AT1G50710

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002315
Physical Location & Seq
Reverse (-)
18784509 .. 18787802
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50710.1

Sequence Viewer

Length: 1272 bp
ATGGTGAAGGCACTGCAAGGCGCGGCTCAGAATCTACCGGCGGATGTCAACCAATTGATCGATCAGTTAGAGCGTCACTGTTTGGCTCCCGATGGATCTCTTGTTACCAAATCTGTCTACTCCGATCTCCAACTCGCGAGAGAGGAGATGTCTCGGGAGAGACTTCGGTATTTGGAAGCTATGGCTATTTATTGTGAAGCTGTAGCTATGGTTGAAGAATATCAGCAAGCTATTTCAGTAGCTAACCATGGGGGAATTCGTGATGTTCAAGGTCTATACCCTCAACTTGGCTTGAAGAATTCTCCTCAGGTTTATGAGACTCTAGAGCATAGGTTGGTGGTTGCTGAGGCGGCTCAGAAACTGAGGCTACCTCTTATTTCAGATGGTGGTGAAATTCATGAGGAAGAGATTGAAAAGTGGAGTATACTTTCAAGAAGCTCTCTTGATAGTGCAAGCACGAGTTTTACGATTAGCTCCACTTCTAACTCCGTGAACTATGCGAATAGCTCTGCAAATAGTGTTGCTGGTGGGATTAGTCTTAGTGCTGTTGATACTGACGTAGTAGGTGGTGTTCCGAATCGATTTCTTGGAATAACACCTGCTTACTTATCCTATGTTCAGCTTCAAAATACTATTTCCATGGACATGGCTGACTACCAAATGTTTCTTGCCCGTGAGATAGAAGGTCGATTGAAGGAAAAATGTGATAAGTTGGCTGATGCCATTGTTGATGACACTGATTCGTCCACAGGAAATCGAAATTCAAGTGCTAGGCTCCCAGAAAGGGTTAAGTTTATAATTGAGGAGATTGAAAGAGATGAAGCAGCTCTACGAGAGGACCTGTACTCGGCTGACAGGAAGTTTGCTGAATATTACAATGTCCTGGAACAAATACTCGGGGTACTTATAAAGCTTGTGAAGGATCTAAAGTTAGAACATCAACACAAATATAATGAGATGCAGAAGACTTGGTTGTGTAAAAGGTGTGAGACCATGAATGCAAAATTAAGGGTTTTGGAAAATGTTCTCCTCCTTGAGACATACACCCCTGACTCCATATCAGCTCTGCACAACATCAGGAATTATCTAGTCGAGGCTACCGAGGAAGCTTCAGCTGCATACAACAAAGCGGTTACTCGGCTTAGAGAATATCAAGGAGTAGATCCGCATTTTGATACAATTGCCAGACAGTACCATGACATTGTTAAGAAACTGGAAAATATGCAGTGGACCATTCATCAAGTAGAAATGGACCTTAAGTCTCATGACTGA

Protein Analysis

423

Amino Acids

47.77

Weight (kDa)

