Rh2AG051400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006912
Physical Location & Seq
Forward (+)
4059710 .. 4061238
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG051400.1

Sequence Viewer

Length: 699 bp
ATGGTCTCTTCGCCGAATTCATCTCCCTCATCCTCTTCACCAACACCTGGTCAGGTCTCTTCGCAGCTTTGCGGAGTTGATTATGATCTCACTTTCTTGATCAACTCCTCCGCGCAGCCACAGCCCGCGGCTACGACGCCGTTTTACACCTCTCAATCACTGCCCTCGCCGTCTTCATACTTGTTCATCAGAATCGTCAGGGAGTGTGGACTCGCTGCGAACTGGAGCCCCTACATCAAGGCAGAGGAGGTCGGCGCAGGAGCTGGAGAATCCGGCATAGAAGAAGATGAGGTCGGCTTCGCGGACTTCGAATGTGGGGCGGAGGTCGGAGGATTAGGACGTAGAGGTGTACTTGAGCCAATGCCATCGCCGTACTTTAGCATTATGTATTGGAATACCCTTTGGAAATTAACCAACCTCGAAGAAGTCCTTCAGAAATTGAAACAAGGAACATTAACAACAGAAGAAAGACATACAATTTACCTCAAGCTCCAAAGTTTGGATTCCGAAAATGAGGTCTATAAGTTACTGCTGGATGGAGTTGCACCACAGGAAGAGGAAGATATGATTTGGAACCTGTATTCTGGAGCTGTTCTTGAGCACATATACTGGTGCATGGTGTGGACGCGATTCTACTTACCAGGGTATAGACGCTGCCCCATATGTGGCACACATCTAGCCAGGGTCCGGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

25.9

Weight (kDa)

4.74

Isoelectric Point (pI)

