Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003437

Physical Location & Seq

Genomic Coordinates Reverse (-)

52387018 .. 52391263

Gene Structure 4246 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G028360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGCTGTCTTCTATGGAGGATGGGGAGATCAGCGTATCAGCGTACGACACCGCCTGGGTTGCTTTGGTTGAAAATGTAAATGAAACTGATTCACCTCAATTCCCTTCCAGCCTCGAATGGATTGCCAACAACCAACTCGAAGATGGTTCTTGGGGCTACGAGGACATCTACTCCGCCCACGATCGGATTATCAGCACCCTCGCCTGCGTCGTCGCTTTGAAGTCGTGGAATCTCCACCCCGACAAATGTGACAAAGGAATGAAGTTTTTCAAGGAAAACCTAAACAAGCTGGAAGAAGAGAATATCGAACACATGCCTATAGGGTTCGAAGTTGCGTTTCCCTCTGTTCTGGAAATAGCTCGGAGCTTAAACCTTGAAGTGCCTGATGACTCTCCTGTGTTGCATAAGATCTATGCCTGCAGAAACTTAAAACTAACCAAGATACCCAAGGACATACTGCACAAAGTGGCCACAACGCTTCTTCATAGCGTGGAAGGGATGGCAGGTGTCGACTGGGAAAAGCTTCTGAAACTCCAGTCCCAAGACGGCTCGTTTTTGTTCTCTCCGGCGTCCACTGCCTACGCTGCTCAGCAGACCAAGGACGGAAAATGCATGTCGTATCTGTCCAGAGCAGTCCGCAAGTTCAATGGAGGAGTCCCTAATGTTTACCCAGTCGACTTGTTCGAACACATGTGGGCTGTGGATCGGTTGCAGAGGTTAGGATTGTGCAGATACTTCGAGCCGGAGATTAAGGAGTGTATGAACTACGTTGCCAAGTACTGGACTGAGAAAGGAATTTGCTGGGCGAGAAACTCAGAGGTTCAAGACATCGATGACACAGCGATGGGGTTCAGACTGCTCCGGTTGCATGGGCACAAAGTGTCAGCCGATGTGTTTAAGCATTTTAAGAAAGGGGACGAGTTTTTCTGCTTTGCGGGACAATCGAACCAGGCTGTGACCGGAATGTACAACTTGTATAGGGCAAGTCAGGTGGCGTTACCGGGAGAGAAGATCCTCTATGAAGCCAAGGAGTTCTCGACAAAGTTCCTTAGGGAGAAGCAAGCTTCAAACGAGCTTCTAGACAAGTGGATCATAATGAAGGACTTGCCCGGAGAGGTGGGATATGCTCTTGATGTTCCATGGTATGCCAGCTTACCTCGACTAGAGACTAAATTCTATATCGAACAATATGGTGGTGAAGATGACGTATGGATTGGCAAGACTCTCTACAGTATGCCCTACGTTAACAATAATGTGTATCTTGAGCTGGCAAAGCTAGACTTCAACAATTGCCAAGCACTACATCTCAGTGAATGGGACAACCTACAAAAGTGGTACGGAGAATGGAAAATTGGAGGCTATGGACTAAGCAGAAAAAGTCTCCTCATGGCTTATTTTGTTGCCGCAGCAAGTATTTTTGAACCTGAAAGAGCAAATGAGCGACTCGCATGGGCTAAGACGGCCTGCTTAGTTGACACGATTGAGGCTTATTTTAAGAGAGAAACTTCTCGTGAAGATAAGAAGGCATTTGTTGATGAGTTCAAAAACCTTTCAAATATGCGAGAGTATGAAGTTGCAAGGAGGTCGAATAGGAACAGGACTGCCGGGCAAGGAATTGTTGGGGCTTTGTTGGCGACGCTAAGCCAACTCTCATTGGGCACAATGGTGCTACATGGCCAAGACATTACCCAATCTCTACGTCAATCTTGGGAGAAGTGGCTACTGAAGTGGCTAGAGATAGGGGGAAGGCATCAAGATGAAGCTGAGCTACTAGTAGAAACGATAAATCAAACCGCTGGCCTTTCACTAACTGATGGGTTGTTGTCCAATAATCCAGAACATGAGCTGCTTTTTAGCCTGACTAACAAAGTTTGCAATCAACTTCGTTTTTGCCAAAACCAAAAGCACAAGATGAACAATAATGGCAGCTGCGGAACAAAAATGAACATGACTGCCAAAGCAATAGAATCAGACATGCAGCAGCTTGTGGAATTGGTGCTAAAATCTTCGGATGGCATAGAATCCTTAATCAAGCAAAGCTTTTTCTCAGTAGCAAGGAGCTTTTATTATTCGACCTACTGTGACCCTAAAATTATTAGCCACCACATTGACAAAGTACTCTTCGAGAGAGCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0001101 