Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0003929

Physical Location & Seq

Genomic Coordinates Reverse (-)

45534472 .. 45537132

Gene Structure 2661 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0358600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGAGTTTAGTTCTGATGAGAGCGCGTATAAAGCTTATGCTAAATATGGAGGAAAATCTGGTTTCAATGTGAGAAAACAACGAAAGAAGAGAAACAACAAGGGTTTGGTTGTAAAGTTGCTTTATTGTTGCTCAAAACAAGGTTACCGAAAGACCAGAACAAAGAGGGAAACATCTTATTCATTACCAGTTACAAGGGTCGGTTGTAAAGCCCATATGAGTTGCTATCGTCAAAGCAACGGAAAATTCCAGATTGTGTCGTTTGAGACAAATCACAATCATGAGTTGATTAAAACACCTATGAAGCATATGTTAAAGATTAATCGAAGTTTCTCTAAAGCTCAAAAAGAACATGCTGATGATGCTGAGATGTCAGGAATTTCAGCAAAAGCAACAGTTGAATTGATGAGTAGAGAAGTTGGGGGACGAGAGAATCTTGGTTTTATGGAAAAGGACTATCGGAATTACATACATCGAAAGCGAAGGGTGAAGATGGAAAAAGGAGATGCTGGAGCTATATTGCAATACTTCCAAAAAATGAAAGAGGATAATTCATCTTCTTTTTATTCAATGCAACTTGATGAGGATGATATGATCACGAATATTTTTTGGGCAGATGAGCGTTCAGTAAGTGATTATGGTCTTTTTGGGGACGTCGTTTGTTTTGACACAACTTATCAGACTAATGAATATGGTAGACCATTTGCTCCATTTGTTGGGGTCAATCATCATAAGCAAACAGTGGTATTTGGTGCAGCTTTATTATATGATGAAACCATTGAGTCCTTTAAGTGGCTTTTTGAGACTTTTCTTAGAGCGATGTCTGGAAAGCAACCAAAAACCATACTTACAGACCATTCTGCAGCAATGGCTAGTGCAATAGCAGAAGCTACAAAGAGGTTAAGTCGTGTGTTTCACGGATCAGAACAGTTTGCTCATGATTTTGGAAAGTGTGTATATGACTATGAGGATGAAGATGATTGGTTATTGGCATGGAGTAATATGCTCGAGAAACACAATTTGAAGGAGGATAAATGGTTAAAGAATTTGTTTGATTTGAGAAAAAAATGGGCATTGGTATATGGGCGGCATACTTTTACTGCAGATATGATGAGTACACAACGTAGTGAAAGTATGAATAATATTTTGAAAAAATACTTGAAGCCTAGTTATGACCTGTTGTGCTTCTTTGAACACTATGAGAGAGTTTTGGCTGATCGACGATATGAAGAATTAATTGCTGACTTCAAGATGATGCAAACCTCTCCTGTGTTATCTGCTAACGTAGAGATGTTGCAACATGCAGAGGAGGTGTATACCCCAGAAGCTTTTAGACTCTTCCAGCAACAATACACAAGTATTGGTGATTATGTTGCTAATAAAGTTAGCAAGTCTGAGATGAAATATGAATACAAAGTATCTTATCGTGGTGTTGCTCGAGAGCATTTGGTAAAATTTGATGCTTCAGAGCAAACAATAACTTGTAGTTGCATGAAGTTTAATTTTGTTGAGATTTTATGCCGTCATGCAATGAAAGTGTTGGATAGGAAGAACGTGAGAAGAATCCCTCCTACTTGCATATTAAATCGATGGAGTAAAGAAGCTAAGGCAAGAAGCATCACTTGTTATCATGGACCAAAGACTAATGAAAATCTCAAGCAGTCGATTGGAAAGCGGTACAGCCATTTGTGTCGTAATTATAGTGAGATTGCATCCTTTGCTGCAGAACATGAGGAATTAACAACGTATGCAGATGAATATGCCATCGAGTTGCTTAAAAATTTGGAAGAAAAGAAGAAGAAACTTTTGAAAGAAGATGCATGGGTGATTCAAACTTCAGATGTTGGAGCTTTGGAAGCAAGTAAAGCCGCAAAGAAACAACTATCCTTCCAGGATTCTATTCCTATTAGTGACAATTGCGAGCAACAAGAAGTCAATGAATATGAAGTTCCAATGCTTGATTTTGCAACTCAAGAATCCAATCTTAGCGCTGGTACCCAAGTATCAATTTATGGTACAAGTGTAACTTTGCAAAATGGCTTTTCTTTCACTCAAATGCTCCAGGAAATTTCAATGAATCATGCTAATTCTTCACAAAGTAATGAAGAAGGCAAAAAGCAGCTGGGAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

