MD03G1139200.v1.1

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001971
Physical Location & Seq
Forward (+)
14419335 .. 14443158
23824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1139200.v1.1.491

Sequence Viewer

Length: 6663 bp
ATGAGCGGCTACCAGAGCCGAAAGCGGGACCGTGGCGACTCATGGGTCCCCAGGAAGCAGAAGGTCATGCGGACCGCCGAGGAGGAGCTGGAGTCCAAGCTAGGGTTCGATCTGTTCTCCGAAGGCGATACCAGGCTCGGCTGGTTGCTCAATTTCGCCACCTCATCTTGGGAGGATCAAGACACCCGTAAAGTATATAGTTGTGTCGATTTGTATTTCGTTTCTCAGGATGGTTCTACTTTTAAGTCAAAATACAAGTTTCGCCCTTATTTCTATGCAGCCACAAAGGAGAAAATGGAAATGGACGTTGAAGCATATTTGAGACGGCGTTATCAAAATCAAATTGCTGATGTTCAAATAGTGCAGAAAGAAGATCTTGATCTCAAAAATCATTTGTCTGGCCTTCGCAAGTCCTACCTAAAGCTGTCGTTTGATAATGTTCAACAACTAATGAACGTCAAGAGTGACTTGCTGCATGTCGTCGAAAGAAACCGCACCAAATTTGATGCTGCAGAAGCTTATGAGTCAATATTAATTGGGAAACGAGAACAAAGGACTCAAGATTTTTTAGATTGTATTGTTGATCTTCGTGAATATGATGTTCCTTACCATGTCCGCTTTGCCATTGACAATGATGTACGATGTGGGCAGTGGTATGATGTTAGTGTATCCAGTAGTGGCTCTGTGCTTAAGATGAGGACAGATCTTCTCCAGCGTGCTGAAGTTCATGTTTGTGCATTTGATATTGAGACGACGAAGCTACCTTTGAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGACGGACAAGGTTACTTGATTATTAACAGAGAGTGTGTTGGGGATGATATAGAGGATTTGGAGTACACTCCAAAACCAGAATTTGAAGGGTTTTTCAAGGTGACAAATGTGAAGAATGAGGTAGAACTTCTAGGAAAATGGTTTTGCCATATGCGGGAGGTGAAGCCTGGAATTTATGTCACATACAATGGTGATTTTTTCGACTGGCCTTTCATGGAAACCAGAGCCGCGTATCATGGGTTTAAACTGAGTGATGAGGTTGGATTTCAATGTGACAAGAATCAGGGGGAGTGCCGAGCTAAATTTGCTTGCCATCTTGACTGTTTTGCATGGGTCAAACGTGATAGTTATCTTCCTCAGGGGAGCCAGGGCCTTAAGGCTGTTACAAAGGCAAAGTTAGGTTATCATCCACTGGAGGTGAATCCTGAGGATATGGTTCGCTTTGCAAAGGAGAAACCTCAGATGATGGCATCCTATTCTGTTTCTGATGCCGTTGCGACTTATTACTTGTATATGACTTATGTGCATCCATTCATTTTCTCTCTCGCAACTATTATTCCAATGTCTCCGGACGAGGTTTTGCGCAAGGGTAGTGGAACTCTTTGTGAAATGCTTCTCATGGTCCAGGCATACAAGGCAAATGTTATCTGCCCTAACAAACATCAATCTGATCCCGAAAAGTTCTATAAAAATCACCTTTTAGAGAGTGAGACTTATATAGGTGGCCATGTTGAATGCCTTGAAAGTGGTGTTTTTAGATCTGATATTCCAACTAGCTTTAAGCTTGATGGGTCTGCCTATGAGCAATTGATCAACAATCTTGATCGAGATCTTCAATATGCTATAAGAGTAGAGGGTAAGATGGACATTGATTCAGTATCCAATTATGAAGAAGTAAAGAGATCTATATTGGAAAAGCTTGTGGGGTTGCGAGATGAACCTATACGTGAAGAGTGCCCCCTTATTTATCATCTGGATGTTGCTGCAATGTATCCCAACATCATTTTGACAAACAGACTTCAGCCATCATCAATAGTTACAGATGAGATTTGCACTGCTTGTGATTTCAATCGCCCAGGTAAAACATGTCTTCGCAACCTTGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAAGAAAAGTGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTTTGTTGATGGTGGCAATTCCAAGCTGTCAAAATCTTTTCTTGAGTTGCCTAAAATGGAACAACAATCAAGGCTCAAAGAGCGCCTTAAAAAATACTGTCAGAAGGCGTATAGACGAGTAGTGGACAAGCCAGTCAGTGAAGTTCGGGAAGCAGGGATCTGCATGAGGGAAAATTCATTTTATGTTGATACAGTTCGAAGCTTTCGAGATAGAAGGTATGAGTACAAGGGGCTTAATAAGATTTGGAAGGGGAAGTTGTCTGAAGCCAAGGCTAGTGGAAATTCTATAAAAATCCAGGAAGCACAAGATATGGTAGTGCTTTACGATTCATTACAACTTGCACATAAGTGTATACTTAATTCCTTTTATGGATATGTCATGCGCAAGGGTGCGAGATGGTATTCTATGGAAATGGCTGGAGTAGTTACATATACTGGAGCCAAAATTATTCAAAACGCTCGCTTGCTGGTTGAGAAAATTGGAAAACCTCTTGAGTTAGACACAGATGGTATCTGGTGTGCGCTTCCTGGATCTTTTCCAGAGAACTTTACATTCAAAACAAAAGACACGAAGAAGAAGCTGACTATCTCATATCCCTGTGTAATGCTTAACGTTGATGTGGCAAGAAACAATACAAATGACCAATACCAGACTCTTACAGATCCTTTTAAGAATACTTACACAACTCATAGTGAGTGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTCTTCCTGCTTCTAAAGAAGAAGGCATTCTAATTAAGAAGCGATATGCTGTTTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTTGAGATCAAGCGTAGGGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAGCTTTTTGATAAGTTCCTTCACGGTTCTACTTTAGAGGAATGCTATTCCGCAGTTGCTTCTGTCGCCAACCGTTGGCTGGATCTCCTTGATAATCAAGGAAAGGATATTGCAGACGGTGAATTGCTGGATTATATATCAGAGTCAAGCACCATGAGCAAGTCTTTAGTAGACTATGGAGAGCAAAAGTCATGTGCAGTGACCACTGCAAGACGTCTTGCTGATTTTCTTGGTGATACGATGGTCAAAGAGAAAGGACTGCGTTGCCAGTATATAGTTGCATGTGAACCAAAGGGCACACCTGTTAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTATGTACGGAAGTGGTGCAAGGTTTCATCAGATGTTGGCATTCGATCCATTATTGACTGGTCCTATTACAAACAACGCCTTGGTTCGGCAATTCAGAAAATTGTCACTATTCCAGCTGCAATGCAAAAGGTTGCAAATCCAGTTCCTAGAGTAGCTCATCCTGATTGGCTGCATAAGAAGGTTCGTGAGAAGGAGGACAAATTTCGCCAGCGCAAGTTGATTGATATCTTCAAGTCATTGAACAGGGATCATATGCTAGAAAAGAGCAGTGATGCTGTTGACTCTAGTCATGTGCTTGGTCAAGAGATTGTTGATGATTTGGAAGACTTCAACAAAAAAAACATTTCCATAAATGGACCCAGACCAATTGTTCGCTGTTATGAAGTCAATCGCAAGAAAAATTCAGTTGAAACAACTTGCCAAATAGATTCACAGCAACAACAAACTAATAATAAAGAAAATGAACATCAAGAGCAAAATGGTGTCCCTTTACAGTGCATTGATAGAAATATGGATTATCATGGTTGGCTTGAAACGAAGAAGAGAAAATGGAAAGATACTCTTGACAGGAGGAAAAAGCAAAGATTGAATGACGCAAGGTCTGCTCACCATGCTAATATTGAGCTGCTTGGTGGTGGTAGAAATAAGAAAGGAACTCAGGGAAAAATAGGTGTGAATTCATATTTTAGGAGGCATGAAGAAGCCCTAACACGCTGTCATTGGCAGATATTACAACTAGTGCCCAGTTCTCAGGGTGACCAATTTTACGCTTGGGTGGTTATTGAAGGAATCATGCTTAAGATCCCTGTTACTGTTCCAAGAGTTTTTTACATTAATTCTAAGGCACCTATTGAAAAAATATTTAATAAAATATCTGAAGAAATACCTCCAGTAAAGGATGTCAAAAAGGCTCTTCCTCATGGAAGGCGTATCTACAACCTATATGAGGCTACAATTGATGAGGGTCAGTTTAGGACAATTAGAAAAAGGCTTGCAGCTCTTCTTGGCGATCCAGAAGTTGAGGGAATATATGAAACAAAGGTACCCTTGGAGTTCAATGCAATCATTCAGATTGGTTGTGTTTGCAAAGTGGATAAAACTGCTAAGAAAAGAAACGTCCAAGATGGTTGGAGTGTGAGTGAACTGCACATGAAGACAACTACAGAGTGCTCATATCTGGACCAATCCATCTCTTTCTTTTACTTGTATCAGAGCACATCTGAAGGGCGAGCTATATATATTGCCTATTTTCCTGCATCAACAACAATAAAGGTTGTGGTGGTAAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTGGAAAAATTATTTCGTGATGCTTGCCAAACATTATCAGTTCAGCCACCTCCTAGGAATGGTATCATTATAAAGGTGGACTATGTTGGATTCGTCAAGGATGCTGAAACAGTTTTGCACCGTTCGATTAATGAGTACAGACATGAATATCCTGGACCTACAATTGCTGTTATCGAATGCCCAAATTTTCAAATGATGAAGTCAGGTATAGGAGCTCTTGATGATTTTCCGTGTGTGAGCATTCCCTCTAATGCTCGTGATAGTCTCTATCAGGTTCTTGGGTGGCAGCAAGTGGCTGCAAAAATTGGAATGCAGCGTTGTGCTGCATCACCTCAGTGGTTGAATGAGAGAATTTCACTCTCAAGATATGCCCATGTTCCATTGGGAAATTTCGAACTTGACTGGCTTATACATACAGCAGATATCTTCTTTTCAAGAGCACTACGTGATCAACAACAGGTGCTTTGGATATCTGATGATGGCATTCCAGATCTAGGAGGCATTAATGAAGAAGATTCATGTTTTGCTGATGAGGTCTGTGAACTTAATTTTTCTTACCCTGGAGCATATAGGAAGATCACTGTGGAGCTAAAGATACATCATCTAGCTGTAAATGCTCTTTTAAAAAGCAACCAGGTGAATGAAATGGAAGGAGGTTCTTTGCTAGGATTTGAATCAGATGCGAACTCCGGAGTACATACTTTTGATGCACAAGTTGGCTTTGATGAAGCTACCTCATGTGCTCCTACATTCCGTGTCCTGAAACAGTTGATTCAGAGGTGTCTTTCAAATGCAGTTACATCTGGAAATGTGTACGCTGATGCACTATTGCAACATTTATATCGATGGCTTTGCAGCCCCCAGTCTAAGCTTCATGATCCATCTCTTCACCGCATACTTCATAAGGTCATGAAAAAGGTGCTCGCAATTTTAATGACTGAGTTCCGCAAATTGGGTGCCAAGATCATATTCGCGAATTTCTACAAGGTTATTATAGACACAGGAAAACTTGACCTATCAGCTGCCAAAGCGTATTGTGATAGCTTGCTTAAAACTCTGCAAACAAGAGAACTATTTGAGTGGATAGAGCTTGAACCACTACACTTCTGGCATTCTTTGCTTTTCATGGATCAGTTTAACTATGGTGGAATTCCGGTTGGAGCTGATGGAAGCATGCATGGTGAAGCTCAAATGGATATTGTATCAAGCTGGAATATCGCTGAATACTTGCCCAAGAAAATTCAGGACCACTTTGTTTATATTGTCTCTCAGTATCTGTATATTCCCTGGGATTATGCACAGAAGCAGGCAGCAGTTAGAACATCTTTACAGAGTGACAGCATTTGCACGCCATCAATCAATGTAGCAGATGCTGAGACTTTTGAGTCTCATATGATAGATTTCCTTAAAGGACAGATCGGCTCATATTTTACAGATAAACTTCTGGGAATTGTTCATGATACCATGCTTCATATGAAGGAACTGAAAAAATCAGAGAATGACCATTGTACATCTCTTGAGCTGCCACAAATTGCTGGTAATATCCACCGAGGGGATGCAGCACTGGAGTTCATCAAGCACGTCTGCGGTGTTTTCTCAATTGACCAAAATGTTGTCCATGATGTTTTGGTAATGAGAAAGAATCTGTTGAAGATTGTACGTGTGAGGGAGTTTGCCCCCGAAGCAGAGTTTCATGATCCCGGCCCAACCTTCACTTTACCAAATGTCATTTGCAGCTACTGCAACGATTGTAGAGACCTGGACTTGTGCCGTGACTCAGCTCTTCTAGCTGGGGAGTGGCGTTGCTGTGTACCACAATGCGGGCAGCTTTATGACAAAGAGGTAATGGAGAACGCCCTTCTTCAGATTGTGCGGCAGAGAGAGCGTCTTTACCACCTACAAGATCTCGAATGCCTCAGGTGTCACCAAGTGAAAGCTGCACGCTTAGCAGAGCAATGTGTATGCGCTGGCTCATATAAGTGCAGGCAAAACGCAACTGAATTTCGTACCCAGATGGAGTTAATTTGGAAGATTGCCATTCATCAGAAATTTCAACTTCTCCAAGAGTGCACTGAATGGATTTTAGAAGTGAGCAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2221

Amino Acids

254.96

Weight (kDa)

6.62

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 195 - 401 8.9e-56 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 752 - 1072 3.8e-18 DNA polymerase family B
