MD11G1160700.v1.1

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001971
Physical Location & Seq
Forward (+)
15847386 .. 15871863
24478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1160700.v1.1.491

Sequence Viewer

Length: 6660 bp
ATGAGCGGCTACCAGAGCCGAAAGCGGGACCGCAGCGACTCATGGGTCCCCAGGAAGCAGAAGGTCATGCGCACCGCCGAGGAGGAGCTGGAGTCCAAGCTAGGGTTCGACCTGTTCTCCGAAGGCGATACCAGGCTTGGCTGGTTGCTCAATTTCGCCACCTCATCTTGGGAGGATCAAGAGACCCGTAAAGTATATAGTTGTGTCGATTTGTATTTCGTTTCACAGGATGGTTCTACTTTTAAGTCAAAATACAAGTTTCGCCCTTATTTCTATGCAGCAACTAAGGAGAAAATGGAAATGGACGTTGAAGCATATTTGAGACGGCGTTATCAAAATCAAATTGCTGATGTTCAAATAGTGCAGAAAGAAGATCTTGATCTCAAAAATCATTTGTCTGGCCTTCGCAAGTCCTACCTAAAGCTATCATTTGATAACGTTCAACAACTAATGAATGTCAAGAGTGACCTACTGCATGTCGTCGAAAGAAATTGCGCCAAATTTGATGCTGCAGAAGCTTATGAGTCAATATTAATTGGGAAACGAGAACAAAGGACTCAAGATTTTCTAGATTGTATTGTTGATCTTCGTGAATATGATGTTCCATACCATGTTCGCTTTGCCATTGACAATGATGTACGATGCGGGCAGTGGTATGATATTAGTGTATCCAGTACTGGTGCTGTGCTTAAGATGAGGACAGATCTTCTCCAGCGTGCTGAAGTTCATGTCTGTGCATTTGATATTGAGACGACGAAGCTACCTTTGAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGATGGACAAGGTTACTTGATTATTAACAGAGAGTGTGTTGGGGATGACATAGAGGATTTGGAGTACACTCCAAAACCAGAATTTGAAGGGTTTTTCAAGGTTACAAATGTGAAGAATGAGGTAGAGCTTCTAAGAAAATGGTTTTCCCATATGCGGGAGGTGAAGCCTGGAATTTATGTCACATACAATGGTGATTTTTTCGACTGGCCTTTCCTGGAAACCAGAGCCGCGTATCATGGGTTCAAACTGAATGATGAGGTTGGATTTCAATGTGACAAGAATCAGGGGGAATGCCGAGCTAAATTTGCATGCCATCTTGACTGCTTTGCATGGGTCAAACGTGATAGTTATCTTCCTCAAGGAAGCCAGGGCCTTAAGGCTGTTACAAAGGCAAAGTTAGGTTATCATCCGCTTGAGGTAAATCCTGAGGATATGGTTCGCTTTGCAAAGGAGAAGCCTCAGATGATGGCCTCCTATTCTGTTTCTGATGCTGTTGCGACTTATTACTTGTATATGACTTACGTTCATCCATTCATTTTCTCTCTTGCAACTATTATTCCAATGTCTCCGGACGAGGTTTTGCGCAAGGGTAGTGGAACTCTTTGTGAAATGCTTCTCATGGTCCAGGCATACAAGGCAAATGTAATCTGCCCTAACAAACACCAATCTGATCCGGAAAAGTTCTATAAAAATCACCTTTTAGAGAGTGAGACTTATATAGGTGGCCATGTTGAATGCCTTGAAAGTGGTGTTTTTAGATCTGATATTCCAACTAGTTTCAAGCTTGACGCCTCTGCCTATGAGCAATTGATCAACAATCTTGATCGAGATCTTCAATATGCTATAAGAGTAGAGGGTAAGATGGGCTTGGAATCAGTATCCAATTATGAAGATGTGAAGAGTTCTATATTGGAAAAGCTTATGGGGTTGCGAGATGAACCTATACGTGAAGAGTGCCCCCTTATTTATCATCTGGATGTTGCTGCAATGTATCCCAACATTATTTTGACAAACAGACTTCAGCCATCATCAATAGTTACAGACGAGATTTGCACTGCTTGTGATTTCAACCGTCCAGGCAAAACATGTCTTCGCAAGCTTGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAGGAAAAGTGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTTTGTTGACGGTGGCAATTCCAAGCTGTCAAAATCTTTTCTTGAGTTGCCTAAAATGGAACAACAGTCAAGGCTCAAAGAGCGTCTTAAAAAATACTGTCAGAAGGCGTATAGACGAGTAGTGGACAAGCCAGTCACTGAAGTTCGAGAAGCAGGGATATGCATGAGGGAAAATTCATTTTATGTTGATACAGTTCGTAGCTTTCGAGATAGAAGGTATGAGTACAAAGGGCTTAATAAGGTTTGGAAGGGGAAGTTGTCTGAAGCCAAGGCTAGTGGAAATTCTATAAAAATCCAGGAAGCACAAGATATGGTAGTGCTTTATGATTCATTACAACTTGCACATAAATGTATACTTAATTCCTTTTATGGATATGTCATGCGCAAGGGTGCAAGATGGTATTCCATGGAAATGGCTGGAGTAGTTACATATACCGGAGCCAAAATTATTCAGAATGCTCGCTTGCTGGTTGAGAAAATTGGAAAACCTCTCGAGTTAGACACAGATGGTATCTGGTGTGCACTCCCTGGATCTTTTCCGGAGAACTTTACATTCAAAACAAAAGACATGAAGAAGCTGACTATCTCATATCCTTGTGTAATGCTTAACGTTGATGTGGCAAGAAACAATACAAATGACCAATACCAGACTCTTACAGATCCTGTTAAGAATACTTACACAACTCATAGCGAGTGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTCTTCCTGCTTCTAAAGAAGAAGGCATTCTAATTAAGAAGCGATATGCTGTTTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTCGAGATCAAGCGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAACTTTTTGAGAAGTTCCTTCACGGTTCTACTTTAGAGGAATGCTATTTTGCAGTTGCTTCTGTCGCAAACCGTTGGCTTGATCTCCTTGATAATCAAGGAAAGGATATTGCAGACAGTGAGTTGCTGGATTATATATCAGAGTCAAGCACCATGAGCAAGTCTTTAGTAGACTATGGAGAGCAAAAGTCATGCGCAGTGACCACTGCAAGACGTCTTGCTGATTTTCTTGGTGATACGATGGTCAAAGAGAAGGGACTGCGTTGCCAATATATAGTTGCATGTGAACCAAAGGGCACACCTGTTAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTATGTAAGGAAGTGGTGCAAGGTTTCATCAGATGTTGGCATTCGTTCCATTATTGACTGGTCCTATTACAAGCAACGCCTTGGTTCAGCAATTCAGAAAATTGTCACCATTCCGGCTGCAATGCAAAAGGTTGCAAATCCTGTTCCTAGGGTAGCTCATCCTGATTGGCTGCATAAGAAGGTTCGTGAGAAGGACGACAAATTTCGCCAGCGCAAGTTGGTTGATATCTTCAAGTCATTGAACAGGGATCATTTGCTAGAAAAGAGCAGTGATGCTATTGACTCTAATCATGTGATTGACCAAGAGATTGTGGATGATTTGGAAGACTTCAACAAAACAAGCAGTTCCATAAGTGGACCCAGACCAATTGTTCGATGTTATGAAGTCAATAGCAAGAAATATTCAGTCGAGACAACTTGCCAAATAAATTCACAGCAACAACAAACTAATAATAAAGAAAATGAGTGTCAAGAGCAAAATGGTGTCCCTTTAGAATGCATTGATAGAAATGTTGATTATAATGGTTGGCTTGAAATGAAGAAGAGAAAATGGAAAGATACTCTTGACAGGAGGAAGAAGCAAAGATCAAATAACGCAAGGTCTGCTCACCATGCTAATAATGAGCTGCATGGTGGTGGTAGAAAGCAGAAAGGAACTCAGGGAAAAATAGGTGTGAATTCATATTTTAGGAGGCATGAAGATGCCCTAACACGCTGTCATTGGCAGATATTACAACTAGTGCCTAGTTCTCAGGGTGACCAATTTTACGCTTGGGTGGTTACTGAAGGAATCATGATTAAGATTCCTGTTACTGTTCCAAGAGTTTTTTACATTAATTCTAAAGCACCTATTGAAAAGATATTTGAAAAGATATGTGAAGAAATACCTCCAGGAAAGGATGTCAAAAAGGCTCTTCCTCATGGAAGGCAGATCTACCACCTATACGAGGCTGCAATTGATGAGGGTCGGTTTAGGACAATTAGTAAAAAGCTTGCAGCTCTTCTCGGCGATCCAGAAGTTGAGGGAATATATGAAACAAAGGTACCTTTGGAGTTCAATGCAATCGTTCAGATTGGTTGTGTCTGCAAAGTGGATAAAACTGCTAAGAAGAGAAACGTCCAAGATGGTTGGACTGTGAGTGAACTGCACATGAAGACAACTACAGAGTGCTCATATCTTGACCAGTCCATCTCATTCTTTTACTTGTATCAGAGCACATCTGAAGGGCGAGCTATATATATTGCCTATTTTCCTGCATCGGCAACAATAACGGTTGTGGTGGTAAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTGGAAAAACTATTCCGTGATGCATGCCAAGCATTATCAATTCAGCCACCTCCTAGGAATGGTATCGTTATAAAGGTGGACTATGTTGGATTCGTCAAGGATGCTGAAACAATTTTGCACCGTGCAATTAATGAGTACAGACATGAACATTCTGGACCTACAATTGCTGTTATCGAATGCCCAAATTTTCAATTGATGAAGTCAGGTATAGGAGCTCTTGATGATTTTCCTTGTGTGAGCATTCCCTCTAATGCTCGTGATAGTCTTTACCAGGTTCTTGGGTGGCAGCAAGTGGCTGCAAAAATTGGAATGCAGCGCTGTGCTGCATCACCTCAGTGGTTGAATGAGAGAATTTCACTCTCAAGATATGCTCATGTTCCATTGGGAAATTTCGAACTGGACTGGCTTATACATACAGCAGATATCTTCTTTTCAAGAGCACTACGTGATCAACAACAGGTGCTTTGGATATCTGATGATGGTATTCCAGATCTAGGAGGCATTAATGAAGAAGATTCATGTTTTGCTGATGAGGTCTGTGAACTTAATTTTTCTTACCCTGGAGCATATAGGAAGGTCACCGTGGAGTTAAAGATACATCATCTGGCTGTAAATGCTCTCTTAAAAAGCAACCAGGTGAATGAAATTGAAGGAGGTGCTTTGCTAGGATTTGAATCAGACGTGAACTTGGGAGTACATACTTTTGATGCACATGTTGGCTTTGATGAAGCTAAATCATGTGCTCCTACATTCCGAGTCCTGAAACAGTTGATTCAGAGGTGTCTTTCAAATGCAGTTACGTCTGGAAATGTTTACGCCGATGCATTATTGCAACATTTATATCGATGGCTTTGCAGCCCCCAGTCTAAGCTTCATGATCCAGCTCTTCACCGCATACTTCATAAGGTCATGAAAAAGGTGCTTGCAATTTTAATGACTGAGTTCCGCAAATTGGGTGCCAAGATAATATTCGTGAATTTCTACAAGGTCATTATAGACACAGGAAAATTTGATCTGTCAGCTGCCAAAGCTTATTGTGATAGCTTGCTTAAAACTCTGCAAACGAGAGAACTATTTGAGTGGATTGAGCTTGAACCACTACACTTCTGGCATTCTTTTCTTTTCATGGACCAGTATAACTATGGTGGAATTCCGGTTAGAGATGAAGGAAGCATGGATGGTGAAGCTCAAATGGATATTGTATCAAGCTGGAATATTGCTGAATGCTTACCCAAGAAAATTCAGGACCACTTTGTTTATATTGTATCTCAATTTCTGTATATTCCCTGGGACTATGCACAAAAGCAGGCAGCAGTTAGAACATCTTTACAGAATGACAGCATTTGCACGCCATCAATCACTGTAGCAGATGCTGAAACTTTTGAGTCTCATATGATAGATTTCCTTAAAGGACAGATCAGCTCATATTTTACTGATAAACTTCTGGGAATTGTTCATGATACGATACTTCATGTGAAGGAACTGAAAAAATCAGAGAATGACCATGATACATCTCTTGGGCTGCCACAAATTGCTGGTAATATCCACCAAGGGGATGCAGCATTGGAGTTCATTAAGCACGTCTGCGGTGTTTTCTCCATTGACCAAAATGTTATCCATGATGTTCTGGTAATGAGAAAGAATCTGTTGAAGATTGTACGTGTGAGGGAGTTTGCCCCTGAAGCAGAGTTTCGTGATCCCGGCGCAACCTTCACTTTACCAAATGTTATTTGCAGCTACTGCAACGACTGTAGAGACCTGGACTTATGCCGCGACTCAGCTCTTCTAGCCAGGGAGTGGCGTTGTTGTGTACCACAATGTGGGCAGCTTTATGACAAAGAGGTGATGGAGAATGCCCTTCTTCAGATTGTGCGGCAGAGAGAGCGTCTCTACCAACTACAGGATCTCCAATGCCTCAGGTGTCACCAAGTGAAAGCTGCACGTTTAGCAGAGCAATGTGTATGCGCTGGCTCATATAAGTGCAGGCAAAACGCAACTGAATTTCGTACCCAAATGGAGTTAATTTGGAAGATTGCCATTCATCAGAAATTTCAACTTCTCCAAGAGTGCATTGAATGGATTTTAGAAACGAACAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2220

Amino Acids

254.78

Weight (kDa)

6.5

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 195 - 401 1.3e-56 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 755 - 1071 1.8e-17 DNA polymerase family B
POL2_thumb PF22634 946 - 1121 1.2e-76 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1539 - 1903 4.4e-113 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 2080 - 2146 3.6e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2151 - 2208 6.6e-13 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3756, 4628
AasI GACNNNNNNGTC 2 cut(s) 44, 2129
AatII GACGTC 1 cut(s) 3091
Acc16I TGCGCA 4 cut(s) 71, 1403, 2381, 3070
Acc65I GGTACC 1 cut(s) 4279
AccB1I GGYRCC 2 cut(s) 4279, 5513
AccB7I CCANNNNNTGG 2 cut(s) 6354, 6377
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 2350, 3045
AccII CGCG 2 cut(s) 1049, 6330
AccIII TCCGGA 3 cut(s) 1387, 1492, 2536
