Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0001911

Physical Location & Seq

Genomic Coordinates Reverse (-)

1865558 .. 1869289

Gene Structure 3732 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G002110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGCTCAAGAGAAGGAAATGAGCAGGGTGACTGAGTGGAGAATAAGAATGGCGGATGGTTCATCTGAAGCTTTGGCTCCAGAAGCAGGGCTTGCCTGCCGAGCTTGGCTTGCTCTGAAGGGTCTGATTATGGGGTTGGTTTTGAAAGTGTGCAATTTCTTCAGGAAAGCTTGGGATTTGGGACGAAATGAGCCTAAAAAAGTGATTCATTGTTTGAAAGTAGGGATGGCACTCACTGTTGTTTCACTGTTTTACTACATGAGACCTTTGTACGTAGGTATGGGAGGAAATGCTATGTGGGCAGTTATCACTGTTGTGGTTGTCTTTGAAAACACAGTGGGTGCAACAATATGTAAAAGTTTAAACAGAATCTGTGGAACTTTTGTGGCTGGCTCTCTTGCCATTGGCATCCATTGGATAGTGAGTCAATCAGGAAAAGAATTCGAGCCTTTCATCAATGGGATTTCAGTATTTTTGTTAGCTTCTGCAGTTACCTTCTCAAGATTTATACCATCAGTGAAAAAGCGATTTGATTATGGTGCAATGATCTTCATCCTCACATTCAGTTTAGTTACAGTGTCGGGTTACCGGGTGGATAAATTATTGGAAATGGCCAACGATAGAGTATACACCATCATCATTGGAACTTCATTGTGCATTATGATAACCATGATTATTTGCCCCATTTGGGCTGGGGAGGGACTCTATATGCTCATCACTCGAAACATGGACAAACTTGCCTATTCGTTAGATGGTTGTGTTGGAGAGTATTTAAATGATAGCGGAAGCTCTTCTGATGGTGACAAGGAATCTGATAAGAAATTGATTGGCTACAAATGTGTTTTAACCTCAAAAGCAACAGAAGAAACCATGGCTAATTTTGCAAGATGGGAGCCTGCACATGGCCGCTTCAACTCCAGACATCTATGGAAACAATACCTCAAAATCGGCGCATCAATGCGTGCTTGTGCTTATTGCATTGACACCCTGGATGGTTGTGTGAATTCCGAAAATGAGGTTCCTGAGTTGATAAAGAAGCATATGAGCAACATTACTTTGAAAGTAAGCTCTGAGTCTTCAAGAGTCATAAAAGAGCTTGCAAGGACTATGAAGACAATGAAGAAATCATCTACTATAGATTATTTAGTTGGAGAAATGAACAATGCAGTCTTGAAGCTCCAAGAGGACTTAAAATCTCTTCCTACTTTATTTATTAATCCGCAGCCACTGCAAGGAGCTGACTGTCCTGAAAAAAGGAACCCAGGAGCAGTTGCTCTCATGGATATCATGCCACTTGTGACTTTAGCTTCCTTACTGATTGGGATTGCTACAAGGATTGAAGGCATGGTTAATGCCGTTGAGGAACTAGCTGAGATGGGTGAGTACAAGTCTGTTGCAGATGAGAAGAATCAAAACCAGCCTGTTAGTAAAAGTGTAAAACTAATGTACCTGCAGATGAGCATAAGGAAGTGGTGTGAGGGTTGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0008150 GO:0008509 GO:0008514 GO:0009705 GO:0015075 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071423 GO:0071702 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALMT FUSC_2

Protein Analysis

496

Amino Acids

55.03

Weight (kDa)

8.67

Isoelectric Point (pI)