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009905 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016853 GO:0016872 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

712

Amino Acids

81.35

Weight (kDa)

5.75

Isoelectric Point (pI)

40.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	171 - 377	1.9e-48	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	455 - 578	9.3e-08	Terpene synthase family, metal binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003437)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02780
fragaria_vesca	FvH4_2g23400 FvH4_2g23400 FvH4_2g23400 FvH4_2g23420 FvH4_2g23440 FvH4_2g23440 FvH4_2g23440
malus_domestica	MD03G1263500.v1.1 MD11G1208700.v1.1 MD11G1284300.v1.1
prunus_persica	Prupe.8G239900_v2.0.a1
pyrus_communis	pycom11g25170
rosa_chinensis	RchiOBHm_Chr6g0290871 RchiOBHm_Chr6g0290941
rosa_laevigata	RLG00000012165
rosa_multiflora	Rmu_sc0001577.1_g000003
rosa_roxburghii	Rroxscaffold_7G00176530 Rroxscaffold_7G00176670
rosa_rugosa	Rorug06G0215400 Rorug06G0215500 Rorug06G0215600 Rorug06G0215700 Rorug06G0215900 Rorug06G0216000 Rorug06G0216000
rosa_samantha	Rh6AG325900 Rh6AG326200 Rh6BG333600 Rh6BG333800 Rh6CG339300 Rh6CG339700 Rh6DG326600 Rh6DG327100
rosa_wichuraiana	Rw6G028340 Rw6G028360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	494
Acc36I	ACCTGC	1 cut(s)	494
AccB7I	CCANNNNNTGG	1 cut(s)	780
AccI	GTMKAC	2 cut(s)	510, 675
AciI	CCGC	7 cut(s)	51, 174, 637, 935, 1404, 1794, 1932
AclWI	GGATC	3 cut(s)	711, 1006, 1097
AcoI	YGGCCR	2 cut(s)	468, 1675
AcsI	RAATTY	2 cut(s)	795, 1172
AcuI	CTGAAG	1 cut(s)	1745
AcyI	GRCGYC	1 cut(s)	569
AdeI	CACNNNGTG	2 cut(s)	466, 880
AfaI	GTAC	5 cut(s)	44, 779, 968, 1337, 2118
AfiI	CCNNNNNNNGG	2 cut(s)	183, 780
AflIII	ACRYGT	1 cut(s)	690
AhlI	ACTAGT	1 cut(s)	1771
AjnI	CCWGG	2 cut(s)	53, 948
AjuI	GAANNNNNNNTTGG	2 cut(s)	1335, 1367
AleI	CACNNNNGTG	1 cut(s)	1664
Alw26I	GTCTC	2 cut(s)	1160, 1385
