Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

10467956 .. 10472211

Gene Structure 4256 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G29900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3564 bp

ATGAGAAACCATTGCTTAGAACTCTCTTCCAATTGTTCCTCCATTTTCGCTTCTTCCAAATCCAATCCTCGTTTCTCTCCTTCCAAGCTCTCCTATTCCACTTTCTTCTCTCGCTCTGCCATCTATTACAGATCAAAACCAAAACAAGCCTCGTCTTCTTCTTCCTTCTCCACTTTCCCCCCATGTCTCAATCGGAAAAGCTCCCTCACGCATGTTCTCAAACCCGTCTCAGAGCTCGCCGACACCACTACCAAGCCTTTTTCTCCGGAGATCGTCGGCAAGAGAACCGATCTGAAGAAGATTATGATTCTCGGCGCTGGTCCGATTGTCATTGGACAAGCTTGTGAGTTTGATTACTCTGGTACTCAAGCTTGTAAAGCCTTAAGAGAAGAGGGCTATGAGGTTATCCTGATCAATTCGAATCCTGCCACTATCATGACTGATCCGGAAACTGCTAATCGGACTTATATCGCTCCGATGACTCCTGAGCTTGTCGAGCAGGTTATTGAGAAAGAGAGGCCTGACGCTTTGTTACCAACCATGGGTGGTCAAACCGCATTGAACCTCGCGGTTGCTCTTGCTGAGAGTGGTGCTTTGGAGAAATACGGTGTTGAATTGATAGGAGCTAAGCTTGGTGCGATTAAGAAAGCTGAAGATCGTGAGTTGTTCAAGGATGCGATGAAGAACATTGGGCTAAAGACTCCACCTTCAGGGATTGGGACCACTCTTGATGAGTGTTTTGACATTGCTGAGAAAATTGGTGAGTTCCCTTTGATTATCCGTCCTGCGTTTACTTTAGGTGGTACTGGTGGTGGAATTGCGTATAACAAAGAGGAGTTTGAGTCTATATGTAAATCGGGTTTGGCTGCGAGTGCGACAAGTCAAGTTCTTGTGGAGAAATCCTTGTTGGGTTGGAAAGAATATGAGCTTGAGGTGATGAGAGACTTAGCTGACAATGTTGTCATTATCTGTTCCATTGAGAATATTGATCCTATGGGTGTGCACACTGGTGATTCCATCACTGTGGCACCTGCACAGACTCTAACGGATAGAGAGTACCAGCGGCTTAGGGATTATTCCATTGCGATTATACGGGAGATTGGTGTTGAGTGTGGTGGATCTAATGTGCAGTTTGCTGTCAACCCGGTTGATGGTGAAGTTATGATCATAGAGATGAACCCTAGGGTCTCAAGATCTTCTGCTCTTGCTTCCAAGGCTACAGGGTTTCCCATTGCTAAAATGGCTGCCAAGTTGTCTGTTGGCTATACCTTGGATCAGATTCCTAATGATATCACGAGGAAAACACCGGCTAGCTTCGAGCCCTCCATCGATTATGTGGTGACTAAGATTCCTCGATTTGCATTTGAAAAGTTTCCAGGATCTCAGCCATTGCTAACGACCCAGATGAAATCTGTTGGGGAATCTATGGCTCTCGGCCGTACATTCCAAGAATCTTTCCAGAAAGCTCTGAGGTCTCTGGAGTGTGGATTCTCGGGTTGGGGTTGTGCAAAAATTAAAGAGCTAGATTGGGACTGGGATCAGCTGAAATACAGCCTAAGAGTCCCAAATCCTGACAGGATCCATGCGATATATGCTGCCATGAAAAAGGGTATGAAAATTGATGAAATCTACGAGTTGAGCATGGTGGACAAGTGGTTCCTAACCCAGCTTAAAGAGCTCGTGGACGTCGAACAGTATCTTATGTCCGGAACCTTGTCAGAGATTACAAAAGAAGACCTTTACGAAGTCAAAAAGCGGGGATTTAGTGACAAGCAAATCGCTTTTGCTACAAAGACAACCGAGGAAGAAGTCCGTACCAAGCGGATTTCTCTAGGAGTTGTTCCATCTTACAAGAGAGTGGATACATGTGCTGCAGAGTTCGAAGCGCATACACCATACATGTACTCTTCATATGATGTTGAATGTGAATCAGCTCCAAACAACAAGAAGAAGGTTTTGATTTTGGGTGGAGGGCCAAACCGCATTGGTCAAGGGATTGAATTTGATTACTGTTGTTGCCACACATCTTTCGCCTTACAGGATGCTGGATATGAGACCATAATGTTGAACTCAAATCCTGAAACAGTATCCACAGATTATGATACAAGCGATAGGCTCTATTTTGAACCTCTCACAATCGAGGATGTTCTCAATGTTATCGACCTTGAGAAACCTGATGGCATAATAGTGCAATTTGGTGGTCAAACTCCTCTGAAACTTGCTCTGCCGATCAAACATTATTTGGATAAGCACATGCCCATGAGCTTGAGCGGAGCGGGACCTGTTCGCATCTGGGGTACATCACCTGACTCCATTGACGCTGCTGAAGACAGAGAGAGGTTCAATGCAATTCTCGACGAGCTGAAGATTGAGCAGCCCAAGGGAGGCATTGCAAAGAGCGAAGCTGATGCATTAGCCATAGCAAAGGAGGTAGGGTACCCAGTTGTGGTAAGACCTTCTTATGTTCTAGGTGGACGAGCAATGGAGATCGTTTATGATGACAGTAGACTAATAACCTATTTGGAAAATGCGGTACAAGTTGACCCAGAGAGACCTGTTTTGGTAGATAAATATCTTTCTGATGCCATTGAGATCGACGTTGATACCCTTACTGATTCCTATGGAAATGTGGTGATTGGTGGAATAATGGAGCATATCGAACAAGCTGGTGTGCATTCTGGTGACTCAGCTTGTATGCTTCCAACACAAACCATCCCAGCTTCTTGTTTGCAAACTATTCGAACATGGACCACTAAGCTGGCGAAGAAGCTAAATGTATGTGGGCTGATGAACTGTCAGTACGCAATCACAACATCTGGGGATGTTTTTTTGCTGGAAGCCAATCCCCGAGCTTCCCGTACTGTCCCTTTTGTGTCAAAAGCCATTGGACACCCTCTTGCCAAGTATGCAGCGCTGGTCATGTCGGGCAAATCTCTCAAAGATCTTAACTTTGAAAAAGAAGTTATCCCTAAACATGTCTCTGTGAAAGAAGCTGTTTTCCCGTTTGAGAAGTTCCAAGGATGCGATGTGATACTCGGGCCAGAGATGAGAAGCACAGGAGAAGTGATGAGCATCAGTTCTGAATTCTCAAGTGCGTTTGCAATGGCTCAGATCGCTGCAGGTCAAAAGCTACCTCTATCAGGCACAGTCTTCCTCAGCTTAAACGATATGACCAAACCGCACCTGGAGAAAATCGCGGTGTCCTTCCTCGAGCTTGGGTTCAAAATAGTTGCCACCTCGGGAACAGCTCATTTCCTGGAACTGAAAGGCATTCCAGTGGAGAGAGTGTTGAAGTTGCATGAAGGAAGACCACATGCTGCTGATATGGTGGCGAATGGTCAGATCCATTTGATGTTGATCACAAGCTCGGGTGATGCTCTTGATCAGAAAGATGGGAGACAGCTCAGACAAATGGCTCTAGCATACAAGGTACCTGTTATAACCACTGTTGCTGGTGCATTGGCCACTGCTGAGGGAATCAAGAGCTTGAAGTCAAGTGCCATTAAAATGACCGCTCTTCAGGACTTCTTTGAGGTAAAGAATGTATCTTCTTTGCTCGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1187

Amino Acids

129.96

Weight (kDa)

5.53

Isoelectric Point (pI)

38.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	98 - 215	3.6e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	219 - 426	9.3e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	518 - 596	1.6e-26	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	649 - 773	6e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	777 - 980	3.5e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	785 - 951	7.1e-07	ATP-grasp domain
Dala_Dala_lig_C	PF07478	804 - 949	2.3e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1062 - 1148	6.2e-20	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3440
AarI	CACCTGC	1 cut(s)	1039
AasI	GACNNNNNNGTC	1 cut(s)	959
AatII	GACGTC	1 cut(s)	1689
Acc36I	ACCTGC	3 cut(s)	490, 1039, 3110
Acc65I	GGTACC	2 cut(s)	2436, 3430
AccB1I	GGYRCC	3 cut(s)	1027, 2436, 3430
AccBSI	CCGCTC	3 cut(s)	2271, 2276, 3515
AccI	GTMKAC	1 cut(s)	2506
AccII	CGCG	2 cut(s)	569, 3197
AccIII	TCCGGA	3 cut(s)	265, 445, 1706
AclWI	GGATC	9 cut(s)	437, 983, 1126, 1281, 1387, 1545, 1573, 1586, 3337
AcoI	YGGCCR	2 cut(s)	1435, 3462
AcsI	RAATTY	2 cut(s)	2000, 3083
AcuI	CTGAAG	6 cut(s)	314, 672, 693, 2346, 2384, 3503
AcyI	GRCGYC	1 cut(s)	1686
AfeI	AGCGCT	1 cut(s)	2913
AfiI	CCNNNNNNNGG	6 cut(s)	542, 710, 1151, 2384, 2446, 3140
AflII	CTTAAG	1 cut(s)	382
AflIII	ACRYGT	3 cut(s)	1865, 1899, 2974
