Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0002675

Physical Location & Seq

Genomic Coordinates Forward (+)

39734919 .. 39737821

Gene Structure 2903 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0337500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGATTTGGTTACAGAGCATATCCAAGCTTCAGGCAGCTTTGGTTAAGGCCGAGGATAATCTTTCTACACTTCCATCTGAGCCACCCTATTGTGAGTTTGAATATGTATTACAAGGATTGGGTTTTGAGCGAGGTTGGGGAGACACTGCAGCACAGGTATTGGAGTTGATGCATCTTCTCCGAGATATCCTACAGGCCCCTGATCCTACTACCTTGAAGACATTTCTAGGGAGAGTACCAATGGTTTTTAATGTTGTCATTTTGTCTCCACATGGATACTTTGGGCAAGCGAATGTCTTAGGCTTACCTGACACCGATGGGCAGGTTGTCTATATACTGGACCAAGTGTGTGCCCTCGAGAATGAAAGGCGTCAAAGAGTCACTGGAACTACATGCAACCAGCGGCTAGAAAGAGTCACTAGAACAGAGCACAGCCATATTCTGCGAGTTCCTTTCAGATCAGAGAATGGAATTCTTCGTAAATGGATATCAAGGTTTGATGTATGGTCTTATTTGGAGACCTTTGCTGAGGATGTTGCAGATACTGGAGGTGCTTTTGTTCAGCTTGCACTTTATGAAGCTTTTGGACTCACAGTTGTGGAGGCCATGACTTGTGGCCTTCCGACATTTGCCACAATCCACGGTGGTCCTGCAGAGATCATTGAGCACGGTATATCCGGATTTCATATCGACCCTTATCACTCTGAACAGATGGCTGCAATTGTGGCTGATTTCTTTCAATGTAGTAAGGAGGATCCTAGCTACTGGAAGAAAATTACAGATGGGGGCCTGAAAAGAATTCCTAAAGACTTTGATGTGATCACTACAGCTAATCTTAAGCAGATCAAGAGGCAATTCCATCGTGCACAAATTGTCGGACAACGATTTCCTATATGTATGGTGCATATTAAAGGTTCTGTCATAGAGGTATCCAGTTTTGAAACGGTGGCAAAAGCAAAACACTCTAATAAAGAAGTTACTTTCTCTCAAATGCCAAAGGGTTGTGATAAGAAAGATTTCATCCGCTGGAGAAACAGCATGCATCGAGACTTCACAATTAACAGTTTGTTCTTTGATCCTTTATCAAATAAAATCTTTGATTATGCAAATGGGATGGCAGATTTGAGATCGATATCCTTAAAGCTACAGTCGTTAGGCTCCGCCAAGTTGTCATTTAAAGAGGATTGCGCAAGAATTTTACGTGGCTTAAGAATTGCTGCTCGTCTGAGTTTGTCAATTCCAAAAGACATAAGTTTTCTTCATCCATTTCGACATTGGCAACGAGTAGAATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004514 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009435 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016740 GO:0016757 GO:0016763 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019637 GO:0019674 GO:0019752 GO:0034213 GO:0034641 GO:0034654 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046483 GO:0046496 GO:0046700 GO:0046874 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072524 GO:0072525 GO:0072526 GO:0090407 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00760 ko01100 map00760 map01100

Pfam Domains

Protein Families

QRPTase_C QRPTase_N

Protein Analysis

431

Amino Acids

48.87

Weight (kDa)