710

Amino Acids

82.15

Weight (kDa)

8.55

Isoelectric Point (pI)

44.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAR1	PF03101	13 - 97	1e-17	FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like	PF21056	179 - 296	2.6e-09	Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE	PF10551	219 - 296	6.2e-22	MULE transposase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003929)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59960 AT5G59960 AT5G59960
fragaria_vesca	FvH4_3g35470 FvH4_3g35470
malus_domestica	MD03G1097100.v1.1 MD11G1143700.v1.1
prunus_persica	Prupe.4G098100_v2.0.a1 Prupe.6G081500_v2.0.a1 Prupe.6G081500_v2.0.a1 Prupe.6G081500_v2.0.a1
pyrus_communis	pycom03g08010 pycom11g09330
rosa_chinensis	RchiOBHm_Chr1g0369801 RchiOBHm_Chr5g0063631
rosa_laevigata	RLG00000029314 RLG00000035658
rosa_multiflora	Rmu_sc0000368.1_g000040 Rmu_ssc0000309.1_g000060
rosa_roxburghii	Rroxscaffold_1G00017170 Rroxscaffold_2G00129250
rosa_rugosa	Rorug05G0358400 Rorug05G0358500 Rorug05G0358500 Rorug05G0358600
rosa_samantha	Rh2CG479400 Rh3DG316400 Rh5AG417200 Rh5BG432800 Rh5CG456000 Rh5DG446200 Rh6CG100700
rosa_wichuraiana	Rw3G025090 Rw5G039210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	657
Acc65I	GGTACC	1 cut(s)	1994
AccB1I	GGYRCC	1 cut(s)	1994
AccB7I	CCANNNNNTGG	1 cut(s)	716
AccI	GTMKAC	2 cut(s)	697, 1316
AccII	CGCG	1 cut(s)	26
AciI	CCGC	3 cut(s)	1087, 1675, 1869
AclWI	GGATC	1 cut(s)	928
AcsI	RAATTY	6 cut(s)	245, 378, 1045, 1454, 1780, 2067
AcuI	CTGAAG	2 cut(s)	1449, 1821
AcyI	GRCGYC	1 cut(s)	654
AfaI	GTAC	4 cut(s)	1117, 1679, 1996, 2017
AfeI	AGCGCT	1 cut(s)	1990
AfiI	CCNNNNNNNGG	2 cut(s)	716, 792
AjnI	CCWGG	2 cut(s)	1890, 2061
AluBI	AGCT	9 cut(s)	35, 341, 515, 758, 890, 1328, 1604, 1850, 2122
AluI	AGCT	9 cut(s)	35, 341, 515, 758, 890, 1328, 1604, 1850, 2122
Alw26I	GTCTC	2 cut(s)	260, 797
AlwI	GGATC	1 cut(s)	928
Ama87I	CYCGRG	2 cut(s)	1007, 1437
Aor51HI	AGCGCT	1 cut(s)	1990
ApeKI	GCWGC	4 cut(s)	755, 863, 1721, 2119
ApoI	RAATTY	6 cut(s)	245, 378, 1045, 1454, 1780, 2067
AseI	ATTAAT	2 cut(s)	321, 1235