AjnI	CCWGG	3 cut(s)	1375, 3183, 3255
AjuI	GAANNNNNNNTTGG	4 cut(s)	56, 88, 890, 922
AleI	CACNNNNGTG	1 cut(s)	1008
AloI	GAACNNNNNNTCC	2 cut(s)	1640, 1672
Alw21I	GWGCWC	3 cut(s)	237, 1005, 1680
Alw26I	GTCTC	9 cut(s)	191, 232, 936, 1192, 1479, 2048, 2545, 2983, 3391
Alw44I	GTGCAC	1 cut(s)	1001
AlwI	GGATC	9 cut(s)	437, 983, 1126, 1281, 1387, 1545, 1573, 1586, 3337
Ama87I	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
Aor13HI	TCCGGA	3 cut(s)	265, 445, 1706
Aor51HI	AGCGCT	1 cut(s)	2913
AoxI	GGCC	5 cut(s)	518, 1435, 1973, 3038, 3462
ApaLI	GTGCAC	1 cut(s)	1001
ApeKI	GCWGC	9 cut(s)	866, 1244, 1595, 1871, 2321, 2374, 2909, 3116, 3317
ApoI	RAATTY	2 cut(s)	2000, 3083
Asp700I	GAANNNNTTC	1 cut(s)	1371
Asp718I	GGTACC	2 cut(s)	2436, 3430
AspA2I	CCTAGG	1 cut(s)	1181
AspLEI	GCGC	3 cut(s)	317, 1888, 2914
AspS9I	GGNCC	6 cut(s)	320, 720, 1973, 2279, 2748, 3038
AsuC2I	CCSGG	1 cut(s)	1145
AsuHPI	GGTGA	9 cut(s)	773, 946, 1022, 1166, 1351, 2295, 2644, 2693, 3383
AsuII	TTCGAA	3 cut(s)	419, 1881, 2740
AsuNHI	GCTAGC	1 cut(s)	1310
AvaI	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
AvaII	GGWCC	4 cut(s)	320, 720, 2279, 2748
AvrII	CCTAGG	1 cut(s)	1181
BaeGI	GKGCMC	1 cut(s)	1005
BalI	TGGCCA	1 cut(s)	3464
BamHI	GGATCC	1 cut(s)	1578
BanI	GGYRCC	3 cut(s)	1027, 2436, 3430
BanII	GRGCYC	3 cut(s)	237, 1323, 1680
BauI	CACGAG	2 cut(s)	1294, 1679
BbsI	GAAGAC	5 cut(s)	147, 1740, 2334, 3142, 3313
Bbv12I	GWGCWC	3 cut(s)	237, 1005, 1680
BbvCI	CCTCAGC	2 cut(s)	3155, 3471
BbvI	GCAGC	9 cut(s)	853, 1231, 1582, 1858, 2308, 2386, 2921, 3103, 3304
BccI	CCATC	8 cut(s)	128, 1025, 1145, 1334, 1852, 2171, 2720, 3386
BceAI	ACGGC	1 cut(s)	1422
BcgI	CGANNNNNNTGC	4 cut(s)	2390, 2424, 2720, 2754
BciT130I	CCWGG	3 cut(s)	1377, 3185, 3257
BciVI	GTATCC	2 cut(s)	1855, 2098
BclI	TGATCA	4 cut(s)	411, 1164, 3357, 3382
BcnI	CCSGG	1 cut(s)	1145
BcoDI	GTCTC	9 cut(s)	191, 232, 936, 1192, 1479, 2048, 2545, 2983, 3391
BfaI	CTAG	6 cut(s)	1182, 1311, 1523, 1832, 2468, 3419
BfmI	CTRYAG	3 cut(s)	1218, 1872, 3117
BfoI	RGCGCY	2 cut(s)	318, 2915
BfrI	CTTAAG	1 cut(s)	382
BfuAI	ACCTGC	3 cut(s)	490, 1039, 3110
BfuI	GTATCC	2 cut(s)	1855, 2098
BglII	AGATCT	2 cut(s)	1193, 2941
BlnI	CCTAGG	1 cut(s)	1181
BlpI	GCTNAGC	1 cut(s)	627
Bme1390I	CCNGG	4 cut(s)	1145, 1377, 3185, 3257
Bme18I	GGWCC	4 cut(s)	320, 720, 2279, 2748
BmeT110I	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
BmgT120I	GGNCC	6 cut(s)	320, 720, 1973, 2279, 2748, 3038
BmiI	GGNNCC	8 cut(s)	721, 1029, 1580, 1658, 1711, 2280, 2438, 3432
BmrFI	CCNGG	4 cut(s)	1145, 1377, 3185, 3257
BmrI	ACTGGG	2 cut(s)	1543, 2435
