FvH4_3g29570

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001971
Physical Location & Seq
Forward (+)
22583978 .. 22607046
23069 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29570.t5

Sequence Viewer

Length: 6789 bp
ATGGATACCCAAGCGCGGGAAAATGCTTCCTATCCCTCTCTTATTAGGTCCAGACTGGTCTCAGTGTGCTGCCGCTTTTCAGATCGCGAAGCCCAAATTCGCAGAGAGATAGTTCCCACTGAAAACCCTAAAACCATGAGCGGCTACGAGAGCCGCAAACGCGACCGGAGAGACGCGTGGAGCTCTAAGAAGCAGCAGAAGCTCGCGCCCACCGCCGACGAAGACCTCGAGTCGAAGCTCGGTTTCGATCTGTTCTCCGAAGGCGAAACTCGGCTCGGGTGGTTACTCACTTTCGCTTCTTCATCTGTGGAAGATCAAGACACCCGTAAGGTGTATAGCTGCGTTGATCTCTATTTCGTTACACAGGATGGCTCCGCTTTCAAGTCAAAATATAAGTTTCGGCCGTATTTCTATGCAGCCACAAAGGAGAAAATGGAAATGGATGTTGAAGCATATTTGAAACGGCGTTATGAAAGTCGTATTGCTGATATCGAGATAGTGGGGAAAGAAGACCTTGATCTAAAAAACCATTTGTCTGGCCTACGCAAGTCCTATTTGAAGTTATCATTTGATACTGTTCAACAATTGATGAATGTCAAGAGTGACCTATTGCAAGTTGTTGAAAGAAACCGCACAAAATTTGATGCTGCCGAAGCTTATGAGTCAATACTTCTAGGAAATCGAGAACAAAGGCCACAAGATTTCCTAGATTGTATTATTGATCTTCGTGAATATGATGTTCCCTACCATGTCCGCTTCGCTATTGACAATGATGTGCGATGTGGCCAGTGGTACAATGTTACTGTATCCAGTACAGGTGTTATGCTTGAGAGGAGGACAGATCTTCTGCAGCATGCCGAAGTTCATGTTTGTGCATTCGATATTGAGACAACTAAGCTACCTTTAAAATTTCCAGATGCTGAATATGATTTAGTAATGATGATTTCATACATGGTTGATGGACACGGGTACTTGATTATTAATAGAGAGTGTGTTGGAGCTGATATAGAATATCTGGAGTACACTCCAAAAGAAGAATTTGTAGGCCATTTCAAGGTGACAAATGTGGAAAATGAGAGAGAGCTGTTAAGACACTGGTTTGCCCATATGCGAGAGGTGAAACCTGGAATTTATGTCACATACAATGGTGATTTTTTCGATTGGCCTTTCCTGGAAACCAGAGCTGCACATCATGGGTTCAAAATGAATGAAGAAGTTGGATTTTTATGTGACAAGAATCAGGGGGAATGTAGATCTAAATTTGCTTGTCATCTTGATTGCTTTGCTTGGGTCAAACGTGATAGTTATCTACCCCAAGGGAGCCAGGGCCTTAAGGCTGTTACGAAAGCAAAGTTGGGATATGATCCAATAGAGGTGAATCCTGAGGATATGGTTCGCTTTGCAAAAGAAGAACCTCAGAGGATGGCATCCTATTCGGTTTCTGATGCTGTTGCAACCTATTATTTGTACATGACTTATGTTCATCCATTTATATTCTCTCTTGCAACTATTATTCCAATGTCTCCGGATGAGGTTTTGCGCAAAGGTAGTGGAACCCTTTGTGAAATGCTTTTAATGGTTCAGGCATACAAGGCAAATGTCATATGTCCTAACAAACACCAATCTGACCCGGAAAAGTTCTATAAAAATCACCTTTTGGAAAGTGAGACTTACATAGGTGGCCATGTCGAATGCCTTGAAAGTGGTGTTTTCAGATCTGATCTTCCAACCAGTTTTAGGCTTGATGCAGCTGCTTATGAGCAATTGATCTATAACCTTGATCGAGATCTTCAATATGCTATCAGAGTAGAGGGAAAGATGGACTTGGAATCTGTGTCCAATTATGATGAAGTGAAGAGTTCTATTCTGGAAAAGCTTGTGAGGTTGCGAGATGAACCAATACGAGAAGAATGCCCACTTATTTATCACCTGGATGTAGCTGCTATGTATCCCAACATTATTCTAACAAACAGGCTTCAGCCCCCATCAATAGTTACAGATGAGGTCTGTACTGCTTGTGATTTCAACCGTCCAGGAAAAACATGTCTACGAAAGCTGGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAAAAGAAGCGACTATTATCATCTGAAGAAGCAAATTGAGTCTGAGTCTGTCGACGGTGCCACTTCCAAACAGTCACGGTCTTTTCTTGAATTGCCTAAAATGGAACAACAAACAAGGCTCAAAGAGCGTCTAAAGAAATACTGTCAGAAGGCGTATAAACGAGTAGTGGACAAGCCAGTCACTGAACTCCGGGAAGCAGGGATTTGCATGCGGGAAAATTCATTCTATGTTGACACAGTCCGCAGCTTTCGAGATAGAAGGTATGAGTATAAAGGGCTTAATAAGGTTTGGAAGGGCAAGTTGTCTGAAGCAAAGGCTAGTAAAAATTCTATAAAGATCCAGGAAGCACAAAATATGGTGGTGCTTTATGATTCCTTACAACTTGCACATAAGTGTATACTTAACTCCTTTTATGGATACGTCATGCGCAAGGGTGCAAGGTGGTATTCAATGGAAATGGCTGGAGTAGTTACATATACTGGAGCTAAAATTATTCAGAATGCTCGGTTACTTGTTGAAAAAATTGGAAAACCTCTTGAGTTAGATACAGATGGTATCTGGTGTGTGCTTCCTGGATCATTTCCAGAGAACTTTACGCTCAAAACAAAAGACTTGAAGAAGAAGCTAACTATCTCATATCCATGTGTCATGCTTAATGTTGATGTGGCGAGAAACAATACAAACGATCAGTACCAGACTCTTACAGATCCGATTGAGAAAACTTATGCAACTCATAGTGAATGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTATTCCTGCTTCTAAGGAAGAAGGAATTCTAATCAAGAAGCGCTATGCTGTCTTTAATGACGATGGGACACTTGCTGAACTTAAAGGTTTTGAGATCAAACGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAGCTTTTCGATAAGTTCCTTCACGGTTCTACATTAGAGGAATGTTATTCAGCCGTTGCTTCTGTTGCCAACCGCTGGCTTGATCTTCTTGATAATCAAGGAAAGGATATTGCAGACAGTGAGTTGCTTGATTACATATCAGAATCAAGCACCATGAGCAAGTCTTTGGTGGAATATGGAAGCCAAAAATCGTGTGCGGTAACCACTGCACGGCGTCTTGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTGCGTTGTCAGTATATAGTTGCATGTGAACCACAGGGAGCACCTGTCAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTACGTACGAAAGTGGTGCAAGGTCTCGGATATTGACATCAGATCCATTATAGACTGGTCCTATTACAAACAACGTCTAGGTTCAGCAATTCAGAAAATCGTCACCATTCCCGCTGCAATGCAAAAGGTTGCAAATCCTGTCCCTAGGGTGGTTCATCCTGATTGGCTACATAAGAAGGTTCGTGAGAAGGAAGATAAATTTCGACAACGAAAATTGGTTGATATGTTCACGTCAGTCAACAAGGATCAGTTGGTGGAAAATAATGATGCTATTGGCCCTAATCACGTGGTAGATGAAGATATGGTTGATGATTTAGAAGACTTCAAAAGCAAAAACAGTACCATAAGCAGACCAAGACCGATTGTTCACTGTTATGAAGTCAATAACCAGAAAAAATCAGCCAAGGCAAATGGTGAAAGACATCAACATCAACAACAAACAAATCAAATAGAAATTGATCATCAAGAGCAAATTGCTGTCACTTGGGAAGAGATTGACAAAAGTGTCGATTATCAAGGCTGGCTTGCAATGAAGAAAAGAAAATGGAAAGATACTCTTGACAAGAGGAAGAAACAACGATTGAATAATGCAAGGTCTGCTCATCATGCTCATGACAAGCTCCTTGGTGGTGGGAGACTCAAAGGAGCTCAGGGGAAAATAGGTGTGAATTCATATTTTAGGAGTCATGAGGAAGCCCTAACTCGCTGCCATTGGCAGATATTACAACTAGTTCCGAGTTCTGAGACTGGCCAAATTTTGGCTTGGGTTGTTGTTGAAGGAATGATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATCAATTCTACATCACCTATAAAAGAAGAACTGAAAGAAGAATTACCAGGGAAGTATGTCAACAAGACTCTTCCACATGGAAGACATAGCTATTACCTATATGAGGTTTCAGTTGATGAGAAGAGGAATAGGACAGTGAGGAAAAAGCTTGCGTCTCTTATCGCTGATCCAGATATTGAGGGAATATACGAGACAAAGGTGCCTTCAGAGTTTAATGTAATCACACAGATTGGTTGTGTCTGTAAACTAGACAAAAAAGCTCAGGGAGATGCCAAAAATGGCTGGAGCATTAGTGCACTGGACATGAAGAATACAACAGAGTGCTCTTATCTGGCCCAATCCATCGCTTTCTTTTACTTGTATCACAGCATCGCTGAAGGATCTGAAGGGCGTGCAATATTTGTTGCTTATTTTCCTGCTTCAAGAACAATAACTGTTGTGGTGGTGAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTTGAGAAACAATTCCGGGAAGCTTGTCAGGCATTATCACTTCAGCTGCCTCCTAGGAACGGAATATTTATCAAGGTAGACTACGTTGGATTTGTAAAGGATGCTGAAAAGATTTTGCAGCAAGCAATTAATGAGCACAGACATGAACATCAGGGACCTGCAATTGCTGTTATTGAATGCCCGAATGCTCAATTAATGAAGTCAGGTATACGAGCACTTGATGATTTTCCATGTGTGAGCATTCCGTGTAATGCTCGTGATAGTCAATATCAGGTTCTTGGGTGGCAACAAGTGGCTGCAAAAATTGGCATGCAACGTTGTGCTGCATCATCTCCATGGTTGATTGATAGAATTTCTCTCTCAAGATATTCACACGTTCCATTGGGAAATTTTGAAGTTGACTCCCTTATGCATACAGCTGATATCTTCTTCTCAAGAGCATTACGTGATAGCCAGCAGGTACTTTGGATATCTGATGATGGTATTCCAGATCTAGGAGGCATTAATGAGGAAAGCAGATTTTTTGCAGATGAGGTCCATCAACCATTTGTTATTACTAGACCTGGAGCTTATAGAAAGGTCACTGTGGAGCTAAAGATACATCATCTAGCTGTAGATGCTCTTCTGAAAAGCAACCAAGTAAATGAAATGGAAGGAGGTGCTTTGCTAGGATTTGAAACAGACATGAACACTGGATCACAGACTTTTAATATGCATGCTGCCTTTGATGAAGCTACTTCATCTGCACATGCATTCCGAGTTCTGAAACAGATGATCCTCCGGTGTCTTGCTGATGCAAATACATCTGGAAATGTGTATGCTGATGCATTGCTGCAACATCTGTATCGATGGCTTTGCAGCCCCCAATCTAAACTGCATGATCCAGCACTTCAACGCCTACTTGACAAGGTCATGAAAAAGGTGCTCTTGATTTTATTGGCTGAGTTCCGCAAATTGGGTGCGAATATCATATTTGCGAATTTCTACAAGGTTATTATAGACACAGGAAAATTTGATATATCAGCAGCCAAAGCTTATTGCGATAGCGTGCTTAAAACTATCCAGAAAAGAGAACTATTTGAGTGGATTGAACTTGAACCATTGCACTTCTGGCATTCTTTGCTGTTCATGGATCAGTATAATTATGGTGGAATTCCAGTTAGAGCTAATGATAATGTGTATGGTGAATCTCAAGTGGATATTGTGTCGAGCTGGAACATTGCTGAATACCTGCCCAAGAAAATTCAGGACCATTTTATATTTATTGTTTCCCAGTTTCTATATATTCCCTGGAATTATGCACAGAAGCAGTCTGCCGTTACAGCAACTTTACAGGATGGCAGCAGCTGCACACCATCAATTACGGTTGCAGCTGCTGAGACTTTGGAATCTAACATGACCGATTTTCTCAAAGGACAGATCAGCTCGTACTTTACTGAAAAGCTTCTGGGAATTGTTCGTGATACAATACTTCATATGAAGGAACTGAACAAGTCTGAGAATGACTATCGGACATCTCCTGGCCTGCAACAATTTGTTGGTAATATCCACAAAGGCGATGTGGCGCTTGAGTTCATCAAGAATGTCTGTGGTGTTTTGCTCATTGATCAAAATGTTGAGCATGACGTTATGGTTATGAGAAGTAATTTATTAAAATATATGCATGTGAGGGAGTCTGCTCCTGAAGCTCAGTTTTGTGATCCCTGCCCATCCTTGTTCTTACGAAATGTCATTTGCAGCTACTGCAATGACTGTAGAGACCTGGACATCTGCCGTGATTCAGCTCTACTAGGTGGGGAGTGGCGCTGTGCTGTGCCTCAATGTGGGCAGCCTTATGACAGAGAACTGATGGAGAATACCCTTCTTCAGATTGTACAGCAGAGAGAGCGGCTCTACCACCTACAAGACCTCGTGTGTCTCCGGTGTAACCAAGTGAAAGCTGCACATTTAGCAGAACAATGTGGATGTGCCGGCTCTTTTAGCTGCAAGGAAGACGCAGCTGAATTTTGCGAAAAGATGCAGTTAATCTTGAAAATTTCCATTTATCAGAAATTTCAACTTCTACAAGAGTGCACTGAGTGGATTTTAGAAGTTGTGAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2263

Amino Acids

259.44

Weight (kDa)

6.45

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B PF00136 3 - 303 1.3e-15 DNA polymerase family B
POL2_thumb PF22634 178 - 352 2.4e-78 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 764 - 1131 2.6e-115 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 1308 - 1374 6.2e-20 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 1380 - 1436 7.1e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 2267, 3279, 3881
Acc16I TGCGCA 2 cut(s) 1541, 2519
Acc36I ACCTGC 3 cut(s) 4849, 5131, 5922
AccB1I GGYRCC 2 cut(s) 2147, 4399
AccB7I CCANNNNNTGG 2 cut(s) 3090, 4135
AccBSI CCGCTC 2 cut(s) 141, 6571
AccI GTMKAC 5 cut(s) 2047, 2142, 2488, 4761, 4891
AccII CGCG 5 cut(s) 16, 87, 162, 176, 206
AccIII TCCGGA 1 cut(s) 1525
AclI AACGTT 1 cut(s) 4999
AcoI YGGCCR 4 cut(s) 401, 784, 1681, 4126
AcvI CACGTG 1 cut(s) 3664
AcyI GRCGYC 1 cut(s) 3229
AdeI CACNNNGTG 1 cut(s) 6762
AfeI AGCGCT 1 cut(s) 2918
AflII CTTAAG 2 cut(s) 1331, 4166
AflIII ACRYGT 3 cut(s) 174, 2042, 5056
