Rh5BG369700

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001971
Physical Location & Seq
Forward (+)
58038605 .. 58060965
22361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG369700.1

Sequence Viewer

Length: 6645 bp
ATGAGCGGCTACGAGAGCCGGAAACGGGACCGGAGAGACTCGTGGAACTCCAAGAAGCAGCAGAAGCTCACGCGCACCGCCGACGAAGACCTCGAGTCAAAGCTCGGACTCGATCTATTCTCCGAAGGTGAAACTAGGCTCGGCTGGTTACTCACTTTCGCAACCTCATCCTGTGAAGATCAAGACACCCATAAAGTTTATAGCTGCGTTGATCTGTATTTCGTTACACAGGATGGCTCCGCTTTCAAGTCAAAATACAAGTTTCGCCCTTATTTCTATGCAGCCACAAAGGAAAAAATGGAAATGGATGTTGAAGCATATTTGAGACGGCGCTATGAAAGTCGTATTGCTGATATCGAAATAGTGGCGAAAGAAGATCTTGATCTTAAAAACCATTTGTCTGGCCTACACAAGTCCTATTTGAAGTTATCATTTGATACTGTTCAACAATTAATGAATGTCAAGAGTGACCTATTGCAAGTCGTCGAAAGAAACCACACAAAATTTGATGCTGCCGAAGCTTATGAGTCAATACTGCTAGGGAAACGAGAACAAAGGCCTCAAGATTTCCTAGATTGTATTGTTGATCTTCGTGAATACGATGTTCCCTACCATGTTCGCTTCGCTATTGACAATGATGTGCGATGTGGCCAGTGGTACAATGTTAGTGTATCCAGTACGGGTGTTATGCTTGAGAGGAGGACAGATCTTCTGCAGCATGCTGAAGTTCATGTTTGTGCTTTTGATATTGAGACAACTAAGCTGCCCTTAAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGACGGACAAGGTTACCTGATTATTAATAGAGAGTGTGTTGGAGCTGATATAGAGTATTTGGAGTACACTCCAAAAGAAGAATTTGTAGGGCATTTCAAGGTGACAAATGTGGAAAATGAGAGAGAGCTTCTAAGACATTGGTTTGCCCATATGCGGGAGGTGAAGCCTGGAATCTATGTCACATACAATGGGGATTTTTTCGATTGGCCTTTCCTGGAAACAAGAGCTGCACATCATGGGTTCAAAATGAATGATGAGGTTGGATTTTTATGTGACAAGAATCAGGGGGAATGTAGAGCTAAATTTGCTTGTCATCTTGATTGCTTTGCTTGGGTCAAACGTGATAGTTATCTGCCCCAAGGGAGCCAGGGCCTTAAGGCTGTTACGAAGGCAAAGTTGGGTTATGATCCAATAGAGGTGAATCCTGAGGATATGGTTCGCTTTGCGAAAGAAGAACCTCAGAGGATGGCATCCTATTCTGTTTCTGATGCTGTTGCAACCTATTATTTGTACATGACCTATGTTCATCCATTTATCTTCTCTCTTGCAACTATTATTCCAATGTCTCCGGATGAGGTTTTGCGCAAGGGTAGTGGAACCCTTTGTGAAATGCTTTTAATGGTCCAGGCATACAAGGCAAATGTCATCTGTCCTAACAAGCACCAATCTGACCCCGAAAAGTTCTATAAAAACCACCTTTTGGAAAGTGAGACTTACATAGGTGGCCATGTGGAATGCCTTGAAAGTGGTGTTTTCAGATCTGATCTTCCAACTAATTTTAGGCTTGATCCAGCTGCTTATGAGCAATTGATCAACAACCTTGATCGAGATCTTCAATATGCTATCAGAGTAGAGGGAAAGATGGACTTGGATTCTGTGTCCAATTATGATGAAGTGAAGAGTTCTATTCTGGAAAAGCTTGTGAGGTTGCGAGATGAACCGATACGAGAAGAATGCCCACTTATTTATCACCTGGATGTAGCTGCAATGTATCCCAACATTATTTTAACAAACAGGCTTCAGCCCACATCAATTGTTACAGATGAGGTCTGCACTGCTTGTGATTTCAACCGTCCAGGAAAGACATGTCTACGAAAGCTGGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAAAAGAAGCGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTCTGTTGACGGTGCCACTTCCAAGCAGTCAAGGTCTTTTCTTGAATTGCCTAAAATGGAACAACAAACAAGGCTCAAAGAACGTCTAAAGAAATACTGTCAGAAGGCATATAAACGCGTAGTAGACAAGCCAGTCACTGAACTTCGGGAAGCAGAGATTTGCATGAGGGAAAATTCATTCTATATTGACACAGTTCGCAGCTTTCGAGATAGAAGGTACGAGTATAAAGGGCTTAATAAGGTTTGGAAGGGCAAGTTGTCTGAAGCAAAGGCTAGTAAAAATTCTATAAAAATCCAGGAAGCACAAAATATGGTGGTGCTTTATGATTCCCTACAACTTGCACATAAGTGTATACTTAACTCCTTTTATGGATACGTCATGCGCAAGGGTGCAAGATGGTATTCAATGGAAATGGCTGGAGTAGTTACATATACTGGAGCTAAAATTATTCAGAATGCTCGGTTGCTAGTTGAAAAAATTGGAAAGCCTCTCGAGTTAGATACAGATGGTATCTGGTGTGTGCTTCCTGGATCTTTTCCGGAGAACTTTACTTTCAAAACAAAAGACTTGAAGAAGAAGCTAACTATCTCATATCCATGCGTCATGCTTAATGTTGATGTGGCGAGAAACAATACAAACGATCAATACCAGACTCTTACAGATCCGATCGAGAAAACTTATGCAACTCATAGTGAATGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTATTCCTGCTTCTAAGGAAGAAGGAATTCTAATTAAGAAGCGCTATGCTGTCTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTTGAGATCAAACGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAGCTTTTTGATAAGTTCCTTCACGGTTCTACATTAGAGGAATGCTATTCAGCAGTTGCTTCTGTTGCCAACCGCTGGCTTGATCTTCTTGATAATCAAGGAAAGGATATAGCAGACAGTGAGTTGCTTGATTATATATCAGAATCAAGCACCATGAGCAAGTCTTTGGCGGAATATGGAAGCCAAAAGTCATGTGCAGTAACCACTGCAAGGCGTCTTGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTACGTTGCCAGTATGTAGTTGCATGTGAACCAAAGGGAACACCTGTCAGTGAGCGTGCTGTTCCAGTTGCAATATTCGAAACAGATGATGAAATTATGAAGTTTTATGTACGGAAGTGGTGCAAGGTCTCCGATGTTGACATCAGATCCATTATAGACTGGTCCTATTACAAACAACGTCTAGGTTCAGCAATTCAGAAAATCGTCACAATTCCCGCTGCAATGCAAAAGGTTGCAAATCCTGTCCCTAGGGTTGTCCATCCTGATTGGCTACATAAGAAGGTTCGTGAGAAGGAAGATAAATTTCGACAACGCAAATTGGTTGATATCTTCAGGTCATTGAATGGGGATCATTTGGTGCAAAACAATGATGCTATTGGCTCTAATCATGTGGTAGATGAAGATATTGTTGAAGATTTGGAAGACTTCAAATGCAAAAGCAGTTCCATAAGTAGACCGAGACCAATTGTTCACTGTTATGAAGTTAATAACCAGAAAAAATCAGTCAAGGCAAATGGTAAAGGAGGTCCAGAGCAACAACAAACTAATCATATAGAAAATGCTCATCAAGAGCAAACTGCTGTCACTCGGGAAGAAATTGACAAAAATGTAGATTATCAAGGATGGCTTGCAATAAAGAAAAGAAAGTGGAAAGATACTCTTGACAAGAGGAAGAAACAACGATTGAATAATGCAAGGTCTGCTCATAATGCTAATGACAAGATGCTTGGTGGTGGGAGAAATCTCAAAGGGGCTCAGGGGAAAATAGGTGTGAATTCATATTTTAGGAGTCATGAAGATGCCCTAACTCGCTGTCATTGGCAGATATTACAACTAGTTCCGAGTTCTGAGAGTGGCCAAATTTTGGCGTGGCTTGTTGTTGAAGGAATGATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATCAATTCTACATCACCTATAAAAGAAGAATTGAAAGAAGAATTACCAGGGAAGTATGTCAACAAGACTCTTCCTCATGGAAGACATAGTTATTACCTATATGAGGTTTCAGTTGATGAGAAGAGGTTTAGGACACTGAGAAAAAAGCTTGCGGCTCTTATCGCTGATCCAGATATTGAGGGAATATATGAAACAAAGGTGCCTTCGGAGTTTAATGTAATCACTCAGATTGGTTGTGTCTGTAAATTGGACAAAAAAGCTCAGGGAGATGCCAAAAATGGCTGGAGTGTTAGTGCACTGGACATGAAGAATACGACAGAGTGCTCTTATCTGGCCCAATCCATCGCTTTCTTTTATTTGTATCACAGCATCTCTGAAGGGCGCGCAATATTTGTTGCCTATTTTCCTGCATCAAGAACAATAATTGTTGTGGTGGTGAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAAAAACAATTCCGCGACGCTTGCCAGGCATTATCACTTCAGCTGCCTCCTAGGAATGGAATCATTATCAAGGTAGACTACGTTGGATATGTAAAGGATGCTGAAAAGATTTTGCACCAAGCAATTAATGAGCACAGACATGATCATGGACCTACAATTGCTGTTATTGAATGCCCAAATGTTCAGTTAATGAAGTCAGGTATACGAGCTCTTGATGATTTTCCATGTGTGAGCATTCCGTGTAATGCTCGAGACAGTCAATATCAGGTCCTTGGGTGGCAACAAGTGGCTGCAAAAATTGGCATGCAACGATGTGCTGCATCATCTCAGTGGTTGATTGATAGAATTTCTCTCTCAAGATATGCACATGTACCATTGGGCAATTTTGAAGTTGACTCCCTTATGCATACAGCTGATATCTTCTTTTCAAGAGCATTACGTGATAGCCAGCAGGTACTTTGGATATCTGATGATGGTACTCCAGATCTAGGAGGCATTAATGAAGAAGGTGGATTTTTTGCAGATGAGGTTCATCAACCATTTGTAATTACTAAACCTGGAGCATATAGAAAGGTCACTGTGGAGCTAAAGATACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAATGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACATGAACTCCGGATCACATACTTCTAATATGCAGGCTGCCTTTGATGAAGCTACATCATCTGCACCTGCATTCCGTGTTTTGAAACAGTTGATCCACCGGTGTCTTGCAGATGCACATACATCTGGAAATGTGTATGCTGATGCACTACTGCAACATCTGTATCGATGGCTTTGCAGCCCTCAGTCTAAACTTCATGACCCAGCACTTCAACGCCTACTTCACAAGGTCATGAAAAAGGTGCTTTTGATTTTATTGGATGAGTTCCGCAAATTGGGTGCAAATATCATATTTGCAAATTTCTATAAGGTTATTATAGACACCGGGAAATTTGATGTATCCGCAGCCAAAGCTTATTGTGATAGTGTGCTTAAAACTCTCCAAAAAAGAGAACTATTTGAGTGGATTGAGCTTGAACCATTGCACTACTGGCATTCTTTGCTTTTCATGGATCAGTATAATTATGGTGGAATTCCGGTTAGAGCTGATGAAAGCATGCATGGTGAATCTCAAGTGGATATTGTATCGAGCTGGAACATTGCTGAATACTTACCCAAGAAAATTCAGGACCATTTTATTTTTATTGTTTCTCAGTTTTTGTATATTCCCTGGAATTATGCACAGAAGCAGTCGGCCGTTAGAGCGACTTTACAGGATGGCAGCAGTTGCACACCATCAATTACGGTTGCAGCTGCTGAGACTTTGGAATCTCACATGACCGATTTTCTTAAAGGACAGATCAGCTCGTACTTTACTGAAAAGCTTCTGGGAATTGTTCGTGATACAATACTTCATATGAAGGAACTGAACAAGTCCGAGAATGACTATCGGACATCTCCTGGACTGCCACAACTTGCTGGTAATATCCACAAAGGAGATGCGGCACTTGAGTTCATTAAGAATGTCTGCGGTGTTTTGCTCATTGACCAAAGTGTTGAGCACGACGTTATGGTAATGAGAAGTAATCTATTAAAATATATGCATGTGAGAGAGTCTGCTCCTGAAGCTCAGTTTTGCGATCCCTGCCCATCCTTGTTCTTACGAAATGTCATTTGCAGCTACTGCAATGACTGTAGAGACCTAGACATCTGCCGTGATTCAGCTCTACTAGCTGGGGAGTGGCGCTGTGCTGTGCCCCAATGCGGGCAGCCTTATGACAGAGAGCTTATGGAGAATACCCTTCTTCAGATTGTACAGCAGAGAGAGCGTCTCTACCAGCTACAGGATCTGGTGTGTCTCAGGTGTAACCAAGTGAAAGCTGCACATTTAGCAGAACAATGTGGATGTGCCGGCTCTTTTAGCTGTAAGGAAGACGCAGCTGAATTTTGTGAAAAGATGCAGTTAATCTTGAAAATTGCAATTCATCAGAAATTTCAATTTCTACAAGAGTGCACTGAGTGGATTTTAGAAGTTGAGAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2214

Amino Acids

253.64

Weight (kDa)

6.46

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 196 - 402 4.1e-56 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 749 - 1076 7.8e-19 DNA polymerase family B
POL2_thumb PF22634 948 - 1122 4.2e-78 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1532 - 1898 3.4e-114 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2075 - 2141 1.5e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2146 - 2203 5.3e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5296
AasI GACNNNNNNGTC 1 cut(s) 2132
Acc16I TGCGCA 2 cut(s) 1406, 2384
Acc36I ACCTGC 2 cut(s) 4987, 5296
AccB1I GGYRCC 2 cut(s) 2012, 4267
AccB7I CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
AccII CGCG 4 cut(s) 73, 2118, 4452, 4560
AccIII TCCGGA 3 cut(s) 1390, 2539, 5231
AcoI YGGCCR 4 cut(s) 649, 1546, 3994, 5853
AcuI CTGAAG 9 cut(s) 744, 1826, 2283, 2876, 3454, 4464, 4568, 6243, 6389
AcyI GRCGYC 1 cut(s) 3094
AdeI CACNNNGTG 1 cut(s) 6618
AfeI AGCGCT 1 cut(s) 2783
AflII CTTAAG 2 cut(s) 1196, 4034
AflIII ACRYGT 3 cut(s) 1907, 2116, 4912
AgeI ACCGGT 1 cut(s) 5319
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 3973
AjuI GAANNNNNNNTTGG 2 cut(s) 1202, 1234
AleI CACNNNNGTG 1 cut(s) 2347
Alw21I GWGCWC 6 cut(s) 4366, 4394, 4680, 4756, 6162, 6614
Alw44I GTGCAC 2 cut(s) 4362, 6610
AlwNI CAGNNNCTG 5 cut(s) 785, 2138, 5915, 6282, 6448
Ama87I CYCGRG 4 cut(s) 92, 2492, 3726, 4794
