Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

24693184 .. 24694071

Gene Structure 888 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1166200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 888 bp

ATGGACATTAAGAAGATCATGATTCTCGGAGCCGGGCCGATTGTGATTGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAGGCGTGCAAGGCGCTGAAGGAGGACGGATACGAGGTTGTTTTGATTAATTCCAACCCGGCCACGATCATGATCGACCCGAACTTGGCCGACAAGACTTACATTACGCCCATGACTTCCGAGCTAGTTGAGCAGGTGCTCGAGAATGAGCAGCCTGACGCTCTGTTGCTGACAATGAGAGGCCAGACGGCGCTGAATTTGGCCGTGGCACTGGCCAAGAGCGGGACTTTGGCCAAATATGGGGTCGAATTGATCGGAGCAAAGTTGGATGCCATCAAGAAAGCAGAAGACATGGACTTGTTCAAGCAGGCCATGAAGAACATAGGTATCAAGACGTCGCCATCTGGGATTGCGACTACTTTAGAGGAATGTATCAAAATTGTGAATGAGATTAGGGAGTTTCCATTGATTATCAGGCCGGCATTTACTTTGGGAGGAACCGGAGGTGGCACTGCTTATAACAAAGAAAAATTTGAGGACATTTGTAAGGCAGTGATTGCAGCTAGTGTGACATCTCAGGTTTTGGTTGAGAAGTCGTTGTTGGGATGGAAGGAGTATGAGCTTAAGGTGATTAGGGACTTGGCTGACAATGTAGTGATTATATGTTTCATTGAGAATATTGACCCAATGGGGGTTCATACAAGGGATACTATCACTGTGGCCCCGGCTCAGACTTTGATGGATAAGGAGTATCAGCGGCTCAGGGACTATTCCATTGCTATCATAAGGGAGATTGGAGTGGAATGTGGTGGTTCCAATGTGCAGTTTGTGGTTAATCCTGTCGATGGTGAGGTTATGGTGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

296

Amino Acids

32.09

Weight (kDa)

4.78

Isoelectric Point (pI)

26.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	3 - 120	3.4e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	124 - 293	1.1e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	540
AarI	CACCTGC	1 cut(s)	205
AatII	GACGTC	1 cut(s)	419
Acc36I	ACCTGC	1 cut(s)	205
AccBSI	CCGCTC	1 cut(s)	303
AciI	CCGC	2 cut(s)	303, 778
AcoI	YGGCCR	5 cut(s)	141, 168, 282, 294, 312
AcsI	RAATTY	2 cut(s)	277, 551
AcuI	CTGAAG	1 cut(s)	119
AcyI	GRCGYC	1 cut(s)	416
AfiI	CCNNNNNNNGG	5 cut(s)	166, 303, 321, 712, 866
AflII	CTTAAG	1 cut(s)	644
AgsI	TTSAA	1 cut(s)	385
AjuI	GAANNNNNNNTTGG	2 cut(s)	605, 637
AluBI	AGCT	4 cut(s)	56, 205, 584, 643
AluI	AGCT	4 cut(s)	56, 205, 584, 643
Alw21I	GWGCWC	1 cut(s)	222
Ama87I	CYCGRG	2 cut(s)	73, 221
ApeKI	GCWGC	2 cut(s)	232, 581
ApoI	RAATTY	2 cut(s)	277, 551
ArsI	GACNNNNNNTTYG	2 cut(s)	309, 341
AseI	ATTAAT	1 cut(s)	129
AspLEI	GCGC	2 cut(s)	97, 274
AspS9I	GGNCC	2 cut(s)	35, 742
AsuC2I	CCSGG	3 cut(s)	34, 140, 746
AsuHPI	GGTGA	2 cut(s)	661, 881
AvaI	CYCGRG	2 cut(s)	73, 221
BaeI	ACNNNNGTAYC	2 cut(s)	391, 424
BalI	TGGCCA	2 cut(s)	296, 314
BbsI	GAAGAC	1 cut(s)	375
Bbv12I	GWGCWC	1 cut(s)	222
BbvI	GCAGC	2 cut(s)	244, 593
BccI	CCATC	5 cut(s)	362, 430, 621, 754, 860
BceAI	ACGGC	2 cut(s)	269, 285
BciVI	GTATCC	2 cut(s)	104, 721
BcnI	CCSGG	3 cut(s)	34, 140, 746
BfaI	CTAG	2 cut(s)	206, 585
BfoI	RGCGCY	2 cut(s)	98, 275
BfrI	CTTAAG	1 cut(s)	644
BfuAI	ACCTGC	1 cut(s)	205
BfuI	GTATCC	2 cut(s)	104, 721
BisI	GCNGC	3 cut(s)	233, 582, 779
BlsI	GCNGC	3 cut(s)	234, 583, 780
Bme1390I	CCNGG	3 cut(s)	34, 140, 746
BmeT110I	CYCGRG	2 cut(s)	73, 221
BmgT120I	GGNCC	2 cut(s)	35, 742
BmiI	GGNNCC	4 cut(s)	31, 520, 744, 835
BmrFI	CCNGG	3 cut(s)	34, 140, 746
BmsI	GCATC	1 cut(s)	340
BpiI	GAAGAC	1 cut(s)	375
Bpu10I	CCTNAGC	1 cut(s)	782
BpuMI	CCSGG	3 cut(s)	34, 140, 746
BsaBI	GATNNNNATC	1 cut(s)	152
BsaHI	GRCGYC	1 cut(s)	416
BsaJI	CCNNGG	2 cut(s)	285, 744
BsaWI	WCCGGW	1 cut(s)	521
Bsc4I	CCNNNNNNNGG	5 cut(s)	166, 303, 321, 712, 866
Bse118I	RCCGGY	1 cut(s)	499
Bse1I	ACTGG	1 cut(s)	297
Bse3DI	GCAATG	1 cut(s)	795
Bse8I	GATNNNNATC	1 cut(s)	152
BseDI	CCNNGG	2 cut(s)	285, 744
BseGI	GGATG	2 cut(s)	355, 632
BseJI	GATNNNNATC	1 cut(s)	152
BseLI	CCNNNNNNNGG	5 cut(s)	166, 303, 321, 712, 866
BseMI	GCAATG	1 cut(s)	795
BseMII	CTCAG	4 cut(s)	95, 611, 764, 796
BseNI	ACTGG	1 cut(s)	297
BseXI	GCAGC	2 cut(s)	244, 593
BsgI	GTGCAG	1 cut(s)	863
BsiHKAI	GWGCWC	1 cut(s)	222
BsiHKCI	CYCGRG	2 cut(s)	73, 221
BsiSI	CCGG	5 cut(s)	33, 140, 500, 522, 746
BslFI	GGGAC	4 cut(s)	91, 319, 671, 800
BslI	CCNNNNNNNGG	5 cut(s)	166, 303, 321, 712, 866
BsmFI	GGGAC	4 cut(s)	91, 319, 671, 800
BsoBI	CYCGRG	2 cut(s)	73, 221
Bsp1286I	GDGCHC	1 cut(s)	222
Bsp143I	GATC	4 cut(s)	15, 147, 153, 333
BspACI	CCGC	2 cut(s)	303, 778
BspCNI	CTCAG	4 cut(s)	94, 610, 763, 795