AhdI GACNNNNNGTC 1 cut(s) 229
AhlI ACTAGT 1 cut(s) 4105
AjiI CACGTC 1 cut(s) 3609
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AleI CACNNNNGTG 1 cut(s) 2482
Alw21I GWGCWC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
Alw44I GTGCAC 2 cut(s) 4494, 6754
AlwNI CAGNNNCTG 6 cut(s) 920, 2273, 3362, 6030, 6059, 6426
Ama87I CYCGRG 2 cut(s) 227, 275
Aor13HI TCCGGA 1 cut(s) 1525
Aor51HI AGCGCT 1 cut(s) 2918
ApaLI GTGCAC 2 cut(s) 4494, 6754
AseI ATTAAT 4 cut(s) 981, 4812, 4877, 5187
Asp700I GAANNNNTTC 3 cut(s) 2901, 4694, 6126
AspA2I CCTAGG 2 cut(s) 3521, 4736
AspLEI GCGC 7 cut(s) 16, 208, 1542, 2520, 2919, 6250, 6489
AspS9I GGNCC 9 cut(s) 48, 1327, 2858, 3435, 3653, 4534, 4838, 5218, 5932
AsuC2I CCSGG 3 cut(s) 1631, 2282, 4700
AvaI CYCGRG 2 cut(s) 227, 275
AvaII GGWCC 6 cut(s) 48, 2858, 3435, 4838, 5218, 5932
AvrII CCTAGG 2 cut(s) 3521, 4736
AxyI CCTNAGG 1 cut(s) 1383
BaeGI GKGCMC 2 cut(s) 4498, 6758
BaeI ACNNNNGTAYC 4 cut(s) 3243, 3276, 5510, 5543
BalI TGGCCA 3 cut(s) 786, 1683, 4128
BanI GGYRCC 2 cut(s) 2147, 4399
BanII GRGCYC 2 cut(s) 185, 4027
BarI GAAGNNNNNNTAC 2 cut(s) 5289, 5321
BauI CACGAG 2 cut(s) 4938, 6593
BbrPI CACGTG 1 cut(s) 3664
BbsI GAAGAC 5 cut(s) 228, 516, 3702, 4288, 6681
Bbv12I GWGCWC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
BceAI ACGGC 6 cut(s) 388, 479, 3053, 3242, 5984, 6441
BcgI CGANNNNNNTGC 8 cut(s) 1416, 1450, 1893, 1927, 3375, 3409, 5520, 5554
BciVI GTATCC 3 cut(s) 817, 1959, 2501
BclI TGATCA 2 cut(s) 3835, 6289
BcnI CCSGG 3 cut(s) 1631, 2282, 4700
BcuI ACTAGT 1 cut(s) 4105
BfmI CTRYAG 3 cut(s) 848, 5295, 6436
BfoI RGCGCY 3 cut(s) 2920, 6251, 6490
BfrI CTTAAG 2 cut(s) 1331, 4166
BfuAI ACCTGC 3 cut(s) 4849, 5131, 5922
BfuI GTATCC 3 cut(s) 817, 1959, 2501
BglII AGATCT 5 cut(s) 841, 1253, 1715, 1786, 5173
BlnI CCTAGG 2 cut(s) 3521, 4736
BlpI GCTNAGC 1 cut(s) 3015
Bme18I GGWCC 6 cut(s) 48, 2858, 3435, 4838, 5218, 5932
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 2 cut(s) 227, 275
BmgBI CACGTC 1 cut(s) 3609
BmgT120I GGNCC 9 cut(s) 48, 1327, 2858, 3435, 3653, 4534, 4838, 5218, 5932
BmiI GGNNCC 6 cut(s) 373, 1322, 1555, 2149, 4401, 4839
BmrI ACTGGG 1 cut(s) 5950
BmuI ACTGGG 1 cut(s) 5950
BpiI GAAGAC 5 cut(s) 228, 516, 3702, 4288, 6681
BplI GAGNNNNNCTC 2 cut(s) 6558, 6590
BpmI CTGGAG 5 cut(s) 1037, 2574, 2592, 4504, 5268
Bpu10I CCTNAGC 2 cut(s) 4026, 4461
Bpu1102I GCTNAGC 1 cut(s) 3015
BpuEI CTTGAG 7 cut(s) 848, 2648, 4706, 5029, 5101, 5859, 6272
BpuMI CCSGG 3 cut(s) 1631, 2282, 4700
Bsa29I ATCGAT 1 cut(s) 5530
BsaAI YACGTR 3 cut(s) 3382, 3664, 5129
BsaBI GATNNNNATC 2 cut(s) 1305, 1785
BsaHI GRCGYC 1 cut(s) 3229
BsaI GGTCTC 3 cut(s) 64, 3406, 6435
BsaJI CCNNGG 9 cut(s) 1315, 1324, 3521, 3780, 4000, 4247, 4736, 5018, 5972
BsaWI WCCGGW 4 cut(s) 165, 1525, 5463, 6603
