Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Reverse (-)

35679694 .. 35684836

Gene Structure 5143 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G027510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3573 bp

ATGGGCCATTGCTGCATGAATCACACCGACACTGCTCTCTCTTCCATTTTTGCTCAATCCAACCTCTCCTATCTCTCCACGCAACCAAACCCCTTCCGCTTCTTCCTCCGCTCCAAGACCCACCGAAACGCCGCCGTTCCCCCTCTCAGACTCCGCTCATGGCCCACTTCGACGCAGCCGAGCCTCCGAGCAGCCACCTCGGTTCGGAGCGCCTCCAAAGAAGAACCAGCGCCGCCCAAGAAGCAGCAGCGGCAGCTGGGGAAAAGGACCGACCTGAAGAAGATTCTGATACTGGGCGCCGGCCCCATTGTGATTGGGCAAGCCTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAGGCTTTGAAGGAAGAAGGATACGACGTCGTTTTGATCAATTCCAACCCGGCCACCATCATGACCGACCCCGATTTGGCCGACCGGACTTACATTACGCCGATGACGCCGGAGCTGGTGGAGCAAGTCCTCGAAAAGGAGCGCCCCGACGCTCTCCTCCCCACCATGGGAGGCCAGACGGCGTTGAACCTCGCCGTTGCGTTAGCCGAGAGCGGTGCGCTTGAAAAGTATGGGGTGGAGCTGATCGGAGCCAAGCTGGAGGCCATAAAGAAAGCAGAGGATAGGGATTTGTTCAAGCAGGCCATGAACAACATTGGGATCAAAACGCCGCCGTCTGGAATTGCCAACACTTATGAGGAATGCGTCGAAATCGCCAATGAGATTGGTGAGTTTCCCTTGATCATTAGGCCGGCTTTTACTCTAGGAGGGACAGGGGGTGGAATTGCTTATAATAAGGAGGAGTTTGAGACCATCTGTAAGTCGGGGCTGGCGGCGAGCTTGACTTCTCAGATTTTGGTGGAGAAGTCATTGTTGGGGTGGAAGGAGTATGAGCTTGAGGTCATGAGGGACTTGGCAGACAATGTGGTTATTATTTGCTCCATTGAGAATATTGACCCAATGGGGGTTCATACTGGGGATTCTATCACCGTGGCGCCTGCGCAGACTTTGACTGATAAGGAGTATCAGCGGCTAAGAGACTATTCCATTGCTATAATAAGGGAGATTGGAGTGGAATGTGGTGGCTCTAATGTGCAGTTTGCGGTGAATCCCAAAGATGGGGAGGTTATGGTGATTGAGATGAATCCTAGGGTGTCGCGGTCCTCGGCTCTCGCATCCAAAGCTACTGGATTTCCGATAGCGAAAATGGCTGCCAAGTTGTCAGTTGGGTATTCATTGGATCAAATTCCCAATGACATTACAAAGAAGACACCGGCTAGTTTTGAACCTTCCATAGACTATGTGGTAACAAAGATTCCCAGGTTTGCTTTTGAGAAATTTCCTGGTTCACAGCCAATATTGACGACCCAGATGAAATCTGTTGGTGAGTCAATGGCTCTGGGTCGTACATTTCAAGAGTCCTTTCAGAAGGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGCAAAATTAAGGAACTGGACTGGGGTTGGGACAAGTTGAAATATAGCCTCCGAGTCCCTAACCCAGATCGAATCCATGCCGTATATGCTGCAATGAAGAAGGGGATGAAGGTAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTCAAGGAGTTAGTAGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGAGTTCTACGAAGTGAAGAAAAGAGGCTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCTTCTGAAAATGAAGTGCGGTTGAAGAGACTATTGTTAGGTGTTACTCCAGCATATAAGCGGGTAGATACGTGCGCTGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCTTCCTATGACTTTGAGTGTGAATCAGTTCCGACCCAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAAACAATTATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACGAGCGACCGTCTATATTTTGAGCCACTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTCGGAGGACAAACACCTCTGAAGCTGGCACTTCCCCTCCAGCAGTATTTAGACGAAAACAAGCCGAGATGTGCAAGTGGGATTGGACACGTGCGCATTTGGGGTACAACACCTGACTCCATAGATGCTGCTGAGGACAGGGAGAAGTTTAATGCAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGAATTGCAAAGAGCGAAGCAGATGCTCTTGCAATTGCAACAGACATTGGCTACCCTGTTGTTGTCCGACCTTCCTATGTATTAGGCGGCCGTGCCATGGAGATAGTCTATAGTGATGAAAAACTTGTGACCTACCTTGAAACTGCTGTTGAGGTGGACCCAGAACGCCCTGTATTGATAGACAGGTATCTGTCAGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTTGTCATTGGTGGGATAATGGAGCACATTGAGCAGGCTGGGGTCCATTCTGGTGACTCTGCTTGCTCAATTCCGACAAAAACCATCCCAGCTTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCCAAGAGGCTGAATGTGTGCGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGTGATGTTTATTTACTTGAGGCAAATCCTCGTGCTTCCCGTACAGTCCCATTTGTGTCCAAGGCAATTGGACATCCATTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGACCTTAATTTCACAAAAGAAGTCATTCCTGCACATGTGTCAGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTACTGCTTGGGCCCGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCCATTGCGTTTGCCAAGGCGCAGATTGCTGCTGGACAGAAGCCACCACTTTCTGGCACTGTGTTCTTGAGCTTAAATGACTTAACCAAACCTCATCTTGAAAGGATTGCAACCACATTTTTAGGCCTTGGATTTAAGATTGTCTCAACTTCAGGGACAGCTCATTTTCTAGAATTAGCGAAAATTCCCGTGGAACGAGTGCTGAAGCTGCATGAGGGGCGACCTAATGCTGGTGACATGGTTGCAAATGGACAAATCCAGTTGATGGTGATCACAAGTTCTGGGGATGCACATGATCAAATTGATGGACGGCAGCTAAGGAGGATGGCTCTTTCTTACAAGATTCCAGTAATTACCACAGTTGCCGGAGCATTGGCCACTGCAGAAGCAATAAAGAGTCTAAAGTCCAGCTCCATTAAAATGATTGCTCTTCAGGACTTCTTTGATGACGAGAACAAAGCTCTCGGTAATAAGAAGTTGCAGTCAGCCTCGTCCTCTATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1190

Amino Acids

130.42

Weight (kDa)

5.73

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	92 - 209	1e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	213 - 420	4.4e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	512 - 590	4.6e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	642 - 767	8e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	771 - 974	6.1e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	779 - 945	8.3e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	798 - 943	7.1e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1057 - 1142	7.1e-20	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	807