AclI AACGTT 2 cut(s) 438, 2607
AclWI GGATC 9 cut(s) 183, 1484, 2536, 2651, 3492, 4241, 5429, 6248, 6468
AcoI YGGCCR 1 cut(s) 1543
AcuI CTGAAG 9 cut(s) 741, 1823, 2157, 2280, 2870, 4041, 4479, 6258, 6404
AcyI GRCGYC 2 cut(s) 1608, 3088
AdeI CACNNNGTG 3 cut(s) 5003, 6377, 6487
AfeI AGCGCT 1 cut(s) 4874
AflII CTTAAG 2 cut(s) 689, 1193
AflIII ACRYGT 3 cut(s) 1904, 5269, 6217
AhlI ACTAGT 2 cut(s) 1592, 3973
AjiI CACGTC 2 cut(s) 5239, 6139
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 5510, 5542
AleI CACNNNNGTG 1 cut(s) 4891
Alw21I GWGCWC 6 cut(s) 2521, 4409, 4454, 4774, 4999, 5302
Alw26I GTCTC 9 cut(s) 176, 316, 743, 1389, 1523, 3641, 5949, 6306, 6449
Alw44I GTGCAC 1 cut(s) 2517
AlwI GGATC 9 cut(s) 183, 1484, 2536, 2651, 3492, 4241, 5429, 6248, 6468
AlwNI CAGNNNCTG 6 cut(s) 782, 2135, 2660, 3221, 5930, 6297
Ama87I CYCGRG 1 cut(s) 2489
Aor13HI TCCGGA 3 cut(s) 1387, 1492, 2536
Aor51HI AGCGCT 1 cut(s) 4874
AoxI GGCC 5 cut(s) 400, 1025, 1189, 1287, 1543
ApaLI GTGCAC 1 cut(s) 2517
ArsI GACNNNNNNTTYG 2 cut(s) 3591, 3623
AseI ATTAAT 4 cut(s) 533, 4071, 4686, 5061
Asp700I GAANNNNTTC 2 cut(s) 1431, 2760
Asp718I GGTACC 1 cut(s) 4279
AspA2I CCTAGG 2 cut(s) 3383, 4610
AspLEI GCGC 9 cut(s) 72, 497, 1404, 2382, 3071, 3450, 4875, 6263, 6524
AsuC2I CCSGG 1 cut(s) 6258
AsuII TTCGAA 1 cut(s) 4950
AvaI CYCGRG 1 cut(s) 2489
AvrII CCTAGG 2 cut(s) 3383, 4610
AxyI CCTNAGG 2 cut(s) 1245, 6473
BaeGI GKGCMC 3 cut(s) 1778, 2521, 3173
BaeI ACNNNNGTAYC 4 cut(s) 3102, 3135, 4263, 4296
BalI TGGCCA 1 cut(s) 1545
BanI GGYRCC 2 cut(s) 4279, 5513
BanII GRGCYC 1 cut(s) 4774
BauI CACGAG 1 cut(s) 4812
BbsI GAAGAC 3 cut(s) 1901, 3567, 4397
Bbv12I GWGCWC 6 cut(s) 2521, 4409, 4454, 4774, 4999, 5302
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 5391, 5425
BciVI GTATCC 3 cut(s) 679, 1708, 1821
BclI TGATCA 2 cut(s) 1629, 5005
BcnI CCSGG 1 cut(s) 6258
BcoDI GTCTC 9 cut(s) 176, 316, 743, 1389, 1523, 3641, 5949, 6306, 6449
BcuI ACTAGT 2 cut(s) 1592, 3973
BfmI CTRYAG 5 cut(s) 510, 4398, 5919, 6307, 6455
BfoI RGCGCY 1 cut(s) 4876
BfrI CTTAAG 2 cut(s) 689, 1193
BfuI GTATCC 3 cut(s) 679, 1708, 1821
BglII AGATCT 6 cut(s) 373, 703, 1577, 1648, 4167, 5047
BlnI CCTAGG 2 cut(s) 3383, 4610
BmcAI AGTACT 1 cut(s) 676
BmeT110I CYCGRG 1 cut(s) 2489
BmgBI CACGTC 2 cut(s) 5239, 6139
BmiI GGNNCC 9 cut(s) 29, 47, 48, 2437, 2803, 2804, 3595, 4281, 5515
BmrI ACTGGG 1 cut(s) 5413
BmuI ACTGGG 1 cut(s) 5413
BpiI GAAGAC 3 cut(s) 1901, 3567, 4397
BplI GAGNNNNNCTC 2 cut(s) 6429, 6461
BpmI CTGGAG 5 cut(s) 110, 695, 2436, 4110, 5139
Bpu14I TTCGAA 1 cut(s) 4950
BpuEI CTTGAG 5 cut(s) 543, 1161, 1253, 2060, 4903
BpuMI CCSGG 1 cut(s) 6258
Bsa29I ATCGAT 1 cut(s) 5401
