Rroxscaffold_1G00026150

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001971
Physical Location & Seq
Reverse (-)
32861896 .. 32869104
7209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026150.1

Sequence Viewer

Length: 1341 bp
ATGGTGATCGTGCGGGAATGCTATTCAGCAGTTGCTTTTGTTGCCAACCGCTGGCTTGATCTTCTTGATAATCAAGGAAAGGATATAGCAGACAGTGAGTTGCTTGATTATATATCAGAATCAAGCACCATGAGCAAGTCTTTTGCGGAATATGGAAGCCAAAAGTCATGTGCAGTAACCACTGCAACGCATCTTGCTGATTTTCTTGATAGGATCACTCTACGAACACATGTCAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTATGTACGGAAGTGGTGCAAGGTCTCTGATGTTGACATCAAATCCATTATAGACTGGTCCTATTACAAACAACGTCTAGATGAAGATATTGTTGAAGTTTTGGAAGACTTCAAATGCAAAAGCAGTTCCATAAGTAGACCGAGACCAATTGTTCACTGTTATGAAGTTAATAACCAGAAAAATTCAGTAAAGGCAAATGGTAAAGGAGGTCCAGAGCAACAACAAACTAATCATATAGAAAATGCTCATCAAGAGCAAACTGCTGTCACTCGGGAAGAAATTGACAAAAATGTAGATTATCAAGGATGGCTTGCAATAAAGAAAAGAAAGTGGAAAGATACTCTTGACAAGAGGAAGAAACAACGTGGAGCTTCCGAGCTCAGGGCAAGTTGCCAGTGCTGCTATGAGGCTGAGATACTGCCTTGGGGAGGGGCAGAACTCTGCATTGCCATCCAATTGTACAGATTGAATAATGAAAGGTCTGCTCATAATGCTAATGACAAGATGCTTGGTGGTGGGAGAAATCTCAAAGGGGCTCATGGGAAAATAGATATTACAACTAGTTCCGAGTTCGAGAGTGGCCAAATTTTGGCGTGGCTTGTTGTTGAAGGAACAATGCTTAAGATTCCTCTAAATGTTCCAAGAATTTTTTACATTAATTCTACATCACCTATAAAAGAAGAATTGAAAGAAGAATTACCAGGGAAGTATGTCAACAAGAGTCTTCCTCATGGAAGACATAGTTATTACCTATATGAGGTTTCAGTTGATGAGAAGAGGTTTAGGACATGGAGAAAAAAGCTTGCGGCTCTTATCGCTGATCCAGATATTGAGGTCGGTAGTTGTGAAGAAGGAGCTGATCTTCTTCGTGTTTTTGATGTGGGATTGATATGCTTTAGTCCCCAGCTTCGGAGCTTCGATGTTGTCTTGGGGCTTCGCGTGGTGGCCGTCGGCTGCGATCTAAGGGCAAGCAAAGGCACGGGCGGTAGCCTACTTGGCTGTGTTCCGGTAGGTCATTCCTTTAGTGGGAGTCCGCACCGCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

446

Amino Acids

50.32

Weight (kDa)

