Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

17506390 .. 17511009

Gene Structure 4620 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g25660.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGGCCATTGCATGAATGAGATTCTGGCTTTGAAGCAGCCGCCGATTTTGGCTCAGGCGAAACCCGCCTATCCGTCGAAGCCGAACCATCTCCGCTTCTTCCTCTACTCGAAGAAGCTCGGCGCGTCACCGTCGCTCAGCCTCCGGTCATGGCCGGCGACGGCGAAGGCTCGTCCGAGTTTCCTAGCCAACTCGGTTCGGAGCGAGTCTCTGTCCAACGGCGCGGCGGCGAAGAAAGAAGAGGCGGCGGCGGCGGAGAAGAAGGTGGGGAAGAGGACGGACTTGAAGAAGATTATGATACTGGGCGCCGGGCCGATTGTGATCGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAGGCGTGTAAGGCGTTGAAGGATGATGGATACGAGGTCGTTTTGATCAATTCCAACCCGGCTACCATCATGACCGACCCGGATTTTGCTGATAGGACCTACATTACGCCGATGACGCCGGAATTGGTGGAGCAGGTGCTGGAGAAGGAGCGGCCCGACGCGCTTTTGCCGACGATGGGAGGCCAGACGGCATTGAATTTGGCGGTGGCGCTGGCCGAGAGCGGTGCGCTGGAGAAGTATGGTGTGGAGTTGATTGGAGCTAAGCTGGAGGCGATTAAGAAAGCAGAGGATAGAGAGCTGTTCAAGGAGGCCATGAAGAACATTGGGATTAAAACGCCGCCGTCTGGAGTTGCCAACACCTTGGAGGAATGCATCGAAATTGCGAAAGAGATTGGTGAGTTTCCCTTGATTATTCGGCCGGCTTTTACTTTAGGAGGGACAGGAGGTGGAATTGCATATAATAAGGAGGAGTTCGAGACAATATGTAAGGCGGGGCTGGCGGCGAGTACAAACTCTCAGGTTTTGGTGGAGAAGTCATTATTGGGGTGGAAGGAGTATGAGCTTGAGGTGATGAGAGACTTGGCAGACAATGTGGTTATTATTTGTTCAATTGAGAATATCGACCCGATGGGAGTTCATACCGGGGACTCTATCACTGTGGCGCCTGCTCAAACTTTGACTGATAAGGAGTATCAGCGGCTTAGGGACTATTCGATAGCTATAATAAGGGAGATTGGAGTGGAATGCGGTGGTTCTAATGTGCAGTTTGCAGTGAATCCTCAAGACGGGGAAGTTATGGTGATTGAGATGAATCCGAGGGTGTCAAGGTCCTCAGCTCTGGCGTCAAAAGCTACTGGTTTTCCGATAGCAAAAATGGCTGCCAAGTTGTCAGTTGGGTATTCATTGGATCAAATTCCCAATGACATTACCAAGAAGACACCGGCTAGTTTTGAACCATCCATAGACTATGTGGTAACAAAGATCCCCCGGTTTGCTTTTGAAAAGTTTCCTGGTTCAGAGCCAATACTGACAACGCAGATGAAATCTGTCGGCGAGTCAATGGCATTGGGGCGTACATTTCAAGAATCCTTTCAGAAAGCAGTTAGGTCTTTGGAATGCGGTTTCTCTGGGTGGGGTTGTGGGAAAATTAAGGAACTAGACTGGGATTGGGACCAGTTGAAATATAGCCTCCGAGTTCCTAACCCAGAGCGTATCCATGCAGTGTATGCTGCAATGAAGAAGGGAATGAAGGTAGATGAGATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACTCAGCTTAAGGAATTGGTGGATGTAGAGCAATTTCTCCTGGCCAGAACTATATCTGATTTAACAAAGGATGATTTTTATGAAGTGAAGAGAAGAGGCTTCAGTGACAAACAAATAGCTTTTGCCATCAAATCCTCTGAAAATGAAGTGCGGTTGAAGAGATTATCATTGGGTGTCACTCCAGCATATAAGCGGGTAGATACATGTGCCGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCGTCTTATGACTTTGAGTGTGAATCAGCTCCCACCCAAGAAAAGAAGGTTTTAATTTTAGGTGGAGGACCAAATCGAATCGGTCAGGGTATTGAGTTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCGGAAAGCAGGATATGAGACAATTATGATGAACTCAAATCCTGAAACAGTATCCACTGATTATGACACGAGTGACCGTTTATATTTTGAACCCCTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGCATCATTGTTCAGTTTGGAGGTCAAACACCACTGAAGCTAGCACTTCCCATCCAGCGGTATTTGGATGAAAACAAGCCTAGATGTGCAAGTGGGACTGGACATGTTTGCATTTGGGGTACAACACCTGACTCCATAGATGCTGCCGAGGACAGGGAGAAGTTCAATGCTATTCTGAATGAGTTGAAGATTGAACAGCCAGAAGGAGGAATTGCCAAGAGTGAAGCAGATGCTCTTGCCATTGCCAAAAATATCGGCTACCCTGTGGTTGTCCGGCCCTCCTATGTGTTGGGCGGCCGTGCCATGGAGATAGTATATAGTGATGAAAAACTTGTGACCTACCTTGAAACTGCTGTTGAGGTGGACCCGGAACGCCCAGTATTGATTGACAGGTATTTGTCAGATGCCATTGAAATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATCGAGCAGGCTGGTGTCCATTCTGGTGATTCTGCTTGCTCACTTCCAACAAAAACCATCCCAGAATCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCCAAGAGGTTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACTATGTCAGGAGATGTTTATTTACTCGAGGCAAATCCTCGTGCTTCCCGTACGATCCCTTTTGTGTCTAAGGCAATTGGACATCCATTGGCTAAATATGCTTCGCTGGTAATGTCCGGAAAGTCTCTTCATGACCTTGGTTTCACAAAAGAAGTCATTCCCGCACATATGTCAGTGAAAGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTATTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTATGAGTTCCCCATTGCATTTGCAAAGGCTCAGATTGCTGCTGGCCAGGCACCACCACTTTCAGGCACTGTGTTTTTGAGCTTAAATGACTTGACCAAATCCCATCTTGAGAGGATTGCAAAGGCATTTTTGGGCCTTGGATTCAAGATTGTTTCAACTTCAGGAACAGCTCGTGTTCTTGAATTAGCGAAAATTCCGGTGGAACGAGTGCTGAAGCTACATGAGGGGCGGCCTAACGCTGGTGACATGGTTGCTAATGGGCAAATCCAGTTGATGGTGATTACTAGTTCTGGTGATGCACTCGATCAGATTGATGGGCGACAGCTAAGGAGGACGGCCCTTGCTTACAAGATTCCAGTAATAACAACAGTTGCCGGAGCTTTGGCAACCGCAGAAGCAATAAAGAGCTTAAAGTCCAGCTCCATTAAGATGATTGCACTTCAGGACTTCTTTGATGACGAAAATAAAGCTGCAAGTGACAAAAAGTTGCAGCCTTCCTCTACCTCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1194

Amino Acids

130.31

Weight (kDa)

5.46

Isoelectric Point (pI)

37.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	95 - 212	3.2e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	216 - 423	7.1e-73	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	515 - 592	9.1e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	646 - 769	4.6e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	774 - 977	3.5e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	782 - 948	6.8e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	801 - 946	4.4e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1060 - 1145	1.1e-19	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	481
