Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

42346043 .. 42348037

Gene Structure 1995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG331800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGGGTAGATACGTGTGCCGCAAGTTTGAGGCCAATACTCCTTATATGTACTCGTCTTATGACTTTGAGTGTGAATCCGCTCCCACCCAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAGACAATCATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACAAGTGACCGTTTATATTTTGAGCCCCTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTTGGAGGTCAAACACCTCTGAAGCTAGCACTTCCCCTCCAGCGGTATTTAGATGAAAACAAGCCTAGATGTGCAAGTGGTGCTGGACATGTTCGCATTTGGGGTACCACACCTGACTCCATAGATGCTGCTGAGGACAGGGAAAAGTTCAATGCAATTCTGAACGAGTTAAAGATTGAACAGCCAAAAGGAGGAATTGCCAAGAGTGAAGCAGATGCTCTTGCCATTGCCACAAACATTGGCTACCCTGTCGTTGTCCGACCCTCCTATGTATTGGGCGGCCGTGCCATGGAGATAGTATATAGTGATGAAAAACTTGTGACCTACCTAGAAACTGCTGTTGAGGTGGACCCGGAACGTCCTGTATTGATTGACAGATATCTATCCGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTCGTCATTGGTGGGATAATGGAGCACATCGAGCAGGCTGGGGTCCATTCTGGTGATTCTGCTTGCTCGATTCCGACAAAAACCATCCCAGAGTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCTAAGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGAGATGTTTATTTACTCGAGGCAAATCCTCGTGCTTCCCGTACGGTCCCTTTTGTGTCCAAGGCAATTGGACATCCGTTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGATCTTGGTTTCACAAAAGAAGTCATTCCTGCACATATGTCAGTGAAAGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTATTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCAATTGCATTTGCCAAGGCTCAGATTGCTGCTGGGCAGGCGCTACCACTTTCAGGCACTGTGTTTTTGAGCTTAAATGACTTGACCAAACCCCATCTTGAGAGAATTGCAAAGGCATTTTTAGGCCTTGGATTTAAGATTGTTTCAACTTCAGGAACAGCTCATGTTCTTGAACTAGCGAAAATTCCAGTGGAACGAGTGCTGAAGCTACATGAGGGGAGGCCTAATGCTGGTGACACGGTTGCTAATGGGCAAATCCAGTTGATGGTGATTACAAGTTCTGGTGATGCACTCGATCAGATTGATGGACGGCAGCTAAGGAGGACGGCCCTTGCTTACAAGATTCCAGTAATAACAACAGTTGCTGGAGCTTTGGCAACTGCAGAAGCAATAAAGAGCTTAAAGACCAGCTCCATTAAGATGATTGCACTTCAGGACTTCTTTGATGACGAGAATAAAGCTGTCACTGATAAAAAGTTGCAGTCTTCCTCTGTCTCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

578

Amino Acids

62.87

Weight (kDa)

5.46

Isoelectric Point (pI)

35.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	30 - 155	3e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	159 - 362	1.3e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	167 - 333	2.2e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	186 - 331	9.5e-08	D-ala D-ala ligase C-terminus