AlwI	GGATC	3 cut(s)	711, 1006, 1097
AoxI	GGCC	4 cut(s)	468, 1461, 1675, 1798
ApeKI	GCWGC	7 cut(s)	584, 1406, 1846, 1926, 1929, 1978, 1981
ApoI	RAATTY	2 cut(s)	795, 1172
Asp700I	GAANNNNTTC	2 cut(s)	266, 522
AsuC2I	CCSGG	3 cut(s)	1002, 1110, 1606
AsuHPI	GGTGA	2 cut(s)	84, 1208
AsuII	TTCGAA	2 cut(s)	327, 684
AxyI	CCTNAGG	1 cut(s)	1049
BaeGI	GKGCMC	2 cut(s)	876, 1661
BalI	TGGCCA	2 cut(s)	470, 1677
BarI	GAAGNNNNNNTAC	2 cut(s)	1752, 1784
BauI	CACGAG	1 cut(s)	1509
BbvI	GCAGC	7 cut(s)	571, 1418, 1833, 1916, 1938, 1990, 1993
BccI	CCATC	6 cut(s)	14, 137, 493, 838, 1808, 2006
BceAI	ACGGC	2 cut(s)	562, 1476
BcgI	CGANNNNNNTGC	6 cut(s)	104, 138, 718, 752, 1566, 1600
BciT130I	CCWGG	2 cut(s)	55, 950
BcnI	CCSGG	3 cut(s)	1002, 1110, 1606
BcoDI	GTCTC	2 cut(s)	1160, 1385
BcuI	ACTAGT	1 cut(s)	1771
BfaI	CTAG	5 cut(s)	1079, 1163, 1277, 1733, 1772
BfmI	CTRYAG	3 cut(s)	318, 418, 1228
BfuAI	ACCTGC	1 cut(s)	494
BglII	AGATCT	1 cut(s)	408
BisI	GCNGC	8 cut(s)	585, 1404, 1407, 1847, 1927, 1930, 1979, 1982
BlpI	GCTNAGC	3 cut(s)	588, 1640, 1764
BlsI	GCNGC	8 cut(s)	586, 1405, 1408, 1848, 1928, 1931, 1980, 1983
BmcAI	AGTACT	2 cut(s)	779, 2118
Bme1390I	CCNGG	5 cut(s)	55, 950, 1002, 1110, 1606
BmrFI	CCNGG	5 cut(s)	55, 950, 1002, 1110, 1606
BmrI	ACTGGG	2 cut(s)	523, 665
BmsI	GCATC	1 cut(s)	1759
BmuI	ACTGGG	2 cut(s)	523, 665
BpmI	CTGGAG	1 cut(s)	518
Bpu1102I	GCTNAGC	3 cut(s)	588, 1640, 1764
Bpu14I	TTCGAA	2 cut(s)	327, 684
BpuEI	CTTGAG	1 cut(s)	1283
BpuMI	CCSGG	3 cut(s)	1002, 1110, 1606
Bsa29I	ATCGAT	1 cut(s)	831
BsaHI	GRCGYC	1 cut(s)	569
BsaJI	CCNNGG	5 cut(s)	54, 447, 597, 1026, 1139
BsaWI	WCCGGW	2 cut(s)	861, 959
BsaXI	ACNNNNNCTCC	2 cut(s)	155, 185
Bsc4I	CCNNNNNNNGG	2 cut(s)	183, 780
Bse1I	ACTGG	4 cut(s)	518, 535, 671, 785
Bse21I	CCTNAGG	1 cut(s)	1049
BseBI	CCWGG	2 cut(s)	55, 950
BseCI	ATCGAT	1 cut(s)	831
BseDI	CCNNGG	5 cut(s)	54, 447, 597, 1026, 1139
BseGI	GGATG	3 cut(s)	25, 504, 2017
BseLI	CCNNNNNNNGG	2 cut(s)	183, 780
BseMII	CTCAG	6 cut(s)	602, 777, 828, 1321, 1755, 2061
BseNI	ACTGG	4 cut(s)	518, 535, 671, 785
BseRI	GAGGAG	2 cut(s)	666, 1373
BseSI	GKGCMC	2 cut(s)	876, 1661
BseXI	GCAGC	7 cut(s)	571, 1418, 1833, 1916, 1938, 1990, 1993
BseYI	CCCAGC	1 cut(s)	801
BsgI	GTGCAG	2 cut(s)	443, 748
