AT1G08260

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001971
Physical Location & Seq
Forward (+)
2588854 .. 2606892
18039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08260.1

Sequence Viewer

Length: 6486 bp
ATGAGCGGAGATAATCGAAGACGGGATCGAAAGGATACGCGGTGGTCGAAGAAGCCTAAGGTAGTGAACACTGCGGAAGATGAACTCGAATCTAAACTCGGATTTGGGCTTTTCTCTGAAGGTGAAACCCGACTTGGGTGGTTACTCACATTCTCATCGTCATCTTGGGAAGATCGCGATACTGGGAAAGTCTATAGTTGTGTGGACCTTTACTTTGTTACTCAGGACGGCTTTTCTTTCAAGACAAAGTACAAGTTCCGTCCTTACTTTTATGCAGCCACGAAAGACAAAATGGAATTGGAACTCGAAGCCTACTTGAGACGCCGATATGAAAGACAAGTTGCTGATATTGAGATTGTTGAAAAAGAAGATCTTGATCTTAAAAATCATCTATCTGGCCTGCAAAAAAAATACCTAAAAATATCATTTGATACTGTGCAACAATTGATGGAAGTCAAGCGGGATTTGTTACATATTGTCGAACGAAACCAGGCAAAATTTGATGCACTTGAAGCATACGAATCCATATTGGCTGGAAAACGAGAACAACGTCCTCAAGATTGCTTAGACTCTATCGTGGACCTCCGCGAGTACGATGTACCTTATCATGTTCGATTTGCAATTGATAATGATGTGCGGAGTGGGCAATGGTACAATGTCAGTATTTCCAGCACCGATGTCATATTGGAGAAGAGGACAGATCTTCTTCAACGCGCAGAAGTCCGTGTTTGTGCCTTCGATATAGAGACAACAAAGCTTCCGTTGAAGTTTCCTGATGCTGAATATGATCAAATTATGATGATATCCTACATGGTCGATGGGCAAGGTTTTCTAATTATTAACAGAGAGTGTGTTGGCGAAGATGTAGAAGATCTGGAATATACACCAAAACCAGAATTTGAAGGGTATTTCAAAGTTACTAATGTTAAAAATGAGGTTGAACTCCTCCAAAGATGGTTTTACCATATGCAAGAGTTGAAACCCGGTATTTATGTTACATACAACGGTGACTTTTTTGACTGGCCATTTATAGAACGGAGAGCATCTCATCATGGGATAAAAATGAATGAGGAATTGGGATTCCGTTGTGATCAGAATCAAGGTGAATGTCGAGCTAAATTCGCTTGTCATTTGGATTGTTTTGCTTGGGTTAAACGTGATAGCTATCTTCCTCAGGGAAGCCATGGTCTGAAGGCTGTTACAAAGGCAAAACTAGGTTATGATCCACTGGAAGTGAACCCAGAGGACATGGTTCGGTTTGCGATGGAAAAGCCCCAGACAATGGCCTCCTACTCTGTCTCTGATGCTGTTGCAACTTATTACCTATACATGACATATGTCAATCCATTCATTTTTTCTCTCGCAACTATCATCCCAATGGTCCCTGATGAGGTTTTACGTAAAGGGAGTGGCACCCTTTGTGAAATGCTTCTGATGGTTGAGGCTTACAAGGCAAATGTTGTATGTCCCAACAAAAATCAAGCTGACCCTGAGAAATTCTACCAAAATCAACTTCTTGAAAGTGAGACATATATTGGTGGTCATGTAGAGTGCCTTGAAAGTGGTGTTTTCAGATCTGACATTCCAACCAGTTTTAAACTCGATTCATCGGCATACCAGCAACTGATCGATAACCTTGGTCGTGATCTGGAATATGCTATCACTGTAGAAGGAAAAATGAGAATGGATTCAATTTCCAATTATGATGAAGTGAAGGACGAAATCAAGGAGAAGCTTGAGAAGTTACGAGATGACCCTATACGGGAAGAGGGGCCTCTTATTTATCACCTTGATGTCGCAGCCATGTATCCAAATATCATCTTAACAAACAGGCTTCAGCCACCATCAATAGTTACGGATGAGATTTGCACAGCATGTGATTTCAACCGCCCGGGAAAGACCTGTCTTAGAAAACTTGAATGGGTTTGGCGTGGTGTGACATTCATGGGAAAGAAAAGTGATTATTACCATTTGAAGAAGCAGATTGAGTCTGAATTTGTTGATGCTGGTGCAAATATAATGTCTTCAAAATCGTTCCTTGATCTGCCAAAGGTGGATCAACAATCTAAGCTAAAAGAACGACTAAAAAAATATTGTCAAAAGGCTTATAAACGAGTACTTGACAAGCCAATTACTGAAGTTCGTGAAGCTGGGATATGCATGAGAGAAAACCCTTTCTATGTGGATACTGTTCGGAGCTTTCGAGATAGGAGGTATGAATACAAAACACTTAACAAGGTCTGGAAGGGAAAATTGTCAGAGGCCAAGGCAAGTGGTAATTCAATCAAGATCCAGGAAGCACAGGACATGGTAGTGGTTTACGATTCCTTGCAGCTAGCTCATAAGTGTATACTTAATTCCTTCTATGGATATGTCATGCGAAAGGGTGCAAGATGGTATTCCATGGAAATGGCTGGTGTAGTTACCTATACTGGAGCCAAAATCATACAGAATGCCCGTTTGCTTATTGAGCGAATTGGGAAACCACTTGAGTTGGATACAGATGGTATATGGTGTTGTCTCCCTGGATCTTTTCCTGAGAACTTTACTTTTAAAACAATAGACATGAAGAAACTCACCATCTCGTATCCTTGTGTTATGCTTAATGTTGATGTGGCAAAGAATAATACAAATGATCAATATCAGACTCTTGTAGATCCTGTACGCAAGACATATAAATCACATAGTGAATGCTCAATTGAATTTGAAGTAGATGGACCGTACAAGGCAATGATTATTCCTGCATCGAAAGAAGAAGGAATCTTAATCAAGAAGCGTTATGCTGTTTTCAATCATGATGGAACCTTAGCAGAACTCAAGGGGTTTGAGATCAAGCGTAGAGGGGAGCTGAAACTCATTAAAGTTTTCCAGGCCGAGCTTTTTGACAAATTTCTCCATGGATCAACTCTTGAAGAGTGTTATTCTGCTGTTGCAGCGGTTGCTGATCGATGGCTTGACCTACTCGATAATCAAGGAAAAGATATTGCCGATAGTGAACTGCTAGATTATATATCAGAATCAAGCACAATGAGCAAATCGTTAGCAGATTATGGTGAGCAAAAGTCATGTGCAGTGACCACAGCAAAACGCCTGGCTGAATTTCTTGGTGTTACAATGGTCAAAGATAAAGGACTACGTTGCCAGTACATTGTTGCTTGTGAACCAAAGGGTACACCGGTAAGCGAACGTGCTGTTCCTGTTGCCATATTTACAACAAATCCGGAAGTTATGAAGTTTCATTTGCGAAAATGGTGCAAGACATCATCAGATGTCGGAATTCGTCTGATTATTGACTGGTCGTATTACAAGCAGCGCCTTAGTTCAGCTATCCAAAAAGTTATTACCATTCCTGCTGCAATGCAGAAGGTGGCAAATCCCGTGCCTAGGGTGCTTCATCCTGACTGGCTCCATAAAAAGGTCCGTGAGAAAGACGACAAATTTCGCCAGAGAAAACTAGTTGATATGTTCAGCTCAGCAAATAAAGATGTTGTGTTGGACACAGATCTTCCGGTGACTAAAGATAATGTGGAAGATATTGAGGATTTTTGCAAAGAAAATAGGCCTTCCGTCAAAGGGCCAAAGCCAATTGCGCGTTCCTATGAAGTCAATAAAAAACAGTCTGAATGTGAGCAGCAAGAGAGCTGGGATACAGAGTTCCATGATATCTCATTTCAGAACATTGACAAGAGCGTAAACTACCAAGGATGGCTTGAACTGAAAAAGAGAAAATGGAAAGTAACTCTAGAGAAGAAGAAAAAACGAAGGTTGGGTGATCTAAGATCTTCAAACCAGGTTGATACTCACGAGATAAATCAAAAAGTCGGTCAGGGAAGAGGAGGTGTGGGTTCATACTTTAGGAGGCCTGAAGAAGCTTTGACTAGTTCACATTGGCAGATAATACAGCTGGTCCCAAGTCCACAGAGCGGACAATTTTTTGCTTGGGTGGTTGTGGAAGGGTTGATGCTTAAGATCCCATTGTCGATCCCAAGGGTGTTTTACATTAATTCCAAAGTTCCTATAGATGAATATTTCCAAGGAAAGTGTGTCAACAAGATTCTTCCTCATGGAAGGCCTTGTTATAGTTTGACTGAGGTCAAGATTCAAGAAGATCAATTTAAAAAAGAAAGTAAAAAGCGTGCAGCTCTTCTTGCAGACCCTGGAGTTGAGGGCATATATGAAACTAAGGTGCCTCTGGAGTTTAGTGCTATATGTCAGATTGGATGTGTATGCAAAATTGATAACAAAGCCAAGCACCGTAACACCCAAGATGGATGGGAAGTTGGTGAACTTCACATGAAAACTACAACTGAGTGTCACTACTTGAAGCGTTCAATTCCACTTGTTTACTTGTATAACAGCACCTCAACAGGGCGTGCTATATATGTTTTATATTGTCATGTATCGAAGCTTATGTCTGCCGTTGTAGTTGATCCTTTTAACGGCAATGAATTATTACCATCTGCCTTAGAGAGACAGTTTAGAGATAGTTGCCTAGAATTATCACTTGATTCATTGTCTTGGGATGGTATCCGTTTCCAGGTGCACTACGTTGACCATCCCGAAGCTGCCAAGAAGATAATACAAAGAGCAATAAGTGAATACAGAGAAGAAAATTGTGGACCAACTGTTGCTGTCATTGAATGCCCTGACTTTACCTTCATGAAGGAAGGTATTAAGGCCCTAGATGATTTCCCATGTGTGAGGATTCCCTTCAACGACGATGACAATAGTTATCAGCCTGTCTCCTGGCAACGTCCAGCTGCAAAAATAGCAATGTTCCGTTGTGCTGCAGCATTTCAGTGGTTGGATCGGAGGATTACCCAATCAAGATACGCCCATGTGCCTCTGGGGAATTTTGGACTTGATTGGCTAACATTCACAATAGATATCTTCCTATCCAGGGCACTCCGTGACCAGCAACAGGTCCTTTGGGTGTCAGATAATGGTGTTCCAGACCTTGGAGGTATAAACAATGAAGAGGCATTCTTTGCCGATGAGGTGCAACAGACAAGTTTAGTATTCCCTGGAGCGTATAGAAAAGTGTCTGTGGAGTTAAAGATCCATAACTTGGCTGTTAATGCCCTTCTGAAAAGCAACCTGGTGAATGAAATGGAAGGAGGCGGTTTTATGGGATTTGAACAGGATGTGAATCCTAGAGGAATTAATTCAAACGATAACACCAGTTTTGATGAGACCACTGGATGTGCTCAAGCATTTCGTGTCCTGAAACAGTTGATTCATAGTTGCTTGACTGATGTGCGTAAATCTAAAAATATATATGCTGATTCCATTTTGCAACGTTTGTCTTGGTGGCTCTGCAGCCCTTCCTCGAAGCTCCATGATCCAGCTCTTCATCTCATGCTTCACAAGGTCATGCAAAAGGTGTTTGCACTGCTATTGACTGATTTGCGGAGATTAGGTGCTATAATAATATATGCAGACTTCTCAAAGGTCATTATCGACACAGTGAAATTTGATCTATCTGCAGCTAAGGCTTATTGTGAAAGCTTGCTATCAACGGTGCGGAATAGTGATATCTTTGAGTGGATATTGCTCGAGCCGGTTCACTACTGGCACTCATTACTTTTCATGGACCAGTATAACTACGCTGGTATCCGAGCCGATGATGAAATTTCACTTGACGAAGTGACTATTGAACCAAAGTGGAGTGTTGCACGACATTTACCTGAGTATATTGAGAGGGATTTCATTATAATCATTGCCAAGTTTATATTTGACCCCTGGAAATTTGCGATAGAAAATAAAAAGGGCAGTTCAGAGAGTTTAGAGGCACAGATGATAGAATATCTAAGAGAACAGATTGGATCAACCTTCATAAATATGCTTGTCAAAAAGGTTGATGATATCATGTCACACATGAAGGAGATAAATGTTTCAGATGCATCTCGGGTTTCTGGTCAAGCTCCCAAAGGAGATTACTCGTTGGAATTTATACAGGTTATCTCTGCTGTTCTGGCTCTTGACCAAAACGTCCAGCAAGATGTTCTGGTAATGAGAAAGAGTCTGTTGAAGTACATTAAAGTAAAAGAGTGTGCTGCTGAAGCCGAATTTCTTGATCCTGGGCCATCCTTCATCTTACCTAATGTAGCTTGCAGCAACTGTGATGCCTACAGAGACCTAGATATATGCAGAGACCCAGCACTGTTAACAGAGAAAGAATGGTCATGTGCGGATACACAATGTGGGAAGATATATGACAGAGAGCAGATGGAGAGTAGCCTTCTAGAGATGGTGAGACAGAGAGAAAGAATGTACCATATGCAGGATGTAGTGTGCATTAGGTGCAATCAGGTGAAAGCGGCTCATTTGACAGAGCAGTGTGAGTGTTCTGGTTCCTTTAGATGCAAGGAGAGTGGGTCAGAGTTCAGCAAGAGGATGGAGATATTCATGGATATAGCAAAGCGCCAGAAGTTTAGACTGTTAGAAGAATACATCTCTTGGATCATATATGGCCCTAGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009987 GO:0010015 GO:0010086 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022616 GO:0022622 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0048046 GO:0048364 GO:0048519 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048598 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051716 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0097159 GO:0099402 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1905392 GO:1990234 GO:2000026 GO:2000028 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2161

Amino Acids

248.89

Weight (kDa)

6.19

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 194 - 400 6.5e-62 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 742 - 1071 5.1e-17 DNA polymerase family B
