Rroxscaffold_1G00026140

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001971
Physical Location & Seq
Reverse (-)
32853032 .. 32855159
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026140.1

Sequence Viewer

Length: 780 bp
ATGTCAGCATCTCTGAAGGGCGCGCAATATTTGTTGCTTAATTATTTTCCTGCATCAAGAACAATAATTGTTGTGGTGGTGAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAAAAACAATTCCGCGACGCTTGCCAGGCATTATCACTTCAACTGCCTCCTAGGAATGGAATCATTATCAAGGTAGACTACGTTGGATATGTAAAGGATGCTGAAAAGATTTTGCACCAAGCAATTAATGAGCACAGACATGATCATGGACCTCCAATTGCTGTTATTGAATGCCCAAATGTTCAGTTAATGAAGTCAGGTATACGAGCTCTTGATGATTTTCCATGTGTGAGCATTCCGTGTAATGCTCGAGACAGTCAATATCAGGTCCTTGGGTGGCAACAAGTGGCTGCAAAAATTGGCATGCAACGATGTGCTGCATCATCTCAGTGGTTGATTGATAGAATTTCTCTCTCAAGATATGCACATGTACCATTGGGCAATTTTGAAGTTGACTCCCTTATGTATACAGCTGATATGTTCTTTTCAAGAGCATTACGTGATATCTTGCACAATATTTTTATGTTTTCAGAAATAAATGGTTTTGTCGGACTGAAGCTTTTTAAGGTACTTTGGATATCTGATATTGGTACTCCAGATCTAGGAGGCATTAACGAAGAAGGTGGATTTTTTGCAGATGAGGTTAGTCTCAAACTGACATATGGATGTCGGGATGTCACGCCCCTAATTTTGAACAACAACTTCAAATTCGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

259

Amino Acids

29.13

Weight (kDa)