POL2_thumb PF22634 947 - 1122 1.1e-76 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1540 - 1904 1.2e-114 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2081 - 2147 5.4e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2153 - 2209 1e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4631
AasI GACNNNNNNGTC 3 cut(s) 44, 2129, 5944
AatII GACGTC 1 cut(s) 3094
Acc16I TGCGCA 2 cut(s) 1403, 2381
Acc65I GGTACC 1 cut(s) 4282
AccB1I GGYRCC 3 cut(s) 4084, 4282, 5516
AccB7I CCANNNNNTGG 1 cut(s) 2952
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 2350, 3048
AccII CGCG 2 cut(s) 1049, 5534
AccIII TCCGGA 2 cut(s) 1387, 5249
AclI AACGTT 1 cut(s) 2610
AcoI YGGCCR 1 cut(s) 1543
AcuI CTGAAG 7 cut(s) 741, 1823, 2280, 2873, 4137, 4482, 6407
AcyI GRCGYC 1 cut(s) 3091
AdeI CACNNNGTG 2 cut(s) 5006, 6490
AflII CTTAAG 3 cut(s) 689, 1193, 4037
AflIII ACRYGT 2 cut(s) 1904, 6220
AhdI GACNNNNNGTC 1 cut(s) 3838
AhlI ACTAGT 1 cut(s) 3976
AjiI CACGTC 1 cut(s) 6142
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 5513, 5545
AleI CACNNNNGTG 2 cut(s) 2344, 4894
Alw21I GWGCWC 7 cut(s) 4412, 4457, 4777, 5002, 5305, 5484, 6632
Alw26I GTCTC 9 cut(s) 316, 743, 1389, 1523, 4829, 5830, 5930, 5952, 6309
Alw44I GTGCAC 1 cut(s) 6628
AlwNI CAGNNNCTG 5 cut(s) 782, 3224, 5836, 5933, 6300
Aor13HI TCCGGA 2 cut(s) 1387, 5249
AoxI GGCC 5 cut(s) 400, 1025, 1189, 1543, 6262
ApaLI GTGCAC 1 cut(s) 6628
ArsI GACNNNNNNTTYG 2 cut(s) 3594, 3626
AseI ATTAAT 4 cut(s) 533, 4074, 4689, 5064
Asp700I GAANNNNTTC 2 cut(s) 1431, 2763
Asp718I GGTACC 1 cut(s) 4282
AspA2I CCTAGG 1 cut(s) 4613
AspLEI GCGC 6 cut(s) 1404, 2082, 2382, 2521, 3453, 6527
AsuC2I CCSGG 1 cut(s) 6261
AsuII TTCGAA 2 cut(s) 2194, 4953
AvrII CCTAGG 1 cut(s) 4613
AxyI CCTNAGG 3 cut(s) 1176, 1245, 6476
BaeGI GKGCMC 4 cut(s) 1778, 3176, 3984, 6632
BaeI ACNNNNGTAYC 4 cut(s) 3105, 3138, 4266, 4299
BalI TGGCCA 1 cut(s) 1545
BanI GGYRCC 3 cut(s) 4084, 4282, 5516
BanII GRGCYC 1 cut(s) 4777
BauI CACGAG 1 cut(s) 4815
BbsI GAAGAC 3 cut(s) 1901, 3570, 4400
Bbv12I GWGCWC 7 cut(s) 4412, 4457, 4777, 5002, 5305, 5484, 6632
BceAI ACGGC 3 cut(s) 341, 1295, 6315
BcgI CGANNNNNNTGC 2 cut(s) 5394, 5428
BciVI GTATCC 3 cut(s) 679, 1708, 1821
BclI TGATCA 2 cut(s) 1629, 5008
BcnI CCSGG 1 cut(s) 6261
BcoDI GTCTC 9 cut(s) 316, 743, 1389, 1523, 4829, 5830, 5930, 5952, 6309
BcuI ACTAGT 1 cut(s) 3976