POL2_thumb PF22634 946 - 1121 4.3e-76 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1509 - 1872 2.2e-111 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2021 - 2088 8.8e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2094 - 2150 3.5e-14 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2109, 5714
AasI GACNNNNNNGTC 1 cut(s) 5990
AccB1I GGYRCC 2 cut(s) 1412, 4186
AccB7I CCANNNNNTGG 3 cut(s) 1282, 4958, 5068
AccBSI CCGCTC 2 cut(s) 6, 3924
AccI GTMKAC 1 cut(s) 2350
AccII CGCG 5 cut(s) 40, 177, 588, 714, 3592
AccIII TCCGGA 1 cut(s) 3220
AclI AACGTT 1 cut(s) 5299
AcoI YGGCCR 1 cut(s) 1022
AcuI CTGAAG 6 cut(s) 138, 1211, 1820, 2157, 3885, 6081
AcyI GRCGYC 1 cut(s) 322
AdeI CACNNNGTG 3 cut(s) 2687, 4910, 6203
AflII CTTAAG 1 cut(s) 3965
AgeI ACCGGT 1 cut(s) 3175
AhlI ACTAGT 2 cut(s) 3454, 3878
AjuI GAANNNNNNNTTGG 8 cut(s) 117, 149, 1058, 1090, 1497, 1529, 3577, 3609
AloI GAACNNNNNNTCC 2 cut(s) 3638, 3670
Alw21I GWGCWC 2 cut(s) 4545, 5209
Alw44I GTGCAC 1 cut(s) 4541
AlwNI CAGNNNCTG 3 cut(s) 1624, 4157, 6120
Ama87I CYCGRG 3 cut(s) 1891, 5555, 5907
Aor13HI TCCGGA 1 cut(s) 3220
ApaLI GTGCAC 1 cut(s) 4541
ArsI GACNNNNNNTTYG 8 cut(s) 197, 229, 2006, 2038, 3808, 3840, 4397, 4429
AseI ATTAAT 2 cut(s) 4002, 5163
AsiGI ACCGGT 1 cut(s) 3175
Asp700I GAANNNNTTC 3 cut(s) 1428, 1687, 5164
AspA2I CCTAGG 1 cut(s) 3383
AspLEI GCGC 4 cut(s) 716, 3315, 3592, 6426
AsuC2I CCSGG 3 cut(s) 984, 1892, 1893
AsuNHI GCTAGC 1 cut(s) 2335
AvaI CYCGRG 3 cut(s) 1891, 5555, 5907
AvaII GGWCC 9 cut(s) 205, 580, 1381, 2717, 3418, 3907, 4619, 4924, 5593
AvrII CCTAGG 1 cut(s) 3383
AxyI CCTNAGG 2 cut(s) 57, 1173
BaeGI GKGCMC 2 cut(s) 4545, 4906
BaeI ACNNNNGTAYC 4 cut(s) 3162, 3195, 5596, 5629
BalI TGGCCA 1 cut(s) 1024
BanI GGYRCC 2 cut(s) 1412, 4186
BauI CACGAG 1 cut(s) 3803
BbsI GAAGAC 2 cut(s) 25, 2016
Bbv12I GWGCWC 2 cut(s) 4545, 5209
BceAI ACGGC 3 cut(s) 244, 4403, 4456
BcgI CGANNNNNNTGC 2 cut(s) 3234, 3268
BciVI GTATCC 9 cut(s) 28, 1818, 2179, 2491, 2598, 3640, 4538, 5624, 6187
BclI TGATCA 3 cut(s) 787, 1090, 2635
BcnI CCSGG 3 cut(s) 984, 1892, 1893
BcuI ACTAGT 2 cut(s) 3454, 3878
BfmI CTRYAG 7 cut(s) 193, 1665, 4017, 4788, 5317, 5484, 6130
BfoI RGCGCY 2 cut(s) 3316, 6427
BfrI CTTAAG 1 cut(s) 3965
BfuI GTATCC 9 cut(s) 28, 1818, 2179, 2491, 2598, 3640, 4538, 5624, 6187
BglII AGATCT 6 cut(s) 370, 700, 871, 1574, 3502, 3779
BlnI CCTAGG 1 cut(s) 3383
BlpI GCTNAGC 1 cut(s) 3472
BmcAI AGTACT 1 cut(s) 2118
Bme18I GGWCC 9 cut(s) 205, 580, 1381, 2717, 3418, 3907, 4619, 4924, 5593
BmeT110I CYCGRG 3 cut(s) 1891, 5555, 5907
BmiI GGNNCC 9 cut(s) 1383, 1414, 1773, 2437, 2803, 3407, 3909, 4188, 6355
BmrI ACTGGG 1 cut(s) 192
BmtI GCTAGC 1 cut(s) 2339
BmuI ACTGGG 1 cut(s) 192
BoxI GACNNNNGTC 2 cut(s) 1337, 4091
BpiI GAAGAC 2 cut(s) 25, 2016
BplI GAGNNNNNCTC 2 cut(s) 1030, 1062
BpmI CTGGAG 4 cut(s) 2454, 4179, 4214, 5046
Bpu10I CCTNAGC 2 cut(s) 2806, 5490
Bpu1102I GCTNAGC 1 cut(s) 3472
BpuEI CTTGAG 6 cut(s) 337, 540, 1757, 2510, 2801, 5193
BpuMI CCSGG 3 cut(s) 984, 1892, 1893
Bsa29I ATCGAT 2 cut(s) 1629, 2947
BsaAI YACGTR 1 cut(s) 1400
BsaBI GATNNNNATC 5 cut(s) 375, 1095, 1164, 2640, 5148
BsaHI GRCGYC 1 cut(s) 322
BsaI GGTCTC 3 cut(s) 5186, 6129, 6147
BsaWI WCCGGW 3 cut(s) 3175, 3220, 3508
BsaXI ACNNNNNCTCC 4 cut(s) 5404, 5434, 6362, 6392
Bse118I RCCGGY 2 cut(s) 3175, 5560
Bse21I CCTNAGG 2 cut(s) 57, 1173
Bse3DI GCAATG 6 cut(s) 653, 2736, 3363, 4450, 4779, 5718
Bse8I GATNNNNATC 5 cut(s) 375, 1095, 1164, 2640, 5148
BseAI TCCGGA 1 cut(s) 3220
BseCI ATCGAT 2 cut(s) 1629, 2947
BseJI GATNNNNATC 5 cut(s) 375, 1095, 1164, 2640, 5148
BseMI GCAATG 6 cut(s) 653, 2736, 3363, 4450, 4779, 5718
BseMII CTCAG 8 cut(s) 236, 1187, 1482, 2529, 3486, 4080, 4299, 5679
BseRI GAGGAG 2 cut(s) 935, 3849
BseSI GKGCMC 2 cut(s) 4545, 4906
BseYI CCCAGC 3 cut(s) 2150, 3642, 6157
BsgI GTGCAG 2 cut(s) 3090, 4158
Bsh1236I CGCG 5 cut(s) 40, 177, 588, 714, 3592
BshNI GGYRCC 2 cut(s) 1412, 4186
BshTI ACCGGT 1 cut(s) 3175
BshVI ATCGAT 2 cut(s) 1629, 2947
BsiHKAI GWGCWC 2 cut(s) 4545, 5209
BsiHKCI CYCGRG 3 cut(s) 1891, 5555, 5907
BsiSI CCGG 6 cut(s) 984, 1892, 3176, 3221, 3509, 5561
BslFI GGGAC 3 cut(s) 1367, 1452, 3893
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 3 cut(s) 1367, 1452, 3893
BsmI GAATGC 4 cut(s) 2458, 2696, 4646, 4982
Bso31I GGTCTC 3 cut(s) 5186, 6129, 6147
BsoBI CYCGRG 3 cut(s) 1891, 5555, 5907
Bsp1286I GDGCHC 3 cut(s) 4545, 4906, 5209
Bsp13I TCCGGA 1 cut(s) 3220
Bsp1720I GCTNAGC 1 cut(s) 3472
Bsp19I CCATGG 3 cut(s) 1183, 2403, 2896
Bsp68I TCGCGA 1 cut(s) 177
BspCNI CTCAG 8 cut(s) 235, 1186, 1483, 2530, 3485, 4081, 4300, 5680
BspDI ATCGAT 2 cut(s) 1629, 2947
BspEI TCCGGA 1 cut(s) 3220
BspFNI CGCG 5 cut(s) 40, 177, 588, 714, 3592
BspHI TCATGA 2 cut(s) 2794, 4659
BspLI GGNNCC 9 cut(s) 1383, 1414, 1773, 2437, 2803, 3407, 3909, 4188, 6355
BspMAI CTGCAG 3 cut(s) 4792, 5321, 5488
BspOI GCTAGC 1 cut(s) 2339
BspQI GCTCTTC 2 cut(s) 4149, 5355
BspT107I GGYRCC 2 cut(s) 1412, 4186
BspTI CTTAAG 1 cut(s) 3965
BspTNI GGTCTC 3 cut(s) 5186, 6129, 6147
BsrBI CCGCTC 2 cut(s) 6, 3924
BsrDI GCAATG 6 cut(s) 653, 2736, 3363, 4450, 4779, 5718
BsrFI RCCGGY 2 cut(s) 3175, 5560
BssAI RCCGGY 2 cut(s) 3175, 5560