5.1

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 188 - 417 4.1e-81 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 797, 908
AarI CACCTGC 1 cut(s) 607
Acc36I ACCTGC 1 cut(s) 607
AccI GTMKAC 2 cut(s) 117, 424
AccII CGCG 2 cut(s) 23, 137
AciI CCGC 5 cut(s) 23, 41, 350, 1130, 1166
AclWI GGATC 3 cut(s) 103, 930, 1157
AcsI RAATTY 4 cut(s) 255, 298, 393, 760
AcuI CTGAAG 1 cut(s) 1095
AfaI GTAC 3 cut(s) 845, 903, 1193
AfiI CCNNNNNNNGG 3 cut(s) 287, 784, 847
AflII CTTAAG 1 cut(s) 1256
AgsI TTSAA 9 cut(s) 215, 269, 295, 413, 432, 626, 694, 765, 812
AhdI GACNNNNNGTC 1 cut(s) 1258
AjnI CCWGG 1 cut(s) 882
Alw26I GTCTC 6 cut(s) 154, 156, 311, 983, 1031, 1266
AlwI GGATC 3 cut(s) 103, 930, 1157
AlwNI CAGNNNCTG 1 cut(s) 361
Ama87I CYCGRG 2 cut(s) 153, 896
ApeKI GCWGC 2 cut(s) 824, 1115
ApoI RAATTY 4 cut(s) 255, 298, 393, 760
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 1 cut(s) 1023
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 3 cut(s) 838, 1230, 1252
AsuHPI GGTGA 2 cut(s) 16, 401
AvaI CYCGRG 2 cut(s) 153, 896
AvaII GGWCC 3 cut(s) 838, 1230, 1252
AxyI CCTNAGG 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 543, 576
BarI GAAGNNNNNNTAC 2 cut(s) 813, 845
BauI CACGAG 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 971
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 836, 1102
BccI CCATC 2 cut(s) 86, 377
BciT130I CCWGG 1 cut(s) 884
BcoDI GTCTC 6 cut(s) 154, 156, 311, 983, 1031, 1266
BfaI CTAG 3 cut(s) 323, 771, 1088
BfmI CTRYAG 1 cut(s) 201
BfrI CTTAAG 1 cut(s) 1256
BfuAI ACCTGC 1 cut(s) 607
BisI GCNGC 4 cut(s) 24, 351, 825, 1116
BlsI GCNGC 4 cut(s) 25, 352, 826, 1117
Bme1390I CCNGG 1 cut(s) 884
Bme18I GGWCC 3 cut(s) 838, 1230, 1252
BmeRI GACNNNNNGTC 1 cut(s) 1258
BmeT110I CYCGRG 2 cut(s) 153, 896
BmgT120I GGNCC 3 cut(s) 838, 1230, 1252
BmiI GGNNCC 2 cut(s) 87, 776
BmrFI CCNGG 1 cut(s) 884
BmsI GCATC 2 cut(s) 709, 948
BpiI GAAGAC 1 cut(s) 971
BplI GAGNNNNNCTC 2 cut(s) 355, 387
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 1055
Bsa29I ATCGAT 2 cut(s) 60, 580
BsaI GGTCTC 1 cut(s) 983
BsaJI CCNNGG 3 cut(s) 247, 639, 1101
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 784, 847
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 1218
Bse21I CCTNAGG 1 cut(s) 306
BseBI CCWGG 1 cut(s) 884
BseCI ATCGAT 2 cut(s) 60, 580
BseDI CCNNGG 3 cut(s) 247, 639, 1101
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 3 cut(s) 287, 784, 847
BseMII CTCAG 5 cut(s) 41, 320, 336, 353, 368
BseNI ACTGG 1 cut(s) 1218
BseRI GAGGAG 4 cut(s) 158, 294, 818, 1019
BseXI GCAGC 2 cut(s) 836, 1102
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 2 cut(s) 23, 137
BshVI ATCGAT 2 cut(s) 60, 580
BsiHKCI CYCGRG 2 cut(s) 153, 896
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 287, 784, 847
BsmAI GTCTC 6 cut(s) 154, 156, 311, 983, 1031, 1266
BsmI GAATGC 1 cut(s) 1003
Bso31I GGTCTC 1 cut(s) 983
BsoBI CYCGRG 2 cut(s) 153, 896
Bsp143I GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
Bsp19I CCATGG 2 cut(s) 247, 639
Bsp68I TCGCGA 1 cut(s) 137
BspACI CCGC 5 cut(s) 23, 41, 350, 1130, 1166
BspCNI CTCAG 5 cut(s) 40, 319, 337, 354, 367
BspDI ATCGAT 2 cut(s) 60, 580
BspFNI CGCG 2 cut(s) 23, 137
BspHI TCATGA 2 cut(s) 397, 1264
BspLI GGNNCC 2 cut(s) 87, 776
BspMI ACCTGC 1 cut(s) 607
BspPI GGATC 3 cut(s) 103, 930, 1157
BspTI CTTAAG 1 cut(s) 1256
BspTNI GGTCTC 1 cut(s) 983
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 1218
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 247, 639, 1101
BssMI GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
BssNAI GTATAC 1 cut(s) 425
