pycom17g09960

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002970
Physical Location & Seq
Forward (+)
7668858 .. 7670228
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09960.3

Sequence Viewer

Length: 666 bp
ATGCCTGTTGTTCTTCCGCCCCAAGGCATTGTGCCTGCCCATGGATGGAACACCTATGTGGGAAACATGAGCCCTGTGTCTTCCACTGGTATTCTTGGATCGAACCTCACATACAACTCTAGAACTCAGGGAGAGTCAGCTTCCAACGGGCAGGTTCATTTGTCAAGTCCAAGTCTCCCACAGAGGCCTGATCAGCCTGAATGCCGGTACTTTATGAATACTGGGACGTGCAAATATGGAATAGATTGCAAATACCATCACCCCAAAGAAAGGATCGCAGAATCTGCTGCAAGCCCACTTGGGCTTCCTTCGAGACCTGGGCAACCTGCATGCTCATACTTCATAATGTATGGAATCTGCAAGTATGGACCGACTTGCAAATTTGATCATCCCTACATGGAACAATCTGCTAGCTATAATTTCGGTATGCAGGCGATGCCAGTGCTTGATTCATCTCTCCTAAGTTATCCAAGAGGCTGGCCAGTGGCTCATGGACTAGAGACATCCCCTTCCATACCATCAAAATCTCCTGATGGGGCCAAAAAACCAGTGAGCAACAACTACCAAAACTCCAATACAAAGATTTCAGAAGATTCCCCGGAACAAGTGGGTTCTCTGCCAGCTTCTCCATCCTCCCCAGAACCTGTACAAGAACAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

222

Amino Acids

23.8

Weight (kDa)

6.58

Isoelectric Point (pI)

