Rorug05G0394900

ABC transporter A family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005930
Physical Location & Seq
Reverse (-)
54178962 .. 54191386
12425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0394900.1

Sequence Viewer

Length: 3066 bp
ATGGCTTTGTCCGGCATGCGAGGTCTCTCTGTTTTCATCAGCGACATTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTCCGCGTCGATAAGGAGCTCGGGAATGTTCGTACGCGCTTCAAAAACGAAAAGGGTTTGACACCGTATGAGAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGTTGGGATATGATGTGGATTTTGGTCACATGGAAGCAGTCTCTCTTATATCAGCGCCCAAGTATCCAGAAAAGCAGGTTGGGTACATTGTGACATCATGTCTACTCAATGAAAACCACGATTTTCTGAGATTAGCGATCAATACTGTACGGAATGATATTATTGGTCGTAATGAAACTTTCCAATGTTTGGCATTGACTATGGTTGGGAATATTGGAGGCAGAGAATTTGCGGAGTCATTAGCACCTGATGTTCAAAAGTTGCTTATTTCTAGCAGCTACAGACCGCTTGTGAGAAAAAAAGCTGCACTATGTCTTTTGCGCTTGTATAGGAAAAATCCTGATGTTGTCAATATAGATGGCTGGGCCGATCGCATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTGACATCATCTATGAGCCTTCTTGTTGCTCTTGTATCAAATCACCACGATGCTTACTGGAGTTGTCTTCCAAAATGTGTTAAAATACTAGAACGGCTTGCGAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGCATCCCATCTCCCTGGCTCCAGGTCAAGACAATGAGGGTTCTGCAGTATTTTCCCACTGTTGAAGATCCAAATACAAGAAGATCATTGTTTGAGGTCTTACAACGTATATTGATGGGTACGGATGTTGTGAAAAATGTTAATAAGAACAACGCATCGCACGCTGTTCTTTTTGAAGCCCTCGCACTTGTCATGCATCTTGATGCTGAGAAGGAGATGATGTCCCAATGTGTTGCACTTCTGGGAAAATTTATTGCTGTGCGAGAACCAAATATTCGATATCTTGGTTTGGAAAATATGACCCGCATGCTAATGGTAACGGATGTGCAAGATATCATCAAGAGACATCAAGCCCAGATTATCACCTCATTGAAGGATCCAGATGTCAGCATTCGTAGGCGTGCTCTTGACCTGCTTTATGGCATGTGTGATGTTTCAAATGCAAAAGACATAGTTGAAGAATTACTGCAGTATCTTAGTACAGCAGACTTCGCAATGCGCGAGGAATTGTCACTTAAGGCTGCCATTCTTGCAGAAAAATTTGCTCCTGATCTATCATGGTATGTAGATGTGATTCTTCAATTGATTGATAAGGCAGGAGATTTTGTTAGTGATGATATATGGTTCCGTGTTGTGCAATTTGTTACAAATAATGAAGACTTACAGCCTTATGCTGCAGCAAAAGCCAGGGAGTATCTTGACAAACCTGCTATACATGAGACAATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGTCACCTTTTAGCCAGACGGCCTGGGTGTAGTCCAAAAGAATTATTTGCCGTCATACATGAGAAGCTTCCTACTGTATCCACTTCTACAATACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAAGAACTACAGAATCAGATTTGGGCAATATTCAATAAATACGAGAGTTGCATCGATGTTGAGATACAACAACGAGCTGCTGAATACCTTGCCTTAAGTAGGAGAGGTGAAGCTCTTGTTGATATATTGGCTGAAATGCCTAAATTCCCTGAGCGGCAGTCTGCATTGATCAAAAAGGCTGAAGATACTGAAGTTGATACCGCAGAACAAAGTGCTATCAAGTTGCGCGCCCAGCAGCAAACATCAAATGCTTTGGTTGTGACCGACCAACGTCCCGCTAATGGTACCCCACCTGCCAACCACCAGCTTGGTCTTGTGAAGATACCCAACATGAGCAGTAATGTGGATCACAATTCAACCGATCAAGGGTTGTCTCAGGAAAATGGTACTTTGAATAAAGTTGATCCTCAAACTCCTTCACCGGATCTCCTTGGTGACCTCTTGGGTCCGCTGGCAATTGAAGGCCCTCCGGGTACTGCTGTTCAATCACATCAAACTGCAGTCCCTGGATCTGGTGCTGATCCAAATGCAGTGGATGCTACGGCCATAGTTCCTGTTGGCGATGAGCCAAGTTCTGTTCAGCCTATAGGAAATATTGCAGAAAGATTTCATGCCTTGTGCTTGAAAGATAGTGGTGTTCTGTATGAAGATCCTAATATTCAGATCGGAATTAAAGCAGAGTGGCGCTTACATCAGGGTTGTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGTATCAGTTCAGGCTGTAATAATGCCTCCCTCCCACTTTAAAATGGAGCTCTCACTAGTGCCAGAGACTATTCCTCCTAGGGCACAGGTACAGTGCCCCCTTGAAGTTGTGAATCTCCGCCCAAGTAGGGATGTAGCAGTTCTCGACTTCTCTTACAAGTTTGGTCACAATATGGTCAATGTCAAGCTTCGTCTCCCAGCTGTCTTGAATAAATTTCTTCAACCTATACCAGTGTCTGCTGAAGAGTTCTTTCCTCAGTGGAGATCACTCTCAGGACCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGCGTCAAACCACTGCCACTTGCAGAAATGGCAAATCTAATTAACAGCTTCCGGTTGATGGTTTGTCCAGGACTTGACCCTAATCCAAATAATCTTGTTGCAAGCACAACCTTTTATTCAGAAAGTACACGGGCGATGCTTTGCTTGGCTAGAATAGAAACAGATCCAGCAGACAGAACCCAGTTGCGTATGACGGTTGCTTCAGGGGATCCTACGTTAACATTTGAGTTGAAGGAGTTCATCAAGGAACAAATAGTTAACATCCCTGTAGCTCCTCGTGCTCCTGGACCAGTGCCACCAGCACCTCCAGTAGCTCAACCGACCAGTCCAGCTGCAGCATTAACGGATCCTGGTGCTCTGCTGGCCGGTTTGCTGTGA

Protein Analysis

1021

Amino Acids

113.69

Weight (kDa)

6.0

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 2e-116 Adaptin N terminal region
Cnd1 PF12717 125 - 263 1.1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 758 - 854 1.9e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 866 - 975 9.6e-16 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1942
Acc36I ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Acc65I GGTACC 1 cut(s) 1925
AccB1I GGYRCC 1 cut(s) 1925
AccB7I CCANNNNNTGG 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 1795
AccI GTMKAC 1 cut(s) 289
AccII CGCG 4 cut(s) 84, 115, 1236, 1869
AciI CCGC 9 cut(s) 82, 419, 473, 1039, 1795, 1842, 1917, 2090, 2500
AcoI YGGCCR 2 cut(s) 2184, 3051
AcsI RAATTY 6 cut(s) 413, 983, 1274, 1490, 1784, 2594
AcuI CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
AcyI GRCGYC 1 cut(s) 2691
AfiI CCNNNNNNNGG 7 cut(s) 376, 1740, 1922, 2007, 2153, 2509, 2746
AflII CTTAAG 2 cut(s) 1250, 1735
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 2437
AjnI CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
Alw21I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
Alw26I GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
AlwNI CAGNNNCTG 2 cut(s) 2147, 2618
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 5 cut(s) 552, 1514, 2104, 2184, 3051
ApeKI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
ApoI RAATTY 6 cut(s) 413, 983, 1274, 1490, 1784, 2594
Asp700I GAANNNNTTC 2 cut(s) 2247, 2924
Asp718I GGTACC 1 cut(s) 1925
AspA2I CCTAGG 1 cut(s) 2459
AspLEI GCGC 7 cut(s) 117, 244, 510, 1236, 1869, 1871, 2328
AspS9I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
AsuC2I CCSGG 1 cut(s) 2112
AsuHPI GGTGA 7 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2367
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 3 cut(s) 2087, 2657, 2975
AvrII CCTAGG 1 cut(s) 2459
BaeGI GKGCMC 2 cut(s) 2467, 2480
BaeI ACNNNNGTAYC 8 cut(s) 846, 879, 1190, 1223, 1818, 1851, 2106, 2139
BamHI GGATCC 3 cut(s) 1111, 2896, 3034
BanI GGYRCC 1 cut(s) 1925
BanII GRGCYC 2 cut(s) 99, 2433
BauI CACGAG 1 cut(s) 2964
BbsI GAAGAC 2 cut(s) 656, 1398
Bbv12I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BbvI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BccI CCATC 4 cut(s) 539, 751, 844, 2740
BceAI ACGGC 4 cut(s) 707, 1529, 1529, 2199
BciT130I CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BciVI GTATCC 2 cut(s) 261, 1582
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 1 cut(s) 2112