Asp718I	GGTACC	1 cut(s)	1994
AspLEI	GCGC	2 cut(s)	26, 1991
AspS9I	GGNCC	1 cut(s)	1634
AsuHPI	GGTGA	3 cut(s)	499, 1376, 1837
AvaI	CYCGRG	2 cut(s)	1007, 1437
AvaII	GGWCC	1 cut(s)	1634
BaeI	ACNNNNGTAYC	6 cut(s)	1986, 1986, 2007, 2019, 2019, 2040
BanI	GGYRCC	1 cut(s)	1994
BbvI	GCAGC	3 cut(s)	767, 875, 1708
BccI	CCATC	3 cut(s)	487, 1584, 1772
BceAI	ACGGC	1 cut(s)	1506
BciT130I	CCWGG	2 cut(s)	1892, 2063
BclI	TGATCA	1 cut(s)	594
BcoDI	GTCTC	2 cut(s)	260, 797
BfaI	CTAG	2 cut(s)	873, 1167
BfmI	CTRYAG	3 cut(s)	861, 1101, 1722
BfoI	RGCGCY	1 cut(s)	1992
BisI	GCNGC	6 cut(s)	756, 864, 1088, 1722, 1869, 2120
BlsI	GCNGC	6 cut(s)	757, 865, 1089, 1723, 1870, 2121
Bme1390I	CCNGG	2 cut(s)	1892, 2063
Bme18I	GGWCC	1 cut(s)	1634
BmeT110I	CYCGRG	2 cut(s)	1007, 1437
BmgT120I	GGNCC	1 cut(s)	1634
BmiI	GGNNCC	1 cut(s)	1996
BmrFI	CCNGG	2 cut(s)	1892, 2063
BmsI	GCATC	7 cut(s)	352, 496, 1245, 1450, 1626, 1721, 1807
BpmI	CTGGAG	2 cut(s)	531, 2045
Bpu10I	CCTNAGC	1 cut(s)	1605
BpuEI	CTTGAG	2 cut(s)	1640, 1956
Bsa29I	ATCGAT	1 cut(s)	1588
BsaHI	GRCGYC	1 cut(s)	654
Bsc4I	CCNNNNNNNGG	2 cut(s)	716, 792
Bse1I	ACTGG	1 cut(s)	188
Bse3DI	GCAATG	2 cut(s)	873, 1536
BseBI	CCWGG	2 cut(s)	1892, 2063
BseCI	ATCGAT	1 cut(s)	1588
BseGI	GGATG	3 cut(s)	592, 976, 1712
BseLI	CCNNNNNNNGG	2 cut(s)	716, 792
BseMI	GCAATG	2 cut(s)	873, 1536
BseMII	CTCAG	2 cut(s)	357, 1386
BseNI	ACTGG	1 cut(s)	188
BseRI	GAGGAG	1 cut(s)	1322
BseXI	GCAGC	3 cut(s)	767, 875, 1708
BseYI	CCCAGC	1 cut(s)	2122
BsgI	GTGCAG	1 cut(s)	774
Bsh1236I	CGCG	1 cut(s)	26
BshNI	GGYRCC	1 cut(s)	1994
BshVI	ATCGAT	1 cut(s)	1588
BsiHKCI	CYCGRG	2 cut(s)	1007, 1437
BslFI	GGGAC	2 cut(s)	438, 665
BslI	CCNNNNNNNGG	2 cut(s)	716, 792
BsmAI	GTCTC	2 cut(s)	260, 797
BsmFI	GGGAC	2 cut(s)	438, 665
BsoBI	CYCGRG	2 cut(s)	1007, 1437
Bsp143I	GATC	3 cut(s)	594, 920, 1216
BspACI	CCGC	3 cut(s)	1087, 1675, 1869
BspCNI	CTCAG	2 cut(s)	358, 1387
BspDI	ATCGAT	1 cut(s)	1588
BspFNI	CGCG	1 cut(s)	26
BspHI	TCATGA	2 cut(s)	280, 937
BspLI	GGNNCC	1 cut(s)	1996
BspMAI	CTGCAG	3 cut(s)	865, 1105, 1726
BspPI	GGATC	1 cut(s)	928
BspT107I	GGYRCC	1 cut(s)	1994
