Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

11513526 .. 11518712

Gene Structure 5187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1125100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3597 bp

ATGGACCGTTGCCTCAGCCACGGCGAGTCTCTCTCCCCGAAGCTTTCAATCTTCGGCAACTCCAAGCCTTATTCGTCGAAACCAAACAGCTTCCGCTTCGTCCTCTACTCCAAGAAGTTCGATGCGCGAAACGCTGCCGTTCCTTCTCTGCGCCTCCGATCATTTCCGTCGCAGCTCGCCACTTTCACTAACCGTCCGAGTTGTCGCCGAGTCAACTCGGTCCGGTGCGATTCGGTTAATGACGGGGCTGCTGCGGCGAAAGATGGGGCGCCGCCGAAGCTGGGGAAGAGGACGGACATTAAGAAGATTATGATTCTCGGAGCCGGGCCGATTGTGATAGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAGGCGTGCAAGGCGCTGAAGGAGGATGGATACGAGGTCGTTTTGATCAATTCCAACCCGGCCACGATCATGACTGACCCGGACTTGGCCGACAGGACTTACATTACGCCGATGACGCCCGAGCTGGTCGAGCAGGTTCTCGAGAAGGAGCGGCCCGACGCTCTATTGCCAACTATGGGCGGCCAGACGGCGCTGAATTTGGCTGTGGCGCTGGCCGAGAGCGGGGCTCTGGCTAAATACGGAGTGGAATTGATCGGGGCAAAGCTGGAGGCCATCAAAAAAGCAGAGGACCGGGAATTGTTCAAGCAGGCCATGAAGAACATTGGTGTGAAAACCCCGCCATCAGGGATTGCCACGACGCTGGAGGAATGTATCGAAATTGCAAATGAAATTGGAGAGTTTCCACTGATTATTAGGCCAGCGTTTACTTTGGGAGGGACTGGAGGTGGCATTGCTTATAACAGAGAAGAGTTTGAGGACATTTGTAAGGCAGGGATTGCTGCGAGTGTGACGTCTCAGGTTTTGGTGGAGAAGTCGTTGTTGGGATGGAAGGAGTATGAGCTTGAGGTGATGAGGGACTTGGCTGACAATGTGGTGATTATATGCTCCATTGAGAACATCGACCCAATGGGGGTTCATACCGGGGACTCTATCACTGTGGCACCGGCTCAAACTTTGACGGATAAGGAGTATCAGCGGCTGAGGGACTATTCCATTGCTATCATAAGGGAGATTGGAGTGGAATGTGGCGGTTCCAATGTGCAGTTTGCCGTTAATCCCGTTGATGGTGAGGTTATGGTGATTGAAATGAACCCGAGGGTATCTCGTTCCTCGGCTTTGGCATCCAAAGCTACCGGCTTTCCCATTGCGAAAATGGCTGCCAAGTTGTCGGTAGGCTATTCATTGGATCAGATACCTAATGACATTACAAAGAAGACCCCGGCTAGCTTTGAGCCTTCCATAGATTATGTAGTAACCAAGATTCCCAGGTTTGCATTTGAAAAATTTCCAGGTTCACAACCAATTTTGACTACACAAATGAAATCTGTTGGTGAGTCAATGGCGCTGGGTCGCACGTTTCAAGAGTCTTTTCAGAAAGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGCTAAAATTAAGGAGCTTGACTGGGATTGGGAACAATTGAAGTATAGCCTCCGAGTCCCTAACCCAGAGCGTATCCATGCCATATATGCTGCAATGAAGAGGGGTATGAAGGTCGATGATATTCATGAGTTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAGGAGCTGGTAGATGTGGAGCAATACCTTCTGGCAAGAAACTTGTCTGATTTAACAAAGGATGAGTTCTACGAAGTGAAAAAGAGAGGTTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCCTCTGAAAAAGACGTCAGGATGAAGAGACTATCACTAGGTGTTGCTCCATCATATAAGCGGGTGGATACTTGTGCTGCAGAGTTTGAGGCCAATACTCCTTACATGTACTCTTCCTATGACTATGAGTGTGAATCAGCTCCCACCCAAAGTAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATCGGTCAAGGGATCGAGTTTGACTACTGTTGTTGCCATACGTCCTTTGCACTTCAGAAAGCAGGATATGAGACAATTATGATGAATTCAAATCCTGAAACGGTATCCACTGATTATGACACAAGTGACCGTCTATATTTTGAGCCACTGACTGTCGAAGATGTGCTGAATATAATTGATTTGGAAAGACCTGATGGTATCATTGTGCAGTTTGGAGGTCAAACACCACTGAAGCTGTCTCTTCCTATTCAGCGATATCTAGATGAGAACAAGCCTGAATGTGCTAGTGGGAACGGACATGTACGCATTTGGGGTACTTCACCAGCTTCCATAGATGATGCTGAGGACAGGGAAAAGTTCAATACAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGTATTGCCAAGAGCGAAGCTGATGCTGTTGCCATCGCTAAGGACATCGGGTACCCAGTTGTTGTCCGGCCTTCTTATGTATTGGGTGGCCGAGCCATGGAGATTGTATATAGCGACGAAAAACTTGCGACCTATCTCGAAAATGCTGTTGAAGTGGACCCAGAGCGCCCTGTGTTGATTGACAAGTATCTATCTGATGCCATTGAGATTGATGTCGATGCACTTGCCGACTCTCAGGGGAATGTTGTCATTGGTGGGATAATGGAGCACATTGAACAGGCTGGAGTCCATTCTGGTGACTCTGCTTGCTCGATTCCAACAAAAACCATCCCTGCTTCTTGCTTAGACACAATTAGGAAATGGACTACAAAATTGGCTAAGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACCTTGTCTGGAGACGTTTTCTTACTTGAGGCAAACCCTCGTGCTTCCCGTACTGTCCCTTTTGTGTCCAAGGCGATTGGGCATCCGTTGGCTAAATATGCTTCTCTTGTCATGTCTGGGAAGTCTCTTCATGATCTAAATTTCACAAAAGAGGTCATTCCTGCACATGTATCAGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCCGGGATGTGATGTGTTGCTGGGGCCTGAGATGCGTAGCACTGGGGAGGTGATGGGTATTGATTACGAGTTTCCCATTGCATTCGCCAAGGCTCAGATTTCTGCTGGGCAGAAGCTGCCACTTACTGGCACTGTGTTCCTCAGCTTAAATGATTTGACCAAACCTCATCTTGAAAAGATTGCCAAAGCCTTTTTGGGACTTGGATTTAAGATTGTTTCAACTTCTGGGACAGCTCATATTCTTGAGCTAGCAAAACTTCCAGTGGAACGAGTGCTGAAACTTCATGAAGGACGGCCTCATGCCGCTGATATGGTTGCTAATGGCCAAATCCAGTTAATGGTGATCACAAGTTCGGGTGATGCACTTGATCAGATTGATGGACGGCAACTGAGGAGGTCGGGCCTTGCTTACAAGATTCCAGTAATAACAACTATTGCCGGAGCTTTGTCAACCGCAGAAGCAATAAAGAGCTTAAAGTCTAGCACTATTAAGATGATTGCACTGCAGGACTTCTTTGAGGATGAGAACAAAGCTGGGAGTGATAAAACATTGCAGTCAGTCACTTCCTACCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1199

Amino Acids

131.03

Weight (kDa)

5.56

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	100 - 217	2e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	221 - 428	2.7e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	520 - 598	2.7e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	651 - 775	8e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	779 - 982	5.5e-37	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	787 - 953	4e-07	ATP-grasp domain
Dala_Dala_lig_C	PF07478	806 - 951	5e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1064 - 1150	2.6e-18	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	831
AatII	GACGTC	2 cut(s)	887, 1818
Acc36I	ACCTGC	1 cut(s)	496