Bsh1285I	CGRYCG	1 cut(s)	184
BshFI	GGCC	4 cut(s)	470, 1463, 1677, 1800
BshVI	ATCGAT	1 cut(s)	831
BsiEI	CGRYCG	1 cut(s)	184
BsiSI	CCGG	7 cut(s)	566, 743, 862, 960, 1001, 1110, 1605
BsiWI	CGTACG	1 cut(s)	42
BslFI	GGGAC	5 cut(s)	523, 641, 929, 951, 1331
BslI	CCNNNNNNNGG	2 cut(s)	183, 780
BsmAI	GTCTC	2 cut(s)	1160, 1385
BsmFI	GGGAC	5 cut(s)	523, 641, 929, 951, 1331
BsnI	GGCC	4 cut(s)	470, 1463, 1677, 1800
Bsp119I	TTCGAA	2 cut(s)	327, 684
Bsp1286I	GDGCHC	2 cut(s)	876, 1661
Bsp1407I	TGTACA	1 cut(s)	966
Bsp143I	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
Bsp1720I	GCTNAGC	3 cut(s)	588, 1640, 1764
Bsp19I	CCATGG	1 cut(s)	1139
BspACI	CCGC	7 cut(s)	51, 174, 637, 935, 1404, 1794, 1932
BspANI	GGCC	4 cut(s)	470, 1463, 1677, 1800
BspCNI	CTCAG	6 cut(s)	601, 778, 827, 1320, 1756, 2060
BspDI	ATCGAT	1 cut(s)	831
BspMAI	CTGCAG	1 cut(s)	422
BspMI	ACCTGC	1 cut(s)	494
BspPI	GGATC	3 cut(s)	711, 1006, 1097
BspT104I	TTCGAA	2 cut(s)	327, 684
BsrGI	TGTACA	1 cut(s)	966
BsrI	ACTGG	4 cut(s)	518, 535, 671, 785
BssECI	CCNNGG	5 cut(s)	54, 447, 597, 1026, 1139
BssMI	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
BssNI	GRCGYC	1 cut(s)	569
BssSI	CACGAG	1 cut(s)	1509
BssT1I	CCWWGG	4 cut(s)	447, 597, 1026, 1139
Bst2BI	CACGAG	1 cut(s)	1509
Bst2UI	CCWGG	2 cut(s)	55, 950
Bst4CI	ACNGT	2 cut(s)	1232, 2081
Bst6I	CTCTTC	2 cut(s)	291, 2126
BstACI	GRCGYC	1 cut(s)	569
BstAUI	TGTACA	1 cut(s)	966
BstBI	TTCGAA	2 cut(s)	327, 684
BstC8I	GCNNGC	7 cut(s)	205, 418, 1062, 1150, 1269, 1465, 1798
BstDSI	CCRYGG	1 cut(s)	1139
BstF5I	GGATG	3 cut(s)	25, 504, 2017
BstKTI	GATC	6 cut(s)	30, 184, 411, 706, 1014, 1092
BstMAI	GTCTC	2 cut(s)	1160, 1385
BstMBI	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
BstMCI	CGRYCG	1 cut(s)	184
BstMWI	GCNNNNNNNGC	7 cut(s)	59, 575, 584, 865, 1273, 1460, 1631
BstNI	CCWGG	2 cut(s)	55, 950
BstNSI	RCATGY	4 cut(s)	316, 616, 694, 1978
BstSCI	CCNGG	5 cut(s)	53, 948, 1000, 1108, 1604
BstSFI	CTRYAG	3 cut(s)	318, 418, 1228
BstSLI	GKGCMC	2 cut(s)	876, 1661
BstV1I	GCAGC	7 cut(s)	571, 1418, 1833, 1916, 1938, 1990, 1993
BstX2I	RGATCY	2 cut(s)	408, 1011
BstYI	RGATCY	2 cut(s)	408, 1011
Bsu15I	ATCGAT	1 cut(s)	831
Bsu36I	CCTNAGG	1 cut(s)	1049
BsuRI	GGCC	4 cut(s)	470, 1463, 1677, 1800
