Rroxscaffold_1G00025840

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001971
Physical Location & Seq
Reverse (-)
32450532 .. 32457746
7215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00025840.1

Sequence Viewer

Length: 1170 bp
ATGATGTCCCATGTGACGTCAGGGTTATGGACTTATGCTGTAGTAATAGAAGTGGATAACAATAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAAAAACAATTCCGCGACGCTTGCCAGGCACTTCAGCTGCCTCCTCGGAATGGAATCATTATCAAGGTAGACTACGTTGGATATGTAAAGGATGCTGAAAAGATTTTGCACCAAGCAATTAATGAGCACAGGTTTGACTACACATTGGTACTTTGGATATCTGATGATGGTACTCCAGATCTAGGAGGCATTAACGAAGAAGGTGGATTTTTTGCAGATGAGACACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAATGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACATGAACTCCGGATCACATACTTCTAATATGCAGGCTGCCTTTGATGAAGCTACATCATCTGCACCTGCATTCCGTGTTTTGAAACAGTTGATCCACCGGTGTCTTGCAGATGCACATACATCTGGAAATGTGTATGCTGATGCACTACTGCAACATCTCCCCCAGTCTAAACTTCATGATCCAGCACTTCAACGCCTACTTCACAAGTATAATTATGGTGGAATTCCGGTTAGAGCTGATGAAAGCATGCATGGTGAATCTCAAGTGGATATTGTATCAAGCTGGAACATTGCTGAATACTTACCCAAGAAAATTCAGGACCATTTTATTTTTATTGTTTCTCAGTTTTTGTATATTCCCTGGAATTATGCACAGAAGCAGTCGGCCGTTAGAGCGACTTTACAGGATGGCAGCAGTTGCACACCATCAATTATGGTTGCAGCTGCTGACACTTTGGAATCTCACATGACCGATTTTCTTAAAGGACAGGAACTGAACAAGTCTGAGAATGACTGTTGGACATCTCCTGGGCTGCCACAACTTGCTGGTAATATCCACAAAGGAGATGCGGCACTAGAGTTCATTAAGAATGTCTGCAGTGTTTTGCTCATTGACCAAAGTGTTGAGCACGACGTTATGGTAATGAGAAGTAATCTGTTAAAATATATGCATGTGAGGGAGTCTGCTCCTGAAGCTCAGTTTTGCGATCCCTGCCCGTCCTTGTTCTTACGAAATGTCATTTGCAGGTACAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

389

Amino Acids

43.44

Weight (kDa)

