Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Reverse (-)

37285346 .. 37291414

Gene Structure 6069 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG326600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3576 bp

ATGGGCCATTGCTGCATGAATCACACCGACACTGCTCTCTCTTCCATTTTTGCTCAATCCAACCTCTCCTATCTCTCCACACAACCAAACCCCTTCCGCTTCTTCCTCTGCTCCAAGACCCACCGAAACGCCGCCGTTCCCCCTCTCAGACTCCGCTCATGGCCCACTTCCACGCAGCCGAGCCTCCGAGTAGCCACCCCGGTTCGGAGCGCCTCCAAAGAAGCAGCAGCAGCGCCGCCCAAGAAGCAGCAGCAGCAGCTGGGGAAAAGGACCGACCTGAAGAAGATTCTGATACTGGGCGCCGGTCCCATTGTGATTGGCCAAGCCTGCGAGTTCGATTATTCAGGGACTCAAGCTTGTAAGGCTTTGAAAGAAGAAGGATACGACGTCGTTTTGATCAATTCCAACCCGGCCACCATCATGACCGACCCCGATTTGGCCGACCGGACTTACATTACGCCGATGACGCCGGAGCTGGTGGAGCAAGTCCTCGAAAAGGAGCGCCCCGACGCTCTCCTCCCCACCATGGGAGGCCAGACGGCGCTGAACCTCGCCGTTGCGTTAGCCGAGAGCGGTGCGCTTGAAAAGTATGGGGTGGAGTTGATCGGAGCCAAGCTGGAGGCCATAAAGAAAGCAGAGGATAGGGATTTGTTCAAGCAGGCCATGAACAACATTGGGATCAAAACGCCGCCGTCTGGAATTGCCAACACTTATGAGGAATGCGTCGAAATCGCCAATGAGATTGGTGAGTTTCCCTTGATCATTAGGCCGGCTTTTACTCTAGGAGGGACAGGGGGTGGAATTGCTTATAATAAGGAGGAGTTTGAGACCATCTGTAAGTCGGGGCTGGCGGCGAGCTTGACTTCTCAGATTTTGGTGGAGAAGTCATTGTTGGGGTGGAAGGAGTATGAGCTTGAGGTCATGAGGGACTTGGCAGACAATGTGGTTATTATTTGCTCCATTGAGAATATTGACCCAATGGGGGTTCATACTGGGGATTCTATCACCGTGGCGCCTGCGCAGACTTTGACTGATAAGGAGTATCAGCGGCTAAGAGACTATTCCATTGCTATAATAAGGGAGATTGGAGTGGAGTGTGGTGGCTCTAATGTGCAGTTTGCGGTGAATCCCAAAGATGGGGAGGTTATGGTGATTGAGATGAATCCTAGGGTGTCGCGGTCCTCGGCTCTCGCATCCAAAGCTACTGGATTTCCGATAGCGAAAATGGCTGCCAAGTTGTCAGTTGGGTATTCATTGGATCAAATTCCCAATGACATTACAAAGAAGACACCGGCTAGTTTTGAACCTTCCATAGACTATGTGGTAACAAAGATTCCCAGGTTTGCTTTTGAGAAATTTCCTGGTTCACAGCCAATATTGACGACCCAGATGAAATCTGTTGGTGAGTCAATGGCTCTGGGTCGTACATTTCAAGAGTCCTTTCAGAAGGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGCAAAATTAAGGAACTGGACTGGGGTTGGGACAAGTTGAAATATAGCCTCCGAGTCCCTAACCCAGATCGAATCCATGCCGTATATGCTGCAATGAAGAAGGGGATGAAGGTAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTCAAGGAGTTAGTAGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGAGTTCTACGAAGTGAAGAAAAGAGGCTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCTTCTGAAAATGAAGTGCGGTTGAAGAGACTATTGTTAGGTGTTACTCCAGCATATAAGCGGGTAGATACGTGCGCTGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCTTCCTATGACTTTGAGTGTGAATCAGTTCCGACCAAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAAACAATTATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACGAGCGACCGTCTATATTTTGAGCCACTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTCGGAGGACAAACACCTCTGAAGCTGGCACTTCCCCTCCAGCAGTATTTAGACGAAAACAAGCCGAGATGTGCAAGTGGGATTGGACATGTGCGCATTTGGGGTACAACACCTGACTCCATAGATGCTGCTGAGGACAGGGAGAAGTTTAATGCAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGAATTGCAAAGAGCGAAGCAGATGCTCTTGCAATTGCAACAGACATTGGCTACCCTGTTGTTGTCCGACCTTCCTATGTATTAGGCGGCCGTGCCATGGAGATAGTCTATAGTGATGAAAAACTTGTGACCTACCTTGAAACTGCTGTTGAGGTGGACCCAGAACGCCCTGTATTGATAGACAGGTATCTGTCAGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATTGAGCAGGCTGGGGTCCATTCTGGTGACTCTGCTTGCTCAATTCCGACAAAAACCATCCCAGCTTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCCGAGAGGCTGAATGTGTGCGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGTGATGTTTATTTACTTGAGGCAAATCCTCGTGCTTCCCGTACAGTCCCATTTGTGTCCAAGGCAATTGGACATCCATTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGACCTTAATTTCACAAAAGAAGTCATTCCTGCACATGTGTCAGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTACTGCTTGGGCCCGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCCATTGCGTTTGCCAAGGCGCAGATTGCTGCTGGACAGAAGCCACCACTTTCTGGCACTGTGTTCTTGAGCTTAAATGACTTAACCAAACCTCATCTTGAAAGGATTGCAACCGCATTTTTAGGCCTTGGATTTAAGATTGTCTCAACTTCAGGGACAGCTCATTTTCTAGAATTAGCGAAAATTCCCGTGGAACGAGTGCTGAAGCTGCATGAGGGGCGACCTAATGCTGGTGACATGGTTGCAAATGGACAAATCCAGTTGATGGTGATCACAAGTTCTGGGGATGCACATGATCAAATTGATGGACGGCAGCTAAGGAGGATGGCTCTTGCTTACAAGATTCCAGTAATTACCACAGTTGCCGGAGCATTGGCCACTGCAGAAGCAATAAAGAGTCTAAAGTCCAGCTCCATTAAAATGATTGCTCTTCAGGACTTCTTTGATGACGAGAACAAAGCTCTCGGTAATAAGAAGTTGCAGTCAGCCTCGTCCTCTATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1191

Amino Acids

130.32

Weight (kDa)

5.64

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	93 - 210	1e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	214 - 421	4.4e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	513 - 591	4.6e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	644 - 768	8.5e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	772 - 975	1.2e-37	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	780 - 946	8e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	799 - 944	1.2e-06	D-ala D-ala ligase C-terminus