AatII	GACGTC	1 cut(s)	387
Acc16I	TGCGCA	2 cut(s)	1017, 2270
AccB1I	GGYRCC	2 cut(s)	296, 1009
AccB7I	CCANNNNNTGG	2 cut(s)	3122, 3334
AccBSI	CCGCTC	3 cut(s)	111, 156, 570
AccII	CGCG	1 cut(s)	1174
AclWI	GGATC	3 cut(s)	683, 1263, 2171
AcoI	YGGCCR	5 cut(s)	408, 435, 2455, 2742, 3444
AcsI	RAATTY	6 cut(s)	1260, 1352, 1608, 1982, 2050, 3252
AcuI	CTGAAG	7 cut(s)	296, 1729, 2003, 2216, 3204, 3293, 3485
AcvI	CACGTG	1 cut(s)	2266
AcyI	GRCGYC	4 cut(s)	297, 384, 464, 1010
AfaI	GTAC	5 cut(s)	1423, 1886, 2281, 2842, 3012
AflIII	ACRYGT	2 cut(s)	2263, 2956
AjnI	CCWGG	4 cut(s)	1334, 1357, 1683, 2895
AjuI	GAANNNNNNNTTGG	2 cut(s)	872, 904
Alw21I	GWGCWC	1 cut(s)	2637
Alw26I	GTCTC	5 cut(s)	818, 1047, 1798, 2919, 3217
AlwI	GGATC	3 cut(s)	683, 1263, 2171
Ama87I	CYCGRG	2 cut(s)	340, 3023
ApaI	GGGCCC	1 cut(s)	3024
ApoI	RAATTY	6 cut(s)	1260, 1352, 1608, 1982, 2050, 3252
Asp700I	GAANNNNTTC	3 cut(s)	1914, 2946, 2976
AspA2I	CCTAGG	1 cut(s)	1163
AsuC2I	CCSGG	1 cut(s)	407
AvaI	CYCGRG	2 cut(s)	340, 3023
AvaII	GGWCC	5 cut(s)	267, 1176, 1955, 2524, 2653
AvrII	CCTAGG	1 cut(s)	1163
BaeGI	GKGCMC	1 cut(s)	3024
BalI	TGGCCA	2 cut(s)	2744, 3446
BanI	GGYRCC	2 cut(s)	296, 1009
BanII	GRGCYC	1 cut(s)	3024
BauI	CACGAG	2 cut(s)	2086, 2829
BbrPI	CACGTG	1 cut(s)	2266
BbsI	GAAGAC	1 cut(s)	1289
Bbv12I	GWGCWC	1 cut(s)	2637
BbvCI	CCTCAGC	1 cut(s)	2307
BceAI	ACGGC	7 cut(s)	119, 536, 552, 673, 1553, 2442, 3395
BcgI	CGANNNNNNTGC	6 cut(s)	180, 214, 554, 588, 2372, 2406
BciT130I	CCWGG	4 cut(s)	1336, 1359, 1685, 2897
BciVI	GTATCC	1 cut(s)	371
BclI	TGATCA	4 cut(s)	393, 756, 3339, 3364
BcnI	CCSGG	1 cut(s)	407
BcoDI	GTCTC	5 cut(s)	818, 1047, 1798, 2919, 3217
BfaI	CTAG	5 cut(s)	779, 1164, 1293, 1689, 3239
BfmI	CTRYAG	3 cut(s)	1854, 2476, 3450
BfoI	RGCGCY	5 cut(s)	213, 233, 300, 502, 1013
BfuI	GTATCC	1 cut(s)	371
BlnI	CCTAGG	1 cut(s)	1163
Bme1390I	CCNGG	5 cut(s)	407, 1336, 1359, 1685, 2897