MGS	PF02142	445 - 530	1.5e-19	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	443
AccB1I	GGYRCC	1 cut(s)	443
AccB7I	CCANNNNNTGG	1 cut(s)	1498
AccBSI	CCGCTC	1 cut(s)	80
AciI	CCGC	4 cut(s)	19, 78, 382, 618
AclWI	GGATC	1 cut(s)	335
AcoI	YGGCCR	1 cut(s)	619
AcsI	RAATTY	3 cut(s)	146, 214, 1416
AcuI	CTGAAG	5 cut(s)	167, 380, 1368, 1457, 1649
AfaI	GTAC	3 cut(s)	50, 445, 1006
AfiI	CCNNNNNNNGG	6 cut(s)	381, 530, 628, 1286, 1463, 1498
AflIII	ACRYGT	2 cut(s)	12, 427
AgsI	TTSAA	8 cut(s)	146, 219, 287, 490, 518, 877, 1380, 1406
AjnI	CCWGG	1 cut(s)	1059
Alw21I	GWGCWC	1 cut(s)	801
Alw26I	GTCTC	3 cut(s)	194, 1083, 1732
AlwI	GGATC	1 cut(s)	335
AlwNI	CAGNNNCTG	2 cut(s)	1292, 1598
Ama87I	CYCGRG	1 cut(s)	980
AoxI	GGCC	6 cut(s)	30, 619, 1184, 1357, 1454, 1560
ApeKI	GCWGC	3 cut(s)	467, 1262, 1546
ApoI	RAATTY	3 cut(s)	146, 214, 1416
Asp700I	GAANNNNTTC	2 cut(s)	1110, 1140
Asp718I	GGTACC	1 cut(s)	443
AspLEI	GCGC	1 cut(s)	1276
AspS9I	GGNCC	6 cut(s)	119, 688, 817, 1009, 1184, 1561
AsuC2I	CCSGG	1 cut(s)	692
AsuHPI	GGTGA	6 cut(s)	839, 1217, 1222, 1478, 1513, 1529
AsuNHI	GCTAGC	1 cut(s)	364
AvaI	CYCGRG	1 cut(s)	980
AvaII	GGWCC	4 cut(s)	119, 688, 817, 1009
BanI	GGYRCC	1 cut(s)	443
BanII	GRGCYC	1 cut(s)	276
BauI	CACGAG	1 cut(s)	993
BbsI	GAAGAC	1 cut(s)	1710
Bbv12I	GWGCWC	1 cut(s)	801
BbvCI	CCTCAGC	1 cut(s)	471
BbvI	GCAGC	3 cut(s)	454, 1249, 1558
BccI	CCATC	6 cut(s)	317, 866, 1207, 1335, 1492, 1532
BceAI	ACGGC	3 cut(s)	606, 1559, 1575
BciT130I	CCWGG	1 cut(s)	1061
BcnI	CCSGG	1 cut(s)	692
BcoDI	GTCTC	3 cut(s)	194, 1083, 1732
BfaI	CTAG	4 cut(s)	365, 405, 668, 1409
BfmI	CTRYAG	1 cut(s)	1614
BfoI	RGCGCY	1 cut(s)	1277
BisI	GCNGC	5 cut(s)	19, 468, 619, 1263, 1547
BlsI	GCNGC	5 cut(s)	20, 469, 620, 1264, 1548
Bme1390I	CCNGG	2 cut(s)	692, 1061
Bme18I	GGWCC	4 cut(s)	119, 688, 817, 1009
BmeT110I	CYCGRG	1 cut(s)	980
BmgT120I	GGNCC	6 cut(s)	119, 688, 817, 1009, 1184, 1561
BmiI	GGNNCC	4 cut(s)	445, 690, 818, 1011
BmrFI	CCNGG	2 cut(s)	692, 1061
BmsI	GCATC	6 cut(s)	454, 544, 718, 739, 1182, 1510
BmtI	GCTAGC	1 cut(s)	368
BpiI	GAAGAC	1 cut(s)	1710
BpmI	CTGGAG	3 cut(s)	362, 984, 1620
Bpu10I	CCTNAGC	2 cut(s)	471, 1550
BpuEI	CTTGAG	1 cut(s)	1352
BpuMI	CCSGG	1 cut(s)	692
BsaAI	YACGTR	1 cut(s)	13
BsaBI	GATNNNNATC	1 cut(s)	721