MGS	PF02142	1058 - 1143	3.7e-20	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
AatII	GACGTC	1 cut(s)	390
Acc16I	TGCGCA	2 cut(s)	1020, 2273
AccB1I	GGYRCC	2 cut(s)	299, 1012
AccB7I	CCANNNNNTGG	2 cut(s)	3125, 3337
AccBSI	CCGCTC	2 cut(s)	156, 573
AccII	CGCG	1 cut(s)	1177
AclWI	GGATC	3 cut(s)	686, 1266, 2174
AcoI	YGGCCR	6 cut(s)	319, 411, 438, 2458, 2745, 3447
AcsI	RAATTY	6 cut(s)	1263, 1355, 1611, 1985, 2053, 3255
AcuI	CTGAAG	7 cut(s)	299, 1732, 2006, 2219, 3207, 3296, 3488
AcyI	GRCGYC	4 cut(s)	300, 387, 467, 1013
AfaI	GTAC	5 cut(s)	1426, 1889, 2284, 2845, 3015
AflIII	ACRYGT	2 cut(s)	2266, 2959
AjnI	CCWGG	4 cut(s)	1337, 1360, 1686, 2898
AjuI	GAANNNNNNNTTGG	2 cut(s)	875, 907
Alw21I	GWGCWC	1 cut(s)	2640
Alw26I	GTCTC	5 cut(s)	821, 1050, 1801, 2922, 3220
AlwI	GGATC	3 cut(s)	686, 1266, 2174
AlwNI	CAGNNNCTG	1 cut(s)	259
Ama87I	CYCGRG	1 cut(s)	3026
ApaI	GGGCCC	1 cut(s)	3027
ApoI	RAATTY	6 cut(s)	1263, 1355, 1611, 1985, 2053, 3255
Asp700I	GAANNNNTTC	3 cut(s)	1917, 2949, 2979
AspA2I	CCTAGG	1 cut(s)	1166
AsuC2I	CCSGG	2 cut(s)	200, 410
AvaI	CYCGRG	1 cut(s)	3026
AvaII	GGWCC	6 cut(s)	270, 305, 1179, 1958, 2527, 2656
AvrII	CCTAGG	1 cut(s)	1166
BaeGI	GKGCMC	1 cut(s)	3027
BalI	TGGCCA	2 cut(s)	321, 3449
BanI	GGYRCC	2 cut(s)	299, 1012
BanII	GRGCYC	1 cut(s)	3027
BauI	CACGAG	2 cut(s)	2089, 2832
BbsI	GAAGAC	1 cut(s)	1292
Bbv12I	GWGCWC	1 cut(s)	2640
BbvCI	CCTCAGC	1 cut(s)	2310
BceAI	ACGGC	7 cut(s)	119, 539, 555, 676, 1556, 2445, 3398
BcgI	CGANNNNNNTGC	4 cut(s)	557, 591, 2375, 2409
BciT130I	CCWGG	4 cut(s)	1339, 1362, 1688, 2900
BciVI	GTATCC	1 cut(s)	374
BclI	TGATCA	4 cut(s)	396, 759, 3342, 3367
BcnI	CCSGG	2 cut(s)	200, 410
BcoDI	GTCTC	5 cut(s)	821, 1050, 1801, 2922, 3220
BfaI	CTAG	5 cut(s)	782, 1167, 1296, 1692, 3242
BfmI	CTRYAG	3 cut(s)	1857, 2479, 3453
BfoI	RGCGCY	6 cut(s)	213, 236, 303, 505, 545, 1016
BfuI	GTATCC	1 cut(s)	374
BlnI	CCTAGG	1 cut(s)	1166
Bme1390I	CCNGG	6 cut(s)	200, 410, 1339, 1362, 1688, 2900
Bme18I	GGWCC	6 cut(s)	270, 305, 1179, 1958, 2527, 2656