Acc36I	ACCTGC	1 cut(s)	481
AccB1I	GGYRCC	3 cut(s)	305, 1018, 3118
AccB7I	CCANNNNNTGG	1 cut(s)	3343
AccBSI	CCGCTC	2 cut(s)	508, 579
AccII	CGCG	3 cut(s)	125, 224, 518
AccIII	TCCGGA	1 cut(s)	2915
AclWI	GGATC	3 cut(s)	1272, 1333, 2848
AcoI	YGGCCR	7 cut(s)	152, 570, 773, 1695, 2464, 2751, 3112
AcsI	RAATTY	3 cut(s)	553, 1269, 3261
AcuI	CTGAAG	5 cut(s)	1738, 2225, 3213, 3302, 3494
AcyI	GRCGYC	4 cut(s)	306, 473, 1019, 1199
AfaI	GTAC	5 cut(s)	865, 1432, 1895, 2290, 2851
AflII	CTTAAG	1 cut(s)	1661
AflIII	ACRYGT	2 cut(s)	1856, 2272
AhlI	ACTAGT	1 cut(s)	3353
AjnI	CCWGG	3 cut(s)	1366, 1692, 3114
AjuI	GAANNNNNNNTTGG	2 cut(s)	881, 913
Alw21I	GWGCWC	1 cut(s)	2646
Alw26I	GTCTC	5 cut(s)	213, 827, 927, 2039, 2928
AlwI	GGATC	3 cut(s)	1272, 1333, 2848
AlwNI	CAGNNNCTG	2 cut(s)	496, 3137
Ama87I	CYCGRG	2 cut(s)	349, 2825
Aor13HI	TCCGGA	1 cut(s)	2915
ApeKI	GCWGC	7 cut(s)	37, 1235, 1586, 2312, 3107, 3539, 3559
ApoI	RAATTY	3 cut(s)	553, 1269, 3261
ArsI	GACNNNNNNTTYG	2 cut(s)	425, 457
Asp700I	GAANNNNTTC	4 cut(s)	1362, 1446, 2955, 2985
AspLEI	GCGC	7 cut(s)	125, 224, 308, 520, 568, 586, 1021
AsuC2I	CCSGG	6 cut(s)	310, 416, 437, 1000, 1345, 2537
AsuNHI	GCTAGC	1 cut(s)	2209
AvaI	CYCGRG	2 cut(s)	349, 2825
AvaII	GGWCC	5 cut(s)	453, 1185, 1528, 1964, 2533
BalI	TGGCCA	3 cut(s)	1697, 2753, 3114
BanI	GGYRCC	3 cut(s)	305, 1018, 3118
BauI	CACGAG	3 cut(s)	2095, 2838, 3240
BbsI	GAAGAC	1 cut(s)	1298
Bbv12I	GWGCWC	1 cut(s)	2646
BbvCI	CCTCAGC	1 cut(s)	1189
BbvI	GCAGC	7 cut(s)	49, 1222, 1573, 2299, 3094, 3526, 3571
BceAI	ACGGC	6 cut(s)	177, 235, 561, 682, 2451, 3420
BcgI	CGANNNNNNTGC	2 cut(s)	563, 597
BciT130I	CCWGG	3 cut(s)	1368, 1694, 3116
BciVI	GTATCC	3 cut(s)	380, 1580, 2089
BclI	TGATCA	1 cut(s)	402
BcnI	CCSGG	6 cut(s)	310, 416, 437, 1000, 1345, 2537
BcoDI	GTCTC	5 cut(s)	213, 827, 927, 2039, 2928
BcuI	ACTAGT	1 cut(s)	3353
BfaI	CTAG	6 cut(s)	185, 1302, 1514, 2210, 2250, 3354
BfoI	RGCGCY	3 cut(s)	309, 569, 1022
BfrI	CTTAAG	1 cut(s)	1661
BfuAI	ACCTGC	1 cut(s)	481
BfuI	GTATCC	3 cut(s)	380, 1580, 2089
BlpI	GCTNAGC	2 cut(s)	137, 618
Bme1390I	CCNGG	9 cut(s)	310, 416, 437, 1000, 1345, 1368, 1694, 2537, 3116
Bme18I	GGWCC	5 cut(s)	453, 1185, 1528, 1964, 2533
BmeT110I	CYCGRG	2 cut(s)	349, 2825
BmiI	GGNNCC	6 cut(s)	307, 1020, 1529, 1649, 2535, 3120