BmsI	GCATC	8 cut(s)	664, 2032, 2298, 2398, 2572, 3011, 3081, 3364
BmtI	GCTAGC	1 cut(s)	1314
BmuI	ACTGGG	2 cut(s)	1543, 2435
BpiI	GAAGAC	5 cut(s)	147, 1740, 2334, 3142, 3313
BpmI	CTGGAG	2 cut(s)	1499, 3206
Bpu10I	CCTNAGC	4 cut(s)	486, 1067, 3155, 3471
Bpu1102I	GCTNAGC	1 cut(s)	627
Bpu14I	TTCGAA	3 cut(s)	419, 1881, 2740
BpuEI	CTTGAG	6 cut(s)	351, 950, 1174, 2186, 2287, 3073
BpuMI	CCSGG	1 cut(s)	1145
Bsa29I	ATCGAT	1 cut(s)	1329
BsaBI	GATNNNNATC	3 cut(s)	2571, 3071, 3356
BsaHI	GRCGYC	1 cut(s)	1686
BsaI	GGTCTC	4 cut(s)	1192, 1479, 2048, 2545
BsaJI	CCNNGG	8 cut(s)	540, 1181, 1212, 1269, 1800, 2379, 3016, 3237
BsaWI	WCCGGW	3 cut(s)	265, 445, 1706
BsaXI	ACNNNNNCTCC	6 cut(s)	827, 857, 2420, 2450, 3272, 3302
Bsc4I	CCNNNNNNNGG	6 cut(s)	542, 710, 1151, 2384, 2446, 3140
Bse118I	RCCGGY	1 cut(s)	1306
Bse1I	ACTGG	5 cut(s)	811, 1012, 1538, 2441, 3275
Bse3DI	GCAATG	8 cut(s)	10, 744, 1080, 1230, 1388, 2388, 2487, 3108
Bse8I	GATNNNNATC	3 cut(s)	2571, 3071, 3356
BseAI	TCCGGA	3 cut(s)	265, 445, 1706
BseBI	CCWGG	3 cut(s)	1377, 3185, 3257
BseCI	ATCGAT	1 cut(s)	1329
BseDI	CCNNGG	8 cut(s)	540, 1181, 1212, 1269, 1800, 2379, 3016, 3237
BseGI	GGATG	6 cut(s)	679, 2047, 2149, 2712, 2827, 3026
BseJI	GATNNNNATC	3 cut(s)	2571, 3071, 3356
BseLI	CCNNNNNNNGG	6 cut(s)	542, 710, 1151, 2384, 2446, 3140
BseMI	GCAATG	8 cut(s)	10, 744, 1080, 1230, 1388, 2388, 2487, 3108
BseNI	ACTGG	5 cut(s)	811, 1012, 1538, 2441, 3275
BseRI	GAGGAG	2 cut(s)	848, 2199
BseSI	GKGCMC	1 cut(s)	1005
BseX3I	CGGCCG	1 cut(s)	1435
BseXI	GCAGC	9 cut(s)	853, 1231, 1582, 1858, 2308, 2386, 2921, 3103, 3304
BseYI	CCCAGC	2 cut(s)	1665, 2716
BsgI	GTGCAG	2 cut(s)	1017, 1148
Bsh1236I	CGCG	2 cut(s)	569, 3197
Bsh1285I	CGRYCG	1 cut(s)	1438
BshFI	GGCC	5 cut(s)	520, 1437, 1975, 3040, 3464
BshNI	GGYRCC	3 cut(s)	1027, 2436, 3430
BshVI	ATCGAT	1 cut(s)	1329
BsiEI	CGRYCG	1 cut(s)	1438
BsiHKAI	GWGCWC	3 cut(s)	237, 1005, 1680
BsiHKCI	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
BsiSI	CCGG	5 cut(s)	266, 446, 1145, 1307, 1707
BslFI	GGGAC	5 cut(s)	733, 1544, 1547, 2292, 2849
BslI	CCNNNNNNNGG	6 cut(s)	542, 710, 1151, 2384, 2446, 3140
BsmAI	GTCTC	9 cut(s)	191, 232, 936, 1192, 1479, 2048, 2545, 2983, 3391
BsmBI	CGTCTC	1 cut(s)	232
BsmFI	GGGAC	5 cut(s)	733, 1544, 1547, 2292, 2849
BsmI	GAATGC	2 cut(s)	2674, 3270
BsnI	GGCC	5 cut(s)	520, 1437, 1975, 3040, 3464
Bso31I	GGTCTC	4 cut(s)	1192, 1479, 2048, 2545
BsoBI	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
Bsp119I	TTCGAA	3 cut(s)	419, 1881, 2740
Bsp1286I	GDGCHC	4 cut(s)	237, 1005, 1323, 1680
Bsp13I	TCCGGA	3 cut(s)	265, 445, 1706
Bsp1720I	GCTNAGC	1 cut(s)	627
Bsp19I	CCATGG	1 cut(s)	540
