Rh5DG072800

3-phosphoinositide-dependent protein kinase-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003594
Physical Location & Seq
Reverse (-)
6340938 .. 6341742
805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG072800.1

Sequence Viewer

Length: 726 bp
ATGAAGAGGGAGGCAGGAGAGAGACAAAGCAATGGGTCGTCATCATCTTCATCCGAAGTACCTCTTATCAATTCTCAGTCCTCCTCCCATCCAACCGAACAGCTTCAATCCGGTTCACAAGTCGAGATGGGTTCCGGAGGCCCCGACCCAGTAACCTGGCAACAAATTGAGAATTCCGGGACCTACCTTGTCTGCTCCATGTACGAGGAGGCTGCCTCATTAGCTTTCTCTATTCTGAAACGTCTTTCTCAACATACCGAGGATGATGATGATGACCTGTATGACATGTTGGAATCGGCAGAAGGGCATTCTCTTGAATACAATGCAGATTATACAAAAAGACTTGATGGGCATTTTGTTTTGGGCATTGATAAGTATCTTGTAGTAGCTGAGGTGTATACTTTGAATGTGCTGGGAACCATTTTGAATAGTGTTGACCTTGCTAACTCTTGGGTCGAGAATGCTAATATACCTGAGGACGGAAGACAGGTAAACAATTACATACATCTTATGTTGGAGACAGCACCACGAAAAATTGATTTAGATGGTTATATACTACTATACCACACAACGTACCTGGTGGATATTGACAGCGAGAAGGTTGGAAGAGTCTTGAAGCTCGACTCCATCAGGGGCGGTAACGCTTGGAAAGGCATGGACGTCCTGATCTTCGACACGTGGCATTGGTCAACAAGTGGATGCATTTTGAGTTCTCAGAAAGTATAG

Protein Analysis

241

Amino Acids

26.8

Weight (kDa)