Aor13HI TCCGGA 3 cut(s) 1390, 2539, 5231
Aor51HI AGCGCT 1 cut(s) 2783
AoxI GGCC 9 cut(s) 403, 557, 649, 1028, 1192, 1546, 3994, 4401, 5853
ApaLI GTGCAC 2 cut(s) 4362, 6610
AseI ATTAAT 4 cut(s) 452, 846, 4671, 5043
AsiGI ACCGGT 1 cut(s) 5319
Asp700I GAANNNNTTC 2 cut(s) 2766, 5982
AspA2I CCTAGG 2 cut(s) 3386, 4595
AspLEI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
AsuC2I CCSGG 1 cut(s) 5545
AsuII TTCGAA 1 cut(s) 3216
AvaI CYCGRG 4 cut(s) 92, 2492, 3726, 4794
AvaII GGWCC 9 cut(s) 28, 1444, 2723, 2808, 3300, 3665, 4694, 4813, 5788
AvrII CCTAGG 2 cut(s) 3386, 4595
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 3 cut(s) 4366, 6357, 6614
BaeI ACNNNNGTAYC 4 cut(s) 3108, 3141, 5366, 5399
BalI TGGCCA 3 cut(s) 651, 1548, 3996
BanI GGYRCC 2 cut(s) 2012, 4267
BanII GRGCYC 2 cut(s) 3895, 4756
BarI GAAGNNNNNNTAC 2 cut(s) 5145, 5177
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 4 cut(s) 93, 3567, 4156, 6537
Bbv12I GWGCWC 6 cut(s) 4366, 4394, 4680, 4756, 6162, 6614
BceAI ACGGC 3 cut(s) 344, 5840, 6297
BcgI CGANNNNNNTGC 4 cut(s) 1758, 1792, 5376, 5410
BciVI GTATCC 4 cut(s) 682, 1824, 2366, 5569
BclI TGATCA 2 cut(s) 1632, 4687
BcnI CCSGG 1 cut(s) 5545
BcuI ACTAGT 1 cut(s) 3973
BfmI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
BfoI RGCGCY 3 cut(s) 334, 2785, 6346
BfrI CTTAAG 2 cut(s) 1196, 4034
BfuAI ACCTGC 2 cut(s) 4987, 5296
BfuI GTATCC 4 cut(s) 682, 1824, 2366, 5569
BglII AGATCT 5 cut(s) 376, 706, 1580, 1651, 5029
BlnI CCTAGG 2 cut(s) 3386, 4595
BlpI GCTNAGC 1 cut(s) 2880
Bme18I GGWCC 9 cut(s) 28, 1444, 2723, 2808, 3300, 3665, 4694, 4813, 5788
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 4 cut(s) 92, 2492, 3726, 4794
BmiI GGNNCC 8 cut(s) 29, 238, 1187, 1420, 2014, 2809, 2810, 4269
BpiI GAAGAC 4 cut(s) 93, 3567, 4156, 6537
BplI GAGNNNNNCTC 2 cut(s) 6414, 6446
BpmI CTGGAG 5 cut(s) 2439, 2457, 4372, 5010, 5124
Bpu10I CCTNAGC 2 cut(s) 3894, 4329
Bpu1102I GCTNAGC 1 cut(s) 2880
Bpu14I TTCGAA 1 cut(s) 3216
BpuEI CTTGAG 5 cut(s) 546, 713, 4885, 5715, 6128
BpuMI CCSGG 1 cut(s) 5545
Bsa29I ATCGAT 1 cut(s) 5386
BsaAI YACGTR 1 cut(s) 4985
BsaBI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BsaHI GRCGYC 1 cut(s) 3094
BsaI GGTCTC 3 cut(s) 3271, 3592, 6291
BsaJI CCNNGG 7 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828
BsaWI WCCGGW 6 cut(s) 30, 1390, 2539, 5231, 5319, 5695
BsaXI ACNNNNNCTCC 4 cut(s) 4345, 4375, 5213, 5243
Bse118I RCCGGY 2 cut(s) 5319, 6509
Bse1I ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 3302, 4371, 5654
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
Bse8I GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BseAI TCCGGA 3 cut(s) 1390, 2539, 5231
BseCI ATCGAT 1 cut(s) 5386
BseDI CCNNGG 7 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828
BseJI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BseMI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
