Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

31968162 .. 31973391

Gene Structure 5230 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002304

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3642 bp

ATGGGCCATTGCTGCATGAATCACACCGGGACTCCTCTCTCTTCCATTTTTGCTCAATCCAACCTCTCCCATCTCTCCACGCAACCAAACCCCTTCCGCTTCTTCCTCTACTCCAAGACCCACCGAAACGCCGCCGTTCCGTCTCTCAGACTCCGCTCATGGCCCACTTCGACGCCGCCGAGCCTCCGAGTAGCCACCTCGGTTCGGAGCGCCTCCAAGGAAGAACCAGCGCCGCCCAAGAAGCCGAATCAGCAGCAGCAGCTGGGGAAAAGGACCGACCTGAAGAAGATTCTGATACTGGGCGCCGGCCCCATTGTGATCGGGCAAGCCTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAGGCTTTGAAAGAAGAAGGATACGACGTCGTTTTGATCAATTCCAACCCGGCCACCATCATGACCGACCCCGATTTGGCCGACCGGACTTACATTACGCCGATGACGCCGGAGCTGGTGGAGCAAGTCCTCGAAAAGGAGCGCCCCGACGCTCTCCTCCCCACCATGGGAGGCCAGACGGCGTTGAACCTCGCCGTTGCGTTAGCCGAGAGCGGTGCGCTTGAAAAGTATGGGGTGGAGTTGATCGGAGCCAAGCTGGAGGCCATAAAGAAAGCAGAGGATAGGGATTTGTTCAAGCAGGCCATGAACAACATTGGGATCAAAACGCCGCCGTCTGGAATTGCCAACACTTATGAGGAATGCGTCGAAATCGCCAATGAGATTGGTGAGTTTCCCTTGATCATTAGGCCGGCTTTTACTCTAGGAGGGACGGGAGGTGGAATTGCTTATAATAAGGAGGAGTTTGAGACTATCTGTAAGTCGGGGCTGGCGGCGAGCTTGACTTCTCAGATTTTGGTGGAGAAGTCATTGTTGGGGTGGAAGGAGTATGAGCTTGAGGTCATGAGGGACTTGGCAGACAATGTCGTTATTATTTGCTCCATTGAGAATATTGACCCAATGGGGGTTCATACCGGGGACTCTATCACCGTGGCGCCTGCACAGACTTTGACTGATAAGGAGTATCAGCGCCTCAGAGACTATTCCATTGCTATAATAAGGGAGATTGGAGTGGAATGTGGTGGCTCTAATGTGCAGTTTGCGGTGAACCCCATAGATGGGGAGGTTATGGTGATTGAGATGAATCCTAGGGTGTCCCGGTCCTCGGCTCTCGCATCCAAAGCTACTGGATTTCCGATAGCGAAAATGGCTGCTAAGTTGTCAGTTGGGTATTCCTTGGATCAAATTCCTAATGACATTACAAAGAAGACACCGGCTAGTTTTGAACCTTCCATAGACTATGTGGTAACAAAGATTCCCAGGTTTGCTTTTGAGAAATTTCCTGGTTCACAGCCAATATTGACGACCCAGATGAAATCTGTTGGTGAGTCAATGGCTCTGGGTCGTACATTTCAAGAGTCCTTTCAGAAGGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGCAAAATTAAGGAACTGGACTGGGGTTGGGACAAGTTGAAATATAGCCTCCGAGTCCCTAACCCAGATCGAATCCATGCCGTATATGCTGCAATGAAGAAGGGGATGAAGGTAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTCAAGGAGTTAGTAGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGAGTTCTATGAAGTGAAGAAAAGAGGTTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCTTCTGAAAATGAAGTGCGGTTGAAGAGACTATTGTTAGGTGTTACTCCAGCATATAAGCGGGTAGATACGTGCGCTGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCTTCCTATGACTTCGAGTGTGAATCAGCTCCGACCCTAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGCATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTACAGAAAGCAGGATATGAAACGATTATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACAAGCGACCGTCTATATTTTGAGCCACTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTCGGAGGACAAACACCTCTGAAGCTGGCACTTCCCCTCCAGCAGTATTTAGACGAAAACAAGCCGAGATGTGCAAGTGGGATTGGACATGTACGCATTTGGGGTACAACACCTGACTCAATAGATGCTGCTGAGGACAGGGAGAAGTTCAATGCAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGAATTGCAAAGAGCGAAGCAGATGCTCTTGCAATTGCAACAGACATTGGCTACCCTGTTGTTGTCCGACCTTCCTATGTATTAGGTGGCCGTGCCATGGAGATAGTCTATAGTGATGAAAAACTTGTGACCTACCTTGAAACTGCTGTTGAGGTGGACCCAGAACGCCCTGTATTGATTGACAGGTATCTGTCAGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATTGAGCAGGCTGGGGTCCATTCTGGTGACTCTGCTTGCTCAATTCCGACAAAAACCATCCCAGCTTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCCAAGAGGCTGAATGTGTGCGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGTGATGTTTATTTACTTGAGGCAAATCCTCGTGCTTCCCGTACAGTCCCATTTGTGTCCAAGGCAATTGGACATCCATTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGACCTTAATTTCACAAAAGAAGTCATTCCTGCACATGTGTCGGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTACTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCCATTGCATTTGCCAAGGCACAGATTGCTGCTGGACAGAAGCCACCACTTTCTGGCACTGTGTTCTTAAGCTTAAATGACTTAACCAAACCTCATCTTGAAAGGATTGCAACTGCATTTTTAGGCCTTGGATTTAAGATTGTTTCAACTTCAGGGACAGCTCATTTTCTTGAATTAGCGAAAATTCCCGTGGAACGAGTGCTGAAGCTGCATGAGGGGCGACCTAATGCTGGTGACATGGTTGCAAATGGACAAATCCAGTTGATGGTGATCACAAGTTCTGGGGATGCACATGATCAAATTGATGGACGGCAGCTAAGGAGGATGGCTCTTGCTTACAAGATTCCAGTAATTACCACAGTTGCCGGAGCATTGGCCACTGCAGAAGCAATAAAGAGTCTAAAGTCCAGCTCCATTAAAATGATTGCTCTTCAGGACTTCTTTGATGATGAGAACAAAGCTGTCGATTGGAGCGGTCGCCGTCATTGCCCCTCCCTAGGGCAGTCCTTGGCCGAAGCTCCATTAGTTGCCTTGAAGGAGTTTCCATTGAACCCCAGTCGTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1214

Amino Acids

132.86

Weight (kDa)

5.7

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	94 - 211	1.1e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	215 - 422	1.3e-71	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	514 - 592	4.7e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	645 - 769	1.4e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	773 - 976	6.2e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	781 - 947	8.5e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	800 - 945	7.3e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1059 - 1144	3.8e-20	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	813
AatII	GACGTC	1 cut(s)	393
AccB1I	GGYRCC	2 cut(s)	302, 1015
AccB7I	CCANNNNNTGG	2 cut(s)	3128, 3340
AccBSI	CCGCTC	3 cut(s)	156, 576, 3549