BmrFI	CCNGG	9 cut(s)	310, 416, 437, 1000, 1345, 1368, 1694, 2537, 3116
BmrI	ACTGGG	3 cut(s)	311, 1528, 2540
BmsI	GCATC	8 cut(s)	738, 2181, 2299, 2389, 2563, 2584, 3027, 3355
BmtI	GCTAGC	1 cut(s)	2213
BmuI	ACTGGG	3 cut(s)	311, 1528, 2540
BpiI	GAAGAC	1 cut(s)	1298
BplI	GAGNNNNNCTC	2 cut(s)	193, 225
BpmI	CTGGAG	5 cut(s)	518, 608, 644, 723, 1818
Bpu10I	CCTNAGC	4 cut(s)	54, 1058, 1189, 3395
Bpu1102I	GCTNAGC	2 cut(s)	137, 618
BpuEI	CTTGAG	3 cut(s)	941, 1122, 3197
BpuMI	CCSGG	6 cut(s)	310, 416, 437, 1000, 1345, 2537
BsaBI	GATNNNNATC	1 cut(s)	320
BsaHI	GRCGYC	4 cut(s)	306, 473, 1019, 1199
BsaJI	CCNNGG	9 cut(s)	717, 999, 1172, 1343, 2316, 2472, 2935, 3007, 3205
BsaWI	WCCGGW	3 cut(s)	144, 2915, 3265
Bse118I	RCCGGY	3 cut(s)	154, 775, 1297
Bse1I	ACTGG	9 cut(s)	306, 1216, 1523, 1531, 2272, 2546, 3052, 3337, 3425
Bse3DI	GCAATG	4 cut(s)	7, 1596, 2409, 3081
Bse8I	GATNNNNATC	1 cut(s)	320
BseAI	TCCGGA	1 cut(s)	2915
BseBI	CCWGG	3 cut(s)	1368, 1694, 3116
BseDI	CCNNGG	9 cut(s)	717, 999, 1172, 1343, 2316, 2472, 2935, 3007, 3205
BseGI	GGATG	9 cut(s)	385, 1313, 1681, 1729, 2015, 2220, 2242, 2703, 2881
BseJI	GATNNNNATC	1 cut(s)	320
BseMI	GCAATG	4 cut(s)	7, 1596, 2409, 3081
BseMII	CTCAG	9 cut(s)	68, 151, 371, 887, 1203, 1619, 1670, 3024, 3113
BseNI	ACTGG	9 cut(s)	306, 1216, 1523, 1531, 2272, 2546, 3052, 3337, 3425
BseRI	GAGGAG	1 cut(s)	839
BseX3I	CGGCCG	2 cut(s)	773, 2464
BseXI	GCAGC	7 cut(s)	49, 1222, 1573, 2299, 3094, 3526, 3571
BsgI	GTGCAG	1 cut(s)	1139
Bsh1236I	CGCG	3 cut(s)	125, 224, 518
Bsh1285I	CGRYCG	2 cut(s)	776, 2467
BshNI	GGYRCC	3 cut(s)	305, 1018, 3118
BsiEI	CGRYCG	2 cut(s)	776, 2467
BsiHKAI	GWGCWC	1 cut(s)	2646
BsiHKCI	CYCGRG	2 cut(s)	349, 2825
BsiWI	CGTACG	1 cut(s)	2849
BslFI	GGGAC	6 cut(s)	367, 808, 1016, 1076, 1541, 2278
BsmAI	GTCTC	5 cut(s)	213, 827, 927, 2039, 2928
BsmFI	GGGAC	6 cut(s)	367, 808, 1016, 1076, 1541, 2278
BsmI	GAATGC	3 cut(s)	731, 1106, 1478
BsoBI	CYCGRG	2 cut(s)	349, 2825
Bsp1286I	GDGCHC	1 cut(s)	2646
Bsp13I	TCCGGA	1 cut(s)	2915
Bsp143I	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
Bsp1720I	GCTNAGC	2 cut(s)	137, 618
Bsp19I	CCATGG	1 cut(s)	2472
BspCNI	CTCAG	9 cut(s)	67, 150, 370, 886, 1202, 1620, 1669, 3025, 3112
BspEI	TCCGGA	1 cut(s)	2915
BspFNI	CGCG	3 cut(s)	125, 224, 518
BspHI	TCATGA	3 cut(s)	426, 1621, 2929
BspLI	GGNNCC	6 cut(s)	307, 1020, 1529, 1649, 2535, 3120
