Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

36043611 .. 36048673

Gene Structure 5063 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018786

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGGGCCATTGCATGGAGAGGACTCTGTCTTTGAAGCAGCCGCCGATTTTGGCTCAGTCCAAACCCTCCTCCTATTCGTCGAAACCGAACCATTTCCGCTTCCTCCTCTACTCCAAGAAGCTTGGCGCCCTACCGTCGCTCAGCCTCCGGTCATGGCCGCAGTCGCAGCGTCATCGTCCGAGTTGCCGAGTCAACTCTGTTCGGAGCGAGTCGTTGTCCGACGGCGGCGTGGCGGAGAAGAAAGAAGAAGCGAAGGCGGCGCCGATGAAGGTGGGGAAGAGGACGGACTTGAAGAAGATTATGATACTGGGCGCCGGGCCGATTGTGATCGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAAGCTTTGAAGGAGGAAGGATACGAGGTCGTTTTGATCAATTCCAACCCGGCTACGATCATGACCGACCCGGATTTGGCTGATAGGACCTACATTACGCCCATGACGCCGGAGTTGGTGGAGCAGGTGCTTGAAAAGGAGCGGCCGGACGCTCTTTTGCCGACGATGGGAGGCCAGACGGCGTTGAACCTGGCGGTGGCGTTGGCCGAAAGCGGTGTGCTGGAGAAGTATGGTGTGGAGTTGATTGGAGCTAAGCTGGAGGCCATTAAGAAAGCTGAGGATAGAGAGCTGTTCAAGCAGGCGATGAAGAACATTGGGGTTGAAACGCCGCCGTCTGGAATTGCCAACACCTTGGAGGAATGTGTGGAAATTGCGAAAGAGATTGGTGAGTTTCCCTTGATCATTAGGCCAGCTTTTACGTTGGGAGGGACGGGAGGTGGTATTGCATATAATAAGGAGGAATTTGAGACTATTTGTAAGGCCGGGCTGGCGGCGAGTTTGACCTCTCAGGTTTTGGTGGAGAAGTCACTGTTAGGGTGGAAGGAATATGAGCTTGAGGTGATGAGGGACTTGGCAGATAATGTGGTTATTATTTGCTCCATTGAGAATATTGACCCGATGGGGGTTCATACGGGAGACTCTATCACCGTGGCGCCTGCTCAGACTTTAACTGATAAGGAGTATCAGCGGCTGAGGGACTATTCGATAGCTATAATAAGAGAGATTGGAGTGGAATGTGGTGGTTCTAATGTGCAGTTTGCTGTGAATCCTCAAGACGGGGAGGTTATGGTGATTGAGATGAATCCAAGGGTGTCCCGGTCCTCGGCTCTGGCATCCAAAGCAACTGGTTTTCCGATAGCGAAAATGGCTGCTAGGTTGTCAGTTGGATATTCCTTGGATCAAATTCCCAATGACATTACAAAGAAGACACCAGCTAGTTTTGAGCCTTCCATAGACTACGTGGTAACAAAGATTCCCCGGTTTGCTTTTGAAAAGTTTCCTGGTTCAGAGCCAATACTGACGACTCAGATGAAATCTGTCGGTGAGTCAATGGCACTAGGGCGTACATTTCAAGAATCCTTTCAGAAAGCAGTTCGGTCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGGAAAATTAAGGAACTAGACTGGGATTGGGAGCAATTGAAATATAGTCTCCGAGTCCCTAACCCAGATCGTATCCATGCTGTGTATGCTGCAATGAAGAAGGGAATGAAGATAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAAGAATTGGTGGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGACTTCCATGAAGTGAAAAGAAGAGGCTTCAGTGACAAACAAATAGCTTTTGCCACTAAATCCTCTGAAAATGAAGTGCGGTTGAAGAGATTATCATTGGGTGTCACTCCAGCATATAAGCGGGTAGATACGTGTGCCGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCGTCTTATGACTTTGAGTGTGAATCCACTCCCACCCAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAAACAATCATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACAAGTGACCGTTTATATTTTGAGCCCCTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTTGGAGGTCAAACACCTCTGAAGCTAGCACTTCCCCTCCAGCGGTATTTAGATGAAAACAAGCCTAGATGTGCAAGTGGTGCTGGACTTGTTCGCATTTGGGGTACCACACCTGACTCCATAGATGCTGCTGAGGACAGGGAAAAGTTCAATGCAATTCTGAACGAGTTAAAGATTGAACAGCCAAAAGGAGGAATTGCCAAGAGTGAAGCAGATGCTCTTGCCATTGCCACAAACATTGGCTACCCTGTCGTTGTCCGACCCTCCTATGTATTGGGCGGCCGTGCCATGGAGATAGTATATAGTGATGAAAAACTTGTGACCTACCTAGAAACTGCTGTTGAGGTGGACCCGGAACGTCCTGTATTGATTGACAGGTATCTATCCGATGCCATTGAGATTGATGTTGATGCACTCGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATCGAGCAGGCTGGGGTCCATTCTGGTGATTCTGCTTGCTCGATTCCGACAAAAACCATCCCAGAGTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCTAAGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGAGATGTTTATTTACTCGAGGCAAATCCTCGTGCTTCCCGTACGGTCCCTTTTGTGTCCAAGGCAATTGGACATCCGTTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGATCTTGGTTTCACAAAAGAAGTCATTCCTGCACATATGTCAGTGAAAGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTATTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCAATTGCATTTGCCAAGGCTCAGATTGCTGCTGGGCAGGCGCTACCACTTTCAGGCACTGTGTTTTTGAGCTTAAATGACTTGACCAAACCCCATCTTGAGAGAATTGCAAAGGCATTTTTAGGCCTTGGATTTAAGATTGTTTCAACTTCAGGAACAGCTCATGTTCTTGAACTAGCGAAAATTCCGGTGGAACGAGTGCTGAAGCTACATGAGGGGAGGCCTAATGCTGGTGACACTGTTGCTAATGGGCAAATCCAGTTGATGGTGATTACAAGTTCTGGTGATGCATTCGATCAGATTGATGGACGGCAGCTAAGGAGGACGGCCCTTGCTTACAAGATTCCAGTAATAACAACAGTTGCTGGAGCTTTGGCAACTGCAGAAGCAATAAAGAGCTTAAAGACCTGCTCCATTAAGATGATTGCACTTCAGGACTTCTTTGATGACGAGAATAAAGCTGTCAGTGATAAAAAGTTGCAGTCTTCCTCTGTCCCTCTAATGAGTTCAGTGTTGGGTTTTTCTTGGCGTCGTTCAGTGGTGGTACAATTAGACAACATTGGTGCTGTATTCTCTTGTGCACAGACATTTACTTATCCAAACTGCTTTACAGCACAGTTGAATAATCAGAGAGCTTCTGCATTAATGAAAAAACTTGATTTAGGTTTTACTTCTACTTCTTGCCCATTTCATGCCAATGCTACTTCAGCTGGAACACAAGGGGGTAGTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1273

Amino Acids

139.15

Weight (kDa)

5.79

Isoelectric Point (pI)