5.24

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1744 PF08490 110 - 206 6.4e-16 Domain of unknown function (DUF1744)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
AatII GACGTC 1 cut(s) 20
Acc36I ACCTGC 2 cut(s) 484, 1146
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 117
AccIII TCCGGA 1 cut(s) 419
AciI CCGC 2 cut(s) 115, 980
AclWI GGATC 4 cut(s) 430, 496, 584, 1112
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 2 cut(s) 633, 723
AcuI CTGAAG 2 cut(s) 119, 1122
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 3 cut(s) 252, 274, 1160
AfiI CCNNNNNNNGG 2 cut(s) 152, 284
AgeI ACCGGT 1 cut(s) 507
AgsI TTSAA 4 cut(s) 104, 404, 493, 602
AjnI CCWGG 3 cut(s) 126, 770, 937
AluBI AGCT 6 cut(s) 139, 461, 647, 693, 854, 1106
AluI AGCT 6 cut(s) 139, 461, 647, 693, 854, 1106
Alw21I GWGCWC 2 cut(s) 231, 1041
Alw26I GTCTC 1 cut(s) 317
AlwI GGATC 4 cut(s) 430, 496, 584, 1112
AlwNI CAGNNNCTG 2 cut(s) 857, 904
Aor13HI TCCGGA 1 cut(s) 419
AoxI GGCC 1 cut(s) 795
ApeKI GCWGC 6 cut(s) 139, 446, 822, 851, 854, 943
ApoI RAATTY 2 cut(s) 633, 723
AseI ATTAAT 1 cut(s) 222
AsiGI ACCGGT 1 cut(s) 507
AspS9I GGNCC 1 cut(s) 730
AsuHPI GGTGA 2 cut(s) 81, 677
AvaII GGWCC 1 cut(s) 730
BaeI ACNNNNGTAYC 1 cut(s) 1150
BarI GAAGNNNNNNTAC 2 cut(s) 333, 365
Bbv12I GWGCWC 2 cut(s) 231, 1041
BbvI GCAGC 6 cut(s) 126, 433, 834, 841, 863, 930
BccI CCATC 4 cut(s) 100, 263, 812, 844
BceAI ACGGC 1 cut(s) 782
BciT130I CCWGG 3 cut(s) 128, 772, 939
BcoDI GTCTC 1 cut(s) 317
BfaI CTAG 3 cut(s) 284, 395, 986
BfmI CTRYAG 3 cut(s) 39, 339, 1006
BfuAI ACCTGC 2 cut(s) 484, 1146
BglII AGATCT 1 cut(s) 280
BisI GCNGC 7 cut(s) 140, 447, 823, 852, 855, 944, 981
BlsI GCNGC 7 cut(s) 141, 448, 824, 853, 856, 945, 982
Bme1390I CCNGG 3 cut(s) 128, 772, 939
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 730
BmrFI CCNGG 3 cut(s) 128, 772, 939
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 5 cut(s) 184, 334, 511, 541, 967
BmuI ACTGGG 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 146, 770, 938
BsaWI WCCGGW 3 cut(s) 419, 507, 637
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 284
Bse118I RCCGGY 1 cut(s) 507
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 403
BseAI TCCGGA 1 cut(s) 419
BseBI CCWGG 3 cut(s) 128, 772, 939
BseDI CCNNGG 3 cut(s) 146, 770, 938
BseGI GGATG 2 cut(s) 199, 823
BseJI GATNNNNATC 1 cut(s) 403
BseLI CCNNNNNNNGG 2 cut(s) 152, 284
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 3 cut(s) 767, 906, 1121
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 135
BseX3I CGGCCG 1 cut(s) 795
BseXI GCAGC 6 cut(s) 126, 433, 834, 841, 863, 930
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 1 cut(s) 797
BshTI ACCGGT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 798
BsiHKAI GWGCWC 2 cut(s) 231, 1041
BsiSI CCGG 3 cut(s) 420, 508, 638
BslI CCNNNNNNNGG 2 cut(s) 152, 284
BsmAI GTCTC 1 cut(s) 317
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 1 cut(s) 797
Bsp1286I GDGCHC 2 cut(s) 231, 1041
Bsp13I TCCGGA 1 cut(s) 419
Bsp143I GATC 5 cut(s) 280, 422, 501, 589, 1117
BspACI CCGC 2 cut(s) 115, 980
BspANI GGCC 1 cut(s) 797
BspCNI CTCAG 3 cut(s) 766, 907, 1120
BspEI TCCGGA 1 cut(s) 419
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 1 cut(s) 586
BspMAI CTGCAG 1 cut(s) 1010
BspMI ACCTGC 2 cut(s) 484, 1146
BspPI GGATC 4 cut(s) 430, 496, 584, 1112