BspHI	TCATGA	2 cut(s)	18, 150
BspLI	GGNNCC	4 cut(s)	31, 520, 744, 835
BspMI	ACCTGC	1 cut(s)	205
BspTI	CTTAAG	1 cut(s)	644
BsrBI	CCGCTC	1 cut(s)	303
BsrDI	GCAATG	1 cut(s)	795
BsrFI	RCCGGY	1 cut(s)	499
BsrI	ACTGG	1 cut(s)	297
BssAI	RCCGGY	1 cut(s)	499
BssECI	CCNNGG	2 cut(s)	285, 744
BssMI	GATC	4 cut(s)	15, 147, 153, 333
BssNI	GRCGYC	1 cut(s)	416
Bst4CI	ACNGT	1 cut(s)	739
BstACI	GRCGYC	1 cut(s)	416
BstAFI	CTTAAG	1 cut(s)	644
BstAPI	GCANNNNNTGC	1 cut(s)	578
BstC8I	GCNNGC	5 cut(s)	54, 58, 88, 390, 501
BstDEI	CTNAG	4 cut(s)	81, 597, 750, 782
BstDSI	CCRYGG	1 cut(s)	285
BstF5I	GGATG	2 cut(s)	355, 632
BstH2I	RGCGCY	2 cut(s)	98, 275
BstHHI	GCGC	2 cut(s)	97, 274
BstKTI	GATC	4 cut(s)	18, 150, 156, 336
BstMBI	GATC	4 cut(s)	15, 147, 153, 333
BstMWI	GCNNNNNNNGC	3 cut(s)	92, 211, 578
BstSCI	CCNGG	3 cut(s)	32, 138, 744
BstV1I	GCAGC	2 cut(s)	244, 593
BstV2I	GAAGAC	1 cut(s)	375
BsuI	GTATCC	2 cut(s)	104, 721
BtgI	CCRYGG	1 cut(s)	285
BtsCI	GGATG	2 cut(s)	355, 632
BtsI	GCAGTG	2 cut(s)	531, 579
BtsIMutI	CAGTG	4 cut(s)	290, 531, 579, 735
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	5 cut(s)	54, 58, 88, 390, 501
CciI	TCATGA	2 cut(s)	18, 150
CfoI	GCGC	2 cut(s)	97, 274
Cfr10I	RCCGGY	1 cut(s)	499
Cfr13I	GGNCC	2 cut(s)	35, 742
CseI	GACGC	1 cut(s)	248
CviAII	CATG	5 cut(s)	19, 151, 193, 373, 394
DdeI	CTNAG	4 cut(s)	81, 597, 750, 782
DpnI	GATC	4 cut(s)	17, 149, 155, 335
DpnII	GATC	4 cut(s)	15, 147, 153, 333
EaeI	YGGCCR	5 cut(s)	141, 168, 282, 294, 312
Eco57I	CTGAAG	1 cut(s)	119
Eco88I	CYCGRG	2 cut(s)	73, 221
FaeI	CATG	5 cut(s)	22, 154, 196, 376, 397
FaqI	GGGAC	4 cut(s)	91, 319, 671, 800
FatI	CATG	5 cut(s)	18, 150, 192, 372, 393
FauI	CCCGC	1 cut(s)	296
Fnu4HI	GCNGC	3 cut(s)	233, 582, 779
FokI	GGATG	2 cut(s)	362, 639
Fsp4HI	GCNGC	3 cut(s)	233, 582, 779
FspBI	CTAG	2 cut(s)	206, 585
GlaI	GCGC	2 cut(s)	96, 273
GluI	GCNGC	3 cut(s)	233, 582, 779
HaeII	RGCGCY	2 cut(s)	98, 275
HapII	CCGG	5 cut(s)	33, 140, 500, 522, 746
HgaI	GACGC	1 cut(s)	248
HhaI	GCGC	2 cut(s)	97, 274
Hin1I	GRCGYC	1 cut(s)	416
Hin1II	CATG	5 cut(s)	22, 154, 196, 376, 397
Hin6I	GCGC	2 cut(s)	95, 272
HinP1I	GCGC	2 cut(s)	95, 272
HindIII	AAGCTT	1 cut(s)	54
HinfI	GANTC	2 cut(s)	22, 79
HpaII	CCGG	5 cut(s)	33, 140, 500, 522, 746
HphI	GGTGA	2 cut(s)	661, 881
Hpy188I	TCNGA	4 cut(s)	29, 202, 338, 753
Hpy188III	TCNNGA	5 cut(s)	19, 151, 223, 358, 412
Hpy99I	CGWCG	1 cut(s)	421
HpyAV	CCTTC	2 cut(s)	94, 625
HpyCH4III	ACNGT	1 cut(s)	739
HpyCH4IV	ACGT	1 cut(s)	416
HpyCH4V	TGCA	3 cut(s)	90, 581, 844
HpyF10VI	GCNNNNNNNGC	3 cut(s)	92, 211, 578
HpyF3I	CTNAG	4 cut(s)	81, 597, 750, 782
HpySE526I	ACGT	1 cut(s)	416
Hsp92I	GRCGYC	1 cut(s)	416
Hsp92II	CATG	5 cut(s)	22, 154, 196, 376, 397
HspAI	GCGC	2 cut(s)	95, 272
KroI	GCCGGC	1 cut(s)	499
KroNI	GCCGGC	1 cut(s)	501
Kzo9I	GATC	4 cut(s)	15, 147, 153, 333
