Rh1DG094900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003955
Physical Location & Seq
Forward (+)
17999129 .. 18001477
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG094900.1

Sequence Viewer

Length: 939 bp
ATGAAACCCTCCTCCTCCTCCTCTTCTTCTTCTTCACCATGCGGATCAAACACGCTCTTAACGATTCTGTTCCAGTCGAGATCCGAAGCCTGCAGCAACGATTCTATTCTCACAGACTTGGCCGCCAGAGAATTCAACGCCTTCCTCTGGATCTCTCTCATCGCCGTCACCGCTCTGTTGCTCCGGAAGCTCTTCGACCTGCTTCGCTTATGGGCCAAAGCCAAGCCCATCCCTGGGCCTCCTTGCCCCTCTTTCTACGGCCACTCCAACCTCCTTTCCCGCCAAAATATTACTGAGGTGTTGGGTGATTTGCACAAGAAATATGGCTCTGTTGTGAAGTTGTGGTTGGGACCGACTCGGCTTTTAGTGTCAATAAAGGATCCAATTCTTATCAAAGAGATGCTACTAAAGGCTGCAGATAAGTTACCTATGACTGGGAGGGCATTCCATTTGGCCTTTGGAAGGTCAAGTCTCTTTGCTTCTTCTTTCGACGAGGTGCAAAAGAGAAGGGAAACATTATTAACAGAATTAAATGGAAAAGTTCGTGAGAACAAAAATGTGTTCCCCACCAAGGCTGTGGACTGTGTCTTGGAGAGGATAAACAATGTAATGGGTAAAGATAGTGTTGACAGTAGAGTATTTTCTCAACACATGGCTTTTACCATGTTAGGAGCCACCCTCTTTGGAGATGCATTTTTAGCTTGGTCTAAGGCAGCTGTTTACGAGGAGCTCCTTATGATGATTGCTAAGGATGCTGGTTTTTGGGCTTCTTATTGTGTTACTCCCTTTTGGAAGCCTGGATTTTGGAAATATCAGTCTGTATGCACAAAGTTGAAATGCTTAACGCAAGATATTGTTCAACAATGCTGCAACCTATACTCTTGGGAAACTGGAAACTTAGGAAAGGAGGTTTGCATCTGGTGGACCATCTTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

312

Amino Acids

35.0

Weight (kDa)

