MD03G1279200.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002970
Physical Location & Seq
Reverse (-)
36091920 .. 36094204
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1279200.v1.1.491

Sequence Viewer

Length: 936 bp
ATGTTTAATGTTACATGTCGTGCCTTTTTGTGCCCCTATGTTGATTTTATTTTTTTTGTTCAAAACCCCTATGTCGATTATTACGAGCATTACCCCATATATATGGCGAATTGCGGCGTGCAACCGTGTACTACATGCAAATTTGGTCAGTGCAACTTTGGATCTACATGCAAATTTGACCATCCAGTGCAGACCATGAGGTACAACCTCTCAGCTTCATCTCTCGTTGATATGCCTGTTCAAATGTGGCAATTGGGGCTGACGAGCAGCGAGTCATACCCGGAGAGACCTGGCGTGCCCAACTGTGTCTACTACATGCGAACCGGGTTCTGTGGATACGGTGGTCGGTGCCGGTACAACCATCCTCGTGATCGTGCTGCGGTCGTGGCAGTTGTAAGAGCTACAGGGGATTACCCAGAGAGAGTGGGGGAACCTATATGTCAGTATTATTTGAAGACTGGGACCTGTAAATTTGGTGCGTCCTGCAAGTTCCACCATCCAAAACATGGATACCCATTACGACCGGGCGAGAATGAATGCTCCTACTATTTGAAAACGGGGCAATACAAATTTGGCATAACCTGTAAATTCCATCACCCACAGCCTGCTGGAACAACAATACCGGCTTCTGCACCCCAATTTTATCCGTCGTTGTTCCGATTCCGGGTTGGAGTCCGTATTCGTCCTGTACTATCTCCTGGAGCTCAACCCACAGTTGGAGCAACTTCTTTATATGGAATAACACAGCTATCTTCTCCAACACACGGACTTGCAAGCCCTTATACCTCCATAACCTCTTCTGTTGGTCCTTCTAGCAGCAACCCGAGTGGACAAGTTTTCCCAGAGAGGCCTGGCGAACTTGAATGCCAGTATTATCTGAAAACTGGGGACTGTAAACATGGATCATCTTGTAGGTATCATCATCCTCGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

312

Amino Acids

34.82

Weight (kDa)