BspQI GCTCTTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 507
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 3 cut(s) 146, 770, 938
BssMI GATC 5 cut(s) 280, 422, 501, 589, 1117
BssNI GRCGYC 1 cut(s) 17
Bst2UI CCWGG 3 cut(s) 128, 772, 939
Bst4CI ACNGT 3 cut(s) 498, 926, 1166
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 828
BstC8I GCNNGC 3 cut(s) 124, 444, 659
BstDEI CTNAG 3 cut(s) 753, 915, 1107
BstF5I GGATG 2 cut(s) 199, 823
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 5 cut(s) 283, 425, 504, 592, 1120
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 5 cut(s) 280, 422, 501, 589, 1117
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 7 cut(s) 123, 128, 344, 803, 828, 1103, 1122
BstNI CCWGG 3 cut(s) 128, 772, 939
BstNSI RCATGY 2 cut(s) 661, 1085
BstSCI CCNGG 3 cut(s) 126, 770, 937
BstSFI CTRYAG 3 cut(s) 39, 339, 1006
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 6 cut(s) 126, 433, 834, 841, 863, 930
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 795
BsuRI GGCC 1 cut(s) 797
BtsCI GGATG 2 cut(s) 199, 823
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 1 cut(s) 1015
BveI ACCTGC 2 cut(s) 484, 1146
Cac8I GCNNGC 3 cut(s) 124, 444, 659
CaiI CAGNNNCTG 2 cut(s) 857, 904
CciI TCATGA 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 730
CseI GACGC 1 cut(s) 128
Csp6I GTAC 3 cut(s) 251, 273, 1159
CspAI ACCGGT 1 cut(s) 507
CviAII CATG 7 cut(s) 11, 412, 587, 658, 662, 877, 1082
CviQI GTAC 3 cut(s) 251, 273, 1159
DdeI CTNAG 3 cut(s) 753, 915, 1107
DpnI GATC 5 cut(s) 282, 424, 503, 591, 1119
DpnII GATC 5 cut(s) 280, 422, 501, 589, 1117
EaeI YGGCCR 1 cut(s) 795
EagI CGGCCG 1 cut(s) 795
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EclXI CGGCCG 1 cut(s) 795
Eco32I GATATC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 730
Eco52I CGGCCG 1 cut(s) 795
Eco57I CTGAAG 2 cut(s) 119, 1122
EcoRI GAATTC 1 cut(s) 633
EcoRII CCWGG 3 cut(s) 126, 770, 937
EcoRV GATATC 1 cut(s) 261
EcoT22I ATGCAT 2 cut(s) 663, 1083
FaeI CATG 7 cut(s) 14, 415, 590, 661, 665, 880, 1085
FatI CATG 7 cut(s) 10, 411, 586, 657, 661, 876, 1081
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 7 cut(s) 140, 447, 823, 852, 855, 944, 981
FokI GGATG 2 cut(s) 206, 830
Fsp4HI GCNGC 7 cut(s) 140, 447, 823, 852, 855, 944, 981
FspBI CTAG 3 cut(s) 284, 395, 986
GluI GCNGC 7 cut(s) 140, 447, 823, 852, 855, 944, 981
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 1 cut(s) 797
HapII CCGG 3 cut(s) 420, 508, 638
HgaI GACGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 7 cut(s) 14, 415, 590, 661, 665, 880, 1085
HinfI GANTC 5 cut(s) 156, 404, 668, 869, 1091
HpaII CCGG 3 cut(s) 420, 508, 638
HphI GGTGA 2 cut(s) 81, 677
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 5 cut(s) 150, 265, 354, 409, 916
Hpy188III TCNNGA 7 cut(s) 101, 278, 420, 534, 587, 728, 1100
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 2 cut(s) 122, 1046
HpyAV CCTTC 2 cut(s) 296, 374
HpyCH4III ACNGT 3 cut(s) 498, 926, 1166
HpyCH4IV ACGT 3 cut(s) 17, 177, 1044
HpyF10VI GCNNNNNNNGC 7 cut(s) 123, 128, 344, 803, 828, 1103, 1122
HpyF3I CTNAG 3 cut(s) 753, 915, 1107
HpySE526I ACGT 3 cut(s) 17, 177, 1044
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 7 cut(s) 14, 415, 590, 661, 665, 880, 1085
Kpn2I TCCGGA 1 cut(s) 419
Kzo9I GATC 5 cut(s) 280, 422, 501, 589, 1117
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 1102