8.06

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_EPBD	PF24862	4 - 48	2.6e-10	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	71 - 127	1.1e-23	Sucrose synthase first GT-B domain
GT-B_Sucrose_synth	PF00862	127 - 181	5.6e-22	Sucrose synthase first GT-B domain
Glyco_trans_1_4	PF13692	181 - 244	4.2e-10	Glycosyl transferases group 1
Glycos_transf_1	PF00534	185 - 257	1.6e-16	Glycosyl transferases group 1
PolyA_pol	PF01743	261 - 377	4e-16	Poly A polymerase head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01350 AT2G01350 AT2G01350 AT2G01350
fragaria_vesca	FvH4_4g32330 FvH4_4g32330 FvH4_4g32330 FvH4_4g32330
malus_domestica	MD16G1029000.v1.1
prunus_persica	Prupe.1G323900_v2.0.a1 Prupe.1G323900_v2.0.a1 Prupe.1G323900_v2.0.a1 Prupe.1G323900_v2.0.a1 Prupe.1G323900_v2.0.a1
pyrus_communis	pycom16g02470
rosa_chinensis	RchiOBHm_Chr2g0157601 RchiOBHm_Chr4g0441121
rosa_laevigata	RLG00000006112
rosa_multiflora	Rmu_sc0000607.1_g000026 Rmu_sc0006317.1_g000018 Rmu_sc0017059.1_g000001
rosa_roxburghii	Rroxscaffold_5G00382090
rosa_rugosa	Rorug03G0337500 Rorug04G0331400
rosa_samantha	Rh1AG340100 Rh2BG059800 Rh2BG243400 Rh2BG348100 Rh2DG286800 Rh3BG365900 Rh4AG382900 Rh4BG396800 Rh4CG411800 Rh4DG389700 Rh5BG215200 Rh5BG425100 Rh6BG216200 Rh6DG027200
rosa_wichuraiana	Rw4G033050 Rw6G003900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1189
Acc36I	ACCTGC	1 cut(s)	313
AccIII	TCCGGA	1 cut(s)	677
AciI	CCGC	3 cut(s)	403, 1024, 1161
AclWI	GGATC	4 cut(s)	197, 749, 762, 1070
AcsI	RAATTY	3 cut(s)	471, 798, 1194
AcuI	CTGAAG	1 cut(s)	14
AcyI	GRCGYC	1 cut(s)	370
AfaI	GTAC	1 cut(s)	237
AflII	CTTAAG	2 cut(s)	836, 1207
AgsI	TTSAA	4 cut(s)	101, 217, 740, 941
AleI	CACNNNNGTG	1 cut(s)	596
AluBI	AGCT	7 cut(s)	28, 38, 565, 581, 762, 830, 1144
AluI	AGCT	7 cut(s)	28, 38, 565, 581, 762, 830, 1144
Alw21I	GWGCWC	3 cut(s)	432, 669, 868
Alw26I	GTCTC	4 cut(s)	135, 270, 512, 1041
Alw44I	GTGCAC	1 cut(s)	864
AlwI	GGATC	4 cut(s)	197, 749, 762, 1070
AlwNI	CAGNNNCTG	1 cut(s)	545
Ama87I	CYCGRG	1 cut(s)	356
Aor13HI	TCCGGA	1 cut(s)	677
AoxI	GGCC	5 cut(s)	48, 195, 603, 616, 787
ApaLI	GTGCAC	1 cut(s)	864
ApeKI	GCWGC	4 cut(s)	35, 149, 716, 1217
ApoI	RAATTY	3 cut(s)	471, 798, 1194
Asp700I	GAANNNNTTC	1 cut(s)	1016
