Rroxscaffold_1G00026110

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001971
Physical Location & Seq
Reverse (-)
32837932 .. 32840508
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026110.1

Sequence Viewer

Length: 900 bp
ATGGGGATGATCAGCAAGAGGCGGAGATCACGAAAATGGGAAGGATCGTCGAGGGGACGCCGTAAATGGGTTTTCCGGCCGGCTCGGGTCCATCAACCATCTGTAATTACTAAACCTGGAGCATATAGAAAGGTCACAGTGGAGCTAAAAATACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAATGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACATGAACTCTGGATCACATACTTCTAATATGCAGGCTGCCTTTGATGAAGCTACATCATCTGCACCTGCATTCCGTGTTTTGAAACAGTTGATCCACCGGTGTCTTGCAGATGCACATACATCTGGAAATGTGTATGCTGATGCACTACTGCAACATCTGTATCGATGGCTTTGCAGCCCTGAGTCTAAACTTCATGATCCAGCACTTCAACGCCTACTTCACAAGGTCATGAAAAAGGTGCTTTTGATTTTATTGGATGAGTTCTGCAAATTGGGTGCAAATATCATATTTGCAAATTTCTATAAGGTTATTATAGACACCGGAAAATTTGATATATCCGCAGCCAAAGCTTATTGTGATAGTGTGCTTAAAACTCTCCAGAAAAGAGCTGATGAAAGCATGCATGGTGAATCTCAAGTGGATATTGTATCGAGCTGGAACATTGCTGAATACTTACCCAAGAAAATTCAGGACCATTTTATTTTTATTGTTTCTCAGTTTTTGTATATTCCATGGAATTATGCACAGAAGCAGTCGGCCGTTAGAGCGACTTTACAGGATGGCAGCAGTTGCACGCCATCAATTACGGTTGCAGCTGCTGAGACTTTGGAATCTCACATGACCGATTTTCTTAAAGGACAGTTTTGTATGGATGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

299

Amino Acids

33.71

Weight (kDa)