BcoDI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BcuI ACTAGT 1 cut(s) 2437
BfaI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
BfmI CTRYAG 9 cut(s) 466, 779, 1202, 1410, 1649, 2139, 2226, 2955, 3021
BfoI RGCGCY 2 cut(s) 245, 2329
BfrI CTTAAG 2 cut(s) 1250, 1735
BfuAI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BfuI GTATCC 2 cut(s) 261, 1582
BglII AGATCT 1 cut(s) 1614
BlnI CCTAGG 1 cut(s) 2459
Bme18I GGWCC 3 cut(s) 2087, 2657, 2975
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
BmiI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BmrI ACTGGG 1 cut(s) 2863
BmsI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
BmuI ACTGGG 1 cut(s) 2863
BoxI GACNNNNGTC 1 cut(s) 1911
BpiI GAAGAC 2 cut(s) 656, 1398
BpmI CTGGAG 4 cut(s) 676, 740, 1620, 2979
Bpu10I CCTNAGC 1 cut(s) 1791
BpuMI CCSGG 1 cut(s) 2112
Bsa29I ATCGAT 1 cut(s) 1695
BsaHI GRCGYC 1 cut(s) 2691
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BsaWI WCCGGW 2 cut(s) 2062, 2739
Bsc4I CCNNNNNNNGG 7 cut(s) 376, 1740, 1922, 2007, 2153, 2509, 2746
Bse118I RCCGGY 1 cut(s) 3053
Bse1I ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
Bse3DI GCAATG 1 cut(s) 1236
BseBI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BseCI ATCGAT 1 cut(s) 1695
BseDI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BseGI GGATG 6 cut(s) 738, 865, 1063, 2182, 2518, 2949
BseLI CCNNNNNNNGG 7 cut(s) 376, 1740, 1922, 2007, 2153, 2509, 2746
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 6 cut(s) 305, 933, 1782, 2030, 2651, 2667
BseNI ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
BsePI GCGCGC 1 cut(s) 1867
BseRI GAGGAG 1 cut(s) 2952
BseSI GKGCMC 2 cut(s) 2467, 2480
BseXI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BseYI CCCAGC 3 cut(s) 549, 1872, 2578
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 4 cut(s) 84, 115, 1236, 1869
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 5 cut(s) 554, 1516, 2106, 2186, 3053
BshNI GGYRCC 1 cut(s) 1925
BshVI ATCGAT 1 cut(s) 1695
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
BsiWI CGTACG 1 cut(s) 110
BslFI GGGAC 3 cut(s) 943, 1899, 2129
BslI CCNNNNNNNGG 7 cut(s) 376, 1740, 1922, 2007, 2153, 2509, 2746
BsmAI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BsmBI CGTCTC 1 cut(s) 2579
BsmFI GGGAC 3 cut(s) 943, 1899, 2129
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 5 cut(s) 554, 1516, 2106, 2186, 3053
Bso31I GGTCTC 1 cut(s) 29
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
BspACI CCGC 9 cut(s) 82, 419, 473, 1039, 1795, 1842, 1917, 2090, 2500
BspANI GGCC 5 cut(s) 554, 1516, 2106, 2186, 3053
BspCNI CTCAG 6 cut(s) 306, 934, 1783, 2029, 2650, 2666
BspDI ATCGAT 1 cut(s) 1695
BspFNI CGCG 4 cut(s) 84, 115, 1236, 1869
BspLI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BspMAI CTGCAG 5 cut(s) 783, 1206, 1414, 2143, 3025
BspMI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BspT107I GGYRCC 1 cut(s) 1925
BspTI CTTAAG 2 cut(s) 1250, 1735
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 1795
BsrDI GCAATG 1 cut(s) 1236