Acc65I	GGTACC	1 cut(s)	2442
AccB1I	GGYRCC	3 cut(s)	268, 1033, 2442
AccB7I	CCANNNNNTGG	2 cut(s)	3146, 3358
AccBSI	CCGCTC	2 cut(s)	523, 594
AccII	CGCG	1 cut(s)	127
AclWI	GGATC	2 cut(s)	1287, 2009
AcoI	YGGCCR	6 cut(s)	432, 459, 553, 585, 2479, 3343
AcsI	RAATTY	4 cut(s)	568, 1376, 2074, 2953
AcuI	CTGAAG	3 cut(s)	410, 2027, 2240
AcyI	GRCGYC	4 cut(s)	269, 488, 884, 1815
AdeI	CACNNNGTG	1 cut(s)	1841
AfaI	GTAC	5 cut(s)	1910, 2292, 2305, 2444, 2866
AflII	CTTAAG	1 cut(s)	1676
AflIII	ACRYGT	3 cut(s)	1905, 2287, 2980
AjnI	CCWGG	2 cut(s)	1358, 1381
AjuI	GAANNNNNNNTTGG	2 cut(s)	896, 928
Alw21I	GWGCWC	1 cut(s)	2661
Alw26I	GTCTC	7 cut(s)	33, 891, 1822, 2054, 2232, 2820, 2943
AlwI	GGATC	2 cut(s)	1287, 2009
AlwNI	CAGNNNCTG	2 cut(s)	1072, 3136
Ama87I	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
ApeKI	GCWGC	9 cut(s)	134, 172, 248, 251, 872, 1250, 1601, 1877, 3136
ApoI	RAATTY	4 cut(s)	568, 1376, 2074, 2953
Asp700I	GAANNNNTTC	2 cut(s)	1377, 3000
Asp718I	GGTACC	1 cut(s)	2442
AspLEI	GCGC	8 cut(s)	127, 153, 271, 388, 565, 583, 1438, 2559
AspS9I	GGNCC	9 cut(s)	4, 220, 326, 525, 661, 1979, 2548, 3044, 3421
AsuC2I	CCSGG	8 cut(s)	325, 431, 452, 665, 1015, 1313, 3023, 3024
AsuNHI	GCTAGC	2 cut(s)	1316, 3268
AvaI	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
AvaII	GGWCC	5 cut(s)	4, 220, 661, 1979, 2548
BalI	TGGCCA	1 cut(s)	3345
BanI	GGYRCC	3 cut(s)	268, 1033, 2442
BanII	GRGCYC	1 cut(s)	601
BauI	CACGAG	1 cut(s)	2853
BbsI	GAAGAC	1 cut(s)	1313
Bbv12I	GWGCWC	1 cut(s)	2661
BbvCI	CCTCAGC	4 cut(s)	14, 1073, 2331, 3161
BbvI	GCAGC	9 cut(s)	121, 184, 235, 238, 859, 1237, 1588, 1864, 3123
BceAI	ACGGC	6 cut(s)	37, 122, 576, 1127, 3329, 3419
BcgI	CGANNNNNNTGC	6 cut(s)	2396, 2430, 2498, 2532, 2597, 2631
BciT130I	CCWGG	2 cut(s)	1360, 1383
BciVI	GTATCC	4 cut(s)	395, 1595, 1861, 2104
BclI	TGATCA	3 cut(s)	417, 3363, 3388
BcnI	CCSGG	8 cut(s)	325, 431, 452, 665, 1015, 1313, 3023, 3024
BcoDI	GTCTC	7 cut(s)	33, 891, 1822, 2054, 2232, 2820, 2943
BfaI	CTAG	6 cut(s)	1317, 1838, 2249, 2274, 3269, 3501
BfmI	CTRYAG	2 cut(s)	1878, 3524
BfoI	RGCGCY	6 cut(s)	272, 389, 566, 584, 1439, 2560
BfrI	CTTAAG	1 cut(s)	1676
BfuAI	ACCTGC	1 cut(s)	496
BfuI	GTATCC	4 cut(s)	395, 1595, 1861, 2104
Bme18I	GGWCC	5 cut(s)	4, 220, 661, 1979, 2548
BmeT110I	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
BmgT120I	GGNCC	9 cut(s)	4, 220, 326, 525, 661, 1979, 2548, 3044, 3421