BspMI	ACCTGC	1 cut(s)	481
BspOI	GCTAGC	1 cut(s)	2213
BspPI	GGATC	3 cut(s)	1272, 1333, 2848
BspT107I	GGYRCC	3 cut(s)	305, 1018, 3118
BspTI	CTTAAG	1 cut(s)	1661
BsrBI	CCGCTC	2 cut(s)	508, 579
BsrDI	GCAATG	4 cut(s)	7, 1596, 2409, 3081
BsrFI	RCCGGY	3 cut(s)	154, 775, 1297
BsrI	ACTGG	9 cut(s)	306, 1216, 1523, 1531, 2272, 2546, 3052, 3337, 3425
BssAI	RCCGGY	3 cut(s)	154, 775, 1297
BssECI	CCNNGG	9 cut(s)	717, 999, 1172, 1343, 2316, 2472, 2935, 3007, 3205
BssMI	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
BssNI	GRCGYC	4 cut(s)	306, 473, 1019, 1199
BssSI	CACGAG	3 cut(s)	2095, 2838, 3240
BssT1I	CCWWGG	5 cut(s)	717, 2472, 2935, 3007, 3205
Bst2BI	CACGAG	3 cut(s)	2095, 2838, 3240
Bst2UI	CCWGG	3 cut(s)	1368, 1694, 3116
Bst4CI	ACNGT	8 cut(s)	132, 1015, 2077, 2105, 2128, 2786, 3139, 3439
Bst6I	CTCTTC	6 cut(s)	234, 266, 1736, 1741, 1805, 2931
BstACI	GRCGYC	4 cut(s)	306, 473, 1019, 1199
BstAFI	CTTAAG	1 cut(s)	1661
BstAPI	GCANNNNNTGC	1 cut(s)	1583
BstDSI	CCRYGG	1 cut(s)	2472
BstF5I	GGATG	9 cut(s)	385, 1313, 1681, 1729, 2015, 2220, 2242, 2703, 2881
BstFNI	CGCG	3 cut(s)	125, 224, 518
BstH2I	RGCGCY	3 cut(s)	309, 569, 1022
BstHHI	GCGC	7 cut(s)	125, 224, 308, 520, 568, 586, 1021
BstKTI	GATC	6 cut(s)	324, 405, 1267, 1341, 2856, 3376
BstMAI	GTCTC	5 cut(s)	213, 827, 927, 2039, 2928
BstMBI	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
BstMCI	CGRYCG	2 cut(s)	776, 2467
BstNI	CCWGG	3 cut(s)	1368, 1694, 3116
BstNSI	RCATGY	2 cut(s)	1860, 2276
BstSCI	CCNGG	9 cut(s)	308, 414, 435, 998, 1343, 1366, 1692, 2535, 3114
BstUI	CGCG	3 cut(s)	125, 224, 518
BstV1I	GCAGC	7 cut(s)	49, 1222, 1573, 2299, 3094, 3526, 3571
BstV2I	GAAGAC	1 cut(s)	1298
BstX2I	RGATCY	1 cut(s)	1338
BstXI	CCANNNNNNTGG	1 cut(s)	718
BstYI	RGATCY	1 cut(s)	1338
BstZI	CGGCCG	2 cut(s)	773, 2464
BsuI	GTATCC	3 cut(s)	380, 1580, 2089
BtgI	CCRYGG	1 cut(s)	2472
BtsCI	GGATG	9 cut(s)	385, 1313, 1681, 1729, 2015, 2220, 2242, 2703, 2881
BtsI	GCAGTG	2 cut(s)	1134, 1584
BtsIMutI	CAGTG	9 cut(s)	1011, 1134, 1584, 1762, 2082, 2201, 2979, 3045, 3135
BveI	ACCTGC	1 cut(s)	481
CaiI	CAGNNNCTG	2 cut(s)	496, 3137
CciI	TCATGA	3 cut(s)	426, 1621, 2929
CfoI	GCGC	7 cut(s)	125, 224, 308, 520, 568, 586, 1021
Cfr10I	RCCGGY	3 cut(s)	154, 775, 1297
CseI	GACGC	4 cut(s)	114, 481, 524, 1188
Csp6I	GTAC	5 cut(s)	864, 1431, 1894, 2289, 2850
