Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Reverse (-)

30617685 .. 30623428

Gene Structure 5744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG316400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3567 bp

ATGGGCCATTGCTGCATGAATCACACCGAGACTCCTCTCTCTTCCATTTTTGCTCAATCCAACCTCTCCTATCTCTCCACGCAACCAAACCCCTTCCGCTTCTTCCTCTACTCCAAGACCCACCGAAACGCCGCCGTTCCCTCTCTCAGACTCCGCTCATGGCCCACTTCGACGCCGCCGAGCCTCCGAGTAGCCACCTCGGTTCGGAGCGCCTCCAAAGAAGAAGAACCAGCGCCGCCCAAGAAGCAGCAGGGGAAAAGGACCGACCTGAAGAAGATTCTGATACTGGGCGCCGGCCCCATTGTGATCGGGCAAGCCTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAGGCTCTGAAAGAAGAAGGATACGACGTCGTTTTGATCAATTCCAACCCGGCCACCATCATGACCGACCCCGATTTGGCCGACCGGACTTACATTACGCCGATGACGCCGGAGCTGGTGGAGCAAGTCCTCGAAAAGGAGCGCCCCAACGCTCTCCTCCCCACCATGGGAGGCCAGACGGCGTTGAACCTCGCCGTTGCGTTAGCCGAGAGCGGTGCGCTTGAAAAGTATGGGGTGGAGTTGATCGGAGCCAAGCTGGAGGCCATAAAGAAAGCAGAGGATAGGGATTTGTTCAAGCAGGCCATGAACAACATTGGGATCAAAACGCCGCCGTCTGGAATTGCCAACACTTATGAGGAATGCCTCGAAATCGCGAATGAGATTGGTGAGTTTCCCTTGATCATTAGGCCGGCTTTTACTCTAGGAGGGACGGGAGGTGGAATTGCTTATAATAAGGAGGAGTTTGAGACTATCTGTAAGTCGGGGCTGGCGGCGAGCTTGACTTGTCAGATTTTGGTGGAGAAGTCATTGTTGGGGTGGAAGGAGTATGAGCTTGAGGTCATGAGGGACTTGGCAGACAATGTGGTTATTATTTGCTCCATTGAGAATATTGACCCAATGGGGGTTCATACTGGGGATTCTATCACCGTGGCGCCTGCGCAGACTTTGACTGATAAGGAGTATCAGCGGCTAAGAGACTATTCCATTGCTATAATAAGGGAGATTGGAGTGGAATGTGGTGGCTCTAATGTGCAGTTTGCGGTGAATCCCAAAGATGGGGAGGTTATGGTGATTGAGATGAATCCTAGGGTGTCGCGGTCCTCCGCTCTCGCATCCAAAGCTACTGGATTTCCGATAGCGAAAATGGCTGCCAAGTTGTCAGTTGGGTATTCATTGGATCAAATTCCCAATGACATTACAAAGAAGACACCGGCTAGTTTTGAACCTTCCATAGACTATGTGGTAACAAAGATTCCCAGGTTTGCTTTTGAGAAATTTCCTGGTTCACAGCCAATATTGACGACCCAGATGAAATCTGTTGGTGAGTCAATGGCTCTGGGTCGTACATTTCAAGAGTCCTTTCAGAAGGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGCAAAATTAAGGAACTGGACTGGGGTTGGGACAAGTTGAAATATAGCCTCCGAGTCCCTAACCCAGATCGAATCCATGCCGTATATGCTGCAATGAAGAAAGGGATGAAGGTAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTCAAGGAGTTGGTAGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGAGTTCTACGAAGTGAAGAAAAGAGGCTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCTTCTGAAAATGAAGTGCGGTTGAAGAGACTATTGTTAGGTGTTACTCCAGCATATAAGCGGGTAGATACGTGCGCTGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCTTCCTATGACTTTGAGTGTGAATCAGCTCCAACCCAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAAACAATTATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACGAGCGACCGTCTATATTTTGAGCCACTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTCGGAGGACAAACACCTCTGAAGCTGGCACTTCCCCTCCAGCAGTATTTAGACGAAAACAAGCCGAGATGTGCAAGTGGGATTGGACATGTACGCATTTGGGGTACAACACCTGACTCAATAGATGCTGCTGAGGACAGGGAGAAGTTCAATGCAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGAATTGCAAAGAGCGAAGCAGATGCTCTTGCAATTGCAACAGACATTGGCTACCCCGTTGTTGTCCGACCTTCCTATGTATTAGGTGGCCGTGCCATGGAGATAGTCTATAGTGATGAAAAACTTGTGACCTACCTTGAAACTGCTGTTGAGGTGGACCCAGAACGCCCTGTATTGATTGACAGGTATCTATCAGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATTGAGCAGGCTGGGGTCCATTCTGGTGACTCTGCTTGCTCAATTCCAACAAAAACCATCCCAGCTTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCCAAGAGGCTGAATGTGTGCGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGTGATGTTTATTTACTTGAGGCAAATCCTCGTGCTTCCCGTACAGTCCCATTTGTGTCCAAGGCAATTGGACATCCATTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGACCTTAATTTCACAAAAGAAGTCATTCCTGCACATGTGTCAGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTACTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCCATTGCATTTGCCAAGGCGCAGATTGCTGCTGGACAGAAGCCACCACTTTCTGGCACTGTGTTCTTGAGCTTAAATGACTTAACCAAACCTCATCTTGAAAGGATTGCAACCGCATTTTTAGGCCTTGGATTTAAGATTGTTTCAACTTCAGGGACAGCTCATTTTCTTGAATTAGCGAAAATTCCCGTGGAACAAGTGCTGAAGCTGCATGAGGGGCGACCTAATGCTGGTGACATGGTTGCAAATGGACAAATCCAGTTGATGGTGATCACAAGTTCTGGGGATGCACATGATCAAATTGATGGACGGCAGCTAAGGAGGATGGCTCTTGCTTACAAGATTCCAGTAATTACCACAGTTGCCGGAGCATTGGCCACTGCAGAAGCAATAAAGAGTCTAAAGTCCAGCTCCATTAAAATGATTGCTCTTCAGGACTTTTTTGATGATGAGAACAAAGCTCTCGGTAATAAGAAGTTGCAGTCAGCCTCGTCCTATATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1188

Amino Acids

130.19

Weight (kDa)

5.6

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	90 - 207	1.8e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	211 - 418	1.3e-71	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	510 - 588	4.6e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	640 - 765	8e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	769 - 972	6e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	777 - 943	8.3e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	796 - 941	7.1e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1055 - 1140	3.9e-20	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	801
