Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

9699504 .. 9703336

Gene Structure 3833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1111400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2595 bp

ATGGACCGTTGCCTGAGCCACAGCGAGGCTCTCTCCCCGAAGCTCTCAATTTTCGGCAACTCCAAGCCCTATTCGTCGAAACCAAACAGCTTCCGCTTCGTCATTTCCTCAAAGAAACTCGGAGCGCGAAGCTCATCCTTACCTGCTCTCCGCCTCCGATCATTTCCGCCGCAGTTCGCCACTTTCCCCAACCGTACGAGTCACCGAGTCAACTCGGTCCGGTGCGACTCGGGTAATGGCGGAGCTGCTGCGGTGAAGGAAGGGGCGGTGCCAAAGCTGGGGAAGAGGACGGACATTAAGAAGATCATGATTCTCGGAGCCGTGCCGATTGTGATTAGGCAAGCTTGCGAGTTCGATTACTTGGGGACTCAGGCGTGCAAGGCGCTGAAGGAGGACGGATACGAGGTCATTTTGATCAATTCCAACCCGGCCACGATCATGACCGACCCGGACTTGGCCGACAAGACTTACATTACGCCCATGACTCCCGAGCTGGTTGAGCAAGTGCTCGAGAAGGAGCGGCCTGACGCTCTGTTGCCGACAATGGGAGGCCAGACGGCGCTGAATTTGGCCGTGGCACTGGCCGAGAGCGGGACTTTGGCCAAATACGGGGTCAAATTGATCGGAGCGAAGCTGGATGCCATCAAGAAAGCGGAGGACAGGGACTTGTTCAAGCAGGCCATGAAGAACATAGGTGTCAAGACGCCGCCATCTGGGATTGCGACTACTTTGGAGGAATGTATCAAAATTGCAAATGAGATTGGGGAGTTTCCATTGATTATCAGGCCAGCGTTTACTTTGGGAGGAACCGGAGGTGGCATTGCTTATAACAGAGAAGAATTTGAGGACATTTGTAAGGCGGGGATTGCGGCTAGTGTGACGTCTCAGGTTTTGGTTGAGAAGTCGTTGTTGGGATGGAAGGAGTATGAGCTTGAGGTGATGAGGGACTTGGCTGACAATGTAGTGATTATATGTTCCATTGAGAATATTGACCCAATGGGGGTTCATACAGGGGATTCGATCACTGTGGCCCCGGCTCAGACATTGACGGATAAGGAGTATCAGCGGTTGAGGGACTATTCCATTGCTATCATAAGGGAGATTGGAGTGGAATGTGGTGGTTCCAATGTGCAGTTTGCGGTTAATCCTGTCGATGGTGAGGTTATGGTGATTGAGATGAACCCGAGGGTGTCTCGGTCCTCGGCTTTGGCGTCCAAAGCTACTGGTTTTCCCATTGCGAAAATGGCTGCCAAGTTGTCGGTTGGGTATTCCTTGGATCAGATACCTAATGACATTACAAAGAAAACTCCAGCTAGCTTTGAGCCTTCCATAGATTATGTAGTGACCAAGATTCCCAGGTTTGCATTTGAAAAATTTCCAGGTTCACTACCAATATTGACTACACAAATGAAATCTGTTGGTGAGTCAATGGCACTGGGTCGCACATTTCAAGAGTCATTTCAGAAAGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTATGCTAAAATTAAGGAGCTTGACTGGGATTGGGAACAATTGAAGTATAGCCTCCGAGTCCCTAACCCAGACCCCATCCATGTCGTATATGCTGCAATGAAGAGGGGTATGAAGGTCGATGATATTCATGACTTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAGGAGCTGGTAGATGTGGAGCAATACCTCCTGGCTAGAAACTTATCTGATTTAACAAAGGATGACTTCTACGAAGTTAAAAAAAGAGGTTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCCTCTGAAAAAGATGTCAGGTTGAAGAGACTATCGTTAGGTATTGCTCCAGCATATAAGCGGGTGGATACTTGTGCTGCAAAGTTTGAGGCCAATACTCCGTTCATGTACTCTTCCTATGACTATGAGTGTGAATCAGCTCCCACCCAAAGTAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGCCAAGGGATCGAGTTTGACTACTGTTCAGGATATAAGACAATAATGATGAATTCAAATCCTGAAACAGTATCCACCGATTATGACACAAGTGACCGTCTATATTTTGAGCCACTAACTGTCGAAGACGTGCTGAACATAATTGATTTGGAAAGACCTGATGGCATCATTGTGCAGTTTGGAGGTCAAACACCGTTGAAGCTGTCACTTCCTATTCAGCAGTATCTAGATGAGAACAAGCCTAAATGTGCAAGTGGGAATGGACATGTACGCATTTGGGGTACTTCACCAGCTTCCATAGATGCTGCTGAGGACAGGGAGAAGTTCAATACAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGTATTGCCAAGAGTGAAGCTGATGTTGTTGCCATTGCGAAGGACATTGGTTACCCAATTGTTGTTCGGCCTTCGTATGTATTGGGTGGCCGAGCCATGGAGATTGTATATAGTGACGAAAAACTTGCGACCTACCTCGAAAATGCTGTTGAGATGGACCCAGAATGCCCTGTGTTGATTGACAAGTATCTGTCTGATGCCATTGAGATTGATGTCGATGCACTTGCTGACTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

865

Amino Acids

95.01

Weight (kDa)

5.36

Isoelectric Point (pI)

36.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	99 - 216	6.5e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	220 - 427	1.9e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	519 - 597	9.5e-25	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	650 - 764	1e-35	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	768 - 864	4.8e-16	Carbamoyl-phosphate synthase L chain, ATP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	828
AatII	GACGTC	1 cut(s)	884
Acc36I	ACCTGC	1 cut(s)	151
AccB1I	GGYRCC	1 cut(s)	268
AccBSI	CCGCTC	2 cut(s)	520, 591
AccII	CGCG	1 cut(s)	127
AclWI	GGATC	2 cut(s)	1284, 2006
AcoI	YGGCCR	7 cut(s)	429, 456, 570, 582, 600, 1990, 2446
AcsI	RAATTY	4 cut(s)	565, 839, 1373, 2041
AcuI	CTGAAG	1 cut(s)	407
AcyI	GRCGYC	3 cut(s)	704, 881, 1211
AfaI	GTAC	4 cut(s)	196, 1907, 2259, 2272
AflII	CTTAAG	1 cut(s)	1673
AflIII	ACRYGT	1 cut(s)	2254
AgsI	TTSAA	9 cut(s)	673, 1370, 1451, 1549, 1822, 2046, 2188, 2317, 2345
AjiI	CACGTC	1 cut(s)	2119
AjnI	CCWGG	3 cut(s)	1355, 1378, 1704
AjuI	GAANNNNNNNTTGG	4 cut(s)	893, 925, 1883, 1915
Alw21I	GWGCWC	1 cut(s)	510
Alw26I	GTCTC	3 cut(s)	888, 1197, 1819
AlwI	GGATC	2 cut(s)	1284, 2006
Ama87I	CYCGRG	4 cut(s)	229, 488, 509, 1183
ApeKI	GCWGC	6 cut(s)	245, 248, 1247, 1598, 1874, 2294
ApoI	RAATTY	4 cut(s)	565, 839, 1373, 2041
ArsI	GACNNNNNNTTYG	2 cut(s)	597, 629
Asp700I	GAANNNNTTC	1 cut(s)	1374
AspLEI	GCGC	3 cut(s)	127, 385, 562
AspS9I	GGNCC	6 cut(s)	4, 217, 1030, 1197, 1976, 2515
AsuC2I	CCSGG	3 cut(s)	428, 449, 1034
AsuHPI	GGTGA	7 cut(s)	194, 265, 949, 1169, 1180, 1433, 2268
AsuNHI	GCTAGC	1 cut(s)	1313
AvaI	CYCGRG	4 cut(s)	229, 488, 509, 1183
