RLG00000034941

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001971
Physical Location & Seq
Forward (+)
57399990 .. 57422220
22231 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034941

Sequence Viewer

Length: 6645 bp
ATGAGCGGCTACGAGAGCCGGAAACGGGACCGGAGAGACTCGTGGAGCTCCAAGAAGCAGCAGAAGCTCACGCGCACCGCCGACGAAGACCTCGAGTCAAAGCTCGGATTCGATCTATTCTCCGAAGGTGAAACCAGGCTCGGCTGGTTACTCACTTTCGCAACCTCATCTTGTGAAGATCAAGAGACCCACAAGGTTTATAGCTGCGTTGATCTGTATTTCGTTACACAGGATGGCTCGGCTTTCAAGTCAAAATACAAGTTTCGGCCTTATTTCTATGCAGCCACAAAGGAAAAAATGGAAATGGATGTTGAAGCATATTTGAGACGGCGCTATGAAAGTCGTATTGCTGATATCGAAATAGTGGCGAAAGAAGATCTTGATCTTAAAAACCATTTGTCTGGCCTACACAAGTCCTATTTGAAGTTATCATTTGATACTGTTCAACAATTAATGAATGTCAAGAGTGACCTATTGCAAGTCGTCGAAAGAAACCACACAAAATTTGATGCTGCCGAAGCTTATGAGTCAATACTGCTAGGGAAACGAGAACAAAGGCCTCAAGATTTCCTAGATTGTATTGTTGATCTTCGTGAATACGATGTTCCCTACCATGTTCGCTTTGCCATTGACAATGATGTGCGATGTGGCCAGTGGTACAATGTTAGTGTATCCAGTACGGGTGTTATGCTTGAGAGGAGGACAGATCTTCTGCAGCATGCTGAAGTTCATGTTTGTGCATTTGATATTGAGACAACTAAGCTGCCCTTAAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGATGGACAAGGGTACCTGATTATTAATAGAGAGTGTGTTGGAGCTGATATAGAGTATTTGGAGTACACTCCAAAAGAAGAATTTGTAGGGCATTTCAAGGTGACAAATGTGGAAAATGAGAGAGAGCTTCTAAGACAGTGGTTTGCCCATATGCGGGAGGTGAAGCCTGGAATCTATGTCACATACAATGGTGATTTTTTCGATTGGCCTTTCCTGGAAACGAGAGCTGCACATCATGGGTTCAAAATGAATGATGAGGTTGGATTTTTATGTGACAAGAATCAGGGGGAATGTAGAGCTAAATTTTCCTGTCATCTTGATTGCTTTGCTTGGGTCAAACGTGATAGTTATCTGCCCCAAGGGAGCCAGGGCCTTAAGGCTGTTACGAAGGCAAAGTTGGGTTATGATCCAATAGAGGTGAATCCTGAGGATATGGTTCGCTTTGCGAAAGAAGAACCTCAGAGGATGGCATCCTATTCTGTTTCTGATGCTGTTGCAACCTATTATTTGTACATGACCTATGTTCATCCATTTATCTTCTCTCTTGCAACTATTATTCCAATGTCTCCGGATGAGGTTTTGCGCAAGGGTAGTGGAACCCTTTGTGAAATGCTTTTAATGGTCCAGGCATACAAGGCAAATGTCATCTGTCCTAACAAGCACCAATCTGACCCGGAAAAGTTCTATAAAAACCACCTTTTGGAAAGTGAGACTTACATAGGTGGCCATGTGGAATGCCTTGAAAGTGGTGTTTTCAGATCTGATCTTCCAACTAATTTTAGGCTTGATCCAGCTGCTTACGAGCAATTGATCAACAACCTTGATCGAGATCTTCAATATGCTATCAGAGTAGAGGGAAAGATGGACTTGGATTCTGTGTCCAATTATGATGAAGTGAAGAGTTCTATTCTGGAAAAGCTTGTGAGGTTGCGAGATGAACCTATACGAGAAGAATGCCCACTTATTTATCACCTGGATGTAGCTGCAATGTATCCCAACATTATTTTAACAAACAGGCTTCAGCCCACATCAATTGTTACAGATGAGGTCTGCACTGCTTGTGATTTCAACCGTCCAGGAAAGACATGTCTACGAAAGCTGGAATGGGTTTGGCGTGGAGAAATATTCATGGCCAAAAGAAGCGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTCTGTTGACGGTGCCACTTCCAAGCAGTCAAGGTCTTTTCTTGAATTGCCTAAAATGGAACAACAAACAAGGCTCAAAGAACGTCTAAAGAAATACTGTCAGAAGGCATATAAACGCGTAGTAGACAAGCCAGTCACTGAACTTCGGGAAGCAGAGATTTGCATGAGGGAAAATTCATTCTATATTGACACAGTTCGCAGCTTTCGAGATAGAAGGTACGAGTATAAAGGGCTTAATAAGGTTTGGAAGGGCAAGTTGTCTGAAGCAAAGGCTAGTAAAAATTCTATAAAAATCCAGGAAGCACAAAATATGGTGGTGCTTTATGATTCCCTACAACTTGCACATAAGTGTATACTTAACTCCTTTTATGGATATGTCATGCGCAAGGGTGCAAGATGGTATTCAATGGAAATGGCTGGAGTAGTTACATATACTGGAGCTAAAATTATTCAGAATGCTCGGTTGCTAGTTGAAAAAATTGGAAAGCCTCTTGAGTTAGATACAGATGGTATTTGGTGTGTGCTTCCTGGATCTTTTCCGGAGAACTTTACTTTCAAAACAAAAGACTTGAAGAAGAAGCTAACTATCTCATATCCATGCGTCATGCTTAATGTTGATGTGGCGAGAAACAATACAAACGATCAATACCAGACTCTTACAGATCCGATCGAGAAAACTTATGCAACTCATAGTGAATGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTATTCCTGCTTCTAAGGAAGAAGGAATTCTAATTAAGAAGCGCTATGCTGTCTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTTGAGATCAAACGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAGCTTTTCGATAAGTTCCTTCACGGTTCTACATTAGAGGAATGCTATTCAGCAGTTGCTTCTGTTGCCAACCGCTGGCTTGATCTTCTTGATAATCAAGGAAAGGATATAGCAGACAGTGAGTTGCTTGATTATATATCAGAATCAAGCACCATGAGCAAGTCTTTGGCGGAATATGGAAGCCAAAAGTCATGTGCAGTAACCACTGCAAGGCGTCTTGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTACGTTGCCAGTATATAGTTGCGTGTGAACCAAAGGGAACACCTGTCAGTGAGCGTGCTGTTCCAGTTGCAATATTCGAAACAGATGCTGAAATTATGAAGTTTTATGTACGGAAGTGGTGCAAGGTCTCCGATGTTGACATCAGATCCATTATAGACTGGTCCTATTACAAACAACGTCTAGGTTCAGCAATTCAGAAAATCGTCACAATTCCTGCTGCAATGCAAAAGGTTGCAAATCCTGTCCCTAGGGTAGTCCATCCTGATTGGCTACATAAGAAGGTTCGTGAGAAGGAAGATAAATTTCGACAACGCAAATTGGTTGATATCTTCAGGTCATTGAATGGGGATCATTTGGTGCAAAACAATGATGCTATTGGCTCTAATCATGTGGTAGATGAAGATATTGTTGAAGATTTGGAAGACTTCAAATGCAAAAGCAGTTCCATAAGTAGACCGAGACCAATTGTTCACTGTTATGAAGTTAATAACCAGAAAAATTCAGTCAAGGCAAATGGTAAAGGAGGTCCAGAGCAACAACAAACTAATCATATAGAAAATGCTCATCAAGAGCAAACTGCTGTCACTCAAGAAGAAATTGACAAAAATGTAGATTATCAAGGATGGCTTGCAGTAAAGAAAAGAAAGTGGAAAGATACTCTTGACAAGAGGAAGAAACAACGATTGAATAATGCAAGGTCTGCTCATAATGCTAATGACAAGATGCTTGGTGGTGGGAGAAATCTCAAAGGGGCTCAGGGGAAAATAGGTGTGAATTCATATTTTAGGAGTCATGAAGATGCCCTAACTCGCTGTCATTGGCAGATATTACAACTAGTTCCAAGTTCTGAGAGTGGCCAAATTTTGGCGTGGCTTGTTGTTGAAGGAATGATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATCAATTCTACATCACCTATAAAAGAAGAATTGAAAGAAGAATTACCAGGGAAGTATGTCAACAAGACTCTTCCTCACGGAAGACATAGTTATTACCTATATGAGGTTTCAGTTGATGAGAAGAGGTTTAGGACACTGAGAAAAAAGCTTGCGGCTCTTTTCGCTGATCCAGATATTGAGGGAATATATGAAACAAAGGTGCCGTCGGAGTTTAATGTAATCACTCAGATTGGTTGTGTCTGTAAATTGGACAAAAAAGCTCAGGGAGATGCCAAAAATGGCTGGAGTGTTAGTGCACTGGACATGAAGAATACGACAGAGTGCTCTTATCTGGCCCAATCCATCGCTTTCTTTTATTTGTATCACAGCATCTCTGAAGGGCGCGCAATATTTGTTGCTTATTTTCCTGCATCAAGAACAATAATTGTTGTGGTGGTGAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAAAAACAATTCCGCGACGCTTGCCAGGCATTATCACTTCAGCTGCCTCCTAGGAATGGAATCATTATCAAGGTAGACTACGTTGGGTATGTAAAGGATGCTGAAAAGATTTTGCACCAAGCAATTAATGAGCAAAGACATGATCATGGACCTACAATTGCTGTTATTGAATGCCCAAATGTTCAGTTAATGAAGTCAGGTATACGAGCTCTTGATGATTTTCCATGTGTGAGCATTCCGTGTAATGCTCGAGACAGTCAATATCAGGTCCTTGGGTGGCAACAAGTGGCTGCAAAAATTGGCATGCAACGATGTGCTGCATCATCGCAGTGGTTGATTGATAGAATTTCTCTCTCAAGATATGCACATGTACCATTGGGCAATTTTGAAGTTGACTCCCTTATGCATACAGCTGATATCTTCTTTTCAAGAGCATTACGTGATAGCCAGCAGGTACTTTGGATATCTGATGATGGTATTCCAGATATAGGAGGCATTAACGAAGAAGGTGGATTTTTTGCAGATGAGGTCCATCAACCATTTGTAATTACTAAACCTGGAGCATATAGAAAGGTCACTGTGGAGCTAAAGATACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAATGAAATGGAAGGTGGTGCTTTGCTAGGATTTGAATCAGACATGAACTCCGGATCACATACTTCTAATATGCAGGCTGCCTTTGATGAAGCTACATCATCTGCACCTGCATTCCGTGTTTTGAAACAGTTGATCCACAGGTGTCTTGCAGATGCACATACATCTGGAAATGTGTATGCTGATGCACTACTGCAACATCTGTATCGATGGCTTTGCAGCCCCCAGTCTAAACTTCATGATCCAGCACTTCAACGCCTACTTCACAAGGTCATGAAAAAGGTGCTTTTGATTTTATTGGATGAGTTCCGCAAATTGGGTGCAAATATCATATTTGCAAATTTCTATAAGGTTATTATAGACACAGGAAAATTTGATATATCCGCAGCCAAAGCTTATTGTGATAGTGTGCTTAAAACTCTCCAGAAAAGAGAACTATTTGAGTGGATTGAGCTTGAACCATTGCACTACTGGCATTCTTTGCTTTTCATGGATCAGTATAATTATGGTGGAATTCCGGTTAGAGCTGATGAAAGCATGCATGGTGGATCTCAAGTGGATATTGTATCGAGCTGGAACATTGCTGAATACTTACCCAAGAAAATTCAGGACCATTTTATTTTTATTGTTTCTCAGTTTTTGTATATTCCCTGGAATTATGCACAGAAGCAGTCGGCTGTTAGAACGACTTTACAGGTTGGCAGCAGTTGCACACCATCAATTACGGTTGCAGCTGCTGAGACTTTGGAATCTCACATGACCGATTTTCTTAAAGGACAGATCAGCTCGTACTTTACTGAAAAGCTTCTGGGAATTGTTCGTGATACAATACTTCATATGAAGGAACTGAACAAGTCTGAGAATGACTATCGGACATCTCCTGGGCTGCCACAACTTGCTGGTAATATCCACAAAGGAGATGCGGCACTTGAGTTCATTAAGAATGTCTGCGGTGTTTTGCTCATTGACCAAAGTGTTGAGCACGACATTATGGTAATGAGAAGTAATCTATTAAAATATATGCATGTGAGGGAGTCTGCTCCTGAAGCTCAGTTTTGTGATCCCTGCCCATCCTTGTTCTTACGAAATGTCATTTGCAGCTACTGCAATGACTGTAGAGACCTAGACATCTGCCGTGATTCAGCTCTGCTAGCTGGGGAGTGGCGCTGTGCTGTGCCCCAATGCGGGCAGCCTTATGACAGAGAGATGATGGAGAATACCCTTCTTCAGATTGTACAGCAGAGAGAGCGTCTCTACCACCTACAGGATCTGGTGTGTCTCAGGTGTAACCAAGTGAAAGCTGCACATCTAGCAGAACAATGTGGATGTGCCGGCTCTTTTAGCTGTAAGGAAGATGCAGCTGAATTTTGTGAAAAGATGCAGTTAATCTTGAAAATTGCAATTCATCAGAAATTTCAATTTCTACAAGAGTGCACTGAGTGGATTTTAGAAGTTGAGAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2215

Amino Acids

253.61

Weight (kDa)

6.48

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 196 - 402 5.1e-56 DNA polymerase family B, exonuclease domain
DNA_pol_B_exo2 PF10108 309 - 449 7.9e-06 Predicted 3'-5' exonuclease related to the exonuclease domain of PolB
DNA_pol_B PF00136 749 - 1073 1.8e-18 DNA polymerase family B
POL2_thumb PF22634 948 - 1122 2.9e-78 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1532 - 1898 4.7e-113 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2075 - 2141 9.1e-20 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2147 - 2203 6.4e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5296
AasI GACNNNNNNGTC 1 cut(s) 2132
Acc16I TGCGCA 2 cut(s) 1406, 2384
Acc36I ACCTGC 2 cut(s) 4987, 5296
Acc65I GGTACC 1 cut(s) 834
AccB1I GGYRCC 3 cut(s) 834, 2012, 4267
AccB7I CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
AccII CGCG 4 cut(s) 73, 2118, 4452, 4560
AccIII TCCGGA 3 cut(s) 1390, 2539, 5231
AcoI YGGCCR 4 cut(s) 649, 1546, 1953, 3994
