Rorug05G0102500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006912
Physical Location & Seq
Reverse (-)
9017164 .. 9019277
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0102500.1

Sequence Viewer

Length: 768 bp
ATGGGAGAAGAAGAAGAAGAGCCCAAGAAGAAGAGGGTGGTGGTGGAATCCCTGGGATGGCTTACGGAGTCGACCATAATGCCCAAGAAGCACCGCGCCATCGCCGGCGTCGGAGCCTCTTCGATCATGGAGCTCAAGGCCCAGCTCTACCAGTCTCAAGAAGACTCCAAGAAGTCCAAGGACCTCGCCGGCCCAGACGCCGAGTTCCACCGCGCCAAGAAGAAGATCACCGCACACGATGCCTTCTCCGTCAAGAACTCCGGCGTCGACGCCCGGGCCCACAAGGACAAGCTTGAGCTGAAGGCAGTTAAGGATGGATCAGCCAGCTATGCAGCGTTGGAGAAAAAGGCTGCATTGTATGATAAGCTAGCGAGGGGAGAGCTATCCGATGAGGAAGACAAGGAAAAGTACTGTGTGGATTTCTTTGTCAAGAGACATGAGCAGGATGAGTCAGAGCAGACTCAGCATCACGATTCTTCTGTTGTTGCACCACCGGAGAATCAAGATGGCGAGACTGATCTTTCCATGTTGTTTAGCACAAAACCTGTGGGGCTTGGGCAGGCAGGTGCAATATGGGACAATGATGAACACAAGCGATTTGTGAGGGAAGTTCATCAAGAAGCGAATCAAGCAAGAGAGAAAGCCTCAGAGCTCAAAGTACGCAGGCAAGAGCAAGCAACTGCTCGTCGTGAGAAGTTAAAACAAGCATACATACGGAAACAGCTAGAGAAACTAAAAGCAGCAGCACCTAATAAGGAACAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.78

Weight (kDa)

