Rw4G016230

Ribosomal L32p protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004051
Physical Location & Seq
Reverse (-)
37232095 .. 37233549
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016230.1

Sequence Viewer

Length: 408 bp
ATGGCGTCGAAGCTTTCCATGCTTAGGAGCGCTAGTGAGAACATGGCGTGCATTTTCGGCATCAGGAGGTGGAACCACATCGCAGCAATTCCTCCGTCGTCGGATGGGGCCATTGCCCGGACGATGATTTCATCGCCACCGTTCGCTCTGCCAGAGTATGATTGCGAGCCTGAGAAGGATAACAAAGTCGGATTCGGGTTGCCCAGTTTTCTGTTCGGTGGGTCCATGGAGCTCATGGCTGTCCCCAAGAGGAAGACTACTCCCCACAAGAGAGGCATAAGAAATGGTCCGAAAGCTCTGAAGCCTGTTCCTGTGATAATCCGATGCAGGAGCTGTGGTCGAGTCAAGCTGCCACATTATTATTGTTGCAGTGGACGTAGGGGAAGTACCGGTGAGCGGGAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L32p

Protein Analysis

135

Amino Acids

14.85

Weight (kDa)

9.93

Isoelectric Point (pI)

78.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32p PF01783 80 - 125 6.2e-10 Ribosomal L32p protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 397
AciI CCGC 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 388
AfeI AGCGCT 1 cut(s) 31
AfiI CCNNNNNNNGG 3 cut(s) 24, 117, 396
AgeI ACCGGT 1 cut(s) 389
AluBI AGCT 5 cut(s) 13, 232, 296, 333, 349
AluI AGCT 5 cut(s) 13, 232, 296, 333, 349
Alw21I GWGCWC 1 cut(s) 234
AlwNI CAGNNNCTG 1 cut(s) 333
Aor51HI AGCGCT 1 cut(s) 31
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 83, 349
AsiGI ACCGGT 1 cut(s) 389
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 108, 222, 287
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 222, 287
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 95, 336
BccI CCATC 1 cut(s) 98
BcnI CCSGG 1 cut(s) 118
BfaI CTAG 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 2 cut(s) 84, 350
BlsI GCNGC 2 cut(s) 85, 351
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 2 cut(s) 222, 287
BmgT120I GGNCC 3 cut(s) 108, 222, 287
BmiI GGNNCC 3 cut(s) 74, 109, 223
BmrFI CCNGG 1 cut(s) 118
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 69, 314
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 322, 352
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 117, 396
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 3 cut(s) 24, 117, 396
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 95, 336
BshFI GGCC 1 cut(s) 110
BshTI ACCGGT 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 2 cut(s) 118, 390
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 24, 117, 396
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 1 cut(s) 397
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 3 cut(s) 74, 109, 223
BsrBI CCGCTC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 49, 167
BstDEI CTNAG 2 cut(s) 23, 171
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstMWI GCNNNNNNNGC 2 cut(s) 19, 57
BstSCI CCNGG 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 95, 336
BstV2I GAAGAC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 225
BtgZI GCGATG 2 cut(s) 64, 117
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 49, 167
CaiI CAGNNNCTG 1 cut(s) 333
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 3 cut(s) 108, 222, 287
Csp6I GTAC 1 cut(s) 387
CspAI ACCGGT 1 cut(s) 389
CviAII CATG 4 cut(s) 19, 43, 226, 235
CviJI RGCY 9 cut(s) 13, 110, 169, 232, 239, 296, 304, 333, 349
CviKI_1 RGCY 9 cut(s) 13, 110, 169, 232, 239, 296, 304, 333, 349
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 23, 171
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 222, 287
Eco47III AGCGCT 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 4 cut(s) 22, 46, 229, 238
FaiI YATR 7 cut(s) 20, 44, 159, 227, 236, 278, 406
FaqI GGGAC 1 cut(s) 227
FatI CATG 4 cut(s) 18, 42, 225, 234
FauI CCCGC 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 84, 350
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 84, 350
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 31
GluI GCNGC 2 cut(s) 84, 350
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 118, 390
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 22, 46, 229, 238
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 2 cut(s) 192, 342
HpaII CCGG 2 cut(s) 118, 390
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 1 cut(s) 374
Hpy188I TCNGA 5 cut(s) 103, 191, 291, 300, 323
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 374
Hpy99I CGWCG 3 cut(s) 10, 100, 103
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 3 cut(s) 51, 327, 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 57
HpyF3I CTNAG 2 cut(s) 23, 171
HpySE526I ACGT 1 cut(s) 376
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 22, 46, 229, 238
HspAI GCGC 1 cut(s) 30
LmnI GCTCC 3 cut(s) 27, 229, 330
LpnPI CCDG 9 cut(s) 49, 131, 165, 183, 217, 313, 318, 324, 403
Lsp1109I GCAGC 2 cut(s) 95, 336
LweI GCATC 2 cut(s) 69, 314
MaeI CTAG 1 cut(s) 33
MaeII ACGT 1 cut(s) 376
MbiI CCGCTC 1 cut(s) 397
MboII GAAGA 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 1 cut(s) 87
MlyI GAGTC 1 cut(s) 351
MmeI TCCRAC 2 cut(s) 81, 169
MnlI CCTC 5 cut(s) 60, 102, 243, 266, 394
MspI CCGG 2 cut(s) 118, 390
MspR9I CCNGG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 19, 57
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 1 cut(s) 225
NlaIII CATG 4 cut(s) 22, 46, 229, 238
NlaIV GGNNCC 3 cut(s) 74, 109, 223
PfeI GAWTC 1 cut(s) 192
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 2 cut(s) 85, 351
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 74, 109, 223
PspPI GGNCC 3 cut(s) 108, 222, 287
PstNI CAGNNNCTG 1 cut(s) 333
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SacI GAGCTC 1 cut(s) 234
SatI GCNGC 2 cut(s) 84, 350
Sau96I GGNCC 3 cut(s) 108, 222, 287
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 234
SetI ASST 8 cut(s) 15, 71, 234, 298, 335, 351, 379, 405
SfaNI GCATC 2 cut(s) 69, 314
SinI GGWCC 2 cut(s) 222, 287
Sse9I AATT 1 cut(s) 87
SsiI CCGC 1 cut(s) 397
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 141
TaiI ACGT 1 cut(s) 379
TaqI TCGA 2 cut(s) 8, 340
TasI AATT 1 cut(s) 87
TfiI GAWTC 1 cut(s) 192
TscAI CASTG 1 cut(s) 376
TseI GCWGC 2 cut(s) 83, 349
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 2 cut(s) 222, 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.