Rw4G011080

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
Physical Location & Seq
Forward (+)
26287244 .. 26289681
2438 bp
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UTR
Exon/CDS
Intron
Rw4G011080.1

Sequence Viewer

Length: 528 bp
ATGGCTACAGCGACCTACCCACCTCCACCACCGTATTATAAGCTATACAAAGATTACTTGCAAGATCCCAAATCCGCTCCTGAGCCGCCGCCACCAATTGAAGGGACATACATTTGCTATGGTGCTAATTACACCACAGATGACGTGCTTCCAAGCTTGGAAGAACAGGGAGTGCGTCAACTGTACCCAAAAGGCCCAAATATTGATTTTAAGAAGGAACTGAGGTCCCTTAACAGAGAATTGCAGCTGCACATTTTGGAACTCGCTGATATTCTCGTGGAGAGACCATCACAATATGCAAGGAGAGTGGAAGAAATATCTCTTATATTCAAGAACTTGCATCATCTTCTGAACTCATTGCGGCCCCATCAGGCTAGGGCAACACTAATTCACATTCTAGAACTTCAGATTAAACGACGTAAACAAGCTGTTGAGGATATAAAGAGGAGGAGAGAAGAAGCACAGGCACTTCTGAAGGAGTCCCTTGAATCGTTGGAGGACACTGGTGCATCTCTTATGTTGAAATAG

Protein Analysis

175

Amino Acids

20.27

Weight (kDa)

6.98

Isoelectric Point (pI)

