RchiOBHm_Chr5g0054631

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001971
Physical Location & Seq
Forward (+)
57268290 .. 57291119
22830 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33174

Sequence Viewer

Length: 6642 bp
ATGAGCGGCTACGAGAGCCGGAAACGGGACCGGAGAGACGCGTGGAGCTCCAAGAAGCAGCAGAAGCTCACGCGCACCGCCGACGAAGACCTCGAGTCAAAGCTCGGATTCGATCTATTCTCCGAAGGTGAAACTAGGCTCGGCTGGTTACTCACTTTCGCAACGTCATCCTGTGAAGATCAAGACACCCATAAAGTTTATAGCTGCGTCGATCTGTATTTCGTTACACAGGATGGCTCTGCTTTCAAGTCAAAATATAAGTTTCGCCCTTATTTCTATGCAGCCACAAAGGAAAAAATGGAAATGGATGTTGAAGCATATTTGAGACGGCGCTATGAAAGTCGTATTGCTGATATCGAAATAGTGGCGAAAGAAGATCTTGATCTTAAAAACCATTTGTCTGGCCTACACAAGTCCTATTTGAAGTTATCATTTGATACTGTTCAACAATTAATGAATGTCAAGAGTGACCTATTGCAAGTCGTCGAAAGAAACCACACAAAATTTGATGCTGCTGAAGCTTATGAGTCAATACTGCTAGGGAAACGGGAACAAAGGCCTCAAGATTTCCTAGATTGTATTGTTGATCTTCGTGAATACGATGTTCCCTACCATGTTCGCTTCGCTATTGACAATGATGTGCGATGTGGCCAGTGGTACAATGTTAGTGTATCCAGTGCGGGTGTTATGCTTGAGAGGAGGACAGATCTTCTGCAGCATGCTGAAGTTCATGTTTGTGCATTTGATATTGAGACAACTAAGCTGCCCTTAAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGACGGACAAGGGTACCTGATTATTAATAGAGAGTGTGTTGGAGCTGATATAGAGTATTTGGAGTACACTCCAAAAGAAGAATTTGTAGGGCATTTCAAGGTGACAAATGTGGAAAATGAGAGAGAGCTTCTAAGACAGTGGTTTGCCCATATGCGGGAGGTGAAGCCTGGAATCTATGTCACATACAATGGTGATTTTTTCGATTGGCCTTTCCTGGAAACGAGAGCTGCACATCATGGGTTCAAAATGAATGATGAGGTTGGATTTTTATGTGACAAGAATCAGGGGGAATGTAGAGCTAAATTTGCTTGTCATCTTGATTGCTTTGCTTGGGTCAAACGTGATAGTTATCTGCCCCAAGGGAGCCAGGGCCTTAAGGCTGTTACAAAGGCAAAGTTGGGTTATGATCCAATAGAGGTGAATCCTGAGGATATGGTTCGCTTTGCGAAAGAAGAACCTCAGAGGATGGCATCCTATTCTGTTTCTGATGCTGTTGCAACCTATTACTTGTACATGACCTATGTTCATCCATTTATCTTCTCTCTTGCAACTATTATTCCAATGTCTCCGGATGAGGTTTTGCGTAAGGGTAGTGGAACCCTTTGTGAAATGCTTTTAATGGTCCAGGCATACAAGGCAAATGTCATCTGTCCTAACAAGCACCAATCTGACCCGGAAAAGTTCTATAAAAACCACCTTTTGGAAAGTGAGACTTACATAGGTGGCCATGTGGAATGCCTTGAAAGTGGTGTTTTCAGATCTGATCTTCCAACTAATTTTAGGCTTGATCCAGCTGCTTATGAGCAATTGATCAACAACCTTGATCGAGATCTTCAATATGCTATCAGAGTAGAGGGAAAGATGGACTTGGATTCTGTGTCCAATTATGATGAAGTGAAGGGTTCTATTCTGGAAAAGCTTGTGAGGTTGCGAGATGAACCAATACGAGAAGAATGCCCACTTATTTATCACCTGGATGTAGCTGCAATGTATCCCAACATTATTTTAACAAACAGGCTTCAGCCCACATCAATTGTTACAGATGAGGTCTGCACTGCTTGTGATTTCAACCGTCCAGGGAAGACATGTCTACGAAAGCTGGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAAAAGAAGCGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTCTGTTGACGGTGCCACTTCCAAGCAGTCAAGGTCTTTTCTTGACTTGCCTAAAATGGAACAACAAACAAGGCTCAAAGAACGTCTAAAGAAATACTGTCAGAAGGCATATAAACGTGTAGTAGACAAGCCAGTCACTGAACTTCGGGAAGCAGAGATTTGCATGAGGGAAAATTCATTCTATATTGACACAGTTCGCAGCTTTCGAGATAGAAGGTACGAGTATAAAGGGCTTAATAAGGTTTGGAAGGGCAAGTTGTCTGAAGCAAAGGCTAGTAAAAATTCTATAAAAATCCAGGAAGCACAAAATATGGTGGTGCTTTATGATTCCCTACAACTTGCACATAAGTGTATACTTAACTCCTTTTATGGATACGTCATGCGCAAGGGTGCAAGATGGTATTCAATGGAAATGGCTGGAGTAGTTACATATACTGGAGCTAAAATTATTCAGAATGCTCGGCTGCTAGTTGAAAAAATTGGAAAGCCTCTTGAGTTAGATACAGATGGTATCTGGTGTGTGCTTCCTGGATCTTTTCCAGAGAACTTTACTTTCAAAACAAAAGACTTGAGGAAGAAGCTAACTATCTCATATCCATGCGTCATGCTTAATGTTGATGTGGCGAGAAACAATACAAACGATCAATACCAGACTCTTACAGATCCGATCGAGAAAACTTATGCAACTCATAGTGAATGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTATTCCTGCTTCTAAGGAAGAAGGAATTCTAATTAAGAAGCGCTATGCTGTCTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTTGAGATCAAACGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAACTTTTTGATAAGTTCCTTCACGGTTCTACATTAGAGGAATGCTATTCAGCAGTTGCTTCTGTTGCCAACCGCTGGCTTGATCTTCTTGATAATCAAGGAAAGGATATAGCAGACAGTGAGTTGCTTGATTATATATCAGAATCAAGCACCATGAGCAAGTCTTTGGCGGAATATGGAAGCCAAAAGTCATGTGCAGTAACCACTGCAAGGCGTCTTGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTACGTTGCCAGTATATAGTTGCATGTGAACCAAAGGGAACACCTGTCAGTGAGCGTGCTGTTCCAGTTGCAATATTCGAAACAGATGCTGAAATTATGAAGTTTTATGTACGGAAGTGGTGCAAGGTCTCTGATGTTGACATCAGATCCATTATAGATTGGTCCTATTACAAACAACGTCTAGGTTCAGCAATTCAGAAAATCGTCACAATTCCCGCTGCAATGCAAAAGGTTGCAAATCCTGTCCCTAGGGTAGTCCATCCTGATTGGCTACATAAGAAGGTTCGTGAGAAGGAAGATAAATTTCGACAACGCAAATTGGTTGATATGTTCAGGTCGAATGGGGATCATCTGGTGCAAAACGATGATGCTATTGGCTCTAATCATGTGGTAGATGAAGATATTGTTGAAGATTTGGAAGACTTCAAATGCAAAAGCAGTTCCATAAGTAGACCGAGACCAATTGTTCACTGTTATGAAGTTAATAACCAGAAAAATTCAGTCAAGGCAAACGGTAAAGGAGGTCCAGAGCAACAACAAACTAATCATATAGAAAATGCTCATCAAGAGCAAACTGCTGTCACTCAGGAAGAAATTGACAAAAATGTAGATTATCAAGGATGGCTTGCAATAAAGAAAAGAAAGTGGAAAGATACTCTTGACAAGAGGAAGAAACAACGATTGAATAATGCAAGGTCTGCTCATAATGCTAATGACAAGATGCTTGGTGGTGGGAGAAATCTCAAAGGGGCTCAGGGGAAAATAGGTGTGAATTCATATTTTAGGAGTCATGAAGATGCCCTAACTCGCTGTCATTGGCAGATATTACAACTAGTTCCGAGTTCTGAGAGTGGCCAAATTTTGGCGTGGCTTGTTGTTGAAGGAATGATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATCAATTCTACATCACCTATAAAAGAAGAATTGAAAGAAGAATTACCAGGGAAGTATGTCAACAAGACTCTTCCTCATGGAAGACATAGTTATTACCTATTTGAGGTTTCAGTTGATGAGAAGAGGTTTAGGACACTGAGAAAAAAGCTTGCGGCTCTTATCGCTGATCCAGATATTGAGGGAATATATGAAACAAAGGTGCCTTCGGAGTTTAATGTAATCACTCAGATTGGTTGTGTCTGTAAATTGGACAAAAAAGCTCAGGGAGATGCCAAAAGTGGCTGGAGTGTTAGTGCACTGGACATGAAGAATACGACAGAGTGCTCTTATCTGGCCCAATCCATTGCTTTCTTTTATTTGTATCACAGCATCTCTGAAGGGCGCGCAATATTTGTTGCTTATTTTCCTGCATCAAGAACAATAATTGTTGTGGTGGTGAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAAAAACAATTCCGCGACGCTTGCCAGGCATTATCACTTCAGCTGCCTCCTAGGAATGGAATCATTATCAAGGTAGACTACGTTGGATATGTAAAGGATGCTGAAAAGATTTTGCACCAAGCAATTAATGAGCACAGACATGATCATGGACCTACAGTTGCTGTTATTGAATGCCCAAATGTTCAGTTAATGAAGTCAGGTATACGAGCTCTTGATGATTTTCCATGTGTGAGCATTCCATGTAATGCTCGAGACAGTCAATATCAGGTCCTTGGGTGGCAACAAGTGGCCGCAAAAATTGGCATGCAACGATGTGCTGCATCATCTCAGTGGTTGATTGATAGAATTTCTCTCTCAAGATATGCACATGTACCATTGGGCAATTTTGAAGTTGACTCCCTTATGCATACAGCTGATATCTTCTTTTCAAGAGCATTACGTGATAGCCAGCAGGTGCTTTGGATATCTGATGATGGTATTCCAGATCTAGGAGGCATTAACGAAGAAGGTGGATTTTTTGCAGATGAGGTCCATCAACCATTTGTAATTACTAAACCTGGAGCATATAGAAAGGTCACTGTGGAGCTAAAGATACATCATCTGGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTGAATGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACATGAACTCCGGATCACATACCTCTAATATGCAGGCTGCCTTTGATGAAGCTACATCATCTGCACCTGCATTCCGTGTTTTGAAACAGTTGATCCACCGGTGTCTTGCAGATGCACATACATCTGGAAATGTGTATGCTGATGCACTACTGCAACATCTGTATCGATGGCTTTGCAGCCCCCAGTCTAAACTTCATGATCCAGCACTTCAACGCCTACTTCACAAGGTCATGAAAAAGGTGCTTTTGATTTTATTGGATGAGTTCCGCAAATTGGGTGCAACTATCATATTTGCAAATTTCTATAAGGTTATTATAGACACAGGAAAATTTGATATATCCGCAGCCAAAGCTTATTGTGATAGTGTGCTTAAAACTCTCCAGAAAAGAGAACTATTTGAGTGGATTGAGCTTGAACCATTGCACTACTGGCATTCTTTGCTTTTCATGGATCAGTATAATTATGGTGGAATTCCGGTTAGAGCTGATGAAAGCATGCATGGTGAATCTCAAGTGGATATCGTATCGAGCTGGAACATTGCTGAATACTTACCCAAGAAAATTCAGGACCATTTTATTTTTATTGTTTCTCAGTTTTTGTATATTCCCTGGAATTATGCACAGAAGCAGTCGGCCATTAGAGCGACTTTACAGGATGGCAGCAGTTGCACACAATCAATTACGGTTGCAGCTGCTGAGACTTTGGAATCTCACATGACCGATTTTCTTAAAGGACAGATCAGCTCGTACTTTACTGAAAAGCTTCTGGGAATTGTTCGTGATACAATACTTCATATGAAGGAACTGAACAAGTCCGAGAATGACTATCGGACATCTCCTGGGCTGCCACAACTTGCTGGTAATATCCACAAAGGAGATGCGGCACTTGAGTTCATTAAGAATGTCTGCGGTGTTTTGCTCATTGACCAAAGTGTTGAGCACGATGTTATGGTAATGAGAAGTAATCTATTAAAATATATGCATGTGAGGGAGTCTGCTCCTGAAGCTCAGTTTTGTGATCCCTGCCCATCCTTGTTCTTACGAAATGTCATTTGCAGCTACTGCAATGACTGTAGAGACCTAGACATCTGCCGTGATTCAGCTCTACTAGCTGGGGAGTGGCGCTGTGCTGTGCCCCAATGCGGGCAGCCTTATGACAGAGAGCTTATGGAGAATACCCTTCTTCAGATTGTACAGCAGAGAGAGCGTCTCTACCAGCTACAGGATCTGGTGTGTCTCAGGTGTAACCAAGTGAAAGCTGCACATTTAGCAGAACAATGTGGATGTGCCGGCTCTTTTAGCTGTAAGGAAGACGCAGCTGAATTTTGTGAAAAGATGCAGCTAATCTTGAAAATTGCAATTCATCAGAAATTTCAATTTCTACAAGAGTGCACTGAGTGGATTTTGGAAGTTGAGAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2213

Amino Acids

253.41

Weight (kDa)

6.39

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 196 - 402 6.9e-56 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 750 - 1073 2.5e-18 DNA polymerase family B
POL2_thumb PF22634 948 - 1122 2.9e-78 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1531 - 1897 1.1e-113 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2074 - 2140 1.5e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2145 - 2202 5.3e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 4984, 5293
AasI GACNNNNNNGTC 1 cut(s) 2132
Acc16I TGCGCA 1 cut(s) 2384
Acc36I ACCTGC 2 cut(s) 4984, 5293
Acc65I GGTACC 1 cut(s) 834
AccB1I GGYRCC 3 cut(s) 834, 2012, 4264
AccB7I CCANNNNNTGG 3 cut(s) 1522, 2955, 4000
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 6 cut(s) 1912, 2124, 2353, 3589, 4617, 4744
AccII CGCG 4 cut(s) 41, 73, 4449, 4557
AccIII TCCGGA 2 cut(s) 1390, 5228
AcoI YGGCCR 5 cut(s) 649, 1546, 3991, 4830, 5850
AcuI CTGAAG 9 cut(s) 537, 744, 1826, 2283, 2876, 4461, 4565, 6240, 6386
AcyI GRCGYC 1 cut(s) 3094
AdeI CACNNNGTG 1 cut(s) 6615
AfaI GTAC 9 cut(s) 659, 836, 887, 1334, 2219, 3249, 4914, 5966, 6411
AfeI AGCGCT 1 cut(s) 2783
AflII CTTAAG 2 cut(s) 1196, 4031
AflIII ACRYGT 4 cut(s) 39, 1907, 2116, 4909
AgeI ACCGGT 1 cut(s) 5316
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 3970
AleI CACNNNNGTG 1 cut(s) 2347
Alw21I GWGCWC 7 cut(s) 50, 4363, 4391, 4677, 4753, 6159, 6611
Alw44I GTGCAC 2 cut(s) 4359, 6607
AlwNI CAGNNNCTG 7 cut(s) 785, 2138, 3227, 4703, 5912, 6279, 6445
Ama87I CYCGRG 2 cut(s) 92, 4791
Aor13HI TCCGGA 2 cut(s) 1390, 5228
Aor51HI AGCGCT 1 cut(s) 2783
ApaLI GTGCAC 2 cut(s) 4359, 6607
AseI ATTAAT 3 cut(s) 452, 846, 4668
AsiGI ACCGGT 1 cut(s) 5316
Asp700I GAANNNNTTC 2 cut(s) 2766, 5979
Asp718I GGTACC 1 cut(s) 834
AspA2I CCTAGG 2 cut(s) 3386, 4592
AspLEI GCGC 7 cut(s) 75, 333, 2385, 2784, 4449, 4451, 6342
AsuC2I CCSGG 1 cut(s) 1496
AsuII TTCGAA 1 cut(s) 3216
AvaI CYCGRG 2 cut(s) 92, 4791
AvrII CCTAGG 2 cut(s) 3386, 4592
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 3 cut(s) 4363, 6354, 6611
BaeI ACNNNNGTAYC 6 cut(s) 818, 851, 3108, 3141, 5363, 5396
BalI TGGCCA 3 cut(s) 651, 1548, 3993
BanI GGYRCC 3 cut(s) 834, 2012, 4264
BanII GRGCYC 3 cut(s) 50, 3892, 4753
BarI GAAGNNNNNNTAC 2 cut(s) 5142, 5174
BbsI GAAGAC 5 cut(s) 93, 1910, 3564, 4153, 6534
Bbv12I GWGCWC 7 cut(s) 50, 4363, 4391, 4677, 4753, 6159, 6611
BceAI ACGGC 2 cut(s) 344, 6294
BcgI CGANNNNNNTGC 6 cut(s) 1758, 1792, 3206, 3240, 5373, 5407
BciVI GTATCC 3 cut(s) 682, 1824, 2366
BclI TGATCA 2 cut(s) 1632, 4684
BcnI CCSGG 1 cut(s) 1496
BcuI ACTAGT 1 cut(s) 3970
BfmI CTRYAG 5 cut(s) 713, 4695, 5148, 6289, 6437
BfoI RGCGCY 3 cut(s) 334, 2785, 6343
BfrI CTTAAG 2 cut(s) 1196, 4031
BfuAI ACCTGC 2 cut(s) 4984, 5293
BfuI GTATCC 3 cut(s) 682, 1824, 2366
BglII AGATCT 5 cut(s) 376, 706, 1580, 1651, 5026
BlnI CCTAGG 2 cut(s) 3386, 4592
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 2 cut(s) 92, 4791
BmiI GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4266
BmrI ACTGGG 1 cut(s) 5395
BmuI ACTGGG 1 cut(s) 5395
BpiI GAAGAC 5 cut(s) 93, 1910, 3564, 4153, 6534
BplI GAGNNNNNCTC 2 cut(s) 6411, 6443
BpmI CTGGAG 5 cut(s) 2439, 2457, 4369, 5121, 5582
Bpu10I CCTNAGC 2 cut(s) 3891, 4326
Bpu14I TTCGAA 1 cut(s) 3216
BpuEI CTTGAG 7 cut(s) 546, 713, 2513, 2590, 4882, 5712, 6125
BpuMI CCSGG 1 cut(s) 1496
Bsa29I ATCGAT 1 cut(s) 5383
BsaAI YACGTR 1 cut(s) 4982
BsaBI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5212
BsaHI GRCGYC 1 cut(s) 3094
BsaI GGTCTC 3 cut(s) 3271, 3589, 6288
BsaJI CCNNGG 9 cut(s) 1180, 1189, 1898, 3386, 4112, 4592, 4813, 5825, 6056
BsaWI WCCGGW 5 cut(s) 30, 1390, 5228, 5316, 5692
BsaXI ACNNNNNCTCC 4 cut(s) 4342, 4372, 5210, 5240
Bse118I RCCGGY 2 cut(s) 5316, 6506
Bse1I ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 4368, 5401, 5651
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 7 cut(s) 1815, 2742, 3366, 4407, 5636, 5754, 6289
