Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0004915

Physical Location & Seq

Genomic Coordinates Reverse (-)

56569798 .. 56573249

Gene Structure 3452 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G028860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGATAGGTTCCATCTTACCCTTACCAGCTCCTCCTGCAAAATCAGTTGCCACACATACCAAGGCTATTGGTATCATACCTCCTCCTCCAGGAATCAAAGTCGTCATTGATAAAACTGCTCAGCTCGTGGCAAAGAACGGACCCCAATTTGAACAGGTCATAGCTAAGAATCTAGACATAGCAAAATCAAATCTAAGCTTTTTGATTTCCTCAAATCCCTACAATGCATATTATAAGCGCCGGGTGTCTGAATTCCTTGCCCAGCCTGATCAGTCTTATGAAGAGCCAGCTCCAGTTGTAGATGTTGAAGCTGGAGCACCAAAACCTGATTCTCCCTCCCAGCTTAGACCTGAATGCAAAGTGCCTGAACCATCAGAACCTGAAGAGTTTACTGTTCGGCTTCCTGAAGGAATTTGTGGAGAAGAACTAGATGTTATTAAACTCACAGCCCAGTTTGTAGCTCGGAATGGGAAATCGTTTTTGACAGGATTGAGAAGCAGAGAGATAAACAACCCCCTGTTCTATTTTCTCAAGCCTAATCATAGCATGTTCAACTTTTTCACTTCTCTGGCAGATGCATATTCAAGTGTGGCAGACATGTCTACTGTGCTAGAACGGTGTGTGCATCGGCTGGAGTGGGAGGCTTATCAAAAGAAGGCAAAGCAGAAGGCCGAAGATGAGATTGAGCAGGAAAGGTTACAAATGGCTGCAATTGATTGGCACGATAATGTCTTGGTCAAAGTCATAGACTTTGATGATGTTGAGGATGACTGTTTACCTCCTCCACTGAGCATCGAAGAGGTTATAAGGGGAAGCAAAGTTGTTATGGAAGCAAATGATAATGTCGAAATGGATATGGATGAAGAAGAGATGCAACTTGTTGAAGATGGAAAAAGGGTAACAGAGGTGGATCCAGAACAACCACCAATGAGAATTGTGAAGAACTGGAAGAGACCTGAGGAGAGGATGATCCAATCCAGTGCTGAAAGGGACATGACGATTTCCCCTATTACTGGGGAGCTTGTCCCTAACAATGAGATGTCTGAGCACATGAAGATTTCCCTCATTGATCCAAGATACAGAGAACAGAAAGAAAGGATGTTTGCTAAGATTCGGGAGACTAGTCTTGTTCTTGCACAGGACGATGAGATCTCAAGAAACATAGTGAACCTTGCGCGGACCCGTCCTGATATTTTTGGTTCCACCGAGGAGGAAGTTTCAAATGCTGTCAAGGCTGAGATTCAAAATCAAGCGATATCACTACGTGCCTTGTCAGGCCAAAATGCTCATTTTGGGTCCAAACAATCACAGAGTATCAATGGAGAGGATAAGAATGATGCTGTGAACAATGAGCTAAGGAACTTTCCTGGTCCAGCAAGACCTGACATGCCTTCAGTTCGACCTCAACCTCCACCAACTGGCCTTCTTCCTCGTGTTCCTCTAAGCACGGTTCAGTGTTCAGCTCCTAATAGCATTGGCCTCCCAATACCTCCTCCAGTTGTCCAATTTCCATCCGTTGGAGCTCCTCTGCCACCTCAATCAATATTTACGACTGCAACTAATATTCCTGCACCATCTCCACCTGTAGCTCAGTTTACACCTATGCAAGTTCCCCAAAGGAATTATATTCCTCCACCTTCAGTGCATATGATGCCCCTGCCTCCACCACCACCTTTACCTCAAGAAATGCATCTACCACCACCACCACCACCACCACCTCCAGTTGCAGAGGAGGAACCCAAGCCAAAGCGACAGAAGCTCGATGATTCAATGCTTATTCCAGAAGATAAACTTCTGGCACAGCATCCGGGACCAATCACGGTGCAGTCTTTATCTGAAACTGTTGGCAGTCTGAAAGAGAAAATTTCTGCAGAAGTCCAACTTCCGGCAAACAAACAGAAATTGAGTGGAAAATCAGGCTTTCTCAAGGATAATTTTTCACTTGCATATTACAATGTTGGCGCAGGACAAACACTCGCTCTTTCAATAAGAGAGCGCGGTGGAAGGAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000381 GO:0000389 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005686 GO:0006139 GO:0006376 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0010468 GO:0016043 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0031323 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048024 GO:0050684 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065003 GO:0065007 GO:0071004 GO:0071010 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1902494 GO:1903311 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