7.52

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
POL2_thumb PF22634 5 - 130 5.2e-36 DNA polymerase epsilon catalytic subunit A, thumb domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 51, 880
AccI GTMKAC 1 cut(s) 427
AccII CGCG 1 cut(s) 1230
AciI CCGC 8 cut(s) 13, 49, 146, 1097, 1275, 1325, 1330, 1336
AclWI GGATC 2 cut(s) 221, 1106
AcoI YGGCCR 2 cut(s) 871, 1236
AcsI RAATTY 3 cut(s) 472, 876, 936
AfaI GTAC 2 cut(s) 297, 752
AfiI CCNNNNNNNGG 7 cut(s) 51, 672, 719, 880, 1048, 1200, 1317
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 6 cut(s) 266, 386, 403, 760, 899, 979
AhlI ACTAGT 1 cut(s) 851
AjnI CCWGG 1 cut(s) 991
AluBI AGCT 6 cut(s) 662, 670, 1093, 1148, 1198, 1206
AluI AGCT 6 cut(s) 662, 670, 1093, 1148, 1198, 1206
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 2 cut(s) 319, 427
AlwI GGATC 2 cut(s) 221, 1106
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 2 cut(s) 871, 1236
ApeKI GCWGC 2 cut(s) 690, 1245
ApoI RAATTY 3 cut(s) 472, 876, 936
AseI ATTAAT 1 cut(s) 948
AspS9I GGNCC 2 cut(s) 348, 500
AsuHPI GGTGA 2 cut(s) 16, 951
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 2 cut(s) 348, 500
BalI TGGCCA 1 cut(s) 873
BanII GRGCYC 2 cut(s) 672, 829
BbsI GAAGAC 3 cut(s) 402, 1007, 1033
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 677, 1232
BccI CCATC 2 cut(s) 591, 749
BceAI ACGGC 1 cut(s) 1223
BciT130I CCWGG 1 cut(s) 993
BcoDI GTCTC 2 cut(s) 319, 427
BcuI ACTAGT 1 cut(s) 851
BfaI CTAG 2 cut(s) 368, 852
BfrI CTTAAG 1 cut(s) 911
BglI GCCNNNNNGGC 1 cut(s) 1287
BisI GCNGC 3 cut(s) 691, 1098, 1246
BlsI GCNGC 3 cut(s) 692, 1099, 1247
Bme1390I CCNGG 1 cut(s) 993
Bme18I GGWCC 2 cut(s) 348, 500
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 348, 500
BmrFI CCNGG 1 cut(s) 993
BmsI GCATC 3 cut(s) 199, 262, 786
BpiI GAAGAC 3 cut(s) 402, 1007, 1033
BplI GAGNNNNNCTC 2 cut(s) 1003, 1035
Bpu10I CCTNAGC 1 cut(s) 671
BsaI GGTCTC 2 cut(s) 319, 427
BsaJI CCNNGG 2 cut(s) 713, 992
BsaWI WCCGGW 1 cut(s) 1297
Bsc4I CCNNNNNNNGG 7 cut(s) 51, 672, 719, 880, 1048, 1200, 1317
Bse1I ACTGG 3 cut(s) 251, 350, 685
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 1 cut(s) 993
BseDI CCNNGG 2 cut(s) 713, 992
BseGI GGATG 2 cut(s) 602, 741
BseLI CCNNNNNNNGG 7 cut(s) 51, 672, 719, 880, 1048, 1200, 1317
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 685, 693
BseNI ACTGG 3 cut(s) 251, 350, 685
BseXI GCAGC 2 cut(s) 677, 1232
BseYI CCCAGC 1 cut(s) 1194
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 1230
BshFI GGCC 2 cut(s) 873, 1238
BsiHKAI GWGCWC 1 cut(s) 672
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 1 cut(s) 1298
BslFI GGGAC 1 cut(s) 1176
BslI CCNNNNNNNGG 7 cut(s) 51, 672, 719, 880, 1048, 1200, 1317
BsmAI GTCTC 2 cut(s) 319, 427
BsmFI GGGAC 1 cut(s) 1176
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 2 cut(s) 873, 1238
Bso31I GGTCTC 2 cut(s) 319, 427
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 672, 829
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
BspACI CCGC 8 cut(s) 13, 49, 146, 1097, 1275, 1325, 1330, 1336
BspANI GGCC 2 cut(s) 873, 1238
BspCNI CTCAG 2 cut(s) 684, 694
BspFNI CGCG 1 cut(s) 1230
BspPI GGATC 2 cut(s) 221, 1106
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 2 cut(s) 319, 427
BsrDI GCAATG 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 3 cut(s) 251, 350, 685
