Rorug05G0539500

Protein TRANSPORT INHIBITOR RESPONSE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005204
Physical Location & Seq
Forward (+)
72791743 .. 72795084
3342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0539500.1

Sequence Viewer

Length: 2556 bp
ATGATGGGTTTGTTCTGTTTTTTGGTTCTGCTTCTCTTTCACTCTCTAAATTTCACTTCAGCTGCTTCTAATGAGGATGTGGTGAAGAAGCTTTTGTTGCAGTTCAAGCAATCCTCTGTTCAATCTGACCCTCATGGTTTCTTATCTGACTGGAAATCTGATTCTTCAACTGCCCTTTGTTCATGGAAAGGCCTGACTTGCTCTGAAGGCCACGTCATCACCCTCGACCTCTCCTATTCTGGTCTCATTGGAAGTCTTCACCTTCCAACTCTCACAGCCTTGCCAAGTCTTCAAAATCTGTACTTGCAAGGCAATTCATTCTCTGCTTCTGATCTTTCAGTTTCCAACATTACCACATGTAGCCTTGTAACAGTTGACTTGTCCTCCAACAACATCACAAGCCCTCTTCCGGTTCGGTCTTTTCTCGACGGTTGTGAGCATCTGGCTTTGGTCAACCTCTCTGGCAATTCAATCCCAGGAGGGAGTTTCAGCTTTGGTGCTTCCTTGCTTCAGCTTGACATCTCTAGAAACCTGATTTCGGATTCATCCTTGTTGACTTGCCAGAATCTGAATCTGCTCAATGTTTCCGGTAACAAGCTGACCGGAAAACTGAGTGGTTCTATCTTGTCTTGCAAGAATCTGACAACACTTGACCTTTCATACAATGCTTTATCTGGGGAGATACCCAACACTTTCCTGGAAAGCGGCTCTGCATCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTCACTGGAAAGTTTGCAAGCCTTGATTTCGTGCAGTGTAGCAGCCTCACGTTGCTGAAGCTATCACACAATAATCTCTATGGGGATGAGTTTCCTTCAAGCCTTGCTCACTGTCAAGCTTTGGAGACACTGAACCTGACTAGTAATATGTTGCAGGACAAAATTCCGGGTGCTTTGTTGGGAAATCTCAAGAAGTTGAGGCAACTCTTTCTGGGTCGCAATCAGTTTTCGGGTGAAATTCCAGCCGAATTAGGAAAGGCTTGTGGGACACTTGAGGAGCTTGATATTTCTGATAACATTCTTACTGGTGAATTGCCTTCAAGTTTTGTGTCGTGCTCTTCTTTGGTTAGTCTCAATCTTGGTCGCAATCAGCTCTCAGGAAATTTCCTCAGTACTGTTGTGAGTAAGCTTCCGAGTTTGACATATCTCTATGTACCCTTCAACAACATCACTGGTCCTGTGCCACCATCTATTACCAATGGTACTCGGCTTCAAGTGCTCGATCTCAGCGCTAATCTCTTCACAGGGAATGTTCCTTCAGTGTTTTGCTTCTCCAATGCTCCCTCAGCTTTGGAAAAGATACTTCTAGCCAACAATTTTCTCTCCGGGACTGTGCCATCGGAACTTGGAAACTGCAAGAGCCTGAAGGCTATTGATCTGAGTTTCAACAGTTTGAGTGGTGCAATTCCTTTAGAAGTTTGGACCTTGCCAAATCTTTCCGACTTGGTTATGTGGGCAAACAATCTCACTGGTGAAATCCCGGAAGGCATTTGCATCAATGGAGGAAACCTAGAAACCCTGATTCTCAATAATAATCTCATTACTGGAGCCATTCCACAGTCCATTGGCAGATGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGGCTTACAGGAGCAATCCCTTCAGGTATTGGAAATCTTCTTAAGCTTGCTATACTCCAGTTGGGTAATAATTCATTGTCTGGTCAGATTCCAGCTGAGCTTGGCAAGTGTCAAAGCCTCATTTGGCTTGACCTGAACAGCAATGACCTATATGGTTCTATCCCATCAGAGCTTGCTAACCAGGCCGGCCTAGTCAATCCTGGAATAGTTTCTGGGAAGCAGTTTGCATTTGTTAGAAATGAGGGTGGTACAGCTTGCAGGGGTGCAGGAGGACTAGTTGAGTTTGTGGGAGTCCGACCTCAGAGACTCGAAAGTTTACCTATGATTCATTCTTGCCCATCAACTAGAATTTACACAGGAATGACAGTTTACACATTCACCAGCAATGGAAGCATGATCTTCCTTGACATTTCCTACAATTCCTTGTCCGGGACTATTCCTGAAAACTTAGGTACACTGTCATATTTGCAAGTCTTCAATCTAGGACACAATATGTTAAGTGGAAATATTCCAGAAAGCTTTGGCGGATTAAAAGCAGTTGGAGTCCTGGATCTCTCTCACAATAATCTTGAAGGTTATGTCCCAGGATCTTTGGGGACTCTCTCTTTCCTGAGTGACCTTGATGTGTCTAACAACAACCTCACCGGTTTCATCCCTTCCGGAGGTCAGCTCACCACTTTCCCAGCATCAAGATATGAGAACAACTCCGGCCTTTGTGGCATGCCCTTGCCACCCTGTGGATCTCAAAGACATCCAACCCAATCTAAAGTTGTTCCTGAAAAAGAGAAGACTACAGTTACAGGAATGGTCGTTGGTTATGCTTCTTTCACTTTTTTCTTTGTGGTGACTGTGTATCTCTCTTTGTTAACACCCTATCTTGACCTCCCATTCCGGATTCATCTCAAGGGTTTCTCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000822 