8.86

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 98 - 123 2.6e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 144 - 168 8.6e-11 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 286 - 310 1.9e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 506
AccI GTMKAC 1 cut(s) 309
AciI CCGC 2 cut(s) 114, 380
AclWI GGATC 2 cut(s) 169, 910
AcsI RAATTY 5 cut(s) 140, 173, 470, 569, 587
AfaI GTAC 4 cut(s) 130, 203, 356, 690
AfiI CCNNNNNNNGG 4 cut(s) 506, 664, 716, 764
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 5 cut(s) 62, 242, 454, 553, 863
AjnI CCWGG 3 cut(s) 289, 697, 850
AluBI AGCT 4 cut(s) 215, 401, 704, 748
AluI AGCT 4 cut(s) 215, 401, 704, 748
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 169, 910
Ama87I CYCGRG 2 cut(s) 823, 927
AoxI GGCC 1 cut(s) 848
ApeKI GCWGC 3 cut(s) 267, 377, 816
ApoI RAATTY 5 cut(s) 140, 173, 470, 569, 587
AspS9I GGNCC 2 cut(s) 462, 806
AsuC2I CCSGG 4 cut(s) 281, 325, 525, 665
AsuHPI GGTGA 1 cut(s) 587
AvaI CYCGRG 2 cut(s) 823, 927
AvaII GGWCC 2 cut(s) 462, 806
BaeGI GKGCMC 2 cut(s) 35, 300
BaeI ACNNNNGTAYC 2 cut(s) 328, 361
BanI GGYRCC 1 cut(s) 348
BanII GRGCYC 1 cut(s) 706
BauI CACGAG 1 cut(s) 366
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 3 cut(s) 279, 364, 828
BccI CCATC 4 cut(s) 189, 369, 504, 600
BciT130I CCWGG 3 cut(s) 291, 699, 852
BciVI GTATCC 2 cut(s) 329, 503
BcnI CCSGG 4 cut(s) 281, 325, 525, 665
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 1 cut(s) 402
BfuI GTATCC 2 cut(s) 329, 503
BisI GCNGC 4 cut(s) 115, 268, 378, 817
BlsI GCNGC 4 cut(s) 116, 269, 379, 818
Bme1390I CCNGG 7 cut(s) 281, 291, 325, 525, 665, 699, 852
Bme18I GGWCC 2 cut(s) 462, 806
BmeT110I CYCGRG 2 cut(s) 823, 927
BmgT120I GGNCC 2 cut(s) 462, 806
BmiI GGNNCC 3 cut(s) 350, 432, 463
BmrFI CCNGG 7 cut(s) 281, 291, 325, 525, 665, 699, 852
BmrI ACTGGG 2 cut(s) 468, 894
BmuI ACTGGG 2 cut(s) 468, 894
BpiI GAAGAC 1 cut(s) 461
BpmI CTGGAG 1 cut(s) 720
BpuMI CCSGG 4 cut(s) 281, 325, 525, 665
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 926
Bsc4I CCNNNNNNNGG 4 cut(s) 506, 664, 716, 764
Bse118I RCCGGY 2 cut(s) 351, 622
Bse1I ACTGG 4 cut(s) 185, 463, 868, 889
BseBI CCWGG 3 cut(s) 291, 699, 852
BseDI CCNNGG 1 cut(s) 926
BseGI GGATG 4 cut(s) 181, 361, 496, 922
BseLI CCNNNNNNNGG 4 cut(s) 506, 664, 716, 764
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 4 cut(s) 185, 463, 868, 889
BseSI GKGCMC 2 cut(s) 35, 300
BseXI GCAGC 3 cut(s) 279, 364, 828
BsgI GTGCAG 2 cut(s) 209, 615
Bsh1285I CGRYCG 2 cut(s) 384, 524
BshFI GGCC 1 cut(s) 850
BshNI GGYRCC 1 cut(s) 348
BsiEI CGRYCG 2 cut(s) 384, 524
BsiHKAI GWGCWC 1 cut(s) 706
BsiHKCI CYCGRG 2 cut(s) 823, 927
BsiSI CCGG 6 cut(s) 281, 324, 352, 524, 623, 664
BslFI GGGAC 2 cut(s) 475, 902
BslI CCNNNNNNNGG 4 cut(s) 506, 664, 716, 764
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 475, 902
BsmI GAATGC 2 cut(s) 542, 869
BsnI GGCC 1 cut(s) 850
Bso31I GGTCTC 1 cut(s) 280
BsoBI CYCGRG 2 cut(s) 823, 927
Bsp1286I GDGCHC 3 cut(s) 35, 300, 706
Bsp143I GATC 3 cut(s) 161, 370, 902
BspACI CCGC 2 cut(s) 114, 380
BspANI GGCC 1 cut(s) 850
BspCNI CTCAG 1 cut(s) 224