AatII	GACGTC	1 cut(s)	381
Acc16I	TGCGCA	1 cut(s)	1011
AccB1I	GGYRCC	2 cut(s)	290, 1003
AccB7I	CCANNNNNTGG	2 cut(s)	3116, 3328
AccBSI	CCGCTC	3 cut(s)	156, 564, 1178
AccII	CGCG	2 cut(s)	725, 1168
AclWI	GGATC	3 cut(s)	677, 1257, 2165
AcoI	YGGCCR	5 cut(s)	402, 429, 2449, 2736, 3438
AcsI	RAATTY	6 cut(s)	1254, 1346, 1602, 1976, 2044, 3246
AcuI	CTGAAG	7 cut(s)	290, 1723, 1997, 2210, 3198, 3287, 3479
AcyI	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
AfaI	GTAC	6 cut(s)	1417, 1880, 2262, 2275, 2836, 3006
AflIII	ACRYGT	2 cut(s)	2257, 2950
AjnI	CCWGG	4 cut(s)	1328, 1351, 1677, 2889
AjuI	GAANNNNNNNTTGG	2 cut(s)	866, 898
Alw21I	GWGCWC	1 cut(s)	2631
Alw26I	GTCTC	5 cut(s)	23, 812, 1041, 1792, 2913
AlwI	GGATC	3 cut(s)	677, 1257, 2165
Ama87I	CYCGRG	1 cut(s)	334
ApeKI	GCWGC	9 cut(s)	12, 247, 1220, 1571, 1847, 2297, 3092, 3271, 3376
ApoI	RAATTY	6 cut(s)	1254, 1346, 1602, 1976, 2044, 3246
Asp700I	GAANNNNTTC	2 cut(s)	2940, 2970
AspA2I	CCTAGG	1 cut(s)	1157
AspLEI	GCGC	9 cut(s)	212, 235, 293, 495, 571, 1006, 1012, 1847, 3085
AspS9I	GGNCC	9 cut(s)	4, 162, 261, 296, 1170, 1949, 2518, 2647, 3014
AsuC2I	CCSGG	1 cut(s)	401
AvaI	CYCGRG	1 cut(s)	334
AvaII	GGWCC	5 cut(s)	261, 1170, 1949, 2518, 2647
AvrII	CCTAGG	1 cut(s)	1157
BalI	TGGCCA	2 cut(s)	2738, 3440
BanI	GGYRCC	2 cut(s)	290, 1003
BauI	CACGAG	2 cut(s)	2080, 2823
BbsI	GAAGAC	1 cut(s)	1283
Bbv12I	GWGCWC	1 cut(s)	2631
BbvCI	CCTCAGC	1 cut(s)	2301
BbvI	GCAGC	8 cut(s)	259, 1207, 1558, 1834, 2284, 3079, 3258, 3388
BccI	CCATC	9 cut(s)	416, 1121, 1467, 2147, 2696, 3037, 3322, 3362, 3382
BceAI	ACGGC	7 cut(s)	119, 530, 546, 667, 1547, 2436, 3389
BcgI	CGANNNNNNTGC	4 cut(s)	548, 582, 2366, 2400
BciT130I	CCWGG	4 cut(s)	1330, 1353, 1679, 2891
BciVI	GTATCC	1 cut(s)	365
BclI	TGATCA	4 cut(s)	387, 750, 3333, 3358
BcnI	CCSGG	1 cut(s)	401
BcoDI	GTCTC	5 cut(s)	23, 812, 1041, 1792, 2913
BfaI	CTAG	4 cut(s)	773, 1158, 1287, 1683
BfmI	CTRYAG	3 cut(s)	1848, 2470, 3444
BfoI	RGCGCY	5 cut(s)	213, 236, 294, 496, 1007
BfuI	GTATCC	1 cut(s)	365
BlnI	CCTAGG	1 cut(s)	1157
Bme1390I	CCNGG	5 cut(s)	401, 1330, 1353, 1679, 2891