33.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALMT	PF11744	58 - 409	4e-142	Aluminium activated malate transporter
FUSC_2	PF13515	90 - 221	6.6e-10	Fusaric acid resistance protein-like
FUSC	PF04632	97 - 309	3.9e-08	Fusaric acid resistance protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00910
fragaria_vesca	FvH4_4g16071 FvH4_5g11291
malus_domestica	MD06G1114500.v1.1 MD07G1153600.v1.1 MD14G1135700.v1.1 MD14G1135900.v1.1
prunus_persica	Prupe.2G192500_v2.0.a1 Prupe.5G127200_v2.0.a1
pyrus_communis	pycom07g15140
rosa_chinensis	RchiOBHm_Chr1g0360401 RchiOBHm_Chr3g0450301 RchiOBHm_Chr7g0186561
rosa_laevigata	RLG00000004835 RLG00000027810
rosa_multiflora	Rmu_co8404289.1_g000001 Rmu_sc0000645.1_g000020 Rmu_sc0005298.1_g000005 Rmu_sc0007722.1_g000021 Rmu_ssc0000368.1_g000080
rosa_roxburghii	Rroxscaffold_3G00268050 Rroxscaffold_4G00296140 Rroxscaffold_6G00428020
rosa_rugosa	Rorug01G0276700 Rorug02G0626700 Rorug06G0475100
rosa_samantha	Rh1AG290700 Rh1BG255800 Rh1CG273500 Rh1DG285600 Rh3AG026300 Rh3AG026900 Rh3BG027000 Rh3BG027500 Rh3CG026000 Rh3CG026400 Rh3DG026900 Rh3DG027400 Rh7AG066000 Rh7BG081900 Rh7CG081800 Rh7DG082800
rosa_wichuraiana	Rw1G025740 Rw3G002110 Rw7G006880 Rw7G007100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1458
AccI	GTMKAC	1 cut(s)	627
AciI	CCGC	4 cut(s)	53, 783, 907, 1220
AcoI	YGGCCR	2 cut(s)	612, 904
AcsI	RAATTY	2 cut(s)	440, 1003
AcuI	CTGAAG	3 cut(s)	87, 137, 145
AfaI	GTAC	3 cut(s)	272, 1385, 1448
AfiI	CCNNNNNNNGG	4 cut(s)	86, 688, 902, 1232
AgsI	TTSAA	8 cut(s)	145, 217, 329, 913, 1060, 1080, 1174, 1340
AjnI	CCWGG	2 cut(s)	987, 1261
AleI	CACNNNNGTG	1 cut(s)	314
Alw26I	GTCTC	1 cut(s)	256
AlwNI	CAGNNNCTG	2 cut(s)	372, 1228
AoxI	GGCC	2 cut(s)	612, 904
ApeKI	GCWGC	1 cut(s)	1222
ApoI	RAATTY	2 cut(s)	440, 1003
AseI	ATTAAT	1 cut(s)	1215
Asp700I	GAANNNNTTC	1 cut(s)	790
AspLEI	GCGC	1 cut(s)	953
AsuC2I	CCSGG	1 cut(s)	590
AsuHPI	GGTGA	3 cut(s)	40, 812, 1391
BaeI	ACNNNNGTAYC	2 cut(s)	1430, 1463
BalI	TGGCCA	1 cut(s)	614
BbsI	GAAGAC	2 cut(s)	1068, 1118
BbvI	GCAGC	1 cut(s)	1234