AspLEI	GCGC	1 cut(s)	1190
AspS9I	GGNCC	4 cut(s)	196, 340, 647, 787
AvaI	CYCGRG	1 cut(s)	356
AvaII	GGWCC	2 cut(s)	340, 647
BaeGI	GKGCMC	2 cut(s)	355, 868
BamHI	GGATCC	1 cut(s)	754
BbsI	GAAGAC	1 cut(s)	224
Bbv12I	GWGCWC	3 cut(s)	432, 669, 868
BbvCI	CCTCAGC	1 cut(s)	528
BbvI	GCAGC	4 cut(s)	47, 161, 703, 1204
BccI	CCATC	6 cut(s)	82, 311, 706, 776, 867, 1108
BcgI	CGANNNNNNTGC	2 cut(s)	842, 876
BciVI	GTATCC	2 cut(s)	269, 940
BclI	TGATCA	1 cut(s)	819
BcoDI	GTCTC	4 cut(s)	135, 270, 512, 1041
BfaI	CTAG	4 cut(s)	227, 407, 420, 759
BfmI	CTRYAG	5 cut(s)	147, 191, 651, 825, 1145
BfrI	CTTAAG	2 cut(s)	836, 1207
BfuAI	ACCTGC	1 cut(s)	313
BfuI	GTATCC	2 cut(s)	269, 940
BisI	GCNGC	5 cut(s)	36, 150, 404, 717, 1218
BlsI	GCNGC	5 cut(s)	37, 151, 405, 718, 1219
Bme18I	GGWCC	2 cut(s)	340, 647
BmeT110I	CYCGRG	1 cut(s)	356
BmgT120I	GGNCC	4 cut(s)	196, 340, 647, 787
BmiI	GGNNCC	4 cut(s)	198, 756, 788, 1159
BmsI	GCATC	3 cut(s)	159, 181, 1051
BpiI	GAAGAC	1 cut(s)	224
BpmI	CTGGAG	2 cut(s)	567, 1048
Bpu10I	CCTNAGC	1 cut(s)	528
Bsa29I	ATCGAT	1 cut(s)	1130
BsaAI	YACGTR	1 cut(s)	1202
BsaBI	GATNNNNATC	1 cut(s)	1132
BsaHI	GRCGYC	1 cut(s)	370
BsaI	GGTCTC	1 cut(s)	512
BsaJI	CCNNGG	2 cut(s)	51, 640
BsaWI	WCCGGW	1 cut(s)	677
Bse1I	ACTGG	5 cut(s)	342, 388, 550, 770, 933
Bse8I	GATNNNNATC	1 cut(s)	1132
BseAI	TCCGGA	1 cut(s)	677
BseCI	ATCGAT	1 cut(s)	1130
BseDI	CCNNGG	2 cut(s)	51, 640
BseGI	GGATG	4 cut(s)	538, 1020, 1119, 1261
BseJI	GATNNNNATC	1 cut(s)	1132
BseMII	CTCAG	3 cut(s)	69, 519, 1217
BseNI	ACTGG	5 cut(s)	342, 388, 550, 770, 933
BseSI	GKGCMC	2 cut(s)	355, 868
BseXI	GCAGC	4 cut(s)	47, 161, 703, 1204
BshFI	GGCC	5 cut(s)	50, 197, 605, 618, 789
BshVI	ATCGAT	1 cut(s)	1130
BsiHKAI	GWGCWC	3 cut(s)	432, 669, 868
BsiHKCI	CYCGRG	1 cut(s)	356
BsiSI	CCGG	1 cut(s)	678
BsmAI	GTCTC	4 cut(s)	135, 270, 512, 1041
BsnI	GGCC	5 cut(s)	50, 197, 605, 618, 789
Bso31I	GGTCTC	1 cut(s)	512
BsoBI	CYCGRG	1 cut(s)	356
Bsp1286I	GDGCHC	4 cut(s)	355, 432, 669, 868
Bsp13I	TCCGGA	1 cut(s)	677
Bsp143I	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
BspACI	CCGC	3 cut(s)	403, 1024, 1161
BspANI	GGCC	5 cut(s)	50, 197, 605, 618, 789
BspCNI	CTCAG	3 cut(s)	70, 520, 1218
BspDI	ATCGAT	1 cut(s)	1130
BspEI	TCCGGA	1 cut(s)	677