Bme18I	GGWCC	5 cut(s)	267, 1176, 1955, 2524, 2653
BmeT110I	CYCGRG	2 cut(s)	340, 3023
BmiI	GGNNCC	8 cut(s)	298, 304, 607, 1011, 1640, 2526, 2654, 3022
BmrFI	CCNGG	5 cut(s)	407, 1336, 1359, 1685, 2897
BmrI	ACTGGG	3 cut(s)	302, 999, 1519
BmsI	GCATC	7 cut(s)	1199, 2290, 2380, 2554, 2575, 3018, 3346
BmuI	ACTGGG	3 cut(s)	302, 999, 1519
BpiI	GAAGAC	1 cut(s)	1289
BplI	GAGNNNNNCTC	2 cut(s)	3382, 3414
BpmI	CTGGAG	3 cut(s)	635, 1809, 2198
Bpu10I	CCTNAGC	2 cut(s)	2307, 3386
BpuEI	CTTGAG	5 cut(s)	333, 932, 1637, 2837, 3157
BpuMI	CCSGG	1 cut(s)	407
BsaAI	YACGTR	2 cut(s)	1848, 2266
BsaBI	GATNNNNATC	1 cut(s)	3338
BsaHI	GRCGYC	4 cut(s)	297, 384, 464, 1010
BsaI	GGTCTC	1 cut(s)	818
BsaWI	WCCGGW	1 cut(s)	441
BsaXI	ACNNNNNCTCC	2 cut(s)	2196, 2226
Bse118I	RCCGGY	3 cut(s)	299, 766, 1288
Bse1I	ACTGG	8 cut(s)	297, 994, 1207, 1509, 1514, 3043, 3328, 3416
Bse3DI	GCAATG	4 cut(s)	7, 1062, 1587, 3072
Bse8I	GATNNNNATC	1 cut(s)	3338
BseBI	CCWGG	4 cut(s)	1336, 1359, 1685, 2897
BseGI	GGATG	9 cut(s)	1190, 1484, 1599, 1720, 2006, 2694, 2872, 3361, 3399
BseJI	GATNNNNATC	1 cut(s)	3338
BseMI	GCAATG	4 cut(s)	7, 1062, 1587, 3072
BseMII	CTCAG	4 cut(s)	160, 878, 1610, 2298
BseNI	ACTGG	8 cut(s)	297, 994, 1207, 1509, 1514, 3043, 3328, 3416
BseRI	GAGGAG	2 cut(s)	503, 830
BseSI	GKGCMC	1 cut(s)	3024
BseX3I	CGGCCG	1 cut(s)	2455
BseYI	CCCAGC	3 cut(s)	256, 2648, 2698
BsgI	GTGCAG	3 cut(s)	1130, 2192, 2937
Bsh1236I	CGCG	1 cut(s)	1174
Bsh1285I	CGRYCG	3 cut(s)	442, 2095, 2458
BshNI	GGYRCC	2 cut(s)	296, 1009
BsiEI	CGRYCG	3 cut(s)	442, 2095, 2458
BsiHKAI	GWGCWC	1 cut(s)	2637
BsiHKCI	CYCGRG	2 cut(s)	340, 3023
BsiSI	CCGG	7 cut(s)	300, 407, 442, 467, 767, 1289, 3435
BslFI	GGGAC	7 cut(s)	358, 799, 938, 1529, 1532, 2831, 3238
BsmAI	GTCTC	5 cut(s)	818, 1047, 1798, 2919, 3217
BsmFI	GGGAC	7 cut(s)	358, 799, 938, 1529, 1532, 2831, 3238
BsmI	GAATGC	1 cut(s)	722
Bso31I	GGTCTC	1 cut(s)	818
BsoBI	CYCGRG	2 cut(s)	340, 3023
Bsp120I	GGGCCC	1 cut(s)	3020
Bsp1286I	GDGCHC	2 cut(s)	2637, 3024
Bsp19I	CCATGG	2 cut(s)	522, 2463
BspCNI	CTCAG	4 cut(s)	159, 877, 1611, 2299
BspFNI	CGCG	1 cut(s)	1174
BspHI	TCATGA	4 cut(s)	417, 918, 1612, 2920
BspLI	GGNNCC	8 cut(s)	298, 304, 607, 1011, 1640, 2526, 2654, 3022
BspMAI	CTGCAG	2 cut(s)	1858, 3454