BssNAI GTATAC 1 cut(s) 2351
BssNI GRCGYC 1 cut(s) 322
BssSI CACGAG 1 cut(s) 3803
Bst1107I GTATAC 1 cut(s) 2351
Bst2BI CACGAG 1 cut(s) 3803
Bst6I CTCTTC 7 cut(s) 686, 1761, 2907, 3826, 4149, 4971, 5355
BstACI GRCGYC 1 cut(s) 322
BstAFI CTTAAG 1 cut(s) 3965
BstAPI GCANNNNNTGC 3 cut(s) 2939, 4988, 6303
BstBAI YACGTR 1 cut(s) 1400
BstC8I GCNNGC 6 cut(s) 401, 2337, 4137, 4374, 5510, 6112
BstDSI CCRYGG 3 cut(s) 1183, 2403, 2896
BstFNI CGCG 5 cut(s) 40, 177, 588, 714, 3592
BstH2I RGCGCY 2 cut(s) 3316, 6427
BstHHI GCGC 4 cut(s) 716, 3315, 3592, 6426
BstNSI RCATGY 1 cut(s) 1878
BstPAI GACNNNNGTC 2 cut(s) 1337, 4091
BstSFI CTRYAG 7 cut(s) 193, 1665, 4017, 4788, 5317, 5484, 6130
BstSLI GKGCMC 2 cut(s) 4545, 4906
BstSNI TACGTA 1 cut(s) 1400
BstUI CGCG 5 cut(s) 40, 177, 588, 714, 3592
BstV2I GAAGAC 2 cut(s) 25, 2016
BstXI CCANNNNNNTGG 1 cut(s) 2410
BstZ17I GTATAC 1 cut(s) 2351
Bsu15I ATCGAT 2 cut(s) 1629, 2947
Bsu36I CCTNAGG 2 cut(s) 57, 1173
BsuI GTATCC 9 cut(s) 28, 1818, 2179, 2491, 2598, 3640, 4538, 5624, 6187
BsuTUI ATCGAT 2 cut(s) 1629, 2947
BtgI CCRYGG 3 cut(s) 1183, 2403, 2896
BtgZI GCGATG 1 cut(s) 1277
BtsI GCAGTG 4 cut(s) 69, 3078, 5390, 6344
BtuMI TCGCGA 1 cut(s) 177
Cac8I GCNNGC 6 cut(s) 401, 2337, 4137, 4374, 5510, 6112
CaiI CAGNNNCTG 3 cut(s) 1624, 4157, 6120
CciI TCATGA 2 cut(s) 2794, 4659
CfoI GCGC 4 cut(s) 716, 3315, 3592, 6426
Cfr10I RCCGGY 2 cut(s) 3175, 5560
Cfr9I CCCGGG 1 cut(s) 1891
ClaI ATCGAT 2 cut(s) 1629, 2947
CseI GACGC 1 cut(s) 330
CsiI ACCWGGT 2 cut(s) 3789, 5097
CspAI ACCGGT 1 cut(s) 3175
CspCI CAANNNNNGTGG 6 cut(s) 2254, 2289, 2475, 2510, 6355, 6390
DraI TTTAAA 3 cut(s) 1597, 2554, 4117
DraIII CACNNNGTG 3 cut(s) 2687, 4910, 6203
DrdI GACNNNNNNGTC 1 cut(s) 5990
DseDI GACNNNNNNGTC 1 cut(s) 5990
EaeI YGGCCR 1 cut(s) 1022
Eam1104I CTCTTC 7 cut(s) 686, 1761, 2907, 3826, 4149, 4971, 5355
EarI CTCTTC 7 cut(s) 686, 1761, 2907, 3826, 4149, 4971, 5355
Eco105I TACGTA 1 cut(s) 1400
Eco147I AGGCCT 3 cut(s) 3562, 3862, 4072
Eco31I GGTCTC 3 cut(s) 5186, 6129, 6147
Eco32I GATATC 5 cut(s) 804, 3664, 4888, 5536, 5866
Eco47I GGWCC 9 cut(s) 205, 580, 1381, 2717, 3418, 3907, 4619, 4924, 5593
Eco57I CTGAAG 6 cut(s) 138, 1211, 1820, 2157, 3885, 6081
Eco81I CCTNAGG 2 cut(s) 57, 1173
Eco88I CYCGRG 3 cut(s) 1891, 5555, 5907
EcoO109I RGGNCCY 3 cut(s) 1772, 4678, 4924
EcoRI GAATTC 1 cut(s) 3276
EcoRV GATATC 5 cut(s) 804, 3664, 4888, 5536, 5866
EcoT22I ATGCAT 2 cut(s) 2162, 5905
Esp3I CGTCTC 1 cut(s) 313
FalI AAGNNNNNCTT 4 cut(s) 357, 389, 3693, 3725
FaqI GGGAC 3 cut(s) 1367, 1452, 3893