BmiI	GGNNCC	8 cut(s)	270, 322, 1035, 1126, 1664, 2444, 2550, 3045
BmrI	ACTGGG	3 cut(s)	1543, 2441, 3072
BmsI	GCATC	9 cut(s)	112, 1223, 2317, 2404, 2578, 2599, 2905, 3042, 3370
BmtI	GCTAGC	2 cut(s)	1320, 3272
BmuI	ACTGGG	3 cut(s)	1543, 2441, 3072
BpiI	GAAGAC	1 cut(s)	1313
BplI	GAGNNNNNCTC	6 cut(s)	17, 49, 583, 615, 1180, 1212
BpmI	CTGGAG	5 cut(s)	659, 755, 834, 2694, 2844
Bpu10I	CCTNAGC	5 cut(s)	14, 1073, 2331, 2430, 3161
BpuEI	CTTGAG	3 cut(s)	956, 2861, 3284
BpuMI	CCSGG	8 cut(s)	325, 431, 452, 665, 1015, 1313, 3023, 3024
BsaHI	GRCGYC	4 cut(s)	269, 488, 884, 1815
BsaWI	WCCGGW	1 cut(s)	222
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bse118I	RCCGGY	2 cut(s)	1036, 1226
Bse1I	ACTGG	8 cut(s)	817, 1538, 2447, 3067, 3151, 3281, 3352, 3440
Bse3DI	GCAATG	6 cut(s)	822, 1086, 1236, 1611, 3096, 3569
BseBI	CCWGG	2 cut(s)	1360, 1383
BseMI	GCAATG	6 cut(s)	822, 1086, 1236, 1611, 3096, 3569
BseNI	ACTGG	8 cut(s)	817, 1538, 2447, 3067, 3151, 3281, 3352, 3440
BseRI	GAGGAG	1 cut(s)	3427
BseXI	GCAGC	9 cut(s)	121, 184, 235, 238, 859, 1237, 1588, 1864, 3123
BseYI	CCCAGC	5 cut(s)	280, 1438, 3040, 3125, 3554
BsgI	GTGCAG	3 cut(s)	1154, 2216, 2961
Bsh1236I	CGCG	1 cut(s)	127
BshNI	GGYRCC	3 cut(s)	268, 1033, 2442
BsiHKAI	GWGCWC	1 cut(s)	2661
BsiHKCI	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
BslFI	GGGAC	9 cut(s)	382, 823, 962, 1031, 1091, 1553, 2855, 3231, 3262
BsmAI	GTCTC	7 cut(s)	33, 891, 1822, 2054, 2232, 2820, 2943
BsmBI	CGTCTC	2 cut(s)	891, 2820
BsmFI	GGGAC	9 cut(s)	382, 823, 962, 1031, 1091, 1553, 2855, 3231, 3262
BsmI	GAATGC	1 cut(s)	3101
BsoBI	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
Bsp1286I	GDGCHC	2 cut(s)	601, 2661
Bsp19I	CCATGG	1 cut(s)	2487
BspFNI	CGCG	1 cut(s)	127
BspHI	TCATGA	4 cut(s)	441, 1636, 2944, 3304
BspLI	GGNNCC	8 cut(s)	270, 322, 1035, 1126, 1664, 2444, 2550, 3045
BspMAI	CTGCAG	2 cut(s)	1882, 3528
BspMI	ACCTGC	1 cut(s)	496
BspOI	GCTAGC	2 cut(s)	1320, 3272
BspPI	GGATC	2 cut(s)	1287, 2009
BspT107I	GGYRCC	3 cut(s)	268, 1033, 2442
BspTI	CTTAAG	1 cut(s)	1676
BsrBI	CCGCTC	2 cut(s)	523, 594
BsrDI	GCAATG	6 cut(s)	822, 1086, 1236, 1611, 3096, 3569
BsrFI	RCCGGY	2 cut(s)	1036, 1226
BsrI	ACTGG	8 cut(s)	817, 1538, 2447, 3067, 3151, 3281, 3352, 3440
BssAI	RCCGGY	2 cut(s)	1036, 1226
BssNI	GRCGYC	4 cut(s)	269, 488, 884, 1815
BssSI	CACGAG	1 cut(s)	2853
BssT1I	CCWWGG	3 cut(s)	2487, 2883, 3108