7.15

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 554
AccI GTMKAC 2 cut(s) 71, 267
AccII CGCG 2 cut(s) 96, 213
AciI CCGC 3 cut(s) 94, 211, 231
AclWI GGATC 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 4 cut(s) 108, 198, 264, 270
AfaI GTAC 2 cut(s) 410, 660
AfiI CCNNNNNNNGG 1 cut(s) 57
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 9 cut(s) 133, 145, 292, 298, 327, 367, 382, 652, 724
AluI AGCT 9 cut(s) 133, 145, 292, 298, 327, 367, 382, 652, 724
Alw21I GWGCWC 2 cut(s) 135, 654
Alw26I GTCTC 3 cut(s) 159, 427, 506
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 3 cut(s) 138, 190, 276
ApaI GGGCCC 1 cut(s) 280
ApeKI GCWGC 4 cut(s) 332, 350, 740, 743
AspLEI GCGC 2 cut(s) 98, 215
AspS9I GGNCC 5 cut(s) 139, 181, 191, 276, 277
AsuC2I CCSGG 2 cut(s) 274, 275
AsuHPI GGTGA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 280
BanII GRGCYC 4 cut(s) 24, 135, 280, 654
BbsI GAAGAC 2 cut(s) 168, 402
Bbv12I GWGCWC 2 cut(s) 135, 654
BbvI GCAGC 4 cut(s) 337, 344, 752, 755
BccI CCATC 4 cut(s) 51, 107, 308, 500
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 2 cut(s) 274, 275
BcoDI GTCTC 3 cut(s) 159, 427, 506
BfaI CTAG 2 cut(s) 368, 725
BfuAI ACCTGC 1 cut(s) 554
BisI GCNGC 4 cut(s) 333, 351, 741, 744
BlsI GCNGC 4 cut(s) 334, 352, 742, 745
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 3 cut(s) 53, 274, 275
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 5 cut(s) 139, 181, 191, 276, 277
BmiI GGNNCC 2 cut(s) 115, 278
BmrFI CCNGG 3 cut(s) 53, 274, 275
BmsI GCATC 2 cut(s) 229, 475
BmtI GCTAGC 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 168, 402
BplI GAGNNNNNCTC 2 cut(s) 629, 661
BpuEI CTTGAG 3 cut(s) 119, 141, 314
BpuMI CCSGG 2 cut(s) 274, 275
BsaHI GRCGYC 4 cut(s) 108, 198, 264, 270
BsaJI CCNNGG 4 cut(s) 51, 52, 177, 273
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 2 cut(s) 104, 188
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 51, 52, 177, 273
BseGI GGATG 3 cut(s) 62, 319, 451
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 476, 660
BseNI ACTGG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 4 cut(s) 337, 344, 752, 755
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 96, 213
BshFI GGCC 3 cut(s) 140, 192, 278
BsiHKAI GWGCWC 2 cut(s) 135, 654
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 5 cut(s) 105, 189, 261, 274, 494
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 3 cut(s) 159, 427, 506
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 3 cut(s) 140, 192, 278
BsoBI CYCGRG 1 cut(s) 273
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 4 cut(s) 24, 135, 280, 654
Bsp143I GATC 4 cut(s) 123, 225, 317, 517
BspACI CCGC 3 cut(s) 94, 211, 231
BspANI GGCC 3 cut(s) 140, 192, 278
BspCNI CTCAG 2 cut(s) 475, 659
BspFNI CGCG 2 cut(s) 96, 213
BspLI GGNNCC 2 cut(s) 115, 278
BspMI ACCTGC 1 cut(s) 554
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 104, 188
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 2 cut(s) 104, 188
BssECI CCNNGG 4 cut(s) 51, 52, 177, 273
BssMI GATC 4 cut(s) 123, 225, 317, 517
BssNI GRCGYC 4 cut(s) 108, 198, 264, 270
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 413
Bst6I CTCTTC 3 cut(s) 12, 26, 124
BstACI GRCGYC 4 cut(s) 108, 198, 264, 270
BstAPI GCANNNNNTGC 1 cut(s) 239
BstC8I GCNNGC 7 cut(s) 106, 190, 325, 369, 561, 665, 675
BstDEI CTNAG 2 cut(s) 462, 646
BstF5I GGATG 3 cut(s) 62, 319, 451
BstFNI CGCG 2 cut(s) 96, 213
BstHHI GCGC 2 cut(s) 98, 215
BstKTI GATC 4 cut(s) 126, 228, 320, 520
BstMAI GTCTC 3 cut(s) 159, 427, 506
BstMBI GATC 4 cut(s) 123, 225, 317, 517
BstMWI GCNNNNNNNGC 5 cut(s) 88, 239, 329, 463, 629
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 51, 272, 273
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 2 cut(s) 96, 213
BstV1I GCAGC 4 cut(s) 337, 344, 752, 755
BstV2I GAAGAC 2 cut(s) 168, 402
BsuRI GGCC 3 cut(s) 140, 192, 278
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 62, 319, 451
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 7 cut(s) 106, 190, 325, 369, 561, 665, 675
CfoI GCGC 2 cut(s) 98, 215
Cfr10I RCCGGY 2 cut(s) 104, 188
Cfr13I GGNCC 5 cut(s) 139, 181, 191, 276, 277
Cfr9I CCCGGG 1 cut(s) 273
CseI GACGC 4 cut(s) 97, 206, 253, 278
Csp6I GTAC 2 cut(s) 409, 659
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 3 cut(s) 127, 437, 526
CviQI GTAC 2 cut(s) 409, 659
DdeI CTNAG 2 cut(s) 462, 646
DpnI GATC 4 cut(s) 125, 227, 319, 519
DpnII GATC 4 cut(s) 123, 225, 317, 517
Eam1104I CTCTTC 3 cut(s) 12, 26, 124
EarI CTCTTC 3 cut(s) 12, 26, 124
Ecl136II GAGCTC 2 cut(s) 133, 652
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 4 cut(s) 24, 135, 280, 654
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 2 cut(s) 133, 652
Eco57I CTGAAG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 273
EcoICRI GAGCTC 2 cut(s) 133, 652
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 4 cut(s) 24, 135, 280, 654
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 3 cut(s) 130, 440, 529
FaqI GGGAC 1 cut(s) 590
FatI CATG 3 cut(s) 126, 436, 525
FblI GTMKAC 2 cut(s) 71, 267
Fnu4HI GCNGC 4 cut(s) 333, 351, 741, 744
FokI GGATG 3 cut(s) 69, 326, 458
FriOI GRGCYC 4 cut(s) 24, 135, 280, 654
Fsp4HI GCNGC 4 cut(s) 333, 351, 741, 744
FspBI CTAG 2 cut(s) 368, 725
GlaI GCGC 2 cut(s) 97, 214
GluI GCNGC 4 cut(s) 333, 351, 741, 744
GsaI CCCAGC 1 cut(s) 145
HaeIII GGCC 3 cut(s) 140, 192, 278
HapII CCGG 5 cut(s) 105, 189, 261, 274, 494
HgaI GACGC 4 cut(s) 97, 206, 253, 278
HhaI GCGC 2 cut(s) 98, 215
Hin1I GRCGYC 4 cut(s) 108, 198, 264, 270
Hin1II CATG 3 cut(s) 130, 440, 529
Hin6I GCGC 2 cut(s) 96, 213
HinP1I GCGC 2 cut(s) 96, 213
HincII GTYRAC 2 cut(s) 72, 268
HindII GTYRAC 2 cut(s) 72, 268
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 8 cut(s) 47, 68, 164, 449, 460, 473, 499, 625
HpaII CCGG 5 cut(s) 105, 189, 261, 274, 494
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 72, 268
Hpy188I TCNGA 4 cut(s) 113, 388, 454, 649
Hpy188III TCNNGA 7 cut(s) 158, 253, 430, 470, 503, 617, 689
Hpy8I GTNNAC 2 cut(s) 72, 268
Hpy99I CGWCG 4 cut(s) 113, 269, 272, 690
HpyAV CCTTC 2 cut(s) 253, 295
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4V TGCA 4 cut(s) 332, 353, 488, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 239, 329, 463, 629
HpyF3I CTNAG 2 cut(s) 462, 646
Hsp92I GRCGYC 4 cut(s) 108, 198, 264, 270
Hsp92II CATG 3 cut(s) 130, 440, 529
HspAI GCGC 2 cut(s) 96, 213
KroI GCCGGC 2 cut(s) 104, 188
KroNI GCCGGC 2 cut(s) 106, 190
Kzo9I GATC 4 cut(s) 123, 225, 317, 517
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 113, 130
Lsp1109I GCAGC 4 cut(s) 337, 344, 752, 755
LweI GCATC 2 cut(s) 229, 475
MaeI CTAG 2 cut(s) 368, 725
MalI GATC 4 cut(s) 125, 227, 319, 519
MboI GATC 4 cut(s) 123, 225, 317, 517
MhlI GDGCHC 4 cut(s) 24, 135, 280, 654
MlyI GAGTC 4 cut(s) 77, 158, 454, 458
MmeI TCCRAC 2 cut(s) 91, 318
MnlI CCTC 7 cut(s) 27, 127, 194, 366, 385, 597, 655
MreI CGCCGGCG 1 cut(s) 104
MroNI GCCGGC 2 cut(s) 104, 188
MseI TTAA 2 cut(s) 309, 698
MspI CCGG 5 cut(s) 105, 189, 261, 274, 494
MspR9I CCNGG 3 cut(s) 53, 274, 275
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 2 cut(s) 96, 213
MwoI GCNNNNNNNGC 5 cut(s) 88, 239, 329, 463, 629
NaeI GCCGGC 2 cut(s) 106, 190
NciI CCSGG 2 cut(s) 274, 275
NdeII GATC 4 cut(s) 123, 225, 317, 517
NgoMIV GCCGGC 2 cut(s) 104, 188
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 3 cut(s) 130, 440, 529
NlaIV GGNNCC 2 cut(s) 115, 278
NmeAIII GCCGAG 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 554
PasI CCCWGGG 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 2 cut(s) 106, 190
PfeI GAWTC 4 cut(s) 47, 473, 499, 625
PkrI GCNGC 4 cut(s) 334, 352, 742, 745
PleI GAGTC 4 cut(s) 76, 158, 454, 457
PpsI GAGTC 4 cut(s) 76, 158, 454, 457
PpuMI RGGWCCY 1 cut(s) 181
Psp124BI GAGCTC 2 cut(s) 135, 654
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 115, 278
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 5 cut(s) 139, 181, 191, 276, 277
PspPPI RGGWCCY 1 cut(s) 181
RsaI GTAC 2 cut(s) 410, 660
RsaNI GTAC 2 cut(s) 409, 659
SacI GAGCTC 2 cut(s) 135, 654
SalI GTCGAC 2 cut(s) 70, 266
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 309, 698
SatI GCNGC 4 cut(s) 333, 351, 741, 744
Sau3AI GATC 4 cut(s) 123, 225, 317, 517
Sau96I GGNCC 5 cut(s) 139, 181, 191, 276, 277
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 4 cut(s) 77, 158, 454, 458
ScrFI CCNGG 3 cut(s) 53, 274, 275
SduI GDGCHC 4 cut(s) 24, 135, 280, 654
SfaNI GCATC 2 cut(s) 229, 475
SgrAI CRCCGGYG 1 cut(s) 104
SgrDI CGTCGACG 1 cut(s) 266
SinI GGWCC 1 cut(s) 181
SmaI CCCGGG 1 cut(s) 275
SmlI CTYRAG 3 cut(s) 134, 156, 293
SmoI CTYRAG 3 cut(s) 134, 156, 293
SrfI GCCCGGGC 1 cut(s) 275
SsiI CCGC 3 cut(s) 94, 211, 231
SspMI CTAG 2 cut(s) 368, 725
SstI GAGCTC 2 cut(s) 135, 654
StyD4I CCNGG 3 cut(s) 51, 272, 273
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 413
TaqI TCGA 3 cut(s) 71, 122, 267
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 4 cut(s) 47, 473, 499, 625
Tru1I TTAA 2 cut(s) 309, 698
Tru9I TTAA 2 cut(s) 309, 698
TseI GCWGC 4 cut(s) 332, 350, 740, 743
TspDTI ATGAA 2 cut(s) 600, 602
TspGWI ACGGA 3 cut(s) 80, 238, 730
TspMI CCCGGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 273
XmiI GTMKAC 2 cut(s) 71, 267
XspI CTAG 2 cut(s) 368, 725
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.