BseNI ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 3302, 4371, 5654
BsePI GCGCGC 1 cut(s) 4450
BseRI GAGGAG 1 cut(s) 712
BseSI GKGCMC 3 cut(s) 4366, 6357, 6614
BseX3I CGGCCG 1 cut(s) 5853
BseYI CCCAGC 2 cut(s) 5422, 6332
BsgI GTGCAG 5 cut(s) 1035, 1858, 3096, 5268, 6465
Bsh1236I CGCG 4 cut(s) 73, 2118, 4452, 4560
Bsh1285I CGRYCG 2 cut(s) 2670, 5856
BshFI GGCC 9 cut(s) 405, 559, 651, 1030, 1194, 1548, 3996, 4403, 5855
BshNI GGYRCC 2 cut(s) 2012, 4267
BshTI ACCGGT 1 cut(s) 5319
BshVI ATCGAT 1 cut(s) 5386
BsiEI CGRYCG 2 cut(s) 2670, 5856
BsiHKAI GWGCWC 6 cut(s) 4366, 4394, 4680, 4756, 6162, 6614
BsiHKCI CYCGRG 4 cut(s) 92, 2492, 3726, 4794
BsiSI CCGG 9 cut(s) 19, 31, 1391, 2540, 5232, 5320, 5544, 5696, 6510
BslFI GGGAC 3 cut(s) 41, 2821, 3368
BsmBI CGTCTC 2 cut(s) 319, 6434
BsmFI GGGAC 3 cut(s) 41, 2821, 3368
BsmI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
BsnI GGCC 9 cut(s) 405, 559, 651, 1030, 1194, 1548, 3996, 4403, 5855
Bso31I GGTCTC 3 cut(s) 3271, 3592, 6291
BsoBI CYCGRG 4 cut(s) 92, 2492, 3726, 4794
Bsp119I TTCGAA 1 cut(s) 3216
Bsp1286I GDGCHC 8 cut(s) 3895, 4366, 4394, 4680, 4756, 6162, 6357, 6614
Bsp13I TCCGGA 3 cut(s) 1390, 2539, 5231
Bsp1407I TGTACA 2 cut(s) 1332, 6412
Bsp1720I GCTNAGC 1 cut(s) 2880
BspANI GGCC 9 cut(s) 405, 559, 651, 1030, 1194, 1548, 3996, 4403, 5855
BspDI ATCGAT 1 cut(s) 5386
BspEI TCCGGA 3 cut(s) 1390, 2539, 5231
BspFNI CGCG 4 cut(s) 73, 2118, 4452, 4560
BspHI TCATGA 3 cut(s) 3931, 5416, 5451
BspLI GGNNCC 8 cut(s) 29, 238, 1187, 1420, 2014, 2809, 2810, 4269
BspMAI CTGCAG 1 cut(s) 717
BspMI ACCTGC 2 cut(s) 4987, 5296
BspQI GCTCTTC 1 cut(s) 5166
BspT104I TTCGAA 1 cut(s) 3216
BspT107I GGYRCC 2 cut(s) 2012, 4267
BspTI CTTAAG 2 cut(s) 1196, 4034
BspTNI GGTCTC 3 cut(s) 3271, 3592, 6291
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
BsrFI RCCGGY 2 cut(s) 5319, 6509
BsrGI TGTACA 2 cut(s) 1332, 6412
BsrI ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 3302, 4371, 5654
BssAI RCCGGY 2 cut(s) 5319, 6509
BssECI CCNNGG 7 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828
BssHII GCGCGC 1 cut(s) 4450
BssNAI GTATAC 2 cut(s) 2354, 4748
BssNI GRCGYC 1 cut(s) 3094
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Bst1107I GTATAC 2 cut(s) 2354, 4748
Bst2BI CACGAG 1 cut(s) 40
Bst6I CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
BstACI GRCGYC 1 cut(s) 3094
BstAFI CTTAAG 2 cut(s) 1196, 4034
BstAPI GCANNNNNTGC 4 cut(s) 3839, 5659, 5886, 6282
BstAUI TGTACA 2 cut(s) 1332, 6412
BstBAI YACGTR 1 cut(s) 4985
BstBI TTCGAA 1 cut(s) 3216
BstEII GGTNACC 1 cut(s) 833
BstENI CCTNNNNNAGG 1 cut(s) 4169
BstFNI CGCG 4 cut(s) 73, 2118, 4452, 4560
BstH2I RGCGCY 3 cut(s) 334, 2785, 6346
BstHHI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
BstMCI CGRYCG 2 cut(s) 2670, 5856
BstNSI RCATGY 7 cut(s) 722, 1911, 3165, 4852, 4916, 5719, 6206