AcuI CTGAAG 9 cut(s) 744, 1826, 2283, 2876, 3454, 4464, 4568, 6243, 6389
AcyI GRCGYC 1 cut(s) 3094
AdeI CACNNNGTG 1 cut(s) 6618
AfeI AGCGCT 1 cut(s) 2783
AflII CTTAAG 2 cut(s) 1196, 4034
AflIII ACRYGT 3 cut(s) 1907, 2116, 4912
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 3973
AjuI GAANNNNNNNTTGG 2 cut(s) 1202, 1234
AleI CACNNNNGTG 1 cut(s) 2347
Alw21I GWGCWC 6 cut(s) 50, 4366, 4394, 4756, 6162, 6614
Alw44I GTGCAC 2 cut(s) 4362, 6610
AlwNI CAGNNNCTG 6 cut(s) 785, 2138, 3227, 5915, 6282, 6448
Ama87I CYCGRG 2 cut(s) 92, 4794
Aor13HI TCCGGA 3 cut(s) 1390, 2539, 5231
Aor51HI AGCGCT 1 cut(s) 2783
ApaLI GTGCAC 2 cut(s) 4362, 6610
ArsI GACNNNNNNTTYG 2 cut(s) 4256, 4288
AseI ATTAAT 3 cut(s) 452, 846, 4671
Asp700I GAANNNNTTC 2 cut(s) 2766, 5982
Asp718I GGTACC 1 cut(s) 834
AspA2I CCTAGG 2 cut(s) 3386, 4595
AspLEI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
AsuC2I CCSGG 1 cut(s) 1496
AsuII TTCGAA 1 cut(s) 3216
AsuNHI GCTAGC 1 cut(s) 6328
AvaI CYCGRG 2 cut(s) 92, 4794
AvrII CCTAGG 2 cut(s) 3386, 4595
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 3 cut(s) 4366, 6357, 6614
BaeI ACNNNNGTAYC 6 cut(s) 818, 851, 3108, 3141, 5366, 5399
BalI TGGCCA 4 cut(s) 651, 1548, 1955, 3996
BanI GGYRCC 3 cut(s) 834, 2012, 4267
BanII GRGCYC 3 cut(s) 50, 3895, 4756
BarI GAAGNNNNNNTAC 2 cut(s) 5145, 5177
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 3 cut(s) 93, 3567, 4156
Bbv12I GWGCWC 6 cut(s) 50, 4366, 4394, 4756, 6162, 6614
BceAI ACGGC 3 cut(s) 344, 4255, 6297
BcgI CGANNNNNNTGC 6 cut(s) 1758, 1792, 3206, 3240, 5376, 5410
BciVI GTATCC 2 cut(s) 682, 1824
BclI TGATCA 2 cut(s) 1632, 4687
BcnI CCSGG 1 cut(s) 1496
BcuI ACTAGT 1 cut(s) 3973
BfmI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
BfoI RGCGCY 3 cut(s) 334, 2785, 6346
BfrI CTTAAG 2 cut(s) 1196, 4034
BfuAI ACCTGC 2 cut(s) 4987, 5296
BfuI GTATCC 2 cut(s) 682, 1824
BglII AGATCT 4 cut(s) 376, 706, 1580, 1651
BlnI CCTAGG 2 cut(s) 3386, 4595
BlpI GCTNAGC 1 cut(s) 2880
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 2 cut(s) 92, 4794
BmiI GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4269
BmrI ACTGGG 1 cut(s) 5398
BmtI GCTAGC 1 cut(s) 6332
BmuI ACTGGG 1 cut(s) 5398
BpiI GAAGAC 3 cut(s) 93, 3567, 4156
BplI GAGNNNNNCTC 2 cut(s) 6414, 6446
BpmI CTGGAG 5 cut(s) 2439, 2457, 4372, 5124, 5585
Bpu10I CCTNAGC 2 cut(s) 3894, 4329
Bpu1102I GCTNAGC 1 cut(s) 2880
Bpu14I TTCGAA 1 cut(s) 3216
BpuEI CTTGAG 7 cut(s) 546, 713, 2513, 3711, 4885, 5715, 6128
BpuMI CCSGG 1 cut(s) 1496
Bsa29I ATCGAT 1 cut(s) 5386
BsaAI YACGTR 1 cut(s) 4985
BsaBI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BsaHI GRCGYC 1 cut(s) 3094
BsaI GGTCTC 4 cut(s) 179, 3271, 3592, 6291
BsaJI CCNNGG 8 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828, 6059
