Rw5G033580

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001971
Physical Location & Seq
Forward (+)
57196645 .. 57205304
8660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033580.1

Sequence Viewer

Length: 1428 bp
ATGAGCGGCTACGAGAGCCGGAAACGGGACCGGAGAGACTCGTGGAGCTCCAAGAAGCAGCAGAAGCTCACGCGCACCGCCGACGAAGACCTAGAGTCAAAGCTCGGATTCGATCTATTCTCCGAAGGTGAAACTAGGCTTGGCTGGTTACTCACTTTCGCAACCTCATCCTGTGAAGATCAAGACACCCATAAAGTTTATAGCTGTGTTGATCTGTATTTCGTTACCCAGGATGGCTCTGCTTTCAAGTCAAAATACAAGTTTCGCCCTTATTTCTATGCAGCCACAAAGGAAAAAATGGAAATGGATGTTGAAGCATATTTGAGACGGCGGTATGAAAGTCGTATTGCTGATATCGAAATAGTGGCGAAAGAAGATCTTGATCTTAAAAACCATTTGTCTGGCCTACACAAGTCCTATTTGAAGTTATCATTTGATACTGTTCAACAATTAATGAATGTCAAGAGTGACCTATTGCAAGTCGTCGAAAGAAACCACACAAAATTTGATGCTGCCGAAGCTTATGAGTCAATACTGCTAGGGAAACGGGAACAAAGGCCTCAAGATTTCCTAGATTGTATTGTTGATCTTCGTGAATACGATGTTCCCTACCATGTTCGCTTCGCTATTGACAATGATGTGCGATGTGGCCAGTGGTACAATGTTAGTGTATCCAGTACGGGTGTTATGCTTGAGAGGAGGACAGATCTTCTGCAGCATGCTGAAGTTCATGTTTGTGCATTTGATATTGAGACAACTAAGCTGCCCTTAAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGACGGACAAGGATACCTGATTATTAATAGAGAGTGTGTTGGAGCTGATATAGAGTATTTGGAGTACACTCCAAAAGAAGAATTTGTAGGGCATTTCAAGGTGACAAATGTGGAAAATGAGAGAGAGCTTCTAAGACAGTGGTTTGCCCATATGCGGGAGGTGAAGCCTGGAATCTATGTCACATACAATGCATTAGGTTCAGCGCATTCGGTGGTCATAGGGCTAGATAGTCTTTATATGGAGACTGATATATGTAAGAATGTGCTCCCCAGTCCTCGATCCACTACCACTTTCCACATCTACGACGGCAATGTTGACCACCATCGCCACCACCGCGAAATACGACGTCGTTTCGCCACCCTCAAGCTCGGCGACTTGTTCAACAAGCTATTCCTCATGTTCTTCATCTTCATCCCTCTCGTTGTCCTCATCCTCTTTTCACCACCGACATCAAGAGCCTCTTCTTCTCCCACCTCTCCGTCCCTCTCTCCGATTCACTCTCTGATAAGCTCCACAAGTCCGAATTACAAGTTCTCTTCTTGCTCCTCCAGCAACAGCTCGGTCTCTTCGGCATCAGGAACCAAACGCATCCCAATTCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

475

Amino Acids

54.79

Weight (kDa)

