Rw4G002250
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004873
Physical Location & Seq
Forward (+)
4117058 .. 4118245
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002250.1

Sequence Viewer

Length: 507 bp
ATGGAAGGGGGGAGGAGGATATTTGCAAATCCTCGGCAGTGTAGTGGTCGGAGGGTAGCAGCGTTCATTAACAGCGTTAAGAAGCTTCAACGACGTGAAATCAGTTCCAAGCGTGACCGAGCTATCACTATGAGCGATGCCCGGGAGAGGTTTTACAATATTCATCTCCAGACTACCTGTACCATGGTTCTGCCTTTTCTGCAAAAGAGGTCAAAAATTATTGATATTGTAGCAGCACGTGACATTGTGTTTGCTCTTGCGCAATCTAGTGTTTGGGCAGCTTCATGGAGGATATGCTTTTTGAATGTTAATCCCTGCGAAGTTATAAGAAGCTTGTTTTATAATAAAAACAATGACTCTCCAATCATAATTTCATACGTGAATACCTCCACCCCTCTGCCACTAACAAACAAACACATATATACAATACATGGTGTCTGCTCTAAAAACTATACAATGTTGTACTCCATATCAGATAAACATGTCCAAGAAATTAAGATTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.4

Weight (kDa)

10.12

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 43 - 127 2.9e-21 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 326, 342
Acc16I TGCGCA 1 cut(s) 261
AcvI CACGTG 1 cut(s) 239
AfaI GTAC 2 cut(s) 181, 464
AfiI CCNNNNNNNGG 1 cut(s) 147
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 2 cut(s) 89, 304
AjiI CACGTC 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AluBI AGCT 4 cut(s) 85, 122, 281, 333
AluI AGCT 4 cut(s) 85, 122, 281, 333
Ama87I CYCGRG 1 cut(s) 141
ApeKI GCWGC 3 cut(s) 59, 233, 278
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
AspLEI GCGC 1 cut(s) 262
AsuC2I CCSGG 2 cut(s) 142, 143
AvaI CYCGRG 1 cut(s) 141
BbrPI CACGTG 1 cut(s) 239
BbvI GCAGC 3 cut(s) 71, 245, 290
BcnI CCSGG 2 cut(s) 142, 143
BfaI CTAG 1 cut(s) 267
BisI GCNGC 3 cut(s) 60, 234, 279
BlsI GCNGC 3 cut(s) 61, 235, 280
Bme1390I CCNGG 2 cut(s) 142, 143
BmeT110I CYCGRG 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 142, 143
BmsI GCATC 1 cut(s) 127
BpmI CTGGAG 1 cut(s) 152
BpuMI CCSGG 2 cut(s) 142, 143
BsaAI YACGTR 2 cut(s) 239, 379
BsaJI CCNNGG 3 cut(s) 32, 141, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 32, 141, 183
BseLI CCNNNNNNNGG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 3 cut(s) 71, 245, 290
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 141
Bsp19I CCATGG 1 cut(s) 183
BssECI CCNNGG 3 cut(s) 32, 141, 183
BssT1I CCWWGG 1 cut(s) 183
BstBAI YACGTR 2 cut(s) 239, 379
BstDSI CCRYGG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 140, 141
BstV1I GCAGC 3 cut(s) 71, 245, 290
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 95
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 44
CfoI GCGC 1 cut(s) 262
Cfr9I CCCGGG 1 cut(s) 141
Csp6I GTAC 2 cut(s) 180, 463
CviAII CATG 4 cut(s) 184, 285, 431, 482
CviJI RGCY 4 cut(s) 85, 122, 281, 333
CviKI_1 RGCY 4 cut(s) 85, 122, 281, 333
CviQI GTAC 2 cut(s) 180, 463
Eco130I CCWWGG 1 cut(s) 183
Eco72I CACGTG 1 cut(s) 239