BsaWI WCCGGW 5 cut(s) 30, 1390, 2539, 5231, 5695
BsaXI ACNNNNNCTCC 4 cut(s) 4345, 4375, 5213, 5243
Bse118I RCCGGY 1 cut(s) 6509
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
Bse8I GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BseAI TCCGGA 3 cut(s) 1390, 2539, 5231
BseCI ATCGAT 1 cut(s) 5386
BseDI CCNNGG 8 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828, 6059
BseJI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5215
BseMI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
BsePI GCGCGC 1 cut(s) 4450
BseRI GAGGAG 1 cut(s) 712
BseSI GKGCMC 3 cut(s) 4366, 6357, 6614
BseYI CCCAGC 1 cut(s) 6332
BsgI GTGCAG 5 cut(s) 1035, 1858, 3096, 5268, 6465
Bsh1236I CGCG 4 cut(s) 73, 2118, 4452, 4560
Bsh1285I CGRYCG 1 cut(s) 2670
BshNI GGYRCC 3 cut(s) 834, 2012, 4267
BshVI ATCGAT 1 cut(s) 5386
BsiEI CGRYCG 1 cut(s) 2670
BsiHKAI GWGCWC 6 cut(s) 50, 4366, 4394, 4756, 6162, 6614
BsiHKCI CYCGRG 2 cut(s) 92, 4794
BsiSI CCGG 8 cut(s) 19, 31, 1391, 1496, 2540, 5232, 5696, 6510
BslFI GGGAC 3 cut(s) 41, 2821, 3368
BsmBI CGTCTC 2 cut(s) 319, 6434
BsmFI GGGAC 3 cut(s) 41, 2821, 3368
BsmI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
Bso31I GGTCTC 4 cut(s) 179, 3271, 3592, 6291
BsoBI CYCGRG 2 cut(s) 92, 4794
Bsp119I TTCGAA 1 cut(s) 3216
Bsp1286I GDGCHC 8 cut(s) 50, 3895, 4366, 4394, 4756, 6162, 6357, 6614
Bsp13I TCCGGA 3 cut(s) 1390, 2539, 5231
Bsp1407I TGTACA 2 cut(s) 1332, 6412
Bsp1720I GCTNAGC 1 cut(s) 2880
BspDI ATCGAT 1 cut(s) 5386
BspEI TCCGGA 3 cut(s) 1390, 2539, 5231
BspFNI CGCG 4 cut(s) 73, 2118, 4452, 4560
BspHI TCATGA 3 cut(s) 3931, 5416, 5451
BspLI GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4269
BspMAI CTGCAG 1 cut(s) 717
BspMI ACCTGC 2 cut(s) 4987, 5296
BspOI GCTAGC 1 cut(s) 6332
BspQI GCTCTTC 1 cut(s) 5166
BspT104I TTCGAA 1 cut(s) 3216
BspT107I GGYRCC 3 cut(s) 834, 2012, 4267
BspTI CTTAAG 2 cut(s) 1196, 4034
BspTNI GGTCTC 4 cut(s) 179, 3271, 3592, 6291
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 6 cut(s) 1815, 2742, 3366, 5639, 5757, 6292
BsrFI RCCGGY 1 cut(s) 6509
BsrGI TGTACA 2 cut(s) 1332, 6412
BssAI RCCGGY 1 cut(s) 6509
BssECI CCNNGG 8 cut(s) 1180, 1189, 3386, 4115, 4595, 4816, 5828, 6059
BssHII GCGCGC 1 cut(s) 4450
BssNAI GTATAC 2 cut(s) 2354, 4748
BssNI GRCGYC 1 cut(s) 3094
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Bst1107I GTATAC 2 cut(s) 2354, 4748
Bst2BI CACGAG 1 cut(s) 40
Bst6I CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
BstACI GRCGYC 1 cut(s) 3094
BstAFI CTTAAG 2 cut(s) 1196, 4034
BstAPI GCANNNNNTGC 4 cut(s) 3839, 5659, 5886, 6282
BstAUI TGTACA 2 cut(s) 1332, 6412
BstBAI YACGTR 1 cut(s) 4985
BstBI TTCGAA 1 cut(s) 3216
