Rw3G019660

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002527
Physical Location & Seq
Forward (+)
22727802 .. 22729896
2095 bp
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UTR
Exon/CDS
Intron
Rw3G019660.1

Sequence Viewer

Length: 483 bp
ATGGCTACAGCGACCTACCAACCTCCACCACCATATTATAAGCTATACAAAGATTACTTGCAAGATCCCAAATCCGCTCCTGAGCTGCTGCCACCAATTGAAGGGACATACAGTTGCTATGGTGCTACTTACACTACAGATAACGTGCTTCCAAGCTTGGAAGAACAAGGAGTGCATTTTAAGAAGGAACTAAAGTCCCTTAACAGAGAATTGCAGCTGCACATTTTGGAACTTGCTGATATTCTCGTGGACAGACCATCACAAAATGCAAGGAGAGTGGAAGAAATATTTCTTATATTCAAGAACTTGCATCATCTTATGAACTCATTGCGCCCTCATCAGGCTTGGGTAACACTAATTCACATTCTAGAACTTCAGATAAAACGACGTAAACAAGCTGTTGAGGATACAAAGAGATATGCACTTCTGAAGGAGTCCCTTCAATCGTTTGAGGAAACTGGTGCATCTCTTATGTTGAAATAG

Protein Analysis

160

Amino Acids

18.62

Weight (kDa)

7.02

Isoelectric Point (pI)

68.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med7 PF05983 4 - 139 1.8e-27 MED7 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
AccBSI CCGCTC 1 cut(s) 77
AciI CCGC 1 cut(s) 75
AclWI GGATC 1 cut(s) 59
AcuI CTGAAG 2 cut(s) 359, 449
AfiI CCNNNNNNNGG 2 cut(s) 101, 340
AgsI TTSAA 4 cut(s) 101, 301, 443, 478
AluBI AGCT 5 cut(s) 43, 85, 156, 217, 398
AluI AGCT 5 cut(s) 43, 85, 156, 217, 398
AlwI GGATC 1 cut(s) 59
ApeKI GCWGC 4 cut(s) 85, 88, 214, 217
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 333
BauI CACGAG 1 cut(s) 245
BbvI GCAGC 4 cut(s) 72, 75, 204, 226
BccI CCATC 1 cut(s) 265
BciVI GTATCC 1 cut(s) 400
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 2 cut(s) 6, 135
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 4 cut(s) 86, 89, 215, 218
BlsI GCNGC 4 cut(s) 87, 90, 216, 219
BmsI GCATC 2 cut(s) 319, 473
Bpu10I CCTNAGC 1 cut(s) 81
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 340
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 101, 340
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 463
BseXI GCAGC 4 cut(s) 72, 75, 204, 226
BsgI GTGCAG 1 cut(s) 203
BslFI GGGAC 3 cut(s) 118, 181, 421
BslI CCNNNNNNNGG 2 cut(s) 101, 340
BsmFI GGGAC 3 cut(s) 118, 181, 421
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 73
BspPI GGATC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 326
BsrI ACTGG 1 cut(s) 463
BssMI GATC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 113
BstDEI CTNAG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 1 cut(s) 67
BstMBI GATC 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 6, 135
BstV1I GCAGC 4 cut(s) 72, 75, 204, 226
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuI GTATCC 1 cut(s) 400
CfoI GCGC 1 cut(s) 333
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviJI RGCY 7 cut(s) 5, 43, 85, 156, 217, 344, 398
CviKI_1 RGCY 7 cut(s) 5, 43, 85, 156, 217, 344, 398
DdeI CTNAG 1 cut(s) 81
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 359, 449
FaiI YATR 9 cut(s) 34, 39, 46, 109, 120, 296, 320, 420, 473
FaqI GGGAC 3 cut(s) 118, 181, 421
Fnu4HI GCNGC 4 cut(s) 86, 89, 215, 218
Fsp4HI GCNGC 4 cut(s) 86, 89, 215, 218
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 332
GluI GCNGC 4 cut(s) 86, 89, 215, 218
HhaI GCGC 1 cut(s) 333
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 250, 392
Hpy188I TCNGA 2 cut(s) 378, 429
Hpy188III TCNNGA 3 cut(s) 80, 301, 368
Hpy8I GTNNAC 2 cut(s) 250, 392
Hpy99I CGWCG 1 cut(s) 390
HpyAV CCTTC 4 cut(s) 95, 178, 424, 449
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4IV ACGT 2 cut(s) 144, 388
HpyCH4V TGCA 8 cut(s) 61, 175, 214, 220, 269, 310, 422, 464
HpyF3I CTNAG 1 cut(s) 81
HpySE526I ACGT 2 cut(s) 144, 388
HspAI GCGC 1 cut(s) 331
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 3 cut(s) 93, 326, 444
Lsp1109I GCAGC 4 cut(s) 72, 75, 204, 226
LweI GCATC 2 cut(s) 319, 473
MaeI CTAG 1 cut(s) 368
MaeII ACGT 2 cut(s) 144, 388
MaeIII GTNAC 1 cut(s) 349
MalI GATC 1 cut(s) 66
MbiI CCGCTC 1 cut(s) 77
MboI GATC 1 cut(s) 64
MboII GAAGA 2 cut(s) 173, 293
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 64
MluCI AATT 3 cut(s) 96, 209, 357
MlyI GAGTC 1 cut(s) 443
MnlI CCTC 4 cut(s) 33, 345, 397, 445
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 2 cut(s) 180, 201
MspA1I CMGCKG 1 cut(s) 217
MunI CAATTG 1 cut(s) 96
NdeII GATC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 288
PkrI GCNGC 4 cut(s) 87, 90, 216, 219
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PsiI TTATAA 1 cut(s) 39
PsuI RGATCY 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 217
SaqAI TTAA 2 cut(s) 180, 201
SatI GCNGC 4 cut(s) 86, 89, 215, 218
Sau3AI GATC 1 cut(s) 64
SchI GAGTC 1 cut(s) 443
SetI ASST 9 cut(s) 17, 25, 45, 87, 147, 158, 219, 391, 400
SfaNI GCATC 2 cut(s) 319, 473
SfcI CTRYAG 2 cut(s) 6, 135
Sse9I AATT 3 cut(s) 96, 209, 357
SsiI CCGC 1 cut(s) 75
SspI AATATT 1 cut(s) 288
SspMI CTAG 1 cut(s) 368
TaaI ACNGT 1 cut(s) 113
TaiI ACGT 2 cut(s) 147, 391
TasI AATT 3 cut(s) 96, 209, 357
Tru1I TTAA 2 cut(s) 180, 201
Tru9I TTAA 2 cut(s) 180, 201
TseI GCWGC 4 cut(s) 85, 88, 214, 217
TspDTI ATGAA 1 cut(s) 335
XbaI TCTAGA 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.