AvaII	GGWCC	5 cut(s)	4, 217, 1197, 1976, 2515
BalI	TGGCCA	2 cut(s)	602, 1992
BanI	GGYRCC	1 cut(s)	268
BbsI	GAAGAC	1 cut(s)	2121
Bbv12I	GWGCWC	1 cut(s)	510
BbvCI	CCTCAGC	1 cut(s)	2298
BbvI	GCAGC	6 cut(s)	232, 235, 1234, 1585, 1861, 2281
BccI	CCATC	8 cut(s)	650, 718, 909, 1148, 1494, 1589, 2144, 2506
BceAI	ACGGC	3 cut(s)	305, 557, 573
BcgI	CGANNNNNNTGC	3 cut(s)	2465, 2499, 2564
BciT130I	CCWGG	3 cut(s)	1357, 1380, 1706
BciVI	GTATCC	3 cut(s)	392, 1858, 2071
BclI	TGATCA	1 cut(s)	414
BcnI	CCSGG	3 cut(s)	428, 449, 1034
BcoDI	GTCTC	3 cut(s)	888, 1197, 1819
BfaI	CTAG	4 cut(s)	873, 1314, 1710, 2216
BfoI	RGCGCY	2 cut(s)	386, 563
BfrI	CTTAAG	1 cut(s)	1673
BfuAI	ACCTGC	1 cut(s)	151
BfuI	GTATCC	3 cut(s)	392, 1858, 2071
Bme1390I	CCNGG	6 cut(s)	428, 449, 1034, 1357, 1380, 1706
Bme18I	GGWCC	5 cut(s)	4, 217, 1197, 1976, 2515
BmeT110I	CYCGRG	4 cut(s)	229, 488, 509, 1183
BmgBI	CACGTC	1 cut(s)	2119
BmgT120I	GGNCC	6 cut(s)	4, 217, 1030, 1197, 1976, 2515
BmiI	GGNNCC	7 cut(s)	270, 319, 808, 1032, 1123, 1661, 2517
BmrFI	CCNGG	6 cut(s)	428, 449, 1034, 1357, 1380, 1706
BmrI	ACTGGG	2 cut(s)	1445, 1540
BmsI	GCATC	5 cut(s)	628, 2163, 2281, 2545, 2566
BmtI	GCTAGC	1 cut(s)	1317
BmuI	ACTGGG	2 cut(s)	1445, 1540
BpiI	GAAGAC	1 cut(s)	2121
BplI	GAGNNNNNCTC	4 cut(s)	17, 49, 1177, 1209
BpmI	CTGGAG	2 cut(s)	1293, 1830
Bpu10I	CCTNAGC	2 cut(s)	14, 2298
BpuEI	CTTGAG	2 cut(s)	953, 1660
BpuMI	CCSGG	3 cut(s)	428, 449, 1034
BsaHI	GRCGYC	3 cut(s)	704, 881, 1211
BsaJI	CCNNGG	8 cut(s)	573, 1032, 1184, 1200, 1272, 1355, 1993, 2454
BsaWI	WCCGGW	2 cut(s)	219, 809
BsaXI	ACNNNNNCTCC	2 cut(s)	132, 162
Bse1I	ACTGG	4 cut(s)	585, 1228, 1440, 1535
Bse3DI	GCAATG	5 cut(s)	819, 1083, 1233, 1608, 2391
BseBI	CCWGG	3 cut(s)	1357, 1380, 1706
BseDI	CCNNGG	8 cut(s)	573, 1032, 1184, 1200, 1272, 1355, 1993, 2454
BseGI	GGATG	6 cut(s)	134, 643, 920, 1505, 1581, 1741
BseMI	GCAATG	5 cut(s)	819, 1083, 1233, 1608, 2391
BseMII	CTCAG	5 cut(s)	5, 383, 899, 1052, 2289
BseNI	ACTGG	4 cut(s)	585, 1228, 1440, 1535
BseXI	GCAGC	6 cut(s)	232, 235, 1234, 1585, 1861, 2281
BseYI	CCCAGC	1 cut(s)	277
BsgI	GTGCAG	2 cut(s)	1151, 2183
Bsh1236I	CGCG	1 cut(s)	127
BshNI	GGYRCC	1 cut(s)	268
BsiHKAI	GWGCWC	1 cut(s)	510
BsiHKCI	CYCGRG	4 cut(s)	229, 488, 509, 1183
BsiSI	CCGG	5 cut(s)	220, 428, 449, 810, 1034
BsiWI	CGTACG	1 cut(s)	194
BslFI	GGGAC	6 cut(s)	379, 607, 677, 959, 1088, 1550