BspPI	GGATC	3 cut(s)	683, 1263, 2171
BspQI	GCTCTTC	1 cut(s)	3504
BspT107I	GGYRCC	2 cut(s)	296, 1009
BspTNI	GGTCTC	1 cut(s)	818
BsrBI	CCGCTC	3 cut(s)	111, 156, 570
BsrDI	GCAATG	4 cut(s)	7, 1062, 1587, 3072
BsrFI	RCCGGY	3 cut(s)	299, 766, 1288
BsrI	ACTGG	8 cut(s)	297, 994, 1207, 1509, 1514, 3043, 3328, 3416
BssAI	RCCGGY	3 cut(s)	299, 766, 1288
BssNI	GRCGYC	4 cut(s)	297, 384, 464, 1010
BssSI	CACGAG	2 cut(s)	2086, 2829
BssT1I	CCWWGG	7 cut(s)	522, 1163, 2463, 2859, 2998, 3084, 3196
Bst2BI	CACGAG	2 cut(s)	2086, 2829
Bst2UI	CCWGG	4 cut(s)	1336, 1359, 1685, 2897
Bst4CI	ACNGT	8 cut(s)	1006, 2068, 2096, 2119, 2777, 2845, 3130, 3430
Bst6I	CTCTTC	5 cut(s)	46, 1796, 1894, 2922, 3504
BstACI	GRCGYC	4 cut(s)	297, 384, 464, 1010
BstBAI	YACGTR	2 cut(s)	1848, 2266
BstDEI	CTNAG	6 cut(s)	146, 864, 1049, 1619, 2307, 3386
BstDSI	CCRYGG	4 cut(s)	522, 1005, 2463, 3258
BstENI	CCTNNNNNAGG	1 cut(s)	491
BstF5I	GGATG	9 cut(s)	1190, 1484, 1599, 1720, 2006, 2694, 2872, 3361, 3399
BstFNI	CGCG	1 cut(s)	1174
BstH2I	RGCGCY	5 cut(s)	213, 233, 300, 502, 1013
BstMAI	GTCTC	5 cut(s)	818, 1047, 1798, 2919, 3217
BstMCI	CGRYCG	3 cut(s)	442, 2095, 2458
BstNI	CCWGG	4 cut(s)	1336, 1359, 1685, 2897
BstNSI	RCATGY	1 cut(s)	2960
BstSCI	CCNGG	5 cut(s)	405, 1334, 1357, 1683, 2895
BstSFI	CTRYAG	3 cut(s)	1854, 2476, 3450
BstSLI	GKGCMC	1 cut(s)	3024
BstUI	CGCG	1 cut(s)	1174
BstV2I	GAAGAC	1 cut(s)	1289
BstZI	CGGCCG	1 cut(s)	2455
BsuI	GTATCC	1 cut(s)	371
BtgI	CCRYGG	4 cut(s)	522, 1005, 2463, 3258
BtsCI	GGATG	9 cut(s)	1190, 1484, 1599, 1720, 2006, 2694, 2872, 3361, 3399
BtsI	GCAGTG	2 cut(s)	30, 3447
BtsIMutI	CAGTG	6 cut(s)	30, 1753, 2111, 3036, 3126, 3447
CciI	TCATGA	4 cut(s)	417, 918, 1612, 2920
Cfr10I	RCCGGY	3 cut(s)	299, 766, 1288
CseI	GACGC	4 cut(s)	181, 472, 515, 709
Csp6I	GTAC	5 cut(s)	1422, 1885, 2280, 2841, 3011
CviQI	GTAC	5 cut(s)	1422, 1885, 2280, 2841, 3011
DdeI	CTNAG	6 cut(s)	146, 864, 1049, 1619, 2307, 3386
DinI	GGCGCC	2 cut(s)	298, 1011
EaeI	YGGCCR	5 cut(s)	408, 435, 2455, 2742, 3444
EagI	CGGCCG	1 cut(s)	2455
Eam1104I	CTCTTC	5 cut(s)	46, 1796, 1894, 2922, 3504
EarI	CTCTTC	5 cut(s)	46, 1796, 1894, 2922, 3504
EclXI	CGGCCG	1 cut(s)	2455