BssECI CCNNGG 2 cut(s) 713, 992
BssMI GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 1 cut(s) 993
Bst4CI ACNGT 2 cut(s) 95, 449
Bst6I CTCTTC 1 cut(s) 1061
BstAFI CTTAAG 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 750
BstC8I GCNNGC 6 cut(s) 53, 243, 603, 1095, 1261, 1334
BstDEI CTNAG 3 cut(s) 671, 702, 1253
BstENI CCTNNNNNAGG 2 cut(s) 717, 1046
BstF5I GGATG 2 cut(s) 602, 741
BstFNI CGCG 1 cut(s) 1230
BstKTI GATC 6 cut(s) 9, 61, 216, 1114, 1153, 1252
BstMAI GTCTC 2 cut(s) 319, 427
BstMBI GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
BstMWI GCNNNNNNNGC 7 cut(s) 41, 132, 690, 782, 1106, 1287, 1333
BstNI CCWGG 1 cut(s) 993
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 991
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 2 cut(s) 677, 1232
BstV2I GAAGAC 3 cut(s) 402, 1007, 1033
BsuRI GGCC 2 cut(s) 873, 1238
BtsCI GGATG 2 cut(s) 602, 741
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 5 cut(s) 100, 180, 241, 445, 692
Cac8I GCNNGC 6 cut(s) 53, 243, 603, 1095, 1261, 1334
CaiI CAGNNNCTG 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 348, 500
Csp6I GTAC 2 cut(s) 296, 751
CviAII CATG 6 cut(s) 130, 168, 230, 830, 1022, 1080
CviQI GTAC 2 cut(s) 296, 751
DdeI CTNAG 3 cut(s) 671, 702, 1253
DpnI GATC 6 cut(s) 8, 60, 215, 1113, 1152, 1251
DpnII GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
EaeI YGGCCR 2 cut(s) 871, 1236
Eam1104I CTCTTC 1 cut(s) 1061
EarI CTCTTC 1 cut(s) 1061
Ecl136II GAGCTC 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 713
Eco24I GRGCYC 2 cut(s) 672, 829
Eco31I GGTCTC 2 cut(s) 319, 427
Eco47I GGWCC 2 cut(s) 348, 500
Eco53kI GAGCTC 1 cut(s) 670
Eco88I CYCGRG 1 cut(s) 561
EcoICRI GAGCTC 1 cut(s) 670
EcoNI CCTNNNNNAGG 2 cut(s) 717, 1046
EcoRII CCWGG 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 2 cut(s) 672, 829
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 6 cut(s) 133, 171, 233, 833, 1025, 1083
FalI AAGNNNNNCTT 4 cut(s) 585, 617, 618, 650
FaqI GGGAC 1 cut(s) 1176
FatI CATG 6 cut(s) 129, 167, 229, 829, 1021, 1079
FauI CCCGC 1 cut(s) 6
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 691, 1098, 1246
FokI GGATG 2 cut(s) 609, 728
FriOI GRGCYC 2 cut(s) 672, 829
Fsp4HI GCNGC 3 cut(s) 691, 1098, 1246
FspBI CTAG 2 cut(s) 368, 852
GluI GCNGC 3 cut(s) 691, 1098, 1246
GsaI CCCAGC 1 cut(s) 1198
HaeIII GGCC 2 cut(s) 873, 1238
HapII CCGG 1 cut(s) 1298
Hin1II CATG 6 cut(s) 133, 171, 233, 833, 1025, 1083
HincII GTYRAC 2 cut(s) 325, 1006
HindII GTYRAC 2 cut(s) 325, 1006
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 4 cut(s) 119, 916, 1012, 1321
HpaII CCGG 1 cut(s) 1298
HphI GGTGA 2 cut(s) 16, 951
Hpy166II GTNNAC 4 cut(s) 325, 428, 445, 1006
Hpy188I TCNGA 5 cut(s) 118, 319, 667, 859, 1203
Hpy188III TCNNGA 9 cut(s) 65, 206, 368, 503, 542, 563, 635, 865, 1115
Hpy8I GTNNAC 4 cut(s) 325, 428, 445, 1006
Hpy99I CGWCG 1 cut(s) 1244
HpyAV CCTTC 2 cut(s) 893, 1136
HpyCH4III ACNGT 2 cut(s) 95, 449
HpyCH4IV ACGT 2 cut(s) 364, 655
HpyCH4V TGCA 7 cut(s) 173, 185, 257, 309, 408, 605, 735
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 132, 690, 782, 1106, 1287, 1333
HpyF3I CTNAG 3 cut(s) 671, 702, 1253
HpySE526I ACGT 2 cut(s) 364, 655
Hsp92II CATG 6 cut(s) 133, 171, 233, 833, 1025, 1083