Bme18I	GGWCC	5 cut(s)	261, 1170, 1949, 2518, 2647
BmeT110I	CYCGRG	1 cut(s)	334
BmgT120I	GGNCC	9 cut(s)	4, 162, 261, 296, 1170, 1949, 2518, 2647, 3014
BmiI	GGNNCC	7 cut(s)	292, 298, 601, 1005, 1634, 2520, 2648
BmrFI	CCNGG	5 cut(s)	401, 1330, 1353, 1679, 2891
BmrI	ACTGGG	3 cut(s)	296, 993, 1513
BmsI	GCATC	7 cut(s)	1193, 2284, 2374, 2548, 2569, 3012, 3340
BmuI	ACTGGG	3 cut(s)	296, 993, 1513
BpiI	GAAGAC	1 cut(s)	1283
BplI	GAGNNNNNCTC	2 cut(s)	3376, 3408
BpmI	CTGGAG	3 cut(s)	629, 1803, 2192
Bpu10I	CCTNAGC	2 cut(s)	2301, 3380
BpuEI	CTTGAG	5 cut(s)	327, 926, 1631, 2831, 3151
BpuMI	CCSGG	1 cut(s)	401
BsaAI	YACGTR	1 cut(s)	1842
BsaBI	GATNNNNATC	1 cut(s)	3332
BsaHI	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
BsaWI	WCCGGW	1 cut(s)	435
BsaXI	ACNNNNNCTCC	4 cut(s)	16, 46, 2190, 2220
Bse118I	RCCGGY	3 cut(s)	293, 760, 1282
Bse1I	ACTGG	8 cut(s)	291, 988, 1201, 1503, 1508, 3037, 3322, 3410
Bse3DI	GCAATG	4 cut(s)	7, 1056, 1581, 3066
Bse8I	GATNNNNATC	1 cut(s)	3332
BseBI	CCWGG	4 cut(s)	1330, 1353, 1679, 2891
BseGI	GGATG	9 cut(s)	1184, 1478, 1593, 1714, 2000, 2688, 2866, 3355, 3393
BseJI	GATNNNNATC	1 cut(s)	3332
BseMI	GCAATG	4 cut(s)	7, 1056, 1581, 3066
BseMII	CTCAG	4 cut(s)	160, 1604, 2292, 3009
BseNI	ACTGG	8 cut(s)	291, 988, 1201, 1503, 1508, 3037, 3322, 3410
BseRI	GAGGAG	3 cut(s)	24, 497, 824
BseXI	GCAGC	8 cut(s)	259, 1207, 1558, 1834, 2284, 3079, 3258, 3388
BseYI	CCCAGC	2 cut(s)	2642, 2692
BsgI	GTGCAG	3 cut(s)	1124, 2186, 2931
Bsh1236I	CGCG	2 cut(s)	725, 1168
Bsh1285I	CGRYCG	2 cut(s)	436, 2089
BshNI	GGYRCC	2 cut(s)	290, 1003
BsiEI	CGRYCG	2 cut(s)	436, 2089
BsiHKAI	GWGCWC	1 cut(s)	2631
BsiHKCI	CYCGRG	1 cut(s)	334
BsiSI	CCGG	7 cut(s)	294, 401, 436, 461, 761, 1283, 3429
BslFI	GGGAC	7 cut(s)	352, 793, 932, 1523, 1526, 2825, 3232
BsmAI	GTCTC	5 cut(s)	23, 812, 1041, 1792, 2913
BsmFI	GGGAC	7 cut(s)	352, 793, 932, 1523, 1526, 2825, 3232
BsmI	GAATGC	1 cut(s)	716
BsoBI	CYCGRG	1 cut(s)	334
Bsp1286I	GDGCHC	1 cut(s)	2631
Bsp19I	CCATGG	2 cut(s)	516, 2457
Bsp68I	TCGCGA	1 cut(s)	725
BspCNI	CTCAG	4 cut(s)	159, 1605, 2293, 3010
BspFNI	CGCG	2 cut(s)	725, 1168
BspHI	TCATGA	4 cut(s)	411, 912, 1606, 2914
BspLI	GGNNCC	7 cut(s)	292, 298, 601, 1005, 1634, 2520, 2648