BsaXI ACNNNNNCTCC 4 cut(s) 3303, 3333, 4477, 4507
Bse118I RCCGGY 1 cut(s) 6653
Bse21I CCTNAGG 1 cut(s) 1383
Bse3DI GCAATG 7 cut(s) 2877, 3501, 3912, 5510, 5783, 5901, 6436
Bse8I GATNNNNATC 2 cut(s) 1305, 1785
BseAI TCCGGA 1 cut(s) 1525
BseCI ATCGAT 1 cut(s) 5530
BseDI CCNNGG 9 cut(s) 1315, 1324, 3521, 3780, 4000, 4247, 4736, 5018, 5972
BseJI GATNNNNATC 2 cut(s) 1305, 1785
BseMI GCAATG 7 cut(s) 2877, 3501, 3912, 5510, 5783, 5901, 6436
BseRI GAGGAG 1 cut(s) 847
BseSI GKGCMC 2 cut(s) 4498, 6758
BseX3I CGGCCG 1 cut(s) 401
BsgI GTGCAG 5 cut(s) 1170, 3207, 5412, 6016, 6609
Bsh1236I CGCG 5 cut(s) 16, 87, 162, 176, 206
Bsh1285I CGRYCG 2 cut(s) 166, 404
BshNI GGYRCC 2 cut(s) 2147, 4399
BshVI ATCGAT 1 cut(s) 5530
BsiEI CGRYCG 2 cut(s) 166, 404
BsiHKAI GWGCWC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
BsiHKCI CYCGRG 2 cut(s) 227, 275
BsiSI CCGG 8 cut(s) 166, 1526, 1631, 2281, 4699, 5464, 6604, 6654
BsiWI CGTACG 1 cut(s) 3382
BslFI GGGAC 3 cut(s) 2956, 3503, 4851
BsmBI CGTCTC 2 cut(s) 165, 4359
BsmFI GGGAC 3 cut(s) 2956, 3503, 4851
BsmI GAATGC 9 cut(s) 875, 1697, 1916, 2596, 4865, 4873, 4923, 5435, 5797
Bso31I GGTCTC 3 cut(s) 64, 3406, 6435
BsoBI CYCGRG 2 cut(s) 227, 275
Bsp1286I GDGCHC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
Bsp13I TCCGGA 1 cut(s) 1525
Bsp1407I TGTACA 2 cut(s) 1467, 6556
Bsp1720I GCTNAGC 1 cut(s) 3015
Bsp19I CCATGG 1 cut(s) 5018
Bsp68I TCGCGA 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 5530
BspEI TCCGGA 1 cut(s) 1525
BspFNI CGCG 5 cut(s) 16, 87, 162, 176, 206
BspHI TCATGA 3 cut(s) 3988, 4063, 5595
BspLI GGNNCC 6 cut(s) 373, 1322, 1555, 2149, 4401, 4839
BspMAI CTGCAG 1 cut(s) 852
BspMI ACCTGC 3 cut(s) 4849, 5131, 5922
BspQI GCTCTTC 1 cut(s) 5310
BspT107I GGYRCC 2 cut(s) 2147, 4399
BspTI CTTAAG 2 cut(s) 1331, 4166
BspTNI GGTCTC 3 cut(s) 64, 3406, 6435
BsrBI CCGCTC 2 cut(s) 141, 6571
BsrDI GCAATG 7 cut(s) 2877, 3501, 3912, 5510, 5783, 5901, 6436
BsrFI RCCGGY 1 cut(s) 6653
BsrGI TGTACA 2 cut(s) 1467, 6556
BssAI RCCGGY 1 cut(s) 6653
BssECI CCNNGG 9 cut(s) 1315, 1324, 3521, 3780, 4000, 4247, 4736, 5018, 5972
BssNAI GTATAC 2 cut(s) 2489, 4892
BssNI GRCGYC 1 cut(s) 3229
BssSI CACGAG 2 cut(s) 4938, 6593
BssT1I CCWWGG 6 cut(s) 1315, 3521, 3780, 4000, 4736, 5018
Bst1107I GTATAC 2 cut(s) 2489, 4892
Bst2BI CACGAG 2 cut(s) 4938, 6593
Bst6I CTCTTC 5 cut(s) 1850, 3861, 4275, 4316, 5310
BstACI GRCGYC 1 cut(s) 3229
BstAFI CTTAAG 2 cut(s) 1331, 4166
BstAPI GCANNNNNTGC 4 cut(s) 3974, 5803, 6030, 6426
BstAUI TGTACA 2 cut(s) 1467, 6556
BstBAI YACGTR 3 cut(s) 3382, 3664, 5129
BstDSI CCRYGG 1 cut(s) 5018
BstEII GGTNACC 1 cut(s) 3214
BstENI CCTNNNNNAGG 1 cut(s) 4301
BstFNI CGCG 5 cut(s) 16, 87, 162, 176, 206
BstH2I RGCGCY 3 cut(s) 2920, 6251, 6490