AclWI	GGATC	3 cut(s)	689, 1269, 2177
AcoI	YGGCCR	6 cut(s)	414, 441, 2461, 2748, 3450, 3585
AcsI	RAATTY	6 cut(s)	1266, 1358, 1614, 1988, 2056, 3258
AcuI	CTGAAG	5 cut(s)	302, 2222, 3210, 3299, 3491
AcyI	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
AfaI	GTAC	6 cut(s)	1429, 1892, 2274, 2287, 2848, 3018
AflII	CTTAAG	1 cut(s)	3142
AflIII	ACRYGT	2 cut(s)	2269, 2962
AjnI	CCWGG	4 cut(s)	1340, 1363, 1689, 2901
AjuI	GAANNNNNNNTTGG	2 cut(s)	878, 910
Alw21I	GWGCWC	1 cut(s)	2643
Alw26I	GTCTC	5 cut(s)	147, 824, 1053, 1804, 2925
AlwI	GGATC	3 cut(s)	689, 1269, 2177
AlwNI	CAGNNNCTG	1 cut(s)	262
Ama87I	CYCGRG	1 cut(s)	346
ApoI	RAATTY	6 cut(s)	1266, 1358, 1614, 1988, 2056, 3258
Asp700I	GAANNNNTTC	2 cut(s)	2952, 2982
AspA2I	CCTAGG	2 cut(s)	1169, 3571
AspLEI	GCGC	8 cut(s)	212, 232, 305, 507, 583, 1018, 1053, 1859
AspS9I	GGNCC	9 cut(s)	4, 162, 273, 308, 1182, 1961, 2530, 2659, 3026
AsuC2I	CCSGG	4 cut(s)	28, 413, 997, 1180
AvaI	CYCGRG	1 cut(s)	346
AvaII	GGWCC	5 cut(s)	273, 1182, 1961, 2530, 2659
AvrII	CCTAGG	2 cut(s)	1169, 3571
BalI	TGGCCA	2 cut(s)	2750, 3452
BanI	GGYRCC	2 cut(s)	302, 1015
BauI	CACGAG	1 cut(s)	2835
BbsI	GAAGAC	1 cut(s)	1295
Bbv12I	GWGCWC	1 cut(s)	2643
BbvCI	CCTCAGC	1 cut(s)	2313
BceAI	ACGGC	8 cut(s)	119, 542, 558, 679, 1559, 2448, 3401, 3540
BcgI	CGANNNNNNTGC	6 cut(s)	560, 594, 2378, 2412, 2949, 2983
BciT130I	CCWGG	4 cut(s)	1342, 1365, 1691, 2903
BciVI	GTATCC	1 cut(s)	377
BclI	TGATCA	4 cut(s)	399, 762, 3345, 3370
BcnI	CCSGG	4 cut(s)	28, 413, 997, 1180
BcoDI	GTCTC	5 cut(s)	147, 824, 1053, 1804, 2925
BfaI	CTAG	5 cut(s)	785, 1170, 1299, 1695, 3572
BfmI	CTRYAG	4 cut(s)	1860, 2024, 2482, 3456
BfoI	RGCGCY	6 cut(s)	213, 233, 306, 508, 1019, 1054
BfrI	CTTAAG	1 cut(s)	3142
BfuI	GTATCC	1 cut(s)	377
BlnI	CCTAGG	2 cut(s)	1169, 3571
Bme1390I	CCNGG	8 cut(s)	28, 413, 997, 1180, 1342, 1365, 1691, 2903
Bme18I	GGWCC	5 cut(s)	273, 1182, 1961, 2530, 2659
BmeT110I	CYCGRG	1 cut(s)	346
BmgT120I	GGNCC	9 cut(s)	4, 162, 273, 308, 1182, 1961, 2530, 2659, 3026
BmiI	GGNNCC	7 cut(s)	304, 310, 613, 1017, 1646, 2532, 2660
BmrFI	CCNGG	8 cut(s)	28, 413, 997, 1180, 1342, 1365, 1691, 2903
BmrI	ACTGGG	3 cut(s)	308, 1525, 3624
BmsI	GCATC	7 cut(s)	1205, 2296, 2386, 2560, 2581, 3024, 3352
BmuI	ACTGGG	3 cut(s)	308, 1525, 3624
BpiI	GAAGAC	1 cut(s)	1295
BplI	GAGNNNNNCTC	2 cut(s)	3388, 3420
BpmI	CTGGAG	3 cut(s)	641, 1815, 2204
Bpu10I	CCTNAGC	2 cut(s)	2313, 3392
BpuEI	CTTGAG	4 cut(s)	339, 938, 1643, 2843
BpuMI	CCSGG	4 cut(s)	28, 413, 997, 1180