BfmI CTRYAG 2 cut(s) 510, 4401
BfoI RGCGCY 1 cut(s) 2083
BfrI CTTAAG 3 cut(s) 689, 1193, 4037
BfuI GTATCC 3 cut(s) 679, 1708, 1821
BglII AGATCT 7 cut(s) 373, 703, 1577, 1648, 1721, 5050, 6463
BlnI CCTAGG 1 cut(s) 4613
BlpI GCTNAGC 2 cut(s) 2877, 6505
BmeRI GACNNNNNGTC 1 cut(s) 3838
BmgBI CACGTC 1 cut(s) 6142
BmrI ACTGGG 2 cut(s) 3978, 5416
BmuI ACTGGG 2 cut(s) 3978, 5416
BoxI GACNNNNGTC 1 cut(s) 3525
BpiI GAAGAC 3 cut(s) 1901, 3570, 4400
BpmI CTGGAG 8 cut(s) 110, 695, 1253, 2436, 2454, 4113, 5142, 6146
Bpu1102I GCTNAGC 2 cut(s) 2877, 6505
Bpu14I TTCGAA 2 cut(s) 2194, 4953
BpuEI CTTGAG 5 cut(s) 543, 2060, 2510, 4906, 6098
BpuMI CCSGG 1 cut(s) 6261
Bsa29I ATCGAT 1 cut(s) 5404
BsaAI YACGTR 3 cut(s) 1766, 5006, 6221
BsaBI GATNNNNATC 6 cut(s) 378, 1167, 1647, 1887, 3464, 5233
BsaHI GRCGYC 1 cut(s) 3091
BsaI GGTCTC 1 cut(s) 6309
BsaWI WCCGGW 3 cut(s) 1387, 5249, 5713
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 4363, 4393
Bse21I CCTNAGG 3 cut(s) 1176, 1245, 6476
Bse3DI GCAATG 4 cut(s) 1812, 2739, 3366, 6521
Bse8I GATNNNNATC 6 cut(s) 378, 1167, 1647, 1887, 3464, 5233
BseAI TCCGGA 2 cut(s) 1387, 5249
BseCI ATCGAT 1 cut(s) 5404
BseJI GATNNNNATC 6 cut(s) 378, 1167, 1647, 1887, 3464, 5233
BseMI GCAATG 4 cut(s) 1812, 2739, 3366, 6521
BseRI GAGGAG 2 cut(s) 95, 98
BseSI GKGCMC 4 cut(s) 1778, 3176, 3984, 6632
BseYI CCCAGC 1 cut(s) 6350
BsgI GTGCAG 5 cut(s) 383, 3093, 4370, 6483, 6562
Bsh1236I CGCG 2 cut(s) 1049, 5534
BshFI GGCC 5 cut(s) 402, 1027, 1191, 1545, 6264
BshNI GGYRCC 3 cut(s) 4084, 4282, 5516
BshVI ATCGAT 1 cut(s) 5404
BsiHKAI GWGCWC 7 cut(s) 4412, 4457, 4777, 5002, 5305, 5484, 6632
BsiSI CCGG 4 cut(s) 1388, 5250, 5714, 6261
BslFI GGGAC 4 cut(s) 32, 41, 2818, 3710
BsmAI GTCTC 9 cut(s) 316, 743, 1389, 1523, 4829, 5830, 5930, 5952, 6309
BsmBI CGTCTC 2 cut(s) 316, 743
BsmFI GGGAC 4 cut(s) 32, 41, 2818, 3710
BsnI GGCC 5 cut(s) 402, 1027, 1191, 1545, 6264
Bso31I GGTCTC 1 cut(s) 6309
Bsp119I TTCGAA 2 cut(s) 2194, 4953
Bsp13I TCCGGA 2 cut(s) 1387, 5249
Bsp1407I TGTACA 1 cut(s) 6068
Bsp1720I GCTNAGC 2 cut(s) 2877, 6505
Bsp68I TCGCGA 1 cut(s) 5534
BspANI GGCC 5 cut(s) 402, 1027, 1191, 1545, 6264
BspDI ATCGAT 1 cut(s) 5404
BspEI TCCGGA 2 cut(s) 1387, 5249
BspFNI CGCG 2 cut(s) 1049, 5534
BspHI TCATGA 4 cut(s) 5434, 5469, 6016, 6253
BspMAI CTGCAG 1 cut(s) 514
BspQI GCTCTTC 3 cut(s) 4158, 4245, 6348
BspT104I TTCGAA 2 cut(s) 2194, 4953
BspT107I GGYRCC 3 cut(s) 4084, 4282, 5516