BccI	CCATC	9 cut(s)	50, 220, 520, 641, 746, 791, 882, 986, 1369
BceAI	ACGGC	1 cut(s)	1340
BciT130I	CCWGG	2 cut(s)	989, 1263
BcnI	CCSGG	1 cut(s)	590
BcoDI	GTCTC	1 cut(s)	256
BfaI	CTAG	1 cut(s)	1367
BfmI	CTRYAG	3 cut(s)	486, 1134, 1451
BfuAI	ACCTGC	1 cut(s)	1458
BisI	GCNGC	2 cut(s)	907, 1223
BlsI	GCNGC	2 cut(s)	908, 1224
Bme1390I	CCNGG	3 cut(s)	590, 989, 1263
BmiI	GGNNCC	4 cut(s)	78, 894, 1020, 1259
BmrFI	CCNGG	3 cut(s)	590, 989, 1263
BmsI	GCATC	2 cut(s)	417, 962
BpiI	GAAGAC	2 cut(s)	1068, 1118
BpmI	CTGGAG	2 cut(s)	63, 901
BpuEI	CTTGAG	1 cut(s)	484
BpuMI	CCSGG	1 cut(s)	590
BsaAI	YACGTR	1 cut(s)	274
BsaBI	GATNNNNATC	1 cut(s)	551
BsaI	GGTCTC	1 cut(s)	256
BsaJI	CCNNGG	3 cut(s)	870, 987, 1261
Bsc4I	CCNNNNNNNGG	4 cut(s)	86, 688, 902, 1232
Bse3DI	GCAATG	1 cut(s)	549
Bse8I	GATNNNNATC	1 cut(s)	551
BseBI	CCWGG	2 cut(s)	989, 1263
BseDI	CCNNGG	3 cut(s)	870, 987, 1261
BseGI	GGATG	5 cut(s)	61, 231, 408, 552, 997
BseJI	GATNNNNATC	1 cut(s)	551
BseLI	CCNNNNNNNGG	4 cut(s)	86, 688, 902, 1232
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	4 cut(s)	24, 1014, 1062, 1362
BseXI	GCAGC	1 cut(s)	1234
BseYI	CCCAGC	1 cut(s)	692
BsgI	GTGCAG	1 cut(s)	882
BshFI	GGCC	2 cut(s)	614, 906
BsiSI	CCGG	1 cut(s)	589
BslFI	GGGAC	2 cut(s)	195, 714
BslI	CCNNNNNNNGG	4 cut(s)	86, 688, 902, 1232
BsmAI	GTCTC	1 cut(s)	256
BsmFI	GGGAC	2 cut(s)	195, 714
BsnI	GGCC	2 cut(s)	614, 906
Bso31I	GGTCTC	1 cut(s)	256
Bsp143I	GATC	1 cut(s)	546
Bsp19I	CCATGG	1 cut(s)	870
BspACI	CCGC	4 cut(s)	53, 783, 907, 1220
BspANI	GGCC	2 cut(s)	614, 906
BspCNI	CTCAG	4 cut(s)	25, 1015, 1063, 1363
BspLI	GGNNCC	4 cut(s)	78, 894, 1020, 1259
BspMAI	CTGCAG	2 cut(s)	490, 1455
BspMI	ACCTGC	1 cut(s)	1458
BspQI	GCTCTTC	1 cut(s)	796
BspTNI	GGTCTC	1 cut(s)	256
BsrDI	GCAATG	1 cut(s)	549
BssECI	CCNNGG	3 cut(s)	870, 987, 1261
BssMI	GATC	1 cut(s)	546
BssNAI	GTATAC	1 cut(s)	628
BssT1I	CCWWGG	1 cut(s)	870
Bst1107I	GTATAC	1 cut(s)	628
Bst2UI	CCWGG	2 cut(s)	989, 1263
Bst4CI	ACNGT	6 cut(s)	238, 249, 313, 337, 577, 1244
Bst6I	CTCTTC	2 cut(s)	796, 1203
BstAPI	GCANNNNNTGC	2 cut(s)	92, 1228
BstBAI	YACGTR	1 cut(s)	274
BstC8I	GCNNGC	7 cut(s)	93, 97, 111, 391, 897, 963, 1098