BsaJI	CCNNGG	6 cut(s)	627, 1023, 1059, 1162, 1248, 1360
BsaXI	ACNNNNNCTCC	2 cut(s)	360, 390
Bsc4I	CCNNNNNNNGG	6 cut(s)	381, 530, 628, 1286, 1463, 1498
Bse1I	ACTGG	4 cut(s)	1207, 1421, 1492, 1580
Bse3DI	GCAATG	1 cut(s)	564
Bse8I	GATNNNNATC	1 cut(s)	721
BseBI	CCWGG	1 cut(s)	1061
BseDI	CCNNGG	6 cut(s)	627, 1023, 1059, 1162, 1248, 1360
BseGI	GGATG	3 cut(s)	170, 858, 1036
BseJI	GATNNNNATC	1 cut(s)	721
BseLI	CCNNNNNNNGG	6 cut(s)	381, 530, 628, 1286, 1463, 1498
BseMI	GCAATG	1 cut(s)	564
BseMII	CTCAG	3 cut(s)	462, 1179, 1268
BseNI	ACTGG	4 cut(s)	1207, 1421, 1492, 1580
BseX3I	CGGCCG	1 cut(s)	619
BseXI	GCAGC	3 cut(s)	454, 1249, 1558
BseYI	CCCAGC	2 cut(s)	812, 1265
BsgI	GTGCAG	2 cut(s)	356, 1101
Bsh1285I	CGRYCG	1 cut(s)	622
BshFI	GGCC	6 cut(s)	32, 621, 1186, 1359, 1456, 1562
BshNI	GGYRCC	1 cut(s)	443
BsiEI	CGRYCG	1 cut(s)	622
BsiHKAI	GWGCWC	1 cut(s)	801
BsiHKCI	CYCGRG	1 cut(s)	980
BsiSI	CCGG	1 cut(s)	692
BsiWI	CGTACG	1 cut(s)	1004
BslFI	GGGAC	1 cut(s)	995
BslI	CCNNNNNNNGG	6 cut(s)	381, 530, 628, 1286, 1463, 1498
BsmAI	GTCTC	3 cut(s)	194, 1083, 1732
BsmFI	GGGAC	1 cut(s)	995
BsnI	GGCC	6 cut(s)	32, 621, 1186, 1359, 1456, 1562
BsoBI	CYCGRG	1 cut(s)	980
Bsp1286I	GDGCHC	2 cut(s)	276, 801
Bsp143I	GATC	3 cut(s)	327, 1087, 1528
Bsp19I	CCATGG	1 cut(s)	627
BspACI	CCGC	4 cut(s)	19, 78, 382, 618
BspANI	GGCC	6 cut(s)	32, 621, 1186, 1359, 1456, 1562
BspCNI	CTCAG	3 cut(s)	463, 1180, 1267
BspHI	TCATGA	2 cut(s)	207, 1084
BspLI	GGNNCC	4 cut(s)	445, 690, 818, 1011
BspMAI	CTGCAG	1 cut(s)	1618
BspOI	GCTAGC	1 cut(s)	368
BspPI	GGATC	1 cut(s)	335
BspT107I	GGYRCC	1 cut(s)	443
BsrBI	CCGCTC	1 cut(s)	80
BsrDI	GCAATG	1 cut(s)	564
BsrI	ACTGG	4 cut(s)	1207, 1421, 1492, 1580
BssECI	CCNNGG	6 cut(s)	627, 1023, 1059, 1162, 1248, 1360
BssMI	GATC	3 cut(s)	327, 1087, 1528
BssSI	CACGAG	1 cut(s)	993
BssT1I	CCWWGG	5 cut(s)	627, 1023, 1162, 1248, 1360
Bst2BI	CACGAG	1 cut(s)	993
Bst2UI	CCWGG	1 cut(s)	1061
Bst4CI	ACNGT	8 cut(s)	232, 260, 283, 941, 1009, 1294, 1474, 1594
Bst6I	CTCTTC	1 cut(s)	1086
BstAPI	GCANNNNNTGC	1 cut(s)	419
BstBAI	YACGTR	1 cut(s)	13
BstC8I	GCNNGC	4 cut(s)	366, 810, 838, 1272
BstDEI	CTNAG	5 cut(s)	471, 909, 1188, 1254, 1550
BstDSI	CCRYGG	1 cut(s)	627
BstF5I	GGATG	3 cut(s)	170, 858, 1036
BstH2I	RGCGCY	1 cut(s)	1277
BstHHI	GCGC	1 cut(s)	1276
BstKTI	GATC	3 cut(s)	330, 1090, 1531