Bst2BI	CACGAG	1 cut(s)	2853
Bst2UI	CCWGG	2 cut(s)	1360, 1383
Bst6I	CTCTTC	7 cut(s)	281, 834, 1604, 1820, 1918, 2235, 2946
BstACI	GRCGYC	4 cut(s)	269, 488, 884, 1815
BstAFI	CTTAAG	1 cut(s)	1676
BstAPI	GCANNNNNTGC	2 cut(s)	869, 3136
BstDSI	CCRYGG	2 cut(s)	19, 2487
BstFNI	CGCG	1 cut(s)	127
BstH2I	RGCGCY	6 cut(s)	272, 389, 566, 584, 1439, 2560
BstHHI	GCGC	8 cut(s)	127, 153, 271, 388, 565, 583, 1438, 2559
BstMAI	GTCTC	7 cut(s)	33, 891, 1822, 2054, 2232, 2820, 2943
BstNI	CCWGG	2 cut(s)	1360, 1383
BstNSI	RCATGY	3 cut(s)	1909, 2291, 2984
BstSFI	CTRYAG	2 cut(s)	1878, 3524
BstUI	CGCG	1 cut(s)	127
BstV1I	GCAGC	9 cut(s)	121, 184, 235, 238, 859, 1237, 1588, 1864, 3123
BstV2I	GAAGAC	1 cut(s)	1313
BstXI	CCANNNNNNTGG	1 cut(s)	733
BsuI	GTATCC	4 cut(s)	395, 1595, 1861, 2104
BtgI	CCRYGG	2 cut(s)	19, 2487
BtgZI	GCGATG	1 cut(s)	2410
BtsI	GCAGTG	1 cut(s)	3521
BveI	ACCTGC	1 cut(s)	496
CaiI	CAGNNNCTG	2 cut(s)	1072, 3136
CciI	TCATGA	4 cut(s)	441, 1636, 2944, 3304
CfoI	GCGC	8 cut(s)	127, 153, 271, 388, 565, 583, 1438, 2559
Cfr10I	RCCGGY	2 cut(s)	1036, 1226
Cfr13I	GGNCC	9 cut(s)	4, 220, 326, 525, 661, 1979, 2548, 3044, 3421
Cfr9I	CCCGGG	1 cut(s)	3022
CpoI	CGGWCCG	1 cut(s)	220
CseI	GACGC	3 cut(s)	496, 539, 739
Csp6I	GTAC	5 cut(s)	1909, 2291, 2304, 2443, 2865
CspI	CGGWCCG	1 cut(s)	220
CviQI	GTAC	5 cut(s)	1909, 2291, 2304, 2443, 2865
DinI	GGCGCC	1 cut(s)	270
DraIII	CACNNNGTG	1 cut(s)	1841
EaeI	YGGCCR	6 cut(s)	432, 459, 553, 585, 2479, 3343
Eam1104I	CTCTTC	7 cut(s)	281, 834, 1604, 1820, 1918, 2235, 2946
EarI	CTCTTC	7 cut(s)	281, 834, 1604, 1820, 1918, 2235, 2946
Eco130I	CCWWGG	3 cut(s)	2487, 2883, 3108
Eco24I	GRGCYC	1 cut(s)	601
Eco32I	GATATC	1 cut(s)	2246
Eco47I	GGWCC	5 cut(s)	4, 220, 661, 1979, 2548
Eco57I	CTGAAG	3 cut(s)	410, 2027, 2240
Eco88I	CYCGRG	5 cut(s)	364, 491, 512, 1186, 3022
EcoO109I	RGGNCCY	1 cut(s)	3044
EcoRI	GAATTC	1 cut(s)	2074
EcoRII	CCWGG	2 cut(s)	1358, 1381
EcoRV	GATATC	1 cut(s)	2246
EcoT14I	CCWWGG	3 cut(s)	2487, 2883, 3108
EcoT38I	GRGCYC	1 cut(s)	601
EgeI	GGCGCC	1 cut(s)	270
EheI	GGCGCC	1 cut(s)	270
ErhI	CCWWGG	3 cut(s)	2487, 2883, 3108
Esp3I	CGTCTC	2 cut(s)	891, 2820
FalI	AAGNNNNNCTT	2 cut(s)	2988, 3020
FaqI	GGGAC	9 cut(s)	382, 823, 962, 1031, 1091, 1553, 2855, 3231, 3262
FauI	CCCGC	3 cut(s)	587, 717, 1854
FbaI	TGATCA	3 cut(s)	417, 3363, 3388
FriOI	GRGCYC	1 cut(s)	601
FspBI	CTAG	6 cut(s)	1317, 1838, 2249, 2274, 3269, 3501