BspMAI	CTGCAG	2 cut(s)	1852, 3448
BspPI	GGATC	3 cut(s)	677, 1257, 2165
BspQI	GCTCTTC	1 cut(s)	3498
BspT107I	GGYRCC	2 cut(s)	290, 1003
BsrBI	CCGCTC	3 cut(s)	156, 564, 1178
BsrDI	GCAATG	4 cut(s)	7, 1056, 1581, 3066
BsrFI	RCCGGY	3 cut(s)	293, 760, 1282
BsrI	ACTGG	8 cut(s)	291, 988, 1201, 1503, 1508, 3037, 3322, 3410
BssAI	RCCGGY	3 cut(s)	293, 760, 1282
BssNI	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
BssSI	CACGAG	2 cut(s)	2080, 2823
BssT1I	CCWWGG	7 cut(s)	516, 1157, 2457, 2853, 2992, 3078, 3190
Bst2BI	CACGAG	2 cut(s)	2080, 2823
Bst2UI	CCWGG	4 cut(s)	1330, 1353, 1679, 2891
Bst4CI	ACNGT	8 cut(s)	1000, 2062, 2090, 2113, 2771, 2839, 3124, 3424
Bst6I	CTCTTC	5 cut(s)	46, 1790, 1888, 2916, 3498
BstACI	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
BstBAI	YACGTR	1 cut(s)	1842
BstDEI	CTNAG	6 cut(s)	146, 1043, 1613, 2301, 3018, 3380
BstDSI	CCRYGG	4 cut(s)	516, 999, 2457, 3252
BstENI	CCTNNNNNAGG	1 cut(s)	485
BstF5I	GGATG	9 cut(s)	1184, 1478, 1593, 1714, 2000, 2688, 2866, 3355, 3393
BstFNI	CGCG	2 cut(s)	725, 1168
BstH2I	RGCGCY	5 cut(s)	213, 236, 294, 496, 1007
BstHHI	GCGC	9 cut(s)	212, 235, 293, 495, 571, 1006, 1012, 1847, 3085
BstMAI	GTCTC	5 cut(s)	23, 812, 1041, 1792, 2913
BstMCI	CGRYCG	2 cut(s)	436, 2089
BstNI	CCWGG	4 cut(s)	1330, 1353, 1679, 2891
BstNSI	RCATGY	2 cut(s)	2261, 2954
BstSCI	CCNGG	5 cut(s)	399, 1328, 1351, 1677, 2889
BstSFI	CTRYAG	3 cut(s)	1848, 2470, 3444
BstUI	CGCG	2 cut(s)	725, 1168
BstV1I	GCAGC	8 cut(s)	259, 1207, 1558, 1834, 2284, 3079, 3258, 3388
BstV2I	GAAGAC	1 cut(s)	1283
BsuI	GTATCC	1 cut(s)	365
BtgI	CCRYGG	4 cut(s)	516, 999, 2457, 3252
BtsCI	GGATG	9 cut(s)	1184, 1478, 1593, 1714, 2000, 2688, 2866, 3355, 3393
BtsI	GCAGTG	1 cut(s)	3441
BtsIMutI	CAGTG	5 cut(s)	1747, 2105, 3030, 3120, 3441
BtuMI	TCGCGA	1 cut(s)	725
CciI	TCATGA	4 cut(s)	411, 912, 1606, 2914
CfoI	GCGC	9 cut(s)	212, 235, 293, 495, 571, 1006, 1012, 1847, 3085
Cfr10I	RCCGGY	3 cut(s)	293, 760, 1282
Cfr13I	GGNCC	9 cut(s)	4, 162, 261, 296, 1170, 1949, 2518, 2647, 3014
CseI	GACGC	2 cut(s)	181, 466
Csp6I	GTAC	6 cut(s)	1416, 1879, 2261, 2274, 2835, 3005
CviQI	GTAC	6 cut(s)	1416, 1879, 2261, 2274, 2835, 3005
DdeI	CTNAG	6 cut(s)	146, 1043, 1613, 2301, 3018, 3380
DinI	GGCGCC	2 cut(s)	292, 1005