BspANI	GGCC	5 cut(s)	520, 1437, 1975, 3040, 3464
BspDI	ATCGAT	1 cut(s)	1329
BspEI	TCCGGA	3 cut(s)	265, 445, 1706
BspFNI	CGCG	2 cut(s)	569, 3197
BspHI	TCATGA	1 cut(s)	435
BspLI	GGNNCC	8 cut(s)	721, 1029, 1580, 1658, 1711, 2280, 2438, 3432
BspMAI	CTGCAG	2 cut(s)	1876, 3121
BspMI	ACCTGC	3 cut(s)	490, 1039, 3110
BspOI	GCTAGC	1 cut(s)	1314
BspPI	GGATC	9 cut(s)	437, 983, 1126, 1281, 1387, 1545, 1573, 1586, 3337
BspQI	GCTCTTC	1 cut(s)	3522
BspT104I	TTCGAA	3 cut(s)	419, 1881, 2740
BspT107I	GGYRCC	3 cut(s)	1027, 2436, 3430
BspTI	CTTAAG	1 cut(s)	382
BspTNI	GGTCTC	4 cut(s)	1192, 1479, 2048, 2545
BsrBI	CCGCTC	3 cut(s)	2271, 2276, 3515
BsrDI	GCAATG	8 cut(s)	10, 744, 1080, 1230, 1388, 2388, 2487, 3108
BsrFI	RCCGGY	1 cut(s)	1306
BsrI	ACTGG	5 cut(s)	811, 1012, 1538, 2441, 3275
BssAI	RCCGGY	1 cut(s)	1306
BssECI	CCNNGG	8 cut(s)	540, 1181, 1212, 1269, 1800, 2379, 3016, 3237
BssNI	GRCGYC	1 cut(s)	1686
BssSI	CACGAG	2 cut(s)	1294, 1679
BssT1I	CCWWGG	6 cut(s)	540, 1181, 1212, 1269, 2379, 3016
Bst2BI	CACGAG	2 cut(s)	1294, 1679
Bst2UI	CCWGG	3 cut(s)	1377, 3185, 3257
Bst6I	CTCTTC	4 cut(s)	31, 384, 1912, 3522
BstACI	GRCGYC	1 cut(s)	1686
BstAFI	CTTAAG	1 cut(s)	382
BstBI	TTCGAA	3 cut(s)	419, 1881, 2740
BstC8I	GCNNGC	3 cut(s)	237, 1312, 2760
BstDSI	CCRYGG	1 cut(s)	540
BstENI	CCTNNNNNAGG	1 cut(s)	3138
BstF5I	GGATG	6 cut(s)	679, 2047, 2149, 2712, 2827, 3026
BstFNI	CGCG	2 cut(s)	569, 3197
BstH2I	RGCGCY	2 cut(s)	318, 2915
BstHHI	GCGC	3 cut(s)	317, 1888, 2914
BstMAI	GTCTC	9 cut(s)	191, 232, 936, 1192, 1479, 2048, 2545, 2983, 3391
BstMCI	CGRYCG	1 cut(s)	1438
BstMWI	GCNNNNNNNGC	9 cut(s)	377, 496, 872, 1214, 1241, 1592, 1675, 2906, 3113
BstNI	CCWGG	3 cut(s)	1377, 3185, 3257
BstNSI	RCATGY	6 cut(s)	215, 1869, 1903, 2257, 2978, 3317
BstSCI	CCNGG	4 cut(s)	1143, 1375, 3183, 3255
BstSFI	CTRYAG	3 cut(s)	1218, 1872, 3117
BstSLI	GKGCMC	1 cut(s)	1005
BstUI	CGCG	2 cut(s)	569, 3197
BstV1I	GCAGC	9 cut(s)	853, 1231, 1582, 1858, 2308, 2386, 2921, 3103, 3304
BstV2I	GAAGAC	5 cut(s)	147, 1740, 2334, 3142, 3313
BstX2I	RGATCY	6 cut(s)	1118, 1193, 1379, 1578, 2941, 3342
BstXI	CCANNNNNNTGG	2 cut(s)	1024, 2758
BstYI	RGATCY	6 cut(s)	1118, 1193, 1379, 1578, 2941, 3342
BstZI	CGGCCG	1 cut(s)	1435
Bsu15I	ATCGAT	1 cut(s)	1329
BsuI	GTATCC	2 cut(s)	1855, 2098
BsuRI	GGCC	5 cut(s)	520, 1437, 1975, 3040, 3464
BsuTUI	ATCGAT	1 cut(s)	1329
BtgI	CCRYGG	1 cut(s)	540
BtgZI	GCGATG	2 cut(s)	692, 3039
BtsCI	GGATG	6 cut(s)	679, 2047, 2149, 2712, 2827, 3026
BtsI	GCAGTG	1 cut(s)	3465
BtsIMutI	CAGTG	5 cut(s)	1005, 1020, 3282, 3444, 3465
BveI	ACCTGC	3 cut(s)	490, 1039, 3110
Cac8I	GCNNGC	3 cut(s)	237, 1312, 2760
CciI	TCATGA	1 cut(s)	435