PRP21_like_P Surp ubiquitin

Protein Analysis

671

Amino Acids

74.36

Weight (kDa)

5.27

Isoelectric Point (pI)

60.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Surp	PF01805	35 - 85	9.9e-14	Surp module
Surp	PF01805	143 - 194	1.1e-16	Surp module
PRP21_like_P	PF12230	198 - 393	2.2e-46	Pre-mRNA splicing factor PRP21 like protein
ubiquitin	PF00240	611 - 666	7.9e-08	Ubiquitin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004915)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06890
malus_domestica	MD10G1329400.v1.1
pyrus_communis	pycom05g32220 pycom10g27920
rosa_chinensis	RchiOBHm_Chr7g0191091
rosa_laevigata	RLG00000004475
rosa_multiflora	Rmu_sc0005151.1_g000002 Rmu_sc0005657.1_g000002 Rmu_sc0005903.1_g000003 Rmu_sc0005903.1_g000004 Rmu_sc0005903.1_g000005 Rmu_sc0013941.1_g000002 Rmu_sc0034959.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264050 Rroxscaffold_5G00351920
rosa_rugosa	Rorug01G0317000 Rorug01G0317100
rosa_samantha	Rh1AG145900 Rh1AG325300 Rh3BG312100 Rh4AG053700 Rh4BG051600 Rh4DG247700 Rh7AG116000 Rh7BG117500 Rh7CG120900 Rh7DG298100
rosa_wichuraiana	Rw1G028860 Rw7G010070 Rw7G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	234, 806
AccB7I	CCANNNNNTGG	2 cut(s)	1418, 1517
AccI	GTMKAC	1 cut(s)	602
AccII	CGCG	2 cut(s)	1177, 1998
AciI	CCGC	2 cut(s)	1177, 1998
AclWI	GGATC	4 cut(s)	905, 918, 964, 1064
AcsI	RAATTY	3 cut(s)	251, 411, 1863
AcuI	CTGAAG	4 cut(s)	402, 426, 1377, 1623
AdeI	CACNNNGTG	1 cut(s)	1265
AfiI	CCNNNNNNNGG	5 cut(s)	89, 1013, 1418, 1517, 1885
AflIII	ACRYGT	1 cut(s)	597
AgsI	TTSAA	9 cut(s)	152, 308, 553, 585, 884, 1221, 1244, 1770, 1986
AhlI	ACTAGT	1 cut(s)	1121
AjnI	CCWGG	2 cut(s)	88, 1366
AjuI	GAANNNNNNNTTGG	2 cut(s)	313, 345
Alw21I	GWGCWC	3 cut(s)	319, 1050, 1525
Alw26I	GTCTC	2 cut(s)	946, 1112
AlwI	GGATC	4 cut(s)	905, 918, 964, 1064
AlwNI	CAGNNNCTG	1 cut(s)	380
AoxI	GGCC	4 cut(s)	669, 1276, 1420, 1477
ApeKI	GCWGC	1 cut(s)	707
ApoI	RAATTY	3 cut(s)	251, 411, 1863
AspLEI	GCGC	4 cut(s)	240, 1177, 1964, 1998