BsrFI RCCGGY 1 cut(s) 3053
BsrI ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
BssAI RCCGGY 1 cut(s) 3053
BssECI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BssHII GCGCGC 1 cut(s) 1867
BssNI GRCGYC 1 cut(s) 2691
BssSI CACGAG 1 cut(s) 2964
BssT1I CCWWGG 2 cut(s) 2071, 2459
Bst2BI CACGAG 1 cut(s) 2964
Bst2UI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
Bst4CI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
Bst6I CTCTTC 1 cut(s) 2619
BstACI GRCGYC 1 cut(s) 2691
BstAFI CTTAAG 2 cut(s) 1250, 1735
BstAPI GCANNNNNTGC 1 cut(s) 2177
BstC8I GCNNGC 9 cut(s) 17, 696, 897, 1043, 1137, 1869, 2094, 2791, 3051
BstDEI CTNAG 8 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637, 2653
BstEII GGTNACC 2 cut(s) 1496, 2075
BstENI CCTNNNNNAGG 1 cut(s) 1738
BstF5I GGATG 6 cut(s) 738, 865, 1063, 2182, 2518, 2949
BstFNI CGCG 4 cut(s) 84, 115, 1236, 1869
BstH2I RGCGCY 2 cut(s) 245, 2329
BstHHI GCGC 7 cut(s) 117, 244, 510, 1236, 1869, 1871, 2328
BstMAI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BstMCI CGRYCG 1 cut(s) 559
BstNI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BstNSI RCATGY 3 cut(s) 19, 1045, 1162
BstPAI GACNNNNGTC 1 cut(s) 1911
BstPI GGTNACC 2 cut(s) 1496, 2075
BstSFI CTRYAG 9 cut(s) 466, 779, 1202, 1410, 1649, 2139, 2226, 2955, 3021
BstSLI GKGCMC 2 cut(s) 2467, 2480
BstUI CGCG 4 cut(s) 84, 115, 1236, 1869
BstV1I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BstV2I GAAGAC 2 cut(s) 656, 1398
BstX2I RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
BstXI CCANNNNNNTGG 2 cut(s) 750, 1949
BstYI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
Bsu15I ATCGAT 1 cut(s) 1695
BsuI GTATCC 2 cut(s) 261, 1582
BsuRI GGCC 5 cut(s) 554, 1516, 2106, 2186, 3053
BsuTUI ATCGAT 1 cut(s) 1695
BtgZI GCGATG 3 cut(s) 876, 2217, 2837
BtsCI GGATG 6 cut(s) 738, 865, 1063, 2182, 2518, 2949
BtsI GCAGTG 2 cut(s) 2178, 2699
BtsIMutI CAGTG 8 cut(s) 792, 1477, 2178, 2480, 2619, 2645, 2699, 2985
BveI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Cac8I GCNNGC 9 cut(s) 17, 696, 897, 1043, 1137, 1869, 2094, 2791, 3051
CaiI CAGNNNCTG 2 cut(s) 2147, 2618
CfoI GCGC 7 cut(s) 117, 244, 510, 1236, 1869, 1871, 2328
Cfr10I RCCGGY 1 cut(s) 3053
Cfr13I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
ClaI ATCGAT 1 cut(s) 1695
CseI GACGC 2 cut(s) 73, 2680
CspCI CAANNNNNGTGG 2 cut(s) 2154, 2189
DdeI CTNAG 8 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637, 2653
DraI TTTAAA 1 cut(s) 2422
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 2 cut(s) 2184, 3051
Eam1104I CTCTTC 1 cut(s) 2619
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 2619
EciI GGCGGA 1 cut(s) 2489
Ecl136II GAGCTC 2 cut(s) 97, 2431
Eco130I CCWWGG 2 cut(s) 2071, 2459
Eco24I GRGCYC 2 cut(s) 99, 2433
Eco31I GGTCTC 1 cut(s) 29
Eco32I GATATC 2 cut(s) 1016, 1069
Eco47I GGWCC 3 cut(s) 2087, 2657, 2975
Eco53kI GAGCTC 2 cut(s) 97, 2431
Eco57I CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 2 cut(s) 1496, 2075
EcoICRI GAGCTC 2 cut(s) 97, 2431
EcoNI CCTNNNNNAGG 1 cut(s) 1738