9.11

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 79 - 289 9e-13 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 207
AccBSI CCGCTC 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 4 cut(s) 42, 123, 171, 280
AclWI GGATC 5 cut(s) 52, 75, 158, 374, 387
AcoI YGGCCR 2 cut(s) 120, 259
AcsI RAATTY 1 cut(s) 131
AfiI CCNNNNNNNGG 6 cut(s) 147, 233, 234, 434, 462, 571
AgsI TTSAA 3 cut(s) 136, 835, 860
AjnI CCWGG 2 cut(s) 232, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 4 cut(s) 190, 701, 716, 730
AluI AGCT 4 cut(s) 190, 701, 716, 730
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 5 cut(s) 52, 75, 158, 374, 387
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 5 cut(s) 120, 213, 236, 259, 453
ApeKI GCWGC 4 cut(s) 93, 413, 713, 867
ApoI RAATTY 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 191
AspS9I GGNCC 4 cut(s) 213, 236, 350, 924
AsuHPI GGTGA 3 cut(s) 27, 160, 317
AvaII GGWCC 2 cut(s) 350, 924
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 732
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 4 cut(s) 105, 400, 725, 854
BccI CCATC 2 cut(s) 236, 935
BceAI ACGGC 2 cut(s) 149, 274
BciT130I CCWGG 2 cut(s) 234, 798
BcoDI GTCTC 1 cut(s) 476
BfmI CTRYAG 2 cut(s) 91, 414
BfuAI ACCTGC 1 cut(s) 207
BisI GCNGC 5 cut(s) 94, 123, 414, 714, 868
BlsI GCNGC 5 cut(s) 95, 124, 415, 715, 869
Bme1390I CCNGG 2 cut(s) 234, 798
Bme18I GGWCC 2 cut(s) 350, 924
BmgT120I GGNCC 4 cut(s) 213, 236, 350, 924
BmiI GGNNCC 3 cut(s) 351, 381, 673
BmrFI CCNGG 2 cut(s) 234, 798
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 390, 679, 742, 924
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 2 cut(s) 663, 695
Bpu10I CCTNAGC 1 cut(s) 747
BsaJI CCNNGG 3 cut(s) 232, 233, 570
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 6 cut(s) 147, 233, 234, 434, 462, 571
Bse1I ACTGG 3 cut(s) 73, 439, 895
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 2 cut(s) 234, 798
BseDI CCNNGG 3 cut(s) 232, 233, 570
BseGI GGATG 2 cut(s) 228, 757
BseLI CCNNNNNNNGG 6 cut(s) 147, 233, 234, 434, 462, 571
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 3 cut(s) 73, 439, 895
BseRI GAGGAG 4 cut(s) 4, 7, 10, 740
BseXI GCAGC 4 cut(s) 105, 400, 725, 854
BshFI GGCC 5 cut(s) 122, 215, 238, 261, 455
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 6 cut(s) 147, 233, 234, 434, 462, 571
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 5 cut(s) 122, 215, 238, 261, 455
Bsp1286I GDGCHC 1 cut(s) 732
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 4 cut(s) 44, 80, 150, 379
BspACI CCGC 4 cut(s) 42, 123, 171, 280
BspANI GGCC 5 cut(s) 122, 215, 238, 261, 455
BspCNI CTCAG 1 cut(s) 286
BspEI TCCGGA 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 351, 381, 673
BspMAI CTGCAG 2 cut(s) 95, 418
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 5 cut(s) 52, 75, 158, 374, 387
BspQI GCTCTTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 173
BsrI ACTGG 3 cut(s) 73, 439, 895
BssECI CCNNGG 3 cut(s) 232, 233, 570
BssMI GATC 4 cut(s) 44, 80, 150, 379
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 234, 798
Bst4CI ACNGT 2 cut(s) 584, 632
Bst6I CTCTTC 2 cut(s) 28, 197
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 4 cut(s) 294, 708, 747, 898
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 228, 757
BstKTI GATC 4 cut(s) 47, 83, 153, 382
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 4 cut(s) 44, 80, 150, 379
BstMWI GCNNNNNNNGC 4 cut(s) 170, 187, 698, 752
BstNI CCWGG 2 cut(s) 234, 798
BstSCI CCNGG 2 cut(s) 232, 796
BstSFI CTRYAG 2 cut(s) 91, 414
BstV1I GCAGC 4 cut(s) 105, 400, 725, 854
BstX2I RGATCY 3 cut(s) 80, 150, 379
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 3 cut(s) 80, 150, 379
BsuRI GGCC 5 cut(s) 122, 215, 238, 261, 455
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 2 cut(s) 228, 757
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 213, 236, 350, 924
CspCI CAANNNNNGTGG 2 cut(s) 664, 699
CviAII CATG 3 cut(s) 39, 652, 664
DdeI CTNAG 4 cut(s) 294, 708, 747, 898
DpnI GATC 4 cut(s) 46, 82, 152, 381
DpnII GATC 4 cut(s) 44, 80, 150, 379
EaeI YGGCCR 2 cut(s) 120, 259