BstMAI	GTCTC	3 cut(s)	194, 1083, 1732
BstMBI	GATC	3 cut(s)	327, 1087, 1528
BstMCI	CGRYCG	1 cut(s)	622
BstMWI	GCNNNNNNNGC	6 cut(s)	419, 805, 1052, 1192, 1259, 1271
BstNI	CCWGG	1 cut(s)	1061
BstNSI	RCATGY	1 cut(s)	431
BstSCI	CCNGG	2 cut(s)	690, 1059
BstSFI	CTRYAG	1 cut(s)	1614
BstV1I	GCAGC	3 cut(s)	454, 1249, 1558
BstV2I	GAAGAC	1 cut(s)	1710
BstZI	CGGCCG	1 cut(s)	619
BsuRI	GGCC	6 cut(s)	32, 621, 1186, 1359, 1456, 1562
BtgI	CCRYGG	1 cut(s)	627
BtsCI	GGATG	3 cut(s)	170, 858, 1036
BtsIMutI	CAGTG	5 cut(s)	1134, 1200, 1290, 1428, 1698
Cac8I	GCNNGC	4 cut(s)	366, 810, 838, 1272
CaiI	CAGNNNCTG	2 cut(s)	1292, 1598
CciI	TCATGA	2 cut(s)	207, 1084
CfoI	GCGC	1 cut(s)	1276
Cfr13I	GGNCC	6 cut(s)	119, 688, 817, 1009, 1184, 1561
Csp6I	GTAC	3 cut(s)	49, 444, 1005
CspCI	CAANNNNNGTGG	2 cut(s)	559, 594
CviAII	CATG	8 cut(s)	208, 428, 628, 767, 891, 1085, 1397, 1445
CviQI	GTAC	3 cut(s)	49, 444, 1005
DdeI	CTNAG	5 cut(s)	471, 909, 1188, 1254, 1550
DpnI	GATC	3 cut(s)	329, 1089, 1530
DpnII	GATC	3 cut(s)	327, 1087, 1528
EaeI	YGGCCR	1 cut(s)	619
EagI	CGGCCG	1 cut(s)	619
Eam1104I	CTCTTC	1 cut(s)	1086
EarI	CTCTTC	1 cut(s)	1086
EclXI	CGGCCG	1 cut(s)	619
Eco130I	CCWWGG	5 cut(s)	627, 1023, 1162, 1248, 1360
Eco147I	AGGCCT	2 cut(s)	1359, 1456
Eco24I	GRGCYC	1 cut(s)	276
Eco32I	GATATC	1 cut(s)	719
Eco47I	GGWCC	4 cut(s)	119, 688, 817, 1009
Eco52I	CGGCCG	1 cut(s)	619
Eco57I	CTGAAG	5 cut(s)	167, 380, 1368, 1457, 1649
Eco88I	CYCGRG	1 cut(s)	980
EcoRI	GAATTC	1 cut(s)	214
EcoRII	CCWGG	1 cut(s)	1059
EcoRV	GATATC	1 cut(s)	719
EcoT14I	CCWWGG	5 cut(s)	627, 1023, 1162, 1248, 1360
EcoT38I	GRGCYC	1 cut(s)	276
ErhI	CCWWGG	5 cut(s)	627, 1023, 1162, 1248, 1360
FaeI	CATG	8 cut(s)	211, 431, 631, 770, 894, 1088, 1400, 1448
FalI	AAGNNNNNCTT	2 cut(s)	1128, 1160
FaqI	GGGAC	1 cut(s)	995
FatI	CATG	8 cut(s)	207, 427, 627, 766, 890, 1084, 1396, 1444
FauNDI	CATATG	1 cut(s)	1122
Fnu4HI	GCNGC	5 cut(s)	19, 468, 619, 1263, 1547
FokI	GGATG	3 cut(s)	157, 845, 1023
FriOI	GRGCYC	1 cut(s)	276
Fsp4HI	GCNGC	5 cut(s)	19, 468, 619, 1263, 1547
FspBI	CTAG	4 cut(s)	365, 405, 668, 1409
GlaI	GCGC	1 cut(s)	1275
GluI	GCNGC	5 cut(s)	19, 468, 619, 1263, 1547
GsaI	CCCAGC	2 cut(s)	816, 1269
GsuI	CTGGAG	3 cut(s)	362, 984, 1620
HaeII	RGCGCY	1 cut(s)	1277
HaeIII	GGCC	6 cut(s)	32, 621, 1186, 1359, 1456, 1562
HapII	CCGG	1 cut(s)	692