BspLI GGNNCC 3 cut(s) 350, 432, 463
BspPI GGATC 2 cut(s) 169, 910
BspT107I GGYRCC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 280
BsrFI RCCGGY 2 cut(s) 351, 622
BsrI ACTGG 4 cut(s) 185, 463, 868, 889
BssAI RCCGGY 2 cut(s) 351, 622
BssECI CCNNGG 1 cut(s) 926
BssMI GATC 3 cut(s) 161, 370, 902
BssSI CACGAG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 291, 699, 852
Bst4CI ACNGT 5 cut(s) 126, 305, 341, 715, 893
Bst6I CTCTTC 1 cut(s) 802
BstC8I GCNNGC 4 cut(s) 119, 296, 606, 775
BstDEI CTNAG 1 cut(s) 211
BstF5I GGATG 4 cut(s) 181, 361, 496, 922
BstKTI GATC 3 cut(s) 164, 373, 905
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 161, 370, 902
BstMCI CGRYCG 2 cut(s) 384, 524
BstMWI GCNNNNNNNGC 2 cut(s) 256, 386
BstNI CCWGG 3 cut(s) 291, 699, 852
BstNSI RCATGY 4 cut(s) 18, 138, 171, 319
BstSCI CCNGG 7 cut(s) 279, 289, 323, 523, 663, 697, 850
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 2 cut(s) 35, 300
BstV1I GCAGC 3 cut(s) 279, 364, 828
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 103
BstYI RGATCY 1 cut(s) 161
BsuI GTATCC 2 cut(s) 329, 503
BsuRI GGCC 1 cut(s) 850
BtsCI GGATG 4 cut(s) 181, 361, 496, 922
BtsIMutI CAGTG 2 cut(s) 155, 192
Cac8I GCNNGC 4 cut(s) 119, 296, 606, 775
Cfr10I RCCGGY 2 cut(s) 351, 622
Cfr13I GGNCC 2 cut(s) 462, 806
CseI GACGC 1 cut(s) 468
Csp6I GTAC 4 cut(s) 129, 202, 355, 689
CspCI CAANNNNNGTGG 2 cut(s) 808, 843
CviAII CATG 7 cut(s) 15, 135, 168, 196, 316, 506, 899
CviJI RGCY 9 cut(s) 215, 259, 401, 604, 626, 704, 748, 777, 850
CviKI_1 RGCY 9 cut(s) 215, 259, 401, 604, 626, 704, 748, 777, 850
CviQI GTAC 4 cut(s) 129, 202, 355, 689
DdeI CTNAG 1 cut(s) 211
DpnI GATC 3 cut(s) 163, 372, 904
DpnII GATC 3 cut(s) 161, 370, 902
Eam1104I CTCTTC 1 cut(s) 802
EarI CTCTTC 1 cut(s) 802
Ecl136II GAGCTC 1 cut(s) 704
Eco147I AGGCCT 1 cut(s) 850
Eco24I GRGCYC 1 cut(s) 706
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 2 cut(s) 462, 806
Eco53kI GAGCTC 1 cut(s) 704
Eco88I CYCGRG 2 cut(s) 823, 927
EcoICRI GAGCTC 1 cut(s) 704
EcoO109I RGGNCCY 1 cut(s) 462
EcoRII CCWGG 3 cut(s) 289, 697, 850
EcoT38I GRGCYC 1 cut(s) 706
FaeI CATG 7 cut(s) 18, 138, 171, 199, 319, 509, 902
FaqI GGGAC 2 cut(s) 475, 902
FatI CATG 7 cut(s) 14, 134, 167, 195, 315, 505, 898
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 115, 268, 378, 817
FokI GGATG 4 cut(s) 168, 348, 483, 909
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 4 cut(s) 115, 268, 378, 817
FspBI CTAG 1 cut(s) 813
GluI GCNGC 4 cut(s) 115, 268, 378, 817
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 1 cut(s) 850
HapII CCGG 6 cut(s) 281, 324, 352, 524, 623, 664
HgaI GACGC 1 cut(s) 468
Hin1II CATG 7 cut(s) 18, 138, 171, 199, 319, 509, 902
HinfI GANTC 3 cut(s) 272, 660, 672
HpaII CCGG 6 cut(s) 281, 324, 352, 524, 623, 664
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 4 cut(s) 129, 310, 830, 896
Hpy188I TCNGA 2 cut(s) 659, 879
Hpy188III TCNNGA 2 cut(s) 368, 929
Hpy8I GTNNAC 4 cut(s) 129, 310, 830, 896
Hpy99I CGWCG 1 cut(s) 652
HpyAV CCTTC 1 cut(s) 819
HpyCH4III ACNGT 5 cut(s) 126, 305, 341, 715, 893