BstDEI	CTNAG	4 cut(s)	33, 1023, 1071, 1371
BstDSI	CCRYGG	1 cut(s)	870
BstEII	GGTNACC	1 cut(s)	584
BstF5I	GGATG	5 cut(s)	61, 231, 408, 552, 997
BstHHI	GCGC	1 cut(s)	953
BstKTI	GATC	1 cut(s)	549
BstMAI	GTCTC	1 cut(s)	256
BstMBI	GATC	1 cut(s)	546
BstMWI	GCNNNNNNNGC	7 cut(s)	83, 92, 101, 110, 299, 881, 1228
BstNI	CCWGG	2 cut(s)	989, 1263
BstPI	GGTNACC	1 cut(s)	584
BstSCI	CCNGG	3 cut(s)	588, 987, 1261
BstSFI	CTRYAG	3 cut(s)	486, 1134, 1451
BstSNI	TACGTA	1 cut(s)	274
BstV1I	GCAGC	1 cut(s)	1234
BstV2I	GAAGAC	2 cut(s)	1068, 1118
BstZ17I	GTATAC	1 cut(s)	628
BsuRI	GGCC	2 cut(s)	614, 906
BtgI	CCRYGG	1 cut(s)	870
BtsCI	GGATG	5 cut(s)	61, 231, 408, 552, 997
BtsI	GCAGTG	1 cut(s)	1226
BtsIMutI	CAGTG	7 cut(s)	234, 245, 309, 342, 522, 582, 1226
BveI	ACCTGC	1 cut(s)	1458
Cac8I	GCNNGC	7 cut(s)	93, 97, 111, 391, 897, 963, 1098
CaiI	CAGNNNCTG	2 cut(s)	372, 1228
CfoI	GCGC	1 cut(s)	953
Csp6I	GTAC	3 cut(s)	271, 1384, 1447
CviAII	CATG	8 cut(s)	259, 670, 727, 871, 902, 1279, 1288, 1345
CviQI	GTAC	3 cut(s)	271, 1384, 1447
DdeI	CTNAG	4 cut(s)	33, 1023, 1071, 1371
DpnI	GATC	1 cut(s)	548
DpnII	GATC	1 cut(s)	546
DraI	TTTAAA	2 cut(s)	363, 774
EaeI	YGGCCR	2 cut(s)	612, 904
Eam1104I	CTCTTC	2 cut(s)	796, 1203
EarI	CTCTTC	2 cut(s)	796, 1203
EciI	GGCGGA	1 cut(s)	68
Eco105I	TACGTA	1 cut(s)	274
Eco130I	CCWWGG	1 cut(s)	870
Eco31I	GGTCTC	1 cut(s)	256
Eco32I	GATATC	1 cut(s)	1285
Eco57I	CTGAAG	3 cut(s)	87, 137, 145
Eco91I	GGTNACC	1 cut(s)	584
EcoO65I	GGTNACC	1 cut(s)	584
EcoRI	GAATTC	2 cut(s)	440, 1003
EcoRII	CCWGG	2 cut(s)	987, 1261
EcoRV	GATATC	1 cut(s)	1285
EcoT14I	CCWWGG	1 cut(s)	870
ErhI	CCWWGG	1 cut(s)	870
FaeI	CATG	8 cut(s)	262, 673, 730, 874, 905, 1282, 1291, 1348
FalI	AAGNNNNNCTT	2 cut(s)	75, 107
FaqI	GGGAC	2 cut(s)	195, 714
FatI	CATG	8 cut(s)	258, 669, 726, 870, 901, 1278, 1287, 1344
FauNDI	CATATG	1 cut(s)	1041
FblI	GTMKAC	1 cut(s)	627
Fnu4HI	GCNGC	2 cut(s)	907, 1223
FokI	GGATG	5 cut(s)	68, 238, 395, 539, 1004
Fsp4HI	GCNGC	2 cut(s)	907, 1223
FspBI	CTAG	1 cut(s)	1367
GlaI	GCGC	1 cut(s)	952
GluI	GCNGC	2 cut(s)	907, 1223
GsaI	CCCAGC	1 cut(s)	696