6.26

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 196 - 337 1.1e-38 DNA polymerase family B, exonuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1171
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 2 cut(s) 73, 1158
AciI CCGC 5 cut(s) 6, 78, 331, 976, 1156
AclWI GGATC 1 cut(s) 1095
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 3 cut(s) 503, 773, 902
AcuI CTGAAG 1 cut(s) 744
AcyI GRCGYC 1 cut(s) 1168
AfaI GTAC 3 cut(s) 659, 679, 887
AfiI CCNNNNNNNGG 3 cut(s) 25, 975, 976
AgsI TTSAA 6 cut(s) 247, 314, 424, 446, 919, 1204
AhdI GACNNNNNGTC 1 cut(s) 94
AjnI CCWGG 2 cut(s) 228, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
Alw21I GWGCWC 2 cut(s) 50, 1089
Alw26I GTCTC 5 cut(s) 30, 319, 746, 1058, 1390
AlwI GGATC 1 cut(s) 1095
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 3 cut(s) 403, 557, 649
ApeKI GCWGC 5 cut(s) 58, 281, 512, 715, 763
ApoI RAATTY 3 cut(s) 503, 773, 902
AseI ATTAAT 2 cut(s) 452, 846
AspLEI GCGC 2 cut(s) 75, 1027
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 4 cut(s) 140, 934, 994, 1254
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 651
BanII GRGCYC 1 cut(s) 50
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 50, 1089
BbvI GCAGC 5 cut(s) 70, 293, 499, 727, 750
BccI CCATC 2 cut(s) 227, 1152
BceAI ACGGC 2 cut(s) 344, 1144
BciT130I CCWGG 2 cut(s) 230, 990
BciVI GTATCC 2 cut(s) 682, 827
BcoDI GTCTC 5 cut(s) 30, 319, 746, 1058, 1390
BfaI CTAG 5 cut(s) 92, 135, 539, 572, 1046
BfmI CTRYAG 1 cut(s) 713
BfuI GTATCC 2 cut(s) 682, 827
BglII AGATCT 2 cut(s) 376, 706
BisI GCNGC 6 cut(s) 7, 59, 282, 513, 716, 764
BlsI GCNGC 6 cut(s) 8, 60, 283, 514, 717, 765
Bme1390I CCNGG 2 cut(s) 230, 990
Bme18I GGWCC 1 cut(s) 28
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 29, 1402
BmrFI CCNGG 2 cut(s) 230, 990
BmrI ACTGGG 1 cut(s) 1086
BmsI GCATC 4 cut(s) 499, 772, 1403, 1419
BmuI ACTGGG 1 cut(s) 1086
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 1354
BpuEI CTTGAG 3 cut(s) 546, 713, 1169
BsaBI GATNNNNATC 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 1168
BsaI GGTCTC 1 cut(s) 1390
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 975, 976
Bse1I ACTGG 3 cut(s) 652, 675, 1092
Bse3DI GCAATG 1 cut(s) 1138
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 2 cut(s) 230, 990
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 6 cut(s) 167, 238, 313, 1233, 1251, 1410
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 3 cut(s) 25, 975, 976
BseMI GCAATG 1 cut(s) 1138
BseNI ACTGG 3 cut(s) 652, 675, 1092
BseRI GAGGAG 2 cut(s) 712, 1357
BseXI GCAGC 5 cut(s) 70, 293, 499, 727, 750
Bsh1236I CGCG 2 cut(s) 73, 1158
BshFI GGCC 3 cut(s) 405, 559, 651
BsiHKAI GWGCWC 2 cut(s) 50, 1089
BsiSI CCGG 2 cut(s) 19, 31
BslFI GGGAC 2 cut(s) 41, 1288
BslI CCNNNNNNNGG 3 cut(s) 25, 975, 976
BsmAI GTCTC 5 cut(s) 30, 319, 746, 1058, 1390
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 41, 1288
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 3 cut(s) 405, 559, 651
Bso31I GGTCTC 1 cut(s) 1390
Bsp1286I GDGCHC 2 cut(s) 50, 1089
Bsp143I GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
BspACI CCGC 5 cut(s) 6, 78, 331, 976, 1156
BspANI GGCC 3 cut(s) 405, 559, 651
BspFNI CGCG 2 cut(s) 73, 1158
BspLI GGNNCC 2 cut(s) 29, 1402
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 1 cut(s) 1095
BspTNI GGTCTC 1 cut(s) 1390
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 1138
BsrI ACTGG 3 cut(s) 652, 675, 1092
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
BssNI GRCGYC 1 cut(s) 1168
BssSI CACGAG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 230, 990
