AT1G04990

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002970
Physical Location & Seq
Reverse (-)
1418612 .. 1421863
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04990.3

Sequence Viewer

Length: 1215 bp
ATGCGAACCCCCATGTCAGACACTCAGCATGTACAGAGCTCTTTGGTGTCGATTCGATCTTCCGACAAAATCGAAGATGCCTTTAGGAAGATGAAAGTAAATGAGACCGGCGTGGAGGAACTGAATCCGTATCCAGATCGTCCCGGTGAAAGAGATTGCCAATTCTATTTAAGAACTGGTCTCTGTGGCTATGGAAGCAGTTGCCGTTACAATCATCCTACTCATCTTCCACAGGATGTTGCTTATTACAAGGAGGAGCTCCCTGAGAGAATTGGACAGCCGGATTGTGAGTATTTTCTGAAGACAGGAGCTTGTAAGTACGGCCCAACTTGTAAATATCATCATCCAAAGGACAGGAACGGTGCACAACCTGTAATGTTCAATGTTATCGGTTTACCTATGCGTCTGGGTGAGAAGCCATGTCCATATTACCTGCGAACGGGAACTTGCAGATTCGGAGTTGCTTGCAAATTCCACCATCCTCAACCTGATAATGGACATTCTACTGCATATGGAATGTCTAGCTTTCCTGCTGCAGATTTACGATATGCTAGTGGTTTAACAATGATGTCTACATATGGAACTTTGCCTCGTCCGCAAGTTCCCCAGTCCTATGTGCCAATCCTGGTTTCACCCTCTCAAGGCTTTTTACCTCCTCAAGGCTGGGCCCCTTACATGGCTGCATCTAACTCCATGTACAATGTGAAGAATCAACCTTATTACTCCGGTAGTAGTGCATCCATGGCCATGGCTGTGGCATTGAACCGTGGTTTGTCAGAAAGTTCTGACCAACCAGAATGTCGGTTTTTTATGAACACCGGGACATGTAAATACGGAGACGATTGCAAATACAGCCATCCCGGAGTAAGGATTTCACAACCACCACCAAGTCTTATAAACCCTTTTGTTCTTCCAGCGAGACCTGGACAACCAGCTTGTGGTAACTTCAGGTCTTATGGATTCTGCAAGTTTGGACCAAACTGCAAATTCGACCACCCTATGCTGCCATATCCGGGCCTGACCATGGCTACTTCTCTGCCTACTCCTTTTGCTTCACCTGTTACAACCCATCAGAGGATCTCACCAACACCAAACCGCTCTGATTCAAAGTCTCTTTCTAATGGTAAACCTGATGTGAAGAAAGAAAGCTCAGAGACTGAGAAACCAGACAATGGAGAAGTGCAGGATTTGTCTGAGGATGCGTCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

404

Amino Acids

44.7

Weight (kDa)

8.4

Isoelectric Point (pI)