BstHHI GCGC 7 cut(s) 16, 208, 1542, 2520, 2919, 6250, 6489
BstMCI CGRYCG 2 cut(s) 166, 404
BstNSI RCATGY 8 cut(s) 857, 2046, 2302, 3300, 4996, 5402, 5435, 6350
BstPI GGTNACC 1 cut(s) 3214
BstSFI CTRYAG 3 cut(s) 848, 5295, 6436
BstSLI GKGCMC 2 cut(s) 4498, 6758
BstSNI TACGTA 1 cut(s) 3382
BstUI CGCG 5 cut(s) 16, 87, 162, 176, 206
BstV2I GAAGAC 5 cut(s) 228, 516, 3702, 4288, 6681
BstX2I RGATCY 9 cut(s) 841, 1253, 1715, 1786, 2427, 2797, 3419, 4580, 5173
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 9 cut(s) 841, 1253, 1715, 1786, 2427, 2797, 3419, 4580, 5173
BstZ17I GTATAC 2 cut(s) 2489, 4892
BstZI CGGCCG 1 cut(s) 401
Bsu15I ATCGAT 1 cut(s) 5530
Bsu36I CCTNAGG 1 cut(s) 1383
BsuI GTATCC 3 cut(s) 817, 1959, 2501
BsuTUI ATCGAT 1 cut(s) 5530
BtgI CCRYGG 1 cut(s) 5018
BtgZI GCGATG 4 cut(s) 793, 4528, 4555, 6255
BtrI CACGTC 1 cut(s) 3609
BtsI GCAGTG 1 cut(s) 3219
BtuMI TCGCGA 1 cut(s) 87
BveI ACCTGC 3 cut(s) 4849, 5131, 5922
CaiI CAGNNNCTG 6 cut(s) 920, 2273, 3362, 6030, 6059, 6426
CciI TCATGA 3 cut(s) 3988, 4063, 5595
CfoI GCGC 7 cut(s) 16, 208, 1542, 2520, 2919, 6250, 6489
Cfr10I RCCGGY 1 cut(s) 6653
Cfr13I GGNCC 9 cut(s) 48, 1327, 2858, 3435, 3653, 4534, 4838, 5218, 5932
ClaI ATCGAT 1 cut(s) 5530
CseI GACGC 5 cut(s) 182, 2207, 3218, 4341, 6686
CspCI CAANNNNNGTGG 2 cut(s) 1531, 1566
DraI TTTAAA 1 cut(s) 906
DraIII CACNNNGTG 1 cut(s) 6762
DrdI GACNNNNNNGTC 3 cut(s) 2267, 3279, 3881
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 3 cut(s) 2267, 3279, 3881
EaeI YGGCCR 4 cut(s) 401, 784, 1681, 4126
EagI CGGCCG 1 cut(s) 401
Eam1104I CTCTTC 5 cut(s) 1850, 3861, 4275, 4316, 5310
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 5 cut(s) 1850, 3861, 4275, 4316, 5310
Ecl136II GAGCTC 2 cut(s) 183, 4025
EclXI CGGCCG 1 cut(s) 401
Eco105I TACGTA 1 cut(s) 3382
Eco130I CCWWGG 6 cut(s) 1315, 3521, 3780, 4000, 4736, 5018
Eco24I GRGCYC 2 cut(s) 185, 4027
Eco31I GGTCTC 3 cut(s) 64, 3406, 6435
Eco32I GATATC 3 cut(s) 490, 5107, 5154
Eco47I GGWCC 6 cut(s) 48, 2858, 3435, 4838, 5218, 5932
Eco47III AGCGCT 1 cut(s) 2918
Eco52I CGGCCG 1 cut(s) 401
Eco53kI GAGCTC 2 cut(s) 183, 4025
Eco72I CACGTG 1 cut(s) 3664
Eco81I CCTNAGG 1 cut(s) 1383
Eco88I CYCGRG 2 cut(s) 227, 275
Eco91I GGTNACC 1 cut(s) 3214
EcoICRI GAGCTC 2 cut(s) 183, 4025
EcoNI CCTNNNNNAGG 1 cut(s) 4301
EcoO109I RGGNCCY 2 cut(s) 1327, 4838
EcoO65I GGTNACC 1 cut(s) 3214
EcoRI GAATTC 3 cut(s) 2901, 4045, 5835
EcoRV GATATC 3 cut(s) 490, 5107, 5154
EcoT14I CCWWGG 6 cut(s) 1315, 3521, 3780, 4000, 4736, 5018
EcoT22I ATGCAT 5 cut(s) 5097, 5400, 5437, 5512, 6348
EcoT38I GRGCYC 2 cut(s) 185, 4027
ErhI CCWWGG 6 cut(s) 1315, 3521, 3780, 4000, 4736, 5018
Esp3I CGTCTC 2 cut(s) 165, 4359
FaqI GGGAC 3 cut(s) 2956, 3503, 4851
FauI CCCGC 3 cut(s) 9, 2295, 3496