BmeT110I	CYCGRG	1 cut(s)	3026
BmiI	GGNNCC	8 cut(s)	301, 307, 610, 1014, 1643, 2529, 2657, 3025
BmrFI	CCNGG	6 cut(s)	200, 410, 1339, 1362, 1688, 2900
BmrI	ACTGGG	3 cut(s)	305, 1002, 1522
BmsI	GCATC	7 cut(s)	1202, 2293, 2383, 2557, 2578, 3021, 3349
BmuI	ACTGGG	3 cut(s)	305, 1002, 1522
BpiI	GAAGAC	1 cut(s)	1292
BplI	GAGNNNNNCTC	2 cut(s)	3385, 3417
BpmI	CTGGAG	3 cut(s)	638, 1812, 2201
Bpu10I	CCTNAGC	2 cut(s)	2310, 3389
BpuEI	CTTGAG	5 cut(s)	336, 935, 1640, 2840, 3160
BpuMI	CCSGG	2 cut(s)	200, 410
BsaAI	YACGTR	1 cut(s)	1851
BsaBI	GATNNNNATC	1 cut(s)	3341
BsaHI	GRCGYC	4 cut(s)	300, 387, 467, 1013
BsaI	GGTCTC	1 cut(s)	821
BsaWI	WCCGGW	1 cut(s)	444
BsaXI	ACNNNNNCTCC	2 cut(s)	2199, 2229
Bse118I	RCCGGY	3 cut(s)	302, 769, 1291
Bse1I	ACTGG	8 cut(s)	300, 997, 1210, 1512, 1517, 3046, 3331, 3419
Bse3DI	GCAATG	4 cut(s)	7, 1065, 1590, 3075
Bse8I	GATNNNNATC	1 cut(s)	3341
BseBI	CCWGG	4 cut(s)	1339, 1362, 1688, 2900
BseGI	GGATG	9 cut(s)	1193, 1487, 1602, 1723, 2009, 2697, 2875, 3364, 3402
BseJI	GATNNNNATC	1 cut(s)	3341
BseMI	GCAATG	4 cut(s)	7, 1065, 1590, 3075
BseMII	CTCAG	4 cut(s)	160, 881, 1613, 2301
BseNI	ACTGG	8 cut(s)	300, 997, 1210, 1512, 1517, 3046, 3331, 3419
BseRI	GAGGAG	2 cut(s)	506, 833
BseSI	GKGCMC	1 cut(s)	3027
BseX3I	CGGCCG	1 cut(s)	2458
BseYI	CCCAGC	3 cut(s)	259, 2651, 2701
BsgI	GTGCAG	3 cut(s)	1133, 2195, 2940
Bsh1236I	CGCG	1 cut(s)	1177
Bsh1285I	CGRYCG	3 cut(s)	445, 2098, 2461
BshNI	GGYRCC	2 cut(s)	299, 1012
BsiEI	CGRYCG	3 cut(s)	445, 2098, 2461
BsiHKAI	GWGCWC	1 cut(s)	2640
BsiHKCI	CYCGRG	1 cut(s)	3026
BsiSI	CCGG	8 cut(s)	200, 303, 410, 445, 470, 770, 1292, 3438
BslFI	GGGAC	8 cut(s)	291, 361, 802, 941, 1532, 1535, 2834, 3241
BsmAI	GTCTC	5 cut(s)	821, 1050, 1801, 2922, 3220
BsmFI	GGGAC	8 cut(s)	291, 361, 802, 941, 1532, 1535, 2834, 3241
BsmI	GAATGC	1 cut(s)	725
Bso31I	GGTCTC	1 cut(s)	821
BsoBI	CYCGRG	1 cut(s)	3026
Bsp120I	GGGCCC	1 cut(s)	3023
Bsp1286I	GDGCHC	2 cut(s)	2640, 3027
Bsp19I	CCATGG	2 cut(s)	525, 2466
BspCNI	CTCAG	4 cut(s)	159, 880, 1614, 2302
BspFNI	CGCG	1 cut(s)	1177
BspHI	TCATGA	4 cut(s)	420, 921, 1615, 2923
BspLI	GGNNCC	8 cut(s)	301, 307, 610, 1014, 1643, 2529, 2657, 3025
