AT2G32930

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002970
Physical Location & Seq
Reverse (-)
13965714 .. 13968957
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32930.1

Sequence Viewer

Length: 1362 bp
ATGTCGGAAACTCAGCAGCAGGTTCAGAACTCTACTGGGTCGATTCGATCTCCCGACAAAATTGAAGATACCTTCAGGAGGATGAAAGTTAATGAAGACAATATGGAGCAATCTAGTCCATATCCTGATCGTCCTGGTGAGCGAGATTGCCAGTTCTTTCTGAGAACTGGTCAGTGTGGCTATGGAAACTCTTGTCGATACAATCATCCTCTTACTAATCTTCCACAGGGTATTATTTATTACAGAGACCAATTGCCTGAGAGAGTTGGACAGCCTGACTGTGAGACAGGAGCCTGTAAGTATGGCCCAACATGTAAATATCACCACCCTAAAGACAGGAATGGTGCTGGACCCGTGCTGTTCAATGTTCTCGGTTTGCCTATGCGGCAGGGTGAGAAGCCATGTCCATATTACATGCAGACAGGATTGTGTCGATTCGGAGTTGCCTGCAAATTCCATCATCCTCATCCTCATTCTCAGCCTTCTAATGGCCACTCTGCATATGCAATGTCCAGCTTTCCGTCTGTTGGTTTTCCTTATGCCAGTGGAATGACAATGGTGTCTTTGCCTCCTGCAACATATGGAGCTATTCCACGTCCTCAAGTTCCTCAGTCTCAGGCCTATATGCCCTACATGGTTGCACCCTCCCAAGGCCTTTTACCTCCTCAAGGCTGGGCCACTTACATGACTGCATCTAACCCCATTTACAATATGAAAACTCAACTCGACTCCAGTTCTAGCGCATCGGTGGCTGTGACCGTGACATCACATCATCACAGTTTTTCTGAAAGAGCTGAATGTAGGTTTTTTATGAACACTGGAACTTGTAAATATGGTGATGATTGCAAATACAGTCATCCGAAAGAAAGATTGTTACAATCACCACCAACTCTCTTGAACCCTATTGTTCTTCCAGCAAGACCAGGACAGCCAGCATGTGGTAACTTCAAGGCCTACGGATTTTGCAAGTTTGGAGCAAACTGCAAATTTGATCACTCAATGCTGCTAAACCCTTATAACAATACGGGCTTGGCCATGTCTTCTCTGCCTACTCCTTATCCTTATGCTCCACCTGTTTCAACTAATTTGAGGATCTCATCGCCACCAAGCCCCTCTGATATGACTACCCTATCCAATGGTAAACCCGCTGCTGCAGAAGCTCAAAGCTTGGAGACTGAGAAACAAGACGATAGTCCAACAGAACCAGAAAAATCAGAAGTGGAGGACTCATTGCCACCAAACGGCTCTGATTCAACCTCCCTGCCCAATGATAAACCTGATGCAGAGACTGAGAAACAAGACGATGATTCTGCAGAACTGGACAGCTCAAAAGTGCAGGATTCATCTGATAAATCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

453

Amino Acids

49.75

Weight (kDa)

6.01

Isoelectric Point (pI)