FauNDI CATATG 3 cut(s) 1107, 1604, 6159
FbaI TGATCA 2 cut(s) 3835, 6289
FblI GTMKAC 5 cut(s) 2047, 2142, 2488, 4761, 4891
FriOI GRGCYC 2 cut(s) 185, 4027
FspI TGCGCA 2 cut(s) 1541, 2519
GlaI GCGC 7 cut(s) 15, 207, 1541, 2519, 2918, 6249, 6488
GsuI CTGGAG 5 cut(s) 1037, 2574, 2592, 4504, 5268
HaeII RGCGCY 3 cut(s) 2920, 6251, 6490
HapII CCGG 8 cut(s) 166, 1526, 1631, 2281, 4699, 5464, 6604, 6654
HgaI GACGC 5 cut(s) 182, 2207, 3218, 4341, 6686
HhaI GCGC 7 cut(s) 16, 208, 1542, 2520, 2919, 6250, 6489
Hin1I GRCGYC 1 cut(s) 3229
Hin6I GCGC 7 cut(s) 14, 206, 1540, 2518, 2917, 6248, 6487
HinP1I GCGC 7 cut(s) 14, 206, 1540, 2518, 2917, 6248, 6487
HincII GTYRAC 5 cut(s) 2143, 2323, 3616, 4261, 5083
HindII GTYRAC 5 cut(s) 2143, 2323, 3616, 4261, 5083
HindIII AAGCTT 6 cut(s) 654, 1874, 4346, 4704, 5715, 6125
HpaII CCGG 8 cut(s) 166, 1526, 1631, 2281, 4699, 5464, 6604, 6654
Hpy99I CGWCG 2 cut(s) 221, 2147
Hsp92I GRCGYC 1 cut(s) 3229
HspAI GCGC 7 cut(s) 14, 206, 1540, 2518, 2917, 6248, 6487
Kpn2I TCCGGA 1 cut(s) 1525
KroI GCCGGC 1 cut(s) 6653
KroNI GCCGGC 1 cut(s) 6655
Ksp22I TGATCA 2 cut(s) 3835, 6289
LguI GCTCTTC 1 cut(s) 5310
MbiI CCGCTC 2 cut(s) 141, 6571
MfeI CAATTG 4 cut(s) 584, 1763, 2838, 4845
MflI RGATCY 9 cut(s) 841, 1253, 1715, 1786, 2427, 2797, 3419, 4580, 5173
MhlI GDGCHC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
MlsI TGGCCA 3 cut(s) 786, 1683, 4128
MluI ACGCGT 1 cut(s) 174
MluNI TGGCCA 3 cut(s) 786, 1683, 4128
MmeI TCCRAC 4 cut(s) 976, 1198, 1751, 4750
Mox20I TGGCCA 3 cut(s) 786, 1683, 4128
Mph1103I ATGCAT 5 cut(s) 5097, 5400, 5437, 5512, 6348
MroI TCCGGA 1 cut(s) 1525
MroNI GCCGGC 1 cut(s) 6653
MroXI GAANNNNTTC 3 cut(s) 2901, 4694, 6126
MscI TGGCCA 3 cut(s) 786, 1683, 4128
MslI CAYNNNNRTG 8 cut(s) 870, 1932, 2482, 2731, 3750, 3987, 4824, 5017
Msp20I TGGCCA 3 cut(s) 786, 1683, 4128
MspA1I CMGCKG 8 cut(s) 1751, 3090, 3491, 4729, 5102, 6030, 6056, 6683
MspCI CTTAAG 2 cut(s) 1331, 4166
MspI CCGG 8 cut(s) 166, 1526, 1631, 2281, 4699, 5464, 6604, 6654
MunI CAATTG 4 cut(s) 584, 1763, 2838, 4845
Mva1269I GAATGC 9 cut(s) 875, 1697, 1916, 2596, 4865, 4873, 4923, 5435, 5797
MvnI CGCG 5 cut(s) 16, 87, 162, 176, 206
NaeI GCCGGC 1 cut(s) 6655
NciI CCSGG 3 cut(s) 1631, 2282, 4700
NcoI CCATGG 1 cut(s) 5018
NdeI CATATG 3 cut(s) 1107, 1604, 6159
NgoMIV GCCGGC 1 cut(s) 6653
NlaIV GGNNCC 6 cut(s) 373, 1322, 1555, 2149, 4401, 4839
NmeAIII GCCGAG 1 cut(s) 250
NruI TCGCGA 1 cut(s) 87
NsbI TGCGCA 2 cut(s) 1541, 2519
NsiI ATGCAT 5 cut(s) 5097, 5400, 5437, 5512, 6348
NspI RCATGY 8 cut(s) 857, 2046, 2302, 3300, 4996, 5402, 5435, 6350
OliI CACNNNNGTG 1 cut(s) 2482
PaeI GCATGC 4 cut(s) 857, 2302, 4996, 5402
PaeR7I CTCGAG 1 cut(s) 227
PagI TCATGA 3 cut(s) 3988, 4063, 5595
PciI ACATGT 1 cut(s) 2042