BspLI	GGNNCC	4 cut(s)	198, 756, 788, 1159
BspMAI	CTGCAG	2 cut(s)	151, 655
BspMI	ACCTGC	1 cut(s)	313
BspPI	GGATC	4 cut(s)	197, 749, 762, 1070
BspTI	CTTAAG	2 cut(s)	836, 1207
BspTNI	GGTCTC	1 cut(s)	512
BsrI	ACTGG	5 cut(s)	342, 388, 550, 770, 933
BssECI	CCNNGG	2 cut(s)	51, 640
BssMI	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
BssNI	GRCGYC	1 cut(s)	370
Bst4CI	ACNGT	6 cut(s)	595, 644, 671, 946, 1064, 1149
BstACI	GRCGYC	1 cut(s)	370
BstAFI	CTTAAG	2 cut(s)	836, 1207
BstBAI	YACGTR	1 cut(s)	1202
BstC8I	GCNNGC	3 cut(s)	288, 567, 1040
BstDEI	CTNAG	4 cut(s)	78, 298, 528, 1226
BstDSI	CCRYGG	1 cut(s)	640
BstF5I	GGATG	4 cut(s)	538, 1020, 1119, 1261
BstHHI	GCGC	1 cut(s)	1190
BstKTI	GATC	8 cut(s)	205, 461, 660, 757, 822, 846, 1078, 1130
BstMAI	GTCTC	4 cut(s)	135, 270, 512, 1041
BstMBI	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
BstMWI	GCNNNNNNNGC	1 cut(s)	725
BstNSI	RCATGY	2 cut(s)	396, 1042
BstSFI	CTRYAG	5 cut(s)	147, 191, 651, 825, 1145
BstSLI	GKGCMC	2 cut(s)	355, 868
BstV1I	GCAGC	4 cut(s)	47, 161, 703, 1204
BstV2I	GAAGAC	1 cut(s)	224
BstX2I	RGATCY	1 cut(s)	754
BstYI	RGATCY	1 cut(s)	754
Bsu15I	ATCGAT	1 cut(s)	1130
BsuI	GTATCC	2 cut(s)	269, 940
BsuRI	GGCC	5 cut(s)	50, 197, 605, 618, 789
BsuTUI	ATCGAT	1 cut(s)	1130
BtgI	CCRYGG	1 cut(s)	640
BtsCI	GGATG	4 cut(s)	538, 1020, 1119, 1261
BtsI	GCAGTG	1 cut(s)	144
BtsIMutI	CAGTG	2 cut(s)	144, 381
BveI	ACCTGC	1 cut(s)	313
Cac8I	GCNNGC	3 cut(s)	288, 567, 1040
CaiI	CAGNNNCTG	1 cut(s)	545
CfoI	GCGC	1 cut(s)	1190
Cfr13I	GGNCC	4 cut(s)	196, 340, 647, 787
ClaI	ATCGAT	1 cut(s)	1130
CseI	GACGC	1 cut(s)	359
Csp6I	GTAC	1 cut(s)	236
CviAII	CATG	4 cut(s)	272, 393, 607, 1039
CviQI	GTAC	1 cut(s)	236
DdeI	CTNAG	4 cut(s)	78, 298, 528, 1226
DpnI	GATC	8 cut(s)	204, 460, 659, 756, 821, 845, 1077, 1129
DpnII	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
DraI	TTTAAA	1 cut(s)	1177
EciI	GGCGGA	1 cut(s)	1150
Eco31I	GGTCTC	1 cut(s)	512
Eco32I	GATATC	3 cut(s)	187, 489, 1134
Eco47I	GGWCC	2 cut(s)	340, 647
Eco57I	CTGAAG	1 cut(s)	14
Eco88I	CYCGRG	1 cut(s)	356
EcoO109I	RGGNCCY	2 cut(s)	196, 787
EcoRI	GAATTC	2 cut(s)	471, 798
EcoRV	GATATC	3 cut(s)	187, 489, 1134
EcoT22I	ATGCAT	2 cut(s)	174, 1044
FaeI	CATG	4 cut(s)	275, 396, 610, 1042