BsuTUI	ATCGAT	1 cut(s)	831
BtgI	CCRYGG	1 cut(s)	1139
BtgZI	GCGATG	1 cut(s)	857
BtsCI	GGATG	3 cut(s)	25, 504, 2017
BtsI	GCAGTG	1 cut(s)	573
BtsIMutI	CAGTG	2 cut(s)	573, 1315
BveI	ACCTGC	1 cut(s)	494
Cac8I	GCNNGC	7 cut(s)	205, 418, 1062, 1150, 1269, 1465, 1798
ClaI	ATCGAT	1 cut(s)	831
CseI	GACGC	3 cut(s)	196, 558, 1645
Csp6I	GTAC	5 cut(s)	43, 778, 967, 1336, 2117
CspCI	CAANNNNNGTGG	2 cut(s)	972, 1007
CviQI	GTAC	5 cut(s)	43, 778, 967, 1336, 2117
DpnI	GATC	6 cut(s)	29, 183, 410, 705, 1013, 1091
DpnII	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
DraIII	CACNNNGTG	2 cut(s)	466, 880
EaeI	YGGCCR	2 cut(s)	468, 1675
Eam1104I	CTCTTC	2 cut(s)	291, 2126
EarI	CTCTTC	2 cut(s)	291, 2126
EciI	GGCGGA	1 cut(s)	163
Eco130I	CCWWGG	4 cut(s)	447, 597, 1026, 1139
Eco57I	CTGAAG	1 cut(s)	1745
Eco81I	CCTNAGG	1 cut(s)	1049
EcoRII	CCWGG	2 cut(s)	53, 948
EcoT14I	CCWWGG	4 cut(s)	447, 597, 1026, 1139
EcoT22I	ATGCAT	1 cut(s)	614
ErhI	CCWWGG	4 cut(s)	447, 597, 1026, 1139
FalI	AAGNNNNNCTT	4 cut(s)	1265, 1297, 2024, 2056
FaqI	GGGAC	5 cut(s)	523, 641, 929, 951, 1331
FauI	CCCGC	1 cut(s)	928
FblI	GTMKAC	2 cut(s)	510, 675
Fnu4HI	GCNGC	8 cut(s)	585, 1404, 1407, 1847, 1927, 1930, 1979, 1982
FokI	GGATG	3 cut(s)	32, 511, 2024
Fsp4HI	GCNGC	8 cut(s)	585, 1404, 1407, 1847, 1927, 1930, 1979, 1982
FspBI	CTAG	5 cut(s)	1079, 1163, 1277, 1733, 1772
GluI	GCNGC	8 cut(s)	585, 1404, 1407, 1847, 1927, 1930, 1979, 1982
GsaI	CCCAGC	1 cut(s)	805
GsuI	CTGGAG	1 cut(s)	518
HaeIII	GGCC	4 cut(s)	470, 1463, 1677, 1800
HapII	CCGG	7 cut(s)	566, 743, 862, 960, 1001, 1110, 1605
HgaI	GACGC	3 cut(s)	196, 558, 1645
Hin1I	GRCGYC	1 cut(s)	569
HincII	GTYRAC	4 cut(s)	511, 676, 1246, 1474
HindII	GTYRAC	4 cut(s)	511, 676, 1246, 1474
HindIII	AAGCTT	3 cut(s)	521, 1062, 2038
HinfI	GANTC	8 cut(s)	89, 229, 389, 654, 1222, 1443, 1967, 2021
HpaI	GTTAAC	1 cut(s)	1246
HpaII	CCGG	7 cut(s)	566, 743, 862, 960, 1001, 1110, 1605
HphI	GGTGA	2 cut(s)	84, 1208
Hpy166II	GTNNAC	6 cut(s)	511, 573, 667, 676, 1246, 1474
Hpy188I	TCNGA	7 cut(s)	186, 363, 528, 817, 854, 1972, 2011
Hpy8I	GTNNAC	6 cut(s)	511, 573, 667, 676, 1246, 1474
Hpy99I	CGWCG	3 cut(s)	212, 215, 1639
HpyAV	CCTTC	5 cut(s)	114, 488, 1093, 1516, 1740
HpyCH4III	ACNGT	2 cut(s)	1232, 2081