72.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 142, 334
AciI CCGC 1 cut(s) 17
AclWI GGATC 2 cut(s) 106, 281
AcoI YGGCCR 1 cut(s) 479
AcsI RAATTY 1 cut(s) 380
AfaI GTAC 2 cut(s) 209, 648
AfiI CCNNNNNNNGG 4 cut(s) 23, 41, 45, 270
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 1 cut(s) 316
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 3 cut(s) 140, 414, 623
AluI AGCT 3 cut(s) 140, 414, 623
Alw26I GTCTC 3 cut(s) 179, 307, 494
AlwI GGATC 2 cut(s) 106, 281
AlwNI CAGNNNCTG 2 cut(s) 284, 644
AoxI GGCC 3 cut(s) 185, 479, 537
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 368, 537
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 251
AsuNHI GCTAGC 1 cut(s) 410
AvaII GGWCC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 481
BanII GRGCYC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 274
BccI CCATC 5 cut(s) 39, 264, 526, 527, 637
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 2 cut(s) 190, 385
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 3 cut(s) 179, 307, 494
BfaI CTAG 3 cut(s) 120, 411, 497
BfuAI ACCTGC 2 cut(s) 142, 334
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 2 cut(s) 318, 599
Bme18I GGWCC 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 2 cut(s) 368, 537
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 2 cut(s) 318, 599
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 426
BmtI GCTAGC 1 cut(s) 414
BmuI ACTGGG 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 599
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 4 cut(s) 22, 40, 317, 597
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 41, 45, 270
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 5 cut(s) 91, 226, 440, 482, 548
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 4 cut(s) 22, 40, 317, 597
BseGI GGATG 4 cut(s) 50, 388, 503, 629
BseLI CCNNNNNNNGG 4 cut(s) 23, 41, 45, 270
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 5 cut(s) 91, 226, 440, 482, 548
BseXI GCAGC 1 cut(s) 274
BshFI GGCC 3 cut(s) 187, 481, 539
BsiSI CCGG 2 cut(s) 205, 599
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 4 cut(s) 23, 41, 45, 270
BsmAI GTCTC 3 cut(s) 179, 307, 494
BsmFI GGGAC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 3 cut(s) 187, 481, 539
Bso31I GGTCTC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 4 cut(s) 98, 190, 273, 385
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 1 cut(s) 17
BspANI GGCC 3 cut(s) 187, 481, 539
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 538
BspMI ACCTGC 2 cut(s) 142, 334
BspOI GCTAGC 1 cut(s) 414
BspPI GGATC 2 cut(s) 106, 281
BspTNI GGTCTC 1 cut(s) 307
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 5 cut(s) 91, 226, 440, 482, 548
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 4 cut(s) 22, 40, 317, 597
BssMI GATC 4 cut(s) 98, 190, 273, 385
BssT1I CCWWGG 2 cut(s) 22, 40
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 657
BstAPI GCANNNNNTGC 2 cut(s) 284, 436
BstAUI TGTACA 1 cut(s) 646
BstC8I GCNNGC 7 cut(s) 36, 292, 331, 412, 432, 479, 621
BstDEI CTNAG 2 cut(s) 126, 461
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 50, 388, 503, 629
BstKTI GATC 4 cut(s) 101, 193, 276, 388
BstMAI GTCTC 3 cut(s) 179, 307, 494
BstMBI GATC 4 cut(s) 98, 190, 273, 385
BstMWI GCNNNNNNNGC 3 cut(s) 193, 284, 436
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 316, 597
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 72
BstXI CCANNNNNNTGG 1 cut(s) 477
BsuRI GGCC 3 cut(s) 187, 481, 539
BtgI CCRYGG 1 cut(s) 40
BtgZI GCGATG 1 cut(s) 449
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 4 cut(s) 50, 388, 503, 629
BtsIMutI CAGTG 4 cut(s) 84, 447, 489, 555
BveI ACCTGC 2 cut(s) 142, 334
Cac8I GCNNGC 7 cut(s) 36, 292, 331, 412, 432, 479, 621
CaiI CAGNNNCTG 2 cut(s) 284, 644
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 368, 537
Csp6I GTAC 2 cut(s) 208, 647
CviAII CATG 5 cut(s) 41, 67, 330, 397, 491
CviQI GTAC 2 cut(s) 208, 647
DdeI CTNAG 2 cut(s) 126, 461
DpnI GATC 4 cut(s) 100, 192, 275, 387
DpnII GATC 4 cut(s) 98, 190, 273, 385
EaeI YGGCCR 1 cut(s) 479
EciI GGCGGA 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 22, 40
Eco147I AGGCCT 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 22, 40
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 22, 40
FaeI CATG 5 cut(s) 44, 70, 333, 400, 494
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 40, 66, 329, 396, 490
FbaI TGATCA 2 cut(s) 190, 385
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 4 cut(s) 57, 375, 490, 616
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 3 cut(s) 120, 411, 497
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 3 cut(s) 187, 481, 539
HapII CCGG 2 cut(s) 205, 599
Hin1II CATG 5 cut(s) 44, 70, 333, 400, 494
HinfI GANTC 5 cut(s) 134, 281, 354, 449, 593
HpaII CCGG 2 cut(s) 205, 599
HphI GGTGA 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 589
Hpy188III TCNNGA 3 cut(s) 120, 312, 530
HpyAV CCTTC 2 cut(s) 318, 519
HpyCH4III ACNGT 1 cut(s) 657
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 7 cut(s) 231, 249, 290, 329, 360, 378, 430
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 284, 436
HpyF3I CTNAG 2 cut(s) 126, 461
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 5 cut(s) 44, 70, 333, 400, 494
Ksp22I TGATCA 2 cut(s) 190, 385
Kzo9I GATC 4 cut(s) 98, 190, 273, 385
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 426
MaeI CTAG 3 cut(s) 120, 411, 497
MaeII ACGT 1 cut(s) 227
MalI GATC 4 cut(s) 100, 192, 275, 387
MboI GATC 4 cut(s) 98, 190, 273, 385
MboII GAAGA 3 cut(s) 5, 72, 602
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 2 cut(s) 380, 418
MluNI TGGCCA 1 cut(s) 481
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 4 cut(s) 116, 177, 467, 643
Mox20I TGGCCA 1 cut(s) 481
MscI TGGCCA 1 cut(s) 481
MseI TTAA 1 cut(s) 664
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 481
MspI CCGG 2 cut(s) 205, 599
MspR9I CCNGG 2 cut(s) 318, 599
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 3 cut(s) 193, 284, 436
NciI CCSGG 1 cut(s) 599
NcoI CCATGG 1 cut(s) 40
NdeII GATC 4 cut(s) 98, 190, 273, 385
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 5 cut(s) 44, 70, 333, 400, 494
NlaIV GGNNCC 1 cut(s) 538
NspI RCATGY 1 cut(s) 333
OliI CACNNNNGTG 1 cut(s) 56
PaeI GCATGC 1 cut(s) 333
PceI AGGCCT 1 cut(s) 187
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 4 cut(s) 281, 354, 449, 593
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 2 cut(s) 368, 537
PsrI GAACNNNNNNTAC 2 cut(s) 95, 127
PstNI CAGNNNCTG 2 cut(s) 284, 644
RsaI GTAC 2 cut(s) 209, 648
RsaNI GTAC 2 cut(s) 208, 647
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 1 cut(s) 664
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 4 cut(s) 98, 190, 273, 385
Sau96I GGNCC 2 cut(s) 368, 537
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 318, 599
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 1 cut(s) 426
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 56
SphI GCATGC 1 cut(s) 333
Sse9I AATT 2 cut(s) 380, 418
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 1 cut(s) 17
SspMI CTAG 3 cut(s) 120, 411, 497
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 316, 597
StyI CCWWGG 2 cut(s) 22, 40
TaaI ACNGT 1 cut(s) 657
TaiI ACGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 101, 311
TaqII GACCGA 1 cut(s) 385
TasI AATT 2 cut(s) 380, 418
TatI WGTACW 1 cut(s) 646
TfiI GAWTC 4 cut(s) 281, 354, 449, 593
Tru1I TTAA 1 cut(s) 664
Tru9I TTAA 1 cut(s) 664
TscAI CASTG 4 cut(s) 91, 447, 489, 555
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 4 cut(s) 146, 230, 331, 441
TspRI CASTG 4 cut(s) 91, 447, 489, 555
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 380
XbaI TCTAGA 1 cut(s) 119
XceI RCATGY 1 cut(s) 333
XspI CTAG 3 cut(s) 120, 411, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.