FauI CCCGC 1 cut(s) 453
FauNDI CATATG 3 cut(s) 966, 1336, 6279
FbaI TGATCA 3 cut(s) 787, 1090, 2635
FblI GTMKAC 1 cut(s) 2350
GlaI GCGC 4 cut(s) 715, 3314, 3591, 6425
GsaI CCCAGC 3 cut(s) 2154, 3646, 6161
GsuI CTGGAG 4 cut(s) 2454, 4179, 4214, 5046
HaeII RGCGCY 2 cut(s) 3316, 6427
HapII CCGG 6 cut(s) 984, 1892, 3176, 3221, 3509, 5561
HgaI GACGC 1 cut(s) 330
HhaI GCGC 4 cut(s) 716, 3315, 3592, 6426
Hin1I GRCGYC 1 cut(s) 322
Hin6I GCGC 4 cut(s) 714, 3313, 3590, 6424
HinP1I GCGC 4 cut(s) 714, 3313, 3590, 6424
HincII GTYRAC 3 cut(s) 4048, 4552, 6168
HindII GTYRAC 3 cut(s) 4048, 4552, 6168
HindIII AAGCTT 5 cut(s) 755, 1733, 3870, 4406, 5506
HpaI GTTAAC 1 cut(s) 6168
HpaII CCGG 6 cut(s) 984, 1892, 3176, 3221, 3509, 5561
Hpy99I CGWCG 1 cut(s) 4721
HpyCH4IV ACGT 9 cut(s) 550, 1156, 1399, 3136, 3187, 4548, 4753, 5299, 5991
HpySE526I ACGT 9 cut(s) 550, 1156, 1399, 3136, 3187, 4548, 4753, 5299, 5991
Hsp92I GRCGYC 1 cut(s) 322
HspAI GCGC 4 cut(s) 714, 3313, 3590, 6424
Kpn2I TCCGGA 1 cut(s) 3220
Ksp22I TGATCA 3 cut(s) 787, 1090, 2635
KspAI GTTAAC 1 cut(s) 6168
LguI GCTCTTC 2 cut(s) 4149, 5355
LmnI GCTCC 7 cut(s) 2196, 2435, 2845, 3411, 5027, 5340, 5929
MabI ACCWGGT 2 cut(s) 3789, 5097
MaeII ACGT 9 cut(s) 550, 1156, 1399, 3136, 3187, 4548, 4753, 5299, 5991
MbiI CCGCTC 2 cut(s) 6, 3924
MfeI CAATTG 4 cut(s) 443, 621, 2697, 3585
MhlI GDGCHC 3 cut(s) 4545, 4906, 5209
MlsI TGGCCA 1 cut(s) 1024
MluNI TGGCCA 1 cut(s) 1024
MlyI GAGTC 4 cut(s) 563, 1997, 2641, 6031
MmeI TCCRAC 6 cut(s) 1610, 2475, 3253, 3474, 4785, 5925
Mox20I TGGCCA 1 cut(s) 1024
Mph1103I ATGCAT 2 cut(s) 2162, 5905
MroI TCCGGA 1 cut(s) 3220
MroXI GAANNNNTTC 3 cut(s) 1428, 1687, 5164
MscI TGGCCA 1 cut(s) 1024
MslI CAYNNNNRTG 6 cut(s) 1376, 1791, 2312, 2408, 4798, 5840
Msp20I TGGCCA 1 cut(s) 1024
MspA1I CMGCKG 3 cut(s) 2936, 3904, 4760
MspCI CTTAAG 1 cut(s) 3965
MspI CCGG 6 cut(s) 984, 1892, 3176, 3221, 3509, 5561
MunI CAATTG 4 cut(s) 443, 621, 2697, 3585
Mva1269I GAATGC 4 cut(s) 2458, 2696, 4646, 4982
MvnI CGCG 5 cut(s) 40, 177, 588, 714, 3592
NciI CCSGG 3 cut(s) 984, 1892, 1893
NcoI CCATGG 3 cut(s) 1183, 2403, 2896
NdeI CATATG 3 cut(s) 966, 1336, 6279
NheI GCTAGC 1 cut(s) 2335
NlaIV GGNNCC 9 cut(s) 1383, 1414, 1773, 2437, 2803, 3407, 3909, 4188, 6355
NmeAIII GCCGAG 1 cut(s) 2899
NmuCI GTSAC 8 cut(s) 1007, 1936, 3073, 3511, 4313, 4910, 5647, 5871
NruI TCGCGA 1 cut(s) 177
NsiI ATGCAT 2 cut(s) 2162, 5905
NspI RCATGY 1 cut(s) 1878
PaeR7I CTCGAG 1 cut(s) 5555
PagI TCATGA 2 cut(s) 2794, 4659
PceI AGGCCT 3 cut(s) 3562, 3862, 4072