PciSI GCTCTTC 1 cut(s) 5310
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 9 cut(s) 875, 1697, 1916, 2596, 4865, 4873, 4923, 5435, 5797
PdiI GCCGGC 1 cut(s) 6655
PdmI GAANNNNTTC 3 cut(s) 2901, 4694, 6126
Pfl23II CGTACG 1 cut(s) 3382
PflFI GACNNNGTC 2 cut(s) 2327, 5591
PflMI CCANNNNNTGG 2 cut(s) 3090, 4135
PfoI TCCNGGA 6 cut(s) 1170, 2032, 2280, 2430, 2662, 4698
PmaCI CACGTG 1 cut(s) 3664
PmlI CACGTG 1 cut(s) 3664
Ppu21I YACGTR 3 cut(s) 3382, 3664, 5129
PpuMI RGGWCCY 1 cut(s) 4838
PscI ACATGT 1 cut(s) 2042
PshBI ATTAAT 4 cut(s) 981, 4812, 4877, 5187
Psp124BI GAGCTC 2 cut(s) 185, 4027
Psp1406I AACGTT 1 cut(s) 4999
Psp5II RGGWCCY 1 cut(s) 4838
PspCI CACGTG 1 cut(s) 3664
PspEI GGTNACC 1 cut(s) 3214
PspLI CGTACG 1 cut(s) 3382
PspN4I GGNNCC 6 cut(s) 373, 1322, 1555, 2149, 4401, 4839
PspPI GGNCC 9 cut(s) 48, 1327, 2858, 3435, 3653, 4534, 4838, 5218, 5932
PspPPI RGGWCCY 1 cut(s) 4838
PspXI VCTCGAGB 1 cut(s) 227
PstI CTGCAG 1 cut(s) 852
PstNI CAGNNNCTG 6 cut(s) 920, 2273, 3362, 6030, 6059, 6426
PsuI RGATCY 9 cut(s) 841, 1253, 1715, 1786, 2427, 2797, 3419, 4580, 5173
PsyI GACNNNGTC 2 cut(s) 2327, 5591
PvuII CAGCTG 6 cut(s) 1751, 4729, 5102, 6030, 6056, 6683
RruI TCGCGA 1 cut(s) 87
RseI CAYNNNNRTG 8 cut(s) 870, 1932, 2482, 2731, 3750, 3987, 4824, 5017
SacI GAGCTC 2 cut(s) 185, 4027
SalI GTCGAC 1 cut(s) 2141
SapI GCTCTTC 1 cut(s) 5310
Sau96I GGNCC 9 cut(s) 48, 1327, 2858, 3435, 3653, 4534, 4838, 5218, 5932
SduI GDGCHC 9 cut(s) 185, 3316, 4027, 4498, 4526, 4821, 4900, 5610, 6758
SfcI CTRYAG 3 cut(s) 848, 5295, 6436
Sfr274I CTCGAG 1 cut(s) 227
SinI GGWCC 6 cut(s) 48, 2858, 3435, 4838, 5218, 5932
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 8 cut(s) 870, 1932, 2482, 2731, 3750, 3987, 4824, 5017
SnaBI TACGTA 1 cut(s) 3382
SpeI ACTAGT 1 cut(s) 4105
SphI GCATGC 4 cut(s) 857, 2302, 4996, 5402
SspI AATATT 4 cut(s) 2082, 3348, 4599, 4749
SstI GAGCTC 2 cut(s) 185, 4027
StyI CCWWGG 6 cut(s) 1315, 3521, 3780, 4000, 4736, 5018
TaqII GACCGA 2 cut(s) 3751, 6098
TatI WGTACW 5 cut(s) 812, 1020, 1467, 2009, 6556
TauI GCSGC 4 cut(s) 75, 144, 156, 6574
TspGWI ACGGA 2 cut(s) 4758, 4917
Tth111I GACNNNGTC 2 cut(s) 2327, 5591
Van91I CCANNNNNTGG 2 cut(s) 3090, 4135
Vha464I CTTAAG 2 cut(s) 1331, 4166
VneI GTGCAC 2 cut(s) 4494, 6754
VpaK11BI GGWCC 6 cut(s) 48, 2858, 3435, 4838, 5218, 5932
VspI ATTAAT 4 cut(s) 981, 4812, 4877, 5187
XagI CCTNNNNNAGG 1 cut(s) 4301
XceI RCATGY 8 cut(s) 857, 2046, 2302, 3300, 4996, 5402, 5435, 6350
XcmI CCANNNNNNNNNTGG 1 cut(s) 5790
XhoI CTCGAG 1 cut(s) 227
XmaJI CCTAGG 2 cut(s) 3521, 4736
XmiI GTMKAC 5 cut(s) 2047, 2142, 2488, 4761, 4891
XmnI GAANNNNTTC 3 cut(s) 2901, 4694, 6126
Zsp2I ATGCAT 5 cut(s) 5097, 5400, 5437, 5512, 6348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.