CfoI	GCGC	3 cut(s)	317, 1888, 2914
Cfr10I	RCCGGY	1 cut(s)	1306
Cfr13I	GGNCC	6 cut(s)	320, 720, 1973, 2279, 2748, 3038
ClaI	ATCGAT	1 cut(s)	1329
CseI	GACGC	2 cut(s)	533, 2327
DrdI	GACNNNNNNGTC	1 cut(s)	959
DseDI	GACNNNNNNGTC	1 cut(s)	959
EaeI	YGGCCR	2 cut(s)	1435, 3462
EagI	CGGCCG	1 cut(s)	1435
Eam1104I	CTCTTC	4 cut(s)	31, 384, 1912, 3522
EarI	CTCTTC	4 cut(s)	31, 384, 1912, 3522
Ecl136II	GAGCTC	2 cut(s)	235, 1678
EclXI	CGGCCG	1 cut(s)	1435
Eco130I	CCWWGG	6 cut(s)	540, 1181, 1212, 1269, 2379, 3016
Eco147I	AGGCCT	1 cut(s)	520
Eco24I	GRGCYC	3 cut(s)	237, 1323, 1680
Eco31I	GGTCTC	4 cut(s)	1192, 1479, 2048, 2545
Eco32I	GATATC	1 cut(s)	1291
Eco47I	GGWCC	4 cut(s)	320, 720, 2279, 2748
Eco47III	AGCGCT	1 cut(s)	2913
Eco52I	CGGCCG	1 cut(s)	1435
Eco53kI	GAGCTC	2 cut(s)	235, 1678
Eco57I	CTGAAG	6 cut(s)	314, 672, 693, 2346, 2384, 3503
Eco88I	CYCGRG	6 cut(s)	1492, 2847, 3035, 3209, 3238, 3367
EcoICRI	GAGCTC	2 cut(s)	235, 1678
EcoNI	CCTNNNNNAGG	1 cut(s)	3138
EcoO109I	RGGNCCY	1 cut(s)	2279
EcoRI	GAATTC	1 cut(s)	3083
EcoRII	CCWGG	3 cut(s)	1375, 3183, 3255
EcoRV	GATATC	1 cut(s)	1291
EcoT14I	CCWWGG	6 cut(s)	540, 1181, 1212, 1269, 2379, 3016
EcoT22I	ATGCAT	1 cut(s)	2413
EcoT38I	GRGCYC	3 cut(s)	237, 1323, 1680
ErhI	CCWWGG	6 cut(s)	540, 1181, 1212, 1269, 2379, 3016
Esp3I	CGTCTC	1 cut(s)	232
FalI	AAGNNNNNCTT	4 cut(s)	1722, 1754, 2443, 2475
FaqI	GGGAC	5 cut(s)	733, 1544, 1547, 2292, 2849
FauI	CCCGC	2 cut(s)	1749, 2269
FauNDI	CATATG	1 cut(s)	1912
FbaI	TGATCA	4 cut(s)	411, 1164, 3357, 3382
FblI	GTMKAC	1 cut(s)	2506
FokI	GGATG	6 cut(s)	686, 2054, 2156, 2699, 2834, 3033
FriOI	GRGCYC	3 cut(s)	237, 1323, 1680
FspBI	CTAG	6 cut(s)	1182, 1311, 1523, 1832, 2468, 3419
GlaI	GCGC	3 cut(s)	316, 1887, 2913
GsaI	CCCAGC	2 cut(s)	1669, 2720
GsuI	CTGGAG	2 cut(s)	1499, 3206
HaeII	RGCGCY	2 cut(s)	318, 2915
HaeIII	GGCC	5 cut(s)	520, 1437, 1975, 3040, 3464
HapII	CCGG	5 cut(s)	266, 446, 1145, 1307, 1707
HgaI	GACGC	2 cut(s)	533, 2327
HhaI	GCGC	3 cut(s)	317, 1888, 2914
Hin1I	GRCGYC	1 cut(s)	1686
Hin6I	GCGC	3 cut(s)	315, 1886, 2912
HinP1I	GCGC	3 cut(s)	315, 1886, 2912
HincII	GTYRAC	2 cut(s)	1141, 2542
HindII	GTYRAC	2 cut(s)	1141, 2542
HindIII	AAGCTT	3 cut(s)	339, 369, 629
HpaII	CCGG	5 cut(s)	266, 446, 1145, 1307, 1707
HphI	GGTGA	9 cut(s)	773, 946, 1022, 1166, 1351, 2295, 2644, 2693, 3383
Hpy166II	GTNNAC	8 cut(s)	792, 1003, 1141, 1648, 1684, 2474, 2507, 2542
Hpy8I	GTNNAC	8 cut(s)	792, 1003, 1141, 1648, 1684, 2474, 2507, 2542
Hpy99I	CGWCG	4 cut(s)	278, 1691, 2360, 2600
HpyAV	CCTTC	7 cut(s)	90, 175, 717, 1945, 2466, 3214, 3296
HpyCH4IV	ACGT	2 cut(s)	1686, 2598
HpyF10VI	GCNNNNNNNGC	9 cut(s)	377, 496, 872, 1214, 1241, 1592, 1675, 2906, 3113