Lsp1109I GCAGC 6 cut(s) 126, 433, 834, 841, 863, 930
LweI GCATC 5 cut(s) 184, 334, 511, 541, 967
MaeI CTAG 3 cut(s) 284, 395, 986
MaeII ACGT 3 cut(s) 17, 177, 1044
MaeIII GTNAC 2 cut(s) 13, 87
MalI GATC 5 cut(s) 282, 424, 503, 591, 1119
MboI GATC 5 cut(s) 280, 422, 501, 589, 1117
MboII GAAGA 2 cut(s) 311, 341
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 2 cut(s) 231, 1041
MluCI AATT 7 cut(s) 110, 219, 622, 633, 723, 775, 840
MlyI GAGTC 1 cut(s) 1100
MmeI TCCRAC 2 cut(s) 160, 908
MnlI CCTC 5 cut(s) 153, 156, 281, 377, 1080
Mph1103I ATGCAT 2 cut(s) 663, 1083
MroI TCCGGA 1 cut(s) 419
MseI TTAA 6 cut(s) 222, 294, 891, 996, 1070, 1168
MspA1I CMGCKG 2 cut(s) 139, 854
MspI CCGG 3 cut(s) 420, 508, 638
MspR9I CCNGG 3 cut(s) 128, 772, 939
Mva1269I GAATGC 1 cut(s) 479
MvaI CCWGG 3 cut(s) 128, 772, 939
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 7 cut(s) 123, 128, 344, 803, 828, 1103, 1122
NdeII GATC 5 cut(s) 280, 422, 501, 589, 1117
NlaIII CATG 7 cut(s) 14, 415, 590, 661, 665, 880, 1085
NmuCI GTSAC 2 cut(s) 13, 87
NsiI ATGCAT 2 cut(s) 663, 1083
NspI RCATGY 2 cut(s) 661, 1085
PaeI GCATGC 1 cut(s) 661
PagI TCATGA 1 cut(s) 586
PaqCI CACCTGC 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 354
PctI GAATGC 1 cut(s) 479
PfeI GAWTC 4 cut(s) 156, 404, 668, 869
PinAI ACCGGT 1 cut(s) 507
PkrI GCNGC 7 cut(s) 141, 448, 824, 853, 856, 945, 982
PleI GAGTC 1 cut(s) 1099
PpsI GAGTC 1 cut(s) 1099
PshBI ATTAAT 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 126, 770, 937
PspGI CCWGG 3 cut(s) 126, 770, 937
PspPI GGNCC 1 cut(s) 730
PstI CTGCAG 1 cut(s) 1010
PstNI CAGNNNCTG 2 cut(s) 857, 904
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 2 cut(s) 139, 854
RsaI GTAC 3 cut(s) 252, 274, 1160
RsaNI GTAC 3 cut(s) 251, 273, 1159
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 6 cut(s) 222, 294, 891, 996, 1070, 1168
SatI GCNGC 7 cut(s) 140, 447, 823, 852, 855, 944, 981
Sau3AI GATC 5 cut(s) 280, 422, 501, 589, 1117
Sau96I GGNCC 1 cut(s) 730
SchI GAGTC 1 cut(s) 1100
ScrFI CCNGG 3 cut(s) 128, 772, 939
SduI GDGCHC 2 cut(s) 231, 1041
SfaNI GCATC 5 cut(s) 184, 334, 511, 541, 967
SfcI CTRYAG 3 cut(s) 39, 339, 1006
SgrAI CRCCGGYG 1 cut(s) 507
SinI GGWCC 1 cut(s) 730
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
SphI GCATGC 1 cut(s) 661
Sse9I AATT 7 cut(s) 110, 219, 622, 633, 723, 775, 840
SsiI CCGC 2 cut(s) 115, 980
SspMI CTAG 3 cut(s) 284, 395, 986
StyD4I CCNGG 3 cut(s) 126, 770, 937
TaaI ACNGT 3 cut(s) 498, 926, 1166
TaiI ACGT 3 cut(s) 20, 180, 1047
TaqII GACCGA 1 cut(s) 896
TasI AATT 7 cut(s) 110, 219, 622, 633, 723, 775, 840
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 4 cut(s) 156, 404, 668, 869
Tru1I TTAA 6 cut(s) 222, 294, 891, 996, 1070, 1168
Tru9I TTAA 6 cut(s) 222, 294, 891, 996, 1070, 1168
TscAI CASTG 1 cut(s) 1015
TseFI GTSAC 2 cut(s) 13, 87
TseI GCWGC 6 cut(s) 139, 446, 822, 851, 854, 943
Tsp45I GTSAC 2 cut(s) 13, 87
TspDTI ATGAA 6 cut(s) 387, 428, 471, 575, 666, 982
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 1 cut(s) 1015
VpaK11BI GGWCC 1 cut(s) 730
VspI ATTAAT 1 cut(s) 222
XapI RAATTY 2 cut(s) 633, 723
XceI RCATGY 2 cut(s) 661, 1085
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 3 cut(s) 284, 395, 986
ZraI GACGTC 1 cut(s) 18
Zsp2I ATGCAT 2 cut(s) 663, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.