BsmAI	GTCTC	3 cut(s)	888, 1197, 1819
BsmBI	CGTCTC	1 cut(s)	888
BsmFI	GGGAC	6 cut(s)	379, 607, 677, 959, 1088, 1550
BsmI	GAATGC	1 cut(s)	2528
BsoBI	CYCGRG	4 cut(s)	229, 488, 509, 1183
Bsp1286I	GDGCHC	1 cut(s)	510
Bsp143I	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
Bsp19I	CCATGG	1 cut(s)	2454
BspCNI	CTCAG	5 cut(s)	6, 382, 898, 1051, 2290
BspFNI	CGCG	1 cut(s)	127
BspHI	TCATGA	3 cut(s)	306, 438, 1633
BspLI	GGNNCC	7 cut(s)	270, 319, 808, 1032, 1123, 1661, 2517
BspMI	ACCTGC	1 cut(s)	151
BspOI	GCTAGC	1 cut(s)	1317
BspPI	GGATC	2 cut(s)	1284, 2006
BspT107I	GGYRCC	1 cut(s)	268
BspTI	CTTAAG	1 cut(s)	1673
BsrBI	CCGCTC	2 cut(s)	520, 591
BsrDI	GCAATG	5 cut(s)	819, 1083, 1233, 1608, 2391
BsrI	ACTGG	4 cut(s)	585, 1228, 1440, 1535
BssECI	CCNNGG	8 cut(s)	573, 1032, 1184, 1200, 1272, 1355, 1993, 2454
BssMI	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
BssNI	GRCGYC	3 cut(s)	704, 881, 1211
BssT1I	CCWWGG	3 cut(s)	1272, 1993, 2454
Bst2UI	CCWGG	3 cut(s)	1357, 1380, 1706
Bst4CI	ACNGT	8 cut(s)	8, 194, 1027, 2015, 2059, 2087, 2110, 2184
Bst6I	CTCTTC	4 cut(s)	278, 1601, 1817, 1915
BstACI	GRCGYC	3 cut(s)	704, 881, 1211
BstAFI	CTTAAG	1 cut(s)	1673
BstC8I	GCNNGC	6 cut(s)	342, 346, 376, 678, 789, 1315
BstDEI	CTNAG	6 cut(s)	14, 369, 885, 1038, 2298, 2592
BstDSI	CCRYGG	2 cut(s)	573, 2454
BstEII	GGTNACC	1 cut(s)	2408
BstF5I	GGATG	6 cut(s)	134, 643, 920, 1505, 1581, 1741
BstFNI	CGCG	1 cut(s)	127
BstH2I	RGCGCY	2 cut(s)	386, 563
BstHHI	GCGC	3 cut(s)	127, 385, 562
BstKTI	GATC	9 cut(s)	161, 306, 417, 438, 624, 1023, 1279, 1478, 2001
BstMAI	GTCTC	3 cut(s)	888, 1197, 1819
BstMBI	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
BstMWI	GCNNNNNNNGC	6 cut(s)	380, 499, 866, 1217, 1244, 1678
BstNI	CCWGG	3 cut(s)	1357, 1380, 1706
BstNSI	RCATGY	1 cut(s)	2258
BstPI	GGTNACC	1 cut(s)	2408
BstSCI	CCNGG	6 cut(s)	426, 447, 1032, 1355, 1378, 1704
BstUI	CGCG	1 cut(s)	127
BstV1I	GCAGC	6 cut(s)	232, 235, 1234, 1585, 1861, 2281
BstV2I	GAAGAC	1 cut(s)	2121
BsuI	GTATCC	3 cut(s)	392, 1858, 2071
BtgI	CCRYGG	2 cut(s)	573, 2454
BtrI	CACGTC	1 cut(s)	2119
BtsCI	GGATG	6 cut(s)	134, 643, 920, 1505, 1581, 1741
BtsIMutI	CAGTG	4 cut(s)	578, 1023, 1433, 1774
BveI	ACCTGC	1 cut(s)	151
Cac8I	GCNNGC	6 cut(s)	342, 346, 376, 678, 789, 1315
CciI	TCATGA	3 cut(s)	306, 438, 1633
CfoI	GCGC	3 cut(s)	127, 385, 562
Cfr13I	GGNCC	6 cut(s)	4, 217, 1030, 1197, 1976, 2515