Bst4CI ACNGT 2 cut(s) 442, 960
Bst6I CTCTTC 3 cut(s) 1288, 1363, 1393
BstACI GRCGYC 1 cut(s) 1168
BstC8I GCNNGC 1 cut(s) 720
BstDEI CTNAG 2 cut(s) 759, 953
BstF5I GGATG 6 cut(s) 167, 238, 313, 1233, 1251, 1410
BstFNI CGCG 2 cut(s) 73, 1158
BstHHI GCGC 2 cut(s) 75, 1027
BstKTI GATC 8 cut(s) 115, 181, 214, 379, 385, 589, 709, 1103
BstMAI GTCTC 5 cut(s) 30, 319, 746, 1058, 1390
BstMBI GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
BstMWI GCNNNNNNNGC 5 cut(s) 15, 64, 518, 1155, 1371
BstNI CCWGG 2 cut(s) 230, 990
BstNSI RCATGY 1 cut(s) 722
BstSCI CCNGG 2 cut(s) 228, 988
BstSFI CTRYAG 1 cut(s) 713
BstUI CGCG 2 cut(s) 73, 1158
BstV1I GCAGC 5 cut(s) 70, 293, 499, 727, 750
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 376, 706
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 2 cut(s) 376, 706
BsuI GTATCC 2 cut(s) 682, 827
BsuRI GGCC 3 cut(s) 405, 559, 651
BtgZI GCGATG 2 cut(s) 658, 1130
BtsCI GGATG 6 cut(s) 167, 238, 313, 1233, 1251, 1410
BtsIMutI CAGTG 2 cut(s) 659, 965
Cac8I GCNNGC 1 cut(s) 720
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 2 cut(s) 75, 1027
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 658, 678, 886
CviAII CATG 5 cut(s) 614, 719, 731, 817, 1219
CviQI GTAC 3 cut(s) 658, 678, 886
DdeI CTNAG 2 cut(s) 759, 953
DpnI GATC 8 cut(s) 114, 180, 213, 378, 384, 588, 708, 1102
DpnII GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
DriI GACNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 3 cut(s) 1288, 1363, 1393
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 3 cut(s) 1288, 1363, 1393
Ecl136II GAGCTC 1 cut(s) 48
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 1390
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 744
EcoICRI GAGCTC 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 228, 988
EcoRV GATATC 1 cut(s) 355
EcoT22I ATGCAT 1 cut(s) 1015
EcoT38I GRGCYC 1 cut(s) 50
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 5 cut(s) 617, 722, 734, 820, 1222
FalI AAGNNNNNCTT 6 cut(s) 363, 395, 752, 784, 1267, 1299
FaqI GGGAC 2 cut(s) 41, 1288
FatI CATG 5 cut(s) 613, 718, 730, 816, 1218
FauI CCCGC 1 cut(s) 969
FauNDI CATATG 1 cut(s) 972
Fnu4HI GCNGC 6 cut(s) 7, 59, 282, 513, 716, 764
FokI GGATG 6 cut(s) 154, 245, 320, 1220, 1238, 1397
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 6 cut(s) 7, 59, 282, 513, 716, 764
FspBI CTAG 5 cut(s) 92, 135, 539, 572, 1046
GlaI GCGC 2 cut(s) 74, 1026
GluI GCNGC 6 cut(s) 7, 59, 282, 513, 716, 764
GsuI CTGGAG 1 cut(s) 1354
HaeIII GGCC 3 cut(s) 405, 559, 651
HapII CCGG 2 cut(s) 19, 31
HhaI GCGC 2 cut(s) 75, 1027
Hin1I GRCGYC 1 cut(s) 1168
Hin1II CATG 5 cut(s) 617, 722, 734, 820, 1222
Hin6I GCGC 2 cut(s) 73, 1025
HinP1I GCGC 2 cut(s) 73, 1025
HincII GTYRAC 2 cut(s) 823, 1138
HindII GTYRAC 2 cut(s) 823, 1138
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 6 cut(s) 38, 95, 108, 527, 993, 1315
HpaII CCGG 2 cut(s) 19, 31
HphI GGTGA 4 cut(s) 140, 934, 994, 1254
Hpy166II GTNNAC 3 cut(s) 823, 888, 1138
Hpy188I TCNGA 5 cut(s) 107, 124, 1314, 1326, 1344
Hpy188III TCNNGA 8 cut(s) 182, 380, 463, 563, 593, 779, 1275, 1398
Hpy8I GTNNAC 3 cut(s) 823, 888, 1138
Hpy99I CGWCG 5 cut(s) 86, 488, 1130, 1170, 1173
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 2 cut(s) 442, 960
HpyCH4IV ACGT 1 cut(s) 1168
HpyCH4V TGCA 5 cut(s) 281, 478, 715, 740, 1013
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 64, 518, 1155, 1371
HpyF3I CTNAG 2 cut(s) 759, 953
HpySE526I ACGT 1 cut(s) 1168
Hsp92I GRCGYC 1 cut(s) 1168
Hsp92II CATG 5 cut(s) 617, 722, 734, 820, 1222
HspAI GCGC 2 cut(s) 73, 1025
