI ACCTGC 2 cut(s) 206, 308
Cac8I GCNNGC 7 cut(s) 92, 135, 446, 450, 569, 846, 926
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 2 cut(s) 126, 924
Cfr13I GGNCC 3 cut(s) 33, 109, 603
CseI GACGC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 13, 76, 349
CviAII CATG 2 cut(s) 449, 568
CviQI GTAC 3 cut(s) 13, 76, 349
DdeI CTNAG 5 cut(s) 17, 272, 640, 662, 726
DpnI GATC 4 cut(s) 456, 468, 537, 618
DpnII GATC 4 cut(s) 454, 466, 535, 616
EaeI YGGCCR 2 cut(s) 583, 697
Eco32I GATATC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 603
Eco91I GGTNACC 1 cut(s) 193
EcoO65I GGTNACC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 111
EcoRV GATATC 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 2 cut(s) 452, 571
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FatI CATG 2 cut(s) 448, 567
Fnu4HI GCNGC 4 cut(s) 36, 122, 207, 791
FokI GGATG 2 cut(s) 168, 422
Fsp4HI GCNGC 4 cut(s) 36, 122, 207, 791
FspBI CTAG 1 cut(s) 486
GlaI GCGC 2 cut(s) 125, 923
GluI GCNGC 4 cut(s) 36, 122, 207, 791
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 6 cut(s) 35, 110, 209, 384, 585, 699
HapII CCGG 2 cut(s) 57, 107
HgaI GACGC 1 cut(s) 34
HhaI GCGC 2 cut(s) 126, 924
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 2 cut(s) 452, 571
Hin6I GCGC 2 cut(s) 124, 922
HinP1I GCGC 2 cut(s) 124, 922
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 4 cut(s) 284, 482, 550, 902
HpaII CCGG 2 cut(s) 57, 107
HphI GGTGA 4 cut(s) 16, 187, 204, 788
Hpy188I TCNGA 2 cut(s) 20, 289
Hpy188III TCNNGA 5 cut(s) 176, 378, 620, 834, 854
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 5 cut(s) 49, 180, 225, 520, 686
HpyCH4III ACNGT 3 cut(s) 348, 817, 871
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 7 cut(s) 201, 374, 567, 571, 650, 812, 957
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 338
HpyF3I CTNAG 5 cut(s) 17, 272, 640, 662, 726
HpySE526I ACGT 1 cut(s) 74
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 2 cut(s) 452, 571
HspAI GCGC 2 cut(s) 124, 922
Kzo9I GATC 4 cut(s) 454, 466, 535, 616
LmnI GCTCC 3 cut(s) 109, 158, 965
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 383
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 4 cut(s) 193, 334, 460, 488
MalI GATC 4 cut(s) 456, 468, 537, 618
MboI GATC 4 cut(s) 454, 466, 535, 616
MboII GAAGA 3 cut(s) 755, 949, 959
MflI RGATCY 2 cut(s) 535, 616
MlsI TGGCCA 2 cut(s) 585, 699
MluCI AATT 4 cut(s) 276, 518, 597, 675
MluNI TGGCCA 2 cut(s) 585, 699
MnlI CCTC 7 cut(s) 26, 220, 374, 418, 517, 850, 889
Mox20I TGGCCA 2 cut(s) 585, 699
MscI TGGCCA 2 cut(s) 585, 699
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 591
Msp20I TGGCCA 2 cut(s) 585, 699
MspI CCGG 2 cut(s) 57, 107
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 130, 338
NdeII GATC 4 cut(s) 454, 466, 535, 616
NlaIII CATG 2 cut(s) 452, 571
NlaIV GGNNCC 3 cut(s) 34, 618, 967
NmuCI GTSAC 2 cut(s) 193, 460
NspI RCATGY 2 cut(s) 452, 571
PaeI GCATGC 2 cut(s) 452, 571
PaqCI CACCTGC 1 cut(s) 206
PfeI GAWTC 4 cut(s) 284, 482, 550, 902
Pfl23II CGTACG 1 cut(s) 75
PkrI GCNGC 4 cut(s) 37, 123, 208, 792
Ppu21I YACGTR 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 111
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 34, 618, 967
PspPI GGNCC 3 cut(s) 33, 109, 603
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 2 cut(s) 535, 616
RsaI GTAC 3 cut(s) 14, 77, 350
RsaNI GTAC 3 cut(s) 13, 76, 349
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 4 cut(s) 36, 122, 207, 791
Sau3AI GATC 4 cut(s) 454, 466, 535, 616
Sau96I GGNCC 3 cut(s) 33, 109, 603
ScrFI CCNGG 1 cut(s) 113
SfaNI GCATC 1 cut(s) 383
SfcI CTRYAG 2 cut(s) 237, 648
SinI GGWCC 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 591
SphI GCATGC 2 cut(s) 452, 571
Sse9I AATT 4 cut(s) 276, 518, 597, 675
SsiI CCGC 5 cut(s) 36, 60, 206, 442, 790
SspI AATATT 1 cut(s) 879
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 348, 817, 871
TaiI ACGT 1 cut(s) 77
TaqII GACCGA 1 cut(s) 669
TasI AATT 4 cut(s) 276, 518, 597, 675
TauI GCSGC 3 cut(s) 38, 209, 793
TfiI GAWTC 4 cut(s) 284, 482, 550, 902
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 2 cut(s) 298, 469
TseFI GTSAC 2 cut(s) 193, 460
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 2 cut(s) 193, 460
TspDTI ATGAA 1 cut(s) 639
TspRI CASTG 2 cut(s) 298, 469
VpaK11BI GGWCC 1 cut(s) 603
XapI RAATTY 2 cut(s) 276, 597
XceI RCATGY 2 cut(s) 452, 571
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.