AspS9I	GGNCC	5 cut(s)	140, 1179, 1296, 1370, 1811
AsuC2I	CCSGG	2 cut(s)	242, 1809
AvaII	GGWCC	5 cut(s)	140, 1179, 1296, 1370, 1811
AxyI	CCTNAGG	1 cut(s)	957
BamHI	GGATCC	1 cut(s)	910
BanII	GRGCYC	1 cut(s)	1525
BauI	CACGAG	2 cut(s)	125, 1431
Bbv12I	GWGCWC	3 cut(s)	319, 1050, 1525
BbvI	GCAGC	1 cut(s)	694
BccI	CCATC	5 cut(s)	20, 379, 881, 1519, 1582
BciT130I	CCWGG	2 cut(s)	90, 1368
BclI	TGATCA	1 cut(s)	268
BcnI	CCSGG	2 cut(s)	242, 1809
BcoDI	GTCTC	2 cut(s)	946, 1112
BcuI	ACTAGT	1 cut(s)	1121
BfaI	CTAG	4 cut(s)	173, 428, 611, 1122
BfmI	CTRYAG	2 cut(s)	1584, 1869
BfoI	RGCGCY	1 cut(s)	241
BglII	AGATCT	1 cut(s)	1149
BisI	GCNGC	1 cut(s)	708
BlpI	GCTNAGC	1 cut(s)	120
BlsI	GCNGC	1 cut(s)	709
Bme1390I	CCNGG	4 cut(s)	90, 242, 1368, 1809
Bme18I	GGWCC	5 cut(s)	140, 1179, 1296, 1370, 1811
BmgT120I	GGNCC	5 cut(s)	140, 1179, 1296, 1370, 1811
BmiI	GGNNCC	8 cut(s)	10, 142, 912, 1181, 1201, 1297, 1737, 1812
BmrFI	CCNGG	4 cut(s)	90, 242, 1368, 1809
BmrI	ACTGGG	2 cut(s)	445, 1023
BmsI	GCATC	8 cut(s)	565, 634, 801, 861, 1327, 1641, 1699, 1813
BmuI	ACTGGG	2 cut(s)	445, 1023
BpmI	CTGGAG	6 cut(s)	72, 276, 333, 653, 1479, 1704
Bpu10I	CCTNAGC	1 cut(s)	1355
Bpu1102I	GCTNAGC	1 cut(s)	120
BpuEI	CTTGAG	4 cut(s)	515, 1138, 1665, 1910
BpuMI	CCSGG	2 cut(s)	242, 1809
BsaAI	YACGTR	1 cut(s)	1265
BsaI	GGTCTC	1 cut(s)	946
BsaJI	CCNNGG	2 cut(s)	60, 1206
Bsc4I	CCNNNNNNNGG	5 cut(s)	89, 1013, 1418, 1517, 1885
Bse1I	ACTGG	8 cut(s)	293, 451, 950, 978, 1018, 1423, 1496, 1721
Bse21I	CCTNAGG	1 cut(s)	957
BseBI	CCWGG	2 cut(s)	90, 1368
BseDI	CCNNGG	2 cut(s)	60, 1206
BseGI	GGATG	6 cut(s)	772, 865, 972, 1104, 1511, 1804
BseLI	CCNNNNNNNGG	5 cut(s)	89, 1013, 1418, 1517, 1885
BseMII	CTCAG	6 cut(s)	134, 779, 948, 1035, 1227, 1604
BseNI	ACTGG	8 cut(s)	293, 451, 950, 978, 1018, 1423, 1496, 1721
BseRI	GAGGAG	9 cut(s)	21, 72, 75, 771, 974, 1223, 1482, 1515, 1745
BseXI	GCAGC	1 cut(s)	694
BseYI	CCCAGC	2 cut(s)	261, 339
BsgI	GTGCAG	2 cut(s)	1554, 1844
Bsh1236I	CGCG	2 cut(s)	1177, 1998
BshFI	GGCC	4 cut(s)	671, 1278, 1422, 1479