BssSI CACGAG 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 247, 639
Bst1107I GTATAC 1 cut(s) 425
Bst2BI CACGAG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 2 cut(s) 80, 1191
Bst6I CTCTTC 1 cut(s) 399
BstAFI CTTAAG 1 cut(s) 1256
BstC8I GCNNGC 2 cut(s) 228, 454
BstDEI CTNAG 7 cut(s) 27, 306, 345, 354, 362, 539, 1142
BstDSI CCRYGG 2 cut(s) 247, 639
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 2 cut(s) 23, 137
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 6 cut(s) 60, 64, 98, 127, 925, 1165
BstMAI GTCTC 6 cut(s) 154, 156, 311, 983, 1031, 1266
BstMBI GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
BstMWI GCNNNNNNNGC 2 cut(s) 350, 1115
BstNI CCWGG 1 cut(s) 884
BstSCI CCNGG 1 cut(s) 882
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 2 cut(s) 23, 137
BstV1I GCAGC 2 cut(s) 836, 1102
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 3 cut(s) 95, 922, 1162
BstXI CCANNNNNNTGG 1 cut(s) 646
BstYI RGATCY 3 cut(s) 95, 922, 1162
BstZ17I GTATAC 1 cut(s) 425
Bsu15I ATCGAT 2 cut(s) 60, 580
Bsu36I CCTNAGG 1 cut(s) 306
BsuTUI ATCGAT 2 cut(s) 60, 580
BtgI CCRYGG 2 cut(s) 247, 639
BtsCI GGATG 1 cut(s) 49
BtsI GCAGTG 2 cut(s) 11, 1232
BtsIMutI CAGTG 4 cut(s) 11, 76, 735, 1232
BtuMI TCGCGA 1 cut(s) 137
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 2 cut(s) 228, 454
CaiI CAGNNNCTG 1 cut(s) 361
CciI TCATGA 2 cut(s) 397, 1264
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 838, 1230, 1252
ClaI ATCGAT 2 cut(s) 60, 580
CseI GACGC 1 cut(s) 62
Csp6I GTAC 3 cut(s) 844, 902, 1192
CviAII CATG 7 cut(s) 248, 398, 640, 646, 994, 1196, 1265
CviQI GTAC 3 cut(s) 844, 902, 1192
DdeI CTNAG 7 cut(s) 27, 306, 345, 354, 362, 539, 1142
DpnI GATC 6 cut(s) 59, 63, 97, 126, 924, 1164
DpnII GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
DriI GACNNNNNGTC 1 cut(s) 1258
Eam1104I CTCTTC 1 cut(s) 399
Eam1105I GACNNNNNGTC 1 cut(s) 1258
EarI CTCTTC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 247, 639
Eco31I GGTCTC 1 cut(s) 983
Eco47I GGWCC 3 cut(s) 838, 1230, 1252
Eco57I CTGAAG 1 cut(s) 1095
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 153, 896
EcoO109I RGGNCCY 1 cut(s) 838
EcoRI GAATTC 2 cut(s) 255, 298
EcoRII CCWGG 1 cut(s) 882
EcoT14I CCWWGG 2 cut(s) 247, 639
ErhI CCWWGG 2 cut(s) 247, 639
FaeI CATG 7 cut(s) 251, 401, 643, 649, 997, 1199, 1268
FatI CATG 7 cut(s) 247, 397, 639, 645, 993, 1195, 1264
FblI GTMKAC 2 cut(s) 117, 424
Fnu4HI GCNGC 4 cut(s) 24, 351, 825, 1116
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 4 cut(s) 24, 351, 825, 1116
FspBI CTAG 3 cut(s) 323, 771, 1088
GlaI GCGC 1 cut(s) 22
GluI GCNGC 4 cut(s) 24, 351, 825, 1116
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 23
Hin1II CATG 7 cut(s) 251, 401, 643, 649, 997, 1199, 1268
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 911, 1107
HinfI GANTC 5 cut(s) 31, 319, 577, 740, 1052
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 16, 401
Hpy166II GTNNAC 6 cut(s) 49, 118, 425, 493, 747, 1230
Hpy188I TCNGA 5 cut(s) 30, 124, 357, 382, 576
Hpy8I GTNNAC 6 cut(s) 49, 118, 425, 493, 747, 1230
HpyAV CCTTC 3 cut(s) 677, 688, 913
HpyCH4III ACNGT 2 cut(s) 80, 1191
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 8 cut(s) 16, 452, 512, 961, 1001, 1069, 1118, 1225
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 1115
HpyF3I CTNAG 7 cut(s) 27, 306, 345, 354, 362, 539, 1142
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 7 cut(s) 251, 401, 643, 649, 997, 1199, 1268
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
LmnI GCTCC 3 cut(s) 91, 479, 780
Lsp1109I GCAGC 2 cut(s) 836, 1102