BsaAI YACGTR 3 cut(s) 1766, 5003, 6218
BsaBI GATNNNNATC 4 cut(s) 378, 1167, 1647, 5230
BsaHI GRCGYC 2 cut(s) 1608, 3088
BsaI GGTCTC 2 cut(s) 176, 6306
BsaWI WCCGGW 5 cut(s) 1387, 1492, 2432, 2536, 5710
BsaXI ACNNNNNCTCC 6 cut(s) 77, 101, 107, 131, 6447, 6477
Bse21I CCTNAGG 2 cut(s) 1245, 6473
Bse3DI GCAATG 4 cut(s) 1812, 2736, 3363, 6518
Bse8I GATNNNNATC 4 cut(s) 378, 1167, 1647, 5230
BseAI TCCGGA 3 cut(s) 1387, 1492, 2536
BseCI ATCGAT 1 cut(s) 5401
BseJI GATNNNNATC 4 cut(s) 378, 1167, 1647, 5230
BseMI GCAATG 4 cut(s) 1812, 2736, 3363, 6518
BseMII CTCAG 9 cut(s) 1236, 1292, 1986, 3908, 4001, 4904, 5487, 6348, 6487
BseRI GAGGAG 2 cut(s) 95, 98
BseSI GKGCMC 3 cut(s) 1778, 2521, 3173
BsgI GTGCAG 4 cut(s) 383, 4367, 6480, 6559
Bsh1236I CGCG 2 cut(s) 1049, 6330
BshFI GGCC 5 cut(s) 402, 1027, 1191, 1289, 1545
BshNI GGYRCC 2 cut(s) 4279, 5513
BshVI ATCGAT 1 cut(s) 5401
BsiHKAI GWGCWC 6 cut(s) 2521, 4409, 4454, 4774, 4999, 5302
BsiHKCI CYCGRG 1 cut(s) 2489
BsiSI CCGG 7 cut(s) 1388, 1493, 2433, 2537, 3350, 5711, 6258
BslFI GGGAC 6 cut(s) 32, 41, 2815, 3144, 3707, 5861
BsmAI GTCTC 9 cut(s) 176, 316, 743, 1389, 1523, 3641, 5949, 6306, 6449
BsmBI CGTCTC 3 cut(s) 316, 743, 6449
BsmFI GGGAC 6 cut(s) 32, 41, 2815, 3144, 3707, 5861
BsnI GGCC 5 cut(s) 402, 1027, 1191, 1289, 1545
Bso31I GGTCTC 2 cut(s) 176, 6306
BsoBI CYCGRG 1 cut(s) 2489
Bsp119I TTCGAA 1 cut(s) 4950
Bsp1286I GDGCHC 8 cut(s) 1778, 2521, 3173, 4409, 4454, 4774, 4999, 5302
Bsp13I TCCGGA 3 cut(s) 1387, 1492, 2536
Bsp19I CCATGG 1 cut(s) 2403
BspANI GGCC 5 cut(s) 402, 1027, 1191, 1289, 1545
BspCNI CTCAG 9 cut(s) 1237, 1291, 1987, 3907, 4000, 4903, 5488, 6347, 6486
BspDI ATCGAT 1 cut(s) 5401
BspEI TCCGGA 3 cut(s) 1387, 1492, 2536
BspFNI CGCG 2 cut(s) 1049, 6330
BspHI TCATGA 4 cut(s) 4029, 5431, 5466, 6013
BspLI GGNNCC 9 cut(s) 29, 47, 48, 2437, 2803, 2804, 3595, 4281, 5515
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 9 cut(s) 183, 1484, 2536, 2651, 3492, 4241, 5429, 6248, 6468
BspQI GCTCTTC 4 cut(s) 4155, 4242, 5448, 6345
BspT104I TTCGAA 1 cut(s) 4950
BspT107I GGYRCC 2 cut(s) 4279, 5513
BspTI CTTAAG 2 cut(s) 689, 1193
BspTNI GGTCTC 2 cut(s) 176, 6306
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 1812, 2736, 3363, 6518
BssNAI GTATAC 1 cut(s) 2351
BssNI GRCGYC 2 cut(s) 1608, 3088
BssSI CACGAG 1 cut(s) 4812
BssT1I CCWWGG 6 cut(s) 2265, 2403, 3316, 3383, 4610, 6106
Bst1107I GTATAC 1 cut(s) 2351
Bst2BI CACGAG 1 cut(s) 4812
Bst6I CTCTTC 8 cut(s) 1712, 1764, 3773, 4155, 4242, 4340, 5448, 6345
BstACI GRCGYC 2 cut(s) 1608, 3088
BstAFI CTTAAG 2 cut(s) 689, 1193
BstAPI GCANNNNNTGC 2 cut(s) 3839, 6297
BstBAI YACGTR 3 cut(s) 1766, 5003, 6218