60.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 45 - 70 3.6e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 131 - 155 3.4e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 264 - 288 2.3e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf_CCCH_4 PF18345 267 - 286 6.2e-08 Zinc finger domain
zf-CCCH PF00642 309 - 334 1.1e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
AasI GACNNNNNNGTC 1 cut(s) 559
Acc36I ACCTGC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 938
AciI CCGC 2 cut(s) 385, 1146
AclWI GGATC 1 cut(s) 1100
AcoI YGGCCR 2 cut(s) 490, 1032
AcsI RAATTY 2 cut(s) 452, 986
AcuI CTGAAG 1 cut(s) 58
AfiI CCNNNNNNNGG 7 cut(s) 78, 488, 527, 650, 668, 938, 1241
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 6 cut(s) 65, 364, 898, 949, 1080, 1254
AjiI CACGTC 1 cut(s) 596
AjnI CCWGG 2 cut(s) 133, 922
AluBI AGCT 6 cut(s) 516, 587, 794, 1160, 1167, 1326
AluI AGCT 6 cut(s) 516, 587, 794, 1160, 1167, 1326
Alw26I GTCTC 5 cut(s) 240, 278, 618, 1166, 1280
AlwI GGATC 1 cut(s) 1100
AlwNI CAGNNNCTG 1 cut(s) 1289
AoxI GGCC 7 cut(s) 304, 490, 618, 652, 675, 951, 1032
ApeKI GCWGC 4 cut(s) 16, 1003, 1148, 1151
ApoI RAATTY 2 cut(s) 452, 986
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 3 cut(s) 305, 350, 675
AsuHPI GGTGA 5 cut(s) 149, 314, 404, 848, 873
AvaII GGWCC 1 cut(s) 350
BalI TGGCCA 2 cut(s) 492, 1034
BbsI GAAGAC 2 cut(s) 102, 1032
BbvI GCAGC 4 cut(s) 28, 990, 1135, 1138
BccI CCATC 1 cut(s) 465
BceAI ACGGC 1 cut(s) 1258
BcgI CGANNNNNNTGC 2 cut(s) 1292, 1326
BciT130I CCWGG 2 cut(s) 135, 924
BclI TGATCA 1 cut(s) 991
BcoDI GTCTC 5 cut(s) 240, 278, 618, 1166, 1280
BfaI CTAG 2 cut(s) 114, 738
BfmI CTRYAG 2 cut(s) 1152, 1311
BfuAI ACCTGC 1 cut(s) 10
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 5 cut(s) 17, 386, 1004, 1149, 1152
BlsI GCNGC 5 cut(s) 18, 387, 1005, 1150, 1153
Bme1390I CCNGG 2 cut(s) 135, 924
Bme18I GGWCC 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 596
BmgT120I GGNCC 3 cut(s) 305, 350, 675
BmiI GGNNCC 2 cut(s) 292, 352
BmrFI CCNGG 2 cut(s) 135, 924
BmrI ACTGGG 1 cut(s) 45
BmsI GCATC 3 cut(s) 701, 752, 1270
BmuI ACTGGG 1 cut(s) 45
BoxI GACNNNNGTC 1 cut(s) 1191
BpiI GAAGAC 2 cut(s) 102, 1032
BpmI CTGGAG 1 cut(s) 715
BpuEI CTTGAG 2 cut(s) 585, 651
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 649
Bsc4I CCNNNNNNNGG 7 cut(s) 78, 488, 527, 650, 668, 938, 1241
Bse1I ACTGG 7 cut(s) 40, 151, 172, 543, 732, 823, 1323
Bse3DI GCAATG 2 cut(s) 513, 1229
BseBI CCWGG 2 cut(s) 135, 924
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 5 cut(s) 87, 205, 460, 466, 856
BseLI CCNNNNNNNGG 7 cut(s) 78, 488, 527, 650, 668, 938, 1241
BseMI GCAATG 2 cut(s) 513, 1229
BseMII CTCAG 8 cut(s) 26, 152, 249, 491, 623, 629, 1167, 1281
BseNI ACTGG 7 cut(s) 40, 151, 172, 543, 732, 823, 1323
BseRI GAGGAG 1 cut(s) 654
BseXI GCAGC 4 cut(s) 28, 990, 1135, 1138
BseYI CCCAGC 1 cut(s) 672
BsgI GTGCAG 1 cut(s) 1355
BshFI GGCC 7 cut(s) 306, 492, 620, 654, 677, 953, 1034
BslI CCNNNNNNNGG 7 cut(s) 78, 488, 527, 650, 668, 938, 1241
BsmAI GTCTC 5 cut(s) 240, 278, 618, 1166, 1280
BsnI GGCC 7 cut(s) 306, 492, 620, 654, 677, 953, 1034
Bso31I GGTCTC 1 cut(s) 240
Bsp143I GATC 4 cut(s) 47, 127, 991, 1092
BspACI CCGC 2 cut(s) 385, 1146
BspANI GGCC 7 cut(s) 306, 492, 620, 654, 677, 953, 1034