HpySE526I	ACGT	2 cut(s)	1686, 2598
Hsp92I	GRCGYC	1 cut(s)	1686
HspAI	GCGC	3 cut(s)	315, 1886, 2912
Kpn2I	TCCGGA	3 cut(s)	265, 445, 1706
KpnI	GGTACC	2 cut(s)	2440, 3434
Ksp22I	TGATCA	4 cut(s)	411, 1164, 3357, 3382
LguI	GCTCTTC	1 cut(s)	3522
LmnI	GCTCC	6 cut(s)	206, 478, 623, 1939, 2273, 2650
Lsp1109I	GCAGC	9 cut(s)	853, 1231, 1582, 1858, 2308, 2386, 2921, 3103, 3304
LweI	GCATC	8 cut(s)	664, 2032, 2298, 2398, 2572, 3011, 3081, 3364
MaeI	CTAG	6 cut(s)	1182, 1311, 1523, 1832, 2468, 3419
MaeII	ACGT	2 cut(s)	1686, 2598
MaeIII	GTNAC	4 cut(s)	531, 1339, 1766, 2681
MbiI	CCGCTC	3 cut(s)	2271, 2276, 3515
MfeI	CAATTG	1 cut(s)	31
MflI	RGATCY	6 cut(s)	1118, 1193, 1379, 1578, 2941, 3342
MhlI	GDGCHC	4 cut(s)	237, 1005, 1323, 1680
MlsI	TGGCCA	1 cut(s)	3464
MluNI	TGGCCA	1 cut(s)	3464
MlyI	GAGTC	7 cut(s)	475, 694, 851, 1033, 1569, 2303, 2678
MmeI	TCCRAC	2 cut(s)	893, 2726
Mox20I	TGGCCA	1 cut(s)	3464
Mph1103I	ATGCAT	1 cut(s)	2413
MroI	TCCGGA	3 cut(s)	265, 445, 1706
MroXI	GAANNNNTTC	1 cut(s)	1371
MscI	TGGCCA	1 cut(s)	3464
MseI	TTAA	7 cut(s)	383, 642, 1515, 1671, 2946, 3161, 3504
MslI	CAYNNNNRTG	9 cut(s)	434, 1008, 1022, 1172, 2186, 2258, 2679, 3275, 3506
Msp20I	TGGCCA	1 cut(s)	3464
MspA1I	CMGCKG	2 cut(s)	1063, 1543
MspCI	CTTAAG	1 cut(s)	382
MspI	CCGG	5 cut(s)	266, 446, 1145, 1307, 1707
MspR9I	CCNGG	4 cut(s)	1145, 1377, 3185, 3257
MunI	CAATTG	1 cut(s)	31
Mva1269I	GAATGC	2 cut(s)	2674, 3270
MvaI	CCWGG	3 cut(s)	1377, 3185, 3257
MvnI	CGCG	2 cut(s)	569, 3197
MwoI	GCNNNNNNNGC	9 cut(s)	377, 496, 872, 1214, 1241, 1592, 1675, 2906, 3113
NciI	CCSGG	1 cut(s)	1145
NcoI	CCATGG	1 cut(s)	540
NdeI	CATATG	1 cut(s)	1912
NheI	GCTAGC	1 cut(s)	1310
NlaIV	GGNNCC	8 cut(s)	721, 1029, 1580, 1658, 1711, 2280, 2438, 3432
NmeAIII	GCCGAG	2 cut(s)	291, 1413
NmuCI	GTSAC	3 cut(s)	1339, 1766, 2681
NsiI	ATGCAT	1 cut(s)	2413
NspI	RCATGY	6 cut(s)	215, 1869, 1903, 2257, 2978, 3317
NspV	TTCGAA	3 cut(s)	419, 1881, 2740
OliI	CACNNNNGTG	1 cut(s)	1008
PaeR7I	CTCGAG	1 cut(s)	3209
PagI	TCATGA	1 cut(s)	435
PaqCI	CACCTGC	1 cut(s)	1039
PceI	AGGCCT	1 cut(s)	520
PciI	ACATGT	3 cut(s)	1865, 1899, 2974
PciSI	GCTCTTC	1 cut(s)	3522
PcsI	WCGNNNNNNNCGW	1 cut(s)	1686
PctI	GAATGC	2 cut(s)	2674, 3270
PdmI	GAANNNNTTC	1 cut(s)	1371
PfoI	TCCNGGA	2 cut(s)	1375, 3255
PleI	GAGTC	7 cut(s)	475, 694, 850, 1033, 1568, 2303, 2678
PpsI	GAGTC	7 cut(s)	475, 694, 850, 1033, 1568, 2303, 2678
PpuMI	RGGWCCY	1 cut(s)	2279
PscI	ACATGT	3 cut(s)	1865, 1899, 2974
PsiI	TTATAA	1 cut(s)	3440
Psp124BI	GAGCTC	2 cut(s)	237, 1680
Psp5II	RGGWCCY	1 cut(s)	2279
Psp6I	CCWGG	3 cut(s)	1375, 3183, 3255
PspFI	CCCAGC	2 cut(s)	1665, 2716