56.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 16 - 36 1.1e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 56 - 81 1.5e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 182 - 206 2.3e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf_CCCH_4 PF18345 186 - 205 4.1e-08 Zinc finger domain
zf-CCCH PF00642 228 - 253 6e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
Acc36I ACCTGC 1 cut(s) 441
AccB7I CCANNNNNTGG 2 cut(s) 938, 1172
AccBSI CCGCTC 1 cut(s) 1098
AccI GTMKAC 1 cut(s) 572
AciI CCGC 2 cut(s) 596, 1096
AclWI GGATC 1 cut(s) 1085
AcoI YGGCCR 1 cut(s) 744
AcsI RAATTY 2 cut(s) 470, 986
AcuI CTGAAG 2 cut(s) 320, 931
AfaI GTAC 3 cut(s) 33, 320, 698
AflIII ACRYGT 1 cut(s) 824
AgsI TTSAA 3 cut(s) 382, 763, 1107
AjnI CCWGG 2 cut(s) 624, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 6 cut(s) 39, 259, 311, 525, 935, 1149
AluI AGCT 6 cut(s) 39, 259, 311, 525, 935, 1149
Alw21I GWGCWC 3 cut(s) 41, 261, 367
Alw26I GTCTC 6 cut(s) 98, 185, 831, 913, 1116, 1148
Alw44I GTGCAC 1 cut(s) 363
AlwI GGATC 1 cut(s) 1085
AlwNI CAGNNNCTG 1 cut(s) 1157
AoxI GGCC 4 cut(s) 322, 666, 744, 1015
ApaI GGGCCC 1 cut(s) 670
ApaLI GTGCAC 1 cut(s) 363
ApeKI GCWGC 3 cut(s) 533, 680, 1003
ApoI RAATTY 2 cut(s) 470, 986
AspS9I GGNCC 5 cut(s) 323, 666, 667, 974, 1015
AsuC2I CCSGG 4 cut(s) 144, 820, 861, 1014
AsuHPI GGTGA 6 cut(s) 158, 422, 624, 1047, 1074, 1200
AvaII GGWCC 1 cut(s) 974
BaeGI GKGCMC 2 cut(s) 367, 670
BalI TGGCCA 1 cut(s) 746
BanII GRGCYC 3 cut(s) 41, 261, 670
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 3 cut(s) 41, 261, 367
BbvI GCAGC 3 cut(s) 520, 667, 990
BccI CCATC 3 cut(s) 486, 864, 1077
BceAI ACGGC 2 cut(s) 189, 337
BciT130I CCWGG 2 cut(s) 626, 924
BciVI GTATCC 1 cut(s) 141
BcnI CCSGG 4 cut(s) 144, 820, 861, 1014
BcoDI GTCTC 6 cut(s) 98, 185, 831, 913, 1116, 1148
BfaI CTAG 2 cut(s) 522, 552
BfmI CTRYAG 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 441
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 3 cut(s) 534, 681, 1004
BlsI GCNGC 3 cut(s) 535, 682, 1005
Bme1390I CCNGG 6 cut(s) 144, 626, 820, 861, 924, 1014
Bme18I GGWCC 1 cut(s) 974
BmgT120I GGNCC 5 cut(s) 323, 666, 667, 974, 1015
BmiI GGNNCC 2 cut(s) 668, 669
BmrFI CCNGG 6 cut(s) 144, 626, 820, 861, 924, 1014
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 4 cut(s) 67, 692, 746, 1189
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 624, 642
BpuMI CCSGG 4 cut(s) 144, 820, 861, 1014
BsaI GGTCTC 3 cut(s) 98, 185, 913
BsaJI CCNNGG 4 cut(s) 741, 747, 766, 1023
BsaWI WCCGGW 1 cut(s) 725
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 181, 607
BseBI CCWGG 2 cut(s) 626, 924
BseDI CCNNGG 4 cut(s) 741, 747, 766, 1023
BseGI GGATG 7 cut(s) 214, 241, 343, 478, 737, 856, 1204
BseMII CTCAG 5 cut(s) 38, 255, 1149, 1164, 1185
BseNI ACTGG 2 cut(s) 181, 607
BseRI GAGGAG 2 cut(s) 269, 645
BseSI GKGCMC 2 cut(s) 367, 670
BseXI GCAGC 3 cut(s) 520, 667, 990
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 1202
BshFI GGCC 4 cut(s) 324, 668, 746, 1017
BsiHKAI GWGCWC 3 cut(s) 41, 261, 367
BsiSI CCGG 7 cut(s) 108, 144, 281, 726, 819, 861, 1013
BslFI GGGAC 2 cut(s) 126, 835
BsmAI GTCTC 6 cut(s) 98, 185, 831, 913, 1116, 1148
BsmBI CGTCTC 1 cut(s) 831
BsmFI GGGAC 2 cut(s) 126, 835
BsnI GGCC 4 cut(s) 324, 668, 746, 1017
Bso31I GGTCTC 3 cut(s) 98, 185, 913
Bsp120I GGGCCC 1 cut(s) 666