CviQI	GTAC	5 cut(s)	864, 1431, 1894, 2289, 2850
DinI	GGCGCC	2 cut(s)	307, 1020
DpnI	GATC	6 cut(s)	323, 404, 1266, 1340, 2855, 3375
DpnII	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
EaeI	YGGCCR	7 cut(s)	152, 570, 773, 1695, 2464, 2751, 3112
EagI	CGGCCG	2 cut(s)	773, 2464
Eam1104I	CTCTTC	6 cut(s)	234, 266, 1736, 1741, 1805, 2931
EarI	CTCTTC	6 cut(s)	234, 266, 1736, 1741, 1805, 2931
EciI	GGCGGA	1 cut(s)	269
EclXI	CGGCCG	2 cut(s)	773, 2464
Eco130I	CCWWGG	5 cut(s)	717, 2472, 2935, 3007, 3205
Eco47I	GGWCC	5 cut(s)	453, 1185, 1528, 1964, 2533
Eco52I	CGGCCG	2 cut(s)	773, 2464
Eco57I	CTGAAG	5 cut(s)	1738, 2225, 3213, 3302, 3494
Eco88I	CYCGRG	2 cut(s)	349, 2825
EcoO109I	RGGNCCY	2 cut(s)	453, 1185
EcoRII	CCWGG	3 cut(s)	1366, 1692, 3114
EcoT14I	CCWWGG	5 cut(s)	717, 2472, 2935, 3007, 3205
EcoT22I	ATGCAT	1 cut(s)	731
EgeI	GGCGCC	2 cut(s)	307, 1020
EheI	GGCGCC	2 cut(s)	307, 1020
ErhI	CCWWGG	5 cut(s)	717, 2472, 2935, 3007, 3205
FalI	AAGNNNNNCTT	2 cut(s)	2973, 3005
FaqI	GGGAC	6 cut(s)	367, 808, 1016, 1076, 1541, 2278
FauI	CCCGC	4 cut(s)	73, 841, 1839, 2968
FauNDI	CATATG	1 cut(s)	2967
FbaI	TGATCA	1 cut(s)	402
FokI	GGATG	9 cut(s)	392, 1300, 1688, 1736, 2002, 2207, 2249, 2690, 2868
FspBI	CTAG	6 cut(s)	185, 1302, 1514, 2210, 2250, 3354
GlaI	GCGC	7 cut(s)	124, 223, 307, 519, 567, 585, 1020
GsuI	CTGGAG	5 cut(s)	518, 608, 644, 723, 1818
HaeII	RGCGCY	3 cut(s)	309, 569, 1022
HgaI	GACGC	4 cut(s)	114, 481, 524, 1188
HhaI	GCGC	7 cut(s)	125, 224, 308, 520, 568, 586, 1021
Hin1I	GRCGYC	4 cut(s)	306, 473, 1019, 1199
Hin6I	GCGC	7 cut(s)	123, 222, 306, 518, 566, 584, 1019
HinP1I	GCGC	7 cut(s)	123, 222, 306, 518, 566, 584, 1019
HindIII	AAGCTT	1 cut(s)	330
Hpy166II	GTNNAC	1 cut(s)	2533
Hpy8I	GTNNAC	1 cut(s)	2533
Hpy99I	CGWCG	5 cut(s)	79, 136, 163, 518, 532
HpyCH4III	ACNGT	8 cut(s)	132, 1015, 2077, 2105, 2128, 2786, 3139, 3439
Hsp92I	GRCGYC	4 cut(s)	306, 473, 1019, 1199
HspAI	GCGC	7 cut(s)	123, 222, 306, 518, 566, 584, 1019
KasI	GGCGCC	2 cut(s)	305, 1018
Kpn2I	TCCGGA	1 cut(s)	2915
KroI	GCCGGC	2 cut(s)	154, 775
KroNI	GCCGGC	2 cut(s)	156, 777
Ksp22I	TGATCA	1 cut(s)	402
Kzo9I	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
LmnI	GCTCC	8 cut(s)	201, 487, 505, 614, 1930, 2641, 3446, 3494
Lsp1109I	GCAGC	7 cut(s)	49, 1222, 1573, 2299, 3094, 3526, 3571
LweI	GCATC	8 cut(s)	738, 2181, 2299, 2389, 2563, 2584, 3027, 3355