9.22

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1744 PF08490 32 - 208 7.6e-54 Domain of unknown function (DUF1744)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
AciI CCGC 2 cut(s) 22, 576
AclWI GGATC 4 cut(s) 52, 256, 322, 428
AcoI YGGCCR 2 cut(s) 77, 774
AcsI RAATTY 3 cut(s) 532, 563, 702
AcyI GRCGYC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 67
AgeI ACCGGT 1 cut(s) 333
AgsI TTSAA 3 cut(s) 230, 319, 446
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 6 cut(s) 145, 287, 587, 626, 672, 833
AluI AGCT 6 cut(s) 145, 287, 587, 626, 672, 833
Alw26I GTCTC 1 cut(s) 833
AlwI GGATC 4 cut(s) 52, 256, 322, 428
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 2 cut(s) 77, 774
ApeKI GCWGC 6 cut(s) 272, 411, 578, 801, 830, 833
ApoI RAATTY 3 cut(s) 532, 563, 702
AsiGI ACCGGT 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 88, 709
AsuHPI GGTGA 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 2 cut(s) 88, 709
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BarI GAAGNNNNNNTAC 2 cut(s) 159, 191
BbvI GCAGC 6 cut(s) 259, 423, 590, 813, 820, 842
BccI CCATC 5 cut(s) 99, 106, 396, 791, 823
BceAI ACGGC 2 cut(s) 45, 761
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 833
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 310
BisI GCNGC 6 cut(s) 273, 412, 579, 802, 831, 834
BlsI GCNGC 6 cut(s) 274, 413, 580, 803, 832, 835
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 2 cut(s) 88, 709
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 2 cut(s) 88, 709
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 4 cut(s) 160, 337, 367, 880
BpmI CTGGAG 2 cut(s) 138, 599
BpuEI CTTGAG 1 cut(s) 636
Bsa29I ATCGAT 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 749
BsaWI WCCGGW 2 cut(s) 333, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse118I RCCGGY 2 cut(s) 79, 333
Bse3DI GCAATG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 229
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 749
BseGI GGATG 4 cut(s) 12, 499, 802, 895
BseJI GATNNNNATC 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 3 cut(s) 408, 746, 828
BseX3I CGGCCG 2 cut(s) 77, 774
BseXI GCAGC 6 cut(s) 259, 423, 590, 813, 820, 842
BsgI GTGCAG 1 cut(s) 282
Bsh1285I CGRYCG 2 cut(s) 80, 777
BshFI GGCC 2 cut(s) 79, 776
BshTI ACCGGT 1 cut(s) 333
BshVI ATCGAT 1 cut(s) 400
BsiEI CGRYCG 2 cut(s) 80, 777
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 4 cut(s) 76, 80, 334, 558
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 833
BsmFI GGGAC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 2 cut(s) 79, 776
BsoBI CYCGRG 1 cut(s) 84
Bsp143I GATC 6 cut(s) 9, 26, 44, 248, 327, 433
Bsp19I CCATGG 1 cut(s) 749
BspACI CCGC 2 cut(s) 22, 576
BspANI GGCC 2 cut(s) 79, 776
BspCNI CTCAG 3 cut(s) 409, 745, 829
BspDI ATCGAT 1 cut(s) 400
BspHI TCATGA 2 cut(s) 430, 465
BspLI GGNNCC 1 cut(s) 89
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 4 cut(s) 52, 256, 322, 428
BspQI GCTCTTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 2 cut(s) 79, 333
BssAI RCCGGY 2 cut(s) 79, 333
BssECI CCNNGG 1 cut(s) 749
BssMI GATC 6 cut(s) 9, 26, 44, 248, 327, 433
BssNI GRCGYC 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 4 cut(s) 139, 324, 826, 879
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 807
BstC8I GCNNGC 4 cut(s) 81, 270, 638, 812
BstDEI CTNAG 3 cut(s) 417, 732, 837
BstDSI CCRYGG 1 cut(s) 749
BstF5I GGATG 4 cut(s) 12, 499, 802, 895
BstKTI GATC 6 cut(s) 12, 29, 47, 251, 330, 436
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 6 cut(s) 9, 26, 44, 248, 327, 433
BstMCI CGRYCG 2 cut(s) 80, 777
BstMWI GCNNNNNNNGC 4 cut(s) 170, 584, 782, 807
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 6 cut(s) 259, 423, 590, 813, 820, 842
BstZI CGGCCG 2 cut(s) 77, 774
Bsu15I ATCGAT 1 cut(s) 400
BsuRI GGCC 2 cut(s) 79, 776
BsuTUI ATCGAT 1 cut(s) 400
BtgI CCRYGG 1 cut(s) 749
BtsCI GGATG 4 cut(s) 12, 499, 802, 895
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 4 cut(s) 81, 270, 638, 812
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 2 cut(s) 430, 465
Cfr10I RCCGGY 2 cut(s) 79, 333
Cfr13I GGNCC 2 cut(s) 88, 709
ClaI ATCGAT 1 cut(s) 400
CseI GACGC 1 cut(s) 66
CspAI ACCGGT 1 cut(s) 333
CviAII CATG 7 cut(s) 238, 431, 466, 637, 641, 750, 856
DdeI CTNAG 3 cut(s) 417, 732, 837
DpnI GATC 6 cut(s) 11, 28, 46, 250, 329, 435
DpnII GATC 6 cut(s) 9, 26, 44, 248, 327, 433