LmnI	GCTCC	2 cut(s)	29, 338
Lsp1109I	GCAGC	2 cut(s)	244, 593
LweI	GCATC	1 cut(s)	340
MaeI	CTAG	2 cut(s)	206, 585
MaeII	ACGT	1 cut(s)	416
MaeIII	GTNAC	1 cut(s)	589
MalI	GATC	4 cut(s)	17, 149, 155, 335
MbiI	CCGCTC	1 cut(s)	303
MboI	GATC	4 cut(s)	15, 147, 153, 333
MboII	GAAGA	3 cut(s)	25, 380, 409
MhlI	GDGCHC	1 cut(s)	222
MlsI	TGGCCA	2 cut(s)	296, 314
MluCI	AATT	5 cut(s)	130, 277, 329, 459, 551
MluNI	TGGCCA	2 cut(s)	296, 314
MlyI	GAGTC	1 cut(s)	73
MmeI	TCCRAC	2 cut(s)	159, 327
MnlI	CCTC	8 cut(s)	97, 109, 254, 439, 509, 518, 550, 865
Mox20I	TGGCCA	2 cut(s)	296, 314
MroNI	GCCGGC	1 cut(s)	499
MscI	TGGCCA	2 cut(s)	296, 314
MseI	TTAA	4 cut(s)	9, 129, 645, 855
MslI	CAYNNNNRTG	1 cut(s)	149
Msp20I	TGGCCA	2 cut(s)	296, 314
MspA1I	CMGCKG	1 cut(s)	778
MspCI	CTTAAG	1 cut(s)	644
MspI	CCGG	5 cut(s)	33, 140, 500, 522, 746
MspR9I	CCNGG	3 cut(s)	34, 140, 746
MwoI	GCNNNNNNNGC	3 cut(s)	92, 211, 578
NaeI	GCCGGC	1 cut(s)	501
NciI	CCSGG	3 cut(s)	34, 140, 746
NdeII	GATC	4 cut(s)	15, 147, 153, 333
NgoMIV	GCCGGC	1 cut(s)	499
NlaIII	CATG	5 cut(s)	22, 154, 196, 376, 397
NlaIV	GGNNCC	4 cut(s)	31, 520, 744, 835
NmuCI	GTSAC	1 cut(s)	589
PaeR7I	CTCGAG	1 cut(s)	221
PagI	TCATGA	2 cut(s)	18, 150
PaqCI	CACCTGC	1 cut(s)	205
PdiI	GCCGGC	1 cut(s)	501
PfeI	GAWTC	1 cut(s)	22
PkrI	GCNGC	3 cut(s)	234, 583, 780
PleI	GAGTC	1 cut(s)	73
PpsI	GAGTC	1 cut(s)	73
PshBI	ATTAAT	1 cut(s)	129
PsiI	TTATAA	1 cut(s)	540
PspN4I	GGNNCC	4 cut(s)	31, 520, 744, 835
PspPI	GGNCC	2 cut(s)	35, 742
RseI	CAYNNNNRTG	1 cut(s)	149
SaqAI	TTAA	4 cut(s)	9, 129, 645, 855
SatI	GCNGC	3 cut(s)	233, 582, 779
Sau3AI	GATC	4 cut(s)	15, 147, 153, 333
Sau96I	GGNCC	2 cut(s)	35, 742
SchI	GAGTC	1 cut(s)	73
ScrFI	CCNGG	3 cut(s)	34, 140, 746
SduI	GDGCHC	1 cut(s)	222
SfaNI	GCATC	1 cut(s)	340
Sfr274I	CTCGAG	1 cut(s)	221
SlaI	CTCGAG	1 cut(s)	221
SmiMI	CAYNNNNRTG	1 cut(s)	149
SmlI	CTYRAG	2 cut(s)	221, 644
SmoI	CTYRAG	2 cut(s)	221, 644
Sse9I	AATT	5 cut(s)	130, 277, 329, 459, 551
SsiI	CCGC	2 cut(s)	303, 778
SspI	AATATT	1 cut(s)	700
SspMI	CTAG	2 cut(s)	206, 585
StyD4I	CCNGG	3 cut(s)	32, 138, 744
TaaI	ACNGT	1 cut(s)	739
TaiI	ACGT	1 cut(s)	419
TaqI	TCGA	5 cut(s)	66, 156, 222, 327, 864
TasI	AATT	5 cut(s)	130, 277, 329, 459, 551
TauI	GCSGC	1 cut(s)	781
TfiI	GAWTC	1 cut(s)	22
Tru1I	TTAA	4 cut(s)	9, 129, 645, 855
Tru9I	TTAA	4 cut(s)	9, 129, 645, 855
TscAI	CASTG	4 cut(s)	297, 538, 579, 742
TseFI	GTSAC	1 cut(s)	589
TseI	GCWGC	2 cut(s)	232, 581
Tsp45I	GTSAC	1 cut(s)	589
TspDTI	ATGAA	3 cut(s)	410, 679, 707
TspGWI	ACGGA	1 cut(s)	123
TspRI	CASTG	4 cut(s)	297, 538, 579, 742
Vha464I	CTTAAG	1 cut(s)	644
VspI	ATTAAT	1 cut(s)	129
XapI	RAATTY	2 cut(s)	277, 551
XhoI	CTCGAG	1 cut(s)	221
XspI	CTAG	2 cut(s)	206, 585
ZraI	GACGTC	1 cut(s)	417

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.