Eco130I	CCWWGG	7 cut(s)	522, 1163, 2463, 2859, 2998, 3084, 3196
Eco147I	AGGCCT	1 cut(s)	3195
Eco24I	GRGCYC	1 cut(s)	3024
Eco31I	GGTCTC	1 cut(s)	818
Eco47I	GGWCC	5 cut(s)	267, 1176, 1955, 2524, 2653
Eco52I	CGGCCG	1 cut(s)	2455
Eco57I	CTGAAG	7 cut(s)	296, 1729, 2003, 2216, 3204, 3293, 3485
Eco72I	CACGTG	1 cut(s)	2266
Eco88I	CYCGRG	2 cut(s)	340, 3023
EcoNI	CCTNNNNNAGG	1 cut(s)	491
EcoRI	GAATTC	1 cut(s)	2050
EcoRII	CCWGG	4 cut(s)	1334, 1357, 1683, 2895
EcoT14I	CCWWGG	7 cut(s)	522, 1163, 2463, 2859, 2998, 3084, 3196
EcoT38I	GRGCYC	1 cut(s)	3024
EgeI	GGCGCC	2 cut(s)	298, 1011
EheI	GGCGCC	2 cut(s)	298, 1011
ErhI	CCWWGG	7 cut(s)	522, 1163, 2463, 2859, 2998, 3084, 3196
FalI	AAGNNNNNCTT	2 cut(s)	2964, 2996
FaqI	GGGAC	7 cut(s)	358, 799, 938, 1529, 1532, 2831, 3238
FauI	CCCGC	2 cut(s)	1830, 2756
FbaI	TGATCA	4 cut(s)	393, 756, 3339, 3364
FokI	GGATG	9 cut(s)	1177, 1491, 1606, 1727, 1993, 2681, 2859, 3368, 3406
FriOI	GRGCYC	1 cut(s)	3024
FspAI	RTGCGCAY	1 cut(s)	2270
FspBI	CTAG	5 cut(s)	779, 1164, 1293, 1689, 3239
FspI	TGCGCA	2 cut(s)	1017, 2270
GsaI	CCCAGC	3 cut(s)	260, 2652, 2702
GsuI	CTGGAG	3 cut(s)	635, 1809, 2198
HaeII	RGCGCY	5 cut(s)	213, 233, 300, 502, 1013
HapII	CCGG	7 cut(s)	300, 407, 442, 467, 767, 1289, 3435
HgaI	GACGC	4 cut(s)	181, 472, 515, 709
Hin1I	GRCGYC	4 cut(s)	297, 384, 464, 1010
HindIII	AAGCTT	1 cut(s)	351
HpaII	CCGG	7 cut(s)	300, 407, 442, 467, 767, 1289, 3435
Hpy166II	GTNNAC	2 cut(s)	1364, 2524
Hpy8I	GTNNAC	2 cut(s)	1364, 2524
Hpy99I	CGWCG	5 cut(s)	175, 386, 389, 509, 725
HpyCH4III	ACNGT	8 cut(s)	1006, 2068, 2096, 2119, 2777, 2845, 3130, 3430
HpyCH4IV	ACGT	3 cut(s)	384, 1847, 2265
HpyF3I	CTNAG	6 cut(s)	146, 864, 1049, 1619, 2307, 3386
HpySE526I	ACGT	3 cut(s)	384, 1847, 2265
Hsp92I	GRCGYC	4 cut(s)	297, 384, 464, 1010
KasI	GGCGCC	2 cut(s)	296, 1009
KroI	GCCGGC	2 cut(s)	299, 766
KroNI	GCCGGC	2 cut(s)	301, 768
Ksp22I	TGATCA	4 cut(s)	393, 756, 3339, 3364
LguI	GCTCTTC	1 cut(s)	3504
LweI	GCATC	7 cut(s)	1199, 2290, 2380, 2554, 2575, 3018, 3346
MaeI	CTAG	5 cut(s)	779, 1164, 1293, 1689, 3239
MaeII	ACGT	3 cut(s)	384, 1847, 2265
MaeIII	GTNAC	6 cut(s)	1321, 1748, 1819, 2494, 2663, 3302
MbiI	CCGCTC	3 cut(s)	111, 156, 570
MfeI	CAATTG	2 cut(s)	2400, 2865