73.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 47, 499
AccII CGCG 4 cut(s) 113, 128, 302, 628
AciI CCGC 6 cut(s) 72, 111, 126, 128, 302, 320
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 351, 374
AfiI CCNNNNNNNGG 4 cut(s) 47, 499, 665, 687
AgsI TTSAA 1 cut(s) 442
AjnI CCWGG 3 cut(s) 46, 640, 680
AluBI AGCT 4 cut(s) 67, 263, 490, 590
AluI AGCT 4 cut(s) 67, 263, 490, 590
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 2 cut(s) 10, 61
AlwNI CAGNNNCTG 1 cut(s) 263
ApeKI GCWGC 4 cut(s) 64, 115, 215, 654
ApoI RAATTY 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
Asp700I GAANNNNTTC 1 cut(s) 429
AspLEI GCGC 2 cut(s) 115, 257
AspS9I GGNCC 1 cut(s) 685
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 1 cut(s) 30
AsuII TTCGAA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 685
BanII GRGCYC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 4 cut(s) 76, 127, 202, 641
BccI CCATC 2 cut(s) 373, 530
BceAI ACGGC 3 cut(s) 124, 154, 355
BciT130I CCWGG 3 cut(s) 48, 642, 682
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 10, 61
BfaI CTAG 1 cut(s) 677
BisI GCNGC 5 cut(s) 65, 116, 129, 216, 655
BlsI GCNGC 5 cut(s) 66, 117, 130, 217, 656
Bme1390I CCNGG 4 cut(s) 48, 642, 682, 689
Bme18I GGWCC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 685
BmiI GGNNCC 3 cut(s) 227, 575, 686
BmrFI CCNGG 4 cut(s) 48, 642, 682, 689
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 3 cut(s) 244, 285, 606
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 3 cut(s) 374, 470, 617
BpuMI CCSGG 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 10, 61
BsaJI CCNNGG 3 cut(s) 126, 641, 681
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 499, 665, 687
Bse1I ACTGG 2 cut(s) 227, 614
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 3 cut(s) 48, 642, 682
BseDI CCNNGG 3 cut(s) 126, 641, 681
BseGI GGATG 2 cut(s) 29, 541
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 4 cut(s) 47, 499, 665, 687
BseNI ACTGG 2 cut(s) 227, 614
BseRI GAGGAG 2 cut(s) 97, 260
BseXI GCAGC 4 cut(s) 76, 127, 202, 641
Bsh1236I CGCG 4 cut(s) 113, 128, 302, 628
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 2 cut(s) 273, 688
BslI CCNNNNNNNGG 4 cut(s) 47, 499, 665, 687
BsmAI GTCTC 2 cut(s) 10, 61
Bso31I GGTCTC 2 cut(s) 10, 61
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 230, 603
Bsp143I GATC 2 cut(s) 85, 99
BspACI CCGC 6 cut(s) 72, 111, 126, 128, 302, 320
BspFNI CGCG 4 cut(s) 113, 128, 302, 628
BspLI GGNNCC 3 cut(s) 227, 575, 686
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 2 cut(s) 10, 61
BsrI ACTGG 2 cut(s) 227, 614
BssECI CCNNGG 3 cut(s) 126, 641, 681
BssMI GATC 2 cut(s) 85, 99
BssNI GRCGYC 1 cut(s) 137
Bst2UI CCWGG 3 cut(s) 48, 642, 682
Bst6I CTCTTC 4 cut(s) 13, 40, 64, 549
BstACI GRCGYC 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 126
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 29, 541
BstFNI CGCG 4 cut(s) 113, 128, 302, 628
BstHHI GCGC 2 cut(s) 115, 257
BstKTI GATC 2 cut(s) 88, 102
BstMAI GTCTC 2 cut(s) 10, 61
BstMBI GATC 2 cut(s) 85, 99
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 3 cut(s) 48, 642, 682
BstSCI CCNGG 4 cut(s) 46, 640, 680, 687
BstUI CGCG 4 cut(s) 113, 128, 302, 628
BstV1I GCAGC 4 cut(s) 76, 127, 202, 641
BstV2I GAAGAC 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 29, 541
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 2 cut(s) 115, 257
Cfr13I GGNCC 1 cut(s) 685
Cfr42I CCGCGG 1 cut(s) 129
CseI GACGC 3 cut(s) 145, 634, 660
CsiI ACCWGGT 1 cut(s) 46
Csp6I GTAC 2 cut(s) 350, 373
CviAII CATG 1 cut(s) 616
CviQI GTAC 2 cut(s) 350, 373
DpnI GATC 2 cut(s) 87, 101
DpnII GATC 2 cut(s) 85, 99
Eam1104I CTCTTC 4 cut(s) 13, 40, 64, 549
EarI CTCTTC 4 cut(s) 13, 40, 64, 549
EciI GGCGGA 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 230
Eco31I GGTCTC 2 cut(s) 10, 61