EaeI	YGGCCR	5 cut(s)	402, 429, 2449, 2736, 3438
Eam1104I	CTCTTC	5 cut(s)	46, 1790, 1888, 2916, 3498
EarI	CTCTTC	5 cut(s)	46, 1790, 1888, 2916, 3498
Eco130I	CCWWGG	7 cut(s)	516, 1157, 2457, 2853, 2992, 3078, 3190
Eco147I	AGGCCT	1 cut(s)	3189
Eco47I	GGWCC	5 cut(s)	261, 1170, 1949, 2518, 2647
Eco57I	CTGAAG	7 cut(s)	290, 1723, 1997, 2210, 3198, 3287, 3479
Eco88I	CYCGRG	1 cut(s)	334
EcoNI	CCTNNNNNAGG	1 cut(s)	485
EcoRI	GAATTC	1 cut(s)	2044
EcoRII	CCWGG	4 cut(s)	1328, 1351, 1677, 2889
EcoT14I	CCWWGG	7 cut(s)	516, 1157, 2457, 2853, 2992, 3078, 3190
EgeI	GGCGCC	2 cut(s)	292, 1005
EheI	GGCGCC	2 cut(s)	292, 1005
ErhI	CCWWGG	7 cut(s)	516, 1157, 2457, 2853, 2992, 3078, 3190
FalI	AAGNNNNNCTT	2 cut(s)	2958, 2990
FaqI	GGGAC	7 cut(s)	352, 793, 932, 1523, 1526, 2825, 3232
FauI	CCCGC	2 cut(s)	1824, 2750
FbaI	TGATCA	4 cut(s)	387, 750, 3333, 3358
FokI	GGATG	9 cut(s)	1171, 1485, 1600, 1721, 1987, 2675, 2853, 3362, 3400
FspBI	CTAG	4 cut(s)	773, 1158, 1287, 1683
FspI	TGCGCA	1 cut(s)	1011
GlaI	GCGC	9 cut(s)	211, 234, 292, 494, 570, 1005, 1011, 1846, 3084
GsaI	CCCAGC	2 cut(s)	2646, 2696
GsuI	CTGGAG	3 cut(s)	629, 1803, 2192
HaeII	RGCGCY	5 cut(s)	213, 236, 294, 496, 1007
HapII	CCGG	7 cut(s)	294, 401, 436, 461, 761, 1283, 3429
HgaI	GACGC	2 cut(s)	181, 466
HhaI	GCGC	9 cut(s)	212, 235, 293, 495, 571, 1006, 1012, 1847, 3085
Hin1I	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
Hin6I	GCGC	9 cut(s)	210, 233, 291, 493, 569, 1004, 1010, 1845, 3083
HinP1I	GCGC	9 cut(s)	210, 233, 291, 493, 569, 1004, 1010, 1845, 3083
HindIII	AAGCTT	1 cut(s)	345
HpaII	CCGG	7 cut(s)	294, 401, 436, 461, 761, 1283, 3429
Hpy166II	GTNNAC	2 cut(s)	1358, 2518
Hpy8I	GTNNAC	2 cut(s)	1358, 2518
Hpy99I	CGWCG	3 cut(s)	175, 380, 383
HpyAV	CCTTC	9 cut(s)	103, 362, 886, 1308, 1432, 1585, 1921, 2021, 2442
HpyCH4III	ACNGT	8 cut(s)	1000, 2062, 2090, 2113, 2771, 2839, 3124, 3424
HpyCH4IV	ACGT	2 cut(s)	378, 1841
HpyF3I	CTNAG	6 cut(s)	146, 1043, 1613, 2301, 3018, 3380
HpySE526I	ACGT	2 cut(s)	378, 1841
Hsp92I	GRCGYC	5 cut(s)	173, 291, 378, 458, 1004
HspAI	GCGC	9 cut(s)	210, 233, 291, 493, 569, 1004, 1010, 1845, 3083
KasI	GGCGCC	2 cut(s)	290, 1003
KroI	GCCGGC	2 cut(s)	293, 760
KroNI	GCCGGC	2 cut(s)	295, 762
Ksp22I	TGATCA	4 cut(s)	387, 750, 3333, 3358