38.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	97 - 214	6.1e-49	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	218 - 425	1.6e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	517 - 595	7.1e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	647 - 772	2.2e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	776 - 979	5.3e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	784 - 950	8e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	803 - 948	3.3e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1062 - 1147	2.7e-19	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	487
Acc36I	ACCTGC	2 cut(s)	487, 3500
Acc65I	GGTACC	1 cut(s)	2294
AccB1I	GGYRCC	5 cut(s)	125, 259, 311, 1024, 2294
AccB7I	CCANNNNNTGG	2 cut(s)	13, 3349
AccBSI	CCGCTC	1 cut(s)	514
AclWI	GGATC	2 cut(s)	1278, 2186
AcoI	YGGCCR	4 cut(s)	155, 515, 576, 2470
AcsI	RAATTY	6 cut(s)	833, 1275, 1623, 1997, 2065, 3267
AcuI	CTGAAG	7 cut(s)	1744, 2018, 2231, 3219, 3308, 3500, 3774
AcyI	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
AfaI	GTAC	5 cut(s)	1438, 1901, 2296, 2857, 3630
AflIII	ACRYGT	1 cut(s)	1862
AjnI	CCWGG	4 cut(s)	561, 1372, 1698, 2910
Alw21I	GWGCWC	2 cut(s)	2652, 3667
Alw26I	GTCTC	4 cut(s)	833, 1002, 1556, 2934
Alw44I	GTGCAC	1 cut(s)	3663
AlwI	GGATC	2 cut(s)	1278, 2186
AlwNI	CAGNNNCTG	3 cut(s)	1063, 3143, 3449
Ama87I	CYCGRG	2 cut(s)	355, 2831
ApaLI	GTGCAC	1 cut(s)	3663
ApeKI	GCWGC	7 cut(s)	37, 166, 1241, 1592, 2318, 3113, 3397
ApoI	RAATTY	6 cut(s)	833, 1275, 1623, 1997, 2065, 3267
ArsI	GACNNNNNNTTYG	2 cut(s)	13, 45
AseI	ATTAAT	1 cut(s)	3728
Asp700I	GAANNNNTTC	5 cut(s)	92, 1368, 1452, 2961, 2991
Asp718I	GGTACC	1 cut(s)	2294
AspLEI	GCGC	5 cut(s)	128, 262, 314, 1027, 3127
AsuC2I	CCSGG	7 cut(s)	316, 422, 443, 856, 1189, 1351, 2543
AsuNHI	GCTAGC	1 cut(s)	2215
AvaI	CYCGRG	2 cut(s)	355, 2831
AvaII	GGWCC	6 cut(s)	459, 1191, 1970, 2539, 2668, 2860
BaeGI	GKGCMC	1 cut(s)	3667
BanI	GGYRCC	5 cut(s)	125, 259, 311, 1024, 2294
BanII	GRGCYC	1 cut(s)	2127
BauI	CACGAG	1 cut(s)	2844
BbsI	GAAGAC	2 cut(s)	1304, 3561
Bbv12I	GWGCWC	2 cut(s)	2652, 3667
BbvCI	CCTCAGC	3 cut(s)	648, 1064, 2322
BbvI	GCAGC	7 cut(s)	49, 178, 1228, 1579, 2305, 3100, 3409
BccI	CCATC	9 cut(s)	532, 985, 1488, 2168, 2717, 3058, 3186, 3343, 3383