4.55

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 185 - 234 4.1e-06 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 663
AccI GTMKAC 1 cut(s) 398
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 1 cut(s) 636
AcsI RAATTY 1 cut(s) 172
AcvI CACGTG 1 cut(s) 678
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 3 cut(s) 60, 203, 575
AfiI CCNNNNNNNGG 1 cut(s) 479
AflIII ACRYGT 2 cut(s) 285, 675
AgsI TTSAA 5 cut(s) 107, 317, 406, 427, 616
AjnI CCWGG 2 cut(s) 155, 576
AluBI AGCT 4 cut(s) 103, 224, 389, 619
AluI AGCT 4 cut(s) 103, 224, 389, 619
Alw26I GTCTC 2 cut(s) 16, 512
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 140, 180
AsuC2I CCSGG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 180
AxyI CCTNAGG 1 cut(s) 474
BbrPI CACGTG 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 490
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 199
BccI CCATC 5 cut(s) 96, 121, 341, 539, 635
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 2 cut(s) 157, 578
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 16, 512
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 157, 178, 578
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 140, 180
BmiI GGNNCC 4 cut(s) 133, 142, 181, 418
BmrFI CCNGG 3 cut(s) 157, 178, 578
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 490
BplI GAGNNNNNCTC 2 cut(s) 200, 232
Bpu10I CCTNAGC 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 660
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 2 cut(s) 110, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 149
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 2 cut(s) 157, 578
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 4 cut(s) 50, 88, 268, 704
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 3 cut(s) 89, 381, 465
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 2 cut(s) 73, 221
BseXI GCAGC 1 cut(s) 199
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 1 cut(s) 141
BsiSI CCGG 3 cut(s) 111, 135, 177
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 16, 512
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 2 cut(s) 307, 466
BsnI GGCC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 1 cut(s) 666
BspACI CCGC 1 cut(s) 636
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 3 cut(s) 88, 382, 466
BspEI TCCGGA 1 cut(s) 134
BspLI GGNNCC 4 cut(s) 133, 142, 181, 418
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 666
BssNAI GTATAC 1 cut(s) 399
BssNI GRCGYC 1 cut(s) 660
Bst1107I GTATAC 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 157, 578
Bst6I CTCTTC 1 cut(s) 601
BstACI GRCGYC 1 cut(s) 660
BstBAI YACGTR 1 cut(s) 678
BstDEI CTNAG 4 cut(s) 75, 390, 474, 714
BstF5I GGATG 4 cut(s) 50, 88, 268, 704
BstKTI GATC 1 cut(s) 669
BstMAI GTCTC 2 cut(s) 16, 512
BstMBI GATC 1 cut(s) 666
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNI CCWGG 2 cut(s) 157, 578
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 3 cut(s) 155, 176, 576
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 156
BstZ17I GTATAC 1 cut(s) 399
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 1 cut(s) 141
BtsCI GGATG 4 cut(s) 50, 88, 268, 704
Cfr13I GGNCC 2 cut(s) 140, 180
CsiI ACCWGGT 1 cut(s) 576
Csp6I GTAC 3 cut(s) 59, 202, 574
CviAII CATG 3 cut(s) 199, 286, 655
CviJI RGCY 6 cut(s) 103, 141, 212, 224, 389, 619
CviKI_1 RGCY 6 cut(s) 103, 141, 212, 224, 389, 619
CviQI GTAC 3 cut(s) 59, 202, 574
DdeI CTNAG 4 cut(s) 75, 390, 474, 714
DpnI GATC 1 cut(s) 668
DpnII GATC 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 180
Eco72I CACGTG 1 cut(s) 678
Eco81I CCTNAGG 1 cut(s) 474
EcoO109I RGGNCCY 2 cut(s) 140, 180
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 155, 576
EcoT22I ATGCAT 1 cut(s) 704
FaeI CATG 3 cut(s) 202, 289, 658
FalI AAGNNNNNCTT 2 cut(s) 48, 80
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 198, 285, 654
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 4 cut(s) 37, 75, 275, 711
Fsp4HI GCNGC 1 cut(s) 213
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 416
HaeIII GGCC 1 cut(s) 141
HapII CCGG 3 cut(s) 111, 135, 177
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 3 cut(s) 202, 289, 658
HincII GTYRAC 2 cut(s) 436, 690
HindII GTYRAC 2 cut(s) 436, 690
HinfI GANTC 3 cut(s) 293, 609, 623
HpaII CCGG 3 cut(s) 111, 135, 177
Hpy166II GTNNAC 5 cut(s) 116, 399, 436, 493, 690
Hpy188I TCNGA 3 cut(s) 55, 237, 717
Hpy188III TCNNGA 6 cut(s) 124, 135, 314, 457, 613, 664
Hpy8I GTNNAC 5 cut(s) 116, 399, 436, 493, 690
HpyAV CCTTC 2 cut(s) 296, 592
HpyCH4IV ACGT 4 cut(s) 241, 572, 660, 677
HpyCH4V TGCA 2 cut(s) 326, 702
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 4 cut(s) 75, 390, 474, 714
HpySE526I ACGT 4 cut(s) 241, 572, 660, 677
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 3 cut(s) 202, 289, 658
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 1 cut(s) 666
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 689
MabI ACCWGGT 1 cut(s) 576
MaeII ACGT 4 cut(s) 241, 572, 660, 677
MaeIII GTNAC 2 cut(s) 151, 638
MalI GATC 1 cut(s) 668
MboI GATC 1 cut(s) 666
MboII GAAGA 5 cut(s) 16, 39, 495, 618, 661
MluCI AATT 5 cut(s) 70, 165, 172, 496, 534
MlyI GAGTC 2 cut(s) 617, 618
MmeI TCCRAC 4 cut(s) 116, 270, 495, 583
Mph1103I ATGCAT 1 cut(s) 704
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 102
MspI CCGG 3 cut(s) 111, 135, 177
MspR9I CCNGG 3 cut(s) 157, 178, 578
Mva1269I GAATGC 2 cut(s) 307, 466
MvaI CCWGG 2 cut(s) 157, 578
MwoI GCNNNNNNNGC 1 cut(s) 221
NciI CCSGG 1 cut(s) 178
NdeII GATC 1 cut(s) 666
NlaIII CATG 3 cut(s) 202, 289, 658
NlaIV GGNNCC 4 cut(s) 133, 142, 181, 418
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PctI GAATGC 2 cut(s) 307, 466
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 1 cut(s) 293
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 2 cut(s) 617, 617
PmaCI CACGTG 1 cut(s) 678
PmlI CACGTG 1 cut(s) 678
PpsI GAGTC 2 cut(s) 617, 617
Ppu21I YACGTR 1 cut(s) 678
PpuMI RGGWCCY 1 cut(s) 180
PscI ACATGT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 155, 576
PspCI CACGTG 1 cut(s) 678
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 2 cut(s) 155, 576
PspN4I GGNNCC 4 cut(s) 133, 142, 181, 418
PspPI GGNCC 2 cut(s) 140, 180
PspPPI RGGWCCY 1 cut(s) 180
RsaI GTAC 3 cut(s) 60, 203, 575
RsaNI GTAC 3 cut(s) 59, 202, 574
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 666
Sau96I GGNCC 2 cut(s) 140, 180
SchI GAGTC 2 cut(s) 617, 618
ScrFI CCNGG 3 cut(s) 157, 178, 578
SexAI ACCWGGT 1 cut(s) 576
SfaNI GCATC 1 cut(s) 689
SinI GGWCC 1 cut(s) 180
Sse9I AATT 5 cut(s) 70, 165, 172, 496, 534
SsiI CCGC 1 cut(s) 636
StyD4I CCNGG 3 cut(s) 155, 176, 576
TaiI ACGT 4 cut(s) 244, 575, 663, 680
TaqI TCGA 4 cut(s) 123, 456, 621, 672
TasI AATT 5 cut(s) 70, 165, 172, 496, 534
TfiI GAWTC 1 cut(s) 293
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 17, 39
TspGWI ACGGA 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 1 cut(s) 172
XceI RCATGY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 102
ZraI GACGTC 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.