Rh5AG358300

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001971
Physical Location & Seq
Forward (+)
59846050 .. 59846482
433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG358300.1

Sequence Viewer

Length: 345 bp
ATGCTTGGTGGTGGGAGAAATCTAAAAGGAGCTCAGGGGAAAATAGGTGTGAATTCATATTTTAGGAGTCATGAAGATGCCCTAACTCGCTGTCATTGGCAGATATTACAACTAGTTTCGAGTTCTGAGAGTGGCCAAATTTTGGCGTGGCTTGTGGTTGAAGGAATGATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATCAATTCTACATCACCTATAAAAGAAGAATTGAAAGAAGAATTACCAGGGAAGTATGTCTACAAGACTCTTCCTCATGGAAGACATAGTTATTACCTCTATGAGGTACTACGTACTGGTGTTAATTTCCTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

114

Amino Acids

13.01

Weight (kDa)

9.44

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 3 cut(s) 52, 138, 198
AfaI GTAC 2 cut(s) 315, 322
AfiI CCNNNNNNNGG 2 cut(s) 142, 310
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 161, 241
AhlI ACTAGT 1 cut(s) 112
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 34
AoxI GGCC 1 cut(s) 133
ApoI RAATTY 3 cut(s) 52, 138, 198
AsuHPI GGTGA 1 cut(s) 213
BalI TGGCCA 1 cut(s) 135
BanII GRGCYC 1 cut(s) 34
BarI GAAGNNNNNNTAC 2 cut(s) 251, 283
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 255
BcuI ACTAGT 1 cut(s) 112
BfaI CTAG 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 67, 159
BpiI GAAGAC 1 cut(s) 295
Bpu10I CCTNAGC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 310
Bse1I ACTGG 1 cut(s) 328
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 142, 310
BseMII CTCAG 2 cut(s) 47, 117
BseNI ACTGG 1 cut(s) 328
BshFI GGCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 34
BslI CCNNNNNNNGG 2 cut(s) 142, 310
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 34
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 46, 118
BspHI TCATGA 1 cut(s) 70
BspTI CTTAAG 1 cut(s) 173
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 33, 126
BstENI CCTNNNNNAGG 1 cut(s) 308
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstSNI TACGTA 1 cut(s) 320
BstV2I GAAGAC 1 cut(s) 295
BsuRI GGCC 1 cut(s) 135
CciI TCATGA 1 cut(s) 70
Csp6I GTAC 2 cut(s) 314, 321
CviAII CATG 2 cut(s) 71, 284
CviJI RGCY 3 cut(s) 32, 135, 151
CviKI_1 RGCY 3 cut(s) 32, 135, 151
CviQI GTAC 2 cut(s) 314, 321
DdeI CTNAG 2 cut(s) 33, 126
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 32
Eco105I TACGTA 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 32
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 34
FaeI CATG 2 cut(s) 74, 287
FaiI YATR 7 cut(s) 58, 72, 227, 264, 285, 294, 309
FatI CATG 2 cut(s) 70, 283
FblI GTMKAC 1 cut(s) 267
FriOI GRGCYC 1 cut(s) 34
FspBI CTAG 1 cut(s) 113
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 2 cut(s) 74, 287
HinfI GANTC 3 cut(s) 67, 178, 274
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 319
HpyF3I CTNAG 2 cut(s) 33, 126
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 2 cut(s) 74, 287
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 4 cut(s) 20, 240, 267, 309
LweI GCATC 2 cut(s) 67, 159
MaeI CTAG 1 cut(s) 113
MaeII ACGT 1 cut(s) 319
MboII GAAGA 5 cut(s) 86, 245, 257, 269, 300
MhlI GDGCHC 1 cut(s) 34
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 7 cut(s) 52, 138, 198, 211, 236, 248, 331
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 2 cut(s) 76, 268
MnlI CCTC 4 cut(s) 192, 291, 304, 314
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 2 cut(s) 174, 330
MslI CAYNNNNRTG 1 cut(s) 75
Msp20I TGGCCA 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
NlaIII CATG 2 cut(s) 74, 287
PagI TCATGA 1 cut(s) 70
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 142
PleI GAGTC 2 cut(s) 75, 268
PpsI GAGTC 2 cut(s) 75, 268
Ppu21I YACGTR 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
RsaI GTAC 2 cut(s) 315, 322
RsaNI GTAC 2 cut(s) 314, 321
RseI CAYNNNNRTG 1 cut(s) 75
SacI GAGCTC 1 cut(s) 34
SaqAI TTAA 2 cut(s) 174, 330
SchI GAGTC 2 cut(s) 76, 268
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 34
SetI ASST 6 cut(s) 34, 49, 226, 306, 315, 322
SfaNI GCATC 2 cut(s) 67, 159
SmiMI CAYNNNNRTG 1 cut(s) 75
SmlI CTYRAG 2 cut(s) 173, 338
SmoI CTYRAG 2 cut(s) 173, 338
SnaBI TACGTA 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 112
Sse9I AATT 7 cut(s) 52, 138, 198, 211, 236, 248, 331
SspMI CTAG 1 cut(s) 113
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 253
TaiI ACGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 119
TasI AATT 7 cut(s) 52, 138, 198, 211, 236, 248, 331
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 174, 330
Tru9I TTAA 2 cut(s) 174, 330
TspDTI ATGAA 2 cut(s) 45, 87
Van91I CCANNNNNTGG 1 cut(s) 142
Vha464I CTTAAG 1 cut(s) 173
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 3 cut(s) 52, 138, 198
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.