Rh2DG336600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006398
Physical Location & Seq
Forward (+)
43240166 .. 43254304
14139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG336600.1

Sequence Viewer

Length: 516 bp
ATGCTACTAGCAGACCACTCCGACGAGCTCACAGATCGGAGCCCCTCTCCGGCAGGATCCACTTTCCAGACCATCACCAACCTCCTCACCTTCATTCGACCAAATGATCGGTGGTCGATGATGCTCTCCAACACATCCCTAGATCGGGGCTCTGATCGGATCGTCACTCCAACTGGGCGGTTGGCCTCCATGTTCACACCACGAGGTGCTTTGGCCAGGGCCATCCCTCTGCAAGGTCCACCACCATTCTTCTCCGAGTACAAAGGCCATGGTTGTACCCCGATCGGATCGCCTGTATCTCCTCCTTCAGTCACTTCCTGCCTGGCCTGGATCAAATCCGATCTTCTGCCATCTTGCTCAAGAGAGCGGCGATACCCCCAGGTTCGGATTACACACTTGCTTTTCCTTGCGCACCAGATTGGAGACCTTGACATCATACCCCTGCTTGCAAGACGCGTCAGTCAGAGTACTTCGTGTGGAAACCCAAAGCTGGTGAGAAATCGTTCTGCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.78

Weight (kDa)