HhaI	GCGC	1 cut(s)	1276
Hin1II	CATG	8 cut(s)	211, 431, 631, 770, 894, 1088, 1400, 1448
Hin6I	GCGC	1 cut(s)	1274
HinP1I	GCGC	1 cut(s)	1274
HinfI	GANTC	7 cut(s)	74, 455, 761, 830, 844, 866, 1576
HpaII	CCGG	1 cut(s)	692
HphI	GGTGA	6 cut(s)	839, 1217, 1222, 1478, 1513, 1529
Hpy166II	GTNNAC	1 cut(s)	688
Hpy188I	TCNGA	8 cut(s)	186, 360, 501, 599, 727, 849, 1257, 1533
Hpy188III	TCNNGA	8 cut(s)	208, 224, 963, 1085, 1331, 1386, 1403, 1667
Hpy8I	GTNNAC	1 cut(s)	688
HpyAV	CCTTC	2 cut(s)	91, 191
HpyCH4III	ACNGT	8 cut(s)	232, 260, 283, 941, 1009, 1294, 1474, 1594
HpyCH4IV	ACGT	2 cut(s)	12, 697
HpyF10VI	GCNNNNNNNGC	6 cut(s)	419, 805, 1052, 1192, 1259, 1271
HpyF3I	CTNAG	5 cut(s)	471, 909, 1188, 1254, 1550
HpySE526I	ACGT	2 cut(s)	12, 697
Hsp92II	CATG	8 cut(s)	211, 431, 631, 770, 894, 1088, 1400, 1448
HspAI	GCGC	1 cut(s)	1274
KpnI	GGTACC	1 cut(s)	447
Kzo9I	GATC	3 cut(s)	327, 1087, 1528
LmnI	GCTCC	4 cut(s)	85, 796, 1601, 1649
Lsp1109I	GCAGC	3 cut(s)	454, 1249, 1558
LweI	GCATC	6 cut(s)	454, 544, 718, 739, 1182, 1510
MaeI	CTAG	4 cut(s)	365, 405, 668, 1409
MaeII	ACGT	2 cut(s)	12, 697
MaeIII	GTNAC	4 cut(s)	254, 658, 1466, 1696
MalI	GATC	3 cut(s)	329, 1089, 1530
MbiI	CCGCTC	1 cut(s)	80
MboI	GATC	3 cut(s)	327, 1087, 1528
MboII	GAAGA	4 cut(s)	299, 1073, 1136, 1710
MfeI	CAATTG	2 cut(s)	1029, 1236
MhlI	GDGCHC	2 cut(s)	276, 801
MlyI	GAGTC	3 cut(s)	449, 755, 875
MmeI	TCCRAC	2 cut(s)	622, 872
MroXI	GAANNNNTTC	2 cut(s)	1110, 1140
MseI	TTAA	5 cut(s)	509, 1307, 1368, 1634, 1650
MslI	CAYNNNNRTG	3 cut(s)	771, 825, 1652
MspA1I	CMGCKG	1 cut(s)	382
MspI	CCGG	1 cut(s)	692
MspR9I	CCNGG	2 cut(s)	692, 1061
MunI	CAATTG	2 cut(s)	1029, 1236
MvaI	CCWGG	1 cut(s)	1061
MwoI	GCNNNNNNNGC	6 cut(s)	419, 805, 1052, 1192, 1259, 1271
NciI	CCSGG	1 cut(s)	692
NcoI	CCATGG	1 cut(s)	627
NdeI	CATATG	1 cut(s)	1122
NdeII	GATC	3 cut(s)	327, 1087, 1528
NheI	GCTAGC	1 cut(s)	364
NlaIII	CATG	8 cut(s)	211, 431, 631, 770, 894, 1088, 1400, 1448
NlaIV	GGNNCC	4 cut(s)	445, 690, 818, 1011
NmuCI	GTSAC	4 cut(s)	254, 658, 1466, 1696
NspI	RCATGY	1 cut(s)	431
PaeR7I	CTCGAG	1 cut(s)	980
PagI	TCATGA	2 cut(s)	207, 1084
PceI	AGGCCT	2 cut(s)	1359, 1456
PciI	ACATGT	1 cut(s)	427
PcsI	WCGNNNNNNNCGW	1 cut(s)	1000
PdmI	GAANNNNTTC	2 cut(s)	1110, 1140
PfeI	GAWTC	4 cut(s)	74, 830, 844, 1576
Pfl23II	CGTACG	1 cut(s)	1004