BstPI GGTNACC 1 cut(s) 833
BstSFI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
BstSLI GKGCMC 3 cut(s) 4366, 6357, 6614
BstUI CGCG 4 cut(s) 73, 2118, 4452, 4560
BstV2I GAAGAC 4 cut(s) 93, 3567, 4156, 6537
BstX2I RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5029, 6445
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5029, 6445
BstZ17I GTATAC 2 cut(s) 2354, 4748
BstZI CGGCCG 1 cut(s) 5853
Bsu15I ATCGAT 1 cut(s) 5386
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 4 cut(s) 682, 1824, 2366, 5569
BsuRI GGCC 9 cut(s) 405, 559, 651, 1030, 1194, 1548, 3996, 4403, 5855
BsuTUI ATCGAT 1 cut(s) 5386
BtgZI GCGATG 2 cut(s) 658, 4396
BtsI GCAGTG 2 cut(s) 1875, 3084
BveI ACCTGC 2 cut(s) 4987, 5296
CaiI CAGNNNCTG 5 cut(s) 785, 2138, 5915, 6282, 6448
CciI TCATGA 3 cut(s) 3931, 5416, 5451
CfoI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
Cfr10I RCCGGY 2 cut(s) 5319, 6509
ClaI ATCGAT 1 cut(s) 5386
CseI GACGC 5 cut(s) 2590, 3083, 4571, 6416, 6542
CspAI ACCGGT 1 cut(s) 5319
CspCI CAANNNNNGTGG 4 cut(s) 1396, 1431, 1837, 1872
DraIII CACNNNGTG 1 cut(s) 6618
DrdI GACNNNNNNGTC 1 cut(s) 2132
DriI GACNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 2132
EaeI YGGCCR 4 cut(s) 649, 1546, 3994, 5853
EagI CGGCCG 1 cut(s) 5853
Eam1104I CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
EciI GGCGGA 1 cut(s) 3065
Ecl136II GAGCTC 1 cut(s) 4754
EclXI CGGCCG 1 cut(s) 5853
Eco130I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 2 cut(s) 3895, 4756
Eco31I GGTCTC 3 cut(s) 3271, 3592, 6291
Eco32I GATATC 4 cut(s) 355, 3466, 4963, 5010
Eco47I GGWCC 9 cut(s) 28, 1444, 2723, 2808, 3300, 3665, 4694, 4813, 5788
Eco47III AGCGCT 1 cut(s) 2783
Eco52I CGGCCG 1 cut(s) 5853
Eco53kI GAGCTC 1 cut(s) 4754
Eco57I CTGAAG 9 cut(s) 744, 1826, 2283, 2876, 3454, 4464, 4568, 6243, 6389
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 4 cut(s) 92, 2492, 3726, 4794
Eco91I GGTNACC 1 cut(s) 833
EcoICRI GAGCTC 1 cut(s) 4754
EcoNI CCTNNNNNAGG 1 cut(s) 4169
EcoO109I RGGNCCY 3 cut(s) 1192, 2808, 4813
EcoO65I GGTNACC 1 cut(s) 833
EcoRI GAATTC 3 cut(s) 2766, 3913, 5691
EcoRV GATATC 4 cut(s) 355, 3466, 4963, 5010
EcoT14I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
EcoT22I ATGCAT 3 cut(s) 4953, 5721, 6204
EcoT38I GRGCYC 2 cut(s) 3895, 4756
ErhI CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Esp3I CGTCTC 2 cut(s) 319, 6434
FaqI GGGAC 3 cut(s) 41, 2821, 3368
FauI CCCGC 3 cut(s) 969, 3361, 6356
FauNDI CATATG 2 cut(s) 972, 6015
FbaI TGATCA 2 cut(s) 1632, 4687
FblI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
FriOI GRGCYC 2 cut(s) 3895, 4756
FspI TGCGCA 2 cut(s) 1406, 2384
GlaI GCGC 8 cut(s) 74, 332, 1406, 2384, 2783, 4451, 4453, 6344
GsaI CCCAGC 2 cut(s) 5426, 6336
GsuI CTGGAG 5 cut(s) 2439, 2457, 4372, 5010, 5124
HaeII RGCGCY 3 cut(s) 334, 2785, 6346