9.93

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 459
Acc16I TGCGCA 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 367
AccII CGCG 1 cut(s) 456
AciI CCGC 2 cut(s) 178, 367
AclWI GGATC 5 cut(s) 51, 64, 167, 295, 338
AcoI YGGCCR 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 206
AfaI GTAC 3 cut(s) 260, 277, 469
AfiI CCNNNNNNNGG 6 cut(s) 49, 144, 145, 233, 384, 490
AflIII ACRYGT 1 cut(s) 454
AjnI CCWGG 4 cut(s) 215, 321, 326, 378
AluBI AGCT 2 cut(s) 28, 490
AluI AGCT 2 cut(s) 28, 490
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 5 cut(s) 51, 64, 167, 295, 338
AoxI GGCC 5 cut(s) 183, 213, 219, 265, 324
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 2 cut(s) 219, 236
AsuHPI GGTGA 3 cut(s) 67, 79, 505
AvaII GGWCC 1 cut(s) 236
BalI TGGCCA 1 cut(s) 215
BamHI GGATCC 1 cut(s) 56
BanII GRGCYC 3 cut(s) 30, 44, 152
BauI CACGAG 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 30
BccI CCATC 3 cut(s) 80, 230, 358
BciT130I CCWGG 4 cut(s) 217, 323, 328, 380
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 2 cut(s) 8, 140
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 4 cut(s) 217, 323, 328, 380
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 219, 236
BmiI GGNNCC 2 cut(s) 41, 58
BmrFI CCNGG 4 cut(s) 217, 323, 328, 380
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 111
BmuI ACTGGG 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 31, 63
BpuEI CTTGAG 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 216, 268, 378
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 144, 145, 233, 384, 490
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 4 cut(s) 217, 323, 328, 380
BseDI CCNNGG 3 cut(s) 216, 268, 378
BseGI GGATG 2 cut(s) 134, 222
BseLI CCNNNNNNNGG 6 cut(s) 49, 144, 145, 233, 384, 490
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 2 cut(s) 74, 291
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 456
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 5 cut(s) 185, 215, 221, 267, 326
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 6 cut(s) 49, 144, 145, 233, 384, 490
BsmAI GTCTC 1 cut(s) 417
BsnI GGCC 5 cut(s) 185, 215, 221, 267, 326
Bso31I GGTCTC 1 cut(s) 417
Bsp1286I GDGCHC 3 cut(s) 30, 44, 152
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 2 cut(s) 178, 367
BspANI GGCC 5 cut(s) 185, 215, 221, 267, 326
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 41, 58
BspPI GGATC 5 cut(s) 51, 64, 167, 295, 338
BspTNI GGTCTC 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 216, 268, 378
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 268
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 4 cut(s) 217, 323, 328, 380
BstC8I GCNNGC 1 cut(s) 447
BstDSI CCRYGG 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 134, 222
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 412
BstMAI GTCTC 1 cut(s) 417
BstMCI CGRYCG 1 cut(s) 285
BstNI CCWGG 4 cut(s) 217, 323, 328, 380
BstSCI CCNGG 4 cut(s) 215, 321, 326, 378
BstUI CGCG 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 5 cut(s) 185, 215, 221, 267, 326
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 2 cut(s) 134, 222
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 219, 236
CseI GACGC 2 cut(s) 445, 462
Csp6I GTAC 3 cut(s) 259, 276, 468
CviAII CATG 2 cut(s) 190, 269
CviJI RGCY 9 cut(s) 28, 42, 150, 185, 215, 221, 267, 326, 490
CviKI_1 RGCY 9 cut(s) 28, 42, 150, 185, 215, 221, 267, 326, 490
CviQI GTAC 3 cut(s) 259, 276, 468
DraIII CACNNNGTG 1 cut(s) 206
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 268
Eco24I GRGCYC 3 cut(s) 30, 44, 152
Eco31I GGTCTC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoRII CCWGG 4 cut(s) 215, 321, 326, 378
EcoT14I CCWWGG 1 cut(s) 268
EcoT38I GRGCYC 3 cut(s) 30, 44, 152
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 2 cut(s) 193, 272
FaiI YATR 3 cut(s) 191, 270, 437
FatI CATG 2 cut(s) 189, 268
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 2 cut(s) 121, 209
FriOI GRGCYC 3 cut(s) 30, 44, 152
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 2 cut(s) 8, 140
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 1 cut(s) 411
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 5 cut(s) 185, 215, 221, 267, 326
HapII CCGG 1 cut(s) 50
HgaI GACGC 2 cut(s) 445, 462
HhaI GCGC 1 cut(s) 412
Hin1II CATG 2 cut(s) 193, 272
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HpaII CCGG 1 cut(s) 50
HphI GGTGA 3 cut(s) 67, 79, 505
Hpy166II GTNNAC 2 cut(s) 195, 239
Hpy188I TCNGA 9 cut(s) 22, 39, 154, 159, 256, 287, 340, 387, 465
Hpy188III TCNNGA 2 cut(s) 67, 360
Hpy8I GTNNAC 2 cut(s) 195, 239
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 2 cut(s) 100, 315
HpyCH4V TGCA 3 cut(s) 232, 449, 509
Hsp92II CATG 2 cut(s) 193, 272
HspAI GCGC 1 cut(s) 410
LmnI GCTCC 1 cut(s) 39
LweI GCATC 1 cut(s) 111
MaeI CTAG 2 cut(s) 8, 140
MaeIII GTNAC 2 cut(s) 163, 310
MbiI CCGCTC 1 cut(s) 367
MboII GAAGA 2 cut(s) 241, 335
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 3 cut(s) 30, 44, 152
MlsI TGGCCA 1 cut(s) 215
MluI ACGCGT 1 cut(s) 454
MluNI TGGCCA 1 cut(s) 215
MmeI TCCRAC 3 cut(s) 45, 153, 194
MnlI CCTC 7 cut(s) 55, 92, 95, 196, 197, 237, 312
Mox20I TGGCCA 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 502
MscI TGGCCA 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 4 cut(s) 217, 323, 328, 380
MvaI CCWGG 4 cut(s) 217, 323, 328, 380
MvnI CGCG 1 cut(s) 456
NcoI CCATGG 1 cut(s) 268
NlaIII CATG 2 cut(s) 193, 272
NlaIV GGNNCC 2 cut(s) 41, 58
NmuCI GTSAC 2 cut(s) 163, 310
NsbI TGCGCA 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 502
PkrI GCNGC 1 cut(s) 369
Ple19I CGATCG 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 4 cut(s) 215, 321, 326, 378
PspGI CCWGG 4 cut(s) 215, 321, 326, 378
PspN4I GGNNCC 2 cut(s) 41, 58
PspPI GGNCC 2 cut(s) 219, 236
PsuI RGATCY 1 cut(s) 56
PvuI CGATCG 1 cut(s) 285
RsaI GTAC 3 cut(s) 260, 277, 469
RsaNI GTAC 3 cut(s) 259, 276, 468
SacI GAGCTC 1 cut(s) 30
SatI GCNGC 1 cut(s) 368
Sau96I GGNCC 2 cut(s) 219, 236
ScaI AGTACT 1 cut(s) 469
ScrFI CCNGG 4 cut(s) 217, 323, 328, 380
SduI GDGCHC 3 cut(s) 30, 44, 152
SetI ASST 8 cut(s) 30, 84, 92, 208, 238, 384, 429, 492
SfaNI GCATC 1 cut(s) 111
SinI GGWCC 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
SsiI CCGC 2 cut(s) 178, 367
SspMI CTAG 2 cut(s) 8, 140
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 4 cut(s) 215, 321, 326, 378
StyI CCWWGG 1 cut(s) 268
TaqI TCGA 2 cut(s) 97, 116
TatI WGTACW 2 cut(s) 258, 467
TauI GCSGC 1 cut(s) 370
TseFI GTSAC 2 cut(s) 163, 310
Tsp45I GTSAC 2 cut(s) 163, 310
TspDTI ATGAA 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 236
XagI CCTNNNNNAGG 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 502
XspI CTAG 2 cut(s) 8, 140
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.