BspTI CTTAAG 3 cut(s) 689, 1193, 4037
BspTNI GGTCTC 1 cut(s) 6309
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 1812, 2739, 3366, 6521
BsrGI TGTACA 1 cut(s) 6068
BssNAI GTATAC 1 cut(s) 2351
BssNI GRCGYC 1 cut(s) 3091
BssSI CACGAG 1 cut(s) 4815
BssT1I CCWWGG 4 cut(s) 2265, 3319, 4287, 4613
Bst1107I GTATAC 1 cut(s) 2351
Bst2BI CACGAG 1 cut(s) 4815
Bst6I CTCTTC 6 cut(s) 1764, 3776, 4158, 4245, 5451, 6348
BstACI GRCGYC 1 cut(s) 3091
BstAFI CTTAAG 3 cut(s) 689, 1193, 4037
BstAPI GCANNNNNTGC 3 cut(s) 3842, 5677, 6300
BstAUI TGTACA 1 cut(s) 6068
BstBAI YACGTR 3 cut(s) 1766, 5006, 6221
BstBI TTCGAA 2 cut(s) 2194, 4953
BstDSI CCRYGG 1 cut(s) 31
BstEII GGTNACC 1 cut(s) 3995
BstENI CCTNNNNNAGG 1 cut(s) 4184
BstFNI CGCG 2 cut(s) 1049, 5534
BstH2I RGCGCY 1 cut(s) 2083
BstHHI GCGC 6 cut(s) 1404, 2082, 2382, 2521, 3453, 6527
BstMAI GTCTC 9 cut(s) 316, 743, 1389, 1523, 4829, 5830, 5930, 5952, 6309
BstNSI RCATGY 4 cut(s) 479, 1908, 3162, 5737
BstPAI GACNNNNGTC 1 cut(s) 3525
BstPI GGTNACC 1 cut(s) 3995
BstSFI CTRYAG 2 cut(s) 510, 4401
BstSLI GKGCMC 4 cut(s) 1778, 3176, 3984, 6632
BstUI CGCG 2 cut(s) 1049, 5534
BstV2I GAAGAC 3 cut(s) 1901, 3570, 4400
BstZ17I GTATAC 1 cut(s) 2351
Bsu15I ATCGAT 1 cut(s) 5404
Bsu36I CCTNAGG 3 cut(s) 1176, 1245, 6476
BsuI GTATCC 3 cut(s) 679, 1708, 1821
BsuRI GGCC 5 cut(s) 402, 1027, 1191, 1545, 6264
BsuTUI ATCGAT 1 cut(s) 5404
BtgI CCRYGG 1 cut(s) 31
BtrI CACGTC 1 cut(s) 6142
BtsI GCAGTG 5 cut(s) 656, 1872, 3081, 3081, 3514
BtuMI TCGCGA 1 cut(s) 5534
CaiI CAGNNNCTG 5 cut(s) 782, 3224, 5836, 5933, 6300
CciI TCATGA 4 cut(s) 5434, 5469, 6016, 6253
CfoI GCGC 6 cut(s) 1404, 2082, 2382, 2521, 3453, 6527
ClaI ATCGAT 1 cut(s) 5404
CpoI CGGWCCG 1 cut(s) 72
CseI GACGC 2 cut(s) 3842, 6434
CsiI ACCWGGT 1 cut(s) 5193
CspCI CAANNNNNGTGG 4 cut(s) 1393, 1428, 2254, 2289
CspI CGGWCCG 1 cut(s) 72
DraI TTTAAA 2 cut(s) 1063, 5184
DraIII CACNNNGTG 2 cut(s) 5006, 6490
DrdI GACNNNNNNGTC 3 cut(s) 44, 2129, 5944
DriI GACNNNNNGTC 1 cut(s) 3838
DseDI GACNNNNNNGTC 3 cut(s) 44, 2129, 5944
EaeI YGGCCR 1 cut(s) 1543
Eam1104I CTCTTC 6 cut(s) 1764, 3776, 4158, 4245, 5451, 6348
Eam1105I GACNNNNNGTC 1 cut(s) 3838
EarI CTCTTC 6 cut(s) 1764, 3776, 4158, 4245, 5451, 6348
Ecl136II GAGCTC 1 cut(s) 4775
Eco130I CCWWGG 4 cut(s) 2265, 3319, 4287, 4613
Eco24I GRGCYC 1 cut(s) 4777
Eco31I GGTCTC 1 cut(s) 6309
Eco32I GATATC 3 cut(s) 3466, 4984, 5031
Eco53kI GAGCTC 1 cut(s) 4775
