Rh4DG156000

WD repeat-containing protein C2A9.03-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002709
Physical Location & Seq
Reverse (-)
31231466 .. 31238514
7049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG156000.1

Sequence Viewer

Length: 744 bp
ATGGCTTTTGAGCAGCCTCATCAGTTTGAATATATGGCGGATGAGGGTGACATGTCTGATTTCGTTGAGGACGCAGATGTGGATGCCGATGAAGAAAATCATGGTAGAGATATGGTTCTTGACGAATACGACATGCGTCTTGATAAAGAAGGAGTGAGTTTTTGTACGCGAACCACATACGATGACAATGCCATCACAAATGCTGTTGAAATATATGATAGCCTGGGGGGTGGAATGCATTTCATGGCATCCAATAATGATTGTGGTATAAGAGAATATGACATGGAGAGATTTCAGCTTCTGAATCACTTTCGATTCCCCTGGCCAGTGAATCATACATCAATAAGCCCAGATCGTAGGGTTGTTGCCGTAGTTGGAGATCACGTGGATGGATTGCTTATGGATTCTCAGAATGGAAAGACTATTGCTAAGAAAACACCTGCAGGTAAAATAGGAGGCATTGCTGTGGTTGATTTGGATCGAGAGAGGAAAGGGATGGAGTCTAGGATGGATCAGTATGAGCTGATGGAGCAGATCGGCCGGGGAGCTTTGGGAGCCGCGATTTTGGTTCATCACAAGGCTGAGAAGAAGAAGTATGTGTTGAAGAAGATCCGGCTTGCGAGGCAGACGGAGCGGTGCTGGAGATCTGCTCATCAAGAGATGGCTCTTATTGCTCGGATTCAGCACCCGTACATTGTGGAATTTAAGGAACCCTGGGTTGAGAAGTTCAGATACTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

247

Amino Acids

28.49

Weight (kDa)

