RchiOBHm_Chr5g0054881

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001971
Physical Location & Seq
Forward (+)
57614601 .. 57616287
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33194

Sequence Viewer

Length: 579 bp
ATGCTCATCTATGTGTTTGGACATTGGTTAATCACTTATATGACTTTTATGTCTGACAGACATGATCATGGACCTACAATTGCTGTTATTGAATGCCCAAATGTTCAGTTAATAAAGTCAGGTATACGAGCTCTTGATGATTTTCCATGTGTGAGCATTCCGTGTAATGCTCGAGACAGTCAATATCAGGTCCTTGGGTGGCAACAAGTGGCTGCAAAAATTGGCATGCAACGATGTGCTGCATCATCTCAGTGGTTGATTGATAGAATTTCTCTCTCAAGATATGCACATGTACCATTGGGCAATTTTGAAGTTGACTCCCTTATGCATACAGCTGATATCTTCTTTTCAAGAGCATTACGTGATAGCCAGCAGGTACTTTGGATATCTGATGATGGTATTCCAGATCTAGGAGGCATTAACGAAGAAGGTGGATTTTTTGCAGATGAGATACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAAAGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACATGACACTCACCATAAAATGCAATCAAAGCATTCATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

192

Amino Acids

21.48

Weight (kDa)