HpyCH4V TGCA 8 cut(s) 121, 138, 153, 171, 190, 486, 632, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 386
HpyF3I CTNAG 1 cut(s) 211
Hsp92II CATG 7 cut(s) 18, 138, 171, 199, 319, 509, 902
Kzo9I GATC 3 cut(s) 161, 370, 902
LmnI GCTCC 3 cut(s) 545, 701, 719
Lsp1109I GCAGC 3 cut(s) 279, 364, 828
MaeI CTAG 1 cut(s) 813
MaeIII GTNAC 1 cut(s) 10
MalI GATC 3 cut(s) 163, 372, 904
MboI GATC 3 cut(s) 161, 370, 902
MboII GAAGA 3 cut(s) 466, 744, 789
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 3 cut(s) 35, 300, 706
MluCI AATT 8 cut(s) 109, 140, 173, 251, 470, 569, 587, 638
MlyI GAGTC 2 cut(s) 281, 681
MmeI TCCRAC 3 cut(s) 649, 697, 782
MnlI CCTC 7 cut(s) 192, 218, 375, 796, 805, 840, 936
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 2 cut(s) 101, 366
MspI CCGG 6 cut(s) 281, 324, 352, 524, 623, 664
MspR9I CCNGG 7 cut(s) 281, 291, 325, 525, 665, 699, 852
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 2 cut(s) 542, 869
MvaI CCWGG 3 cut(s) 291, 699, 852
MwoI GCNNNNNNNGC 2 cut(s) 256, 386
NciI CCSGG 4 cut(s) 281, 325, 525, 665
NdeII GATC 3 cut(s) 161, 370, 902
NlaIII CATG 7 cut(s) 18, 138, 171, 199, 319, 509, 902
NlaIV GGNNCC 3 cut(s) 350, 432, 463
NspI RCATGY 4 cut(s) 18, 138, 171, 319
PceI AGGCCT 1 cut(s) 850
PciI ACATGT 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 2 cut(s) 542, 869
PfeI GAWTC 1 cut(s) 660
PflMI CCANNNNNTGG 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 697
PkrI GCNGC 4 cut(s) 116, 269, 379, 818
PleI GAGTC 2 cut(s) 280, 680
PpsI GAGTC 2 cut(s) 280, 680
PpuMI RGGWCCY 1 cut(s) 462
PscI ACATGT 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 706
Psp5II RGGWCCY 1 cut(s) 462
Psp6I CCWGG 3 cut(s) 289, 697, 850
PspGI CCWGG 3 cut(s) 289, 697, 850
PspN4I GGNNCC 3 cut(s) 350, 432, 463
PspPI GGNCC 2 cut(s) 462, 806
PspPPI RGGWCCY 1 cut(s) 462
PsuI RGATCY 1 cut(s) 161
RsaI GTAC 4 cut(s) 130, 203, 356, 690
RsaNI GTAC 4 cut(s) 129, 202, 355, 689
RseI CAYNNNNRTG 2 cut(s) 101, 366
SacI GAGCTC 1 cut(s) 706
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 4 cut(s) 115, 268, 378, 817
Sau3AI GATC 3 cut(s) 161, 370, 902
Sau96I GGNCC 2 cut(s) 462, 806
SchI GAGTC 2 cut(s) 281, 681
ScrFI CCNGG 7 cut(s) 281, 291, 325, 525, 665, 699, 852
SduI GDGCHC 3 cut(s) 35, 300, 706
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 2 cut(s) 462, 806
SmiMI CAYNNNNRTG 2 cut(s) 101, 366
Sse9I AATT 8 cut(s) 109, 140, 173, 251, 470, 569, 587, 638
SseBI AGGCCT 1 cut(s) 850
SsiI CCGC 2 cut(s) 114, 380
SspMI CTAG 1 cut(s) 813
SstI GAGCTC 1 cut(s) 706
StuI AGGCCT 1 cut(s) 850
StyD4I CCNGG 7 cut(s) 279, 289, 323, 523, 663, 697, 850
TaaI ACNGT 5 cut(s) 126, 305, 341, 715, 893
TaqI TCGA 1 cut(s) 75
TasI AATT 8 cut(s) 109, 140, 173, 251, 470, 569, 587, 638
TatI WGTACW 2 cut(s) 128, 688
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 2 cut(s) 155, 192
TseI GCWGC 3 cut(s) 267, 377, 816
TspDTI ATGAA 2 cut(s) 207, 549
TspGWI ACGGA 3 cut(s) 636, 665, 780
TspRI CASTG 2 cut(s) 155, 192
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 462, 806
XapI RAATTY 5 cut(s) 140, 173, 470, 569, 587
XceI RCATGY 4 cut(s) 18, 138, 171, 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.