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006464 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007584 GO:0008144 GO:0008150 GO:0008152 GO:0009267 GO:0009555 GO:0009605 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0009991 GO:0010011 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010152 GO:0010311 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0016567 GO:0016740 GO:0019005 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0036094 GO:0036211 GO:0038023 GO:0038198 GO:0042221 GO:0042562 GO:0042594 GO:0043167 GO:0043168 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045013 GO:0045014 GO:0045892 GO:0045934 GO:0045990 GO:0046015 GO:0048229 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060089 GO:0060255 GO:0061458 GO:0061984 GO:0061985 GO:0061986 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071496 GO:0071704 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1905393 GO:1990234 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

851

Amino Acids

90.68

Weight (kDa)

5.44

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 68 6.8e-09 Leucine rich repeat N-terminal domain
LRR_4 PF12799 212 - 257 6.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 282 - 392 3e-06 Leucine-rich repeat region
LRR_8 PF13855 339 - 399 1.4e-07 Leucine rich repeat
Island PF20141 608 - 672 5.7e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 680 - 735 6e-08 Leucine rich repeat
LRR_8 PF13855 700 - 759 9.6e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2372
AccIII TCCGGA 2 cut(s) 2294, 2526
AciI CCGC 2 cut(s) 705, 2160
AclWI GGATC 4 cut(s) 738, 2193, 2230, 2383
AcsI RAATTY 5 cut(s) 49, 909, 984, 1129, 1983
AcuI CTGAAG 7 cut(s) 42, 225, 494, 824, 1269, 1412, 1641
AdeI CACNNNGTG 1 cut(s) 2372
AfaI GTAC 6 cut(s) 302, 1141, 1182, 1231, 1885, 2089
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 5 cut(s) 409, 538, 2064, 2297, 2372
AflII CTTAAG 1 cut(s) 1676
AflIII ACRYGT 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 2279
AhlI ACTAGT 2 cut(s) 887, 1909
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 6 cut(s) 475, 696, 1815, 1834, 2181, 2218
Alw21I GWGCWC 2 cut(s) 1085, 1248
Alw26I GTCTC 4 cut(s) 248, 866, 1103, 1933
AlwI GGATC 4 cut(s) 738, 2193, 2230, 2383
AlwNI CAGNNNCTG 1 cut(s) 568
Aor13HI TCCGGA 2 cut(s) 2294, 2526
Aor51HI AGCGCT 1 cut(s) 1258
AoxI GGCC 5 cut(s) 190, 208, 1818, 1822, 2344
ApeKI GCWGC 2 cut(s) 62, 789
ApoI RAATTY 5 cut(s) 49, 909, 984, 1129, 1983