BstENI CCTNNNNNAGG 1 cut(s) 4169
BstFNI CGCG 4 cut(s) 73, 2118, 4452, 4560
BstH2I RGCGCY 3 cut(s) 334, 2785, 6346
BstHHI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
BstMCI CGRYCG 1 cut(s) 2670
BstNSI RCATGY 6 cut(s) 722, 1911, 4852, 4916, 5719, 6206
BstSFI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
BstSLI GKGCMC 3 cut(s) 4366, 6357, 6614
BstUI CGCG 4 cut(s) 73, 2118, 4452, 4560
BstV2I GAAGAC 3 cut(s) 93, 3567, 4156
BstX2I RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5726, 6445
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5726, 6445
BstZ17I GTATAC 2 cut(s) 2354, 4748
Bsu15I ATCGAT 1 cut(s) 5386
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 2 cut(s) 682, 1824
BsuTUI ATCGAT 1 cut(s) 5386
BtgZI GCGATG 3 cut(s) 658, 4396, 4854
BtsI GCAGTG 3 cut(s) 1875, 3084, 4880
BveI ACCTGC 2 cut(s) 4987, 5296
CaiI CAGNNNCTG 6 cut(s) 785, 2138, 3227, 5915, 6282, 6448
CciI TCATGA 3 cut(s) 3931, 5416, 5451
CfoI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
Cfr10I RCCGGY 1 cut(s) 6509
ClaI ATCGAT 1 cut(s) 5386
CseI GACGC 4 cut(s) 2590, 3083, 4571, 6416
CspCI CAANNNNNGTGG 4 cut(s) 1396, 1431, 1837, 1872
DraIII CACNNNGTG 1 cut(s) 6618
DrdI GACNNNNNNGTC 1 cut(s) 2132
DriI GACNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 2132
EaeI YGGCCR 4 cut(s) 649, 1546, 1953, 3994
Eam1104I CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 4 cut(s) 1715, 4143, 4184, 5166
EciI GGCGGA 1 cut(s) 3065
Ecl136II GAGCTC 2 cut(s) 48, 4754
Eco130I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 3 cut(s) 50, 3895, 4756
Eco31I GGTCTC 4 cut(s) 179, 3271, 3592, 6291
Eco32I GATATC 4 cut(s) 355, 3466, 4963, 5010
Eco47III AGCGCT 1 cut(s) 2783
Eco53kI GAGCTC 2 cut(s) 48, 4754
Eco57I CTGAAG 9 cut(s) 744, 1826, 2283, 2876, 3454, 4464, 4568, 6243, 6389
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 2 cut(s) 92, 4794
EcoICRI GAGCTC 2 cut(s) 48, 4754
EcoNI CCTNNNNNAGG 1 cut(s) 4169
EcoO109I RGGNCCY 3 cut(s) 1192, 2808, 4813
EcoRI GAATTC 3 cut(s) 2766, 3913, 5691
EcoRV GATATC 4 cut(s) 355, 3466, 4963, 5010
EcoT14I CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
EcoT22I ATGCAT 3 cut(s) 4953, 5721, 6204
EcoT38I GRGCYC 3 cut(s) 50, 3895, 4756
ErhI CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
Esp3I CGTCTC 2 cut(s) 319, 6434
FaqI GGGAC 3 cut(s) 41, 2821, 3368
FauI CCCGC 2 cut(s) 969, 6356
FauNDI CATATG 2 cut(s) 972, 6015
FbaI TGATCA 2 cut(s) 1632, 4687
FblI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
FriOI GRGCYC 3 cut(s) 50, 3895, 4756
FspI TGCGCA 2 cut(s) 1406, 2384
GlaI GCGC 8 cut(s) 74, 332, 1406, 2384, 2783, 4451, 4453, 6344
GsaI CCCAGC 1 cut(s) 6336
GsuI CTGGAG 5 cut(s) 2439, 2457, 4372, 5124, 5585