Kzo9I GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
LmnI GCTCC 6 cut(s) 45, 53, 863, 1092, 1337, 1370
Lsp1109I GCAGC 5 cut(s) 70, 293, 499, 727, 750
LweI GCATC 4 cut(s) 499, 772, 1403, 1419
MaeI CTAG 5 cut(s) 92, 135, 539, 572, 1046
MaeII ACGT 1 cut(s) 1168
MaeIII GTNAC 5 cut(s) 147, 223, 467, 922, 1000
MalI GATC 8 cut(s) 114, 180, 213, 378, 384, 588, 708, 1102
MbiI CCGCTC 1 cut(s) 6
MboI GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
MflI RGATCY 2 cut(s) 376, 706
MhlI GDGCHC 2 cut(s) 50, 1089
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 6 cut(s) 449, 503, 773, 902, 1345, 1416
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 3 cut(s) 32, 104, 536
MmeI TCCRAC 1 cut(s) 841
Mox20I TGGCCA 1 cut(s) 651
Mph1103I ATGCAT 1 cut(s) 1015
MscI TGGCCA 1 cut(s) 651
MseI TTAA 5 cut(s) 387, 452, 770, 797, 846
MslI CAYNNNNRTG 1 cut(s) 735
Msp20I TGGCCA 1 cut(s) 651
MspI CCGG 2 cut(s) 19, 31
MspR9I CCNGG 2 cut(s) 230, 990
Mva1269I GAATGC 1 cut(s) 1027
MvaI CCWGG 2 cut(s) 230, 990
MvnI CGCG 2 cut(s) 73, 1158
MwoI GCNNNNNNNGC 5 cut(s) 15, 64, 518, 1155, 1371
NdeI CATATG 1 cut(s) 972
NdeII GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
NlaIII CATG 5 cut(s) 617, 722, 734, 820, 1222
NlaIV GGNNCC 2 cut(s) 29, 1402
NmeAIII GCCGAG 1 cut(s) 1170
NmuCI GTSAC 3 cut(s) 467, 922, 1000
NsiI ATGCAT 1 cut(s) 1015
NspI RCATGY 1 cut(s) 722
PaeI GCATGC 1 cut(s) 722
PceI AGGCCT 1 cut(s) 559
PctI GAATGC 1 cut(s) 1027
PfeI GAWTC 3 cut(s) 108, 993, 1315
PkrI GCNGC 6 cut(s) 8, 60, 283, 514, 717, 765
PleI GAGTC 3 cut(s) 32, 103, 535
PpsI GAGTC 3 cut(s) 32, 103, 535
PshBI ATTAAT 2 cut(s) 452, 846
Psp124BI GAGCTC 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 228, 988
PspGI CCWGG 2 cut(s) 228, 988
PspN4I GGNNCC 2 cut(s) 29, 1402
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 2 cut(s) 376, 706
RsaI GTAC 3 cut(s) 659, 679, 887
RsaNI GTAC 3 cut(s) 658, 678, 886
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 1 cut(s) 50
SaqAI TTAA 5 cut(s) 387, 452, 770, 797, 846
SatI GCNGC 6 cut(s) 7, 59, 282, 513, 716, 764
Sau3AI GATC 8 cut(s) 112, 178, 211, 376, 382, 586, 706, 1100
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 3 cut(s) 32, 104, 536
ScrFI CCNGG 2 cut(s) 230, 990
SduI GDGCHC 2 cut(s) 50, 1089
SfaNI GCATC 4 cut(s) 499, 772, 1403, 1419
SfcI CTRYAG 1 cut(s) 713
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 3 cut(s) 561, 692, 1184
SmoI CTYRAG 3 cut(s) 561, 692, 1184
SphI GCATGC 1 cut(s) 722
Sse9I AATT 6 cut(s) 449, 503, 773, 902, 1345, 1416
SseBI AGGCCT 1 cut(s) 559
SsiI CCGC 5 cut(s) 6, 78, 331, 976, 1156
SspMI CTAG 5 cut(s) 92, 135, 539, 572, 1046
SstI GAGCTC 1 cut(s) 50
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 2 cut(s) 228, 988
TaaI ACNGT 2 cut(s) 442, 960
TaiI ACGT 1 cut(s) 1171
TaqI TCGA 4 cut(s) 111, 357, 486, 1099
TaqII GACCGA 1 cut(s) 1372
TasI AATT 6 cut(s) 449, 503, 773, 902, 1345, 1416
TatI WGTACW 1 cut(s) 885
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 3 cut(s) 108, 993, 1315
Tru1I TTAA 5 cut(s) 387, 452, 770, 797, 846
Tru9I TTAA 5 cut(s) 387, 452, 770, 797, 846
TscAI CASTG 2 cut(s) 659, 965
TseFI GTSAC 3 cut(s) 467, 922, 1000
TseI GCWGC 5 cut(s) 58, 281, 512, 715, 763
Tsp45I GTSAC 3 cut(s) 467, 922, 1000
TspDTI ATGAA 6 cut(s) 351, 470, 719, 801, 1216, 1222
TspGWI ACGGA 2 cut(s) 840, 1290
TspRI CASTG 2 cut(s) 659, 965
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 2 cut(s) 452, 846
XapI RAATTY 3 cut(s) 503, 773, 902
XceI RCATGY 1 cut(s) 722
XspI CTAG 5 cut(s) 92, 135, 539, 572, 1046
ZraI GACGTC 1 cut(s) 1169
Zsp2I ATGCAT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.