AsiGI ACCGGT 1 cut(s) 2279
Asp700I GAANNNNTTC 1 cut(s) 1843
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 2 cut(s) 1202, 1449
AsuC2I CCSGG 4 cut(s) 915, 1354, 1508, 2065
AvaII GGWCC 2 cut(s) 1202, 1449
BbsI GAAGAC 4 cut(s) 248, 281, 2101, 2429
Bbv12I GWGCWC 2 cut(s) 1085, 1248
BbvCI CCTCAGC 1 cut(s) 1312
BbvI GCAGC 2 cut(s) 49, 801
BccI CCATC 4 cut(s) 1222, 1372, 1807, 1981
BciT130I CCWGG 6 cut(s) 477, 698, 1817, 1836, 2183, 2220
BcnI CCSGG 4 cut(s) 915, 1354, 1508, 2065
BcoDI GTCTC 4 cut(s) 248, 866, 1103, 1933
BcuI ACTAGT 2 cut(s) 887, 1909
BfaI CTAG 9 cut(s) 525, 888, 1334, 1538, 1629, 1826, 1910, 1980, 2117
BfmI CTRYAG 1 cut(s) 2427
BfoI RGCGCY 1 cut(s) 1260
BfrI CTTAAG 1 cut(s) 1676
BglI GCCNNNNNGGC 1 cut(s) 2352
BisI GCNGC 3 cut(s) 63, 706, 790
BlpI GCTNAGC 1 cut(s) 1731
BlsI GCNGC 3 cut(s) 64, 707, 791
BmcAI AGTACT 1 cut(s) 1141
Bme18I GGWCC 2 cut(s) 1202, 1449
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 1202, 1449
BmiI GGNNCC 1 cut(s) 1576
BmsI GCATC 5 cut(s) 448, 722, 1530, 1589, 2330
BpiI GAAGAC 4 cut(s) 248, 281, 2101, 2429
BplI GAGNNNNNCTC 4 cut(s) 2289, 2321, 2324, 2356
BpmI CTGGAG 2 cut(s) 1593, 1676
Bpu10I CCTNAGC 1 cut(s) 1312
Bpu1102I GCTNAGC 1 cut(s) 1731
BpuEI CTTGAG 5 cut(s) 704, 721, 920, 1040, 2522
BpuMI CCSGG 4 cut(s) 915, 1354, 1508, 2065
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 475, 2218
BsaWI WCCGGW 6 cut(s) 409, 587, 602, 2279, 2294, 2526
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 1896, 1926
Bsc4I CCNNNNNNNGG 5 cut(s) 409, 538, 2064, 2297, 2372
Bse118I RCCGGY 3 cut(s) 1635, 1820, 2279
Bse1I ACTGG 7 cut(s) 155, 757, 1057, 1204, 1501, 1576, 1693
Bse3DI GCAATG 2 cut(s) 1783, 2026
BseAI TCCGGA 2 cut(s) 2294, 2526
BseBI CCWGG 6 cut(s) 477, 698, 1817, 1836, 2183, 2220
BseDI CCNNGG 2 cut(s) 475, 2218
BseGI GGATG 5 cut(s) 82, 545, 838, 2286, 2386
BseLI CCNNNNNNNGG 5 cut(s) 409, 538, 2064, 2297, 2372
BseMI GCAATG 2 cut(s) 1783, 2026
BseMII CTCAG 9 cut(s) 602, 1137, 1150, 1267, 1326, 1397, 1722, 1949, 2237
BseNI ACTGG 7 cut(s) 155, 757, 1057, 1204, 1501, 1576, 1693
BseRI GAGGAG 1 cut(s) 1037
BseXI GCAGC 2 cut(s) 49, 801
BseYI CCCAGC 1 cut(s) 2317
BsgI GTGCAG 2 cut(s) 800, 1920
BshFI GGCC 5 cut(s) 192, 210, 1820, 1824, 2346
BshTI ACCGGT 1 cut(s) 2279
BsiHKAI GWGCWC 2 cut(s) 1085, 1248
BslFI GGGAC 5 cut(s) 1027, 1369, 2080, 2201, 2245
BslI CCNNNNNNNGG 5 cut(s) 409, 538, 2064, 2297, 2372
BsmAI GTCTC 4 cut(s) 248, 866, 1103, 1933
BsmFI GGGAC 5 cut(s) 1027, 1369, 2080, 2201, 2245
BsnI GGCC 5 cut(s) 192, 210, 1820, 1824, 2346
Bso31I GGTCTC 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 1085, 1248
Bsp13I TCCGGA 2 cut(s) 2294, 2526
Bsp143I GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