MhlI	GDGCHC	2 cut(s)	2637, 3024
MlsI	TGGCCA	2 cut(s)	2744, 3446
MluNI	TGGCCA	2 cut(s)	2744, 3446
Mly113I	GGCGCC	2 cut(s)	297, 1010
MlyI	GAGTC	9 cut(s)	144, 340, 1412, 1442, 1551, 2285, 2591, 2660, 3475
MmeI	TCCRAC	5 cut(s)	84, 426, 1943, 2458, 2708
Mox20I	TGGCCA	2 cut(s)	2744, 3446
MroNI	GCCGGC	2 cut(s)	299, 766
MroXI	GAANNNNTTC	3 cut(s)	1914, 2946, 2976
MscI	TGGCCA	2 cut(s)	2744, 3446
MseI	TTAA	9 cut(s)	1497, 2325, 2337, 2345, 2928, 3143, 3152, 3204, 3486
MslI	CAYNNNNRTG	4 cut(s)	416, 2607, 2661, 3488
Msp20I	TGGCCA	2 cut(s)	2744, 3446
MspA1I	CMGCKG	3 cut(s)	250, 256, 1045
MspI	CCGG	7 cut(s)	300, 407, 442, 467, 767, 1289, 3435
MspR9I	CCNGG	5 cut(s)	407, 1336, 1359, 1685, 2897
MunI	CAATTG	2 cut(s)	2400, 2865
Mva1269I	GAATGC	1 cut(s)	722
MvaI	CCWGG	4 cut(s)	1336, 1359, 1685, 2897
MvnI	CGCG	1 cut(s)	1174
NaeI	GCCGGC	2 cut(s)	301, 768
NarI	GGCGCC	2 cut(s)	297, 1010
NciI	CCSGG	1 cut(s)	407
NcoI	CCATGG	2 cut(s)	522, 2463
NgoMIV	GCCGGC	2 cut(s)	299, 766
NlaIV	GGNNCC	8 cut(s)	298, 304, 607, 1011, 1640, 2526, 2654, 3022
NmeAIII	GCCGAG	4 cut(s)	204, 589, 1160, 2265
NmuCI	GTSAC	4 cut(s)	1748, 2494, 2663, 3302
NsbI	TGCGCA	2 cut(s)	1017, 2270
NspI	RCATGY	1 cut(s)	2960
PagI	TCATGA	4 cut(s)	417, 918, 1612, 2920
PceI	AGGCCT	1 cut(s)	3195
PciI	ACATGT	1 cut(s)	2956
PciSI	GCTCTTC	1 cut(s)	3504
PcsI	WCGNNNNNNNCGW	2 cut(s)	176, 2836
PctI	GAATGC	1 cut(s)	722
PdiI	GCCGGC	2 cut(s)	301, 768
PdmI	GAANNNNTTC	3 cut(s)	1914, 2946, 2976
PfeI	GAWTC	9 cut(s)	19, 283, 995, 1123, 1159, 1330, 1560, 1910, 3412
PflMI	CCANNNNNTGG	2 cut(s)	3122, 3334
PleI	GAGTC	9 cut(s)	144, 340, 1411, 1441, 1550, 2285, 2591, 2660, 3474
PluTI	GGCGCC	2 cut(s)	300, 1013
PmaCI	CACGTG	1 cut(s)	2266
PmlI	CACGTG	1 cut(s)	2266
PpsI	GAGTC	9 cut(s)	144, 340, 1411, 1441, 1550, 2285, 2591, 2660, 3474
Ppu21I	YACGTR	2 cut(s)	1848, 2266
PscI	ACATGT	1 cut(s)	2956
PsiI	TTATAA	1 cut(s)	807
Psp6I	CCWGG	4 cut(s)	1334, 1357, 1683, 2895
PspCI	CACGTG	1 cut(s)	2266
PspFI	CCCAGC	3 cut(s)	256, 2648, 2698
PspGI	CCWGG	4 cut(s)	1334, 1357, 1683, 2895
PspN4I	GGNNCC	8 cut(s)	298, 304, 607, 1011, 1640, 2526, 2654, 3022
PspOMI	GGGCCC	1 cut(s)	3020
PstI	CTGCAG	2 cut(s)	1858, 3454
PvuII	CAGCTG	1 cut(s)	256