EcoO109I RGGNCCY 1 cut(s) 2105
EcoO65I GGTNACC 2 cut(s) 1496, 2075
EcoRII CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
EcoRV GATATC 2 cut(s) 1016, 1069
EcoT14I CCWWGG 2 cut(s) 2071, 2459
EcoT22I ATGCAT 1 cut(s) 933
EcoT38I GRGCYC 2 cut(s) 99, 2433
ErhI CCWWGG 2 cut(s) 2071, 2459
Esp3I CGTCTC 1 cut(s) 2579
FaqI GGGAC 3 cut(s) 943, 1899, 2129
FauI CCCGC 2 cut(s) 1046, 1924
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 289
FokI GGATG 6 cut(s) 725, 872, 1070, 2189, 2525, 2936
FriOI GRGCYC 2 cut(s) 99, 2433
FspBI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
GlaI GCGC 7 cut(s) 116, 243, 509, 1235, 1868, 1870, 2327
GsaI CCCAGC 3 cut(s) 553, 1876, 2582
GsuI CTGGAG 4 cut(s) 676, 740, 1620, 2979
HaeII RGCGCY 2 cut(s) 245, 2329
HaeIII GGCC 5 cut(s) 554, 1516, 2106, 2186, 3053
HapII CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
HgaI GACGC 2 cut(s) 73, 2680
HhaI GCGC 7 cut(s) 117, 244, 510, 1236, 1869, 1871, 2328
Hin1I GRCGYC 1 cut(s) 2691
Hin6I GCGC 7 cut(s) 115, 242, 508, 1234, 1867, 1869, 2326
HinP1I GCGC 7 cut(s) 115, 242, 508, 1234, 1867, 1869, 2326
HincII GTYRAC 2 cut(s) 2907, 2947
HindII GTYRAC 2 cut(s) 2907, 2947
HindIII AAGCTT 3 cut(s) 1559, 2567, 2669
HinfI GANTC 4 cut(s) 422, 1309, 1654, 2494
HpaI GTTAAC 2 cut(s) 2907, 2947
HpaII CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
HphI GGTGA 7 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2367
Hpy166II GTNNAC 5 cut(s) 290, 726, 2816, 2907, 2947
Hpy188I TCNGA 7 cut(s) 51, 315, 1659, 2304, 2309, 2667, 2809
Hpy8I GTNNAC 5 cut(s) 290, 726, 2816, 2907, 2947
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 6 cut(s) 626, 940, 1102, 2067, 2096, 2914
HpyCH4III ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
HpyCH4IV ACGT 4 cut(s) 583, 841, 1912, 2903
HpyF3I CTNAG 8 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637, 2653
HpySE526I ACGT 4 cut(s) 583, 841, 1912, 2903
Hsp92I GRCGYC 1 cut(s) 2691
HspAI GCGC 7 cut(s) 115, 242, 508, 1234, 1867, 1869, 2326
KpnI GGTACC 1 cut(s) 1929
Ksp22I TGATCA 1 cut(s) 1809
KspAI GTTAAC 2 cut(s) 2907, 2947
LmnI GCTCC 7 cut(s) 84, 94, 759, 1285, 2428, 2965, 2974
Lsp1109I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
LweI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
MaeI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
MaeII ACGT 4 cut(s) 583, 841, 1912, 2903
MaeIII GTNAC 9 cut(s) 212, 277, 1051, 1245, 1378, 1496, 1900, 2075, 2546
MbiI CCGCTC 1 cut(s) 1795
MfeI CAATTG 2 cut(s) 1316, 2097
MflI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
MhlI GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
MlyI GAGTC 1 cut(s) 431
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 2 cut(s) 2247, 2924
MslI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
MspA1I CMGCKG 3 cut(s) 2092, 2582, 3020
MspCI CTTAAG 2 cut(s) 1250, 1735
MspI CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
MunI CAATTG 2 cut(s) 1316, 2097
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
MvnI CGCG 4 cut(s) 84, 115, 1236, 1869