Bsp1720I GCTNAGC 1 cut(s) 1731
BspACI CCGC 2 cut(s) 705, 2160
BspANI GGCC 5 cut(s) 192, 210, 1820, 1824, 2346
BspCNI CTCAG 9 cut(s) 603, 1136, 1149, 1266, 1325, 1398, 1723, 1948, 2238
BspEI TCCGGA 2 cut(s) 2294, 2526
BspLI GGNNCC 1 cut(s) 1576
BspPI GGATC 4 cut(s) 738, 2193, 2230, 2383
BspQI GCTCTTC 1 cut(s) 1090
BspTI CTTAAG 1 cut(s) 1676
BspTNI GGTCTC 1 cut(s) 248
BsrDI GCAATG 2 cut(s) 1783, 2026
BsrFI RCCGGY 3 cut(s) 1635, 1820, 2279
BsrI ACTGG 7 cut(s) 155, 757, 1057, 1204, 1501, 1576, 1693
BssAI RCCGGY 3 cut(s) 1635, 1820, 2279
BssECI CCNNGG 2 cut(s) 475, 2218
BssMI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
Bst2UI CCWGG 6 cut(s) 477, 698, 1817, 1836, 2183, 2220
Bst6I CTCTTC 3 cut(s) 411, 1090, 1271
BstAFI CTTAAG 1 cut(s) 1676
BstC8I GCNNGC 6 cut(s) 766, 1683, 1809, 1822, 1891, 2357
BstENI CCTNNNNNAGG 1 cut(s) 2295
BstF5I GGATG 5 cut(s) 82, 545, 838, 2286, 2386
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 8 cut(s) 334, 733, 1252, 1405, 2034, 2188, 2225, 2378
BstMAI GTCTC 4 cut(s) 248, 866, 1103, 1933
BstMBI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
BstMWI GCNNNNNNNGC 9 cut(s) 97, 106, 198, 207, 1243, 1313, 1817, 2025, 2352
BstNI CCWGG 6 cut(s) 477, 698, 1817, 1836, 2183, 2220
BstNSI RCATGY 2 cut(s) 360, 2359
BstSFI CTRYAG 1 cut(s) 2427
BstV1I GCAGC 2 cut(s) 49, 801
BstV2I GAAGAC 4 cut(s) 248, 281, 2101, 2429
BstX2I RGATCY 4 cut(s) 730, 2185, 2222, 2375
BstYI RGATCY 4 cut(s) 730, 2185, 2222, 2375
BsuRI GGCC 5 cut(s) 192, 210, 1820, 1824, 2346
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 5 cut(s) 82, 545, 838, 2286, 2386
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 8 cut(s) 750, 788, 856, 875, 1197, 1293, 1494, 2090
Cac8I GCNNGC 6 cut(s) 766, 1683, 1809, 1822, 1891, 2357
CaiI CAGNNNCTG 1 cut(s) 568
CfoI GCGC 1 cut(s) 1259
Cfr10I RCCGGY 3 cut(s) 1635, 1820, 2279
Cfr13I GGNCC 2 cut(s) 1202, 1449
Csp6I GTAC 6 cut(s) 301, 1140, 1181, 1230, 1884, 2088
CspAI ACCGGT 1 cut(s) 2279
CviAII CATG 5 cut(s) 134, 183, 357, 2029, 2356
CviQI GTAC 6 cut(s) 301, 1140, 1181, 1230, 1884, 2088
DpnI GATC 8 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224, 2377
DpnII GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
DraIII CACNNNGTG 1 cut(s) 2372
Eam1104I CTCTTC 3 cut(s) 411, 1090, 1271
EarI CTCTTC 3 cut(s) 411, 1090, 1271
EciI GGCGGA 1 cut(s) 2175
Eco147I AGGCCT 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 1202, 1449
Eco47III AGCGCT 1 cut(s) 1258
Eco57I CTGAAG 7 cut(s) 42, 225, 494, 824, 1269, 1412, 1641
EcoNI CCTNNNNNAGG 1 cut(s) 2295
EcoRII CCWGG 6 cut(s) 475, 696, 1815, 1834, 2181, 2218
FaeI CATG 5 cut(s) 137, 186, 360, 2032, 2359
FaqI GGGAC 5 cut(s) 1027, 1369, 2080, 2201, 2245