Bse8I GATNNNNATC 4 cut(s) 381, 1170, 1650, 5212
BseAI TCCGGA 2 cut(s) 1390, 5228
BseCI ATCGAT 1 cut(s) 5383
BseDI CCNNGG 9 cut(s) 1180, 1189, 1898, 3386, 4112, 4592, 4813, 5825, 6056
BseJI GATNNNNATC 4 cut(s) 381, 1170, 1650, 5212
BseMI GCAATG 7 cut(s) 1815, 2742, 3366, 4407, 5636, 5754, 6289
BseNI ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 4368, 5401, 5651
BsePI GCGCGC 1 cut(s) 4447
BseRI GAGGAG 1 cut(s) 712
BseSI GKGCMC 3 cut(s) 4363, 6354, 6611
BseYI CCCAGC 1 cut(s) 6329
BsgI GTGCAG 5 cut(s) 1035, 1858, 3096, 5265, 6462
Bsh1236I CGCG 4 cut(s) 41, 73, 4449, 4557
Bsh1285I CGRYCG 1 cut(s) 2670
BshNI GGYRCC 3 cut(s) 834, 2012, 4264
BshTI ACCGGT 1 cut(s) 5316
BshVI ATCGAT 1 cut(s) 5383
BsiEI CGRYCG 1 cut(s) 2670
BsiHKAI GWGCWC 7 cut(s) 50, 4363, 4391, 4677, 4753, 6159, 6611
BsiHKCI CYCGRG 2 cut(s) 92, 4791
BsiSI CCGG 8 cut(s) 19, 31, 1391, 1496, 5229, 5317, 5693, 6507
BslFI GGGAC 3 cut(s) 41, 2821, 3368
BsmBI CGTCTC 3 cut(s) 30, 319, 6431
BsmFI GGGAC 3 cut(s) 41, 2821, 3368
BsmI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4718, 4776, 5288, 5650
Bso31I GGTCTC 3 cut(s) 3271, 3589, 6288
BsoBI CYCGRG 2 cut(s) 92, 4791
Bsp119I TTCGAA 1 cut(s) 3216
Bsp1286I GDGCHC 9 cut(s) 50, 3892, 4363, 4391, 4677, 4753, 6159, 6354, 6611
Bsp13I TCCGGA 2 cut(s) 1390, 5228
Bsp1407I TGTACA 2 cut(s) 1332, 6409
BspDI ATCGAT 1 cut(s) 5383
BspEI TCCGGA 2 cut(s) 1390, 5228
BspFNI CGCG 4 cut(s) 41, 73, 4449, 4557
BspHI TCATGA 3 cut(s) 3928, 5413, 5448
BspLI GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4266
BspMAI CTGCAG 1 cut(s) 717
BspMI ACCTGC 2 cut(s) 4984, 5293
BspQI GCTCTTC 1 cut(s) 5163
BspT104I TTCGAA 1 cut(s) 3216
BspT107I GGYRCC 3 cut(s) 834, 2012, 4264
BspTI CTTAAG 2 cut(s) 1196, 4031
BspTNI GGTCTC 3 cut(s) 3271, 3589, 6288
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 7 cut(s) 1815, 2742, 3366, 4407, 5636, 5754, 6289
BsrFI RCCGGY 2 cut(s) 5316, 6506
BsrGI TGTACA 2 cut(s) 1332, 6409
BsrI ACTGG 9 cut(s) 652, 675, 2132, 2440, 3148, 3203, 4368, 5401, 5651
BssAI RCCGGY 2 cut(s) 5316, 6506
BssECI CCNNGG 9 cut(s) 1180, 1189, 1898, 3386, 4112, 4592, 4813, 5825, 6056
BssHII GCGCGC 1 cut(s) 4447
BssNAI GTATAC 2 cut(s) 2354, 4745
BssNI GRCGYC 1 cut(s) 3094
BssT1I CCWWGG 4 cut(s) 1180, 3386, 4592, 4813
Bst1107I GTATAC 2 cut(s) 2354, 4745
Bst6I CTCTTC 3 cut(s) 4140, 4181, 5163
BstACI GRCGYC 1 cut(s) 3094
BstAFI CTTAAG 2 cut(s) 1196, 4031
BstAPI GCANNNNNTGC 4 cut(s) 3836, 5656, 5883, 6279
BstAUI TGTACA 2 cut(s) 1332, 6409
BstBAI YACGTR 1 cut(s) 4982
BstBI TTCGAA 1 cut(s) 3216
BstENI CCTNNNNNAGG 1 cut(s) 4166
BstFNI CGCG 4 cut(s) 41, 73, 4449, 4557
BstH2I RGCGCY 3 cut(s) 334, 2785, 6343
BstHHI GCGC 7 cut(s) 75, 333, 2385, 2784, 4449, 4451, 6342
BstMCI CGRYCG 1 cut(s) 2670
BstNSI RCATGY 7 cut(s) 722, 1911, 3165, 4849, 4913, 5716, 6203