Eco47I GGWCC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 46, 640, 680
EcoT38I GRGCYC 1 cut(s) 230
FaeI CATG 1 cut(s) 619
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 1 cut(s) 615
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 662
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 5 cut(s) 65, 116, 129, 216, 655
FokI GGATG 2 cut(s) 16, 548
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 65, 116, 129, 216, 655
FspBI CTAG 1 cut(s) 677
GlaI GCGC 2 cut(s) 114, 256
GluI GCNGC 5 cut(s) 65, 116, 129, 216, 655
GsuI CTGGAG 3 cut(s) 244, 285, 606
HapII CCGG 2 cut(s) 273, 688
HgaI GACGC 3 cut(s) 145, 634, 660
HhaI GCGC 2 cut(s) 115, 257
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 1 cut(s) 619
Hin6I GCGC 2 cut(s) 113, 255
HinP1I GCGC 2 cut(s) 113, 255
HinfI GANTC 5 cut(s) 192, 210, 269, 503, 630
HpaII CCGG 2 cut(s) 273, 688
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 3 cut(s) 209, 350, 624
Hpy188I TCNGA 4 cut(s) 191, 329, 435, 508
Hpy188III TCNNGA 3 cut(s) 97, 585, 596
Hpy8I GTNNAC 3 cut(s) 209, 350, 624
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 440
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 2 cut(s) 545, 615
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 1 cut(s) 619
HspAI GCGC 2 cut(s) 113, 255
Ksp22I TGATCA 1 cut(s) 99
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 2 cut(s) 85, 99
LmnI GCTCC 4 cut(s) 225, 260, 495, 587
Lsp1109I GCAGC 4 cut(s) 76, 127, 202, 641
MabI ACCWGGT 1 cut(s) 46
MaeI CTAG 1 cut(s) 677
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 525
MalI GATC 2 cut(s) 87, 101
MboI GATC 2 cut(s) 85, 99
MboII GAAGA 9 cut(s) 27, 51, 165, 293, 296, 434, 476, 566, 572
MhlI GDGCHC 2 cut(s) 230, 603
MluCI AATT 4 cut(s) 16, 407, 437, 477
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 2 cut(s) 410, 455
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 2 cut(s) 273, 688
MspR9I CCNGG 4 cut(s) 48, 642, 682, 689
MvaI CCWGG 3 cut(s) 48, 642, 682
MvnI CGCG 4 cut(s) 113, 128, 302, 628
MwoI GCNNNNNNNGC 1 cut(s) 121
NciI CCSGG 1 cut(s) 689
NdeI CATATG 1 cut(s) 662
NdeII GATC 2 cut(s) 85, 99
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 3 cut(s) 227, 575, 686
NspV TTCGAA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 4 cut(s) 192, 269, 503, 630
PflMI CCANNNNNTGG 2 cut(s) 47, 499
PfoI TCCNGGA 1 cut(s) 687
PkrI GCNGC 5 cut(s) 66, 117, 130, 217, 656
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 46, 640, 680
PspGI CCWGG 3 cut(s) 46, 640, 680
PspN4I GGNNCC 3 cut(s) 227, 575, 686
PspPI GGNCC 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 263
RsaI GTAC 2 cut(s) 351, 374
RsaNI GTAC 2 cut(s) 350, 373
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 2 cut(s) 410, 455
SatI GCNGC 5 cut(s) 65, 116, 129, 216, 655
Sau3AI GATC 2 cut(s) 85, 99
Sau96I GGNCC 1 cut(s) 685
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 4 cut(s) 48, 642, 682, 689
SduI GDGCHC 2 cut(s) 230, 603
SexAI ACCWGGT 1 cut(s) 46
Sfr303I CCGCGG 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 309
SgrBI CCGCGG 1 cut(s) 129
SinI GGWCC 1 cut(s) 685
SmlI CTYRAG 3 cut(s) 353, 485, 596
SmoI CTYRAG 3 cut(s) 353, 485, 596
Sse9I AATT 4 cut(s) 16, 407, 437, 477
SsiI CCGC 6 cut(s) 72, 111, 126, 128, 302, 320
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 46, 640, 680, 687
TaiI ACGT 1 cut(s) 343
TaqI TCGA 2 cut(s) 309, 420
TasI AATT 4 cut(s) 16, 407, 437, 477
TatI WGTACW 1 cut(s) 349
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 4 cut(s) 192, 269, 503, 630
Tru1I TTAA 2 cut(s) 410, 455
Tru9I TTAA 2 cut(s) 410, 455
TscAI CASTG 1 cut(s) 165
TseI GCWGC 4 cut(s) 64, 115, 215, 654
TspDTI ATGAA 3 cut(s) 9, 165, 175
TspRI CASTG 1 cut(s) 165
Van91I CCANNNNNTGG 2 cut(s) 47, 499
VpaK11BI GGWCC 1 cut(s) 685
XapI RAATTY 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.