LguI	GCTCTTC	1 cut(s)	3498
Lsp1109I	GCAGC	8 cut(s)	259, 1207, 1558, 1834, 2284, 3079, 3258, 3388
LweI	GCATC	7 cut(s)	1193, 2284, 2374, 2548, 2569, 3012, 3340
MaeI	CTAG	4 cut(s)	773, 1158, 1287, 1683
MaeII	ACGT	2 cut(s)	378, 1841
MaeIII	GTNAC	6 cut(s)	1315, 1742, 1813, 2488, 2657, 3296
MbiI	CCGCTC	3 cut(s)	156, 564, 1178
MfeI	CAATTG	2 cut(s)	2394, 2859
MhlI	GDGCHC	1 cut(s)	2631
MlsI	TGGCCA	2 cut(s)	2738, 3440
MluNI	TGGCCA	2 cut(s)	2738, 3440
Mly113I	GGCGCC	2 cut(s)	291, 1004
MmeI	TCCRAC	5 cut(s)	84, 420, 1937, 2452, 2702
Mox20I	TGGCCA	2 cut(s)	2738, 3440
MroNI	GCCGGC	2 cut(s)	293, 760
MroXI	GAANNNNTTC	2 cut(s)	2940, 2970
MscI	TGGCCA	2 cut(s)	2738, 3440
MseI	TTAA	8 cut(s)	1491, 2331, 2339, 2922, 3137, 3146, 3198, 3480
MslI	CAYNNNNRTG	4 cut(s)	410, 2601, 2655, 3482
Msp20I	TGGCCA	2 cut(s)	2738, 3440
MspA1I	CMGCKG	1 cut(s)	1039
MspI	CCGG	7 cut(s)	294, 401, 436, 461, 761, 1283, 3429
MspR9I	CCNGG	5 cut(s)	401, 1330, 1353, 1679, 2891
MunI	CAATTG	2 cut(s)	2394, 2859
Mva1269I	GAATGC	1 cut(s)	716
MvaI	CCWGG	4 cut(s)	1330, 1353, 1679, 2891
MvnI	CGCG	2 cut(s)	725, 1168
NaeI	GCCGGC	2 cut(s)	295, 762
NarI	GGCGCC	2 cut(s)	291, 1004
NciI	CCSGG	1 cut(s)	401
NcoI	CCATGG	2 cut(s)	516, 2457
NgoMIV	GCCGGC	2 cut(s)	293, 760
NlaIV	GGNNCC	7 cut(s)	292, 298, 601, 1005, 1634, 2520, 2648
NmeAIII	GCCGAG	3 cut(s)	204, 583, 2259
NmuCI	GTSAC	4 cut(s)	1742, 2488, 2657, 3296
NruI	TCGCGA	1 cut(s)	725
NsbI	TGCGCA	1 cut(s)	1011
NspI	RCATGY	2 cut(s)	2261, 2954
PagI	TCATGA	4 cut(s)	411, 912, 1606, 2914
PceI	AGGCCT	1 cut(s)	3189
PciI	ACATGT	2 cut(s)	2257, 2950
PciSI	GCTCTTC	1 cut(s)	3498
PcsI	WCGNNNNNNNCGW	2 cut(s)	176, 2830
PctI	GAATGC	1 cut(s)	716
PdiI	GCCGGC	2 cut(s)	295, 762
PdmI	GAANNNNTTC	2 cut(s)	2940, 2970
PfeI	GAWTC	9 cut(s)	19, 277, 989, 1117, 1153, 1324, 1554, 1904, 3406
PflMI	CCANNNNNTGG	2 cut(s)	3116, 3328
PluTI	GGCGCC	2 cut(s)	294, 1007
Ppu21I	YACGTR	1 cut(s)	1842
PscI	ACATGT	2 cut(s)	2257, 2950
PsiI	TTATAA	1 cut(s)	801
Psp6I	CCWGG	4 cut(s)	1328, 1351, 1677, 2889
PspFI	CCCAGC	2 cut(s)	2642, 2692
PspGI	CCWGG	4 cut(s)	1328, 1351, 1677, 2889
PspN4I	GGNNCC	7 cut(s)	292, 298, 601, 1005, 1634, 2520, 2648
PspPI	GGNCC	9 cut(s)	4, 162, 261, 296, 1170, 1949, 2518, 2647, 3014
PstI	CTGCAG	2 cut(s)	1852, 3448