Eco88I CYCGRG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 187, 288, 434, 485
FatI CATG 4 cut(s) 183, 284, 430, 481
Fnu4HI GCNGC 3 cut(s) 60, 234, 279
Fsp4HI GCNGC 3 cut(s) 60, 234, 279
FspBI CTAG 1 cut(s) 267
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 1 cut(s) 261
GluI GCNGC 3 cut(s) 60, 234, 279
GsuI CTGGAG 1 cut(s) 152
HapII CCGG 1 cut(s) 142
HhaI GCGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 187, 288, 434, 485
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HindIII AAGCTT 2 cut(s) 83, 331
HinfI GANTC 1 cut(s) 356
HpaII CCGG 1 cut(s) 142
Hpy188I TCNGA 2 cut(s) 51, 475
Hpy188III TCNNGA 1 cut(s) 169
Hpy99I CGWCG 1 cut(s) 96
HpyCH4IV ACGT 3 cut(s) 94, 238, 378
HpyCH4V TGCA 2 cut(s) 26, 202
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpySE526I ACGT 3 cut(s) 94, 238, 378
Hsp92II CATG 4 cut(s) 187, 288, 434, 485
HspAI GCGC 1 cut(s) 260
LpnPI CCDG 4 cut(s) 155, 182, 190, 328
Lsp1109I GCAGC 3 cut(s) 71, 245, 290
LweI GCATC 1 cut(s) 127
MaeI CTAG 1 cut(s) 267
MaeII ACGT 3 cut(s) 94, 238, 378
MaeIII GTNAC 2 cut(s) 113, 239
MluCI AATT 3 cut(s) 216, 369, 492
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 29
MnlI CCTC 9 cut(s) 6, 9, 42, 45, 141, 201, 282, 397, 405
MseI TTAA 4 cut(s) 69, 78, 309, 495
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 2 cut(s) 142, 143
MwoI GCNNNNNNNGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 142, 143
NcoI CCATGG 1 cut(s) 183
NlaIII CATG 4 cut(s) 187, 288, 434, 485
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 2 cut(s) 113, 239
NsbI TGCGCA 1 cut(s) 261
NspI RCATGY 1 cut(s) 485
PciI ACATGT 1 cut(s) 481
PkrI GCNGC 3 cut(s) 61, 235, 280
PleI GAGTC 1 cut(s) 350
PmaCI CACGTG 1 cut(s) 239
PmlI CACGTG 1 cut(s) 239
PpsI GAGTC 1 cut(s) 350
Ppu21I YACGTR 2 cut(s) 239, 379
PscI ACATGT 1 cut(s) 481
PsiI TTATAA 2 cut(s) 326, 342
PspCI CACGTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 181, 464
RsaNI GTAC 2 cut(s) 180, 463
SaqAI TTAA 4 cut(s) 69, 78, 309, 495
SatI GCNGC 3 cut(s) 60, 234, 279
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 142, 143
SfaNI GCATC 1 cut(s) 127
SmaI CCCGGG 1 cut(s) 143
Sse9I AATT 3 cut(s) 216, 369, 492
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 140, 141
StyI CCWWGG 1 cut(s) 183
TaiI ACGT 3 cut(s) 97, 241, 381
TaqII GACCGA 1 cut(s) 132
TasI AATT 3 cut(s) 216, 369, 492
TatI WGTACW 1 cut(s) 462
Tru1I TTAA 4 cut(s) 69, 78, 309, 495
Tru9I TTAA 4 cut(s) 69, 78, 309, 495
TscAI CASTG 1 cut(s) 44
TseFI GTSAC 2 cut(s) 113, 239
TseI GCWGC 3 cut(s) 59, 233, 278
Tsp45I GTSAC 2 cut(s) 113, 239
TspDTI ATGAA 4 cut(s) 55, 152, 273, 363
TspMI CCCGGG 1 cut(s) 141
TspRI CASTG 1 cut(s) 44
XceI RCATGY 1 cut(s) 485
XmaI CCCGGG 1 cut(s) 141
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.