FatI	CATG	4 cut(s)	271, 392, 606, 1038
FbaI	TGATCA	1 cut(s)	819
Fnu4HI	GCNGC	5 cut(s)	36, 150, 404, 717, 1218
FokI	GGATG	4 cut(s)	545, 1007, 1126, 1248
Fsp4HI	GCNGC	5 cut(s)	36, 150, 404, 717, 1218
FspBI	CTAG	4 cut(s)	227, 407, 420, 759
FspI	TGCGCA	1 cut(s)	1189
GlaI	GCGC	1 cut(s)	1189
GluI	GCNGC	5 cut(s)	36, 150, 404, 717, 1218
GsuI	CTGGAG	2 cut(s)	567, 1048
HaeIII	GGCC	5 cut(s)	50, 197, 605, 618, 789
HapII	CCGG	1 cut(s)	678
HgaI	GACGC	1 cut(s)	359
HhaI	GCGC	1 cut(s)	1190
Hin1I	GRCGYC	1 cut(s)	370
Hin1II	CATG	4 cut(s)	275, 396, 610, 1042
Hin6I	GCGC	1 cut(s)	1188
HinP1I	GCGC	1 cut(s)	1188
HindIII	AAGCTT	2 cut(s)	26, 579
HinfI	GANTC	3 cut(s)	378, 414, 588
HpaII	CCGG	1 cut(s)	678
Hpy166II	GTNNAC	1 cut(s)	866
Hpy188I	TCNGA	8 cut(s)	79, 182, 458, 463, 624, 706, 878, 1227
Hpy188III	TCNNGA	4 cut(s)	358, 678, 847, 1046
Hpy8I	GTNNAC	1 cut(s)	866
HpyAV	CCTTC	1 cut(s)	629
HpyCH4III	ACNGT	6 cut(s)	595, 644, 671, 946, 1064, 1149
HpyCH4IV	ACGT	1 cut(s)	1201
HpyF10VI	GCNNNNNNNGC	1 cut(s)	725
HpyF3I	CTNAG	4 cut(s)	78, 298, 528, 1226
HpySE526I	ACGT	1 cut(s)	1201
Hsp92I	GRCGYC	1 cut(s)	370
Hsp92II	CATG	4 cut(s)	275, 396, 610, 1042
HspAI	GCGC	1 cut(s)	1188
Kpn2I	TCCGGA	1 cut(s)	677
Ksp22I	TGATCA	1 cut(s)	819
Kzo9I	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
LmnI	GCTCC	1 cut(s)	1163
Lsp1109I	GCAGC	4 cut(s)	47, 161, 703, 1204
LweI	GCATC	3 cut(s)	159, 181, 1051
MaeI	CTAG	4 cut(s)	227, 407, 420, 759
MaeII	ACGT	1 cut(s)	1201
MaeIII	GTNAC	4 cut(s)	9, 379, 415, 976
MalI	GATC	8 cut(s)	204, 460, 659, 756, 821, 845, 1077, 1129
MboI	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
MboII	GAAGA	5 cut(s)	167, 229, 467, 781, 1250
MfeI	CAATTG	1 cut(s)	720
MflI	RGATCY	1 cut(s)	754
MhlI	GDGCHC	4 cut(s)	355, 432, 669, 868
MlyI	GAGTC	3 cut(s)	387, 423, 582
MmeI	TCCRAC	2 cut(s)	647, 856
Mph1103I	ATGCAT	2 cut(s)	174, 1044
MroI	TCCGGA	1 cut(s)	677
MroXI	GAANNNNTTC	1 cut(s)	1016
MseI	TTAA	8 cut(s)	45, 249, 837, 909, 1059, 1139, 1176, 1208
MslI	CAYNNNNRTG	1 cut(s)	596
MspA1I	CMGCKG	2 cut(s)	403, 1026
MspCI	CTTAAG	2 cut(s)	836, 1207
MspI	CCGG	1 cut(s)	678
MunI	CAATTG	1 cut(s)	720
MwoI	GCNNNNNNNGC	1 cut(s)	725
NdeII	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
NlaIII	CATG	4 cut(s)	275, 396, 610, 1042