BsrDI	GCAATG	2 cut(s)	873, 1536
BsrI	ACTGG	1 cut(s)	188
BssMI	GATC	3 cut(s)	594, 920, 1216
BssNAI	GTATAC	1 cut(s)	1317
BssNI	GRCGYC	1 cut(s)	654
Bst1107I	GTATAC	1 cut(s)	1317
Bst2UI	CCWGG	2 cut(s)	1892, 2063
Bst4CI	ACNGT	3 cut(s)	397, 742, 930
Bst6I	CTCTTC	2 cut(s)	83, 1343
BstACI	GRCGYC	1 cut(s)	654
BstAPI	GCANNNNNTGC	1 cut(s)	1718
BstC8I	GCNNGC	1 cut(s)	1922
BstDEI	CTNAG	5 cut(s)	366, 812, 1395, 1605, 1985
BstEII	GGTNACC	1 cut(s)	143
BstF5I	GGATG	3 cut(s)	592, 976, 1712
BstFNI	CGCG	1 cut(s)	26
BstH2I	RGCGCY	1 cut(s)	1992
BstHHI	GCGC	2 cut(s)	26, 1991
BstKTI	GATC	3 cut(s)	597, 923, 1219
BstMAI	GTCTC	2 cut(s)	260, 797
BstMBI	GATC	3 cut(s)	594, 920, 1216
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 362, 869, 1718, 1856, 1865
BstNI	CCWGG	2 cut(s)	1892, 2063
BstNSI	RCATGY	2 cut(s)	356, 1304
BstPI	GGTNACC	1 cut(s)	143
BstSCI	CCNGG	2 cut(s)	1890, 2061
BstSFI	CTRYAG	3 cut(s)	861, 1101, 1722
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	3 cut(s)	767, 875, 1708
BstZ17I	GTATAC	1 cut(s)	1317
Bsu15I	ATCGAT	1 cut(s)	1588
BsuTUI	ATCGAT	1 cut(s)	1588
BtgZI	GCGATG	1 cut(s)	833
BtsCI	GGATG	3 cut(s)	592, 976, 1712
BtsIMutI	CAGTG	1 cut(s)	747
Cac8I	GCNNGC	1 cut(s)	1922
CciI	TCATGA	2 cut(s)	280, 937
CfoI	GCGC	2 cut(s)	26, 1991
Cfr13I	GGNCC	1 cut(s)	1634
ClaI	ATCGAT	1 cut(s)	1588
Csp6I	GTAC	4 cut(s)	1116, 1678, 1995, 2016
CviQI	GTAC	4 cut(s)	1116, 1678, 1995, 2016
DdeI	CTNAG	5 cut(s)	366, 812, 1395, 1605, 1985
DpnI	GATC	3 cut(s)	596, 922, 1218
DpnII	GATC	3 cut(s)	594, 920, 1216
Eam1104I	CTCTTC	2 cut(s)	83, 1343
EarI	CTCTTC	2 cut(s)	83, 1343
Eco47I	GGWCC	1 cut(s)	1634
Eco47III	AGCGCT	1 cut(s)	1990
Eco57I	CTGAAG	2 cut(s)	1449, 1821
Eco88I	CYCGRG	2 cut(s)	1007, 1437
Eco91I	GGTNACC	1 cut(s)	143
EcoO65I	GGTNACC	1 cut(s)	143
EcoRII	CCWGG	2 cut(s)	1890, 2061
EcoT22I	ATGCAT	1 cut(s)	1822
FalI	AAGNNNNNCTT	4 cut(s)	1606, 1638, 2011, 2043
FaqI	GGGAC	2 cut(s)	438, 665
FauNDI	CATATG	2 cut(s)	216, 309
FbaI	TGATCA	1 cut(s)	594
FblI	GTMKAC	2 cut(s)	697, 1316
Fnu4HI	GCNGC	6 cut(s)	756, 864, 1088, 1722, 1869, 2120
FokI	GGATG	3 cut(s)	599, 983, 1699