BstBI TTCGAA 1 cut(s) 4950
BstDSI CCRYGG 2 cut(s) 2403, 5139
BstEII GGTNACC 2 cut(s) 3992, 5134
BstENI CCTNNNNNAGG 1 cut(s) 4181
BstFNI CGCG 2 cut(s) 1049, 6330
BstH2I RGCGCY 1 cut(s) 4876
BstHHI GCGC 9 cut(s) 72, 497, 1404, 2382, 3071, 3450, 4875, 6263, 6524
BstMAI GTCTC 9 cut(s) 176, 316, 743, 1389, 1523, 3641, 5949, 6306, 6449
BstNSI RCATGY 6 cut(s) 479, 1131, 1908, 3159, 4584, 5273
BstPI GGTNACC 2 cut(s) 3992, 5134
BstSFI CTRYAG 5 cut(s) 510, 4398, 5919, 6307, 6455
BstSLI GKGCMC 3 cut(s) 1778, 2521, 3173
BstUI CGCG 2 cut(s) 1049, 6330
BstV2I GAAGAC 3 cut(s) 1901, 3567, 4397
BstX2I RGATCY 9 cut(s) 373, 703, 1577, 1648, 2528, 2656, 4167, 5047, 6460
BstXI CCANNNNNNTGG 2 cut(s) 2410, 4835
BstYI RGATCY 9 cut(s) 373, 703, 1577, 1648, 2528, 2656, 4167, 5047, 6460
BstZ17I GTATAC 1 cut(s) 2351
Bsu15I ATCGAT 1 cut(s) 5401
Bsu36I CCTNAGG 2 cut(s) 1245, 6473
BsuI GTATCC 3 cut(s) 679, 1708, 1821
BsuRI GGCC 5 cut(s) 402, 1027, 1191, 1289, 1545
BsuTUI ATCGAT 1 cut(s) 5401
BtgI CCRYGG 2 cut(s) 2403, 5139
BtrI CACGTC 2 cut(s) 5239, 6139
BtsI GCAGTG 5 cut(s) 656, 1872, 3078, 3078, 3511
BtsIMutI CAGTG 9 cut(s) 656, 1872, 2133, 2998, 3078, 3078, 3511, 4898, 5916
CaiI CAGNNNCTG 6 cut(s) 782, 2135, 2660, 3221, 5930, 6297
CciI TCATGA 4 cut(s) 4029, 5431, 5466, 6013
CfoI GCGC 9 cut(s) 72, 497, 1404, 2382, 3071, 3450, 4875, 6263, 6524
ClaI ATCGAT 1 cut(s) 5401
CseI GACGC 3 cut(s) 1616, 2069, 6431
CsiI ACCWGGT 2 cut(s) 4827, 5190
CspCI CAANNNNNGTGG 4 cut(s) 1393, 1428, 2254, 2289
DraIII CACNNNGTG 3 cut(s) 5003, 6377, 6487
DrdI GACNNNNNNGTC 2 cut(s) 44, 2129
DseDI GACNNNNNNGTC 2 cut(s) 44, 2129
EaeI YGGCCR 1 cut(s) 1543
Eam1104I CTCTTC 8 cut(s) 1712, 1764, 3773, 4155, 4242, 4340, 5448, 6345
EarI CTCTTC 8 cut(s) 1712, 1764, 3773, 4155, 4242, 4340, 5448, 6345
Ecl136II GAGCTC 1 cut(s) 4772
Eco130I CCWWGG 6 cut(s) 2265, 2403, 3316, 3383, 4610, 6106
Eco24I GRGCYC 1 cut(s) 4774
Eco31I GGTCTC 2 cut(s) 176, 6306
Eco32I GATATC 3 cut(s) 3463, 4981, 5028
Eco47III AGCGCT 1 cut(s) 4874
Eco53kI GAGCTC 1 cut(s) 4772
Eco57I CTGAAG 9 cut(s) 741, 1823, 2157, 2280, 2870, 4041, 4479, 6258, 6404
Eco81I CCTNAGG 2 cut(s) 1245, 6473
Eco88I CYCGRG 1 cut(s) 2489
Eco91I GGTNACC 2 cut(s) 3992, 5134
EcoICRI GAGCTC 1 cut(s) 4772
EcoNI CCTNNNNNAGG 1 cut(s) 4181
EcoO109I RGGNCCY 3 cut(s) 46, 1189, 2802
EcoO65I GGTNACC 2 cut(s) 3992, 5134
EcoRI GAATTC 2 cut(s) 3913, 5706
EcoRV GATATC 3 cut(s) 3463, 4981, 5028
EcoT14I CCWWGG 6 cut(s) 2265, 2403, 3316, 3383, 4610, 6106
EcoT22I ATGCAT 4 cut(s) 2162, 3737, 4582, 5383
EcoT38I GRGCYC 1 cut(s) 4774
ErhI CCWWGG 6 cut(s) 2265, 2403, 3316, 3383, 4610, 6106
Esp3I CGTCTC 3 cut(s) 316, 743, 6449