BstSFI CTRYAG 5 cut(s) 713, 4695, 5148, 6289, 6437
BstSLI GKGCMC 3 cut(s) 4363, 6354, 6611
BstUI CGCG 4 cut(s) 41, 73, 4449, 4557
BstV2I GAAGAC 5 cut(s) 93, 1910, 3564, 4153, 6534
BstX2I RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5026, 6442
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5026, 6442
BstZ17I GTATAC 2 cut(s) 2354, 4745
Bsu15I ATCGAT 1 cut(s) 5383
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 3 cut(s) 682, 1824, 2366
BsuTUI ATCGAT 1 cut(s) 5383
BtgZI GCGATG 1 cut(s) 658
BtsI GCAGTG 2 cut(s) 1875, 3084
BveI ACCTGC 2 cut(s) 4984, 5293
CaiI CAGNNNCTG 7 cut(s) 785, 2138, 3227, 4703, 5912, 6279, 6445
CciI TCATGA 3 cut(s) 3928, 5413, 5448
CfoI GCGC 7 cut(s) 75, 333, 2385, 2784, 4449, 4451, 6342
Cfr10I RCCGGY 2 cut(s) 5316, 6506
ClaI ATCGAT 1 cut(s) 5383
CseI GACGC 7 cut(s) 47, 196, 2590, 3083, 4568, 6413, 6539
Csp6I GTAC 9 cut(s) 658, 835, 886, 1333, 2218, 3248, 4913, 5965, 6410
CspAI ACCGGT 1 cut(s) 5316
CspCI CAANNNNNGTGG 2 cut(s) 1837, 1872
CviQI GTAC 9 cut(s) 658, 835, 886, 1333, 2218, 3248, 4913, 5965, 6410
DraIII CACNNNGTG 1 cut(s) 6615
DrdI GACNNNNNNGTC 1 cut(s) 2132
DriI GACNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 2132
EaeI YGGCCR 5 cut(s) 649, 1546, 3991, 4830, 5850
Eam1104I CTCTTC 3 cut(s) 4140, 4181, 5163
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 3 cut(s) 4140, 4181, 5163
EciI GGCGGA 1 cut(s) 3065
Ecl136II GAGCTC 2 cut(s) 48, 4751
Eco130I CCWWGG 4 cut(s) 1180, 3386, 4592, 4813
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 3 cut(s) 50, 3892, 4753
Eco31I GGTCTC 3 cut(s) 3271, 3589, 6288
Eco32I GATATC 4 cut(s) 355, 4960, 5007, 5737
Eco47III AGCGCT 1 cut(s) 2783
Eco53kI GAGCTC 2 cut(s) 48, 4751
Eco57I CTGAAG 9 cut(s) 537, 744, 1826, 2283, 2876, 4461, 4565, 6240, 6386
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 2 cut(s) 92, 4791
EcoICRI GAGCTC 2 cut(s) 48, 4751
EcoNI CCTNNNNNAGG 1 cut(s) 4166
EcoO109I RGGNCCY 3 cut(s) 1192, 2808, 4810
EcoRI GAATTC 3 cut(s) 2766, 3910, 5688
EcoRV GATATC 4 cut(s) 355, 4960, 5007, 5737
EcoT14I CCWWGG 4 cut(s) 1180, 3386, 4592, 4813
EcoT22I ATGCAT 3 cut(s) 4950, 5718, 6201
EcoT38I GRGCYC 3 cut(s) 50, 3892, 4753
ErhI CCWWGG 4 cut(s) 1180, 3386, 4592, 4813
Esp3I CGTCTC 3 cut(s) 30, 319, 6431
FaqI GGGAC 3 cut(s) 41, 2821, 3368
FauI CCCGC 4 cut(s) 673, 969, 3361, 6353
FauNDI CATATG 2 cut(s) 972, 6012
FbaI TGATCA 2 cut(s) 1632, 4684
FblI GTMKAC 6 cut(s) 1912, 2124, 2353, 3589, 4617, 4744
FriOI GRGCYC 3 cut(s) 50, 3892, 4753
FspI TGCGCA 1 cut(s) 2384
GlaI GCGC 7 cut(s) 74, 332, 2384, 2783, 4448, 4450, 6341
GsaI CCCAGC 1 cut(s) 6333
GsuI CTGGAG 5 cut(s) 2439, 2457, 4369, 5121, 5582
HaeII RGCGCY 3 cut(s) 334, 2785, 6343
HapII CCGG 8 cut(s) 19, 31, 1391, 1496, 5229, 5317, 5693, 6507