BsaAI	YACGTR	1 cut(s)	1854
BsaBI	GATNNNNATC	1 cut(s)	3344
BsaHI	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
BsaWI	WCCGGW	1 cut(s)	447
BsaXI	ACNNNNNCTCC	4 cut(s)	16, 46, 2202, 2232
Bse118I	RCCGGY	3 cut(s)	305, 772, 1294
Bse1I	ACTGG	8 cut(s)	303, 1213, 1515, 1520, 3049, 3334, 3422, 3630
Bse3DI	GCAATG	5 cut(s)	7, 1068, 1593, 3078, 3559
Bse8I	GATNNNNATC	1 cut(s)	3344
BseBI	CCWGG	4 cut(s)	1342, 1365, 1691, 2903
BseGI	GGATG	9 cut(s)	1196, 1490, 1605, 1726, 2012, 2700, 2878, 3367, 3405
BseJI	GATNNNNATC	1 cut(s)	3344
BseMI	GCAATG	5 cut(s)	7, 1068, 1593, 3078, 3559
BseMII	CTCAG	6 cut(s)	160, 884, 1069, 1616, 2304, 3021
BseNI	ACTGG	8 cut(s)	303, 1213, 1515, 1520, 3049, 3334, 3422, 3630
BseRI	GAGGAG	3 cut(s)	24, 509, 836
BseYI	CCCAGC	3 cut(s)	262, 2654, 2704
BsgI	GTGCAG	4 cut(s)	1005, 1136, 2198, 2943
Bsh1285I	CGRYCG	3 cut(s)	448, 2101, 3553
BshNI	GGYRCC	2 cut(s)	302, 1015
BsiEI	CGRYCG	3 cut(s)	448, 2101, 3553
BsiHKAI	GWGCWC	1 cut(s)	2643
BsiHKCI	CYCGRG	1 cut(s)	346
BsmAI	GTCTC	5 cut(s)	147, 824, 1053, 1804, 2925
BsmBI	CGTCTC	1 cut(s)	147
BsmI	GAATGC	1 cut(s)	728
BsoBI	CYCGRG	1 cut(s)	346
Bsp1286I	GDGCHC	1 cut(s)	2643
Bsp19I	CCATGG	2 cut(s)	528, 2469
BspCNI	CTCAG	6 cut(s)	159, 883, 1068, 1617, 2305, 3022
BspHI	TCATGA	4 cut(s)	423, 924, 1618, 2926
BspLI	GGNNCC	7 cut(s)	304, 310, 613, 1017, 1646, 2532, 2660
BspMAI	CTGCAG	2 cut(s)	1864, 3460
BspPI	GGATC	3 cut(s)	689, 1269, 2177
BspQI	GCTCTTC	1 cut(s)	3510
BspT107I	GGYRCC	2 cut(s)	302, 1015
BspTI	CTTAAG	1 cut(s)	3142
BsrBI	CCGCTC	3 cut(s)	156, 576, 3549
BsrDI	GCAATG	5 cut(s)	7, 1068, 1593, 3078, 3559
BsrFI	RCCGGY	3 cut(s)	305, 772, 1294
BsrI	ACTGG	8 cut(s)	303, 1213, 1515, 1520, 3049, 3334, 3422, 3630
BssAI	RCCGGY	3 cut(s)	305, 772, 1294
BssNI	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
BssSI	CACGAG	1 cut(s)	2835
Bst2BI	CACGAG	1 cut(s)	2835
Bst2UI	CCWGG	4 cut(s)	1342, 1365, 1691, 2903
Bst4CI	ACNGT	8 cut(s)	1012, 2074, 2102, 2125, 2783, 2851, 3136, 3436
Bst6I	CTCTTC	5 cut(s)	46, 1802, 1900, 2928, 3510
BstACI	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
BstAFI	CTTAAG	1 cut(s)	3142
BstAPI	GCANNNNNTGC	1 cut(s)	3101
BstBAI	YACGTR	1 cut(s)	1854
BstDEI	CTNAG	8 cut(s)	146, 870, 1055, 1236, 1625, 2313, 3030, 3392
BstDSI	CCRYGG	4 cut(s)	528, 1011, 2469, 3264
BstENI	CCTNNNNNAGG	1 cut(s)	497
BstF5I	GGATG	9 cut(s)	1196, 1490, 1605, 1726, 2012, 2700, 2878, 3367, 3405
BstH2I	RGCGCY	6 cut(s)	213, 233, 306, 508, 1019, 1054