Eam1104I CTCTTC 2 cut(s) 28, 197
EarI CTCTTC 2 cut(s) 28, 197
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 732
Eco47I GGWCC 2 cut(s) 350, 924
Eco53kI GAGCTC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 730
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 232, 796
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 3 cut(s) 42, 655, 667
FaiI YATR 9 cut(s) 40, 211, 324, 431, 653, 665, 737, 823, 877
FaqI GGGAC 1 cut(s) 363
FatI CATG 3 cut(s) 38, 651, 663
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 5 cut(s) 94, 123, 414, 714, 868
FokI GGATG 2 cut(s) 215, 764
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 5 cut(s) 94, 123, 414, 714, 868
GluI GCNGC 5 cut(s) 94, 123, 414, 714, 868
HaeIII GGCC 5 cut(s) 122, 215, 238, 261, 455
HapII CCGG 1 cut(s) 184
Hin1II CATG 3 cut(s) 42, 655, 667
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 3 cut(s) 64, 101, 355
HpaII CCGG 1 cut(s) 184
HphI GGTGA 3 cut(s) 27, 160, 317
Hpy166II GTNNAC 4 cut(s) 580, 628, 721, 924
Hpy188I TCNGA 2 cut(s) 85, 938
Hpy188III TCNNGA 4 cut(s) 78, 148, 184, 545
Hpy8I GTNNAC 4 cut(s) 580, 628, 721, 924
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 3 cut(s) 151, 456, 501
HpyCH4III ACNGT 2 cut(s) 584, 632
HpyCH4V TGCA 8 cut(s) 93, 313, 416, 499, 692, 825, 870, 915
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 187, 698, 752
HpyF3I CTNAG 4 cut(s) 294, 708, 747, 898
Hsp92II CATG 3 cut(s) 42, 655, 667
Kpn2I TCCGGA 1 cut(s) 183
Kzo9I GATC 4 cut(s) 44, 80, 150, 379
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 4 cut(s) 186, 671, 727, 735
Lsp1109I GCAGC 4 cut(s) 105, 400, 725, 854
LweI GCATC 4 cut(s) 390, 679, 742, 924
MaeIII GTNAC 3 cut(s) 166, 423, 778
MalI GATC 4 cut(s) 46, 82, 152, 381
MbiI CCGCTC 1 cut(s) 173
MboI GATC 4 cut(s) 44, 80, 150, 379
MboII GAAGA 6 cut(s) 15, 18, 21, 24, 184, 474
MflI RGATCY 3 cut(s) 80, 150, 379
MhlI GDGCHC 1 cut(s) 732
MluCI AATT 3 cut(s) 131, 384, 527
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 291
Mph1103I ATGCAT 1 cut(s) 694
MroI TCCGGA 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 4 cut(s) 59, 521, 530, 842
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 2 cut(s) 234, 798
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 2 cut(s) 234, 798
MwoI GCNNNNNNNGC 4 cut(s) 170, 187, 698, 752
NdeII GATC 4 cut(s) 44, 80, 150, 379
NlaIII CATG 3 cut(s) 42, 655, 667
NlaIV GGNNCC 3 cut(s) 351, 381, 673
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 694
PasI CCCWGGG 1 cut(s) 233
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 64, 101
PflFI GACNNNGTC 1 cut(s) 584
PkrI GCNGC 5 cut(s) 95, 124, 415, 715, 869
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 232, 796
PspGI CCWGG 2 cut(s) 232, 796
PspN4I GGNNCC 3 cut(s) 351, 381, 673
PspPI GGNCC 4 cut(s) 213, 236, 350, 924
PstI CTGCAG 2 cut(s) 95, 418
PsuI RGATCY 3 cut(s) 80, 150, 379
PsyI GACNNNGTC 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 732
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 4 cut(s) 59, 521, 530, 842
SatI GCNGC 5 cut(s) 94, 123, 414, 714, 868
Sau3AI GATC 4 cut(s) 44, 80, 150, 379
Sau96I GGNCC 4 cut(s) 213, 236, 350, 924
SchI GAGTC 1 cut(s) 349
ScrFI CCNGG 2 cut(s) 234, 798
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 4 cut(s) 390, 679, 742, 924
SfcI CTRYAG 2 cut(s) 91, 414
SinI GGWCC 2 cut(s) 350, 924
Sse9I AATT 3 cut(s) 131, 384, 527
SsiI CCGC 4 cut(s) 42, 123, 171, 280
SspI AATATT 1 cut(s) 289
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 232, 796
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 584, 632
TaqI TCGA 3 cut(s) 77, 195, 489
TaqII GACCGA 1 cut(s) 367
TasI AATT 3 cut(s) 131, 384, 527
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 2 cut(s) 64, 101
Tru1I TTAA 4 cut(s) 59, 521, 530, 842
Tru9I TTAA 4 cut(s) 59, 521, 530, 842
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 4 cut(s) 93, 413, 713, 867
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 350, 924
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 2 cut(s) 455, 574
XmnI GAANNNNTTC 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.