CpoI	CGGWCCG	1 cut(s)	217
CseI	GACGC	3 cut(s)	536, 712, 1200
Csp6I	GTAC	4 cut(s)	195, 1906, 2258, 2271
CspI	CGGWCCG	1 cut(s)	217
CviAII	CATG	9 cut(s)	307, 439, 481, 682, 1586, 1634, 1903, 2255, 2455
CviQI	GTAC	4 cut(s)	195, 1906, 2258, 2271
DdeI	CTNAG	6 cut(s)	14, 369, 885, 1038, 2298, 2592
DpnI	GATC	9 cut(s)	160, 305, 416, 437, 623, 1022, 1278, 1477, 2000
DpnII	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
EaeI	YGGCCR	7 cut(s)	429, 456, 570, 582, 600, 1990, 2446
Eam1104I	CTCTTC	4 cut(s)	278, 1601, 1817, 1915
EarI	CTCTTC	4 cut(s)	278, 1601, 1817, 1915
EciI	GGCGGA	3 cut(s)	140, 156, 255
Eco130I	CCWWGG	3 cut(s)	1272, 1993, 2454
Eco47I	GGWCC	5 cut(s)	4, 217, 1197, 1976, 2515
Eco57I	CTGAAG	1 cut(s)	407
Eco88I	CYCGRG	4 cut(s)	229, 488, 509, 1183
Eco91I	GGTNACC	1 cut(s)	2408
EcoO65I	GGTNACC	1 cut(s)	2408
EcoRI	GAATTC	1 cut(s)	2041
EcoRII	CCWGG	3 cut(s)	1355, 1378, 1704
EcoT14I	CCWWGG	3 cut(s)	1272, 1993, 2454
ErhI	CCWWGG	3 cut(s)	1272, 1993, 2454
Esp3I	CGTCTC	1 cut(s)	888
FaeI	CATG	9 cut(s)	310, 442, 484, 685, 1589, 1637, 1906, 2258, 2458
FalI	AAGNNNNNCTT	2 cut(s)	1724, 1756
FaqI	GGGAC	6 cut(s)	379, 607, 677, 959, 1088, 1550
FatI	CATG	9 cut(s)	306, 438, 480, 681, 1585, 1633, 1902, 2254, 2454
FauI	CCCGC	3 cut(s)	584, 853, 1851
FbaI	TGATCA	1 cut(s)	414
FokI	GGATG	6 cut(s)	121, 650, 927, 1512, 1568, 1748
FspBI	CTAG	4 cut(s)	873, 1314, 1710, 2216
GlaI	GCGC	3 cut(s)	126, 384, 561
GsaI	CCCAGC	1 cut(s)	281
GsuI	CTGGAG	2 cut(s)	1293, 1830
HaeII	RGCGCY	2 cut(s)	386, 563
HapII	CCGG	5 cut(s)	220, 428, 449, 810, 1034
HgaI	GACGC	3 cut(s)	536, 712, 1200
HhaI	GCGC	3 cut(s)	127, 385, 562
Hin1I	GRCGYC	3 cut(s)	704, 881, 1211
Hin1II	CATG	9 cut(s)	310, 442, 484, 685, 1589, 1637, 1906, 2258, 2458
Hin6I	GCGC	3 cut(s)	125, 383, 560
HinP1I	GCGC	3 cut(s)	125, 383, 560
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HindIII	AAGCTT	1 cut(s)	342
HpaII	CCGG	5 cut(s)	220, 428, 449, 810, 1034
HphI	GGTGA	7 cut(s)	194, 265, 949, 1169, 1180, 1433, 2268
Hpy166II	GTNNAC	3 cut(s)	211, 795, 1385
Hpy8I	GTNNAC	3 cut(s)	211, 795, 1385
Hpy99I	CGWCG	1 cut(s)	79
HpyCH4III	ACNGT	8 cut(s)	8, 194, 1027, 2015, 2059, 2087, 2110, 2184
HpyCH4IV	ACGT	2 cut(s)	881, 2118
HpyCH4V	TGCA	9 cut(s)	378, 752, 1132, 1364, 1601, 1877, 2164, 2240, 2579
HpyF10VI	GCNNNNNNNGC	6 cut(s)	380, 499, 866, 1217, 1244, 1678