BspMAI	CTGCAG	2 cut(s)	1861, 3457
BspPI	GGATC	3 cut(s)	686, 1266, 2174
BspQI	GCTCTTC	1 cut(s)	3507
BspT107I	GGYRCC	2 cut(s)	299, 1012
BspTNI	GGTCTC	1 cut(s)	821
BsrBI	CCGCTC	2 cut(s)	156, 573
BsrDI	GCAATG	4 cut(s)	7, 1065, 1590, 3075
BsrFI	RCCGGY	3 cut(s)	302, 769, 1291
BsrI	ACTGG	8 cut(s)	300, 997, 1210, 1512, 1517, 3046, 3331, 3419
BssAI	RCCGGY	3 cut(s)	302, 769, 1291
BssNI	GRCGYC	4 cut(s)	300, 387, 467, 1013
BssSI	CACGAG	2 cut(s)	2089, 2832
BssT1I	CCWWGG	7 cut(s)	525, 1166, 2466, 2862, 3001, 3087, 3199
Bst2BI	CACGAG	2 cut(s)	2089, 2832
Bst2UI	CCWGG	4 cut(s)	1339, 1362, 1688, 2900
Bst4CI	ACNGT	8 cut(s)	1009, 2071, 2099, 2122, 2780, 2848, 3133, 3433
Bst6I	CTCTTC	5 cut(s)	46, 1799, 1897, 2925, 3507
BstACI	GRCGYC	4 cut(s)	300, 387, 467, 1013
BstBAI	YACGTR	1 cut(s)	1851
BstDEI	CTNAG	6 cut(s)	146, 867, 1052, 1622, 2310, 3389
BstDSI	CCRYGG	4 cut(s)	525, 1008, 2466, 3261
BstENI	CCTNNNNNAGG	1 cut(s)	494
BstF5I	GGATG	9 cut(s)	1193, 1487, 1602, 1723, 2009, 2697, 2875, 3364, 3402
BstFNI	CGCG	1 cut(s)	1177
BstH2I	RGCGCY	6 cut(s)	213, 236, 303, 505, 545, 1016
BstMAI	GTCTC	5 cut(s)	821, 1050, 1801, 2922, 3220
BstMCI	CGRYCG	3 cut(s)	445, 2098, 2461
BstNI	CCWGG	4 cut(s)	1339, 1362, 1688, 2900
BstNSI	RCATGY	2 cut(s)	2270, 2963
BstSCI	CCNGG	6 cut(s)	198, 408, 1337, 1360, 1686, 2898
BstSFI	CTRYAG	3 cut(s)	1857, 2479, 3453
BstSLI	GKGCMC	1 cut(s)	3027
BstUI	CGCG	1 cut(s)	1177
BstV2I	GAAGAC	1 cut(s)	1292
BstZI	CGGCCG	1 cut(s)	2458
BsuI	GTATCC	1 cut(s)	374
BtgI	CCRYGG	4 cut(s)	525, 1008, 2466, 3261
BtsCI	GGATG	9 cut(s)	1193, 1487, 1602, 1723, 2009, 2697, 2875, 3364, 3402
BtsI	GCAGTG	2 cut(s)	30, 3450
BtsIMutI	CAGTG	6 cut(s)	30, 1756, 2114, 3039, 3129, 3450
CaiI	CAGNNNCTG	1 cut(s)	259
CciI	TCATGA	4 cut(s)	420, 921, 1615, 2923
Cfr10I	RCCGGY	3 cut(s)	302, 769, 1291
CseI	GACGC	3 cut(s)	475, 518, 712
Csp6I	GTAC	5 cut(s)	1425, 1888, 2283, 2844, 3014
CviQI	GTAC	5 cut(s)	1425, 1888, 2283, 2844, 3014
DdeI	CTNAG	6 cut(s)	146, 867, 1052, 1622, 2310, 3389
DinI	GGCGCC	2 cut(s)	301, 1014
EaeI	YGGCCR	6 cut(s)	319, 411, 438, 2458, 2745, 3447
EagI	CGGCCG	1 cut(s)	2458
Eam1104I	CTCTTC	5 cut(s)	46, 1799, 1897, 2925, 3507
EarI	CTCTTC	5 cut(s)	46, 1799, 1897, 2925, 3507