5.31

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 173 - 241 4.5e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
Acc36I ACCTGC 2 cut(s) 434, 448
AccBSI CCGCTC 1 cut(s) 634
AccII CGCG 2 cut(s) 169, 560
AciI CCGC 3 cut(s) 38, 558, 634
AclWI GGATC 3 cut(s) 486, 519, 604
AcoI YGGCCR 2 cut(s) 323, 538
AcsI RAATTY 1 cut(s) 701
AcvI CACGTG 1 cut(s) 385
AfaI GTAC 2 cut(s) 166, 692
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 4 cut(s) 29, 209, 604, 739
AjnI CCWGG 3 cut(s) 222, 320, 713
AluBI AGCT 3 cut(s) 298, 523, 548
AluI AGCT 3 cut(s) 298, 523, 548
AlwI GGATC 3 cut(s) 486, 519, 604
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 2 cut(s) 323, 538
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 701
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 1 cut(s) 59
BalI TGGCCA 1 cut(s) 325
BarI GAAGNNNNNNTAC 1 cut(s) 716
BbrPI CACGTG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 25
BccI CCATC 6 cut(s) 200, 383, 490, 502, 520, 655
BceAI ACGGC 1 cut(s) 353
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 3 cut(s) 224, 322, 715
BcnI CCSGG 1 cut(s) 542
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 2 cut(s) 434, 448
BglII AGATCT 1 cut(s) 644
BisI GCNGC 2 cut(s) 14, 558
BlsI GCNGC 2 cut(s) 15, 559
Bme1390I CCNGG 4 cut(s) 224, 322, 542, 715
BmiI GGNNCC 2 cut(s) 556, 711
BmrFI CCNGG 4 cut(s) 224, 322, 542, 715
BmsI GCATC 2 cut(s) 73, 257
BoxI GACNNNNGTC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 1 cut(s) 661
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 477
BsaJI CCNNGG 5 cut(s) 223, 320, 541, 713, 714
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 477
BseBI CCWGG 3 cut(s) 224, 322, 715
BseDI CCNNGG 5 cut(s) 223, 320, 541, 713, 714
BseGI GGATG 6 cut(s) 46, 88, 248, 394, 501, 513
BseJI GATNNNNATC 1 cut(s) 477
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 2 cut(s) 422, 573
BseNI ACTGG 1 cut(s) 326
BseX3I CGGCCG 1 cut(s) 538
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 169, 560
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 325, 540
BsiEI CGRYCG 1 cut(s) 541
BsiSI CCGG 2 cut(s) 541, 613
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 2 cut(s) 325, 540
Bsp143I GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
BspACI CCGC 3 cut(s) 38, 558, 634
BspANI GGCC 2 cut(s) 325, 540
BspCNI CTCAG 2 cut(s) 421, 574
BspFNI CGCG 2 cut(s) 169, 560
BspLI GGNNCC 2 cut(s) 556, 711
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 2 cut(s) 434, 448
BspPI GGATC 3 cut(s) 486, 519, 604
BsrBI CCGCTC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 5 cut(s) 223, 320, 541, 713, 714
BssMI GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
Bst2UI CCWGG 3 cut(s) 224, 322, 715
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 3 cut(s) 408, 429, 582
BstF5I GGATG 6 cut(s) 46, 88, 248, 394, 501, 513
BstFNI CGCG 2 cut(s) 169, 560
BstKTI GATC 7 cut(s) 355, 382, 481, 514, 537, 612, 647
BstMBI GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
BstMCI CGRYCG 1 cut(s) 541
BstMWI GCNNNNNNNGC 5 cut(s) 529, 554, 622, 631, 671
BstNI CCWGG 3 cut(s) 224, 322, 715
BstNSI RCATGY 2 cut(s) 55, 136
BstPAI GACNNNNGTC 1 cut(s) 135
BstSCI CCNGG 4 cut(s) 222, 320, 540, 713
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 2 cut(s) 169, 560
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 609, 644
BstYI RGATCY 2 cut(s) 609, 644
BstZI CGGCCG 1 cut(s) 538
BsuRI GGCC 2 cut(s) 325, 540
BtsCI GGATG 6 cut(s) 46, 88, 248, 394, 501, 513
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 2 cut(s) 434, 448
Cac8I GCNNGC 1 cut(s) 618
CaiI CAGNNNCTG 1 cut(s) 301
CseI GACGC 2 cut(s) 80, 125
Csp6I GTAC 2 cut(s) 165, 691
CviAII CATG 5 cut(s) 52, 101, 133, 244, 283
CviQI GTAC 2 cut(s) 165, 691
DdeI CTNAG 3 cut(s) 408, 429, 582
DpnI GATC 7 cut(s) 354, 381, 480, 513, 536, 611, 646
DpnII GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
EaeI YGGCCR 2 cut(s) 323, 538
EagI CGGCCG 1 cut(s) 538
EciI GGCGGA 1 cut(s) 53