HpyCH4IV	ACGT	4 cut(s)	768, 1206, 1242, 1699
HpyF10VI	GCNNNNNNNGC	7 cut(s)	59, 575, 584, 865, 1273, 1460, 1631
HpySE526I	ACGT	4 cut(s)	768, 1206, 1242, 1699
Hsp92I	GRCGYC	1 cut(s)	569
KspAI	GTTAAC	1 cut(s)	1246
Kzo9I	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
LmnI	GCTCC	3 cut(s)	363, 864, 2058
Lsp1109I	GCAGC	7 cut(s)	571, 1418, 1833, 1916, 1938, 1990, 1993
LweI	GCATC	1 cut(s)	1759
MaeI	CTAG	5 cut(s)	1079, 1163, 1277, 1733, 1772
MaeII	ACGT	4 cut(s)	768, 1206, 1242, 1699
MaeIII	GTNAC	4 cut(s)	248, 955, 996, 2081
MalI	GATC	6 cut(s)	29, 183, 410, 705, 1013, 1091
MboI	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
MboII	GAAGA	9 cut(s)	152, 305, 308, 473, 1021, 1211, 1526, 1998, 2113
MfeI	CAATTG	1 cut(s)	1288
MflI	RGATCY	2 cut(s)	408, 1011
MhlI	GDGCHC	2 cut(s)	876, 1661
MlsI	TGGCCA	2 cut(s)	470, 1677
MluCI	AATT	9 cut(s)	98, 795, 1172, 1288, 1350, 1614, 1991, 2091, 2133
MluNI	TGGCCA	2 cut(s)	470, 1677
MlyI	GAGTC	4 cut(s)	383, 663, 1216, 1437
Mox20I	TGGCCA	2 cut(s)	470, 1677
Mph1103I	ATGCAT	1 cut(s)	614
MroXI	GAANNNNTTC	2 cut(s)	266, 522
MscI	TGGCCA	2 cut(s)	470, 1677
MseI	TTAA	8 cut(s)	368, 428, 750, 897, 906, 1245, 1494, 2027
MslI	CAYNNNNRTG	4 cut(s)	842, 1308, 1664, 1755
Msp20I	TGGCCA	2 cut(s)	470, 1677
MspA1I	CMGCKG	2 cut(s)	1796, 1929
MspI	CCGG	7 cut(s)	566, 743, 862, 960, 1001, 1110, 1605
MspR9I	CCNGG	5 cut(s)	55, 950, 1002, 1110, 1606
MunI	CAATTG	1 cut(s)	1288
MvaI	CCWGG	2 cut(s)	55, 950
MwoI	GCNNNNNNNGC	7 cut(s)	59, 575, 584, 865, 1273, 1460, 1631
NciI	CCSGG	3 cut(s)	1002, 1110, 1606
NcoI	CCATGG	1 cut(s)	1139
NdeII	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
NmuCI	GTSAC	3 cut(s)	248, 955, 2081
NsiI	ATGCAT	1 cut(s)	614
NspI	RCATGY	4 cut(s)	316, 616, 694, 1978
NspV	TTCGAA	2 cut(s)	327, 684
OliI	CACNNNNGTG	1 cut(s)	1664
PaqCI	CACCTGC	1 cut(s)	494
PciI	ACATGT	1 cut(s)	690
PcsI	WCGNNNNNNNCGW	2 cut(s)	207, 681
PdmI	GAANNNNTTC	2 cut(s)	266, 522
PfeI	GAWTC	4 cut(s)	89, 229, 1967, 2021
Pfl23II	CGTACG	1 cut(s)	42
PflMI	CCANNNNNTGG	1 cut(s)	780
PkrI	GCNGC	8 cut(s)	586, 1405, 1408, 1848, 1928, 1931, 1980, 1983
Ple19I	CGATCG	1 cut(s)	184
PleI	GAGTC	4 cut(s)	383, 662, 1216, 1437
PpsI	GAGTC	4 cut(s)	383, 662, 1216, 1437