HaeIII GGCC 9 cut(s) 405, 559, 651, 1030, 1194, 1548, 3996, 4403, 5855
HapII CCGG 9 cut(s) 19, 31, 1391, 2540, 5232, 5320, 5544, 5696, 6510
HgaI GACGC 5 cut(s) 2590, 3083, 4571, 6416, 6542
HhaI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
Hin1I GRCGYC 1 cut(s) 3094
Hin6I GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
HinP1I GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
HincII GTYRAC 5 cut(s) 823, 2008, 3277, 4129, 4939
HindII GTYRAC 5 cut(s) 823, 2008, 3277, 4129, 4939
HindIII AAGCTT 5 cut(s) 519, 1739, 4214, 5571, 5981
HpaII CCGG 9 cut(s) 19, 31, 1391, 2540, 5232, 5320, 5544, 5696, 6510
Hpy99I CGWCG 4 cut(s) 86, 488, 4565, 6167
HpyCH4IV ACGT 9 cut(s) 1162, 2083, 2376, 2842, 3142, 3316, 4626, 4984, 6165
HpySE526I ACGT 9 cut(s) 1162, 2083, 2376, 2842, 3142, 3316, 4626, 4984, 6165
Hsp92I GRCGYC 1 cut(s) 3094
HspAI GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
KflI GGGWCCC 1 cut(s) 2808
Kpn2I TCCGGA 3 cut(s) 1390, 2539, 5231
KroI GCCGGC 1 cut(s) 6509
KroNI GCCGGC 1 cut(s) 6511
Ksp22I TGATCA 2 cut(s) 1632, 4687
LguI GCTCTTC 1 cut(s) 5166
LmnI GCTCC 7 cut(s) 242, 863, 1185, 2438, 5105, 5128, 6223
MaeII ACGT 9 cut(s) 1162, 2083, 2376, 2842, 3142, 3316, 4626, 4984, 6165
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 5 cut(s) 1628, 1854, 2703, 3603, 4701
MflI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5029, 6445
MhlI GDGCHC 8 cut(s) 3895, 4366, 4394, 4680, 4756, 6162, 6357, 6614
MlsI TGGCCA 3 cut(s) 651, 1548, 3996
MluI ACGCGT 1 cut(s) 2116
MluNI TGGCCA 3 cut(s) 651, 1548, 3996
MmeI TCCRAC 4 cut(s) 841, 1063, 1616, 4609
Mox20I TGGCCA 3 cut(s) 651, 1548, 3996
Mph1103I ATGCAT 3 cut(s) 4953, 5721, 6204
MroI TCCGGA 3 cut(s) 1390, 2539, 5231
MroNI GCCGGC 1 cut(s) 6509
MroXI GAANNNNTTC 2 cut(s) 2766, 5982
MscI TGGCCA 3 cut(s) 651, 1548, 3996
MslI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4683, 4689, 4873
Msp20I TGGCCA 3 cut(s) 651, 1548, 3996
MspA1I CMGCKG 7 cut(s) 1616, 2955, 3356, 4588, 4958, 5912, 6539
MspCI CTTAAG 2 cut(s) 1196, 4034
MspI CCGG 9 cut(s) 19, 31, 1391, 2540, 5232, 5320, 5544, 5696, 6510
MunI CAATTG 5 cut(s) 1628, 1854, 2703, 3603, 4701
Mva1269I GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
MvnI CGCG 4 cut(s) 73, 2118, 4452, 4560
NaeI GCCGGC 1 cut(s) 6511
NciI CCSGG 1 cut(s) 5545
NdeI CATATG 2 cut(s) 972, 6015
NgoMIV GCCGGC 1 cut(s) 6509
NlaIV GGNNCC 8 cut(s) 29, 238, 1187, 1420, 2014, 2809, 2810, 4269
NmeAIII GCCGAG 1 cut(s) 120
NmuCI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
NsbI TGCGCA 2 cut(s) 1406, 2384
NsiI ATGCAT 3 cut(s) 4953, 5721, 6204
NspI RCATGY 7 cut(s) 722, 1911, 3165, 4852, 4916, 5719, 6206
NspV TTCGAA 1 cut(s) 3216
OliI CACNNNNGTG 1 cut(s) 2347
PaeI GCATGC 3 cut(s) 722, 4852, 5719
PaeR7I CTCGAG 3 cut(s) 92, 2492, 4794
PagI TCATGA 3 cut(s) 3931, 5416, 5451
PaqCI CACCTGC 1 cut(s) 5296
PauI GCGCGC 1 cut(s) 4450