BstHHI	GCGC	8 cut(s)	212, 232, 305, 507, 583, 1018, 1053, 1859
BstMAI	GTCTC	5 cut(s)	147, 824, 1053, 1804, 2925
BstMCI	CGRYCG	3 cut(s)	448, 2101, 3553
BstNI	CCWGG	4 cut(s)	1342, 1365, 1691, 2903
BstNSI	RCATGY	2 cut(s)	2273, 2966
BstSCI	CCNGG	8 cut(s)	26, 411, 995, 1178, 1340, 1363, 1689, 2901
BstSFI	CTRYAG	4 cut(s)	1860, 2024, 2482, 3456
BstV2I	GAAGAC	1 cut(s)	1295
BsuI	GTATCC	1 cut(s)	377
BtgI	CCRYGG	4 cut(s)	528, 1011, 2469, 3264
BtsCI	GGATG	9 cut(s)	1196, 1490, 1605, 1726, 2012, 2700, 2878, 3367, 3405
BtsI	GCAGTG	1 cut(s)	3453
BtsIMutI	CAGTG	5 cut(s)	1759, 2117, 3042, 3132, 3453
CaiI	CAGNNNCTG	1 cut(s)	262
CciI	TCATGA	4 cut(s)	423, 924, 1618, 2926
CfoI	GCGC	8 cut(s)	212, 232, 305, 507, 583, 1018, 1053, 1859
Cfr10I	RCCGGY	3 cut(s)	305, 772, 1294
Cfr13I	GGNCC	9 cut(s)	4, 162, 273, 308, 1182, 1961, 2530, 2659, 3026
CseI	GACGC	4 cut(s)	181, 478, 521, 715
Csp6I	GTAC	6 cut(s)	1428, 1891, 2273, 2286, 2847, 3017
CviQI	GTAC	6 cut(s)	1428, 1891, 2273, 2286, 2847, 3017
DdeI	CTNAG	8 cut(s)	146, 870, 1055, 1236, 1625, 2313, 3030, 3392
DinI	GGCGCC	2 cut(s)	304, 1017
EaeI	YGGCCR	6 cut(s)	414, 441, 2461, 2748, 3450, 3585
Eam1104I	CTCTTC	5 cut(s)	46, 1802, 1900, 2928, 3510
EarI	CTCTTC	5 cut(s)	46, 1802, 1900, 2928, 3510
Eco147I	AGGCCT	1 cut(s)	3201
Eco47I	GGWCC	5 cut(s)	273, 1182, 1961, 2530, 2659
Eco57I	CTGAAG	5 cut(s)	302, 2222, 3210, 3299, 3491
Eco88I	CYCGRG	1 cut(s)	346
EcoNI	CCTNNNNNAGG	1 cut(s)	497
EcoRI	GAATTC	1 cut(s)	2056
EcoRII	CCWGG	4 cut(s)	1340, 1363, 1689, 2901
EgeI	GGCGCC	2 cut(s)	304, 1017
EheI	GGCGCC	2 cut(s)	304, 1017
Esp3I	CGTCTC	1 cut(s)	147
FalI	AAGNNNNNCTT	2 cut(s)	2970, 3002
FauI	CCCGC	2 cut(s)	1836, 2762
FbaI	TGATCA	4 cut(s)	399, 762, 3345, 3370
FokI	GGATG	9 cut(s)	1183, 1497, 1612, 1733, 1999, 2687, 2865, 3374, 3412
FspBI	CTAG	5 cut(s)	785, 1170, 1299, 1695, 3572
GlaI	GCGC	8 cut(s)	211, 231, 304, 506, 582, 1017, 1052, 1858
GsaI	CCCAGC	3 cut(s)	266, 2658, 2708
GsuI	CTGGAG	3 cut(s)	641, 1815, 2204
HaeII	RGCGCY	6 cut(s)	213, 233, 306, 508, 1019, 1054
HgaI	GACGC	4 cut(s)	181, 478, 521, 715
HhaI	GCGC	8 cut(s)	212, 232, 305, 507, 583, 1018, 1053, 1859
Hin1I	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
Hin6I	GCGC	8 cut(s)	210, 230, 303, 505, 581, 1016, 1051, 1857
HinP1I	GCGC	8 cut(s)	210, 230, 303, 505, 581, 1016, 1051, 1857
HindIII	AAGCTT	2 cut(s)	357, 3145
Hpy166II	GTNNAC	3 cut(s)	1129, 1370, 2530
Hpy8I	GTNNAC	3 cut(s)	1129, 1370, 2530
Hpy99I	CGWCG	5 cut(s)	175, 392, 395, 515, 731