BsiHKAI	GWGCWC	3 cut(s)	319, 1050, 1525
BsiSI	CCGG	3 cut(s)	241, 1808, 1886
BslFI	GGGAC	3 cut(s)	1004, 1010, 1824
BslI	CCNNNNNNNGG	5 cut(s)	89, 1013, 1418, 1517, 1885
BsmAI	GTCTC	2 cut(s)	946, 1112
BsmFI	GGGAC	3 cut(s)	1004, 1010, 1824
BsmI	GAATGC	1 cut(s)	359
BsnI	GGCC	4 cut(s)	671, 1278, 1422, 1479
Bso31I	GGTCTC	1 cut(s)	946
Bsp1286I	GDGCHC	3 cut(s)	319, 1050, 1525
Bsp143I	GATC	5 cut(s)	268, 910, 969, 1069, 1149
Bsp1720I	GCTNAGC	1 cut(s)	120
BspACI	CCGC	2 cut(s)	1177, 1998
BspANI	GGCC	4 cut(s)	671, 1278, 1422, 1479
BspCNI	CTCAG	6 cut(s)	133, 780, 949, 1036, 1228, 1603
BspFNI	CGCG	2 cut(s)	1177, 1998
BspLI	GGNNCC	8 cut(s)	10, 142, 912, 1181, 1201, 1297, 1737, 1812
BspMAI	CTGCAG	1 cut(s)	1873
BspPI	GGATC	4 cut(s)	905, 918, 964, 1064
BspQI	GCTCTTC	1 cut(s)	276
BspTNI	GGTCTC	1 cut(s)	946
BsrI	ACTGG	8 cut(s)	293, 451, 950, 978, 1018, 1423, 1496, 1721
BssECI	CCNNGG	2 cut(s)	60, 1206
BssMI	GATC	5 cut(s)	268, 910, 969, 1069, 1149
BssSI	CACGAG	2 cut(s)	125, 1431
BssT1I	CCWWGG	1 cut(s)	60
Bst2BI	CACGAG	2 cut(s)	125, 1431
Bst2UI	CCWGG	2 cut(s)	90, 1368
Bst4CI	ACNGT	7 cut(s)	394, 607, 618, 773, 1450, 1822, 1843
Bst6I	CTCTTC	6 cut(s)	276, 378, 792, 861, 944, 2003
BstAPI	GCANNNNNTGC	1 cut(s)	1651
BstBAI	YACGTR	1 cut(s)	1265
BstC8I	GCNNGC	1 cut(s)	288
BstENI	CCTNNNNNAGG	1 cut(s)	87
BstF5I	GGATG	6 cut(s)	772, 865, 972, 1104, 1511, 1804
BstFNI	CGCG	2 cut(s)	1177, 1998
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	4 cut(s)	240, 1177, 1964, 1998
BstKTI	GATC	5 cut(s)	271, 913, 972, 1072, 1152
BstMAI	GTCTC	2 cut(s)	946, 1112
BstMBI	GATC	5 cut(s)	268, 910, 969, 1069, 1149
BstMWI	GCNNNNNNNGC	4 cut(s)	35, 1232, 1651, 1756
BstNI	CCWGG	2 cut(s)	90, 1368
BstNSI	RCATGY	3 cut(s)	550, 601, 1390
BstSCI	CCNGG	4 cut(s)	88, 240, 1366, 1807
BstSFI	CTRYAG	2 cut(s)	1584, 1869
BstUI	CGCG	2 cut(s)	1177, 1998
BstV1I	GCAGC	1 cut(s)	694
BstX2I	RGATCY	2 cut(s)	910, 1149
BstYI	RGATCY	2 cut(s)	910, 1149
Bsu36I	CCTNAGG	1 cut(s)	957
BsuRI	GGCC	4 cut(s)	671, 1278, 1422, 1479