Bsp1286I GDGCHC 4 cut(s) 41, 261, 367, 670
Bsp1407I TGTACA 2 cut(s) 31, 696
Bsp143I GATC 3 cut(s) 56, 136, 1077
Bsp19I CCATGG 3 cut(s) 741, 747, 1023
BspACI CCGC 2 cut(s) 596, 1096
BspANI GGCC 4 cut(s) 324, 668, 746, 1017
BspCNI CTCAG 5 cut(s) 37, 256, 1150, 1163, 1186
BspLI GGNNCC 2 cut(s) 668, 669
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 1 cut(s) 1085
BspTNI GGTCTC 3 cut(s) 98, 185, 913
BsrBI CCGCTC 1 cut(s) 1098
BsrFI RCCGGY 1 cut(s) 107
BsrGI TGTACA 2 cut(s) 31, 696
BsrI ACTGG 2 cut(s) 181, 607
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 4 cut(s) 741, 747, 766, 1023
BssMI GATC 3 cut(s) 56, 136, 1077
BssT1I CCWWGG 3 cut(s) 741, 747, 1023
Bst2UI CCWGG 2 cut(s) 626, 924
Bst4CI ACNGT 2 cut(s) 362, 767
BstAUI TGTACA 2 cut(s) 31, 696
BstC8I GCNNGC 1 cut(s) 466
BstDEI CTNAG 5 cut(s) 24, 264, 1150, 1158, 1194
BstDSI CCRYGG 4 cut(s) 741, 747, 766, 1023
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 7 cut(s) 214, 241, 343, 478, 737, 856, 1204
BstKTI GATC 3 cut(s) 59, 139, 1080
BstMAI GTCTC 6 cut(s) 98, 185, 831, 913, 1116, 1148
BstMBI GATC 3 cut(s) 56, 136, 1077
BstMWI GCNNNNNNNGC 4 cut(s) 195, 595, 743, 852
BstNI CCWGG 2 cut(s) 626, 924
BstNSI RCATGY 2 cut(s) 32, 828
BstSCI CCNGG 6 cut(s) 142, 624, 818, 859, 922, 1012
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 2 cut(s) 367, 670
BstV1I GCAGC 3 cut(s) 520, 667, 990
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 1077
BstXI CCANNNNNNTGG 2 cut(s) 748, 754
BstYI RGATCY 1 cut(s) 1077
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 4 cut(s) 324, 668, 746, 1017
BtgI CCRYGG 4 cut(s) 741, 747, 766, 1023
BtsCI GGATG 7 cut(s) 214, 241, 343, 478, 737, 856, 1204
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 1 cut(s) 466
CaiI CAGNNNCTG 1 cut(s) 1157
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 5 cut(s) 323, 666, 667, 974, 1015
CseI GACGC 2 cut(s) 392, 1191
Csp6I GTAC 3 cut(s) 32, 319, 697
CviQI GTAC 3 cut(s) 32, 319, 697
DdeI CTNAG 5 cut(s) 24, 264, 1150, 1158, 1194
DpnI GATC 3 cut(s) 58, 138, 1079
DpnII GATC 3 cut(s) 56, 136, 1077
EaeI YGGCCR 1 cut(s) 744
Ecl136II GAGCTC 2 cut(s) 39, 259
Eco130I CCWWGG 3 cut(s) 741, 747, 1023
Eco24I GRGCYC 3 cut(s) 41, 261, 670
Eco31I GGTCTC 3 cut(s) 98, 185, 913
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 2 cut(s) 39, 259
Eco57I CTGAAG 2 cut(s) 320, 931
EcoICRI GAGCTC 2 cut(s) 39, 259
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 667
EcoRII CCWGG 2 cut(s) 624, 922
EcoT14I CCWWGG 3 cut(s) 741, 747, 1023
EcoT38I GRGCYC 3 cut(s) 41, 261, 670
ErhI CCWWGG 3 cut(s) 741, 747, 1023
Esp3I CGTCTC 1 cut(s) 831
FaqI GGGAC 2 cut(s) 126, 835
FauNDI CATATG 2 cut(s) 511, 577
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 3 cut(s) 534, 681, 1004
FokI GGATG 7 cut(s) 201, 248, 330, 465, 724, 843, 1211
FriOI GRGCYC 3 cut(s) 41, 261, 670
Fsp4HI GCNGC 3 cut(s) 534, 681, 1004
FspBI CTAG 2 cut(s) 522, 552
GluI GCNGC 3 cut(s) 534, 681, 1004
GsaI CCCAGC 1 cut(s) 667
HaeIII GGCC 4 cut(s) 324, 668, 746, 1017
HapII CCGG 7 cut(s) 108, 144, 281, 726, 819, 861, 1013
HgaI GACGC 2 cut(s) 392, 1191
HinfI GANTC 6 cut(s) 52, 124, 453, 709, 960, 1103
HpaII CCGG 7 cut(s) 108, 144, 281, 726, 819, 861, 1013
HphI GGTGA 6 cut(s) 158, 422, 624, 1047, 1074, 1200
Hpy166II GTNNAC 4 cut(s) 365, 395, 573, 1127