EclXI	CGGCCG	1 cut(s)	2458
Eco130I	CCWWGG	7 cut(s)	525, 1166, 2466, 2862, 3001, 3087, 3199
Eco147I	AGGCCT	1 cut(s)	3198
Eco24I	GRGCYC	1 cut(s)	3027
Eco31I	GGTCTC	1 cut(s)	821
Eco47I	GGWCC	6 cut(s)	270, 305, 1179, 1958, 2527, 2656
Eco52I	CGGCCG	1 cut(s)	2458
Eco57I	CTGAAG	7 cut(s)	299, 1732, 2006, 2219, 3207, 3296, 3488
Eco88I	CYCGRG	1 cut(s)	3026
EcoNI	CCTNNNNNAGG	1 cut(s)	494
EcoRI	GAATTC	1 cut(s)	2053
EcoRII	CCWGG	4 cut(s)	1337, 1360, 1686, 2898
EcoT14I	CCWWGG	7 cut(s)	525, 1166, 2466, 2862, 3001, 3087, 3199
EcoT38I	GRGCYC	1 cut(s)	3027
EgeI	GGCGCC	2 cut(s)	301, 1014
EheI	GGCGCC	2 cut(s)	301, 1014
ErhI	CCWWGG	7 cut(s)	525, 1166, 2466, 2862, 3001, 3087, 3199
FalI	AAGNNNNNCTT	2 cut(s)	2967, 2999
FaqI	GGGAC	8 cut(s)	291, 361, 802, 941, 1532, 1535, 2834, 3241
FauI	CCCGC	2 cut(s)	1833, 2759
FbaI	TGATCA	4 cut(s)	396, 759, 3342, 3367
FokI	GGATG	9 cut(s)	1180, 1494, 1609, 1730, 1996, 2684, 2862, 3371, 3409
FriOI	GRGCYC	1 cut(s)	3027
FspAI	RTGCGCAY	1 cut(s)	2273
FspBI	CTAG	5 cut(s)	782, 1167, 1296, 1692, 3242
FspI	TGCGCA	2 cut(s)	1020, 2273
GsaI	CCCAGC	3 cut(s)	263, 2655, 2705
GsuI	CTGGAG	3 cut(s)	638, 1812, 2201
HaeII	RGCGCY	6 cut(s)	213, 236, 303, 505, 545, 1016
HapII	CCGG	8 cut(s)	200, 303, 410, 445, 470, 770, 1292, 3438
HgaI	GACGC	3 cut(s)	475, 518, 712
Hin1I	GRCGYC	4 cut(s)	300, 387, 467, 1013
HindIII	AAGCTT	1 cut(s)	354
HpaII	CCGG	8 cut(s)	200, 303, 410, 445, 470, 770, 1292, 3438
Hpy166II	GTNNAC	2 cut(s)	1367, 2527
Hpy8I	GTNNAC	2 cut(s)	1367, 2527
Hpy99I	CGWCG	4 cut(s)	389, 392, 512, 728
HpyCH4III	ACNGT	8 cut(s)	1009, 2071, 2099, 2122, 2780, 2848, 3133, 3433
HpyCH4IV	ACGT	2 cut(s)	387, 1850
HpyF3I	CTNAG	6 cut(s)	146, 867, 1052, 1622, 2310, 3389
HpySE526I	ACGT	2 cut(s)	387, 1850
Hsp92I	GRCGYC	4 cut(s)	300, 387, 467, 1013
KasI	GGCGCC	2 cut(s)	299, 1012
KroI	GCCGGC	1 cut(s)	769
KroNI	GCCGGC	1 cut(s)	771
Ksp22I	TGATCA	4 cut(s)	396, 759, 3342, 3367
LguI	GCTCTTC	1 cut(s)	3507
LweI	GCATC	7 cut(s)	1202, 2293, 2383, 2557, 2578, 3021, 3349
MaeI	CTAG	5 cut(s)	782, 1167, 1296, 1692, 3242
MaeII	ACGT	2 cut(s)	387, 1850
MaeIII	GTNAC	6 cut(s)	1324, 1751, 1822, 2497, 2666, 3305
MbiI	CCGCTC	2 cut(s)	156, 573