MaeI	CTAG	6 cut(s)	185, 1302, 1514, 2210, 2250, 3354
MaeIII	GTNAC	8 cut(s)	126, 1330, 1757, 1828, 2099, 2503, 3311, 3545
MalI	GATC	6 cut(s)	323, 404, 1266, 1340, 2855, 3375
MbiI	CCGCTC	2 cut(s)	508, 579
MboI	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
MfeI	CAATTG	2 cut(s)	966, 2874
MflI	RGATCY	1 cut(s)	1338
MhlI	GDGCHC	1 cut(s)	2646
MlsI	TGGCCA	3 cut(s)	1697, 2753, 3114
MluNI	TGGCCA	3 cut(s)	1697, 2753, 3114
Mly113I	GGCGCC	2 cut(s)	306, 1019
MlyI	GAGTC	6 cut(s)	215, 349, 998, 1421, 2294, 2600
MmeI	TCCRAC	3 cut(s)	240, 435, 2717
Mox20I	TGGCCA	3 cut(s)	1697, 2753, 3114
Mph1103I	ATGCAT	1 cut(s)	731
MroI	TCCGGA	1 cut(s)	2915
MroNI	GCCGGC	2 cut(s)	154, 775
MroXI	GAANNNNTTC	4 cut(s)	1362, 1446, 2955, 2985
MscI	TGGCCA	3 cut(s)	1697, 2753, 3114
MseI	TTAA	9 cut(s)	633, 687, 1506, 1662, 1715, 1949, 3152, 3479, 3495
MslI	CAYNNNNRTG	3 cut(s)	2616, 2670, 3497
Msp20I	TGGCCA	3 cut(s)	1697, 2753, 3114
MspA1I	CMGCKG	2 cut(s)	1054, 2227
MspCI	CTTAAG	1 cut(s)	1661
MspR9I	CCNGG	9 cut(s)	310, 416, 437, 1000, 1345, 1368, 1694, 2537, 3116
MunI	CAATTG	2 cut(s)	966, 2874
Mva1269I	GAATGC	3 cut(s)	731, 1106, 1478
MvaI	CCWGG	3 cut(s)	1368, 1694, 3116
MvnI	CGCG	3 cut(s)	125, 224, 518
NaeI	GCCGGC	2 cut(s)	156, 777
NarI	GGCGCC	2 cut(s)	306, 1019
NciI	CCSGG	6 cut(s)	310, 416, 437, 1000, 1345, 2537
NcoI	CCATGG	1 cut(s)	2472
NdeI	CATATG	1 cut(s)	2967
NdeII	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
NgoMIV	GCCGGC	2 cut(s)	154, 775
NheI	GCTAGC	1 cut(s)	2209
NlaIV	GGNNCC	6 cut(s)	307, 1020, 1529, 1649, 2535, 3120
NmeAIII	GCCGAG	3 cut(s)	99, 598, 2341
NmuCI	GTSAC	7 cut(s)	126, 1757, 1828, 2099, 2503, 3311, 3545
NsiI	ATGCAT	1 cut(s)	731
NspI	RCATGY	2 cut(s)	1860, 2276
PaeR7I	CTCGAG	1 cut(s)	2825
PagI	TCATGA	3 cut(s)	426, 1621, 2929
PaqCI	CACCTGC	1 cut(s)	481
PciI	ACATGT	2 cut(s)	1856, 2272
PcsI	WCGNNNNNNNCGW	1 cut(s)	2845
PctI	GAATGC	3 cut(s)	731, 1106, 1478
PdiI	GCCGGC	2 cut(s)	156, 777
PdmI	GAANNNNTTC	4 cut(s)	1362, 1446, 2955, 2985
Pfl23II	CGTACG	1 cut(s)	2849
PflMI	CCANNNNNTGG	1 cut(s)	3343
PleI	GAGTC	6 cut(s)	214, 349, 998, 1420, 2294, 2600
PluTI	GGCGCC	2 cut(s)	309, 1022
PpsI	GAGTC	6 cut(s)	214, 349, 998, 1420, 2294, 2600
PpuMI	RGGWCCY	2 cut(s)	453, 1185
PscI	ACATGT	2 cut(s)	1856, 2272
Psp5II	RGGWCCY	2 cut(s)	453, 1185
Psp6I	CCWGG	3 cut(s)	1366, 1692, 3114