LweI GCATC 2 cut(s) 709, 948
MaeI CTAG 3 cut(s) 323, 771, 1088
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 3 cut(s) 74, 103, 1132
MalI GATC 6 cut(s) 59, 63, 97, 126, 924, 1164
MboI GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
MboII GAAGA 4 cut(s) 227, 307, 416, 976
MfeI CAATTG 2 cut(s) 53, 1179
MflI RGATCY 3 cut(s) 95, 922, 1162
MluCI AATT 9 cut(s) 53, 255, 298, 393, 760, 798, 1004, 1081, 1179
MlyI GAGTC 2 cut(s) 313, 1046
MmeI TCCRAC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 1023
MseI TTAA 4 cut(s) 789, 1007, 1206, 1257
MslI CAYNNNNRTG 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 1115
MspCI CTTAAG 1 cut(s) 1256
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 884
MunI CAATTG 2 cut(s) 53, 1179
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 1 cut(s) 884
MvnI CGCG 2 cut(s) 23, 137
MwoI GCNNNNNNNGC 2 cut(s) 350, 1115
NcoI CCATGG 2 cut(s) 247, 639
NdeII GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
NlaIII CATG 7 cut(s) 251, 401, 643, 649, 997, 1199, 1268
NlaIV GGNNCC 2 cut(s) 87, 776
NmeAIII GCCGAG 2 cut(s) 827, 1117
NmuCI GTSAC 1 cut(s) 74
NruI TCGCGA 1 cut(s) 137
PagI TCATGA 2 cut(s) 397, 1264
PaqCI CACCTGC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 2 cut(s) 464, 1098
PctI GAATGC 1 cut(s) 1003
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 3 cut(s) 31, 577, 740
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 4 cut(s) 25, 352, 826, 1117
PleI GAGTC 2 cut(s) 313, 1046
PpsI GAGTC 2 cut(s) 313, 1046
PpuMI RGGWCCY 1 cut(s) 838
PsiI TTATAA 2 cut(s) 797, 908
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 1 cut(s) 882
PspGI CCWGG 1 cut(s) 882
PspN4I GGNNCC 2 cut(s) 87, 776
PspPI GGNCC 3 cut(s) 838, 1230, 1252
PspPPI RGGWCCY 1 cut(s) 838
PsrI GAACNNNNNNTAC 2 cut(s) 555, 587
PstNI CAGNNNCTG 1 cut(s) 361
PsuI RGATCY 3 cut(s) 95, 922, 1162
PvuII CAGCTG 1 cut(s) 1115
RruI TCGCGA 1 cut(s) 137
RsaI GTAC 3 cut(s) 845, 903, 1193
RsaNI GTAC 3 cut(s) 844, 902, 1192
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 4 cut(s) 789, 1007, 1206, 1257
SatI GCNGC 4 cut(s) 24, 351, 825, 1116
Sau3AI GATC 6 cut(s) 57, 61, 95, 124, 922, 1162
Sau96I GGNCC 3 cut(s) 838, 1230, 1252
SchI GAGTC 2 cut(s) 313, 1046
ScrFI CCNGG 1 cut(s) 884
SfaNI GCATC 2 cut(s) 709, 948
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 3 cut(s) 838, 1230, 1252
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 1034, 1256
SmoI CTYRAG 2 cut(s) 1034, 1256
Sse9I AATT 9 cut(s) 53, 255, 298, 393, 760, 798, 1004, 1081, 1179
SsiI CCGC 5 cut(s) 23, 41, 350, 1130, 1166
SspI AATATT 1 cut(s) 872
SspMI CTAG 3 cut(s) 323, 771, 1088
StyD4I CCNGG 1 cut(s) 882
StyI CCWWGG 2 cut(s) 247, 639
TaaI ACNGT 2 cut(s) 80, 1191
TaiI ACGT 1 cut(s) 561
TaqI TCGA 5 cut(s) 60, 580, 688, 757, 1092
TasI AATT 9 cut(s) 53, 255, 298, 393, 760, 798, 1004, 1081, 1179
TatI WGTACW 1 cut(s) 843
TauI GCSGC 2 cut(s) 26, 353
TfiI GAWTC 3 cut(s) 31, 577, 740
Tru1I TTAA 4 cut(s) 789, 1007, 1206, 1257
Tru9I TTAA 4 cut(s) 789, 1007, 1206, 1257
TscAI CASTG 4 cut(s) 18, 83, 742, 1232
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 2 cut(s) 824, 1115
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 4 cut(s) 386, 834, 1010, 1226
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 4 cut(s) 18, 83, 742, 1232
Vha464I CTTAAG 1 cut(s) 1256
VpaK11BI GGWCC 3 cut(s) 838, 1230, 1252
XapI RAATTY 4 cut(s) 255, 298, 393, 760
XbaI TCTAGA 1 cut(s) 322
XmiI GTMKAC 2 cut(s) 117, 424
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 3 cut(s) 323, 771, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.