5.01

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1744 PF08490 21 - 143 2.2e-36 Domain of unknown function (DUF1744)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
Acc36I ACCTGC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 124
AcsI RAATTY 1 cut(s) 267
AfaI GTAC 2 cut(s) 294, 378
AfiI CCNNNNNNNGG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 4 cut(s) 92, 311, 351, 530
AluBI AGCT 2 cut(s) 131, 335
AluI AGCT 2 cut(s) 131, 335
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 171
ApeKI GCWGC 2 cut(s) 212, 239
ApoI RAATTY 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 71, 190
AsuHPI GGTGA 1 cut(s) 538
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 2 cut(s) 71, 190
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 459, 491
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 199, 226
BccI CCATC 1 cut(s) 389
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 2 cut(s) 410, 521
BfmI CTRYAG 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 364
BglII AGATCT 1 cut(s) 406
BisI GCNGC 2 cut(s) 213, 240
BlsI GCNGC 2 cut(s) 214, 241
Bme18I GGWCC 2 cut(s) 71, 190
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 71, 190
BmsI GCATC 2 cut(s) 251, 460
BpuEI CTTGAG 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 1 cut(s) 529
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse8I GATNNNNATC 1 cut(s) 529
BseDI CCNNGG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 199, 226
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 168
BsmI GAATGC 3 cut(s) 98, 156, 567
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 2 cut(s) 64, 406
BspCNI CTCAG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 364
BspQI GCTCTTC 1 cut(s) 480
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 64, 406
BssNAI GTATAC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 193
Bst1107I GTATAC 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 1 cut(s) 480
BstBAI YACGTR 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 227, 371
BstDEI CTNAG 1 cut(s) 249
BstKTI GATC 2 cut(s) 67, 409
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 2 cut(s) 64, 406
BstMWI GCNNNNNNNGC 2 cut(s) 470, 564
BstNSI RCATGY 2 cut(s) 229, 293
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 2 cut(s) 199, 226
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 257
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 2 cut(s) 227, 371
CciI TCATGA 1 cut(s) 571
Cfr13I GGNCC 2 cut(s) 71, 190
Csp6I GTAC 2 cut(s) 293, 377
CviAII CATG 7 cut(s) 62, 68, 147, 226, 290, 538, 572
CviJI RGCY 5 cut(s) 131, 212, 335, 369, 464
CviKI_1 RGCY 5 cut(s) 131, 212, 335, 369, 464
CviQI GTAC 2 cut(s) 293, 377
DdeI CTNAG 1 cut(s) 249
DpnI GATC 2 cut(s) 66, 408
DpnII GATC 2 cut(s) 64, 406
DrdI GACNNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 133
Eco32I GATATC 2 cut(s) 340, 387
Eco47I GGWCC 2 cut(s) 71, 190
Eco53kI GAGCTC 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 190
EcoRV GATATC 2 cut(s) 340, 387
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 7 cut(s) 65, 71, 150, 229, 293, 541, 575
FatI CATG 7 cut(s) 61, 67, 146, 225, 289, 537, 571
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 1 cut(s) 124
Fnu4HI GCNGC 2 cut(s) 213, 240
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 213, 240
FspBI CTAG 2 cut(s) 410, 521
GluI GCNGC 2 cut(s) 213, 240
Hin1II CATG 7 cut(s) 65, 71, 150, 229, 293, 541, 575
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 317, 530
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 2 cut(s) 125, 316
Hpy188I TCNGA 4 cut(s) 55, 391, 480, 535
Hpy188III TCNNGA 6 cut(s) 134, 173, 279, 351, 404, 572
Hpy8I GTNNAC 2 cut(s) 125, 316
HpyAV CCTTC 2 cut(s) 422, 500
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 7 cut(s) 215, 229, 242, 287, 328, 443, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 564
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 7 cut(s) 65, 71, 150, 229, 293, 541, 575
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 2 cut(s) 64, 406
LguI GCTCTTC 1 cut(s) 480
LpnPI CCDG 6 cut(s) 105, 173, 359, 383, 417, 446
Lsp1109I GCAGC 2 cut(s) 199, 226
LweI GCATC 2 cut(s) 251, 460
MaeI CTAG 2 cut(s) 410, 521
MaeII ACGT 1 cut(s) 361
MalI GATC 2 cut(s) 66, 408
MboI GATC 2 cut(s) 64, 406
MboII GAAGA 3 cut(s) 334, 437, 467
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 4 cut(s) 78, 219, 267, 304
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 2 cut(s) 407, 503
Mph1103I ATGCAT 1 cut(s) 330
MseI TTAA 3 cut(s) 29, 110, 420
MslI CAYNNNNRTG 4 cut(s) 11, 38, 66, 250
MspA1I CMGCKG 1 cut(s) 335
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 3 cut(s) 98, 156, 567
MwoI GCNNNNNNNGC 2 cut(s) 470, 564
NdeII GATC 2 cut(s) 64, 406
NlaIII CATG 7 cut(s) 65, 71, 150, 229, 293, 541, 575
NsiI ATGCAT 1 cut(s) 330
NspI RCATGY 2 cut(s) 229, 293
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 171
PagI TCATGA 1 cut(s) 571
PciI ACATGT 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 480
PctI GAATGC 3 cut(s) 98, 156, 567
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 2 cut(s) 214, 241
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 190
PscI ACATGT 1 cut(s) 289
Psp124BI GAGCTC 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 190
PspPI GGNCC 2 cut(s) 71, 190
PspPPI RGGWCCY 1 cut(s) 190
PsuI RGATCY 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 294, 378
RsaNI GTAC 2 cut(s) 293, 377
RseI CAYNNNNRTG 4 cut(s) 11, 38, 66, 250
SacI GAGCTC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 3 cut(s) 29, 110, 420
SatI GCNGC 2 cut(s) 213, 240
Sau3AI GATC 2 cut(s) 64, 406
Sau96I GGNCC 2 cut(s) 71, 190
SchI GAGTC 1 cut(s) 311
SduI GDGCHC 1 cut(s) 133
SetI ASST 9 cut(s) 76, 124, 133, 192, 337, 364, 378, 433, 514
SfaNI GCATC 2 cut(s) 251, 460
SfcI CTRYAG 1 cut(s) 465
Sfr274I CTCGAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 71, 190
SlaI CTCGAG 1 cut(s) 171
SmiMI CAYNNNNRTG 4 cut(s) 11, 38, 66, 250
SmlI CTYRAG 2 cut(s) 171, 277
SmoI CTYRAG 2 cut(s) 171, 277
SphI GCATGC 1 cut(s) 229
Sse9I AATT 4 cut(s) 78, 219, 267, 304
SspMI CTAG 2 cut(s) 410, 521
SstI GAGCTC 1 cut(s) 133
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 364
TaqI TCGA 1 cut(s) 172
TasI AATT 4 cut(s) 78, 219, 267, 304
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 3 cut(s) 29, 110, 420
Tru9I TTAA 3 cut(s) 29, 110, 420
TscAI CASTG 1 cut(s) 257
TseI GCWGC 2 cut(s) 212, 239
TspDTI ATGAA 1 cut(s) 560
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 71, 190
XapI RAATTY 1 cut(s) 267
XceI RCATGY 2 cut(s) 229, 293
XhoI CTCGAG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 124
XspI CTAG 2 cut(s) 410, 521
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.