GsuI	CTGGAG	2 cut(s)	63, 901
HaeIII	GGCC	2 cut(s)	614, 906
HapII	CCGG	1 cut(s)	589
HhaI	GCGC	1 cut(s)	953
Hin1II	CATG	8 cut(s)	262, 673, 730, 874, 905, 1282, 1291, 1348
Hin6I	GCGC	1 cut(s)	951
HinP1I	GCGC	1 cut(s)	951
HindIII	AAGCTT	2 cut(s)	69, 168
HinfI	GANTC	8 cut(s)	205, 369, 424, 702, 809, 1073, 1083, 1408
HpaII	CCGG	1 cut(s)	589
HphI	GGTGA	3 cut(s)	40, 812, 1391
Hpy166II	GTNNAC	1 cut(s)	628
Hpy188I	TCNGA	7 cut(s)	67, 117, 126, 796, 814, 1009, 1072
Hpy8I	GTNNAC	1 cut(s)	628
HpyAV	CCTTC	4 cut(s)	7, 112, 505, 1334
HpyCH4III	ACNGT	6 cut(s)	238, 249, 313, 337, 577, 1244
HpyCH4IV	ACGT	1 cut(s)	273
HpyF10VI	GCNNNNNNNGC	7 cut(s)	83, 92, 101, 110, 299, 881, 1228
HpyF3I	CTNAG	4 cut(s)	33, 1023, 1071, 1371
HpySE526I	ACGT	1 cut(s)	273
Hsp92II	CATG	8 cut(s)	262, 673, 730, 874, 905, 1282, 1291, 1348
HspAI	GCGC	1 cut(s)	951
Kzo9I	GATC	1 cut(s)	546
LguI	GCTCTTC	1 cut(s)	796
LmnI	GCTCC	5 cut(s)	82, 892, 1182, 1235, 1265
Lsp1109I	GCAGC	1 cut(s)	1234
LweI	GCATC	2 cut(s)	417, 962
MaeI	CTAG	1 cut(s)	1367
MaeII	ACGT	1 cut(s)	273
MaeIII	GTNAC	6 cut(s)	28, 490, 571, 584, 800, 1297
MalI	GATC	1 cut(s)	548
MboI	GATC	1 cut(s)	546
MboII	GAAGA	9 cut(s)	151, 541, 783, 875, 1068, 1123, 1132, 1190, 1417
MlsI	TGGCCA	1 cut(s)	614
MluCI	AATT	6 cut(s)	154, 440, 599, 821, 877, 1003
MluNI	TGGCCA	1 cut(s)	614
MlyI	GAGTC	4 cut(s)	433, 696, 1082, 1092
MmeI	TCCRAC	2 cut(s)	742, 1129
MnlI	CCTC	9 cut(s)	278, 566, 691, 859, 950, 1009, 1177, 1354, 1471
Mox20I	TGGCCA	1 cut(s)	614
MroXI	GAANNNNTTC	1 cut(s)	790
MscI	TGGCCA	1 cut(s)	614
MseI	TTAA	6 cut(s)	362, 773, 845, 1190, 1215, 1350
MslI	CAYNNNNRTG	1 cut(s)	314
Msp20I	TGGCCA	1 cut(s)	614
MspI	CCGG	1 cut(s)	589
MspR9I	CCNGG	3 cut(s)	590, 989, 1263
MssI	GTTTAAAC	1 cut(s)	363
MvaI	CCWGG	2 cut(s)	989, 1263
MwoI	GCNNNNNNNGC	7 cut(s)	83, 92, 101, 110, 299, 881, 1228
NciI	CCSGG	1 cut(s)	590
NcoI	CCATGG	1 cut(s)	870
NdeI	CATATG	1 cut(s)	1041
NdeII	GATC	1 cut(s)	546
NlaIII	CATG	8 cut(s)	262, 673, 730, 874, 905, 1282, 1291, 1348
NlaIV	GGNNCC	4 cut(s)	78, 894, 1020, 1259
NmeAIII	GCCGAG	1 cut(s)	125
NmuCI	GTSAC	3 cut(s)	28, 800, 1297