PciSI GCTCTTC 2 cut(s) 4149, 5355
PcsI WCGNNNNNNNCGW 3 cut(s) 44, 547, 2200
PctI GAATGC 4 cut(s) 2458, 2696, 4646, 4982
PdmI GAANNNNTTC 3 cut(s) 1428, 1687, 5164
PflMI CCANNNNNTGG 3 cut(s) 1282, 4958, 5068
PfoI TCCNGGA 1 cut(s) 2292
PinAI ACCGGT 1 cut(s) 3175
PleI GAGTC 4 cut(s) 563, 1996, 2641, 6030
PpsI GAGTC 4 cut(s) 563, 1996, 2641, 6030
Ppu21I YACGTR 1 cut(s) 1400
PpuMI RGGWCCY 1 cut(s) 4924
PshAI GACNNNNGTC 2 cut(s) 1337, 4091
PshBI ATTAAT 2 cut(s) 4002, 5163
PsiI TTATAA 2 cut(s) 2109, 5714
Psp1406I AACGTT 1 cut(s) 5299
Psp5II RGGWCCY 1 cut(s) 4924
PspFI CCCAGC 3 cut(s) 2150, 3642, 6157
PspN4I GGNNCC 9 cut(s) 1383, 1414, 1773, 2437, 2803, 3407, 3909, 4188, 6355
PspPPI RGGWCCY 1 cut(s) 4924
PspXI VCTCGAGB 1 cut(s) 5555
PstI CTGCAG 3 cut(s) 4792, 5321, 5488
PstNI CAGNNNCTG 3 cut(s) 1624, 4157, 6120
PvuII CAGCTG 2 cut(s) 3904, 4760
RruI TCGCGA 1 cut(s) 177
RseI CAYNNNNRTG 6 cut(s) 1376, 1791, 2312, 2408, 4798, 5840
SapI GCTCTTC 2 cut(s) 4149, 5355
ScaI AGTACT 1 cut(s) 2118
SchI GAGTC 4 cut(s) 563, 1997, 2641, 6031
SduI GDGCHC 3 cut(s) 4545, 4906, 5209
SexAI ACCWGGT 2 cut(s) 3789, 5097
SfcI CTRYAG 7 cut(s) 193, 1665, 4017, 4788, 5317, 5484, 6130
Sfr274I CTCGAG 1 cut(s) 5555
SinI GGWCC 9 cut(s) 205, 580, 1381, 2717, 3418, 3907, 4619, 4924, 5593
SlaI CTCGAG 1 cut(s) 5555
SmaI CCCGGG 1 cut(s) 1893
SmiMI CAYNNNNRTG 6 cut(s) 1376, 1791, 2312, 2408, 4798, 5840
SmlI CTYRAG 8 cut(s) 316, 555, 1736, 2489, 2816, 3965, 5208, 5555
SmoI CTYRAG 8 cut(s) 316, 555, 1736, 2489, 2816, 3965, 5208, 5555
SnaBI TACGTA 1 cut(s) 1400
SpeI ACTAGT 2 cut(s) 3454, 3878
SseBI AGGCCT 3 cut(s) 3562, 3862, 4072
SspI AATATT 2 cut(s) 2093, 4028
StuI AGGCCT 3 cut(s) 3562, 3862, 4072
TaiI ACGT 9 cut(s) 553, 1159, 1402, 3139, 3190, 4551, 4756, 5302, 5994
TaqII GACCGA 1 cut(s) 3812
TatI WGTACW 4 cut(s) 249, 2116, 3144, 6033
TauI GCSGC 1 cut(s) 6323
TseFI GTSAC 8 cut(s) 1007, 1936, 3073, 3511, 4313, 4910, 5647, 5871
Tsp45I GTSAC 8 cut(s) 1007, 1936, 3073, 3511, 4313, 4910, 5647, 5871
TspMI CCCGGG 1 cut(s) 1891
Van91I CCANNNNNTGG 3 cut(s) 1282, 4958, 5068
Vha464I CTTAAG 1 cut(s) 3965
VneI GTGCAC 1 cut(s) 4541
VpaK11BI GGWCC 9 cut(s) 205, 580, 1381, 2717, 3418, 3907, 4619, 4924, 5593
VspI ATTAAT 2 cut(s) 4002, 5163
XbaI TCTAGA 2 cut(s) 3742, 6244
XceI RCATGY 1 cut(s) 1878
XhoI CTCGAG 1 cut(s) 5555
XmaI CCCGGG 1 cut(s) 1891
XmaJI CCTAGG 1 cut(s) 3383
XmiI GTMKAC 1 cut(s) 2350
XmnI GAANNNNTTC 3 cut(s) 1428, 1687, 5164
ZrmI AGTACT 1 cut(s) 2118
Zsp2I ATGCAT 2 cut(s) 2162, 5905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.