GlaI	GCGC	8 cut(s)	126, 152, 270, 387, 564, 582, 1437, 2558
GsaI	CCCAGC	5 cut(s)	284, 1442, 3044, 3129, 3558
GsuI	CTGGAG	5 cut(s)	659, 755, 834, 2694, 2844
HaeII	RGCGCY	6 cut(s)	272, 389, 566, 584, 1439, 2560
HgaI	GACGC	3 cut(s)	496, 539, 739
HhaI	GCGC	8 cut(s)	127, 153, 271, 388, 565, 583, 1438, 2559
Hin1I	GRCGYC	4 cut(s)	269, 488, 884, 1815
Hin6I	GCGC	8 cut(s)	125, 151, 269, 386, 563, 581, 1436, 2557
HinP1I	GCGC	8 cut(s)	125, 151, 269, 386, 563, 581, 1436, 2557
HincII	GTYRAC	2 cut(s)	214, 3471
HindII	GTYRAC	2 cut(s)	214, 3471
HindIII	AAGCTT	2 cut(s)	41, 345
Hpy166II	GTNNAC	5 cut(s)	214, 798, 1388, 2548, 3471
Hpy8I	GTNNAC	5 cut(s)	214, 798, 1388, 2548, 3471
Hpy99I	CGWCG	5 cut(s)	79, 172, 533, 733, 2510
HpyCH4IV	ACGT	5 cut(s)	884, 1448, 1815, 2030, 2829
HpySE526I	ACGT	5 cut(s)	884, 1448, 1815, 2030, 2829
Hsp92I	GRCGYC	4 cut(s)	269, 488, 884, 1815
HspAI	GCGC	8 cut(s)	125, 151, 269, 386, 563, 581, 1436, 2557
KasI	GGCGCC	1 cut(s)	268
KpnI	GGTACC	1 cut(s)	2446
Ksp22I	TGATCA	3 cut(s)	417, 3363, 3388
Lsp1109I	GCAGC	9 cut(s)	121, 184, 235, 238, 859, 1237, 1588, 1864, 3123
LweI	GCATC	9 cut(s)	112, 1223, 2317, 2404, 2578, 2599, 2905, 3042, 3370
MaeI	CTAG	6 cut(s)	1317, 1838, 2249, 2274, 3269, 3501
MaeII	ACGT	5 cut(s)	884, 1448, 1815, 2030, 2829
MaeIII	GTNAC	6 cut(s)	880, 1345, 1772, 2114, 2687, 3580
MbiI	CCGCTC	2 cut(s)	523, 594
MfeI	CAATTG	1 cut(s)	1547
MhlI	GDGCHC	2 cut(s)	601, 2661
MlsI	TGGCCA	1 cut(s)	3345
MluNI	TGGCCA	1 cut(s)	3345
Mly113I	GGCGCC	1 cut(s)	269
MmeI	TCCRAC	2 cut(s)	450, 2732
Mox20I	TGGCCA	1 cut(s)	3345
MroXI	GAANNNNTTC	2 cut(s)	1377, 3000
MscI	TGGCCA	1 cut(s)	3345
MslI	CAYNNNNRTG	3 cut(s)	440, 698, 2685
Msp20I	TGGCCA	1 cut(s)	3345
MspA1I	CMGCKG	2 cut(s)	1069, 3326
MspCI	CTTAAG	1 cut(s)	1676
MunI	CAATTG	1 cut(s)	1547
Mva1269I	GAATGC	1 cut(s)	3101
MvaI	CCWGG	2 cut(s)	1360, 1383
MvnI	CGCG	1 cut(s)	127
NarI	GGCGCC	1 cut(s)	269
NciI	CCSGG	8 cut(s)	325, 431, 452, 665, 1015, 1313, 3023, 3024
NcoI	CCATGG	1 cut(s)	2487
NheI	GCTAGC	2 cut(s)	1316, 3268
NlaIV	GGNNCC	8 cut(s)	270, 322, 1035, 1126, 1664, 2444, 2550, 3045
NmeAIII	GCCGAG	4 cut(s)	233, 613, 1184, 2507
NmuCI	GTSAC	5 cut(s)	880, 1772, 2114, 2687, 3580
NspI	RCATGY	3 cut(s)	1909, 2291, 2984
PaeR7I	CTCGAG	1 cut(s)	512
PagI	TCATGA	4 cut(s)	441, 1636, 2944, 3304
PciI	ACATGT	3 cut(s)	1905, 2287, 2980
PcsI	WCGNNNNNNNCGW	1 cut(s)	2860