HpyCH4III	ACNGT	8 cut(s)	1012, 2074, 2102, 2125, 2783, 2851, 3136, 3436
HpyCH4IV	ACGT	2 cut(s)	390, 1853
HpyF3I	CTNAG	8 cut(s)	146, 870, 1055, 1236, 1625, 2313, 3030, 3392
HpySE526I	ACGT	2 cut(s)	390, 1853
Hsp92I	GRCGYC	5 cut(s)	173, 303, 390, 470, 1016
HspAI	GCGC	8 cut(s)	210, 230, 303, 505, 581, 1016, 1051, 1857
KasI	GGCGCC	2 cut(s)	302, 1015
KroI	GCCGGC	2 cut(s)	305, 772
KroNI	GCCGGC	2 cut(s)	307, 774
Ksp22I	TGATCA	4 cut(s)	399, 762, 3345, 3370
LguI	GCTCTTC	1 cut(s)	3510
LweI	GCATC	7 cut(s)	1205, 2296, 2386, 2560, 2581, 3024, 3352
MaeI	CTAG	5 cut(s)	785, 1170, 1299, 1695, 3572
MaeII	ACGT	2 cut(s)	390, 1853
MaeIII	GTNAC	6 cut(s)	1327, 1754, 1825, 2500, 2669, 3308
MbiI	CCGCTC	3 cut(s)	156, 576, 3549
MfeI	CAATTG	2 cut(s)	2406, 2871
MhlI	GDGCHC	1 cut(s)	2643
MlsI	TGGCCA	2 cut(s)	2750, 3452
MluNI	TGGCCA	2 cut(s)	2750, 3452
Mly113I	GGCGCC	2 cut(s)	303, 1016
MmeI	TCCRAC	5 cut(s)	84, 432, 1949, 2464, 2714
Mox20I	TGGCCA	2 cut(s)	2750, 3452
MroNI	GCCGGC	2 cut(s)	305, 772
MroXI	GAANNNNTTC	2 cut(s)	2952, 2982
MscI	TGGCCA	2 cut(s)	2750, 3452
MseI	TTAA	9 cut(s)	1503, 2343, 2351, 2934, 3143, 3149, 3158, 3210, 3492
MslI	CAYNNNNRTG	4 cut(s)	422, 2613, 2667, 3494
Msp20I	TGGCCA	2 cut(s)	2750, 3452
MspA1I	CMGCKG	1 cut(s)	262
MspCI	CTTAAG	1 cut(s)	3142
MspR9I	CCNGG	8 cut(s)	28, 413, 997, 1180, 1342, 1365, 1691, 2903
MunI	CAATTG	2 cut(s)	2406, 2871
Mva1269I	GAATGC	1 cut(s)	728
MvaI	CCWGG	4 cut(s)	1342, 1365, 1691, 2903
NaeI	GCCGGC	2 cut(s)	307, 774
NarI	GGCGCC	2 cut(s)	303, 1016
NciI	CCSGG	4 cut(s)	28, 413, 997, 1180
NcoI	CCATGG	2 cut(s)	528, 2469
NgoMIV	GCCGGC	2 cut(s)	305, 772
NlaIV	GGNNCC	7 cut(s)	304, 310, 613, 1017, 1646, 2532, 2660
NmeAIII	GCCGAG	4 cut(s)	204, 595, 1166, 2271
NmuCI	GTSAC	4 cut(s)	1754, 2500, 2669, 3308
NspI	RCATGY	2 cut(s)	2273, 2966
PagI	TCATGA	4 cut(s)	423, 924, 1618, 2926
PceI	AGGCCT	1 cut(s)	3201
PciI	ACATGT	2 cut(s)	2269, 2962
PciSI	GCTCTTC	1 cut(s)	3510
PcsI	WCGNNNNNNNCGW	2 cut(s)	176, 2842
PctI	GAATGC	1 cut(s)	728
PdiI	GCCGGC	2 cut(s)	307, 774
PdmI	GAANNNNTTC	2 cut(s)	2952, 2982
PfeI	GAWTC	8 cut(s)	19, 247, 289, 1165, 1336, 1566, 1916, 3418
PflFI	GACNNNGTC	1 cut(s)	944
PflMI	CCANNNNNTGG	2 cut(s)	3128, 3340
PluTI	GGCGCC	2 cut(s)	306, 1019
Ppu21I	YACGTR	1 cut(s)	1854
PscI	ACATGT	2 cut(s)	2269, 2962
PsiI	TTATAA	1 cut(s)	813
Psp6I	CCWGG	4 cut(s)	1340, 1363, 1689, 2901
PspFI	CCCAGC	3 cut(s)	262, 2654, 2704
PspGI	CCWGG	4 cut(s)	1340, 1363, 1689, 2901