Fsp4HI	GCNGC	6 cut(s)	756, 864, 1088, 1722, 1869, 2120
FspBI	CTAG	2 cut(s)	873, 1167
GlaI	GCGC	2 cut(s)	25, 1990
GluI	GCNGC	6 cut(s)	756, 864, 1088, 1722, 1869, 2120
GsaI	CCCAGC	1 cut(s)	2126
GsuI	CTGGAG	2 cut(s)	531, 2045
HaeII	RGCGCY	1 cut(s)	1992
HhaI	GCGC	2 cut(s)	26, 1991
Hin1I	GRCGYC	1 cut(s)	654
Hin6I	GCGC	2 cut(s)	24, 1989
HinP1I	GCGC	2 cut(s)	24, 1989
HindIII	AAGCTT	2 cut(s)	33, 1326
HinfI	GANTC	8 cut(s)	433, 782, 1335, 1563, 1828, 1895, 1976, 2077
HphI	GGTGA	3 cut(s)	499, 1376, 1837
Hpy166II	GTNNAC	3 cut(s)	698, 1118, 1317
Hpy188I	TCNGA	7 cut(s)	16, 462, 681, 925, 1396, 1468, 1840
Hpy8I	GTNNAC	3 cut(s)	698, 1118, 1317
Hpy99I	CGWCG	2 cut(s)	659, 1224
HpyAV	CCTTC	4 cut(s)	477, 1018, 1897, 2102
HpyCH4III	ACNGT	3 cut(s)	397, 742, 930
HpyCH4IV	ACGT	5 cut(s)	654, 1123, 1284, 1554, 1745
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 362, 869, 1718, 1856, 1865
HpyF3I	CTNAG	5 cut(s)	366, 812, 1395, 1605, 1985
HpySE526I	ACGT	5 cut(s)	654, 1123, 1284, 1554, 1745
Hsp92I	GRCGYC	1 cut(s)	654
HspAI	GCGC	2 cut(s)	24, 1989
KpnI	GGTACC	1 cut(s)	1998
Ksp22I	TGATCA	1 cut(s)	594
Kzo9I	GATC	3 cut(s)	594, 920, 1216
LmnI	GCTCC	4 cut(s)	512, 712, 1847, 2064
Lsp1109I	GCAGC	3 cut(s)	767, 875, 1708
LweI	GCATC	7 cut(s)	352, 496, 1245, 1450, 1626, 1721, 1807
MaeI	CTAG	2 cut(s)	873, 1167
MaeII	ACGT	5 cut(s)	654, 1123, 1284, 1554, 1745
MaeIII	GTNAC	4 cut(s)	143, 190, 1910, 2023
MalI	GATC	3 cut(s)	596, 922, 1218
MboI	GATC	3 cut(s)	594, 920, 1216
MfeI	CAATTG	1 cut(s)	1915
MlyI	GAGTC	2 cut(s)	791, 1329
MmeI	TCCRAC	2 cut(s)	1521, 1825
Mph1103I	ATGCAT	1 cut(s)	1822
MslI	CAYNNNNRTG	3 cut(s)	279, 357, 2054
MspA1I	CMGCKG	1 cut(s)	2122
MspR9I	CCNGG	2 cut(s)	1892, 2063
MunI	CAATTG	1 cut(s)	1915
MvaI	CCWGG	2 cut(s)	1892, 2063
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	6 cut(s)	32, 362, 869, 1718, 1856, 1865
NdeI	CATATG	2 cut(s)	216, 309
NdeII	GATC	3 cut(s)	594, 920, 1216
NlaIV	GGNNCC	1 cut(s)	1996
NmuCI	GTSAC	1 cut(s)	1910
NsiI	ATGCAT	1 cut(s)	1822
NspI	RCATGY	2 cut(s)	356, 1304
PaeR7I	CTCGAG	2 cut(s)	1007, 1437
PagI	TCATGA	2 cut(s)	280, 937