PspGI	CCWGG	3 cut(s)	1366, 1692, 3114
PspLI	CGTACG	1 cut(s)	2849
PspN4I	GGNNCC	6 cut(s)	307, 1020, 1529, 1649, 2535, 3120
PspPPI	RGGWCCY	2 cut(s)	453, 1185
PspXI	VCTCGAGB	1 cut(s)	2825
PstNI	CAGNNNCTG	2 cut(s)	496, 3137
PsuI	RGATCY	1 cut(s)	1338
RsaI	GTAC	5 cut(s)	865, 1432, 1895, 2290, 2851
RsaNI	GTAC	5 cut(s)	864, 1431, 1894, 2289, 2850
RseI	CAYNNNNRTG	3 cut(s)	2616, 2670, 3497
SaqAI	TTAA	9 cut(s)	633, 687, 1506, 1662, 1715, 1949, 3152, 3479, 3495
Sau3AI	GATC	6 cut(s)	321, 402, 1264, 1338, 2853, 3373
SchI	GAGTC	6 cut(s)	215, 349, 998, 1421, 2294, 2600
ScrFI	CCNGG	9 cut(s)	310, 416, 437, 1000, 1345, 1368, 1694, 2537, 3116
SduI	GDGCHC	1 cut(s)	2646
SfaNI	GCATC	8 cut(s)	738, 2181, 2299, 2389, 2563, 2584, 3027, 3355
SfoI	GGCGCC	2 cut(s)	307, 1020
Sfr274I	CTCGAG	1 cut(s)	2825
SinI	GGWCC	5 cut(s)	453, 1185, 1528, 1964, 2533
SlaI	CTCGAG	1 cut(s)	2825
SmiMI	CAYNNNNRTG	3 cut(s)	2616, 2670, 3497
SmlI	CTYRAG	5 cut(s)	920, 1137, 1661, 2825, 3176
SmoI	CTYRAG	5 cut(s)	920, 1137, 1661, 2825, 3176
SpeI	ACTAGT	1 cut(s)	3353
SspDI	GGCGCC	2 cut(s)	305, 1018
SspMI	CTAG	6 cut(s)	185, 1302, 1514, 2210, 2250, 3354
StyD4I	CCNGG	9 cut(s)	308, 414, 435, 998, 1343, 1366, 1692, 2535, 3114
StyI	CCWWGG	5 cut(s)	717, 2472, 2935, 3007, 3205
TaaI	ACNGT	8 cut(s)	132, 1015, 2077, 2105, 2128, 2786, 3139, 3439
TaqII	GACCGA	2 cut(s)	446, 1967
TatI	WGTACW	2 cut(s)	863, 1893
Tru1I	TTAA	9 cut(s)	633, 687, 1506, 1662, 1715, 1949, 3152, 3479, 3495
Tru9I	TTAA	9 cut(s)	633, 687, 1506, 1662, 1715, 1949, 3152, 3479, 3495
TscAI	CASTG	9 cut(s)	1018, 1134, 1584, 1762, 2089, 2208, 2979, 3052, 3142
TseFI	GTSAC	7 cut(s)	126, 1757, 1828, 2099, 2503, 3311, 3545
TseI	GCWGC	7 cut(s)	37, 1235, 1586, 2312, 3107, 3539, 3559
Tsp45I	GTSAC	7 cut(s)	126, 1757, 1828, 2099, 2503, 3311, 3545
TspGWI	ACGGA	2 cut(s)	63, 293
TspRI	CASTG	9 cut(s)	1018, 1134, 1584, 1762, 2089, 2208, 2979, 3052, 3142
Van91I	CCANNNNNTGG	1 cut(s)	3343
Vha464I	CTTAAG	1 cut(s)	1661
VpaK11BI	GGWCC	5 cut(s)	453, 1185, 1528, 1964, 2533
XapI	RAATTY	3 cut(s)	553, 1269, 3261
XceI	RCATGY	2 cut(s)	1860, 2276
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1324
XhoI	CTCGAG	1 cut(s)	2825
XmnI	GAANNNNTTC	4 cut(s)	1362, 1446, 2955, 2985
XspI	CTAG	6 cut(s)	185, 1302, 1514, 2210, 2250, 3354
Zsp2I	ATGCAT	1 cut(s)	731

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.