FalI AAGNNNNNCTT 8 cut(s) 360, 392, 1670, 1702, 2067, 2099, 3783, 3815
FaqI GGGAC 6 cut(s) 32, 41, 2815, 3144, 3707, 5861
FauI CCCGC 3 cut(s) 18, 638, 966
FauNDI CATATG 2 cut(s) 969, 5949
FbaI TGATCA 2 cut(s) 1629, 5005
FblI GTMKAC 2 cut(s) 2350, 3045
FriOI GRGCYC 1 cut(s) 4774
FspAI RTGCGCAY 1 cut(s) 71
FspI TGCGCA 4 cut(s) 71, 1403, 2381, 3070
GlaI GCGC 9 cut(s) 71, 496, 1403, 2381, 3070, 3449, 4874, 6262, 6523
GsuI CTGGAG 5 cut(s) 110, 695, 2436, 4110, 5139
HaeII RGCGCY 1 cut(s) 4876
HaeIII GGCC 5 cut(s) 402, 1027, 1191, 1289, 1545
HapII CCGG 7 cut(s) 1388, 1493, 2433, 2537, 3350, 5711, 6258
HgaI GACGC 3 cut(s) 1616, 2069, 6431
HhaI GCGC 9 cut(s) 72, 497, 1404, 2382, 3071, 3450, 4875, 6263, 6524
Hin1I GRCGYC 2 cut(s) 1608, 3088
Hin6I GCGC 9 cut(s) 70, 495, 1402, 2380, 3069, 3448, 4873, 6261, 6522
HinP1I GCGC 9 cut(s) 70, 495, 1402, 2380, 3069, 3448, 4873, 6261, 6522
HincII GTYRAC 1 cut(s) 2005
HindII GTYRAC 1 cut(s) 2005
HindIII AAGCTT 7 cut(s) 516, 1601, 1736, 1916, 4226, 5426, 5586
HpaII CCGG 7 cut(s) 1388, 1493, 2433, 2537, 3350, 5711, 6258
Hpy99I CGWCG 2 cut(s) 485, 757
Hsp92I GRCGYC 2 cut(s) 1608, 3088
HspAI GCGC 9 cut(s) 70, 495, 1402, 2380, 3069, 3448, 4873, 6261, 6522
KflI GGGWCCC 2 cut(s) 46, 2802
Kpn2I TCCGGA 3 cut(s) 1387, 1492, 2536
KpnI GGTACC 1 cut(s) 4283
Ksp22I TGATCA 2 cut(s) 1629, 5005
LguI GCTCTTC 4 cut(s) 4155, 4242, 5448, 6345
LmnI GCTCC 5 cut(s) 85, 2435, 4769, 5120, 5305
MabI ACCWGGT 2 cut(s) 4827, 5190
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 6 cut(s) 1625, 2697, 3603, 4191, 4719, 4748
MflI RGATCY 9 cut(s) 373, 703, 1577, 1648, 2528, 2656, 4167, 5047, 6460
MhlI GDGCHC 8 cut(s) 1778, 2521, 3173, 4409, 4454, 4774, 4999, 5302
MlsI TGGCCA 1 cut(s) 1545
MluNI TGGCCA 1 cut(s) 1545
MmeI TCCRAC 4 cut(s) 1060, 1613, 4346, 4624
Mox20I TGGCCA 1 cut(s) 1545
Mph1103I ATGCAT 4 cut(s) 2162, 3737, 4582, 5383
MroI TCCGGA 3 cut(s) 1387, 1492, 2536
MroXI GAANNNNTTC 2 cut(s) 1431, 2760
MscI TGGCCA 1 cut(s) 1545
MslI CAYNNNNRTG 8 cut(s) 732, 1794, 2344, 2408, 3870, 3936, 4891, 6535
Msp20I TGGCCA 1 cut(s) 1545
MspA1I CMGCKG 1 cut(s) 5579
MspCI CTTAAG 2 cut(s) 689, 1193
MspI CCGG 7 cut(s) 1388, 1493, 2433, 2537, 3350, 5711, 6258
MunI CAATTG 6 cut(s) 1625, 2697, 3603, 4191, 4719, 4748
MvnI CGCG 2 cut(s) 1049, 6330
NciI CCSGG 1 cut(s) 6258
NcoI CCATGG 1 cut(s) 2403
NdeI CATATG 2 cut(s) 969, 5949
NlaIV GGNNCC 9 cut(s) 29, 47, 48, 2437, 2803, 2804, 3595, 4281, 5515
NmeAIII GCCGAG 3 cut(s) 103, 1139, 4221
NsbI TGCGCA 4 cut(s) 71, 1403, 2381, 3070
NsiI ATGCAT 4 cut(s) 2162, 3737, 4582, 5383
NspI RCATGY 6 cut(s) 479, 1131, 1908, 3159, 4584, 5273
NspV TTCGAA 1 cut(s) 4950
OliI CACNNNNGTG 1 cut(s) 4891