NciI CCSGG 1 cut(s) 2112
NdeI CATATG 1 cut(s) 1606
NlaIV GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
NmuCI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 3 cut(s) 19, 1045, 1162
PaeI GCATGC 2 cut(s) 19, 1045
PaqCI CACCTGC 1 cut(s) 1942
PauI GCGCGC 1 cut(s) 1867
PcsI WCGNNNNNNNCGW 1 cut(s) 1233
PctI GAATGC 1 cut(s) 1125
PdmI GAANNNNTTC 2 cut(s) 2247, 2924
PfeI GAWTC 3 cut(s) 1309, 1654, 2494
Pfl23II CGTACG 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 376
PfoI TCCNGGA 2 cut(s) 2755, 2971
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1911
Psp124BI GAGCTC 2 cut(s) 99, 2433
Psp6I CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
PspEI GGTNACC 2 cut(s) 1496, 2075
PspFI CCCAGC 3 cut(s) 549, 1872, 2578
PspGI CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
PspLI CGTACG 1 cut(s) 110
PspN4I GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
PspPI GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
PstI CTGCAG 5 cut(s) 783, 1206, 1414, 2143, 3025
PstNI CAGNNNCTG 2 cut(s) 2147, 2618
PsuI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
PteI GCGCGC 1 cut(s) 1867
PvuI CGATCG 1 cut(s) 559
PvuII CAGCTG 2 cut(s) 2582, 3020
RseI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SacI GAGCTC 2 cut(s) 99, 2433
Sau96I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
SfaNI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
SfcI CTRYAG 9 cut(s) 466, 779, 1202, 1410, 1649, 2139, 2226, 2955, 3021
SinI GGWCC 3 cut(s) 2087, 2657, 2975
SmiMI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SmlI CTYRAG 2 cut(s) 1250, 1735
SmoI CTYRAG 2 cut(s) 1250, 1735
SpeI ACTAGT 1 cut(s) 2437
SphI GCATGC 2 cut(s) 19, 1045
SsiI CCGC 9 cut(s) 82, 419, 473, 1039, 1795, 1842, 1917, 2090, 2500
SspI AATATT 5 cut(s) 400, 1009, 1671, 2236, 2299
SspMI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
SstI GAGCTC 2 cut(s) 99, 2433
StyI CCWWGG 2 cut(s) 2071, 2459
TaaI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
TaiI ACGT 4 cut(s) 586, 844, 1915, 2906
TaqI TCGA 4 cut(s) 87, 1012, 1695, 2526
TaqII GACCGA 1 cut(s) 1919
TatI WGTACW 3 cut(s) 723, 1214, 2813
TauI GCSGC 1 cut(s) 1798
TfiI GAWTC 3 cut(s) 1309, 1654, 2494
TscAI CASTG 8 cut(s) 799, 1477, 2178, 2480, 2619, 2645, 2706, 2985
TseFI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TseI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
Tsp45I GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TspDTI ATGAA 8 cut(s) 25, 312, 375, 594, 1404, 2240, 2301, 2917
TspGWI ACGGA 5 cut(s) 352, 872, 1070, 1352, 3047
TspRI CASTG 8 cut(s) 799, 1477, 2178, 2480, 2619, 2645, 2706, 2985
Van91I CCANNNNNTGG 1 cut(s) 376
Vha464I CTTAAG 2 cut(s) 1250, 1735
VpaK11BI GGWCC 3 cut(s) 2087, 2657, 2975
XagI CCTNNNNNAGG 1 cut(s) 1738
XapI RAATTY 6 cut(s) 413, 983, 1274, 1490, 1784, 2594
XceI RCATGY 3 cut(s) 19, 1045, 1162
XmaJI CCTAGG 1 cut(s) 2459
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 2247, 2924
XspI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.