PspGI	CCWGG	3 cut(s)	1375, 3183, 3255
PspN4I	GGNNCC	8 cut(s)	721, 1029, 1580, 1658, 1711, 2280, 2438, 3432
PspPI	GGNCC	6 cut(s)	320, 720, 1973, 2279, 2748, 3038
PspPPI	RGGWCCY	1 cut(s)	2279
PspXI	VCTCGAGB	1 cut(s)	3209
PsrI	GAACNNNNNNTAC	2 cut(s)	2783, 2815
PstI	CTGCAG	2 cut(s)	1876, 3121
PsuI	RGATCY	6 cut(s)	1118, 1193, 1379, 1578, 2941, 3342
PvuII	CAGCTG	1 cut(s)	1543
RseI	CAYNNNNRTG	9 cut(s)	434, 1008, 1022, 1172, 2186, 2258, 2679, 3275, 3506
SacI	GAGCTC	2 cut(s)	237, 1680
SapI	GCTCTTC	1 cut(s)	3522
SaqAI	TTAA	7 cut(s)	383, 642, 1515, 1671, 2946, 3161, 3504
Sau96I	GGNCC	6 cut(s)	320, 720, 1973, 2279, 2748, 3038
SchI	GAGTC	7 cut(s)	475, 694, 851, 1033, 1569, 2303, 2678
ScrFI	CCNGG	4 cut(s)	1145, 1377, 3185, 3257
SduI	GDGCHC	4 cut(s)	237, 1005, 1323, 1680
SfaNI	GCATC	8 cut(s)	664, 2032, 2298, 2398, 2572, 3011, 3081, 3364
SfcI	CTRYAG	3 cut(s)	1218, 1872, 3117
Sfr274I	CTCGAG	1 cut(s)	3209
SfuI	TTCGAA	3 cut(s)	419, 1881, 2740
SinI	GGWCC	4 cut(s)	320, 720, 2279, 2748
SlaI	CTCGAG	1 cut(s)	3209
SmiMI	CAYNNNNRTG	9 cut(s)	434, 1008, 1022, 1172, 2186, 2258, 2679, 3275, 3506
SmlI	CTYRAG	8 cut(s)	366, 382, 929, 1189, 2165, 2266, 3088, 3209
SmoI	CTYRAG	8 cut(s)	366, 382, 929, 1189, 2165, 2266, 3088, 3209
SseBI	AGGCCT	1 cut(s)	520
SspI	AATATT	1 cut(s)	985
SspMI	CTAG	6 cut(s)	1182, 1311, 1523, 1832, 2468, 3419
SstI	GAGCTC	2 cut(s)	237, 1680
StuI	AGGCCT	1 cut(s)	520
StyD4I	CCNGG	4 cut(s)	1143, 1375, 3183, 3255
StyI	CCWWGG	6 cut(s)	540, 1181, 1212, 1269, 2379, 3016
TaiI	ACGT	2 cut(s)	1689, 2601
TatI	WGTACW	1 cut(s)	1902
TauI	GCSGC	1 cut(s)	1066
Tru1I	TTAA	7 cut(s)	383, 642, 1515, 1671, 2946, 3161, 3504
Tru9I	TTAA	7 cut(s)	383, 642, 1515, 1671, 2946, 3161, 3504
TscAI	CASTG	5 cut(s)	1012, 1027, 3282, 3451, 3472
TseFI	GTSAC	3 cut(s)	1339, 1766, 2681
TseI	GCWGC	9 cut(s)	866, 1244, 1595, 1871, 2321, 2374, 2909, 3116, 3317
Tsp45I	GTSAC	3 cut(s)	1339, 1766, 2681
TspDTI	ATGAA	9 cut(s)	695, 1190, 1421, 1616, 1628, 1638, 1899, 2804, 3315
TspGWI	ACGGA	3 cut(s)	770, 1061, 1802
TspRI	CASTG	5 cut(s)	1012, 1027, 3282, 3451, 3472
Vha464I	CTTAAG	1 cut(s)	382
VneI	GTGCAC	1 cut(s)	1001
VpaK11BI	GGWCC	4 cut(s)	320, 720, 2279, 2748
XagI	CCTNNNNNAGG	1 cut(s)	3138
XapI	RAATTY	2 cut(s)	2000, 3083
XceI	RCATGY	6 cut(s)	215, 1869, 1903, 2257, 2978, 3317
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1237, 1333, 3181
XhoI	CTCGAG	1 cut(s)	3209
XmaJI	CCTAGG	1 cut(s)	1181
XmiI	GTMKAC	1 cut(s)	2506
XmnI	GAANNNNTTC	1 cut(s)	1371
XspI	CTAG	6 cut(s)	1182, 1311, 1523, 1832, 2468, 3419
ZraI	GACGTC	1 cut(s)	1687
Zsp2I	ATGCAT	1 cut(s)	2413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.