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 4 cut(s) 365, 395, 573, 1127
HpyCH4III ACNGT 2 cut(s) 362, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 595, 743, 852
HpyF3I CTNAG 5 cut(s) 24, 264, 1150, 1158, 1194
Kzo9I GATC 3 cut(s) 56, 136, 1077
LmnI GCTCC 3 cut(s) 256, 264, 308
Lsp1109I GCAGC 3 cut(s) 520, 667, 990
LweI GCATC 4 cut(s) 67, 692, 746, 1189
MaeI CTAG 2 cut(s) 522, 552
MaeIII GTNAC 3 cut(s) 206, 941, 1060
MalI GATC 3 cut(s) 58, 138, 1079
MbiI CCGCTC 1 cut(s) 1098
MboI GATC 3 cut(s) 56, 136, 1077
MboII GAAGA 8 cut(s) 51, 86, 100, 218, 313, 718, 902, 1150
MflI RGATCY 1 cut(s) 1077
MhlI GDGCHC 4 cut(s) 41, 261, 367, 670
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 4 cut(s) 161, 270, 470, 986
MluNI TGGCCA 1 cut(s) 746
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 109, 247, 492, 600, 646, 663, 666, 1068, 1189
Mox20I TGGCCA 1 cut(s) 746
MscI TGGCCA 1 cut(s) 746
MseI TTAA 2 cut(s) 170, 560
MslI CAYNNNNRTG 3 cut(s) 746, 752, 1210
Msp20I TGGCCA 1 cut(s) 746
MspI CCGG 7 cut(s) 108, 144, 281, 726, 819, 861, 1013
MspR9I CCNGG 6 cut(s) 144, 626, 820, 861, 924, 1014
MvaI CCWGG 2 cut(s) 626, 924
MwoI GCNNNNNNNGC 4 cut(s) 195, 595, 743, 852
NciI CCSGG 4 cut(s) 144, 820, 861, 1014
NcoI CCATGG 3 cut(s) 741, 747, 1023
NdeI CATATG 2 cut(s) 511, 577
NdeII GATC 3 cut(s) 56, 136, 1077
NlaIV GGNNCC 2 cut(s) 668, 669
NspI RCATGY 2 cut(s) 32, 828
PciI ACATGT 1 cut(s) 824
PfeI GAWTC 6 cut(s) 52, 124, 453, 709, 960, 1103
PflMI CCANNNNNTGG 2 cut(s) 938, 1172
PfoI TCCNGGA 1 cut(s) 859
PkrI GCNGC 3 cut(s) 535, 682, 1005
PscI ACATGT 1 cut(s) 824
PsiI TTATAA 1 cut(s) 896
Psp124BI GAGCTC 2 cut(s) 41, 261
Psp6I CCWGG 2 cut(s) 624, 922
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 2 cut(s) 624, 922
PspN4I GGNNCC 2 cut(s) 668, 669
PspOMI GGGCCC 1 cut(s) 666
PspPI GGNCC 5 cut(s) 323, 666, 667, 974, 1015
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 1 cut(s) 1077
RsaI GTAC 3 cut(s) 33, 320, 698
RsaNI GTAC 3 cut(s) 32, 319, 697
RseI CAYNNNNRTG 3 cut(s) 746, 752, 1210
SacI GAGCTC 2 cut(s) 41, 261
SaqAI TTAA 2 cut(s) 170, 560
SatI GCNGC 3 cut(s) 534, 681, 1004
Sau3AI GATC 3 cut(s) 56, 136, 1077
Sau96I GGNCC 5 cut(s) 323, 666, 667, 974, 1015
ScrFI CCNGG 6 cut(s) 144, 626, 820, 861, 924, 1014
SduI GDGCHC 4 cut(s) 41, 261, 367, 670
SfaNI GCATC 4 cut(s) 67, 692, 746, 1189
SfcI CTRYAG 1 cut(s) 534
SinI GGWCC 1 cut(s) 974
SmiMI CAYNNNNRTG 3 cut(s) 746, 752, 1210
SmlI CTYRAG 2 cut(s) 639, 657
SmoI CTYRAG 2 cut(s) 639, 657
Sse9I AATT 4 cut(s) 161, 270, 470, 986
SsiI CCGC 2 cut(s) 596, 1096
SspMI CTAG 2 cut(s) 522, 552
SstI GAGCTC 2 cut(s) 41, 261
StyD4I CCNGG 6 cut(s) 142, 624, 818, 859, 922, 1012
StyI CCWWGG 3 cut(s) 741, 747, 1023
TaaI ACNGT 2 cut(s) 362, 767
TaqI TCGA 4 cut(s) 50, 55, 72, 990
TasI AATT 4 cut(s) 161, 270, 470, 986
TatI WGTACW 2 cut(s) 31, 696
TfiI GAWTC 6 cut(s) 52, 124, 453, 709, 960, 1103
Tru1I TTAA 2 cut(s) 170, 560
Tru9I TTAA 2 cut(s) 170, 560
TseI GCWGC 3 cut(s) 533, 680, 1003
TspDTI ATGAA 2 cut(s) 107, 827
TspGWI ACGGA 2 cut(s) 117, 849
Van91I CCANNNNNTGG 2 cut(s) 938, 1172
VneI GTGCAC 1 cut(s) 363
VpaK11BI GGWCC 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 2 cut(s) 470, 986
XceI RCATGY 2 cut(s) 32, 828
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 2 cut(s) 522, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.