BtsCI	GGATG	6 cut(s)	772, 865, 972, 1104, 1511, 1804
BtsIMutI	CAGTG	4 cut(s)	785, 985, 1460, 1647
Cac8I	GCNNGC	1 cut(s)	288
CaiI	CAGNNNCTG	1 cut(s)	380
CfoI	GCGC	4 cut(s)	240, 1177, 1964, 1998
Cfr13I	GGNCC	5 cut(s)	140, 1179, 1296, 1370, 1811
CviAII	CATG	5 cut(s)	547, 598, 994, 1051, 1387
DpnI	GATC	5 cut(s)	270, 912, 971, 1071, 1151
DpnII	GATC	5 cut(s)	268, 910, 969, 1069, 1149
DraIII	CACNNNGTG	1 cut(s)	1265
Eam1104I	CTCTTC	6 cut(s)	276, 378, 792, 861, 944, 2003
EarI	CTCTTC	6 cut(s)	276, 378, 792, 861, 944, 2003
Ecl136II	GAGCTC	1 cut(s)	1523
Eco130I	CCWWGG	1 cut(s)	60
Eco24I	GRGCYC	1 cut(s)	1525
Eco31I	GGTCTC	1 cut(s)	946
Eco32I	GATATC	1 cut(s)	1257
Eco47I	GGWCC	5 cut(s)	140, 1179, 1296, 1370, 1811
Eco53kI	GAGCTC	1 cut(s)	1523
Eco57I	CTGAAG	4 cut(s)	402, 426, 1377, 1623
Eco81I	CCTNAGG	1 cut(s)	957
EcoICRI	GAGCTC	1 cut(s)	1523
EcoNI	CCTNNNNNAGG	1 cut(s)	87
EcoRI	GAATTC	1 cut(s)	251
EcoRII	CCWGG	2 cut(s)	88, 1366
EcoRV	GATATC	1 cut(s)	1257
EcoT14I	CCWWGG	1 cut(s)	60
EcoT22I	ATGCAT	3 cut(s)	229, 580, 1692
EcoT38I	GRGCYC	1 cut(s)	1525
ErhI	CCWWGG	1 cut(s)	60
FaeI	CATG	5 cut(s)	550, 601, 997, 1054, 1390
FalI	AAGNNNNNCTT	4 cut(s)	1776, 1808, 1866, 1898
FaqI	GGGAC	3 cut(s)	1004, 1010, 1824
FatI	CATG	5 cut(s)	546, 597, 993, 1050, 1386
FauNDI	CATATG	1 cut(s)	1647
FbaI	TGATCA	1 cut(s)	268
FblI	GTMKAC	1 cut(s)	602
Fnu4HI	GCNGC	1 cut(s)	708
FokI	GGATG	6 cut(s)	779, 872, 979, 1111, 1498, 1791
FriOI	GRGCYC	1 cut(s)	1525
Fsp4HI	GCNGC	1 cut(s)	708
FspBI	CTAG	4 cut(s)	173, 428, 611, 1122
GlaI	GCGC	4 cut(s)	239, 1176, 1963, 1997
GluI	GCNGC	1 cut(s)	708
GsaI	CCCAGC	2 cut(s)	265, 343
GsuI	CTGGAG	6 cut(s)	72, 276, 333, 653, 1479, 1704
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	4 cut(s)	671, 1278, 1422, 1479
HapII	CCGG	3 cut(s)	241, 1808, 1886
HhaI	GCGC	4 cut(s)	240, 1177, 1964, 1998
Hin1II	CATG	5 cut(s)	550, 601, 997, 1054, 1390
Hin6I	GCGC	4 cut(s)	238, 1175, 1962, 1996
HinP1I	GCGC	4 cut(s)	238, 1175, 1962, 1996
HindIII	AAGCTT	1 cut(s)	196