PspN4I	GGNNCC	7 cut(s)	304, 310, 613, 1017, 1646, 2532, 2660
PspPI	GGNCC	9 cut(s)	4, 162, 273, 308, 1182, 1961, 2530, 2659, 3026
PstI	CTGCAG	2 cut(s)	1864, 3460
PstNI	CAGNNNCTG	1 cut(s)	262
PsyI	GACNNNGTC	1 cut(s)	944
PvuII	CAGCTG	1 cut(s)	262
RsaI	GTAC	6 cut(s)	1429, 1892, 2274, 2287, 2848, 3018
RsaNI	GTAC	6 cut(s)	1428, 1891, 2273, 2286, 2847, 3017
RseI	CAYNNNNRTG	4 cut(s)	422, 2613, 2667, 3494
SapI	GCTCTTC	1 cut(s)	3510
SaqAI	TTAA	9 cut(s)	1503, 2343, 2351, 2934, 3143, 3149, 3158, 3210, 3492
Sau96I	GGNCC	9 cut(s)	4, 162, 273, 308, 1182, 1961, 2530, 2659, 3026
ScrFI	CCNGG	8 cut(s)	28, 413, 997, 1180, 1342, 1365, 1691, 2903
SduI	GDGCHC	1 cut(s)	2643
SfaNI	GCATC	7 cut(s)	1205, 2296, 2386, 2560, 2581, 3024, 3352
SfcI	CTRYAG	4 cut(s)	1860, 2024, 2482, 3456
SfoI	GGCGCC	2 cut(s)	304, 1017
SinI	GGWCC	5 cut(s)	273, 1182, 1961, 2530, 2659
SmiMI	CAYNNNNRTG	4 cut(s)	422, 2613, 2667, 3494
SmlI	CTYRAG	5 cut(s)	354, 917, 1658, 2822, 3142
SmoI	CTYRAG	5 cut(s)	354, 917, 1658, 2822, 3142
SseBI	AGGCCT	1 cut(s)	3201
SspDI	GGCGCC	2 cut(s)	302, 1015
SspI	AATATT	2 cut(s)	973, 1380
SspMI	CTAG	5 cut(s)	785, 1170, 1299, 1695, 3572
StuI	AGGCCT	1 cut(s)	3201
StyD4I	CCNGG	8 cut(s)	26, 411, 995, 1178, 1340, 1363, 1689, 2901
TaaI	ACNGT	8 cut(s)	1012, 2074, 2102, 2125, 2783, 2851, 3136, 3436
TaiI	ACGT	2 cut(s)	393, 1856
TaqI	TCGA	9 cut(s)	170, 339, 495, 729, 1459, 1564, 1908, 1969, 3540
TaqII	GACCGA	2 cut(s)	290, 443
TatI	WGTACW	2 cut(s)	1890, 3016
TauI	GCSGC	5 cut(s)	134, 178, 235, 694, 857
TfiI	GAWTC	8 cut(s)	19, 247, 289, 1165, 1336, 1566, 1916, 3418
Tru1I	TTAA	9 cut(s)	1503, 2343, 2351, 2934, 3143, 3149, 3158, 3210, 3492
Tru9I	TTAA	9 cut(s)	1503, 2343, 2351, 2934, 3143, 3149, 3158, 3210, 3492
TscAI	CASTG	5 cut(s)	1759, 2124, 3049, 3139, 3460
TseFI	GTSAC	4 cut(s)	1754, 2500, 2669, 3308
Tsp45I	GTSAC	4 cut(s)	1754, 2500, 2669, 3308
TspGWI	ACGGA	1 cut(s)	129
TspRI	CASTG	5 cut(s)	1759, 2124, 3049, 3139, 3460
Tth111I	GACNNNGTC	1 cut(s)	944
Van91I	CCANNNNNTGG	2 cut(s)	3128, 3340
Vha464I	CTTAAG	1 cut(s)	3142
VpaK11BI	GGWCC	5 cut(s)	273, 1182, 1961, 2530, 2659
XagI	CCTNNNNNAGG	1 cut(s)	497
XapI	RAATTY	6 cut(s)	1266, 1358, 1614, 1988, 2056, 3258
XceI	RCATGY	2 cut(s)	2273, 2966
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1321
XmaJI	CCTAGG	2 cut(s)	1169, 3571
XmnI	GAANNNNTTC	2 cut(s)	2952, 2982
XspI	CTAG	5 cut(s)	785, 1170, 1299, 1695, 3572
ZraI	GACGTC	1 cut(s)	391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.