Eco57I CTGAAG 7 cut(s) 741, 1823, 2280, 2873, 4137, 4482, 6407
Eco81I CCTNAGG 3 cut(s) 1176, 1245, 6476
Eco91I GGTNACC 1 cut(s) 3995
EcoICRI GAGCTC 1 cut(s) 4775
EcoNI CCTNNNNNAGG 1 cut(s) 4184
EcoO109I RGGNCCY 3 cut(s) 46, 1189, 2805
EcoO65I GGTNACC 1 cut(s) 3995
EcoRI GAATTC 2 cut(s) 3916, 5709
EcoRV GATATC 3 cut(s) 3466, 4984, 5031
EcoT14I CCWWGG 4 cut(s) 2265, 3319, 4287, 4613
EcoT22I ATGCAT 1 cut(s) 5739
EcoT38I GRGCYC 1 cut(s) 4777
ErhI CCWWGG 4 cut(s) 2265, 3319, 4287, 4613
Esp3I CGTCTC 2 cut(s) 316, 743
FaqI GGGAC 4 cut(s) 32, 41, 2818, 3710
FauI CCCGC 3 cut(s) 18, 966, 6374
FauNDI CATATG 4 cut(s) 969, 3492, 5952, 6033
FbaI TGATCA 2 cut(s) 1629, 5008
FblI GTMKAC 2 cut(s) 2350, 3048
FriOI GRGCYC 1 cut(s) 4777
FspI TGCGCA 2 cut(s) 1403, 2381
GlaI GCGC 6 cut(s) 1403, 2081, 2381, 2520, 3452, 6526
GsaI CCCAGC 1 cut(s) 6354
GsuI CTGGAG 8 cut(s) 110, 695, 1253, 2436, 2454, 4113, 5142, 6146
HaeII RGCGCY 1 cut(s) 2083
HaeIII GGCC 5 cut(s) 402, 1027, 1191, 1545, 6264
HapII CCGG 4 cut(s) 1388, 5250, 5714, 6261
HgaI GACGC 2 cut(s) 3842, 6434
HhaI GCGC 6 cut(s) 1404, 2082, 2382, 2521, 3453, 6527
Hin1I GRCGYC 1 cut(s) 3091
Hin6I GCGC 6 cut(s) 1402, 2080, 2380, 2519, 3451, 6525
HinP1I GCGC 6 cut(s) 1402, 2080, 2380, 2519, 3451, 6525
HincII GTYRAC 2 cut(s) 820, 3520
HindII GTYRAC 2 cut(s) 820, 3520
HindIII AAGCTT 5 cut(s) 516, 1601, 1736, 2197, 5429
HpaII CCGG 4 cut(s) 1388, 5250, 5714, 6261
Hpy99I CGWCG 2 cut(s) 485, 757
Hsp92I GRCGYC 1 cut(s) 3091
HspAI GCGC 6 cut(s) 1402, 2080, 2380, 2519, 3451, 6525
KflI GGGWCCC 2 cut(s) 46, 2805
Kpn2I TCCGGA 2 cut(s) 1387, 5249
KpnI GGTACC 1 cut(s) 4286
Ksp22I TGATCA 2 cut(s) 1629, 5008
LguI GCTCTTC 3 cut(s) 4158, 4245, 6348
LmnI GCTCC 8 cut(s) 85, 1182, 2435, 4772, 5123, 5146, 5308, 5720
MabI ACCWGGT 1 cut(s) 5193
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 6 cut(s) 1625, 2700, 3606, 4194, 4722, 6159
MlsI TGGCCA 1 cut(s) 1545
MluNI TGGCCA 1 cut(s) 1545
MmeI TCCRAC 5 cut(s) 1060, 1613, 4349, 4627, 5698
Mox20I TGGCCA 1 cut(s) 1545
Mph1103I ATGCAT 1 cut(s) 5739
MroI TCCGGA 2 cut(s) 1387, 5249
MroXI GAANNNNTTC 2 cut(s) 1431, 2763
MscI TGGCCA 1 cut(s) 1545
MslI CAYNNNNRTG 5 cut(s) 732, 1794, 2344, 4894, 6538
Msp20I TGGCCA 1 cut(s) 1545
MspA1I CMGCKG 2 cut(s) 3356, 5582
MspCI CTTAAG 3 cut(s) 689, 1193, 4037
MspI CCGG 4 cut(s) 1388, 5250, 5714, 6261
MssI GTTTAAAC 1 cut(s) 1063
MunI CAATTG 6 cut(s) 1625, 2700, 3606, 4194, 4722, 6159