FatI CATG 5 cut(s) 133, 182, 356, 2028, 2355
Fnu4HI GCNGC 3 cut(s) 63, 706, 790
FokI GGATG 5 cut(s) 89, 532, 845, 2273, 2373
FseI GGCCGGCC 1 cut(s) 1824
Fsp4HI GCNGC 3 cut(s) 63, 706, 790
FspBI CTAG 9 cut(s) 525, 888, 1334, 1538, 1629, 1826, 1910, 1980, 2117
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 3 cut(s) 63, 706, 790
GsaI CCCAGC 1 cut(s) 2321
GsuI CTGGAG 2 cut(s) 1593, 1676
HaeII RGCGCY 1 cut(s) 1260
HaeIII GGCC 5 cut(s) 192, 210, 1820, 1824, 2346
HhaI GCGC 1 cut(s) 1259
Hin1II CATG 5 cut(s) 137, 186, 360, 2032, 2359
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 4 cut(s) 376, 454, 555, 2502
HindII GTYRAC 4 cut(s) 376, 454, 555, 2502
HindIII AAGCTT 5 cut(s) 89, 864, 1154, 1679, 2152
HpaI GTTAAC 1 cut(s) 2502
Hpy166II GTNNAC 7 cut(s) 376, 454, 555, 1952, 2005, 2090, 2502
Hpy8I GTNNAC 7 cut(s) 376, 454, 555, 1952, 2005, 2090, 2502
Hpy99I CGWCG 1 cut(s) 431
HpyCH4IV ACGT 2 cut(s) 213, 797
HpyF10VI GCNNNNNNNGC 9 cut(s) 97, 106, 198, 207, 1243, 1313, 1817, 2025, 2352
HpySE526I ACGT 2 cut(s) 213, 797
Hsp92II CATG 5 cut(s) 137, 186, 360, 2032, 2359
HspAI GCGC 1 cut(s) 1257
Kpn2I TCCGGA 2 cut(s) 2294, 2526
KroI GCCGGC 1 cut(s) 1820
KroNI GCCGGC 1 cut(s) 1822
KspAI GTTAAC 1 cut(s) 2502
Kzo9I GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
LguI GCTCTTC 1 cut(s) 1090
LmnI GCTCC 4 cut(s) 1024, 1312, 1574, 1646
Lsp1109I GCAGC 2 cut(s) 49, 801
LweI GCATC 5 cut(s) 448, 722, 1530, 1589, 2330
MaeI CTAG 9 cut(s) 525, 888, 1334, 1538, 1629, 1826, 1910, 1980, 2117
MaeII ACGT 2 cut(s) 213, 797
MaeIII GTNAC 6 cut(s) 367, 590, 1620, 2249, 2431, 2479
MalI GATC 8 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224, 2377
MboI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
MflI RGATCY 4 cut(s) 730, 2185, 2222, 2375
MhlI GDGCHC 2 cut(s) 1085, 1248
MlyI GAGTC 4 cut(s) 1935, 1935, 2187, 2227
MmeI TCCRAC 7 cut(s) 290, 369, 411, 1491, 1954, 2155, 2414
MroI TCCGGA 2 cut(s) 2294, 2526
MroNI GCCGGC 1 cut(s) 1820
MroXI GAANNNNTTC 1 cut(s) 1843
MseI TTAA 4 cut(s) 1677, 2132, 2165, 2501
MslI CAYNNNNRTG 2 cut(s) 1607, 1994
MspA1I CMGCKG 2 cut(s) 62, 1730
MspCI CTTAAG 1 cut(s) 1676
MvaI CCWGG 6 cut(s) 477, 698, 1817, 1836, 2183, 2220
MwoI GCNNNNNNNGC 9 cut(s) 97, 106, 198, 207, 1243, 1313, 1817, 2025, 2352
NaeI GCCGGC 1 cut(s) 1822
NciI CCSGG 4 cut(s) 915, 1354, 1508, 2065
NdeII GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
NgoMIV GCCGGC 1 cut(s) 1820
NlaIII CATG 5 cut(s) 137, 186, 360, 2032, 2359
NlaIV GGNNCC 1 cut(s) 1576
NmeAIII GCCGAG 1 cut(s) 1213
NmuCI GTSAC 3 cut(s) 1620, 2249, 2479
NspI RCATGY 2 cut(s) 360, 2359
PaeI GCATGC 1 cut(s) 2359
PceI AGGCCT 1 cut(s) 192
PciI ACATGT 1 cut(s) 356
PciSI GCTCTTC 1 cut(s) 1090
PdiI GCCGGC 1 cut(s) 1822