RruI	TCGCGA	1 cut(s)	725
RsaI	GTAC	6 cut(s)	1417, 1880, 2262, 2275, 2836, 3006
RsaNI	GTAC	6 cut(s)	1416, 1879, 2261, 2274, 2835, 3005
RseI	CAYNNNNRTG	4 cut(s)	410, 2601, 2655, 3482
SapI	GCTCTTC	1 cut(s)	3498
SaqAI	TTAA	8 cut(s)	1491, 2331, 2339, 2922, 3137, 3146, 3198, 3480
Sau96I	GGNCC	9 cut(s)	4, 162, 261, 296, 1170, 1949, 2518, 2647, 3014
ScrFI	CCNGG	5 cut(s)	401, 1330, 1353, 1679, 2891
SduI	GDGCHC	1 cut(s)	2631
SfaNI	GCATC	7 cut(s)	1193, 2284, 2374, 2548, 2569, 3012, 3340
SfcI	CTRYAG	3 cut(s)	1848, 2470, 3444
SfoI	GGCGCC	2 cut(s)	292, 1005
SinI	GGWCC	5 cut(s)	261, 1170, 1949, 2518, 2647
SmiMI	CAYNNNNRTG	4 cut(s)	410, 2601, 2655, 3482
SmlI	CTYRAG	5 cut(s)	342, 905, 1646, 2810, 3130
SmoI	CTYRAG	5 cut(s)	342, 905, 1646, 2810, 3130
SseBI	AGGCCT	1 cut(s)	3189
SspDI	GGCGCC	2 cut(s)	290, 1003
SspI	AATATT	2 cut(s)	961, 1368
SspMI	CTAG	4 cut(s)	773, 1158, 1287, 1683
StuI	AGGCCT	1 cut(s)	3189
StyD4I	CCNGG	5 cut(s)	399, 1328, 1351, 1677, 2889
StyI	CCWWGG	7 cut(s)	516, 1157, 2457, 2853, 2992, 3078, 3190
TaaI	ACNGT	8 cut(s)	1000, 2062, 2090, 2113, 2771, 2839, 3124, 3424
TaiI	ACGT	2 cut(s)	381, 1844
TaqI	TCGA	7 cut(s)	170, 327, 483, 717, 1447, 1552, 1957
TaqII	GACCGA	2 cut(s)	278, 431
TatI	WGTACW	2 cut(s)	1878, 3004
TauI	GCSGC	6 cut(s)	134, 178, 238, 682, 845, 1042
TfiI	GAWTC	9 cut(s)	19, 277, 989, 1117, 1153, 1324, 1554, 1904, 3406
Tru1I	TTAA	8 cut(s)	1491, 2331, 2339, 2922, 3137, 3146, 3198, 3480
Tru9I	TTAA	8 cut(s)	1491, 2331, 2339, 2922, 3137, 3146, 3198, 3480
TscAI	CASTG	5 cut(s)	1747, 2112, 3037, 3127, 3448
TseFI	GTSAC	4 cut(s)	1742, 2488, 2657, 3296
TseI	GCWGC	9 cut(s)	12, 247, 1220, 1571, 1847, 2297, 3092, 3271, 3376
Tsp45I	GTSAC	4 cut(s)	1742, 2488, 2657, 3296
TspRI	CASTG	5 cut(s)	1747, 2112, 3037, 3127, 3448
Van91I	CCANNNNNTGG	2 cut(s)	3116, 3328
VpaK11BI	GGWCC	5 cut(s)	261, 1170, 1949, 2518, 2647
XagI	CCTNNNNNAGG	1 cut(s)	485
XapI	RAATTY	6 cut(s)	1254, 1346, 1602, 1976, 2044, 3246
XceI	RCATGY	2 cut(s)	2261, 2954
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1309
XmaJI	CCTAGG	1 cut(s)	1157
XmnI	GAANNNNTTC	2 cut(s)	2940, 2970
XspI	CTAG	4 cut(s)	773, 1158, 1287, 1683
ZraI	GACGTC	1 cut(s)	379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.