BspCNI CTCAG 8 cut(s) 25, 153, 250, 490, 622, 628, 1168, 1282
BspLI GGNNCC 2 cut(s) 292, 352
BspMAI CTGCAG 2 cut(s) 1156, 1315
BspMI ACCTGC 1 cut(s) 10
BspPI GGATC 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 240
BsrDI GCAATG 2 cut(s) 513, 1229
BsrI ACTGG 7 cut(s) 40, 151, 172, 543, 732, 823, 1323
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 4 cut(s) 47, 127, 991, 1092
BssT1I CCWWGG 1 cut(s) 649
Bst2UI CCWGG 2 cut(s) 135, 924
Bst4CI ACNGT 4 cut(s) 281, 760, 779, 854
BstC8I GCNNGC 2 cut(s) 448, 933
BstDEI CTNAG 8 cut(s) 12, 161, 258, 477, 609, 615, 1176, 1290
BstENI CCTNNNNNAGG 2 cut(s) 76, 666
BstF5I GGATG 5 cut(s) 87, 205, 460, 466, 856
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 4 cut(s) 50, 130, 994, 1095
BstMAI GTCTC 5 cut(s) 240, 278, 618, 1166, 1280
BstMBI GATC 4 cut(s) 47, 127, 991, 1092
BstMWI GCNNNNNNNGC 3 cut(s) 385, 749, 1157
BstNI CCWGG 2 cut(s) 135, 924
BstNSI RCATGY 3 cut(s) 315, 418, 939
BstPAI GACNNNNGTC 1 cut(s) 1191
BstSCI CCNGG 2 cut(s) 133, 922
BstSFI CTRYAG 2 cut(s) 1152, 1311
BstV1I GCAGC 4 cut(s) 28, 990, 1135, 1138
BstV2I GAAGAC 2 cut(s) 102, 1032
BstX2I RGATCY 1 cut(s) 1092
BstYI RGATCY 1 cut(s) 1092
BsuRI GGCC 7 cut(s) 306, 492, 620, 654, 677, 953, 1034
BtgZI GCGATG 1 cut(s) 1083
BtrI CACGTC 1 cut(s) 596
BtsCI GGATG 5 cut(s) 87, 205, 460, 466, 856
BtsIMutI CAGTG 3 cut(s) 179, 550, 816
BveI ACCTGC 1 cut(s) 10
Cac8I GCNNGC 2 cut(s) 448, 933
CaiI CAGNNNCTG 1 cut(s) 1289
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 3 cut(s) 305, 350, 675
CviAII CATG 8 cut(s) 312, 402, 415, 634, 685, 936, 1036, 1359
DdeI CTNAG 8 cut(s) 12, 161, 258, 477, 609, 615, 1176, 1290
DpnI GATC 4 cut(s) 49, 129, 993, 1094
DpnII GATC 4 cut(s) 47, 127, 991, 1092
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 490, 1032
Eco130I CCWWGG 1 cut(s) 649
Eco147I AGGCCT 3 cut(s) 620, 654, 953
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 58
EcoNI CCTNNNNNAGG 2 cut(s) 76, 666
EcoRII CCWGG 2 cut(s) 133, 922
EcoT14I CCWWGG 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 8 cut(s) 315, 405, 418, 637, 688, 939, 1039, 1362
FatI CATG 8 cut(s) 311, 401, 414, 633, 684, 935, 1035, 1358
FauI CCCGC 1 cut(s) 1153
FauNDI CATATG 2 cut(s) 502, 580
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 5 cut(s) 17, 386, 1004, 1149, 1152
FokI GGATG 5 cut(s) 94, 192, 447, 453, 843
Fsp4HI GCNGC 5 cut(s) 17, 386, 1004, 1149, 1152
FspBI CTAG 2 cut(s) 114, 738
GlaI GCGC 1 cut(s) 742
GluI GCNGC 5 cut(s) 17, 386, 1004, 1149, 1152
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 1 cut(s) 715
HaeIII GGCC 7 cut(s) 306, 492, 620, 654, 677, 953, 1034
HhaI GCGC 1 cut(s) 743
Hin1II CATG 8 cut(s) 315, 405, 418, 637, 688, 939, 1039, 1362
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HindIII AAGCTT 1 cut(s) 1165
HinfI GANTC 7 cut(s) 43, 435, 728, 1226, 1250, 1307, 1340
HphI GGTGA 5 cut(s) 149, 314, 404, 848, 873
Hpy166II GTNNAC 1 cut(s) 1142
Hpy188III TCNNGA 4 cut(s) 53, 76, 125, 895
Hpy8I GTNNAC 1 cut(s) 1142
HpyAV CCTTC 2 cut(s) 82, 492
HpyCH4III ACNGT 4 cut(s) 281, 760, 779, 854
HpyCH4IV ACGT 1 cut(s) 595
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 749, 1157
HpyF3I CTNAG 8 cut(s) 12, 161, 258, 477, 609, 615, 1176, 1290
HpySE526I ACGT 1 cut(s) 595
Hsp92II CATG 8 cut(s) 315, 405, 418, 637, 688, 939, 1039, 1362