PdmI GAANNNNTTC 1 cut(s) 1843
PfeI GAWTC 9 cut(s) 161, 542, 565, 571, 637, 1549, 1723, 1960, 2530
PflMI CCANNNNNTGG 1 cut(s) 2372
PfoI TCCNGGA 6 cut(s) 696, 1352, 1506, 1834, 2063, 2181
PinAI ACCGGT 1 cut(s) 2279
PkrI GCNGC 3 cut(s) 64, 707, 791
PleI GAGTC 4 cut(s) 1934, 1935, 2186, 2227
PpsI GAGTC 4 cut(s) 1934, 1935, 2186, 2227
PscI ACATGT 1 cut(s) 356
Psp6I CCWGG 6 cut(s) 475, 696, 1815, 1834, 2181, 2218
PspFI CCCAGC 1 cut(s) 2317
PspGI CCWGG 6 cut(s) 475, 696, 1815, 1834, 2181, 2218
PspN4I GGNNCC 1 cut(s) 1576
PspPI GGNCC 2 cut(s) 1202, 1449
PstNI CAGNNNCTG 1 cut(s) 568
PsuI RGATCY 4 cut(s) 730, 2185, 2222, 2375
PvuII CAGCTG 2 cut(s) 62, 1730
RigI GGCCGGCC 1 cut(s) 1824
RsaI GTAC 6 cut(s) 302, 1141, 1182, 1231, 1885, 2089
RsaNI GTAC 6 cut(s) 301, 1140, 1181, 1230, 1884, 2088
RseI CAYNNNNRTG 2 cut(s) 1607, 1994
SapI GCTCTTC 1 cut(s) 1090
SaqAI TTAA 4 cut(s) 1677, 2132, 2165, 2501
SatI GCNGC 3 cut(s) 63, 706, 790
Sau3AI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2375
Sau96I GGNCC 2 cut(s) 1202, 1449
ScaI AGTACT 1 cut(s) 1141
SchI GAGTC 4 cut(s) 1935, 1935, 2187, 2227
SduI GDGCHC 2 cut(s) 1085, 1248
SfaNI GCATC 5 cut(s) 448, 722, 1530, 1589, 2330
SfcI CTRYAG 1 cut(s) 2427
SinI GGWCC 2 cut(s) 1202, 1449
SmiMI CAYNNNNRTG 2 cut(s) 1607, 1994
SmlI CTYRAG 6 cut(s) 719, 736, 935, 1019, 1676, 2537
SmoI CTYRAG 6 cut(s) 719, 736, 935, 1019, 1676, 2537
SpeI ACTAGT 2 cut(s) 887, 1909
SphI GCATGC 1 cut(s) 2359
SseBI AGGCCT 1 cut(s) 192
SsiI CCGC 2 cut(s) 705, 2160
SspI AATATT 1 cut(s) 2143
SspMI CTAG 9 cut(s) 525, 888, 1334, 1538, 1629, 1826, 1910, 1980, 2117
StuI AGGCCT 1 cut(s) 192
TaiI ACGT 2 cut(s) 216, 800
TaqI TCGA 4 cut(s) 225, 426, 1248, 1944
TaqII GACCGA 1 cut(s) 405
TatI WGTACW 2 cut(s) 300, 1139
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 9 cut(s) 161, 542, 565, 571, 637, 1549, 1723, 1960, 2530
Tru1I TTAA 4 cut(s) 1677, 2132, 2165, 2501
Tru9I TTAA 4 cut(s) 1677, 2132, 2165, 2501
TscAI CASTG 8 cut(s) 757, 788, 863, 882, 1204, 1293, 1501, 2097
TseFI GTSAC 3 cut(s) 1620, 2249, 2479
TseI GCWGC 2 cut(s) 62, 789
Tsp45I GTSAC 3 cut(s) 1620, 2249, 2479
TspDTI ATGAA 8 cut(s) 171, 306, 534, 648, 1698, 1952, 2275, 2522
TspRI CASTG 8 cut(s) 757, 788, 863, 882, 1204, 1293, 1501, 2097
Van91I CCANNNNNTGG 1 cut(s) 2372
Vha464I CTTAAG 1 cut(s) 1676
VpaK11BI GGWCC 2 cut(s) 1202, 1449
XagI CCTNNNNNAGG 1 cut(s) 2295
XapI RAATTY 5 cut(s) 49, 909, 984, 1129, 1983
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 2 cut(s) 360, 2359
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmnI GAANNNNTTC 1 cut(s) 1843
XspI CTAG 9 cut(s) 525, 888, 1334, 1538, 1629, 1826, 1910, 1980, 2117
ZrmI AGTACT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.