HinfI	GANTC	6 cut(s)	93, 169, 329, 1111, 1240, 1766
HpaII	CCGG	3 cut(s)	241, 1808, 1886
Hpy166II	GTNNAC	6 cut(s)	390, 603, 776, 1168, 1345, 1596
Hpy188I	TCNGA	6 cut(s)	250, 376, 465, 1045, 1837, 1854
Hpy188III	TCNNGA	8 cut(s)	173, 404, 914, 1115, 1155, 1187, 1682, 1781
Hpy8I	GTNNAC	6 cut(s)	390, 603, 776, 1168, 1345, 1596
HpyAV	CCTTC	7 cut(s)	401, 649, 661, 1401, 1433, 1647, 1998
HpyCH4III	ACNGT	7 cut(s)	394, 607, 618, 773, 1450, 1822, 1843
HpyCH4IV	ACGT	1 cut(s)	1264
HpyF10VI	GCNNNNNNNGC	4 cut(s)	35, 1232, 1651, 1756
HpySE526I	ACGT	1 cut(s)	1264
Hsp92II	CATG	5 cut(s)	550, 601, 997, 1054, 1390
HspAI	GCGC	4 cut(s)	238, 1175, 1962, 1996
Ksp22I	TGATCA	1 cut(s)	268
Kzo9I	GATC	5 cut(s)	268, 910, 969, 1069, 1149
LguI	GCTCTTC	1 cut(s)	276
LmnI	GCTCC	7 cut(s)	34, 295, 314, 1018, 1468, 1520, 1528
Lsp1109I	GCAGC	1 cut(s)	694
LweI	GCATC	8 cut(s)	565, 634, 801, 861, 1327, 1641, 1699, 1813
MaeI	CTAG	4 cut(s)	173, 428, 611, 1122
MaeII	ACGT	1 cut(s)	1264
MaeIII	GTNAC	2 cut(s)	696, 898
MalI	GATC	5 cut(s)	270, 912, 971, 1071, 1151
MboI	GATC	5 cut(s)	268, 910, 969, 1069, 1149
MfeI	CAATTG	1 cut(s)	711
MflI	RGATCY	2 cut(s)	910, 1149
MhlI	GDGCHC	3 cut(s)	319, 1050, 1525
MmeI	TCCRAC	2 cut(s)	1498, 1903
Mph1103I	ATGCAT	3 cut(s)	229, 580, 1692
MseI	TTAA	1 cut(s)	438
MslI	CAYNNNNRTG	1 cut(s)	726
MspI	CCGG	3 cut(s)	241, 1808, 1886
MspR9I	CCNGG	4 cut(s)	90, 242, 1368, 1809
MunI	CAATTG	1 cut(s)	711
Mva1269I	GAATGC	1 cut(s)	359
MvaI	CCWGG	2 cut(s)	90, 1368
MvnI	CGCG	2 cut(s)	1177, 1998
MwoI	GCNNNNNNNGC	4 cut(s)	35, 1232, 1651, 1756
NciI	CCSGG	2 cut(s)	242, 1809
NdeI	CATATG	1 cut(s)	1647
NdeII	GATC	5 cut(s)	268, 910, 969, 1069, 1149
NlaIII	CATG	5 cut(s)	550, 601, 997, 1054, 1390
NlaIV	GGNNCC	8 cut(s)	10, 142, 912, 1181, 1201, 1297, 1737, 1812
NsiI	ATGCAT	3 cut(s)	229, 580, 1692
NspI	RCATGY	3 cut(s)	550, 601, 1390
PciI	ACATGT	1 cut(s)	597
PciSI	GCTCTTC	1 cut(s)	276
PctI	GAATGC	1 cut(s)	359
PfeI	GAWTC	6 cut(s)	93, 169, 329, 1111, 1240, 1766
PflMI	CCANNNNNTGG	2 cut(s)	1418, 1517
PfoI	TCCNGGA	2 cut(s)	88, 1807