PfeI	GAWTC	6 cut(s)	433, 1563, 1828, 1895, 1976, 2077
PflMI	CCANNNNNTGG	1 cut(s)	716
PfoI	TCCNGGA	2 cut(s)	1890, 2061
PkrI	GCNGC	6 cut(s)	757, 865, 1089, 1723, 1870, 2121
PleI	GAGTC	2 cut(s)	790, 1329
PpsI	GAGTC	2 cut(s)	790, 1329
PshBI	ATTAAT	2 cut(s)	321, 1235
Psp6I	CCWGG	2 cut(s)	1890, 2061
PspEI	GGTNACC	1 cut(s)	143
PspFI	CCCAGC	1 cut(s)	2122
PspGI	CCWGG	2 cut(s)	1890, 2061
PspN4I	GGNNCC	1 cut(s)	1996
PspPI	GGNCC	1 cut(s)	1634
PstI	CTGCAG	3 cut(s)	865, 1105, 1726
PvuII	CAGCTG	1 cut(s)	2122
RsaI	GTAC	4 cut(s)	1117, 1679, 1996, 2017
RsaNI	GTAC	4 cut(s)	1116, 1678, 1995, 2016
RseI	CAYNNNNRTG	3 cut(s)	279, 357, 2054
SatI	GCNGC	6 cut(s)	756, 864, 1088, 1722, 1869, 2120
Sau3AI	GATC	3 cut(s)	594, 920, 1216
Sau96I	GGNCC	1 cut(s)	1634
SchI	GAGTC	2 cut(s)	791, 1329
ScrFI	CCNGG	2 cut(s)	1892, 2063
SfaNI	GCATC	7 cut(s)	352, 496, 1245, 1450, 1626, 1721, 1807
SfcI	CTRYAG	3 cut(s)	861, 1101, 1722
Sfr274I	CTCGAG	2 cut(s)	1007, 1437
SinI	GGWCC	1 cut(s)	1634
SlaI	CTCGAG	2 cut(s)	1007, 1437
SmiMI	CAYNNNNRTG	3 cut(s)	279, 357, 2054
SmlI	CTYRAG	4 cut(s)	1007, 1437, 1655, 1971
SmoI	CTYRAG	4 cut(s)	1007, 1437, 1655, 1971
SsiI	CCGC	3 cut(s)	1087, 1675, 1869
SspI	AATATT	2 cut(s)	604, 1144
SspMI	CTAG	2 cut(s)	873, 1167
StyD4I	CCNGG	2 cut(s)	1890, 2061
TaaI	ACNGT	3 cut(s)	397, 742, 930
TaiI	ACGT	5 cut(s)	657, 1126, 1287, 1557, 1748
TaqI	TCGA	8 cut(s)	325, 475, 1008, 1219, 1438, 1588, 1664, 1767
TatI	WGTACW	1 cut(s)	1115
TauI	GCSGC	2 cut(s)	1090, 1871
TfiI	GAWTC	6 cut(s)	433, 1563, 1828, 1895, 1976, 2077
TscAI	CASTG	1 cut(s)	747
TseFI	GTSAC	1 cut(s)	1910
TseI	GCWGC	4 cut(s)	755, 863, 1721, 2119
Tsp45I	GTSAC	1 cut(s)	1910
TspGWI	ACGGA	2 cut(s)	255, 933
TspRI	CASTG	1 cut(s)	747
Van91I	CCANNNNNTGG	1 cut(s)	716
VpaK11BI	GGWCC	1 cut(s)	1634
VspI	ATTAAT	2 cut(s)	321, 1235
XapI	RAATTY	6 cut(s)	245, 378, 1045, 1454, 1780, 2067
XceI	RCATGY	2 cut(s)	356, 1304
XhoI	CTCGAG	2 cut(s)	1007, 1437
XmiI	GTMKAC	2 cut(s)	697, 1316
XspI	CTAG	2 cut(s)	873, 1167
ZraI	GACGTC	1 cut(s)	655
Zsp2I	ATGCAT	1 cut(s)	1822

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.