PctI	GAATGC	1 cut(s)	3101
PdmI	GAANNNNTTC	2 cut(s)	1377, 3000
PfeI	GAWTC	7 cut(s)	230, 313, 1354, 1934, 1989, 2704, 3436
PflMI	CCANNNNNTGG	2 cut(s)	3146, 3358
PluTI	GGCGCC	1 cut(s)	272
PscI	ACATGT	3 cut(s)	1905, 2287, 2980
PsiI	TTATAA	1 cut(s)	831
Psp6I	CCWGG	2 cut(s)	1358, 1381
PspFI	CCCAGC	5 cut(s)	280, 1438, 3040, 3125, 3554
PspGI	CCWGG	2 cut(s)	1358, 1381
PspN4I	GGNNCC	8 cut(s)	270, 322, 1035, 1126, 1664, 2444, 2550, 3045
PspPI	GGNCC	9 cut(s)	4, 220, 326, 525, 661, 1979, 2548, 3044, 3421
PstI	CTGCAG	2 cut(s)	1882, 3528
PstNI	CAGNNNCTG	2 cut(s)	1072, 3136
RsaI	GTAC	5 cut(s)	1910, 2292, 2305, 2444, 2866
RsaNI	GTAC	5 cut(s)	1909, 2291, 2304, 2443, 2865
RseI	CAYNNNNRTG	3 cut(s)	440, 698, 2685
Rsr2I	CGGWCCG	1 cut(s)	220
RsrII	CGGWCCG	1 cut(s)	220
Sau96I	GGNCC	9 cut(s)	4, 220, 326, 525, 661, 1979, 2548, 3044, 3421
SduI	GDGCHC	2 cut(s)	601, 2661
SfaNI	GCATC	9 cut(s)	112, 1223, 2317, 2404, 2578, 2599, 2905, 3042, 3370
SfcI	CTRYAG	2 cut(s)	1878, 3524
SfoI	GGCGCC	1 cut(s)	270
Sfr274I	CTCGAG	1 cut(s)	512
SinI	GGWCC	5 cut(s)	4, 220, 661, 1979, 2548
SlaI	CTCGAG	1 cut(s)	512
SmaI	CCCGGG	1 cut(s)	3024
SmiMI	CAYNNNNRTG	3 cut(s)	440, 698, 2685
SmlI	CTYRAG	5 cut(s)	512, 935, 1676, 2840, 3263
SmoI	CTYRAG	5 cut(s)	512, 935, 1676, 2840, 3263
SspDI	GGCGCC	1 cut(s)	268
SspMI	CTAG	6 cut(s)	1317, 1838, 2249, 2274, 3269, 3501
StyI	CCWWGG	3 cut(s)	2487, 2883, 3108
TaiI	ACGT	5 cut(s)	887, 1451, 1818, 2033, 2832
TaqII	GACCGA	2 cut(s)	208, 1982
TatI	WGTACW	1 cut(s)	1908
TauI	GCSGC	6 cut(s)	257, 274, 526, 555, 1072, 3326
TfiI	GAWTC	7 cut(s)	230, 313, 1354, 1934, 1989, 2704, 3436
TseFI	GTSAC	5 cut(s)	880, 1772, 2114, 2687, 3580
TseI	GCWGC	9 cut(s)	134, 172, 248, 251, 872, 1250, 1601, 1877, 3136
Tsp45I	GTSAC	5 cut(s)	880, 1772, 2114, 2687, 3580
TspGWI	ACGGA	6 cut(s)	156, 308, 627, 1067, 2298, 2889
TspMI	CCCGGG	1 cut(s)	3022
Van91I	CCANNNNNTGG	2 cut(s)	3146, 3358
Vha464I	CTTAAG	1 cut(s)	1676
VpaK11BI	GGWCC	5 cut(s)	4, 220, 661, 1979, 2548
XapI	RAATTY	4 cut(s)	568, 1376, 2074, 2953
XbaI	TCTAGA	1 cut(s)	2248
XceI	RCATGY	3 cut(s)	1909, 2291, 2984
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1243, 3211
XhoI	CTCGAG	1 cut(s)	512
XmaI	CCCGGG	1 cut(s)	3022
XmnI	GAANNNNTTC	2 cut(s)	1377, 3000
XspI	CTAG	6 cut(s)	1317, 1838, 2249, 2274, 3269, 3501
ZraI	GACGTC	2 cut(s)	885, 1816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.