6.3

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1744 PF08490 57 - 188 2.1e-28 Domain of unknown function (DUF1744)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 195, 322, 527
AccII CGCG 2 cut(s) 23, 135
AciI CCGC 1 cut(s) 133
AcsI RAATTY 2 cut(s) 465, 767
AcuI CTGAAG 2 cut(s) 35, 635
AfaI GTAC 3 cut(s) 492, 630, 652
AfiI CCNNNNNNNGG 2 cut(s) 176, 662
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 7 cut(s) 122, 161, 290, 509, 549, 754, 766
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 329, 533, 619
AluI AGCT 3 cut(s) 329, 533, 619
Alw21I GWGCWC 2 cut(s) 255, 331
Alw26I GTCTC 2 cut(s) 366, 713
Ama87I CYCGRG 1 cut(s) 369
ApeKI GCWGC 2 cut(s) 410, 437
ApoI RAATTY 2 cut(s) 465, 767
AseI ATTAAT 1 cut(s) 246
AspA2I CCTAGG 1 cut(s) 170
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 2 cut(s) 269, 388
AsuHPI GGTGA 2 cut(s) 91, 99
AsuII TTCGAA 1 cut(s) 771
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 2 cut(s) 269, 388
AvrII CCTAGG 1 cut(s) 170
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 255, 331
BbvI GCAGC 2 cut(s) 397, 424
BccI CCATC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 146
BclI TGATCA 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 366, 713
BfaI CTAG 2 cut(s) 171, 662
BglII AGATCT 1 cut(s) 658
BisI GCNGC 2 cut(s) 411, 438
BlnI CCTAGG 1 cut(s) 170
BlsI GCNGC 2 cut(s) 412, 439
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 2 cut(s) 269, 388
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 269, 388
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 4 cut(s) 17, 62, 208, 449
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 170, 391
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 662
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 170, 391
BseGI GGATG 3 cut(s) 223, 731, 739
BseLI CCNNNNNNNGG 2 cut(s) 176, 662
BseMII CTCAG 1 cut(s) 461
BsePI GCGCGC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 397, 424
Bsh1236I CGCG 2 cut(s) 23, 135
BsiHKAI GWGCWC 2 cut(s) 255, 331
BsiHKCI CYCGRG 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 176, 662
BsmAI GTCTC 2 cut(s) 366, 713
BsmI GAATGC 2 cut(s) 296, 354
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 771
Bsp1286I GDGCHC 2 cut(s) 255, 331
Bsp143I GATC 2 cut(s) 262, 658
BspACI CCGC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 2 cut(s) 23, 135
BspT104I TTCGAA 1 cut(s) 771
BssECI CCNNGG 2 cut(s) 170, 391
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 2 cut(s) 262, 658
BssNAI GTATAC 2 cut(s) 323, 528
BssT1I CCWWGG 2 cut(s) 170, 391
Bst1107I GTATAC 2 cut(s) 323, 528
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 771
BstC8I GCNNGC 3 cut(s) 23, 142, 425
BstDEI CTNAG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 223, 731, 739
BstFNI CGCG 2 cut(s) 23, 135
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 2 cut(s) 265, 661
BstMAI GTCTC 2 cut(s) 366, 713
BstMBI GATC 2 cut(s) 262, 658
BstMWI GCNNNNNNNGC 2 cut(s) 141, 146
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 2 cut(s) 427, 491
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 2 cut(s) 23, 135
BstV1I GCAGC 2 cut(s) 397, 424
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZ17I GTATAC 2 cut(s) 323, 528
BtsCI GGATG 3 cut(s) 223, 731, 739
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 23, 142, 425
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 2 cut(s) 269, 388
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 491, 629, 651
CviAII CATG 6 cut(s) 260, 266, 345, 424, 488, 777
CviJI RGCY 4 cut(s) 329, 410, 533, 619
CviKI_1 RGCY 4 cut(s) 329, 410, 533, 619
CviQI GTAC 3 cut(s) 491, 629, 651
DdeI CTNAG 1 cut(s) 447
DpnI GATC 2 cut(s) 264, 660
DpnII GATC 2 cut(s) 262, 658
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 170, 391
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 2 cut(s) 565, 639
Eco47I GGWCC 2 cut(s) 269, 388
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 2 cut(s) 35, 635
Eco88I CYCGRG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 144
EcoRV GATATC 2 cut(s) 565, 639
EcoT14I CCWWGG 2 cut(s) 170, 391
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 170, 391
FaeI CATG 6 cut(s) 263, 269, 348, 427, 491, 780