EaeI YGGCCR 2 cut(s) 77, 774
EagI CGGCCG 2 cut(s) 77, 774
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 37
EclXI CGGCCG 2 cut(s) 77, 774
Eco130I CCWWGG 1 cut(s) 749
Eco47I GGWCC 2 cut(s) 88, 709
Eco52I CGGCCG 2 cut(s) 77, 774
Eco88I CYCGRG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 749
EcoT22I ATGCAT 2 cut(s) 642, 895
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 7 cut(s) 241, 434, 469, 640, 644, 753, 859
FaqI GGGAC 1 cut(s) 69
FatI CATG 7 cut(s) 237, 430, 465, 636, 640, 749, 855
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 6 cut(s) 273, 412, 579, 802, 831, 834
FokI GGATG 3 cut(s) 19, 506, 809
Fsp4HI GCNGC 6 cut(s) 273, 412, 579, 802, 831, 834
FspBI CTAG 1 cut(s) 221
GluI GCNGC 6 cut(s) 273, 412, 579, 802, 831, 834
GsuI CTGGAG 2 cut(s) 138, 599
HaeIII GGCC 2 cut(s) 79, 776
HapII CCGG 4 cut(s) 76, 80, 334, 558
HgaI GACGC 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 7 cut(s) 241, 434, 469, 640, 644, 753, 859
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 4 cut(s) 230, 419, 647, 848
HpaII CCGG 4 cut(s) 76, 80, 334, 558
HphI GGTGA 1 cut(s) 656
Hpy188I TCNGA 2 cut(s) 180, 235
Hpy188III TCNNGA 7 cut(s) 30, 246, 360, 431, 466, 616, 707
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 35, 200
HpyCH4III ACNGT 4 cut(s) 139, 324, 826, 879
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 584, 782, 807
HpyF3I CTNAG 3 cut(s) 417, 732, 837
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 7 cut(s) 241, 434, 469, 640, 644, 753, 859
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 6 cut(s) 9, 26, 44, 248, 327, 433
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 119, 142
Lsp1109I GCAGC 6 cut(s) 259, 423, 590, 813, 820, 842
LweI GCATC 4 cut(s) 160, 337, 367, 880
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 1 cut(s) 133
MalI GATC 6 cut(s) 11, 28, 46, 250, 329, 435
MboI GATC 6 cut(s) 9, 26, 44, 248, 327, 433
MboII GAAGA 1 cut(s) 167
MluCI AATT 7 cut(s) 105, 506, 532, 563, 702, 754, 819
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 3 cut(s) 12, 45, 203
Mph1103I ATGCAT 2 cut(s) 642, 895
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 2 cut(s) 606, 870
MslI CAYNNNNRTG 1 cut(s) 34
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 4 cut(s) 76, 80, 334, 558
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 4 cut(s) 170, 584, 782, 807
NaeI GCCGGC 1 cut(s) 81
NcoI CCATGG 1 cut(s) 749
NdeII GATC 6 cut(s) 9, 26, 44, 248, 327, 433
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 7 cut(s) 241, 434, 469, 640, 644, 753, 859
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 2 cut(s) 642, 895
NspI RCATGY 1 cut(s) 640
PaeI GCATGC 1 cut(s) 640
PagI TCATGA 2 cut(s) 430, 465
PaqCI CACCTGC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 180
PctI GAATGC 1 cut(s) 305
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 3 cut(s) 230, 647, 848
PinAI ACCGGT 1 cut(s) 333
PkrI GCNGC 6 cut(s) 274, 413, 580, 803, 832, 835
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 2 cut(s) 88, 709
PstNI CAGNNNCTG 1 cut(s) 836
PvuII CAGCTG 1 cut(s) 833
RseI CAYNNNNRTG 1 cut(s) 34
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 606, 870
SatI GCNGC 6 cut(s) 273, 412, 579, 802, 831, 834
Sau3AI GATC 6 cut(s) 9, 26, 44, 248, 327, 433
Sau96I GGNCC 2 cut(s) 88, 709
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 117
SfaNI GCATC 4 cut(s) 160, 337, 367, 880
SfcI CTRYAG 1 cut(s) 165
SgrAI CRCCGGYG 1 cut(s) 333
SinI GGWCC 2 cut(s) 88, 709
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 651
SmoI CTYRAG 1 cut(s) 651
SphI GCATGC 1 cut(s) 640
Sse9I AATT 7 cut(s) 105, 506, 532, 563, 702, 754, 819
SsiI CCGC 2 cut(s) 22, 576
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 4 cut(s) 139, 324, 826, 879
TaqI TCGA 3 cut(s) 50, 400, 668
TaqII GACCGA 1 cut(s) 875
TasI AATT 7 cut(s) 105, 506, 532, 563, 702, 754, 819
TfiI GAWTC 3 cut(s) 230, 647, 848
Tru1I TTAA 2 cut(s) 606, 870
Tru9I TTAA 2 cut(s) 606, 870
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 6 cut(s) 272, 411, 578, 801, 830, 833
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 6 cut(s) 213, 254, 297, 419, 482, 645
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 2 cut(s) 88, 709
XapI RAATTY 3 cut(s) 532, 563, 702
XceI RCATGY 1 cut(s) 640
XspI CTAG 1 cut(s) 221
Zsp2I ATGCAT 2 cut(s) 642, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.