Rh4CG249100

protein heterodimerization activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002053
Physical Location & Seq
Reverse (-)
49869773 .. 49870749
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG249100.1

Sequence Viewer

Length: 495 bp
ATGGTAGACAATATTGGTGGAAGTGCTCCCAATATCTGTGATCATGGTCAGGGAGATATCAAGACCGAGCAGCCAGACCAATTGCTCCCCATAGCCAACGTTGGGAGGATCATGAAGCAAATCCTGCCACCAAACGCCAAAATCTCAAAGGAAGCCAAGGAAACGATGCAAGAGTGCGTCTCCGAGTTCATCAGCTTCGTCACTGGAGAGGCCTCGCACAAGTGCCAAAAGGAGAAAAGGAAGACGGTGAACGGCGACGACATCTGCTGGGCTCTGGCAACCCTGGGATTCGATAACTACGCCGGCCCGCTGAGGAGGTATTTACACAGGTATAGAGATCAAGAAGGAGCTGAGCACATAGCTAATGATAACAATAATAATGAAGACAAACTGAAGCTGCTGGTTAACAACATCTTGAGTACTGATCAAGAACAAAGGATCATCATGATCATGAACCAATACGATCATCCTCATCATGATATCCAGAAGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000981 GO:0001067 GO:0001654 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0007399 GO:0007423 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010720 GO:0010941 GO:0016070 GO:0016602 GO:0018130 GO:0019216 GO:0019218 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0022008 GO:0022603 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0034097 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043408 GO:0043410 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0044877 GO:0045540 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045676 GO:0045678 GO:0045893 GO:0045935 GO:0045937 GO:0046483 GO:0046532 GO:0046534 GO:0046890 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048699 GO:0048731 GO:0048749 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050810 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051716 GO:0051960 GO:0051962 GO:0060255 GO:0060284 GO:0060548 GO:0062012 GO:0065007 GO:0070013 GO:0070491 GO:0070887 GO:0071310 GO:0071345 GO:0071704 GO:0080090 GO:0090181 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0106118 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1990823 GO:1990830 GO:1990837 GO:2000026 GO:2000027 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 ko05166 map04612 map05152 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

164

Amino Acids

18.61

Weight (kDa)