PkrI	GCNGC	1 cut(s)	709
Ppu21I	YACGTR	1 cut(s)	1265
PscI	ACATGT	1 cut(s)	597
PsiI	TTATAA	2 cut(s)	234, 806
Psp124BI	GAGCTC	1 cut(s)	1525
Psp6I	CCWGG	2 cut(s)	88, 1366
PspFI	CCCAGC	2 cut(s)	261, 339
PspGI	CCWGG	2 cut(s)	88, 1366
PspN4I	GGNNCC	8 cut(s)	10, 142, 912, 1181, 1201, 1297, 1737, 1812
PspPI	GGNCC	5 cut(s)	140, 1179, 1296, 1370, 1811
PstI	CTGCAG	1 cut(s)	1873
PstNI	CAGNNNCTG	1 cut(s)	380
PsuI	RGATCY	2 cut(s)	910, 1149
RseI	CAYNNNNRTG	1 cut(s)	726
SacI	GAGCTC	1 cut(s)	1525
SapI	GCTCTTC	1 cut(s)	276
SaqAI	TTAA	1 cut(s)	438
SatI	GCNGC	1 cut(s)	708
Sau3AI	GATC	5 cut(s)	268, 910, 969, 1069, 1149
Sau96I	GGNCC	5 cut(s)	140, 1179, 1296, 1370, 1811
ScrFI	CCNGG	4 cut(s)	90, 242, 1368, 1809
SduI	GDGCHC	3 cut(s)	319, 1050, 1525
SfaNI	GCATC	8 cut(s)	565, 634, 801, 861, 1327, 1641, 1699, 1813
SfcI	CTRYAG	2 cut(s)	1584, 1869
SinI	GGWCC	5 cut(s)	140, 1179, 1296, 1370, 1811
SmiMI	CAYNNNNRTG	1 cut(s)	726
SmlI	CTYRAG	4 cut(s)	530, 1153, 1680, 1925
SmoI	CTYRAG	4 cut(s)	530, 1153, 1680, 1925
SpeI	ACTAGT	1 cut(s)	1121
SsiI	CCGC	2 cut(s)	1177, 1998
SspI	AATATT	2 cut(s)	1545, 1564
SspMI	CTAG	4 cut(s)	173, 428, 611, 1122
SstI	GAGCTC	1 cut(s)	1525
StyD4I	CCNGG	4 cut(s)	88, 240, 1366, 1807
StyI	CCWWGG	1 cut(s)	60
TaaI	ACNGT	7 cut(s)	394, 607, 618, 773, 1450, 1822, 1843
TaiI	ACGT	1 cut(s)	1267
TaqI	TCGA	4 cut(s)	795, 846, 1399, 1761
TfiI	GAWTC	6 cut(s)	93, 169, 329, 1111, 1240, 1766
Tru1I	TTAA	1 cut(s)	438
Tru9I	TTAA	1 cut(s)	438
TscAI	CASTG	4 cut(s)	792, 985, 1460, 1647
TseI	GCWGC	1 cut(s)	707
TspDTI	ATGAA	3 cut(s)	294, 876, 1067
TspGWI	ACGGA	2 cut(s)	153, 1504
TspRI	CASTG	4 cut(s)	792, 985, 1460, 1647
Van91I	CCANNNNNTGG	2 cut(s)	1418, 1517
VpaK11BI	GGWCC	5 cut(s)	140, 1179, 1296, 1370, 1811
XagI	CCTNNNNNAGG	1 cut(s)	87
XapI	RAATTY	3 cut(s)	251, 411, 1863
XbaI	TCTAGA	1 cut(s)	172
XceI	RCATGY	3 cut(s)	550, 601, 1390
XmiI	GTMKAC	1 cut(s)	602
XspI	CTAG	4 cut(s)	173, 428, 611, 1122
Zsp2I	ATGCAT	3 cut(s)	229, 580, 1692

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.