Rh2DG366500

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004873
Physical Location & Seq
Forward (+)
51720204 .. 51723283
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG366500.1

Sequence Viewer

Length: 441 bp
ATGAGCGATGCCCGGGAGAGGTTTTACAATATTCATCTCCAGTATATACAATACATGGTGTCTGCTCTAAAAACTATACAATGTTGTACTCCATATCAGATAAACATGTCCAAGAAATTAAGATTAGTCCGGGGATCATCAAAACAAGGAAACACACTCCTGAACACTGCTGTTTATGCATATGGGTCTCTCCGTTGGACTACTAAGACAAGCTACATATACCATAGCTCATCTTGGATCATTCAGGTTGATGTTTTCACCAACTCTTCCGGAGCATGTGCTGCTTTTATTTCTAATATGGACGACAATAATGACAAAAGAGTAGTATTTCGTTATGTCTCATATCATCTACCAGCATGGTCAGTTAGCATACTGGCTGACTACAAGACTGTAGTATTTAACATAGCAAAGTTGAGATGGCATCTGAAACTAAAGGCATGA

Protein Analysis

146

Amino Acids

16.91

Weight (kDa)

9.74

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHD PF17834 83 - 137 2.9e-18 Beta-sandwich domain in beta galactosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 269
AclWI GGATC 2 cut(s) 142, 245
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 1 cut(s) 18
AflIII ACRYGT 1 cut(s) 105
AluBI AGCT 2 cut(s) 213, 228
AluI AGCT 2 cut(s) 213, 228
Alw26I GTCTC 2 cut(s) 192, 343
AlwI GGATC 2 cut(s) 142, 245
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 281
AsuC2I CCSGG 3 cut(s) 13, 14, 131
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 12
BbvI GCAGC 1 cut(s) 268
BccI CCATC 1 cut(s) 411
BcnI CCSGG 3 cut(s) 13, 14, 131
BcoDI GTCTC 2 cut(s) 192, 343
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 3 cut(s) 13, 14, 131
BmeT110I CYCGRG 1 cut(s) 12
BmrFI CCNGG 3 cut(s) 13, 14, 131
BmsI GCATC 1 cut(s) 430
BpmI CTGGAG 1 cut(s) 23
BpuMI CCSGG 3 cut(s) 13, 14, 131
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 12, 130
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 40, 378
BseAI TCCGGA 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 12, 130
BseLI CCNNNNNNNGG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 40, 378
BseXI GCAGC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 13, 130, 270
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 2 cut(s) 192, 343
Bso31I GGTCTC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 12
Bsp13I TCCGGA 1 cut(s) 269
Bsp143I GATC 2 cut(s) 134, 237
BspEI TCCGGA 1 cut(s) 269
BspPI GGATC 2 cut(s) 142, 245
BspTNI GGTCTC 1 cut(s) 192
BsrI ACTGG 2 cut(s) 40, 378
BssECI CCNNGG 2 cut(s) 12, 130
BssMI GATC 2 cut(s) 134, 237
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 204
BstKTI GATC 2 cut(s) 137, 240
BstMAI GTCTC 2 cut(s) 192, 343
BstMBI GATC 2 cut(s) 134, 237
BstMWI GCNNNNNNNGC 2 cut(s) 176, 281
BstNSI RCATGY 2 cut(s) 109, 279
BstSCI CCNGG 3 cut(s) 11, 12, 129
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
Cfr9I CCCGGG 1 cut(s) 12
Csp6I GTAC 1 cut(s) 87
CviAII CATG 5 cut(s) 55, 106, 276, 357, 438
CviJI RGCY 3 cut(s) 213, 228, 377
CviKI_1 RGCY 3 cut(s) 213, 228, 377
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 1 cut(s) 204
DpnI GATC 2 cut(s) 136, 239
DpnII GATC 2 cut(s) 134, 237
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 181
FaeI CATG 5 cut(s) 58, 109, 279, 360, 441
FatI CATG 5 cut(s) 54, 105, 275, 356, 437
FauNDI CATATG 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 282
GluI GCNGC 1 cut(s) 282
GsuI CTGGAG 1 cut(s) 23
HapII CCGG 3 cut(s) 13, 130, 270
Hin1II CATG 5 cut(s) 58, 109, 279, 360, 441
HpaII CCGG 3 cut(s) 13, 130, 270
HphI GGTGA 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 99, 426
Hpy188III TCNNGA 2 cut(s) 160, 270
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 281
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 5 cut(s) 58, 109, 279, 360, 441
Kpn2I TCCGGA 1 cut(s) 269
Kzo9I GATC 2 cut(s) 134, 237
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 8 cut(s) 26, 53, 143, 173, 230, 283, 359, 366
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 1 cut(s) 430
MalI GATC 2 cut(s) 136, 239
MboI GATC 2 cut(s) 134, 237
MboII GAAGA 1 cut(s) 258
MluCI AATT 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 1 cut(s) 12
Mph1103I ATGCAT 1 cut(s) 181
MroI TCCGGA 1 cut(s) 269
MseI TTAA 2 cut(s) 119, 399
MspI CCGG 3 cut(s) 13, 130, 270
MspR9I CCNGG 3 cut(s) 13, 14, 131
MwoI GCNNNNNNNGC 2 cut(s) 176, 281
NciI CCSGG 3 cut(s) 13, 14, 131
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 134, 237
NlaIII CATG 5 cut(s) 58, 109, 279, 360, 441
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 2 cut(s) 109, 279
PciI ACATGT 1 cut(s) 105
PkrI GCNGC 1 cut(s) 283
PscI ACATGT 1 cut(s) 105
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SaqAI TTAA 2 cut(s) 119, 399
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 2 cut(s) 134, 237
ScrFI CCNGG 3 cut(s) 13, 14, 131
SetI ASST 4 cut(s) 23, 215, 230, 249
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 390
SmaI CCCGGG 1 cut(s) 14
Sse9I AATT 1 cut(s) 116
SspI AATATT 1 cut(s) 31
StyD4I CCNGG 3 cut(s) 11, 12, 129
TaaI ACNGT 1 cut(s) 391
TasI AATT 1 cut(s) 116
TatI WGTACW 1 cut(s) 86
Tru1I TTAA 2 cut(s) 119, 399
Tru9I TTAA 2 cut(s) 119, 399
TscAI CASTG 1 cut(s) 172
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 182
TspMI CCCGGG 1 cut(s) 12
TspRI CASTG 1 cut(s) 172
XceI RCATGY 2 cut(s) 109, 279
XmaI CCCGGG 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.