78.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med7 PF05983 4 - 158 1.5e-45 MED7 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
AccBSI CCGCTC 1 cut(s) 77
AciI CCGC 4 cut(s) 75, 86, 89, 361
AclWI GGATC 1 cut(s) 59
AcuI CTGAAG 2 cut(s) 389, 494
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 4 cut(s) 101, 331, 488, 523
AjiI CACGTC 1 cut(s) 145
AluBI AGCT 4 cut(s) 43, 156, 247, 428
AluI AGCT 4 cut(s) 43, 156, 247, 428
Alw26I GTCTC 1 cut(s) 277
AlwI GGATC 1 cut(s) 59
AoxI GGCC 2 cut(s) 193, 362
ApeKI GCWGC 2 cut(s) 244, 247
AspS9I GGNCC 3 cut(s) 194, 225, 363
AvaII GGWCC 1 cut(s) 225
BauI CACGAG 1 cut(s) 275
BbvI GCAGC 2 cut(s) 234, 256
BccI CCATC 2 cut(s) 295, 375
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 2 cut(s) 375, 398
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 5 cut(s) 86, 89, 245, 248, 362
BlsI GCNGC 5 cut(s) 87, 90, 246, 249, 363
Bme18I GGWCC 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 3 cut(s) 194, 225, 363
BmiI GGNNCC 2 cut(s) 227, 365
BmsI GCATC 2 cut(s) 349, 518
Bpu10I CCTNAGC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 356
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 72, 212
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 2 cut(s) 460, 463
BseXI GCAGC 2 cut(s) 234, 256
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 2 cut(s) 195, 364
BslFI GGGAC 3 cut(s) 118, 211, 466
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 277
BsmFI GGGAC 3 cut(s) 118, 211, 466
BsnI GGCC 2 cut(s) 195, 364
Bso31I GGTCTC 1 cut(s) 277
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 4 cut(s) 75, 86, 89, 361
BspANI GGCC 2 cut(s) 195, 364
BspCNI CTCAG 2 cut(s) 73, 213
BspLI GGNNCC 2 cut(s) 227, 365
BspPI GGATC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 356
BsrI ACTGG 1 cut(s) 508
BssMI GATC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 33, 183
BstDEI CTNAG 2 cut(s) 81, 221
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 234, 256
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 2 cut(s) 195, 364
BtrI CACGTC 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 501
Cfr13I GGNCC 3 cut(s) 194, 225, 363
CseI GACGC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviJI RGCY 9 cut(s) 5, 43, 85, 156, 195, 247, 364, 374, 428
CviKI_1 RGCY 9 cut(s) 5, 43, 85, 156, 195, 247, 364, 374, 428
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 2 cut(s) 81, 221
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 225
Eco57I CTGAAG 2 cut(s) 389, 494
EcoO109I RGGNCCY 1 cut(s) 225
FaiI YATR 8 cut(s) 39, 46, 109, 120, 297, 326, 440, 518
FaqI GGGAC 3 cut(s) 118, 211, 466
Fnu4HI GCNGC 5 cut(s) 86, 89, 245, 248, 362
Fsp4HI GCNGC 5 cut(s) 86, 89, 245, 248, 362
FspBI CTAG 2 cut(s) 375, 398
GluI GCNGC 5 cut(s) 86, 89, 245, 248, 362
HaeIII GGCC 2 cut(s) 195, 364
HgaI GACGC 1 cut(s) 164
HincII GTYRAC 1 cut(s) 179
HindII GTYRAC 1 cut(s) 179
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 2 cut(s) 479, 488
Hpy166II GTNNAC 2 cut(s) 179, 422
Hpy188I TCNGA 3 cut(s) 351, 408, 474
Hpy188III TCNNGA 3 cut(s) 80, 331, 398
Hpy8I GTNNAC 2 cut(s) 179, 422
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 3 cut(s) 95, 208, 469
HpyCH4III ACNGT 2 cut(s) 33, 183
HpyCH4IV ACGT 2 cut(s) 144, 418
HpyCH4V TGCA 6 cut(s) 61, 244, 250, 299, 340, 509
HpyF3I CTNAG 2 cut(s) 81, 221
HpySE526I ACGT 2 cut(s) 144, 418
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 5 cut(s) 93, 152, 356, 449, 489
Lsp1109I GCAGC 2 cut(s) 234, 256
LweI GCATC 2 cut(s) 349, 518
MaeI CTAG 2 cut(s) 375, 398
MaeII ACGT 2 cut(s) 144, 418
MalI GATC 1 cut(s) 66
MbiI CCGCTC 1 cut(s) 77
MboI GATC 1 cut(s) 64
MboII GAAGA 4 cut(s) 173, 323, 338, 467
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 64
MluCI AATT 4 cut(s) 96, 127, 239, 387
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 6 cut(s) 33, 216, 427, 438, 441, 490
MseI TTAA 3 cut(s) 210, 231, 411
MspA1I CMGCKG 1 cut(s) 247
MunI CAATTG 1 cut(s) 96
NdeII GATC 1 cut(s) 64
NlaIV GGNNCC 2 cut(s) 227, 365
PfeI GAWTC 1 cut(s) 488
PkrI GCNGC 5 cut(s) 87, 90, 246, 249, 363
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 225
PsiI TTATAA 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 227, 365
PspPI GGNCC 3 cut(s) 194, 225, 363
PspPPI RGGWCCY 1 cut(s) 225
PsuI RGATCY 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 210, 231, 411
SatI GCNGC 5 cut(s) 86, 89, 245, 248, 362
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 3 cut(s) 194, 225, 363
SchI GAGTC 1 cut(s) 488
SetI ASST 9 cut(s) 17, 25, 45, 147, 158, 227, 249, 421, 430
SfaNI GCATC 2 cut(s) 349, 518
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 225
Sse9I AATT 4 cut(s) 96, 127, 239, 387
SsiI CCGC 4 cut(s) 75, 86, 89, 361
SspI AATATT 1 cut(s) 202
SspMI CTAG 2 cut(s) 375, 398
TaaI ACNGT 2 cut(s) 33, 183
TaiI ACGT 2 cut(s) 147, 421
TasI AATT 4 cut(s) 96, 127, 239, 387
TauI GCSGC 3 cut(s) 88, 91, 364
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 3 cut(s) 210, 231, 411
Tru9I TTAA 3 cut(s) 210, 231, 411
TscAI CASTG 1 cut(s) 508
TseI GCWGC 2 cut(s) 244, 247
TspRI CASTG 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 225
XbaI TCTAGA 1 cut(s) 397
XspI CTAG 2 cut(s) 375, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.