OliI	CACNNNNGTG	1 cut(s)	314
PciSI	GCTCTTC	1 cut(s)	796
PdmI	GAANNNNTTC	1 cut(s)	790
PfeI	GAWTC	4 cut(s)	205, 369, 809, 1408
PkrI	GCNGC	2 cut(s)	908, 1224
PleI	GAGTC	4 cut(s)	432, 696, 1081, 1091
PmeI	GTTTAAAC	1 cut(s)	363
PpsI	GAGTC	4 cut(s)	432, 696, 1081, 1091
Ppu21I	YACGTR	1 cut(s)	274
PshBI	ATTAAT	1 cut(s)	1215
Psp6I	CCWGG	2 cut(s)	987, 1261
PspEI	GGTNACC	1 cut(s)	584
PspFI	CCCAGC	1 cut(s)	692
PspGI	CCWGG	2 cut(s)	987, 1261
PspN4I	GGNNCC	4 cut(s)	78, 894, 1020, 1259
PstI	CTGCAG	2 cut(s)	490, 1455
PstNI	CAGNNNCTG	2 cut(s)	372, 1228
RsaI	GTAC	3 cut(s)	272, 1385, 1448
RsaNI	GTAC	3 cut(s)	271, 1384, 1447
RseI	CAYNNNNRTG	1 cut(s)	314
SapI	GCTCTTC	1 cut(s)	796
SaqAI	TTAA	6 cut(s)	362, 773, 845, 1190, 1215, 1350
SatI	GCNGC	2 cut(s)	907, 1223
Sau3AI	GATC	1 cut(s)	546
SchI	GAGTC	4 cut(s)	433, 696, 1082, 1092
ScrFI	CCNGG	3 cut(s)	590, 989, 1263
SfaNI	GCATC	2 cut(s)	417, 962
SfcI	CTRYAG	3 cut(s)	486, 1134, 1451
SmiI	ATTTAAAT	1 cut(s)	774
SmiMI	CAYNNNNRTG	1 cut(s)	314
SmlI	CTYRAG	2 cut(s)	6, 499
SmoI	CTYRAG	2 cut(s)	6, 499
SnaBI	TACGTA	1 cut(s)	274
Sse9I	AATT	6 cut(s)	154, 440, 599, 821, 877, 1003
SsiI	CCGC	4 cut(s)	53, 783, 907, 1220
SspMI	CTAG	1 cut(s)	1367
StyD4I	CCNGG	3 cut(s)	588, 987, 1261
StyI	CCWWGG	1 cut(s)	870
SwaI	ATTTAAAT	1 cut(s)	774
TaaI	ACNGT	6 cut(s)	238, 249, 313, 337, 577, 1244
TaiI	ACGT	1 cut(s)	276
TaqI	TCGA	2 cut(s)	444, 721
TasI	AATT	6 cut(s)	154, 440, 599, 821, 877, 1003
TatI	WGTACW	1 cut(s)	1383
TauI	GCSGC	1 cut(s)	909
TfiI	GAWTC	4 cut(s)	205, 369, 809, 1408
Tru1I	TTAA	6 cut(s)	362, 773, 845, 1190, 1215, 1350
Tru9I	TTAA	6 cut(s)	362, 773, 845, 1190, 1215, 1350
TscAI	CASTG	7 cut(s)	241, 252, 316, 342, 522, 582, 1233
TseFI	GTSAC	3 cut(s)	28, 800, 1297
TseI	GCWGC	1 cut(s)	1222
Tsp45I	GTSAC	3 cut(s)	28, 800, 1297
TspDTI	ATGAA	8 cut(s)	51, 197, 442, 541, 639, 1124, 1133, 1172
TspRI	CASTG	7 cut(s)	241, 252, 316, 342, 522, 582, 1233
VspI	ATTAAT	1 cut(s)	1215
XapI	RAATTY	2 cut(s)	440, 1003
XmiI	GTMKAC	1 cut(s)	627
XmnI	GAANNNNTTC	1 cut(s)	790
XspI	CTAG	1 cut(s)	1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.