Kzo9I GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
LmnI GCTCC 3 cut(s) 659, 1145, 1203
Lsp1109I GCAGC 2 cut(s) 677, 1232
LweI GCATC 3 cut(s) 199, 262, 786
MaeI CTAG 2 cut(s) 368, 852
MaeII ACGT 2 cut(s) 364, 655
MaeIII GTNAC 2 cut(s) 175, 556
MalI GATC 6 cut(s) 8, 60, 215, 1113, 1152, 1251
MboI GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
MfeI CAATTG 2 cut(s) 438, 746
MhlI GDGCHC 2 cut(s) 672, 829
MlsI TGGCCA 1 cut(s) 873
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 2 cut(s) 1021, 1330
MnlI CCTC 9 cut(s) 491, 636, 691, 713, 930, 1029, 1042, 1062, 1117
Mox20I TGGCCA 1 cut(s) 873
MscI TGGCCA 1 cut(s) 873
MseI TTAA 3 cut(s) 459, 912, 948
MslI CAYNNNNRTG 2 cut(s) 234, 450
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 2 cut(s) 51, 1332
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 1 cut(s) 1298
MspR9I CCNGG 1 cut(s) 993
MunI CAATTG 2 cut(s) 438, 746
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 993
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 7 cut(s) 41, 132, 690, 782, 1106, 1287, 1333
NdeII GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
NlaIII CATG 6 cut(s) 133, 171, 233, 833, 1025, 1083
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 233
PciI ACATGT 1 cut(s) 229
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 119, 916
PflMI CCANNNNNTGG 2 cut(s) 51, 880
PkrI GCNGC 3 cut(s) 692, 1099, 1247
PleI GAGTC 2 cut(s) 1020, 1329
PpsI GAGTC 2 cut(s) 1020, 1329
PscI ACATGT 1 cut(s) 229
PshBI ATTAAT 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 672
Psp6I CCWGG 1 cut(s) 991
PspFI CCCAGC 1 cut(s) 1194
PspGI CCWGG 1 cut(s) 991
PspPI GGNCC 2 cut(s) 348, 500
PstNI CAGNNNCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 297, 752
RsaNI GTAC 2 cut(s) 296, 751
RseI CAYNNNNRTG 2 cut(s) 234, 450
SacI GAGCTC 1 cut(s) 672
SaqAI TTAA 3 cut(s) 459, 912, 948
SatI GCNGC 3 cut(s) 691, 1098, 1246
Sau3AI GATC 6 cut(s) 6, 58, 213, 1111, 1150, 1249
Sau96I GGNCC 2 cut(s) 348, 500
SchI GAGTC 2 cut(s) 1021, 1330
ScrFI CCNGG 1 cut(s) 993
SduI GDGCHC 2 cut(s) 672, 829
SfaNI GCATC 3 cut(s) 199, 262, 786
SinI GGWCC 2 cut(s) 348, 500
SmiMI CAYNNNNRTG 2 cut(s) 234, 450
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
SpeI ACTAGT 1 cut(s) 851
SsiI CCGC 8 cut(s) 13, 49, 146, 1097, 1275, 1325, 1330, 1336
SspI AATATT 1 cut(s) 261
SspMI CTAG 2 cut(s) 368, 852
SstI GAGCTC 1 cut(s) 672
StyD4I CCNGG 1 cut(s) 991
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 2 cut(s) 95, 449
TaiI ACGT 2 cut(s) 367, 658
TaqI TCGA 2 cut(s) 864, 1209
TaqII GACCGA 1 cut(s) 445
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 2 cut(s) 119, 916
Tru1I TTAA 3 cut(s) 459, 912, 948
Tru9I TTAA 3 cut(s) 459, 912, 948
TscAI CASTG 5 cut(s) 100, 187, 241, 452, 692
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 2 cut(s) 690, 1245
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 299, 387, 468, 780
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 5 cut(s) 100, 187, 241, 452, 692
Van91I CCANNNNNTGG 2 cut(s) 51, 880
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 2 cut(s) 348, 500
VspI ATTAAT 1 cut(s) 948
XagI CCTNNNNNAGG 2 cut(s) 717, 1046
XapI RAATTY 3 cut(s) 472, 876, 936
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 427
XspI CTAG 2 cut(s) 368, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.