5.77

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 26 - 92 6.9e-08 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 29 - 92 1.6e-27 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 1 cut(s) 308
AclI AACGTT 1 cut(s) 99
AclWI GGATC 2 cut(s) 116, 446
AcuI CTGAAG 1 cut(s) 413
AfaI GTAC 1 cut(s) 421
AfiI CCNNNNNNNGG 1 cut(s) 102
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 4 cut(s) 195, 350, 362, 397
AluI AGCT 4 cut(s) 195, 350, 362, 397
Alw21I GWGCWC 2 cut(s) 28, 357
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 2 cut(s) 116, 446
AoxI GGCC 2 cut(s) 210, 304
ApeKI GCWGC 2 cut(s) 70, 397
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 259
BanII GRGCYC 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 248, 390
Bbv12I GWGCWC 2 cut(s) 28, 357
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 82, 384
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 3 cut(s) 40, 424, 447
BcoDI GTCTC 1 cut(s) 184
BisI GCNGC 2 cut(s) 71, 398
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 2 cut(s) 72, 399
BmcAI AGTACT 1 cut(s) 421
Bme1390I CCNGG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 305
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 248, 390
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 156, 282, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 208
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 3 cut(s) 156, 282, 283
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 302, 342
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 328
BseXI GCAGC 2 cut(s) 82, 384
BseYI CCCAGC 1 cut(s) 267
BshFI GGCC 2 cut(s) 212, 306
BsiHKAI GWGCWC 2 cut(s) 28, 357
BsiSI CCGG 1 cut(s) 303
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 184
BsmBI CGTCTC 1 cut(s) 184
BsnI GGCC 2 cut(s) 212, 306
Bsp1286I GDGCHC 3 cut(s) 28, 274, 357
Bsp143I GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
Bsp1720I GCTNAGC 1 cut(s) 351
BspACI CCGC 1 cut(s) 308
BspANI GGCC 2 cut(s) 212, 306
BspCNI CTCAG 2 cut(s) 303, 343
BspHI TCATGA 4 cut(s) 111, 444, 450, 475
BspPI GGATC 2 cut(s) 116, 446
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 156, 282, 283
BssMI GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 304, 308
BstDEI CTNAG 2 cut(s) 311, 351
BstF5I GGATG 1 cut(s) 466
BstKTI GATC 7 cut(s) 43, 111, 340, 427, 441, 450, 466
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 82, 384
BstV2I GAAGAC 2 cut(s) 248, 390
BsuRI GGCC 2 cut(s) 212, 306
BtsCI GGATG 1 cut(s) 466
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 2 cut(s) 304, 308
CciI TCATGA 4 cut(s) 111, 444, 450, 475
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 420
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 5 cut(s) 44, 112, 445, 451, 476
CviQI GTAC 1 cut(s) 420
DdeI CTNAG 2 cut(s) 311, 351
DpnI GATC 7 cut(s) 42, 110, 339, 426, 440, 449, 465
DpnII GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
Eco130I CCWWGG 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 274
Eco32I GATATC 2 cut(s) 58, 481
Eco57I CTGAAG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 282
EcoRV GATATC 2 cut(s) 58, 481
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 5 cut(s) 47, 115, 448, 454, 479
FaiI YATR 8 cut(s) 45, 92, 113, 333, 359, 446, 452, 477
FatI CATG 5 cut(s) 43, 111, 444, 450, 475
FauI CCCGC 1 cut(s) 315
FbaI TGATCA 3 cut(s) 40, 424, 447
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 71, 398
FokI GGATG 1 cut(s) 453
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 2 cut(s) 71, 398
GluI GCNGC 2 cut(s) 71, 398
GsaI CCCAGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 2 cut(s) 212, 306
HapII CCGG 1 cut(s) 303
HgaI GACGC 1 cut(s) 166
Hin1II CATG 5 cut(s) 47, 115, 448, 454, 479
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 1 cut(s) 288
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 1 cut(s) 303
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 3 cut(s) 7, 250, 406
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 9 cut(s) 61, 112, 341, 415, 428, 445, 451, 476, 484
Hpy8I GTNNAC 3 cut(s) 7, 250, 406
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 2 cut(s) 311, 351
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 5 cut(s) 47, 115, 448, 454, 479
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 3 cut(s) 40, 424, 447
KspAI GTTAAC 1 cut(s) 406
Kzo9I GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
LmnI GCTCC 3 cut(s) 31, 90, 347
Lsp1109I GCAGC 2 cut(s) 82, 384
LweI GCATC 1 cut(s) 156
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 199
MalI GATC 7 cut(s) 42, 110, 339, 426, 440, 449, 465
MboI GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
MboII GAAGA 2 cut(s) 253, 395
MfeI CAATTG 1 cut(s) 80
MhlI GDGCHC 3 cut(s) 28, 274, 357
MluCI AATT 1 cut(s) 80
MnlI CCTC 6 cut(s) 99, 202, 223, 306, 309, 480
MroNI GCCGGC 1 cut(s) 302
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 284
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 124
NaeI GCCGGC 1 cut(s) 304
NdeII GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 5 cut(s) 47, 115, 448, 454, 479
NmuCI GTSAC 1 cut(s) 199
PagI TCATGA 4 cut(s) 111, 444, 450, 475
PasI CCCWGGG 1 cut(s) 283
PceI AGGCCT 1 cut(s) 212
PdiI GCCGGC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 288
PkrI GCNGC 2 cut(s) 72, 399
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 1 cut(s) 282
PspPI GGNCC 1 cut(s) 305
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 2 cut(s) 71, 398
Sau3AI GATC 7 cut(s) 40, 108, 337, 424, 438, 447, 463
Sau96I GGNCC 1 cut(s) 305
ScaI AGTACT 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 3 cut(s) 28, 274, 357
SetI ASST 8 cut(s) 102, 197, 320, 332, 352, 364, 399, 494
SfaNI GCATC 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 415
SmoI CTYRAG 1 cut(s) 415
Sse9I AATT 1 cut(s) 80
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 1 cut(s) 308
SspI AATATT 1 cut(s) 13
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 291
TaqII GACCGA 1 cut(s) 80
TasI AATT 1 cut(s) 80
TatI WGTACW 1 cut(s) 419
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 70, 397
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 4 cut(s) 128, 178, 396, 467
TspRI CASTG 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.