PceI AGGCCT 1 cut(s) 559
PciI ACATGT 2 cut(s) 1907, 4912
PciSI GCTCTTC 1 cut(s) 5166
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 2203
PctI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
PdiI GCCGGC 1 cut(s) 6511
PdmI GAANNNNTTC 2 cut(s) 2766, 5982
PflMI CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
PfoI TCCNGGA 5 cut(s) 1035, 1897, 2295, 2527, 6058
PinAI ACCGGT 1 cut(s) 5319
Ple19I CGATCG 1 cut(s) 2670
Ppu21I YACGTR 1 cut(s) 4985
PpuMI RGGWCCY 2 cut(s) 2808, 4813
PscI ACATGT 2 cut(s) 1907, 4912
PshBI ATTAAT 4 cut(s) 452, 846, 4671, 5043
Psp124BI GAGCTC 1 cut(s) 4756
Psp5II RGGWCCY 2 cut(s) 2808, 4813
PspEI GGTNACC 1 cut(s) 833
PspFI CCCAGC 2 cut(s) 5422, 6332
PspN4I GGNNCC 8 cut(s) 29, 238, 1187, 1420, 2014, 2809, 2810, 4269
PspPPI RGGWCCY 2 cut(s) 2808, 4813
PspXI VCTCGAGB 1 cut(s) 92
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 5 cut(s) 785, 2138, 5915, 6282, 6448
PsuI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5029, 6445
PteI GCGCGC 1 cut(s) 4450
PvuI CGATCG 1 cut(s) 2670
PvuII CAGCTG 5 cut(s) 1616, 4588, 4958, 5912, 6539
RseI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4683, 4689, 4873
SacI GAGCTC 1 cut(s) 4756
SapI GCTCTTC 1 cut(s) 5166
SduI GDGCHC 8 cut(s) 3895, 4366, 4394, 4680, 4756, 6162, 6357, 6614
SfcI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
Sfr274I CTCGAG 3 cut(s) 92, 2492, 4794
SfuI TTCGAA 1 cut(s) 3216
SgrAI CRCCGGYG 1 cut(s) 5319
SinI GGWCC 9 cut(s) 28, 1444, 2723, 2808, 3300, 3665, 4694, 4813, 5788
SlaI CTCGAG 3 cut(s) 92, 2492, 4794
SmiMI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4683, 4689, 4873
SpeI ACTAGT 1 cut(s) 3973
SphI GCATGC 3 cut(s) 722, 4852, 5719
SseBI AGGCCT 1 cut(s) 559
SspI AATATT 3 cut(s) 1947, 3213, 4458
SstI GAGCTC 1 cut(s) 4756
StuI AGGCCT 1 cut(s) 559
StyI CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
TaiI ACGT 9 cut(s) 1165, 2086, 2379, 2845, 3145, 3319, 4629, 4987, 6168
TaqII GACCGA 2 cut(s) 3610, 5954
TatI WGTACW 3 cut(s) 885, 1332, 6412
TauI GCSGC 3 cut(s) 9, 4223, 6104
TseFI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
Tsp45I GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
TspGWI ACGGA 4 cut(s) 840, 3265, 4773, 5285
Van91I CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
Vha464I CTTAAG 2 cut(s) 1196, 4034
VneI GTGCAC 2 cut(s) 4362, 6610
VpaK11BI GGWCC 9 cut(s) 28, 1444, 2723, 2808, 3300, 3665, 4694, 4813, 5788
VspI ATTAAT 4 cut(s) 452, 846, 4671, 5043
XagI CCTNNNNNAGG 1 cut(s) 4169
XceI RCATGY 7 cut(s) 722, 1911, 3165, 4852, 4916, 5719, 6206
XcmI CCANNNNNNNNNTGG 1 cut(s) 5646
XhoI CTCGAG 3 cut(s) 92, 2492, 4794
XmaJI CCTAGG 2 cut(s) 3386, 4595
XmiI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
XmnI GAANNNNTTC 2 cut(s) 2766, 5982
Zsp2I ATGCAT 3 cut(s) 4953, 5721, 6204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.