HaeII RGCGCY 3 cut(s) 334, 2785, 6346
HapII CCGG 8 cut(s) 19, 31, 1391, 1496, 2540, 5232, 5696, 6510
HgaI GACGC 4 cut(s) 2590, 3083, 4571, 6416
HhaI GCGC 8 cut(s) 75, 333, 1407, 2385, 2784, 4452, 4454, 6345
Hin1I GRCGYC 1 cut(s) 3094
Hin6I GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
HinP1I GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
HincII GTYRAC 4 cut(s) 2008, 3277, 4129, 4939
HindII GTYRAC 4 cut(s) 2008, 3277, 4129, 4939
HindIII AAGCTT 5 cut(s) 519, 1739, 4214, 5571, 5981
HpaII CCGG 8 cut(s) 19, 31, 1391, 1496, 2540, 5232, 5696, 6510
Hpy99I CGWCG 4 cut(s) 86, 488, 4276, 4565
HpyCH4IV ACGT 7 cut(s) 1162, 2083, 2842, 3142, 3316, 4626, 4984
HpySE526I ACGT 7 cut(s) 1162, 2083, 2842, 3142, 3316, 4626, 4984
Hsp92I GRCGYC 1 cut(s) 3094
HspAI GCGC 8 cut(s) 73, 331, 1405, 2383, 2782, 4450, 4452, 6343
KflI GGGWCCC 1 cut(s) 2808
Kpn2I TCCGGA 3 cut(s) 1390, 2539, 5231
KpnI GGTACC 1 cut(s) 838
KroI GCCGGC 1 cut(s) 6509
KroNI GCCGGC 1 cut(s) 6511
Ksp22I TGATCA 2 cut(s) 1632, 4687
LguI GCTCTTC 1 cut(s) 5166
LmnI GCTCC 8 cut(s) 45, 53, 863, 1185, 2438, 5105, 5128, 6223
MaeII ACGT 7 cut(s) 1162, 2083, 2842, 3142, 3316, 4626, 4984
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 5 cut(s) 1628, 1854, 2703, 3603, 4701
MflI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5726, 6445
MhlI GDGCHC 8 cut(s) 50, 3895, 4366, 4394, 4756, 6162, 6357, 6614
MlsI TGGCCA 4 cut(s) 651, 1548, 1955, 3996
MluI ACGCGT 1 cut(s) 2116
MluNI TGGCCA 4 cut(s) 651, 1548, 1955, 3996
MmeI TCCRAC 4 cut(s) 841, 1063, 1616, 4254
Mox20I TGGCCA 4 cut(s) 651, 1548, 1955, 3996
Mph1103I ATGCAT 3 cut(s) 4953, 5721, 6204
MroI TCCGGA 3 cut(s) 1390, 2539, 5231
MroNI GCCGGC 1 cut(s) 6509
MroXI GAANNNNTTC 2 cut(s) 2766, 5982
MscI TGGCCA 4 cut(s) 651, 1548, 1955, 3996
MslI CAYNNNNRTG 8 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4689, 4873
Msp20I TGGCCA 4 cut(s) 651, 1548, 1955, 3996
MspA1I CMGCKG 6 cut(s) 1616, 2955, 4588, 4958, 5912, 6539
MspCI CTTAAG 2 cut(s) 1196, 4034
MspI CCGG 8 cut(s) 19, 31, 1391, 1496, 2540, 5232, 5696, 6510
MunI CAATTG 5 cut(s) 1628, 1854, 2703, 3603, 4701
Mva1269I GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
MvnI CGCG 4 cut(s) 73, 2118, 4452, 4560
NaeI GCCGGC 1 cut(s) 6511
NciI CCSGG 1 cut(s) 1496
NdeI CATATG 2 cut(s) 972, 6015
NgoMIV GCCGGC 1 cut(s) 6509
NheI GCTAGC 1 cut(s) 6328
NlaIV GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4269
NmeAIII GCCGAG 2 cut(s) 120, 218
NmuCI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
NsbI TGCGCA 2 cut(s) 1406, 2384
NsiI ATGCAT 3 cut(s) 4953, 5721, 6204
NspI RCATGY 6 cut(s) 722, 1911, 4852, 4916, 5719, 6206
NspV TTCGAA 1 cut(s) 3216
OliI CACNNNNGTG 1 cut(s) 2347