HpyF3I	CTNAG	6 cut(s)	14, 369, 885, 1038, 2298, 2592
HpySE526I	ACGT	2 cut(s)	881, 2118
Hsp92I	GRCGYC	3 cut(s)	704, 881, 1211
Hsp92II	CATG	9 cut(s)	310, 442, 484, 685, 1589, 1637, 1906, 2258, 2458
HspAI	GCGC	3 cut(s)	125, 383, 560
Ksp22I	TGATCA	1 cut(s)	414
Kzo9I	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
Lsp1109I	GCAGC	6 cut(s)	232, 235, 1234, 1585, 1861, 2281
LweI	GCATC	5 cut(s)	628, 2163, 2281, 2545, 2566
MaeI	CTAG	4 cut(s)	873, 1314, 1710, 2216
MaeII	ACGT	2 cut(s)	881, 2118
MaeIII	GTNAC	8 cut(s)	200, 877, 1342, 1769, 2081, 2193, 2408, 2471
MalI	GATC	9 cut(s)	160, 305, 416, 437, 623, 1022, 1278, 1477, 2000
MbiI	CCGCTC	2 cut(s)	520, 591
MboI	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
MboII	GAAGA	8 cut(s)	295, 313, 697, 848, 1618, 1834, 1902, 2126
MfeI	CAATTG	2 cut(s)	1544, 2415
MhlI	GDGCHC	1 cut(s)	510
MlsI	TGGCCA	2 cut(s)	602, 1992
MluNI	TGGCCA	2 cut(s)	602, 1992
MlyI	GAGTC	9 cut(s)	208, 216, 221, 361, 478, 1433, 1463, 1572, 2582
MmeI	TCCRAC	1 cut(s)	447
Mox20I	TGGCCA	2 cut(s)	602, 1992
MroXI	GAANNNNTTC	1 cut(s)	1374
MscI	TGGCCA	2 cut(s)	602, 1992
MseI	TTAA	8 cut(s)	297, 1143, 1518, 1674, 1727, 1752, 2328, 2336
MslI	CAYNNNNRTG	2 cut(s)	437, 2159
Msp20I	TGGCCA	2 cut(s)	602, 1992
MspA1I	CMGCKG	1 cut(s)	1066
MspCI	CTTAAG	1 cut(s)	1673
MspI	CCGG	5 cut(s)	220, 428, 449, 810, 1034
MspR9I	CCNGG	6 cut(s)	428, 449, 1034, 1357, 1380, 1706
MunI	CAATTG	2 cut(s)	1544, 2415
Mva1269I	GAATGC	1 cut(s)	2528
MvaI	CCWGG	3 cut(s)	1357, 1380, 1706
MvnI	CGCG	1 cut(s)	127
MwoI	GCNNNNNNNGC	6 cut(s)	380, 499, 866, 1217, 1244, 1678
NciI	CCSGG	3 cut(s)	428, 449, 1034
NcoI	CCATGG	1 cut(s)	2454
NdeII	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
NheI	GCTAGC	1 cut(s)	1313
NlaIII	CATG	9 cut(s)	310, 442, 484, 685, 1589, 1637, 1906, 2258, 2458
NlaIV	GGNNCC	7 cut(s)	270, 319, 808, 1032, 1123, 1661, 2517
NmeAIII	GCCGAG	3 cut(s)	610, 1181, 2474
NmuCI	GTSAC	7 cut(s)	200, 877, 1342, 1769, 2081, 2193, 2471
NspI	RCATGY	1 cut(s)	2258
PaeR7I	CTCGAG	1 cut(s)	509
PagI	TCATGA	3 cut(s)	306, 438, 1633
PciI	ACATGT	1 cut(s)	2254
PcsI	WCGNNNNNNNCGW	1 cut(s)	1208
PctI	GAATGC	1 cut(s)	2528
PdmI	GAANNNNTTC	1 cut(s)	1374
PfeI	GAWTC	4 cut(s)	310, 1016, 1351, 1931
Pfl23II	CGTACG	1 cut(s)	194
PleI	GAGTC	9 cut(s)	207, 215, 221, 361, 478, 1432, 1462, 1571, 2582
PpsI	GAGTC	9 cut(s)	207, 215, 221, 361, 478, 1432, 1462, 1571, 2582