BceAI	ACGGC	6 cut(s)	238, 567, 688, 2457, 3410, 3426
BcgI	CGANNNNNNTGC	2 cut(s)	155, 189
BciT130I	CCWGG	4 cut(s)	563, 1374, 1700, 2912
BciVI	GTATCC	2 cut(s)	386, 1586
BclI	TGATCA	2 cut(s)	408, 771
BcnI	CCSGG	7 cut(s)	316, 422, 443, 856, 1189, 1351, 2543
BcoDI	GTCTC	4 cut(s)	833, 1002, 1556, 2934
BfaI	CTAG	9 cut(s)	1245, 1308, 1430, 1520, 1704, 2216, 2256, 2519, 3260
BfmI	CTRYAG	1 cut(s)	3465
BfoI	RGCGCY	5 cut(s)	129, 263, 315, 1028, 3128
BfuAI	ACCTGC	2 cut(s)	487, 3500
BfuI	GTATCC	2 cut(s)	386, 1586
BglI	GCCNNNNNGGC	1 cut(s)	860
BlpI	GCTNAGC	2 cut(s)	140, 624
Bme18I	GGWCC	6 cut(s)	459, 1191, 1970, 2539, 2668, 2860
BmeT110I	CYCGRG	2 cut(s)	355, 2831
BmiI	GGNNCC	9 cut(s)	127, 261, 313, 1026, 1655, 2296, 2541, 2669, 2862
BmrI	ACTGGG	2 cut(s)	317, 1534
BmsI	GCATC	7 cut(s)	1214, 2305, 2395, 2569, 2590, 3033, 3361
BmtI	GCTAGC	1 cut(s)	2219
BmuI	ACTGGG	2 cut(s)	317, 1534
BpiI	GAAGAC	2 cut(s)	1304, 3561
BpmI	CTGGAG	6 cut(s)	614, 650, 1824, 2213, 2835, 3471
Bpu10I	CCTNAGC	4 cut(s)	648, 1064, 2322, 3401
Bpu1102I	GCTNAGC	2 cut(s)	140, 624
BpuEI	CTTGAG	4 cut(s)	348, 947, 1128, 3203
BpuMI	CCSGG	7 cut(s)	316, 422, 443, 856, 1189, 1351, 2543
BsaAI	YACGTR	2 cut(s)	1333, 1863
BsaBI	GATNNNNATC	1 cut(s)	326
BsaHI	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
BsaWI	WCCGGW	2 cut(s)	147, 3271
BsaXI	ACNNNNNCTCC	2 cut(s)	2211, 2241
Bse1I	ACTGG	6 cut(s)	312, 1222, 1529, 3058, 3343, 3431
Bse3DI	GCAATG	3 cut(s)	7, 1602, 2415
Bse8I	GATNNNNATC	1 cut(s)	326
BseBI	CCWGG	4 cut(s)	563, 1374, 1700, 2912
BseGI	GGATG	7 cut(s)	1205, 1499, 1687, 1735, 2021, 2709, 2887
BseJI	GATNNNNATC	1 cut(s)	326
BseMI	GCAATG	3 cut(s)	7, 1602, 2415
BseNI	ACTGG	6 cut(s)	312, 1222, 1529, 3058, 3343, 3431
BseRI	GAGGAG	2 cut(s)	58, 95
BseSI	GKGCMC	1 cut(s)	3667
BseX3I	CGGCCG	2 cut(s)	515, 2470
BseXI	GCAGC	7 cut(s)	49, 178, 1228, 1579, 2305, 3100, 3409
BseYI	CCCAGC	2 cut(s)	2663, 3116
BsgI	GTGCAG	3 cut(s)	1145, 2207, 2952
Bsh1285I	CGRYCG	2 cut(s)	518, 2473
BshNI	GGYRCC	5 cut(s)	125, 259, 311, 1024, 2294
BsiEI	CGRYCG	2 cut(s)	518, 2473
BsiHKAI	GWGCWC	2 cut(s)	2652, 3667
BsiHKCI	CYCGRG	2 cut(s)	355, 2831
BsiWI	CGTACG	1 cut(s)	2855
BslFI	GGGAC	8 cut(s)	373, 814, 953, 1082, 1171, 1544, 2846, 3563
BsmAI	GTCTC	4 cut(s)	833, 1002, 1556, 2934
BsmFI	GGGAC	8 cut(s)	373, 814, 953, 1082, 1171, 1544, 2846, 3563
BsmI	GAATGC	1 cut(s)	3374
BsoBI	CYCGRG	2 cut(s)	355, 2831
Bsp1286I	GDGCHC	3 cut(s)	2127, 2652, 3667
Bsp1720I	GCTNAGC	2 cut(s)	140, 624
Bsp19I	CCATGG	1 cut(s)	2478
BspHI	TCATGA	4 cut(s)	432, 1627, 2058, 2935
BspLI	GGNNCC	9 cut(s)	127, 261, 313, 1026, 1655, 2296, 2541, 2669, 2862
BspMAI	CTGCAG	1 cut(s)	3469
BspMI	ACCTGC	2 cut(s)	487, 3500
BspOI	GCTAGC	1 cut(s)	2219
BspPI	GGATC	2 cut(s)	1278, 2186
BspT107I	GGYRCC	5 cut(s)	125, 259, 311, 1024, 2294
BsrBI	CCGCTC	1 cut(s)	514
BsrDI	GCAATG	3 cut(s)	7, 1602, 2415
BsrI	ACTGG	6 cut(s)	312, 1222, 1529, 3058, 3343, 3431
BssNI	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
BssSI	CACGAG	1 cut(s)	2844
BssT1I	CCWWGG	8 cut(s)	723, 1178, 1266, 2478, 2874, 3013, 3099, 3211
Bst2BI	CACGAG	1 cut(s)	2844
Bst2UI	CCWGG	4 cut(s)	563, 1374, 1700, 2912
Bst6I	CTCTTC	4 cut(s)	272, 1747, 1811, 2937
BstACI	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
BstAPI	GCANNNNNTGC	1 cut(s)	2270
BstBAI	YACGTR	2 cut(s)	1333, 1863
BstDSI	CCRYGG	2 cut(s)	1020, 2478
BstF5I	GGATG	7 cut(s)	1205, 1499, 1687, 1735, 2021, 2709, 2887
BstH2I	RGCGCY	5 cut(s)	129, 263, 315, 1028, 3128
BstHHI	GCGC	5 cut(s)	128, 262, 314, 1027, 3127
BstMAI	GTCTC	4 cut(s)	833, 1002, 1556, 2934
BstMCI	CGRYCG	2 cut(s)	518, 2473
BstNI	CCWGG	4 cut(s)	563, 1374, 1700, 2912
BstSFI	CTRYAG	1 cut(s)	3465
BstSLI	GKGCMC	1 cut(s)	3667
BstV1I	GCAGC	7 cut(s)	49, 178, 1228, 1579, 2305, 3100, 3409
BstV2I	GAAGAC	2 cut(s)	1304, 3561
BstXI	CCANNNNNNTGG	1 cut(s)	724
BstZI	CGGCCG	2 cut(s)	515, 2470
BsuI	GTATCC	2 cut(s)	386, 1586
BtgI	CCRYGG	2 cut(s)	1020, 2478
BtgZI	GCGATG	1 cut(s)	689
BtsCI	GGATG	7 cut(s)	1205, 1499, 1687, 1735, 2021, 2709, 2887
BtsIMutI	CAGTG	9 cut(s)	899, 1768, 2985, 3051, 3141, 3321, 3556, 3600, 3627
BveI	ACCTGC	2 cut(s)	487, 3500
CaiI	CAGNNNCTG	3 cut(s)	1063, 3143, 3449
CciI	TCATGA	4 cut(s)	432, 1627, 2058, 2935
CfoI	GCGC	5 cut(s)	128, 262, 314, 1027, 3127
CseI	GACGC	4 cut(s)	158, 487, 530, 3602
Csp6I	GTAC	5 cut(s)	1437, 1900, 2295, 2856, 3629
CspCI	CAANNNNNGTGG	2 cut(s)	2410, 2445