PflMI	CCANNNNNTGG	1 cut(s)	1498
PkrI	GCNGC	5 cut(s)	20, 469, 620, 1264, 1548
PleI	GAGTC	3 cut(s)	449, 755, 874
PpsI	GAGTC	3 cut(s)	449, 755, 874
Ppu21I	YACGTR	1 cut(s)	13
PscI	ACATGT	1 cut(s)	427
Psp6I	CCWGG	1 cut(s)	1059
PspFI	CCCAGC	2 cut(s)	812, 1265
PspGI	CCWGG	1 cut(s)	1059
PspLI	CGTACG	1 cut(s)	1004
PspN4I	GGNNCC	4 cut(s)	445, 690, 818, 1011
PspPI	GGNCC	6 cut(s)	119, 688, 817, 1009, 1184, 1561
PspXI	VCTCGAGB	1 cut(s)	980
PstI	CTGCAG	1 cut(s)	1618
PstNI	CAGNNNCTG	2 cut(s)	1292, 1598
RsaI	GTAC	3 cut(s)	50, 445, 1006
RsaNI	GTAC	3 cut(s)	49, 444, 1005
RseI	CAYNNNNRTG	3 cut(s)	771, 825, 1652
SaqAI	TTAA	5 cut(s)	509, 1307, 1368, 1634, 1650
SatI	GCNGC	5 cut(s)	19, 468, 619, 1263, 1547
Sau3AI	GATC	3 cut(s)	327, 1087, 1528
Sau96I	GGNCC	6 cut(s)	119, 688, 817, 1009, 1184, 1561
SchI	GAGTC	3 cut(s)	449, 755, 875
ScrFI	CCNGG	2 cut(s)	692, 1061
SduI	GDGCHC	2 cut(s)	276, 801
SfaNI	GCATC	6 cut(s)	454, 544, 718, 739, 1182, 1510
SfcI	CTRYAG	1 cut(s)	1614
Sfr274I	CTCGAG	1 cut(s)	980
SinI	GGWCC	4 cut(s)	119, 688, 817, 1009
SlaI	CTCGAG	1 cut(s)	980
SmiMI	CAYNNNNRTG	3 cut(s)	771, 825, 1652
SmlI	CTYRAG	2 cut(s)	980, 1331
SmoI	CTYRAG	2 cut(s)	980, 1331
SseBI	AGGCCT	2 cut(s)	1359, 1456
SsiI	CCGC	4 cut(s)	19, 78, 382, 618
SspMI	CTAG	4 cut(s)	365, 405, 668, 1409
StuI	AGGCCT	2 cut(s)	1359, 1456
StyD4I	CCNGG	2 cut(s)	690, 1059
StyI	CCWWGG	5 cut(s)	627, 1023, 1162, 1248, 1360
TaaI	ACNGT	8 cut(s)	232, 260, 283, 941, 1009, 1294, 1474, 1594
TaiI	ACGT	2 cut(s)	15, 700
TaqI	TCGA	5 cut(s)	127, 804, 842, 981, 1527
TatI	WGTACW	1 cut(s)	48
TauI	GCSGC	2 cut(s)	21, 621
TfiI	GAWTC	4 cut(s)	74, 830, 844, 1576
Tru1I	TTAA	5 cut(s)	509, 1307, 1368, 1634, 1650
Tru9I	TTAA	5 cut(s)	509, 1307, 1368, 1634, 1650
TscAI	CASTG	5 cut(s)	1134, 1207, 1297, 1428, 1705
TseFI	GTSAC	4 cut(s)	254, 658, 1466, 1696
TseI	GCWGC	3 cut(s)	467, 1262, 1546
Tsp45I	GTSAC	4 cut(s)	254, 658, 1466, 1696
TspDTI	ATGAA	5 cut(s)	227, 408, 663, 950, 1073
TspGWI	ACGGA	1 cut(s)	1029
TspRI	CASTG	5 cut(s)	1134, 1207, 1297, 1428, 1705
Van91I	CCANNNNNTGG	1 cut(s)	1498
VpaK11BI	GGWCC	4 cut(s)	119, 688, 817, 1009
XapI	RAATTY	3 cut(s)	146, 214, 1416
XceI	RCATGY	1 cut(s)	431
XhoI	CTCGAG	1 cut(s)	980
XmnI	GAANNNNTTC	2 cut(s)	1110, 1140
XspI	CTAG	4 cut(s)	365, 405, 668, 1409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.