PscI	ACATGT	1 cut(s)	2254
PsiI	TTATAA	1 cut(s)	828
Psp6I	CCWGG	3 cut(s)	1355, 1378, 1704
PspEI	GGTNACC	1 cut(s)	2408
PspFI	CCCAGC	1 cut(s)	277
PspGI	CCWGG	3 cut(s)	1355, 1378, 1704
PspLI	CGTACG	1 cut(s)	194
PspN4I	GGNNCC	7 cut(s)	270, 319, 808, 1032, 1123, 1661, 2517
PspPI	GGNCC	6 cut(s)	4, 217, 1030, 1197, 1976, 2515
RsaI	GTAC	4 cut(s)	196, 1907, 2259, 2272
RsaNI	GTAC	4 cut(s)	195, 1906, 2258, 2271
RseI	CAYNNNNRTG	2 cut(s)	437, 2159
Rsr2I	CGGWCCG	1 cut(s)	217
RsrII	CGGWCCG	1 cut(s)	217
SaqAI	TTAA	8 cut(s)	297, 1143, 1518, 1674, 1727, 1752, 2328, 2336
Sau3AI	GATC	9 cut(s)	158, 303, 414, 435, 621, 1020, 1276, 1475, 1998
Sau96I	GGNCC	6 cut(s)	4, 217, 1030, 1197, 1976, 2515
SchI	GAGTC	9 cut(s)	208, 216, 221, 361, 478, 1433, 1463, 1572, 2582
ScrFI	CCNGG	6 cut(s)	428, 449, 1034, 1357, 1380, 1706
SduI	GDGCHC	1 cut(s)	510
SfaNI	GCATC	5 cut(s)	628, 2163, 2281, 2545, 2566
Sfr274I	CTCGAG	1 cut(s)	509
SinI	GGWCC	5 cut(s)	4, 217, 1197, 1976, 2515
SlaI	CTCGAG	1 cut(s)	509
SmiMI	CAYNNNNRTG	2 cut(s)	437, 2159
SmlI	CTYRAG	4 cut(s)	509, 932, 1639, 1673
SmoI	CTYRAG	4 cut(s)	509, 932, 1639, 1673
SspI	AATATT	2 cut(s)	988, 1395
SspMI	CTAG	4 cut(s)	873, 1314, 1710, 2216
StyD4I	CCNGG	6 cut(s)	426, 447, 1032, 1355, 1378, 1704
StyI	CCWWGG	3 cut(s)	1272, 1993, 2454
TaaI	ACNGT	8 cut(s)	8, 194, 1027, 2015, 2059, 2087, 2110, 2184
TaiI	ACGT	2 cut(s)	884, 2121
TaqII	GACCGA	3 cut(s)	205, 458, 1185
TatI	WGTACW	1 cut(s)	1905
TauI	GCSGC	4 cut(s)	172, 523, 709, 872
TfiI	GAWTC	4 cut(s)	310, 1016, 1351, 1931
Tru1I	TTAA	8 cut(s)	297, 1143, 1518, 1674, 1727, 1752, 2328, 2336
Tru9I	TTAA	8 cut(s)	297, 1143, 1518, 1674, 1727, 1752, 2328, 2336
TscAI	CASTG	4 cut(s)	585, 1030, 1440, 1774
TseFI	GTSAC	7 cut(s)	200, 877, 1342, 1769, 2081, 2193, 2471
TseI	GCWGC	6 cut(s)	245, 248, 1247, 1598, 1874, 2294
Tsp45I	GTSAC	7 cut(s)	200, 877, 1342, 1769, 2081, 2193, 2471
TspGWI	ACGGA	4 cut(s)	305, 411, 1064, 1887
TspRI	CASTG	4 cut(s)	585, 1030, 1440, 1774
Vha464I	CTTAAG	1 cut(s)	1673
VpaK11BI	GGWCC	5 cut(s)	4, 217, 1197, 1976, 2515
XapI	RAATTY	4 cut(s)	565, 839, 1373, 2041
XbaI	TCTAGA	1 cut(s)	2215
XceI	RCATGY	1 cut(s)	2258
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	509
XmnI	GAANNNNTTC	1 cut(s)	1374
XspI	CTAG	4 cut(s)	873, 1314, 1710, 2216
ZraI	GACGTC	1 cut(s)	882

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.