MfeI	CAATTG	2 cut(s)	2403, 2868
MhlI	GDGCHC	2 cut(s)	2640, 3027
MlsI	TGGCCA	2 cut(s)	321, 3449
MluNI	TGGCCA	2 cut(s)	321, 3449
Mly113I	GGCGCC	2 cut(s)	300, 1013
MlyI	GAGTC	9 cut(s)	144, 343, 1415, 1445, 1554, 2288, 2594, 2663, 3478
MmeI	TCCRAC	5 cut(s)	84, 429, 1946, 2461, 2711
Mox20I	TGGCCA	2 cut(s)	321, 3449
MroNI	GCCGGC	1 cut(s)	769
MroXI	GAANNNNTTC	3 cut(s)	1917, 2949, 2979
MscI	TGGCCA	2 cut(s)	321, 3449
MseI	TTAA	9 cut(s)	1500, 2328, 2340, 2348, 2931, 3146, 3155, 3207, 3489
MslI	CAYNNNNRTG	4 cut(s)	419, 2610, 2664, 3491
Msp20I	TGGCCA	2 cut(s)	321, 3449
MspA1I	CMGCKG	2 cut(s)	259, 1048
MspI	CCGG	8 cut(s)	200, 303, 410, 445, 470, 770, 1292, 3438
MspR9I	CCNGG	6 cut(s)	200, 410, 1339, 1362, 1688, 2900
MunI	CAATTG	2 cut(s)	2403, 2868
Mva1269I	GAATGC	1 cut(s)	725
MvaI	CCWGG	4 cut(s)	1339, 1362, 1688, 2900
MvnI	CGCG	1 cut(s)	1177
NaeI	GCCGGC	1 cut(s)	771
NarI	GGCGCC	2 cut(s)	300, 1013
NciI	CCSGG	2 cut(s)	200, 410
NcoI	CCATGG	2 cut(s)	525, 2466
NgoMIV	GCCGGC	1 cut(s)	769
NlaIV	GGNNCC	8 cut(s)	301, 307, 610, 1014, 1643, 2529, 2657, 3025
NmeAIII	GCCGAG	5 cut(s)	204, 592, 1163, 2268, 2773
NmuCI	GTSAC	4 cut(s)	1751, 2497, 2666, 3305
NsbI	TGCGCA	2 cut(s)	1020, 2273
NspI	RCATGY	2 cut(s)	2270, 2963
PagI	TCATGA	4 cut(s)	420, 921, 1615, 2923
PceI	AGGCCT	1 cut(s)	3198
PciI	ACATGT	2 cut(s)	2266, 2959
PciSI	GCTCTTC	1 cut(s)	3507
PcsI	WCGNNNNNNNCGW	1 cut(s)	2839
PctI	GAATGC	1 cut(s)	725
PdiI	GCCGGC	1 cut(s)	771
PdmI	GAANNNNTTC	3 cut(s)	1917, 2949, 2979
PfeI	GAWTC	9 cut(s)	19, 286, 998, 1126, 1162, 1333, 1563, 1913, 3415
PflMI	CCANNNNNTGG	2 cut(s)	3125, 3337
PleI	GAGTC	9 cut(s)	144, 343, 1414, 1444, 1553, 2288, 2594, 2663, 3477
PluTI	GGCGCC	2 cut(s)	303, 1016
PpsI	GAGTC	9 cut(s)	144, 343, 1414, 1444, 1553, 2288, 2594, 2663, 3477
Ppu21I	YACGTR	1 cut(s)	1851
PscI	ACATGT	2 cut(s)	2266, 2959
PsiI	TTATAA	1 cut(s)	810
Psp6I	CCWGG	4 cut(s)	1337, 1360, 1686, 2898
PspFI	CCCAGC	3 cut(s)	259, 2651, 2701
PspGI	CCWGG	4 cut(s)	1337, 1360, 1686, 2898
PspN4I	GGNNCC	8 cut(s)	301, 307, 610, 1014, 1643, 2529, 2657, 3025
PspOMI	GGGCCC	1 cut(s)	3023
PstI	CTGCAG	2 cut(s)	1861, 3457
PstNI	CAGNNNCTG	1 cut(s)	259
PvuII	CAGCTG	1 cut(s)	259