MvnI CGCG 2 cut(s) 1049, 5534
NciI CCSGG 1 cut(s) 6261
NdeI CATATG 4 cut(s) 969, 3492, 5952, 6033
NmeAIII GCCGAG 3 cut(s) 103, 117, 1139
NruI TCGCGA 1 cut(s) 5534
NsbI TGCGCA 2 cut(s) 1403, 2381
NsiI ATGCAT 1 cut(s) 5739
NspI RCATGY 4 cut(s) 479, 1908, 3162, 5737
NspV TTCGAA 2 cut(s) 2194, 4953
OliI CACNNNNGTG 2 cut(s) 2344, 4894
PaeI GCATGC 1 cut(s) 5737
PagI TCATGA 4 cut(s) 5434, 5469, 6016, 6253
PasI CCCWGGG 1 cut(s) 5847
PciI ACATGT 1 cut(s) 1904
PciSI GCTCTTC 3 cut(s) 4158, 4245, 6348
PcsI WCGNNNNNNNCGW 1 cut(s) 2200
PdmI GAANNNNTTC 2 cut(s) 1431, 2763
PflMI CCANNNNNTGG 1 cut(s) 2952
PfoI TCCNGGA 3 cut(s) 2292, 2524, 4711
PmeI GTTTAAAC 1 cut(s) 1063
Ppu21I YACGTR 3 cut(s) 1766, 5006, 6221
PpuMI RGGWCCY 2 cut(s) 46, 2805
PscI ACATGT 1 cut(s) 1904
PshAI GACNNNNGTC 1 cut(s) 3525
PshBI ATTAAT 4 cut(s) 533, 4074, 4689, 5064
PsiI TTATAA 1 cut(s) 4631
Psp124BI GAGCTC 1 cut(s) 4777
Psp1406I AACGTT 1 cut(s) 2610
Psp5II RGGWCCY 2 cut(s) 46, 2805
PspEI GGTNACC 1 cut(s) 3995
PspFI CCCAGC 1 cut(s) 6350
PspPPI RGGWCCY 2 cut(s) 46, 2805
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 5 cut(s) 782, 3224, 5836, 5933, 6300
PvuII CAGCTG 2 cut(s) 3356, 5582
RruI TCGCGA 1 cut(s) 5534
RseI CAYNNNNRTG 5 cut(s) 732, 1794, 2344, 4894, 6538
Rsr2I CGGWCCG 1 cut(s) 72
RsrII CGGWCCG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 4777
SapI GCTCTTC 3 cut(s) 4158, 4245, 6348
SexAI ACCWGGT 1 cut(s) 5193
SfcI CTRYAG 2 cut(s) 510, 4401
SfuI TTCGAA 2 cut(s) 2194, 4953
SmiMI CAYNNNNRTG 5 cut(s) 732, 1794, 2344, 4894, 6538
SmlI CTYRAG 8 cut(s) 558, 689, 1193, 2039, 2489, 4037, 4921, 6077
SmoI CTYRAG 8 cut(s) 558, 689, 1193, 2039, 2489, 4037, 4921, 6077
SpeI ACTAGT 1 cut(s) 3976
SphI GCATGC 1 cut(s) 5737
SspI AATATT 5 cut(s) 531, 1944, 3210, 3859, 4101
SstI GAGCTC 1 cut(s) 4777
StyI CCWWGG 4 cut(s) 2265, 3319, 4287, 4613
TatI WGTACW 5 cut(s) 882, 2220, 4695, 5254, 6068
TauI GCSGC 3 cut(s) 9, 1049, 6436
TspGWI ACGGA 4 cut(s) 837, 3262, 4779, 5303
Van91I CCANNNNNTGG 1 cut(s) 2952
Vha464I CTTAAG 3 cut(s) 689, 1193, 4037
VneI GTGCAC 1 cut(s) 6628
VspI ATTAAT 4 cut(s) 533, 4074, 4689, 5064
XagI CCTNNNNNAGG 1 cut(s) 4184
XceI RCATGY 4 cut(s) 479, 1908, 3162, 5737
XcmI CCANNNNNNNNNTGG 2 cut(s) 2953, 5664
XmaJI CCTAGG 1 cut(s) 4613
XmiI GTMKAC 2 cut(s) 2350, 3048
XmnI GAANNNNTTC 2 cut(s) 1431, 2763
ZraI GACGTC 1 cut(s) 3092
Zsp2I ATGCAT 1 cut(s) 5739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.