CviQI	GTAC	5 cut(s)	1437, 1900, 2295, 2856, 3629
DinI	GGCGCC	4 cut(s)	127, 261, 313, 1026
EaeI	YGGCCR	4 cut(s)	155, 515, 576, 2470
EagI	CGGCCG	2 cut(s)	515, 2470
Eam1104I	CTCTTC	4 cut(s)	272, 1747, 1811, 2937
EarI	CTCTTC	4 cut(s)	272, 1747, 1811, 2937
EciI	GGCGGA	1 cut(s)	248
EclXI	CGGCCG	2 cut(s)	515, 2470
Eco130I	CCWWGG	8 cut(s)	723, 1178, 1266, 2478, 2874, 3013, 3099, 3211
Eco147I	AGGCCT	2 cut(s)	3210, 3307
Eco24I	GRGCYC	1 cut(s)	2127
Eco47I	GGWCC	6 cut(s)	459, 1191, 1970, 2539, 2668, 2860
Eco52I	CGGCCG	2 cut(s)	515, 2470
Eco57I	CTGAAG	7 cut(s)	1744, 2018, 2231, 3219, 3308, 3500, 3774
Eco88I	CYCGRG	2 cut(s)	355, 2831
EcoO109I	RGGNCCY	1 cut(s)	459
EcoRI	GAATTC	1 cut(s)	2065
EcoRII	CCWGG	4 cut(s)	561, 1372, 1698, 2910
EcoT14I	CCWWGG	8 cut(s)	723, 1178, 1266, 2478, 2874, 3013, 3099, 3211
EcoT22I	ATGCAT	1 cut(s)	3376
EcoT38I	GRGCYC	1 cut(s)	2127
EgeI	GGCGCC	4 cut(s)	127, 261, 313, 1026
EheI	GGCGCC	4 cut(s)	127, 261, 313, 1026
ErhI	CCWWGG	8 cut(s)	723, 1178, 1266, 2478, 2874, 3013, 3099, 3211
FalI	AAGNNNNNCTT	4 cut(s)	1718, 1750, 2979, 3011
FaqI	GGGAC	8 cut(s)	373, 814, 953, 1082, 1171, 1544, 2846, 3563
FauI	CCCGC	1 cut(s)	1845
FauNDI	CATATG	1 cut(s)	2973
FbaI	TGATCA	2 cut(s)	408, 771
FokI	GGATG	7 cut(s)	1192, 1506, 1694, 1742, 2008, 2696, 2874
FriOI	GRGCYC	1 cut(s)	2127
FspBI	CTAG	9 cut(s)	1245, 1308, 1430, 1520, 1704, 2216, 2256, 2519, 3260
GlaI	GCGC	5 cut(s)	127, 261, 313, 1026, 3126
GsaI	CCCAGC	2 cut(s)	2667, 3120
GsuI	CTGGAG	6 cut(s)	614, 650, 1824, 2213, 2835, 3471
HaeII	RGCGCY	5 cut(s)	129, 263, 315, 1028, 3128
HgaI	GACGC	4 cut(s)	158, 487, 530, 3602
HhaI	GCGC	5 cut(s)	128, 262, 314, 1027, 3127
Hin1I	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
Hin6I	GCGC	5 cut(s)	126, 260, 312, 1025, 3125
HinP1I	GCGC	5 cut(s)	126, 260, 312, 1025, 3125
HincII	GTYRAC	1 cut(s)	193
HindII	GTYRAC	1 cut(s)	193
HindIII	AAGCTT	4 cut(s)	119, 336, 366, 375
Hpy166II	GTNNAC	3 cut(s)	193, 2539, 3665
Hpy8I	GTNNAC	3 cut(s)	193, 2539, 3665
Hpy99I	CGWCG	5 cut(s)	82, 139, 224, 538, 3618
HpyAV	CCTTC	9 cut(s)	247, 262, 376, 383, 907, 1329, 1597, 1942, 2042
HpyCH4IV	ACGT	4 cut(s)	791, 1332, 1862, 2548
HpySE526I	ACGT	4 cut(s)	791, 1332, 1862, 2548