HgaI GACGC 7 cut(s) 47, 196, 2590, 3083, 4568, 6413, 6539
HhaI GCGC 7 cut(s) 75, 333, 2385, 2784, 4449, 4451, 6342
Hin1I GRCGYC 1 cut(s) 3094
Hin6I GCGC 7 cut(s) 73, 331, 2383, 2782, 4447, 4449, 6340
HinP1I GCGC 7 cut(s) 73, 331, 2383, 2782, 4447, 4449, 6340
HincII GTYRAC 5 cut(s) 823, 2008, 3277, 4126, 4936
HindII GTYRAC 5 cut(s) 823, 2008, 3277, 4126, 4936
HindIII AAGCTT 5 cut(s) 519, 1739, 4211, 5568, 5978
HpaII CCGG 8 cut(s) 19, 31, 1391, 1496, 5229, 5317, 5693, 6507
Hpy99I CGWCG 4 cut(s) 86, 212, 488, 4562
Hsp92I GRCGYC 1 cut(s) 3094
HspAI GCGC 7 cut(s) 73, 331, 2383, 2782, 4447, 4449, 6340
KflI GGGWCCC 1 cut(s) 2808
Kpn2I TCCGGA 2 cut(s) 1390, 5228
KpnI GGTACC 1 cut(s) 838
KroI GCCGGC 1 cut(s) 6506
KroNI GCCGGC 1 cut(s) 6508
Ksp22I TGATCA 2 cut(s) 1632, 4684
LguI GCTCTTC 1 cut(s) 5163
LmnI GCTCC 8 cut(s) 45, 53, 863, 1185, 2438, 5102, 5125, 6220
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 4 cut(s) 1628, 1854, 2703, 3600
MflI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5026, 6442
MhlI GDGCHC 9 cut(s) 50, 3892, 4363, 4391, 4677, 4753, 6159, 6354, 6611
MlsI TGGCCA 3 cut(s) 651, 1548, 3993
MluI ACGCGT 1 cut(s) 39
MluNI TGGCCA 3 cut(s) 651, 1548, 3993
MlyI GAGTC 9 cut(s) 104, 536, 2003, 2009, 2647, 3934, 4126, 4931, 6218
MmeI TCCRAC 4 cut(s) 841, 1063, 1616, 4606
Mox20I TGGCCA 3 cut(s) 651, 1548, 3993
Mph1103I ATGCAT 3 cut(s) 4950, 5718, 6201
MroI TCCGGA 2 cut(s) 1390, 5228
MroNI GCCGGC 1 cut(s) 6506
MroXI GAANNNNTTC 2 cut(s) 2766, 5979
MscI TGGCCA 3 cut(s) 651, 1548, 3993
MslI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3612, 3933, 4680, 4686, 4870
Msp20I TGGCCA 3 cut(s) 651, 1548, 3993
MspA1I CMGCKG 7 cut(s) 1616, 2955, 3356, 4585, 4955, 5909, 6536
MspCI CTTAAG 2 cut(s) 1196, 4031
MspI CCGG 8 cut(s) 19, 31, 1391, 1496, 5229, 5317, 5693, 6507
MunI CAATTG 4 cut(s) 1628, 1854, 2703, 3600
Mva1269I GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4718, 4776, 5288, 5650
MvnI CGCG 4 cut(s) 41, 73, 4449, 4557
NaeI GCCGGC 1 cut(s) 6508
NciI CCSGG 1 cut(s) 1496
NdeI CATATG 2 cut(s) 972, 6012
NgoMIV GCCGGC 1 cut(s) 6506
NlaIV GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4266
NmeAIII GCCGAG 2 cut(s) 120, 2440
NmuCI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3718, 4527, 5116
NsbI TGCGCA 1 cut(s) 2384
NsiI ATGCAT 3 cut(s) 4950, 5718, 6201
NspI RCATGY 7 cut(s) 722, 1911, 3165, 4849, 4913, 5716, 6203
NspV TTCGAA 1 cut(s) 3216
OliI CACNNNNGTG 1 cut(s) 2347
PaeI GCATGC 3 cut(s) 722, 4849, 5716
PaeR7I CTCGAG 2 cut(s) 92, 4791
PagI TCATGA 3 cut(s) 3928, 5413, 5448
PaqCI CACCTGC 2 cut(s) 4984, 5293
PauI GCGCGC 1 cut(s) 4447
PceI AGGCCT 1 cut(s) 559
PciI ACATGT 2 cut(s) 1907, 4909
PciSI GCTCTTC 1 cut(s) 5163
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 2203