PscI	ACATGT	1 cut(s)	690
Psp6I	CCWGG	2 cut(s)	53, 948
PspFI	CCCAGC	1 cut(s)	801
PspGI	CCWGG	2 cut(s)	53, 948
PspLI	CGTACG	1 cut(s)	42
PstI	CTGCAG	1 cut(s)	422
PsuI	RGATCY	2 cut(s)	408, 1011
PvuI	CGATCG	1 cut(s)	184
PvuII	CAGCTG	1 cut(s)	1929
RsaI	GTAC	5 cut(s)	44, 779, 968, 1337, 2118
RsaNI	GTAC	5 cut(s)	43, 778, 967, 1336, 2117
RseI	CAYNNNNRTG	4 cut(s)	842, 1308, 1664, 1755
SalI	GTCGAC	2 cut(s)	509, 674
SaqAI	TTAA	8 cut(s)	368, 428, 750, 897, 906, 1245, 1494, 2027
SatI	GCNGC	8 cut(s)	585, 1404, 1407, 1847, 1927, 1930, 1979, 1982
Sau3AI	GATC	6 cut(s)	27, 181, 408, 703, 1011, 1089
ScaI	AGTACT	2 cut(s)	779, 2118
SchI	GAGTC	4 cut(s)	383, 663, 1216, 1437
ScrFI	CCNGG	5 cut(s)	55, 950, 1002, 1110, 1606
SduI	GDGCHC	2 cut(s)	876, 1661
SfaNI	GCATC	1 cut(s)	1759
SfcI	CTRYAG	3 cut(s)	318, 418, 1228
SfuI	TTCGAA	2 cut(s)	327, 684
SmiMI	CAYNNNNRTG	4 cut(s)	842, 1308, 1664, 1755
SmlI	CTYRAG	1 cut(s)	1262
SmoI	CTYRAG	1 cut(s)	1262
SpeI	ACTAGT	1 cut(s)	1771
Sse9I	AATT	9 cut(s)	98, 795, 1172, 1288, 1350, 1614, 1991, 2091, 2133
SsiI	CCGC	7 cut(s)	51, 174, 637, 935, 1404, 1794, 1932
SspMI	CTAG	5 cut(s)	1079, 1163, 1277, 1733, 1772
StyD4I	CCNGG	5 cut(s)	53, 948, 1000, 1108, 1604
StyI	CCWWGG	4 cut(s)	447, 597, 1026, 1139
TaaI	ACNGT	2 cut(s)	1232, 2081
TaiI	ACGT	4 cut(s)	771, 1209, 1245, 1702
TasI	AATT	9 cut(s)	98, 795, 1172, 1288, 1350, 1614, 1991, 2091, 2133
TatI	WGTACW	3 cut(s)	777, 966, 2116
TauI	GCSGC	1 cut(s)	1406
TfiI	GAWTC	4 cut(s)	89, 229, 1967, 2021
Tru1I	TTAA	8 cut(s)	368, 428, 750, 897, 906, 1245, 1494, 2027
Tru9I	TTAA	8 cut(s)	368, 428, 750, 897, 906, 1245, 1494, 2027
TscAI	CASTG	2 cut(s)	580, 1315
TseFI	GTSAC	3 cut(s)	248, 955, 2081
TseI	GCWGC	7 cut(s)	584, 1406, 1846, 1926, 1929, 1978, 1981
Tsp45I	GTSAC	3 cut(s)	248, 955, 2081
TspGWI	ACGGA	2 cut(s)	618, 1353
TspRI	CASTG	2 cut(s)	580, 1315
Van91I	CCANNNNNTGG	1 cut(s)	780
XapI	RAATTY	2 cut(s)	795, 1172
XbaI	TCTAGA	1 cut(s)	1078
XceI	RCATGY	4 cut(s)	316, 616, 694, 1978
XcmI	CCANNNNNNNNNTGG	1 cut(s)	140
XmiI	GTMKAC	2 cut(s)	510, 675
XmnI	GAANNNNTTC	2 cut(s)	266, 522
XspI	CTAG	5 cut(s)	1079, 1163, 1277, 1733, 1772
ZrmI	AGTACT	2 cut(s)	779, 2118
Zsp2I	ATGCAT	1 cut(s)	614

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.