Hsp92I	GRCGYC	6 cut(s)	126, 260, 312, 479, 1025, 3613
HspAI	GCGC	5 cut(s)	126, 260, 312, 1025, 3125
KasI	GGCGCC	4 cut(s)	125, 259, 311, 1024
KpnI	GGTACC	1 cut(s)	2298
Ksp22I	TGATCA	2 cut(s)	408, 771
LmnI	GCTCC	9 cut(s)	204, 493, 511, 620, 974, 1534, 2647, 3452, 3500
Lsp1109I	GCAGC	7 cut(s)	49, 178, 1228, 1579, 2305, 3100, 3409
LweI	GCATC	7 cut(s)	1214, 2305, 2395, 2569, 2590, 3033, 3361
MaeI	CTAG	9 cut(s)	1245, 1308, 1430, 1520, 1704, 2216, 2256, 2519, 3260
MaeII	ACGT	4 cut(s)	791, 1332, 1862, 2548
MaeIII	GTNAC	7 cut(s)	897, 1336, 1763, 1834, 2105, 2509, 3317
MbiI	CCGCTC	1 cut(s)	514
MfeI	CAATTG	3 cut(s)	1538, 2880, 3087
MhlI	GDGCHC	3 cut(s)	2127, 2652, 3667
Mly113I	GGCGCC	4 cut(s)	126, 260, 312, 1025
MmeI	TCCRAC	5 cut(s)	243, 441, 1237, 2473, 2723
Mph1103I	ATGCAT	1 cut(s)	3376
MroXI	GAANNNNTTC	5 cut(s)	92, 1368, 1452, 2961, 2991
MslI	CAYNNNNRTG	4 cut(s)	2622, 2676, 3503, 3780
MspA1I	CMGCKG	3 cut(s)	1060, 2233, 3794
MunI	CAATTG	3 cut(s)	1538, 2880, 3087
Mva1269I	GAATGC	1 cut(s)	3374
MvaI	CCWGG	4 cut(s)	563, 1374, 1700, 2912
NarI	GGCGCC	4 cut(s)	126, 260, 312, 1025
NciI	CCSGG	7 cut(s)	316, 422, 443, 856, 1189, 1351, 2543
NcoI	CCATGG	1 cut(s)	2478
NdeI	CATATG	1 cut(s)	2973
NheI	GCTAGC	1 cut(s)	2215
NlaIV	GGNNCC	9 cut(s)	127, 261, 313, 1026, 1655, 2296, 2541, 2669, 2862
NmeAIII	GCCGAG	2 cut(s)	212, 1175
NmuCI	GTSAC	6 cut(s)	897, 1763, 1834, 2105, 2509, 3317
NsiI	ATGCAT	1 cut(s)	3376
PaeR7I	CTCGAG	1 cut(s)	2831
PagI	TCATGA	4 cut(s)	432, 1627, 2058, 2935
PaqCI	CACCTGC	1 cut(s)	487
PceI	AGGCCT	2 cut(s)	3210, 3307
PcsI	WCGNNNNNNNCGW	2 cut(s)	83, 2851
PctI	GAATGC	1 cut(s)	3374
PdmI	GAANNNNTTC	5 cut(s)	92, 1368, 1452, 2961, 2991
PfeI	GAWTC	8 cut(s)	1138, 1174, 1345, 1448, 1925, 2681, 2695, 3427
Pfl23II	CGTACG	1 cut(s)	2855
PflFI	GACNNNGTC	1 cut(s)	25
PflMI	CCANNNNNTGG	2 cut(s)	13, 3349
PluTI	GGCGCC	4 cut(s)	129, 263, 315, 1028
Ppu21I	YACGTR	2 cut(s)	1333, 1863
PpuMI	RGGWCCY	1 cut(s)	459
PshBI	ATTAAT	1 cut(s)	3728
Psp5II	RGGWCCY	1 cut(s)	459
Psp6I	CCWGG	4 cut(s)	561, 1372, 1698, 2910
PspFI	CCCAGC	2 cut(s)	2663, 3116
PspGI	CCWGG	4 cut(s)	561, 1372, 1698, 2910
PspLI	CGTACG	1 cut(s)	2855
PspN4I	GGNNCC	9 cut(s)	127, 261, 313, 1026, 1655, 2296, 2541, 2669, 2862