EclXI CGGCCG 1 cut(s) 538
Eco52I CGGCCG 1 cut(s) 538
Eco72I CACGTG 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 222, 320, 713
EcoT22I ATGCAT 1 cut(s) 240
FaeI CATG 5 cut(s) 55, 104, 136, 247, 286
FatI CATG 5 cut(s) 51, 100, 132, 243, 282
Fnu4HI GCNGC 2 cut(s) 14, 558
FokI GGATG 6 cut(s) 53, 95, 235, 401, 508, 520
Fsp4HI GCNGC 2 cut(s) 14, 558
FspBI CTAG 1 cut(s) 504
GluI GCNGC 2 cut(s) 14, 558
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 2 cut(s) 325, 540
HapII CCGG 2 cut(s) 541, 613
HgaI GACGC 2 cut(s) 80, 125
Hin1II CATG 5 cut(s) 55, 104, 136, 247, 286
HinfI GANTC 6 cut(s) 304, 315, 331, 404, 500, 679
HpaII CCGG 2 cut(s) 541, 613
HphI GGTGA 1 cut(s) 59
Hpy188I TCNGA 5 cut(s) 58, 303, 411, 678, 731
Hpy188III TCNNGA 4 cut(s) 119, 140, 482, 656
HpyAV CCTTC 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 238, 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 529, 554, 622, 631, 671
HpyF3I CTNAG 3 cut(s) 408, 429, 582
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 5 cut(s) 55, 104, 136, 247, 286
Kzo9I GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
LmnI GCTCC 4 cut(s) 529, 545, 554, 631
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 2 cut(s) 73, 257
MaeI CTAG 1 cut(s) 504
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 47
MalI GATC 7 cut(s) 354, 381, 480, 513, 536, 611, 646
MbiI CCGCTC 1 cut(s) 634
MboI GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
MboII GAAGA 5 cut(s) 104, 598, 601, 616, 619
MflI RGATCY 2 cut(s) 609, 644
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 2 cut(s) 701, 739
MluNI TGGCCA 1 cut(s) 325
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 6 cut(s) 27, 37, 61, 449, 480, 615
Mox20I TGGCCA 1 cut(s) 325
Mph1103I ATGCAT 1 cut(s) 240
MscI TGGCCA 1 cut(s) 325
MseI TTAA 1 cut(s) 705
MslI CAYNNNNRTG 2 cut(s) 387, 464
Msp20I TGGCCA 1 cut(s) 325
MspI CCGG 2 cut(s) 541, 613
MspR9I CCNGG 4 cut(s) 224, 322, 542, 715
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 3 cut(s) 224, 322, 715
MvnI CGCG 2 cut(s) 169, 560
MwoI GCNNNNNNNGC 5 cut(s) 529, 554, 622, 631, 671
NciI CCSGG 1 cut(s) 542
NdeII GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
NlaIII CATG 5 cut(s) 55, 104, 136, 247, 286
NlaIV GGNNCC 2 cut(s) 556, 711
NmuCI GTSAC 1 cut(s) 47
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 2 cut(s) 55, 136
PaqCI CACCTGC 1 cut(s) 448
PasI CCCWGGG 1 cut(s) 714
PciI ACATGT 1 cut(s) 51
PctI GAATGC 1 cut(s) 240
PfeI GAWTC 5 cut(s) 304, 315, 331, 404, 679
PkrI GCNGC 2 cut(s) 15, 559
PleI GAGTC 1 cut(s) 508
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 385
PscI ACATGT 1 cut(s) 51
PshAI GACNNNNGTC 1 cut(s) 135
Psp6I CCWGG 3 cut(s) 222, 320, 713
PspCI CACGTG 1 cut(s) 385
PspGI CCWGG 3 cut(s) 222, 320, 713
PspN4I GGNNCC 2 cut(s) 556, 711
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 2 cut(s) 609, 644
RsaI GTAC 2 cut(s) 166, 692
RsaNI GTAC 2 cut(s) 165, 691
RseI CAYNNNNRTG 2 cut(s) 387, 464
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 2 cut(s) 14, 558
Sau3AI GATC 7 cut(s) 352, 379, 478, 511, 534, 609, 644
SbfI CCTGCAGG 1 cut(s) 445
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 4 cut(s) 224, 322, 542, 715
SdaI CCTGCAGG 1 cut(s) 445
SetI ASST 6 cut(s) 300, 387, 442, 448, 525, 550
SfaNI GCATC 2 cut(s) 73, 257
SfcI CTRYAG 1 cut(s) 441
SmiMI CAYNNNNRTG 2 cut(s) 387, 464
Sse8387I CCTGCAGG 1 cut(s) 445
Sse9I AATT 2 cut(s) 701, 739
SsiI CCGC 3 cut(s) 38, 558, 634
SspMI CTAG 1 cut(s) 504
StyD4I CCNGG 4 cut(s) 222, 320, 540, 713
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 313, 481
TasI AATT 2 cut(s) 701, 739
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 5 cut(s) 304, 315, 331, 404, 679
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 3 cut(s) 105, 232, 560
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 1 cut(s) 333
XapI RAATTY 1 cut(s) 701
XceI RCATGY 2 cut(s) 55, 136
XspI CTAG 1 cut(s) 504
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.