PctI GAATGC 8 cut(s) 1562, 1781, 2461, 2927, 4718, 4776, 5288, 5650
PdiI GCCGGC 1 cut(s) 6508
PdmI GAANNNNTTC 2 cut(s) 2766, 5979
PflMI CCANNNNNTGG 3 cut(s) 1522, 2955, 4000
PfoI TCCNGGA 3 cut(s) 1035, 2295, 2527
PinAI ACCGGT 1 cut(s) 5316
Ple19I CGATCG 1 cut(s) 2670
PleI GAGTC 9 cut(s) 103, 535, 2002, 2008, 2647, 3933, 4126, 4931, 6217
PpsI GAGTC 9 cut(s) 103, 535, 2002, 2008, 2647, 3933, 4126, 4931, 6217
Ppu21I YACGTR 1 cut(s) 4982
PpuMI RGGWCCY 2 cut(s) 2808, 4810
PscI ACATGT 2 cut(s) 1907, 4909
PshBI ATTAAT 3 cut(s) 452, 846, 4668
Psp124BI GAGCTC 2 cut(s) 50, 4753
Psp5II RGGWCCY 2 cut(s) 2808, 4810
PspFI CCCAGC 1 cut(s) 6329
PspN4I GGNNCC 8 cut(s) 29, 836, 1187, 1420, 2014, 2809, 2810, 4266
PspPPI RGGWCCY 2 cut(s) 2808, 4810
PspXI VCTCGAGB 1 cut(s) 92
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 7 cut(s) 785, 2138, 3227, 4703, 5912, 6279, 6445
PsuI RGATCY 9 cut(s) 376, 706, 1580, 1651, 2531, 2662, 3284, 5026, 6442
PteI GCGCGC 1 cut(s) 4447
PvuI CGATCG 1 cut(s) 2670
PvuII CAGCTG 5 cut(s) 1616, 4585, 4955, 5909, 6536
RsaI GTAC 9 cut(s) 659, 836, 887, 1334, 2219, 3249, 4914, 5966, 6411
RsaNI GTAC 9 cut(s) 658, 835, 886, 1333, 2218, 3248, 4913, 5965, 6410
RseI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3612, 3933, 4680, 4686, 4870
SacI GAGCTC 2 cut(s) 50, 4753
SapI GCTCTTC 1 cut(s) 5163
SchI GAGTC 9 cut(s) 104, 536, 2003, 2009, 2647, 3934, 4126, 4931, 6218
SduI GDGCHC 9 cut(s) 50, 3892, 4363, 4391, 4677, 4753, 6159, 6354, 6611
SfcI CTRYAG 5 cut(s) 713, 4695, 5148, 6289, 6437
Sfr274I CTCGAG 2 cut(s) 92, 4791
SfuI TTCGAA 1 cut(s) 3216
SgrAI CRCCGGYG 1 cut(s) 5316
SlaI CTCGAG 2 cut(s) 92, 4791
SmiMI CAYNNNNRTG 9 cut(s) 735, 1797, 2347, 2596, 3612, 3933, 4680, 4686, 4870
SpeI ACTAGT 1 cut(s) 3970
SphI GCATGC 3 cut(s) 722, 4849, 5716
SseBI AGGCCT 1 cut(s) 559
SspI AATATT 3 cut(s) 1947, 3213, 4455
SstI GAGCTC 2 cut(s) 50, 4753
StuI AGGCCT 1 cut(s) 559
StyI CCWWGG 4 cut(s) 1180, 3386, 4592, 4813
TaqII GACCGA 2 cut(s) 3607, 5951
TatI WGTACW 3 cut(s) 885, 1332, 6409
TauI GCSGC 4 cut(s) 9, 4220, 4835, 6101
TseFI GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3718, 4527, 5116
Tsp45I GTSAC 9 cut(s) 467, 922, 1000, 1094, 2134, 3343, 3718, 4527, 5116
TspGWI ACGGA 3 cut(s) 840, 3265, 5282
Van91I CCANNNNNTGG 3 cut(s) 1522, 2955, 4000
Vha464I CTTAAG 2 cut(s) 1196, 4031
VneI GTGCAC 2 cut(s) 4359, 6607
VspI ATTAAT 3 cut(s) 452, 846, 4668
XagI CCTNNNNNAGG 1 cut(s) 4166
XceI RCATGY 7 cut(s) 722, 1911, 3165, 4849, 4913, 5716, 6203
XcmI CCANNNNNNNNNTGG 1 cut(s) 5643
XhoI CTCGAG 2 cut(s) 92, 4791
XmaJI CCTAGG 2 cut(s) 3386, 4592
XmiI GTMKAC 6 cut(s) 1912, 2124, 2353, 3589, 4617, 4744
XmnI GAANNNNTTC 2 cut(s) 2766, 5979
Zsp2I ATGCAT 3 cut(s) 4950, 5718, 6201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.