PspPPI	RGGWCCY	1 cut(s)	459
PspXI	VCTCGAGB	1 cut(s)	2831
PstI	CTGCAG	1 cut(s)	3469
PstNI	CAGNNNCTG	3 cut(s)	1063, 3143, 3449
PsyI	GACNNNGTC	1 cut(s)	25
PvuII	CAGCTG	1 cut(s)	3794
RsaI	GTAC	5 cut(s)	1438, 1901, 2296, 2857, 3630
RsaNI	GTAC	5 cut(s)	1437, 1900, 2295, 2856, 3629
RseI	CAYNNNNRTG	4 cut(s)	2622, 2676, 3503, 3780
SduI	GDGCHC	3 cut(s)	2127, 2652, 3667
SfaNI	GCATC	7 cut(s)	1214, 2305, 2395, 2569, 2590, 3033, 3361
SfcI	CTRYAG	1 cut(s)	3465
SfoI	GGCGCC	4 cut(s)	127, 261, 313, 1026
Sfr274I	CTCGAG	1 cut(s)	2831
SinI	GGWCC	6 cut(s)	459, 1191, 1970, 2539, 2668, 2860
SlaI	CTCGAG	1 cut(s)	2831
SmiMI	CAYNNNNRTG	4 cut(s)	2622, 2676, 3503, 3780
SmlI	CTYRAG	5 cut(s)	363, 926, 1143, 2831, 3182
SmoI	CTYRAG	5 cut(s)	363, 926, 1143, 2831, 3182
SseBI	AGGCCT	2 cut(s)	3210, 3307
SspDI	GGCGCC	4 cut(s)	125, 259, 311, 1024
SspI	AATATT	1 cut(s)	982
SspMI	CTAG	9 cut(s)	1245, 1308, 1430, 1520, 1704, 2216, 2256, 2519, 3260
StuI	AGGCCT	2 cut(s)	3210, 3307
StyI	CCWWGG	8 cut(s)	723, 1178, 1266, 2478, 2874, 3013, 3099, 3211
TaiI	ACGT	4 cut(s)	794, 1335, 1865, 2551
TaqI	TCGA	8 cut(s)	80, 348, 1076, 1978, 2655, 2693, 2832, 3378
TaqII	GACCGA	2 cut(s)	452, 1458
TatI	WGTACW	1 cut(s)	1899
TfiI	GAWTC	8 cut(s)	1138, 1174, 1345, 1448, 1925, 2681, 2695, 3427
TscAI	CASTG	9 cut(s)	906, 1768, 2985, 3058, 3148, 3328, 3556, 3600, 3627
TseFI	GTSAC	6 cut(s)	897, 1763, 1834, 2105, 2509, 3317
TseI	GCWGC	7 cut(s)	37, 166, 1241, 1592, 2318, 3113, 3397
Tsp45I	GTSAC	6 cut(s)	897, 1763, 1834, 2105, 2509, 3317
TspGWI	ACGGA	2 cut(s)	299, 2880
TspRI	CASTG	9 cut(s)	906, 1768, 2985, 3058, 3148, 3328, 3556, 3600, 3627
Tth111I	GACNNNGTC	1 cut(s)	25
Van91I	CCANNNNNTGG	2 cut(s)	13, 3349
VneI	GTGCAC	1 cut(s)	3663
VpaK11BI	GGWCC	6 cut(s)	459, 1191, 1970, 2539, 2668, 2860
VspI	ATTAAT	1 cut(s)	3728
XapI	RAATTY	6 cut(s)	833, 1275, 1623, 1997, 2065, 3267
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1330
XhoI	CTCGAG	1 cut(s)	2831
XmnI	GAANNNNTTC	5 cut(s)	92, 1368, 1452, 2961, 2991
XspI	CTAG	9 cut(s)	1245, 1308, 1430, 1520, 1704, 2216, 2256, 2519, 3260
Zsp2I	ATGCAT	1 cut(s)	3376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.