FatI CATG 6 cut(s) 259, 265, 344, 423, 487, 776
FauNDI CATATG 1 cut(s) 721
FbaI TGATCA 1 cut(s) 262
FblI GTMKAC 3 cut(s) 195, 322, 527
Fnu4HI GCNGC 2 cut(s) 411, 438
FokI GGATG 3 cut(s) 230, 738, 746
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 2 cut(s) 411, 438
FspBI CTAG 2 cut(s) 171, 662
GlaI GCGC 2 cut(s) 22, 24
GluI GCNGC 2 cut(s) 411, 438
GsuI CTGGAG 1 cut(s) 639
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 23, 25
Hin1II CATG 6 cut(s) 263, 269, 348, 427, 491, 780
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 3 cut(s) 82, 180, 515
HphI GGTGA 2 cut(s) 91, 99
Hpy166II GTNNAC 4 cut(s) 196, 323, 514, 528
Hpy188I TCNGA 4 cut(s) 15, 592, 611, 643
Hpy188III TCNNGA 8 cut(s) 57, 119, 332, 371, 477, 549, 656, 731
Hpy8I GTNNAC 4 cut(s) 196, 323, 514, 528
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 10, 674
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 2 cut(s) 201, 559
HpyCH4V TGCA 8 cut(s) 53, 235, 413, 427, 440, 485, 571, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 146
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 2 cut(s) 201, 559
Hsp92II CATG 6 cut(s) 263, 269, 348, 427, 491, 780
HspAI GCGC 2 cut(s) 21, 23
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 2 cut(s) 262, 658
LpnPI CCDG 6 cut(s) 63, 131, 158, 303, 371, 669
Lsp1109I GCAGC 2 cut(s) 397, 424
LweI GCATC 4 cut(s) 17, 62, 208, 449
MaeI CTAG 2 cut(s) 171, 662
MaeII ACGT 2 cut(s) 201, 559
MaeIII GTNAC 2 cut(s) 105, 736
MalI GATC 2 cut(s) 264, 660
MboI GATC 2 cut(s) 262, 658
MboII GAAGA 1 cut(s) 689
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 2 cut(s) 255, 331
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 184, 589
MnlI CCTC 4 cut(s) 177, 282, 659, 694
MseI TTAA 5 cut(s) 39, 246, 308, 624, 672
MslI CAYNNNNRTG 4 cut(s) 258, 264, 448, 724
MspA1I CMGCKG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 276
Mva1269I GAATGC 2 cut(s) 296, 354
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 2 cut(s) 23, 135
MwoI GCNNNNNNNGC 2 cut(s) 141, 146
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 262, 658
NlaIII CATG 6 cut(s) 263, 269, 348, 427, 491, 780
NmuCI GTSAC 2 cut(s) 105, 736
NspI RCATGY 2 cut(s) 427, 491
NspV TTCGAA 1 cut(s) 771
PaeI GCATGC 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 369
PauI GCGCGC 1 cut(s) 21
PciI ACATGT 1 cut(s) 487
PctI GAATGC 2 cut(s) 296, 354
PfeI GAWTC 2 cut(s) 82, 180
PkrI GCNGC 2 cut(s) 412, 439
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 388
PscI ACATGT 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspPI GGNCC 2 cut(s) 269, 388
PspPPI RGGWCCY 1 cut(s) 388
PsuI RGATCY 1 cut(s) 658
PteI GCGCGC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 492, 630, 652
RsaNI GTAC 3 cut(s) 491, 629, 651
RseI CAYNNNNRTG 4 cut(s) 258, 264, 448, 724
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 5 cut(s) 39, 246, 308, 624, 672
SatI GCNGC 2 cut(s) 411, 438
Sau3AI GATC 2 cut(s) 262, 658
Sau96I GGNCC 2 cut(s) 269, 388
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 255, 331
SfaNI GCATC 4 cut(s) 17, 62, 208, 449
Sfr274I CTCGAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 771
SinI GGWCC 2 cut(s) 269, 388
SlaI CTCGAG 1 cut(s) 369
SmiMI CAYNNNNRTG 4 cut(s) 258, 264, 448, 724
SmlI CTYRAG 2 cut(s) 369, 475
SmoI CTYRAG 2 cut(s) 369, 475
SphI GCATGC 1 cut(s) 427
SsiI CCGC 1 cut(s) 133
SspI AATATT 2 cut(s) 29, 577
SspMI CTAG 2 cut(s) 171, 662
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 2 cut(s) 170, 391
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 2 cut(s) 204, 562
TaqI TCGA 2 cut(s) 370, 771
TfiI GAWTC 2 cut(s) 82, 180
Tru1I TTAA 5 cut(s) 39, 246, 308, 624, 672
Tru9I TTAA 5 cut(s) 39, 246, 308, 624, 672
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 2 cut(s) 105, 736
TseI GCWGC 2 cut(s) 410, 437
Tsp45I GTSAC 2 cut(s) 105, 736
TspDTI ATGAA 1 cut(s) 326
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 269, 388
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 2 cut(s) 465, 767
XceI RCATGY 2 cut(s) 427, 491
XhoI CTCGAG 1 cut(s) 369
XmaJI CCTAGG 1 cut(s) 170
XmiI GTMKAC 3 cut(s) 195, 322, 527
XspI CTAG 2 cut(s) 171, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.