PaeI GCATGC 3 cut(s) 722, 4852, 5719
PaeR7I CTCGAG 2 cut(s) 92, 4794
PagI TCATGA 3 cut(s) 3931, 5416, 5451
PaqCI CACCTGC 1 cut(s) 5296
PauI GCGCGC 1 cut(s) 4450
PceI AGGCCT 1 cut(s) 559
PciI ACATGT 2 cut(s) 1907, 4912
PciSI GCTCTTC 1 cut(s) 5166
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 2203
PctI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4721, 4779, 5291, 5653
PdiI GCCGGC 1 cut(s) 6511
PdmI GAANNNNTTC 2 cut(s) 2766, 5982
PflMI CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
PfoI TCCNGGA 4 cut(s) 1035, 1897, 2295, 2527
Ple19I CGATCG 1 cut(s) 2670
Ppu21I YACGTR 1 cut(s) 4985
PpuMI RGGWCCY 2 cut(s) 2808, 4813
PscI ACATGT 2 cut(s) 1907, 4912
PshBI ATTAAT 3 cut(s) 452, 846, 4671
Psp124BI GAGCTC 2 cut(s) 50, 4756
Psp5II RGGWCCY 2 cut(s) 2808, 4813
PspFI CCCAGC 1 cut(s) 6332
PspN4I GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4269
PspPPI RGGWCCY 2 cut(s) 2808, 4813
PspXI VCTCGAGB 1 cut(s) 92
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 6 cut(s) 785, 2138, 3227, 5915, 6282, 6448
PsuI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5726, 6445
PteI GCGCGC 1 cut(s) 4450
PvuI CGATCG 1 cut(s) 2670
PvuII CAGCTG 5 cut(s) 1616, 4588, 4958, 5912, 6539
RseI CAYNNNNRTG 8 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4689, 4873
SacI GAGCTC 2 cut(s) 50, 4756
SapI GCTCTTC 1 cut(s) 5166
SduI GDGCHC 8 cut(s) 50, 3895, 4366, 4394, 4756, 6162, 6357, 6614
SfcI CTRYAG 4 cut(s) 713, 5151, 6292, 6440
Sfr274I CTCGAG 2 cut(s) 92, 4794
SfuI TTCGAA 1 cut(s) 3216
SlaI CTCGAG 2 cut(s) 92, 4794
SmiMI CAYNNNNRTG 8 cut(s) 735, 1797, 2347, 2596, 3615, 3936, 4689, 4873
SpeI ACTAGT 1 cut(s) 3973
SphI GCATGC 3 cut(s) 722, 4852, 5719
SseBI AGGCCT 1 cut(s) 559
SspI AATATT 3 cut(s) 1947, 3213, 4458
SstI GAGCTC 2 cut(s) 50, 4756
StuI AGGCCT 1 cut(s) 559
StyI CCWWGG 4 cut(s) 1180, 3386, 4595, 4816
TaiI ACGT 7 cut(s) 1165, 2086, 2845, 3145, 3319, 4629, 4987
TaqII GACCGA 2 cut(s) 3610, 5954
TatI WGTACW 3 cut(s) 885, 1332, 6412
TauI GCSGC 3 cut(s) 9, 4223, 6104
TseFI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
Tsp45I GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3721, 4530, 5119
TspGWI ACGGA 4 cut(s) 3265, 4161, 4773, 5285
Van91I CCANNNNNTGG 3 cut(s) 1522, 2955, 4003
Vha464I CTTAAG 2 cut(s) 1196, 4034
VneI GTGCAC 2 cut(s) 4362, 6610
VspI ATTAAT 3 cut(s) 452, 846, 4671
XagI CCTNNNNNAGG 1 cut(s) 4169
XceI RCATGY 6 cut(s) 722, 1911, 4852, 4916, 5719, 6206
XcmI CCANNNNNNNNNTGG 1 cut(s) 5646
XhoI CTCGAG 2 cut(s) 92, 4794
XmaJI CCTAGG 2 cut(s) 3386, 4595
XmiI GTMKAC 6 cut(s) 1912, 2124, 2353, 3592, 4620, 4747
XmnI GAANNNNTTC 2 cut(s) 2766, 5982
Zsp2I ATGCAT 3 cut(s) 4953, 5721, 6204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.