RsaI	GTAC	5 cut(s)	1423, 1886, 2281, 2842, 3012
RsaNI	GTAC	5 cut(s)	1422, 1885, 2280, 2841, 3011
RseI	CAYNNNNRTG	4 cut(s)	416, 2607, 2661, 3488
SapI	GCTCTTC	1 cut(s)	3504
SaqAI	TTAA	9 cut(s)	1497, 2325, 2337, 2345, 2928, 3143, 3152, 3204, 3486
SchI	GAGTC	9 cut(s)	144, 340, 1412, 1442, 1551, 2285, 2591, 2660, 3475
ScrFI	CCNGG	5 cut(s)	407, 1336, 1359, 1685, 2897
SduI	GDGCHC	2 cut(s)	2637, 3024
SfaNI	GCATC	7 cut(s)	1199, 2290, 2380, 2554, 2575, 3018, 3346
SfcI	CTRYAG	3 cut(s)	1854, 2476, 3450
SfoI	GGCGCC	2 cut(s)	298, 1011
SinI	GGWCC	5 cut(s)	267, 1176, 1955, 2524, 2653
SmiMI	CAYNNNNRTG	4 cut(s)	416, 2607, 2661, 3488
SmlI	CTYRAG	5 cut(s)	348, 911, 1652, 2816, 3136
SmoI	CTYRAG	5 cut(s)	348, 911, 1652, 2816, 3136
SseBI	AGGCCT	1 cut(s)	3195
SspDI	GGCGCC	2 cut(s)	296, 1009
SspI	AATATT	2 cut(s)	967, 1374
SspMI	CTAG	5 cut(s)	779, 1164, 1293, 1689, 3239
StuI	AGGCCT	1 cut(s)	3195
StyD4I	CCNGG	5 cut(s)	405, 1334, 1357, 1683, 2895
StyI	CCWWGG	7 cut(s)	522, 1163, 2463, 2859, 2998, 3084, 3196
TaaI	ACNGT	8 cut(s)	1006, 2068, 2096, 2119, 2777, 2845, 3130, 3430
TaiI	ACGT	3 cut(s)	387, 1850, 2268
TaqI	TCGA	7 cut(s)	170, 333, 489, 723, 1453, 1558, 1963
TaqII	GACCGA	2 cut(s)	284, 437
TatI	WGTACW	2 cut(s)	1884, 3010
TauI	GCSGC	7 cut(s)	134, 235, 253, 688, 851, 1048, 2457
TfiI	GAWTC	9 cut(s)	19, 283, 995, 1123, 1159, 1330, 1560, 1910, 3412
Tru1I	TTAA	9 cut(s)	1497, 2325, 2337, 2345, 2928, 3143, 3152, 3204, 3486
Tru9I	TTAA	9 cut(s)	1497, 2325, 2337, 2345, 2928, 3143, 3152, 3204, 3486
TscAI	CASTG	6 cut(s)	37, 1753, 2118, 3043, 3133, 3454
TseFI	GTSAC	4 cut(s)	1748, 2494, 2663, 3302
Tsp45I	GTSAC	4 cut(s)	1748, 2494, 2663, 3302
TspRI	CASTG	6 cut(s)	37, 1753, 2118, 3043, 3133, 3454
Van91I	CCANNNNNTGG	2 cut(s)	3122, 3334
VpaK11BI	GGWCC	5 cut(s)	267, 1176, 1955, 2524, 2653
XagI	CCTNNNNNAGG	1 cut(s)	491
XapI	RAATTY	6 cut(s)	1260, 1352, 1608, 1982, 2050, 3252
XbaI	TCTAGA	1 cut(s)	3238
XceI	RCATGY	1 cut(s)	2960
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1315
XmaJI	CCTAGG	1 cut(s)	1163
XmnI	GAANNNNTTC	3 cut(s)	1914, 2946, 2976
XspI	CTAG	5 cut(s)	779, 1164, 1293, 1689, 3239
ZraI	GACGTC	1 cut(s)	385

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.