NlaIV	GGNNCC	4 cut(s)	198, 756, 788, 1159
NmeAIII	GCCGAG	1 cut(s)	76
NmuCI	GTSAC	2 cut(s)	379, 415
NsbI	TGCGCA	1 cut(s)	1189
NsiI	ATGCAT	2 cut(s)	174, 1044
NspI	RCATGY	2 cut(s)	396, 1042
OliI	CACNNNNGTG	1 cut(s)	596
PaeI	GCATGC	1 cut(s)	1042
PaeR7I	CTCGAG	1 cut(s)	356
PdmI	GAANNNNTTC	1 cut(s)	1016
PkrI	GCNGC	5 cut(s)	37, 151, 405, 718, 1219
PleI	GAGTC	3 cut(s)	386, 422, 582
PpsI	GAGTC	3 cut(s)	386, 422, 582
Ppu21I	YACGTR	1 cut(s)	1202
PspN4I	GGNNCC	4 cut(s)	198, 756, 788, 1159
PspPI	GGNCC	4 cut(s)	196, 340, 647, 787
PstI	CTGCAG	2 cut(s)	151, 655
PstNI	CAGNNNCTG	1 cut(s)	545
PsuI	RGATCY	1 cut(s)	754
RsaI	GTAC	1 cut(s)	237
RsaNI	GTAC	1 cut(s)	236
RseI	CAYNNNNRTG	1 cut(s)	596
SaqAI	TTAA	8 cut(s)	45, 249, 837, 909, 1059, 1139, 1176, 1208
SatI	GCNGC	5 cut(s)	36, 150, 404, 717, 1218
Sau3AI	GATC	8 cut(s)	202, 458, 657, 754, 819, 843, 1075, 1127
Sau96I	GGNCC	4 cut(s)	196, 340, 647, 787
SchI	GAGTC	3 cut(s)	387, 423, 582
SduI	GDGCHC	4 cut(s)	355, 432, 669, 868
SfaNI	GCATC	3 cut(s)	159, 181, 1051
SfcI	CTRYAG	5 cut(s)	147, 191, 651, 825, 1145
Sfr274I	CTCGAG	1 cut(s)	356
SinI	GGWCC	2 cut(s)	340, 647
SlaI	CTCGAG	1 cut(s)	356
SmiMI	CAYNNNNRTG	1 cut(s)	596
SmlI	CTYRAG	3 cut(s)	356, 836, 1207
SmoI	CTYRAG	3 cut(s)	356, 836, 1207
SphI	GCATGC	1 cut(s)	1042
SsiI	CCGC	3 cut(s)	403, 1024, 1161
SspMI	CTAG	4 cut(s)	227, 407, 420, 759
TaaI	ACNGT	6 cut(s)	595, 644, 671, 946, 1064, 1149
TaiI	ACGT	1 cut(s)	1204
TaqI	TCGA	5 cut(s)	357, 690, 1045, 1130, 1270
TauI	GCSGC	1 cut(s)	406
Tru1I	TTAA	8 cut(s)	45, 249, 837, 909, 1059, 1139, 1176, 1208
Tru9I	TTAA	8 cut(s)	45, 249, 837, 909, 1059, 1139, 1176, 1208
TscAI	CASTG	2 cut(s)	151, 388
TseFI	GTSAC	2 cut(s)	379, 415
TseI	GCWGC	4 cut(s)	35, 149, 716, 1217
Tsp45I	GTSAC	2 cut(s)	379, 415
TspDTI	ATGAA	5 cut(s)	378, 591, 674, 1009, 1250
TspRI	CASTG	2 cut(s)	151, 388
Vha464I	CTTAAG	2 cut(s)	836, 1207
VneI	GTGCAC	1 cut(s)	864
VpaK11BI	GGWCC	2 cut(s)	340, 647
XapI	RAATTY	3 cut(s)	471, 798, 1194
XceI	RCATGY	2 cut(s)	396, 1042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1272
XhoI	CTCGAG	1 cut(s)	356
XmnI	GAANNNNTTC	1 cut(s)	1016
XspI	CTAG	4 cut(s)	227, 407, 420, 759
Zsp2I	ATGCAT	2 cut(s)	174, 1044

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.