RsaI	GTAC	5 cut(s)	1426, 1889, 2284, 2845, 3015
RsaNI	GTAC	5 cut(s)	1425, 1888, 2283, 2844, 3014
RseI	CAYNNNNRTG	4 cut(s)	419, 2610, 2664, 3491
SapI	GCTCTTC	1 cut(s)	3507
SaqAI	TTAA	9 cut(s)	1500, 2328, 2340, 2348, 2931, 3146, 3155, 3207, 3489
SchI	GAGTC	9 cut(s)	144, 343, 1415, 1445, 1554, 2288, 2594, 2663, 3478
ScrFI	CCNGG	6 cut(s)	200, 410, 1339, 1362, 1688, 2900
SduI	GDGCHC	2 cut(s)	2640, 3027
SfaNI	GCATC	7 cut(s)	1202, 2293, 2383, 2557, 2578, 3021, 3349
SfcI	CTRYAG	3 cut(s)	1857, 2479, 3453
SfoI	GGCGCC	2 cut(s)	301, 1014
SinI	GGWCC	6 cut(s)	270, 305, 1179, 1958, 2527, 2656
SmiMI	CAYNNNNRTG	4 cut(s)	419, 2610, 2664, 3491
SmlI	CTYRAG	5 cut(s)	351, 914, 1655, 2819, 3139
SmoI	CTYRAG	5 cut(s)	351, 914, 1655, 2819, 3139
SseBI	AGGCCT	1 cut(s)	3198
SspDI	GGCGCC	2 cut(s)	299, 1012
SspI	AATATT	2 cut(s)	970, 1377
SspMI	CTAG	5 cut(s)	782, 1167, 1296, 1692, 3242
StuI	AGGCCT	1 cut(s)	3198
StyD4I	CCNGG	6 cut(s)	198, 408, 1337, 1360, 1686, 2898
StyI	CCWWGG	7 cut(s)	525, 1166, 2466, 2862, 3001, 3087, 3199
TaaI	ACNGT	8 cut(s)	1009, 2071, 2099, 2122, 2780, 2848, 3133, 3433
TaiI	ACGT	2 cut(s)	390, 1853
TaqI	TCGA	6 cut(s)	336, 492, 726, 1456, 1561, 1966
TaqII	GACCGA	2 cut(s)	287, 440
TatI	WGTACW	2 cut(s)	1887, 3013
TauI	GCSGC	6 cut(s)	134, 238, 691, 854, 1051, 2460
TfiI	GAWTC	9 cut(s)	19, 286, 998, 1126, 1162, 1333, 1563, 1913, 3415
Tru1I	TTAA	9 cut(s)	1500, 2328, 2340, 2348, 2931, 3146, 3155, 3207, 3489
Tru9I	TTAA	9 cut(s)	1500, 2328, 2340, 2348, 2931, 3146, 3155, 3207, 3489
TscAI	CASTG	6 cut(s)	37, 1756, 2121, 3046, 3136, 3457
TseFI	GTSAC	4 cut(s)	1751, 2497, 2666, 3305
Tsp45I	GTSAC	4 cut(s)	1751, 2497, 2666, 3305
TspRI	CASTG	6 cut(s)	37, 1756, 2121, 3046, 3136, 3457
Van91I	CCANNNNNTGG	2 cut(s)	3125, 3337
VpaK11BI	GGWCC	6 cut(s)	270, 305, 1179, 1958, 2527, 2656
XagI	CCTNNNNNAGG	1 cut(s)	494
XapI	RAATTY	6 cut(s)	1263, 1355, 1611, 1985, 2053, 3255
XbaI	TCTAGA	1 cut(s)	3241
XceI	RCATGY	2 cut(s)	2270, 2963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1318
XmaJI	CCTAGG	1 cut(s)	1166
XmnI	GAANNNNTTC	3 cut(s)	1917, 2949, 2979
XspI	CTAG	5 cut(s)	782, 1167, 1296, 1692, 3242
ZraI	GACGTC	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.