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 4 cut(s) 47, 127, 991, 1092
LmnI GCTCC 5 cut(s) 106, 290, 584, 974, 1072
Lsp1109I GCAGC 4 cut(s) 28, 990, 1135, 1138
LweI GCATC 3 cut(s) 701, 752, 1270
MaeI CTAG 2 cut(s) 114, 738
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 4 cut(s) 754, 760, 873, 941
MalI GATC 4 cut(s) 49, 129, 993, 1094
MboI GATC 4 cut(s) 47, 127, 991, 1092
MboII GAAGA 5 cut(s) 77, 107, 212, 902, 1032
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 1092
MlsI TGGCCA 2 cut(s) 492, 1034
MluCI AATT 5 cut(s) 60, 251, 452, 986, 1084
MluNI TGGCCA 2 cut(s) 492, 1034
MlyI GAGTC 2 cut(s) 722, 1220
MmeI TCCRAC 2 cut(s) 247, 1220
Mox20I TGGCCA 2 cut(s) 492, 1034
MscI TGGCCA 2 cut(s) 492, 1034
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 683
Msp20I TGGCCA 2 cut(s) 492, 1034
MspA1I CMGCKG 1 cut(s) 1148
MspR9I CCNGG 2 cut(s) 135, 924
MunI CAATTG 1 cut(s) 251
MvaI CCWGG 2 cut(s) 135, 924
MwoI GCNNNNNNNGC 3 cut(s) 385, 749, 1157
NdeI CATATG 2 cut(s) 502, 580
NdeII GATC 4 cut(s) 47, 127, 991, 1092
NlaIII CATG 8 cut(s) 315, 405, 418, 637, 688, 939, 1039, 1362
NlaIV GGNNCC 2 cut(s) 292, 352
NmuCI GTSAC 2 cut(s) 754, 760
NspI RCATGY 3 cut(s) 315, 418, 939
PceI AGGCCT 3 cut(s) 620, 654, 953
PciI ACATGT 1 cut(s) 311
PfeI GAWTC 5 cut(s) 43, 435, 1250, 1307, 1340
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 5 cut(s) 18, 387, 1005, 1150, 1153
PleI GAGTC 2 cut(s) 722, 1220
PpsI GAGTC 2 cut(s) 722, 1220
PscI ACATGT 1 cut(s) 311
PshAI GACNNNNGTC 1 cut(s) 1191
PsiI TTATAA 1 cut(s) 1017
Psp6I CCWGG 2 cut(s) 133, 922
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 2 cut(s) 133, 922
PspN4I GGNNCC 2 cut(s) 292, 352
PspPI GGNCC 3 cut(s) 305, 350, 675
PstI CTGCAG 2 cut(s) 1156, 1315
PstNI CAGNNNCTG 1 cut(s) 1289
PsuI RGATCY 1 cut(s) 1092
RseI CAYNNNNRTG 1 cut(s) 683
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 5 cut(s) 17, 386, 1004, 1149, 1152
Sau3AI GATC 4 cut(s) 47, 127, 991, 1092
Sau96I GGNCC 3 cut(s) 305, 350, 675
SchI GAGTC 2 cut(s) 722, 1220
ScrFI CCNGG 2 cut(s) 135, 924
SfaNI GCATC 3 cut(s) 701, 752, 1270
SfcI CTRYAG 2 cut(s) 1152, 1311
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 683
SmlI CTYRAG 2 cut(s) 600, 666
SmoI CTYRAG 2 cut(s) 600, 666
Sse9I AATT 5 cut(s) 60, 251, 452, 986, 1084
SseBI AGGCCT 3 cut(s) 620, 654, 953
SsiI CCGC 2 cut(s) 385, 1146
SspMI CTAG 2 cut(s) 114, 738
StuI AGGCCT 3 cut(s) 620, 654, 953
StyD4I CCNGG 2 cut(s) 133, 922
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 4 cut(s) 281, 760, 779, 854
TaiI ACGT 1 cut(s) 598
TaqI TCGA 5 cut(s) 41, 46, 196, 433, 726
TasI AATT 5 cut(s) 60, 251, 452, 986, 1084
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 5 cut(s) 43, 435, 1250, 1307, 1340
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 3 cut(s) 179, 550, 823
TseFI GTSAC 2 cut(s) 754, 760
TseI GCWGC 4 cut(s) 16, 1003, 1148, 1151
Tsp45I GTSAC 2 cut(s) 754, 760
TspDTI ATGAA 5 cut(s) 98, 108, 728, 827, 1332
TspGWI ACGGA 2 cut(s) 510, 972
TspRI CASTG 3 cut(s) 179, 550, 823
Van91I CCANNNNNTGG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 350
XagI CCTNNNNNAGG 2 cut(s) 76, 666
XapI RAATTY 2 cut(s) 452, 986
XceI RCATGY 3 cut(s) 315, 418, 939
XspI CTAG 2 cut(s) 114, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.