PaeI GCATGC 2 cut(s) 1131, 4584
PaeR7I CTCGAG 1 cut(s) 2489
PagI TCATGA 4 cut(s) 4029, 5431, 5466, 6013
PasI CCCWGGG 1 cut(s) 5844
PciI ACATGT 2 cut(s) 1904, 5269
PciSI GCTCTTC 4 cut(s) 4155, 4242, 5448, 6345
PcsI WCGNNNNNNNCGW 1 cut(s) 2200
PdmI GAANNNNTTC 2 cut(s) 1431, 2760
PflMI CCANNNNNTGG 2 cut(s) 6354, 6377
PfoI TCCNGGA 3 cut(s) 1032, 2292, 4126
Ppu21I YACGTR 3 cut(s) 1766, 5003, 6218
PpuMI RGGWCCY 2 cut(s) 46, 2802
PscI ACATGT 2 cut(s) 1904, 5269
PshBI ATTAAT 4 cut(s) 533, 4071, 4686, 5061
PsiI TTATAA 2 cut(s) 3756, 4628
Psp124BI GAGCTC 1 cut(s) 4774
Psp1406I AACGTT 2 cut(s) 438, 2607
Psp5II RGGWCCY 2 cut(s) 46, 2802
PspEI GGTNACC 2 cut(s) 3992, 5134
PspN4I GGNNCC 9 cut(s) 29, 47, 48, 2437, 2803, 2804, 3595, 4281, 5515
PspPPI RGGWCCY 2 cut(s) 46, 2802
PsrI GAACNNNNNNTAC 2 cut(s) 1043, 1075
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 6 cut(s) 782, 2135, 2660, 3221, 5930, 6297
PsuI RGATCY 9 cut(s) 373, 703, 1577, 1648, 2528, 2656, 4167, 5047, 6460
PvuII CAGCTG 1 cut(s) 5579
RseI CAYNNNNRTG 8 cut(s) 732, 1794, 2344, 2408, 3870, 3936, 4891, 6535
SacI GAGCTC 1 cut(s) 4774
SapI GCTCTTC 4 cut(s) 4155, 4242, 5448, 6345
ScaI AGTACT 1 cut(s) 676
SduI GDGCHC 8 cut(s) 1778, 2521, 3173, 4409, 4454, 4774, 4999, 5302
SexAI ACCWGGT 2 cut(s) 4827, 5190
SfcI CTRYAG 5 cut(s) 510, 4398, 5919, 6307, 6455
Sfr274I CTCGAG 1 cut(s) 2489
SfuI TTCGAA 1 cut(s) 4950
SlaI CTCGAG 1 cut(s) 2489
SmiMI CAYNNNNRTG 8 cut(s) 732, 1794, 2344, 2408, 3870, 3936, 4891, 6535
SmlI CTYRAG 8 cut(s) 558, 689, 1176, 1193, 1232, 2039, 2489, 4918
SmoI CTYRAG 8 cut(s) 558, 689, 1176, 1193, 1232, 2039, 2489, 4918
SpeI ACTAGT 2 cut(s) 1592, 3973
SphI GCATGC 2 cut(s) 1131, 4584
SspI AATATT 6 cut(s) 531, 1944, 3207, 3638, 5526, 5773
SstI GAGCTC 1 cut(s) 4774
StyI CCWWGG 6 cut(s) 2265, 2403, 3316, 3383, 4610, 6106
TatI WGTACW 5 cut(s) 674, 882, 2220, 4692, 5251
TauI GCSGC 4 cut(s) 9, 1049, 6330, 6433
TscAI CASTG 9 cut(s) 656, 1879, 2140, 2998, 3078, 3085, 3511, 4898, 5923
TspGWI ACGGA 1 cut(s) 4562
TspRI CASTG 9 cut(s) 656, 1879, 2140, 2998, 3078, 3085, 3511, 4898, 5923
Van91I CCANNNNNTGG 2 cut(s) 6354, 6377
Vha464I CTTAAG 2 cut(s) 689, 1193
VneI GTGCAC 1 cut(s) 2517
VspI ATTAAT 4 cut(s) 533, 4071, 4686, 5061
XagI CCTNNNNNAGG 1 cut(s) 4181
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 6 cut(s) 479, 1131, 1908, 3159, 4584, 5273
XcmI CCANNNNNNNNNTGG 2 cut(s) 5661, 5696
XhoI CTCGAG 1 cut(s) 2489
XmaJI CCTAGG 2 cut(s) 3383, 4610
XmiI GTMKAC 2 cut(s) 2350, 3045
XmnI GAANNNNTTC 2 cut(s) 1431, 2760
ZraI GACGTC 1 cut(s) 3089
ZrmI AGTACT 1 cut(s) 676
Zsp2I ATGCAT 4 cut(s) 2162, 3737, 4582, 5383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.